PI0009445 (gene) Melon (PI 482460) v1

Overview


Name	PI0009445
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Cytochrome P450
Location	chr04: 19007327 .. 19010466 (+)
RNA-Seq Expression	PI0009445
Synteny	PI0009445

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAATTTACCTAACAATCTTTTTCTGTCTTCTAATCCCCATCTTCCTTTTCCTTAGAAGATCATCCAAAAAGCAGCAGCTTCCTCCAGGTTCGCTAGGGTTTCCATTGATCGGTCAGAGCCTAAGTCTCCTCCGCGCCATGCGAACCAACACCGCTGAACAATGGGTGCAGCAGAGAATCAGAAACTACGGGGCCATCTCCAAGCTAACCCTATTCGGAAAGCCTACTGTGTTCATCGGTGGGCAGAGCGGGAACAAGTTGATTTTCAGCGGGGATTGCGCAGTAGTTTCGAATCAGCAAAATGAGTCTCTTAGGGCAATTTTAGGGGAAAGGAATTTGTTGGAGCTGATTGGGGAAGATCATAAGCGTGTGAGAAATGCGTTGGTTTCGTTCTTGAAGCCGGAATGTTTGAAGGAATATGTGGGCAAAATGGATGAAGAAATCAGAGCACATATCAACATGCATTGGCACAGTAAAGAAGAGGTTACTGTGAGTACACCTTTCCTTTTTTCCTTTTTCTTTTTTCTTTTGTTTTTCAAAATTGAAAATTGGCATTTCAATTTTCATTTCTGAAAATAGGAAAATTTTGTTTTTTGGGTACTCAACAGATGTAACATCTCTACTGTTGTTAAGAAAAGTAAATAAATAAAGGACCCCGTTGCCGGGGATAAAAAACGGGTCACCCGCATGACAGGCGGGAATACTCACTGCTATTATACTATTCCCTGCCATAATACAAAGACTTAATTTGTCAAGTTAACTAAATAAAGAATCTATAAAACAAATAAGTATGTTGAAGAAAATTTATGGAAAATTTACGTAAATGTAACAAAATCCCAAAATATTTACAACTCGTATAACAAAATAAAAGGTCCATAAAATCGACCATTTTTTTAAAATATTTCAGGCTTGCCCTTGCCGCTCTTGAATTTCTTGCGGCTCCGATTTTTTCCTTCTTATTCGTTATATTTCTTCTTTCTTTCTTTTCATTTTCTTCACAGTGGCGGTCACTGGCGGTGTTCTCCTTCTTCTGCAATTTTTCTATTATATTTTTTTGGAATCATGATAATTGTTCTATAATAATTATTATTATTTTCATTATACTTCTGCTATTATTATCTTTGTTTTCAATTAATCATCAATCTTGTATTATTTTTTTTCAATGTGTCGTGGATAGAGATGAACTACATGACCGTTTACCATAGGGCTTTTCAAAACCATCGCTTATCATGAACTATACAATCGTGTATCATGAACTACACATGATCGTTTATCATGATCTTCACGATCGTTTACTAGGGCTTTTCAAAACCATCATTTATCATGAACTAACAATCATATATCATGAACTACGCGATCATGTATGATATATTACACAATCGTGTATCATGAACTACACGATCGTGTATGATAAACTACACGATCGTGTATCATCATCTACACGGTCGTTTACTAGAGCTTTTCAAAACCATCGTTTATCATAAACTACACGATCGTGTATCATGATCTACACGATCATTTGAAAGAAGTGCACGTGTGTGGCCAATTAATCACGTGTTGACTAGGGTACTTTTAGTATTTCACATTGTGGGCGTATGGGCTTTTCCGTTTTTCAAAATTGTTTTATACAGTATAAATATTTTACCGGTTTGTTATATTTTTAAAAATACCCCAAAAATTATTAGTAGTGAAAATAAAAGAGAAAGAAAAAAAGTTATAAAATAATTAATAAAATGTTTTTGAGTCTTAGTTACATTTGAAAGGTTTAAAAATTATTCATAGAAATTACAAAATTTAAACAAAGACTCAAAAGTTTAGATCTTTATGAGATACTTTTCCCTTTTTCTTTTATTATGTTGAGGAACCTATATAAATGTACAAAATTGAGAGTTGAATGTGTTATTATAATTATGATGCTTTTTTCTATTGAGATGATGTTTGCTATGCACATAGGTATTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGTTCTCTTCTGTTTGGTCTTGAGCAAGGTATGAGAAGAGAAGGAATGATAGAATGCTTTCGAGTAATGATAACCGGAGTATGGTCGATCCCGATAAACTTGCCGTTCACAAGGTACAACCAAAGCCAACGTGCGAGTAGGAAAATTCAAGAGATGCTCAAGGAACTGTTGGATGAAAAGAGAGTTGAATTAGAAGAAAAAGGAGGTTCCTCACACCAAGATCTAATTACTTGTTTGTTAAGCATAAGGAATGAAGAAAATGAACTAGTATTGAGTGAAAAGGAAATTGTGCACAATATTATGTTGGTAATGGTTGCTGGATTTGATACTTCATCAGTTCTCATTACGTTCATGATGCGGAATTTAGCTAATAATCCAACTGTTTATGCAACTGTTCTTCAAGGTATGCTGCTTCCAATTATCTCATGCACTCTATAGTACAATTATGACTTTATATATATATATATAACCATATATTTATATTTCTTCAAGCTTATTTGGTTTTTGTTGTCGTGTGTGTGGTTACAGAACAAGAAGAGATAGCAAGAAATAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTACCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAGGTAAGTCAAATTTTCTTCCCTTTTTTTCTATTTAAAGTTTTTTGACTTGAGGATCTCAAGTGAAACTTCATGAAACATCTTTAAACGTACTCATCCAACAACATTATTGGATTTCAGATATTTTGGGCATCACCAGTTACTCATATGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCTAATAGGTTTGAAAATCCAGCGTCGATTCCACCGTATTGCTTCCTTGGATTTGGCAGTGGTCTGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACTCAATTCACATGGAAACTACTTTTGGATGACCATTTTATTAGAGATCCAATGCCAACACCTACCAAAGGGATGCCTATCAAAATTTGCCCCAGACAAACTTTCATGGCATTGTAG

mRNA sequence

ATGACAATTTACCTAACAATCTTTTTCTGTCTTCTAATCCCCATCTTCCTTTTCCTTAGAAGATCATCCAAAAAGCAGCAGCTTCCTCCAGGTTCGCTAGGGTTTCCATTGATCGGTCAGAGCCTAAGTCTCCTCCGCGCCATGCGAACCAACACCGCTGAACAATGGGTGCAGCAGAGAATCAGAAACTACGGGGCCATCTCCAAGCTAACCCTATTCGGAAAGCCTACTGTGTTCATCGGTGGGCAGAGCGGGAACAAGTTGATTTTCAGCGGGGATTGCGCAGTAGTTTCGAATCAGCAAAATGAGTCTCTTAGGGCAATTTTAGGGGAAAGGAATTTGTTGGAGCTGATTGGGGAAGATCATAAGCGTGTGAGAAATGCGTTGGTTTCGTTCTTGAAGCCGGAATGTTTGAAGGAATATGTGGGCAAAATGGATGAAGAAATCAGAGCACATATCAACATGCATTGGCACAGTAAAGAAGAGGTTACTGTATTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGTTCTCTTCTGTTTGGTCTTGAGCAAGGTATGAGAAGAGAAGGAATGATAGAATGCTTTCGAGTAATGATAACCGGAGTATGGTCGATCCCGATAAACTTGCCGTTCACAAGGTACAACCAAAGCCAACGTGCGAGTAGGAAAATTCAAGAGATGCTCAAGGAACTGTTGGATGAAAAGAGAGTTGAATTAGAAGAAAAAGGAGGTTCCTCACACCAAGATCTAATTACTTGTTTGTTAAGCATAAGGAATGAAGAAAATGAACTAGTATTGAGTGAAAAGGAAATTGTGCACAATATTATGTTGGTAATGGTTGCTGGATTTGATACTTCATCAGTTCTCATTACGTTCATGATGCGGAATTTAGCTAATAATCCAACTGTTTATGCAACTGTTCTTCAAGAACAAGAAGAGATAGCAAGAAATAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTACCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAGATATTTTGGGCATCACCAGTTACTCATATGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCTAATAGGTTTGAAAATCCAGCGTCGATTCCACCGTATTGCTTCCTTGGATTTGGCAGTGGTCTGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACTCAATTCACATGGAAACTACTTTTGGATGACCATTTTATTAGAGATCCAATGCCAACACCTACCAAAGGGATGCCTATCAAAATTTGCCCCAGACAAACTTTCATGGCATTGTAG

Coding sequence (CDS)

ATGACAATTTACCTAACAATCTTTTTCTGTCTTCTAATCCCCATCTTCCTTTTCCTTAGAAGATCATCCAAAAAGCAGCAGCTTCCTCCAGGTTCGCTAGGGTTTCCATTGATCGGTCAGAGCCTAAGTCTCCTCCGCGCCATGCGAACCAACACCGCTGAACAATGGGTGCAGCAGAGAATCAGAAACTACGGGGCCATCTCCAAGCTAACCCTATTCGGAAAGCCTACTGTGTTCATCGGTGGGCAGAGCGGGAACAAGTTGATTTTCAGCGGGGATTGCGCAGTAGTTTCGAATCAGCAAAATGAGTCTCTTAGGGCAATTTTAGGGGAAAGGAATTTGTTGGAGCTGATTGGGGAAGATCATAAGCGTGTGAGAAATGCGTTGGTTTCGTTCTTGAAGCCGGAATGTTTGAAGGAATATGTGGGCAAAATGGATGAAGAAATCAGAGCACATATCAACATGCATTGGCACAGTAAAGAAGAGGTTACTGTATTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGTTCTCTTCTGTTTGGTCTTGAGCAAGGTATGAGAAGAGAAGGAATGATAGAATGCTTTCGAGTAATGATAACCGGAGTATGGTCGATCCCGATAAACTTGCCGTTCACAAGGTACAACCAAAGCCAACGTGCGAGTAGGAAAATTCAAGAGATGCTCAAGGAACTGTTGGATGAAAAGAGAGTTGAATTAGAAGAAAAAGGAGGTTCCTCACACCAAGATCTAATTACTTGTTTGTTAAGCATAAGGAATGAAGAAAATGAACTAGTATTGAGTGAAAAGGAAATTGTGCACAATATTATGTTGGTAATGGTTGCTGGATTTGATACTTCATCAGTTCTCATTACGTTCATGATGCGGAATTTAGCTAATAATCCAACTGTTTATGCAACTGTTCTTCAAGAACAAGAAGAGATAGCAAGAAATAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTACCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAGATATTTTGGGCATCACCAGTTACTCATATGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCTAATAGGTTTGAAAATCCAGCGTCGATTCCACCGTATTGCTTCCTTGGATTTGGCAGTGGTCTGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACTCAATTCACATGGAAACTACTTTTGGATGACCATTTTATTAGAGATCCAATGCCAACACCTACCAAAGGGATGCCTATCAAAATTTGCCCCAGACAAACTTTCATGGCATTGTAG

Protein sequence

MTIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSLLFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLANNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICPRQTFMAL

Homology

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BLAST of PI0009445 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.8e-100
Identity = 190/471 (40.34%), Postives = 282/471 (59.87%), Query Frame = 0


Query: 2   TIYLTIFFCLLIPIFLFLRRSSKK-QQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 61
           T++L  F  L    +L     +K  + +PPG+ G+PLIG++L  L   R      +   R
Sbjct: 24  TVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSR 83

Query: 62  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 121
            + YG +   +L G PTV      GN+ +FS +  +V N    S+   L   +L+  +G+
Sbjct: 84  TQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSV-GNLFRSSLITTVGD 143

Query: 122 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 181
           D KR+R  L++FL+PE L+E+VG++D   + H+  HW  K+EV  LPL+K  TF++ C L
Sbjct: 144 DAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDL 203

Query: 182 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 241
              +      + +   F V + GV  IPI+LP TRYN+++ A+  I++ L  +++E+++ 
Sbjct: 204 FASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIA 263

Query: 242 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 301
           LE    S  QDL++ LLS  +E+ E  L++ EI  NI+L++ AG DTSS  +T +++ LA
Sbjct: 264 LEAGNASPEQDLLSFLLSNVDEQGE-SLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLA 323

Query: 302 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 361
            NP  Y  VL+EQ  IA +K+ G+LL WEDL +MKY+WRVA E LRL   + G FRKA+ 
Sbjct: 324 ENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIK 383

Query: 362 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 421
           +  + G+ IPKGW++ W    TH     F  P KFDP+RFE     PPY F+ FG G R+
Sbjct: 384 EFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGP-PPYTFVPFGGGPRM 443

Query: 422 CPGNEFARVETLVTIHYLITQFTWKLLLD-DHFIRDPMPTPTKGMPIKICP 471
           CPGNEFAR+E L+ +H ++  F W L+   +  I DPMP P  G+PIK+ P
Sbjct: 444 CPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

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BLAST of PI0009445 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 8.9e-100
Identity = 190/474 (40.08%), Postives = 282/474 (59.49%), Query Frame = 0


Query: 2   TIYLTIFFCLLIPIFLFLRRSSKK-QQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 61
           T++L  F  L    +L     +K  + +PPG+ G+PLIG++L  L   R      +   R
Sbjct: 24  TVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSR 83

Query: 62  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 121
            + YG +   +L G PTV      GN+ +FS +  +V N    S+   L   +L+  +G+
Sbjct: 84  TQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSV-GNLFRSSLITTVGD 143

Query: 122 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 181
           D KR+R  L++FL+PE L+E+VG++D   + H+  HW  K+EVT LPL+K  TF++ C L
Sbjct: 144 DAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDL 203

Query: 182 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 241
              +        +   F V + GV  IPI+LP TRYN+++ A+  I++ L  +++E+++ 
Sbjct: 204 FASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIG 263

Query: 242 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 301
           LE    S  QDL++ LLS  +E+ E  L++ EI  NI+L++ AG DTSS  +T +++ LA
Sbjct: 264 LEAGNASPEQDLLSFLLSNVDEQGE-SLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLA 323

Query: 302 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 361
            NP  Y  VL+EQ +IA +K+ G+LL WEDL +MKY+WRVA E LRL     G FRKA+ 
Sbjct: 324 ENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIK 383

Query: 362 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 421
           +  + G+ IPKGW+++W    TH     F  P  FDP+RFE     PPY F+ FG G R+
Sbjct: 384 EFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGP-PPYTFVPFGGGPRM 443

Query: 422 CPGNEFARVETLVTIHYLITQFTWKLL-LDDHFIRDPMPTPTKGMPIKICPRQT 474
           CPGNEFAR+E LV +H ++    W L+   +  I DPMP P  G+PIK+ P  +
Sbjct: 444 CPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDS 494

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BLAST of PI0009445 vs. ExPASy Swiss-Prot
Match: A0A0U2U8U5 (Beta-amyrin 28-monooxygenase OS=Barbarea vulgaris OX=50459 GN=CYP716A81 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.0e-95
Identity = 182/481 (37.84%), Postives = 292/481 (60.71%), Query Frame = 0


Query: 3   IYLTIFFCLLIPIFLFLRRSSKK-------QQLPPGSLGFPLIGQSLSLLRAMRTNTAEQ 62
           +YLTI F  +  I L L    +K       Q LPPG  GFPLIG+SLS L   R    E+
Sbjct: 1   MYLTILFLFVSSILLSLMFLLRKHLSHFSYQNLPPGKTGFPLIGESLSFLSEGRQGRPEK 60

Query: 63  WVQQRIRNY----GAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGE 122
           +V  R+R +      + K  LFG PT  + G SGNK +F+ +  +V +   +S++ I   
Sbjct: 61  FVTDRVRRFISSSTGVFKTHLFGYPTAVMTGASGNKFLFTNENKLVVSWWPDSVKKIFPY 120

Query: 123 RNLLELIGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKT 182
                   ++ K++R  L+ F+KPE L++Y+G MDE  + H    W +++++ V PL K 
Sbjct: 121 TQ--STYTDESKKLRILLMQFMKPEALRKYIGVMDEVAQRHFETLWTNQKQLIVYPLSKK 180

Query: 183 LTFNIVCSLLFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLK 242
           LTF++ C L   ++   R   + + F  ++TG++S+PI+LP TR+N+S +ASR +++ + 
Sbjct: 181 LTFSVACRLFLSMDDPERVSKLEDRFNSVVTGIYSVPIDLPGTRFNRSIKASRLLRKEVC 240

Query: 243 ELLDEKRVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVL 302
            ++ ++R EL+    S+ QD+++ +L    E       ++++ + ++ +++ G DT+++ 
Sbjct: 241 AIIGQRREELKAGRASAEQDVLSHMLMSVGE-----TKDEDLANYLIGILIGGHDTAAIA 300

Query: 303 ITFMMRNLANNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPI 362
            TF++  LA  P VY  VLQEQ+EI + KK  E L WED+ KMKY+W VA E +RLV P+
Sbjct: 301 TTFIINYLAEYPHVYQRVLQEQKEILKEKKEEERLKWEDIEKMKYSWNVACEVMRLVPPL 360

Query: 363 FGGFRKAMNDIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCF 422
            G FR+A++   F G+ IPKGW+++W++  THM+   F EP +F+PNRFE  +   PY +
Sbjct: 361 TGNFREAIDHFTFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEG-SGPKPYSY 420

Query: 423 LGFGSGLRICPGNEFARVETLVTIHYLITQFTWKLLL--DDHFIRDPMPTPTKGMPIKIC 471
           + FG G R+CPG EFAR+E LV IH L+ +F W+ +   ++  + DP+P P  G+PI+I 
Sbjct: 421 IPFGGGPRMCPGREFARLEILVIIHNLVNRFKWEKVFPNENKIVVDPLPKPGNGLPIRIF 473

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BLAST of PI0009445 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 349.4 bits (895), Expect = 6.6e-95
Identity = 177/474 (37.34%), Postives = 288/474 (60.76%), Query Frame = 0


Query: 2   TIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQRI 61
           +I L+    +L+ + LF  +     +LPPG LG P IG+S   LRA+R+N+ EQ+  +R+
Sbjct: 26  SIALSAIAGILLLLLLFRSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSLEQFFDERV 85

Query: 62  RNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGED 121
           + +G + K +L G PTV + G +GN+LI S +  +V          ++GE ++    GED
Sbjct: 86  KKFGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRGED 145

Query: 122 HKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSLL 181
           H  +R+AL  F  P  L+ Y+GKM+ EI++HIN  W  K+EV VLPL++ L FNI   L 
Sbjct: 146 HIVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILF 205

Query: 182 FGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVEL 241
           F +     ++ + +    ++ G +++PI+LP   ++++ +   K+ +++  L+ +++ +L
Sbjct: 206 FNIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKEDL 265

Query: 242 EEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLAN 301
           +    ++ QDL++ LL+ R+++    L+  EI+ N   ++ A +DT++  +  + + L++
Sbjct: 266 QSGSATATQDLLSVLLTFRDDKG-TPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLSS 325

Query: 302 NPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMND 361
           NP  Y  V+QEQ EI  NK+ GE + W+DL  MKYTW+VA ETLR+  P+FG FRKA+ D
Sbjct: 326 NPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAITD 385

Query: 362 IQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPAS-IPPYCFLGFGSGLRI 421
           IQ+ GY IPKGW++ W +  TH  D  F EP KF P+RF+     + PY FL FG G R 
Sbjct: 386 IQYDGYTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGGQRS 445

Query: 422 CPGNEFARVETLVTIHYLITQFTWKLLL--DDHFIRDPM-PTPTKGMPIKICPR 472
           C G EF+++E L+ +H+ +  F+    +  D+    DP+ P P+KG  IK+ PR
Sbjct: 446 CVGWEFSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

[Close]

BLAST of PI0009445 vs. ExPASy Swiss-Prot
Match: Q9AXM6 (Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 5.6e-94
Identity = 176/469 (37.53%), Postives = 284/469 (60.55%), Query Frame = 0


Query: 7   IFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQRIRNYGA 66
           I   +L+ +F +  RSS K  LPPG LGFPLIG+++ LLR +R+ T +++   R++ +G 
Sbjct: 31  ILAIILLLLFRYNHRSSVK--LPPGKLGFPLIGETIQLLRTLRSETPQKFFDDRLKKFGP 90

Query: 67  ISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVR 126
           +   +L G PTV + G +GNKL+ S +  +V  +  +S   ++GE +++   GEDH+ +R
Sbjct: 91  VYMTSLIGHPTVVLCGPAGNKLVLSNEDKLVEMEGPKSFMKLIGEDSIVAKRGEDHRILR 150

Query: 127 NALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSLLFGLEQ 186
            AL  FL  + L+ Y+G+M  EI  H N  W  K+EV VLPL++ L F+I  +L F +  
Sbjct: 151 TALARFLGAQALQNYLGRMSSEIGHHFNEKWKGKDEVKVLPLVRGLIFSIASTLFFDVND 210

Query: 187 GMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVELEEKGG 246
           G +++ +      ++ G  S+P++ P TRY +  +A  K+ E+L  L+  +R +L     
Sbjct: 211 GHQQKQLHHLLETILVGSLSVPLDFPGTRYRKGLQARLKLDEILSSLIKRRRRDLRSGIA 270

Query: 247 SSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLANNPTVY 306
           S  QDL++ LL+ R+E+    L+++ I+ N   +  A +DT+   +  + + L +NP  +
Sbjct: 271 SDDQDLLSVLLTFRDEKGN-SLTDQGILDNFSAMFHASYDTTVAPMALIFKLLYSNPEYH 330

Query: 307 ATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGG 366
             V QEQ EI  NKK GE ++W+DL  MKYTW+   E+LR+  P+FG FRKA+ DI + G
Sbjct: 331 EKVFQEQLEIIGNKKEGEEISWKDLKSMKYTWQAVQESLRMYPPVFGIFRKAITDIHYDG 390

Query: 367 YLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPA-SIPPYCFLGFGSGLRICPGNE 426
           Y IPKGW++  +   TH+ +  F EP +F P+RFE+    + PY ++ FG GLR CPG E
Sbjct: 391 YTIPKGWRVLCSPYTTHLREEYFPEPEEFRPSRFEDEGRHVTPYTYVPFGGGLRTCPGWE 450

Query: 427 FARVETLVTIHYLITQFTWKLLLD--DHFIRDPM-PTPTKGMPIKICPR 472
           F+++E L+ +H+ +  F+  + +D  +  + DP+ P P  G  IK+ PR
Sbjct: 451 FSKIEILLFVHHFVKNFSSYIPVDPNEKVLSDPLPPLPANGFSIKLFPR 496

[Close]

BLAST of PI0009445 vs. ExPASy TrEMBL
Match: A0A0A0LA02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.0e-263
Identity = 454/478 (94.98%), Postives = 466/478 (97.49%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 60
           M IYLTIFFCLLIPIFLFLRRSSKK+QLPPGSLGFPLIGQSLSLLRAMRTNTAEQW QQR
Sbjct: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIR+HI MHWH K+EVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 240
           LFGLEQG RRE MIECFRVMI+GVWSIPINLPFTRYNQS+RASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLV+VAGFDTSSVLITFMMRNLA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300

Query: 301 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYA VLQEQEEIAR+KKCGELLNWEDL KMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 420
           DIQFGGYLIPKGWQIFW SPVTH+DDTIFREPSKFDP+RF+NPASIPPYCFLGFGSG RI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICPR-QTFMAL 478
           CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKI PR Q+FMA+
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 478

[Close]

BLAST of PI0009445 vs. ExPASy TrEMBL
Match: A0A5A7TZG7 (Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00020 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.5e-262
Identity = 449/476 (94.33%), Postives = 460/476 (96.64%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 60
           M IYLTI FCLLIPIFLFLR SSKKQ LPPGSLGFP IGQSLSLLRAMRTNTAEQW QQR
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL GE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIR+HI+MHWH K+EVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 240
           LFGLEQGMRRE MIECFRVMI+GVWSIPINLPFTRYNQS+RASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMR+LA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLA 300

Query: 301 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYA VLQEQEEIAR+KKCGELLNWEDL KMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 420
           DIQFGGYLIPKGWQIFW SPVTH+DD IFREPSKFDP+RF+NPASIPPYCFLGFGSG RI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICPRQTFMA 477
           CPGNEFARVETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKI PRQTFMA
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTFMA 476

[Close]

BLAST of PI0009445 vs. ExPASy TrEMBL
Match: A0A1S3C4V0 (cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.5e-262
Identity = 449/476 (94.33%), Postives = 460/476 (96.64%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 60
           M IYLTI FCLLIPIFLFLR SSKKQ LPPGSLGFP IGQSLSLLRAMRTNTAEQW QQR
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL GE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIR+HI+MHWH K+EVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 240
           LFGLEQGMRRE MIECFRVMI+GVWSIPINLPFTRYNQS+RASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMR+LA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLA 300

Query: 301 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYA VLQEQEEIAR+KKCGELLNWEDL KMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 420
           DIQFGGYLIPKGWQIFW SPVTH+DD IFREPSKFDP+RF+NPASIPPYCFLGFGSG RI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICPRQTFMA 477
           CPGNEFARVETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKI PRQTFMA
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTFMA 476

[Close]

BLAST of PI0009445 vs. ExPASy TrEMBL
Match: A0A6J1CC35 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 GN=LOC111010306 PE=3 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 7.1e-225
Identity = 390/480 (81.25%), Postives = 426/480 (88.75%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFL---RRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWV 60
           M IYLTI F  LIPIFLFL   RRS   Q+LPPGSLG P IGQSL LLRAMR+NTAEQW+
Sbjct: 1   MNIYLTI-FSFLIPIFLFLTRGRRSRSSQKLPPGSLGVPFIGQSLGLLRAMRSNTAEQWL 60

Query: 61  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 120
           Q+RI  YG ISKLTLFGKPTV +GGQ  NKLIF+GD A +SNQQNESLRAILGE NLLEL
Sbjct: 61  QRRITKYGPISKLTLFGKPTVLVGGQGANKLIFNGDTA-ISNQQNESLRAILGESNLLEL 120

Query: 121 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIV 180
            G+DHKRVR+ALV FLKPECLK+YVGKMDEE+R H+ MHW  K+EVTVLPLMKTLTFNIV
Sbjct: 121 TGDDHKRVRSALVXFLKPECLKQYVGKMDEEVRRHLEMHWQXKQEVTVLPLMKTLTFNIV 180

Query: 181 CSLLFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEK 240
           CSLLFGLE+G RRE MIECF+VMI GVWSIPINLPFTRYNQS++AS KIQEMLK LLDEK
Sbjct: 181 CSLLFGLEEGTRRERMIECFQVMIGGVWSIPINLPFTRYNQSRQASIKIQEMLKNLLDEK 240

Query: 241 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMR 300
           RVELE KG S HQDLITCLLSI+N+E+E VL+EKEI+HNI+LVMVAG+DTSSVLITFM+R
Sbjct: 241 RVELEVKGTSPHQDLITCLLSIKNKEHEQVLTEKEIIHNIILVMVAGYDTSSVLITFMVR 300

Query: 301 NLANNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRK 360
            LANNPTVYA VLQEQEEIAR+K+CGELL WEDL KMKYTWRVALETLRLVAP+FGGFRK
Sbjct: 301 FLANNPTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRK 360

Query: 361 AMNDIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSG 420
           AM DI+FGGYLIPKGWQIFWASP+TH+DDTIF EP KFDPNRFEN AS+P YCF+ FG G
Sbjct: 361 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGG 420

Query: 421 LRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICPRQTFMAL 478
            RICPG EFARVETLVTIHYLITQFTWKLLLDD FIRDPMP PTKG+PIK+CPR+T M L
Sbjct: 421 PRICPGYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETSMTL 478

[Close]

BLAST of PI0009445 vs. ExPASy TrEMBL
Match: A0A5J5A0L0 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_010117 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.2e-202
Identity = 333/473 (70.40%), Postives = 411/473 (86.89%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 60
           M I+ TIF  LL   FL +RR S  Q+LPPGSLG P+IGQSLSLL+AM+ NTAE+W+++R
Sbjct: 15  MNIFFTIFLFLLPIFFLLVRRRSSSQRLPPGSLGIPIIGQSLSLLQAMKANTAERWLEER 74

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IR YG+ISKL+LFGKPTVFI GQ+ NKL+F+GDC+V++NQQ +S+R ILG+RNLLEL GE
Sbjct: 75  IRKYGSISKLSLFGKPTVFIYGQAANKLVFTGDCSVITNQQAQSVRMILGDRNLLELSGE 134

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 180
           DHKRVR+ALVSFLKPECLK+YVGKM+EEIR H+ MHW  K++VTVLPLMKTLTFNI+CSL
Sbjct: 135 DHKRVRDALVSFLKPECLKQYVGKMEEEIREHLEMHWEGKQKVTVLPLMKTLTFNIICSL 194

Query: 181 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 240
           LFG+++G RR+ ++E F+ MI G+WS+P+NLPFTRYN+S RA+ ++Q MLK+L+ EKRVE
Sbjct: 195 LFGVQRGARRDKLVEYFQEMIQGMWSVPVNLPFTRYNRSLRATARVQNMLKDLIREKRVE 254

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 300
           LE++G SSHQDLITCLLSIR ++NE ++SEKEIVHN+MLVMVAG DTSSVLI+F+MR L+
Sbjct: 255 LEQQGASSHQDLITCLLSIRGKDNEELISEKEIVHNVMLVMVAGHDTSSVLISFIMRLLS 314

Query: 301 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           N+PTVYA VLQEQEEIA++K CGELL WEDL KMKYTWR A+ETLR+  PIFGGFRK + 
Sbjct: 315 NDPTVYAAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRAAMETLRMFPPIFGGFRKTLK 374

Query: 361 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 420
           DI++ GYLIPKGWQIFWAS +THMD +IF++PSKFDP RFEN AS+PPYCF+ FG G RI
Sbjct: 375 DIEYKGYLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRFENQASVPPYCFVAFGGGPRI 434

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFI-RDPMPTPTKGMPIKICPRQ 473
           CPG EFAR+ETLVT+HYL+T+FTWKL   D+F  RDPMP PT+G+PI+I P++
Sbjct: 435 CPGYEFARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPVPTEGLPIEIVPKK 487

[Close]

BLAST of PI0009445 vs. NCBI nr
Match: XP_004140824.1 (cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010272 [Cucumis sativus])

HSP 1 Score: 918.7 bits (2373), Expect = 2.1e-263
Identity = 454/478 (94.98%), Postives = 466/478 (97.49%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 60
           M IYLTIFFCLLIPIFLFLRRSSKK+QLPPGSLGFPLIGQSLSLLRAMRTNTAEQW QQR
Sbjct: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIR+HI MHWH K+EVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 240
           LFGLEQG RRE MIECFRVMI+GVWSIPINLPFTRYNQS+RASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLV+VAGFDTSSVLITFMMRNLA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300

Query: 301 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYA VLQEQEEIAR+KKCGELLNWEDL KMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 420
           DIQFGGYLIPKGWQIFW SPVTH+DDTIFREPSKFDP+RF+NPASIPPYCFLGFGSG RI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICPR-QTFMAL 478
           CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKI PR Q+FMA+
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 478

[Close]

BLAST of PI0009445 vs. NCBI nr
Match: XP_008457111.1 (PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa] >TYK14034.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 912.9 bits (2358), Expect = 1.1e-261
Identity = 449/476 (94.33%), Postives = 460/476 (96.64%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 60
           M IYLTI FCLLIPIFLFLR SSKKQ LPPGSLGFP IGQSLSLLRAMRTNTAEQW QQR
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL GE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIR+HI+MHWH K+EVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 240
           LFGLEQGMRRE MIECFRVMI+GVWSIPINLPFTRYNQS+RASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMR+LA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLA 300

Query: 301 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYA VLQEQEEIAR+KKCGELLNWEDL KMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 420
           DIQFGGYLIPKGWQIFW SPVTH+DD IFREPSKFDP+RF+NPASIPPYCFLGFGSG RI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICPRQTFMA 477
           CPGNEFARVETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKI PRQTFMA
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTFMA 476

[Close]

BLAST of PI0009445 vs. NCBI nr
Match: XP_038899130.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida])

HSP 1 Score: 829.3 bits (2141), Expect = 1.7e-236
Identity = 418/487 (85.83%), Postives = 440/487 (90.35%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFL---RRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWV 60
           M IYL   F LLIPIFLFL   R+SSK   LPPGSLGFP IGQSLSLLRAMR NTAE+W 
Sbjct: 1   MIIYLITTFSLLIPIFLFLLTGRKSSK--NLPPGSLGFPFIGQSLSLLRAMRINTAEEWA 60

Query: 61  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 120
           QQR+R YGAISKLTLFGKPTVF+GGQSG+KLIFSGDC  +SNQQNESLRAILGE NLLEL
Sbjct: 61  QQRMRKYGAISKLTLFGKPTVFVGGQSGHKLIFSGDCG-ISNQQNESLRAILGECNLLEL 120

Query: 121 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIV 180
           IGEDHKRVRNALVSFLKPECLK+YVGKMD  IR HI MHW  K++VTVLPLMKTLTFNIV
Sbjct: 121 IGEDHKRVRNALVSFLKPECLKQYVGKMD-XIRKHIKMHWQGKQQVTVLPLMKTLTFNIV 180

Query: 181 CSLLFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEK 240
           CSLLFGLEQGMRRE MIECFRVMI GVWSIPINLPFTRYNQS+RASR+IQEM+KELLDEK
Sbjct: 181 CSLLFGLEQGMRRERMIECFRVMIGGVWSIPINLPFTRYNQSRRASRRIQEMIKELLDEK 240

Query: 241 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMR 300
           RVELEEK  SSHQDLITCLLSI NEENEL+L+EKEIVHNIMLVMVAGFDTSSVLITF+MR
Sbjct: 241 RVELEEKRSSSHQDLITCLLSIGNEENELILTEKEIVHNIMLVMVAGFDTSSVLITFIMR 300

Query: 301 NLANNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRK 360
            LANNPTVY  VLQEQEEIAR+KKCGELL WEDL KMKYTWRVALETLRLVAPIFGGFRK
Sbjct: 301 FLANNPTVYVAVLQEQEEIARSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRK 360

Query: 361 AMNDIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFG-- 420
           AMNDI+FGGYLIPKGWQIFWASPVTH+D TIFREP KFDPNRF+NP SIPPYCF+GFG  
Sbjct: 361 AMNDIEFGGYLIPKGWQIFWASPVTHLDGTIFREPLKFDPNRFDNPTSIPPYCFVGFGGG 420

Query: 421 -----SGLRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICP 478
                 G RICPGNEFARVETLVTIHYLIT+FTWKLLLDDHFIRDPMPTPTKGMPIKICP
Sbjct: 421 PRICPGGPRICPGNEFARVETLVTIHYLITRFTWKLLLDDHFIRDPMPTPTKGMPIKICP 480

[Close]

BLAST of PI0009445 vs. NCBI nr
Match: XP_022139355.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia])

HSP 1 Score: 789.6 bits (2038), Expect = 1.5e-224
Identity = 390/480 (81.25%), Postives = 426/480 (88.75%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFL---RRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWV 60
           M IYLTI F  LIPIFLFL   RRS   Q+LPPGSLG P IGQSL LLRAMR+NTAEQW+
Sbjct: 1   MNIYLTI-FSFLIPIFLFLTRGRRSRSSQKLPPGSLGVPFIGQSLGLLRAMRSNTAEQWL 60

Query: 61  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 120
           Q+RI  YG ISKLTLFGKPTV +GGQ  NKLIF+GD A +SNQQNESLRAILGE NLLEL
Sbjct: 61  QRRITKYGPISKLTLFGKPTVLVGGQGANKLIFNGDTA-ISNQQNESLRAILGESNLLEL 120

Query: 121 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIV 180
            G+DHKRVR+ALV FLKPECLK+YVGKMDEE+R H+ MHW  K+EVTVLPLMKTLTFNIV
Sbjct: 121 TGDDHKRVRSALVXFLKPECLKQYVGKMDEEVRRHLEMHWQXKQEVTVLPLMKTLTFNIV 180

Query: 181 CSLLFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEK 240
           CSLLFGLE+G RRE MIECF+VMI GVWSIPINLPFTRYNQS++AS KIQEMLK LLDEK
Sbjct: 181 CSLLFGLEEGTRRERMIECFQVMIGGVWSIPINLPFTRYNQSRQASIKIQEMLKNLLDEK 240

Query: 241 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMR 300
           RVELE KG S HQDLITCLLSI+N+E+E VL+EKEI+HNI+LVMVAG+DTSSVLITFM+R
Sbjct: 241 RVELEVKGTSPHQDLITCLLSIKNKEHEQVLTEKEIIHNIILVMVAGYDTSSVLITFMVR 300

Query: 301 NLANNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRK 360
            LANNPTVYA VLQEQEEIAR+K+CGELL WEDL KMKYTWRVALETLRLVAP+FGGFRK
Sbjct: 301 FLANNPTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRK 360

Query: 361 AMNDIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSG 420
           AM DI+FGGYLIPKGWQIFWASP+TH+DDTIF EP KFDPNRFEN AS+P YCF+ FG G
Sbjct: 361 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGG 420

Query: 421 LRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKICPRQTFMAL 478
            RICPG EFARVETLVTIHYLITQFTWKLLLDD FIRDPMP PTKG+PIK+CPR+T M L
Sbjct: 421 PRICPGYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETSMTL 478

[Close]

BLAST of PI0009445 vs. NCBI nr
Match: KAA8523694.1 (hypothetical protein F0562_010117 [Nyssa sinensis])

HSP 1 Score: 714.9 bits (1844), Expect = 4.6e-202
Identity = 333/473 (70.40%), Postives = 411/473 (86.89%), Query Frame = 0


Query: 1   MTIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQR 60
           M I+ TIF  LL   FL +RR S  Q+LPPGSLG P+IGQSLSLL+AM+ NTAE+W+++R
Sbjct: 15  MNIFFTIFLFLLPIFFLLVRRRSSSQRLPPGSLGIPIIGQSLSLLQAMKANTAERWLEER 74

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IR YG+ISKL+LFGKPTVFI GQ+ NKL+F+GDC+V++NQQ +S+R ILG+RNLLEL GE
Sbjct: 75  IRKYGSISKLSLFGKPTVFIYGQAANKLVFTGDCSVITNQQAQSVRMILGDRNLLELSGE 134

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 180
           DHKRVR+ALVSFLKPECLK+YVGKM+EEIR H+ MHW  K++VTVLPLMKTLTFNI+CSL
Sbjct: 135 DHKRVRDALVSFLKPECLKQYVGKMEEEIREHLEMHWEGKQKVTVLPLMKTLTFNIICSL 194

Query: 181 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 240
           LFG+++G RR+ ++E F+ MI G+WS+P+NLPFTRYN+S RA+ ++Q MLK+L+ EKRVE
Sbjct: 195 LFGVQRGARRDKLVEYFQEMIQGMWSVPVNLPFTRYNRSLRATARVQNMLKDLIREKRVE 254

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 300
           LE++G SSHQDLITCLLSIR ++NE ++SEKEIVHN+MLVMVAG DTSSVLI+F+MR L+
Sbjct: 255 LEQQGASSHQDLITCLLSIRGKDNEELISEKEIVHNVMLVMVAGHDTSSVLISFIMRLLS 314

Query: 301 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           N+PTVYA VLQEQEEIA++K CGELL WEDL KMKYTWR A+ETLR+  PIFGGFRK + 
Sbjct: 315 NDPTVYAAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRAAMETLRMFPPIFGGFRKTLK 374

Query: 361 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 420
           DI++ GYLIPKGWQIFWAS +THMD +IF++PSKFDP RFEN AS+PPYCF+ FG G RI
Sbjct: 375 DIEYKGYLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRFENQASVPPYCFVAFGGGPRI 434

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFI-RDPMPTPTKGMPIKICPRQ 473
           CPG EFAR+ETLVT+HYL+T+FTWKL   D+F  RDPMP PT+G+PI+I P++
Sbjct: 435 CPGYEFARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPVPTEGLPIEIVPKK 487

[Close]

BLAST of PI0009445 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 338.2 bits (866), Expect = 1.1e-92
Identity = 177/481 (36.80%), Postives = 288/481 (59.88%), Query Frame = 0


Query: 1   MTIYLTIFF-CLLIPIFLFLRRSSKK---QQLPPGSLGFPLIGQSLSLLRAMRTNTAEQW 60
           M I + +F   +L+ + L LR+         LPPG+ G PLIG+S S L A R    E++
Sbjct: 3   MAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKF 62

Query: 61  VQQRIRNYGAIS----KLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGER 120
           +  R+R + + S    K  LFG PT  + G SGNK +F+ +  +V +   +S+  I    
Sbjct: 63  ITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIF-PS 122

Query: 121 NLLELIGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTL 180
           ++     E+ +++R  L  F+KPE L+ YVG MDE  + H    W ++++V V PL K  
Sbjct: 123 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKF 182

Query: 181 TFNIVCSLLFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKE 240
           TF+I C     +E   R   + E F  +  G++SIPI+LP TR+N++ +ASR +++ +  
Sbjct: 183 TFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSA 242

Query: 241 LLDEKRVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLI 300
           ++ +++ EL+        D+++ +L    E  +  L++K     I+ +++ G DT+S++ 
Sbjct: 243 IVRQRKEELKAGKALEEHDILSHMLMNIGETKDEDLADK-----IIGLLIGGHDTASIVC 302

Query: 301 TFMMRNLANNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIF 360
           TF++  LA  P VY  VLQEQ+EI + KK  E L WED+ KM+Y+W VA E +R+V P+ 
Sbjct: 303 TFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLS 362

Query: 361 GGFRKAMNDIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFL 420
           G FR+A++   F G+ IPKGW+++W++  THM+   F EP +F+PNRFE  +   PY ++
Sbjct: 363 GTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEG-SGPKPYTYV 422

Query: 421 GFGSGLRICPGNEFARVETLVTIHYLITQFTWKLLL--DDHFIRDPMPTPTKGMPIKICP 472
            FG G R+CPG E+AR+E L+ +H L+ +F W+ +   ++  + DP+P P KG+PI+I P
Sbjct: 423 PFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFP 476

[Close]

BLAST of PI0009445 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 316.6 bits (810), Expect = 3.4e-86
Identity = 169/472 (35.81%), Postives = 278/472 (58.90%), Query Frame = 0


Query: 2   TIYLTIFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTA-EQWVQQR 61
           TI  T+FF          ++  KK++L PG +G P IG+++   +A ++N   E +V  R
Sbjct: 19  TISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPR 78

Query: 62  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 121
           I  +G I K  + G PT+ + G   N+LI S + ++V +    S   ++G   ++   GE
Sbjct: 79  IIKHGNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGE 138

Query: 122 DHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSL 181
            H+ +R  + + L    L+  + K+ + ++ H    W  KEE+++    K LTF +V   
Sbjct: 139 KHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFEC 198

Query: 182 LFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVE 241
           L+G++  +   GM+E F  ++ GV+++P+  P +++ ++++A  +I+  L   + EKR E
Sbjct: 199 LYGIKVEI---GMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRRE 258

Query: 242 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLA 301
           +E++G  + +   T    +  E  + V++E+E+V N++L++ A  DT+S  ++   + LA
Sbjct: 259 MEKEG--AEKPNTTLFSRLVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLA 318

Query: 302 NNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMN 361
            +PT   T+LQE  +I  NK  GE L  ED+ KMKY+W+V  ET+RL  PIFG FRKA+ 
Sbjct: 319 QHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVA 378

Query: 362 DIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRI 421
           DI +GGY IPKGW+I W +  TH +  IF++P  FDP RF+ P  I  Y +L FG G R+
Sbjct: 379 DIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKP--IQAYTYLPFGGGPRL 438

Query: 422 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFI-RDPMPTPTKGMPIKICPR 472
           C G++ A++  LV +H+++T F W L+  D  I  DP+P P+ GMPIKI P+
Sbjct: 439 CAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483

[Close]

BLAST of PI0009445 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 254.6 bits (649), Expect = 1.6e-67
Identity = 148/463 (31.97%), Postives = 250/463 (54.00%), Query Frame = 0


Query: 7   IFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQRIRNYGA 66
           +F CLL  I    R SS K  LPPG++G+P +G++  L     +     +   + R YG+
Sbjct: 15  LFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLY----SQDPNVFFAAKQRRYGS 74

Query: 67  ISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVR 126
           + K  + G P V I      K +      +       S   +LG++ +    G+ H ++R
Sbjct: 75  VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLR 134

Query: 127 NALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSLLFGLEQ 186
             ++    P+ ++  V  ++   +  +N  W    ++     MKT TFN+    + G ++
Sbjct: 135 KLVLRAFMPDAIRNMVPHIESIAQESLN-SWDG-TQLNTYQEMKTYTFNVALISILGKDE 194

Query: 187 GMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVELEEKGG 246
              RE +  C+ ++  G  S+PINLP T ++++ +A +++ ++L  +L ++R     +  
Sbjct: 195 VYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR-----QNP 254

Query: 247 SSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLANNPTVY 306
           SSH DL+   +     E++  L++++I  NI+ V+ A  DT++ ++T++++ LA+NPTV 
Sbjct: 255 SSHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVL 314

Query: 307 ATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGG 366
             V +EQ  I ++KK GE L WED  KM  T+RV  ETLR    +   FR+A+ D+++ G
Sbjct: 315 EAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEG 374

Query: 367 YLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRICPGNEF 426
           YLIPKGW++       H +  IF +P KFDP+RFE   +  P  F+ FGSG+  CPGNE 
Sbjct: 375 YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE--VAPKPNTFMPFGSGIHSCPGNEL 434

Query: 427 ARVETLVTIHYLITQFTWKLL-LDDHFIRDPMPTPTKGMPIKI 469
           A++E  V IH+L T++ W ++   D     P   P  G+PI +
Sbjct: 435 AKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459

[Close]

BLAST of PI0009445 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 245.4 bits (625), Expect = 9.5e-65
Identity = 147/470 (31.28%), Postives = 248/470 (52.77%), Query Frame = 0


Query: 3   IYLTIFFCLLIPIFLFL-----RRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWV 62
           ++LT+F   L   FL       R  S K  LPPG++G+P +G++  L     +     + 
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY----SQDPNVFF 65

Query: 63  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 122
           Q + + YG++ K  + G P V I      K +      +       S   +LG++ +   
Sbjct: 66  QSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFH 125

Query: 123 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIV 182
            G+ H ++R  ++    PE ++  V   D E  A  ++       +     MKT TFN+ 
Sbjct: 126 QGDYHAKLRKLVLRAFMPESIRNMV--PDIESIAQDSLRSWEGTMINTYQEMKTYTFNVA 185

Query: 183 CSLLFGLEQGMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEK 242
              +FG ++ + RE +  C+ ++  G  S+P+NLP T +++S +A +++ ++L  +L E+
Sbjct: 186 LLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSER 245

Query: 243 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMR 302
           R     + GSSH DL+   +  + E     L++++I  NI+ V+ A  DT++ +++++++
Sbjct: 246 R-----QNGSSHNDLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILK 305

Query: 303 NLANNPTVYATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRK 362
            LA NP V   V +EQ  I ++K+ GE L W D  KM  T RV  ETLR+ + +   FR+
Sbjct: 306 YLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFRE 365

Query: 363 AMNDIQFGGYLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSG 422
           A+ D+++ GYLIPKGW++       H    IF  P KFDP+RFE   +  P  F+ FG+G
Sbjct: 366 AVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFE--VAPKPNTFMPFGNG 425

Query: 423 LRICPGNEFARVETLVTIHYLITQFTWKLL-LDDHFIRDPMPTPTKGMPI 467
              CPGNE A++E  + IH+L T+++W ++   D     P   P  G+PI
Sbjct: 426 THSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPI 457

[Close]

BLAST of PI0009445 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-64
Identity = 143/445 (32.13%), Postives = 243/445 (54.61%), Query Frame = 0


Query: 7   IFFCLLIPIFLFLRRSSKKQQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWVQQRIRNYGA 66
           +F CLL  I    R SS K  LPPG++G+P +G++  L     +     +   + R YG+
Sbjct: 15  LFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLY----SQDPNVFFAAKQRRYGS 74

Query: 67  ISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVR 126
           + K  + G P V I      K +      +       S   +LG++ +    G+ H ++R
Sbjct: 75  VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLR 134

Query: 127 NALVSFLKPECLKEYVGKMDEEIRAHINMHWHSKEEVTVLPLMKTLTFNIVCSLLFGLEQ 186
             ++    P+ ++  V  ++   +  +N  W    ++     MKT TFN+    + G ++
Sbjct: 135 KLVLRAFMPDAIRNMVPHIESIAQESLN-SWDG-TQLNTYQEMKTYTFNVALISILGKDE 194

Query: 187 GMRREGMIECFRVMITGVWSIPINLPFTRYNQSQRASRKIQEMLKELLDEKRVELEEKGG 246
              RE +  C+ ++  G  S+PINLP T ++++ +A +++ ++L  +L ++R     +  
Sbjct: 195 VYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR-----QNP 254

Query: 247 SSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRNLANNPTVY 306
           SSH DL+   +     E++  L++++I  NI+ V+ A  DT++ ++T++++ LA+NPTV 
Sbjct: 255 SSHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVL 314

Query: 307 ATVLQEQEEIARNKKCGELLNWEDLTKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGG 366
             V +EQ  I ++KK GE L WED  KM  T+RV  ETLR    +   FR+A+ D+++ G
Sbjct: 315 EAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEG 374

Query: 367 YLIPKGWQIFWASPVTHMDDTIFREPSKFDPNRFENPASIPPYCFLGFGSGLRICPGNEF 426
           YLIPKGW++       H +  IF +P KFDP+RFE   +  P  F+ FGSG+  CPGNE 
Sbjct: 375 YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE--VAPKPNTFMPFGSGIHSCPGNEL 434

Query: 427 ARVETLVTIHYLITQFTWKLLLDDH 452
           A++E  V IH+L T++    L +D+
Sbjct: 435 AKLEISVLIHHLTTKYRLVHLQNDN 441

The following BLAST results are available for this feature:

BLAST of PI0009445 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50EK1	1.8e-100	40.34	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q50EK0	8.9e-100	40.08	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]
A0A0U2U8U5	1.0e-95	37.84	Beta-amyrin 28-monooxygenase OS=Barbarea vulgaris OX=50459 GN=CYP716A81 PE=1 SV=...	[more]
Q6WG30	6.6e-95	37.34	Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2	[more]
Q9AXM6	5.6e-94	37.53	Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1	[more]

BLAST of PI0009445 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA02	1.0e-263	94.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1	[more]
A0A5A7TZG7	5.5e-262	94.33	Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C4V0	5.5e-262	94.33	cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1	[more]
A0A6J1CC35	7.1e-225	81.25	LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 G...	[more]
A0A5J5A0L0	2.2e-202	70.40	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_010117 PE=3 SV=1	[more]

BLAST of PI0009445 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140824.1	2.1e-263	94.98	cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010...	[more]
XP_008457111.1	1.1e-261	94.33	PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P4...	[more]
XP_038899130.1	1.7e-236	85.83	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida]	[more]
XP_022139355.1	1.5e-224	81.25	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia]	[more]
KAA8523694.1	4.6e-202	70.40	hypothetical protein F0562_010117 [Nyssa sinensis]	[more]

BLAST of PI0009445 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36110.1	1.1e-92	36.80	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G42850.1	3.4e-86	35.81	cytochrome P450, family 718	[more]
AT5G45340.1	1.6e-67	31.97	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	9.5e-65	31.28	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT5G45340.2	1.6e-64	32.13	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 216..236
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 8..449 e-value: 5.2E-10 score: 36.6
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 5..471
None	No IPR available	PANTHER	PTHR24286:SF237	OS07G0520300 PROTEIN	coord: 5..471
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 339..350 score: 34.88 coord: 421..432 score: 38.15 coord: 283..300 score: 30.91 coord: 412..421 score: 51.6
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 29..447 e-value: 2.5E-65 score: 221.0
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 172..190 score: 22.53 coord: 378..402 score: 27.32 coord: 411..421 score: 50.3 coord: 292..318 score: 18.82 coord: 61..80 score: 25.21 coord: 272..289 score: 26.26 coord: 421..444 score: 27.13
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 19..470 e-value: 1.1E-114 score: 385.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 21..471
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 414..423

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0009445.1	PI0009445.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen