PI0009416 (gene) Melon (PI 482460) v1

Overview
NamePI0009416
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionULP_PROTEASE domain-containing protein
Locationchr12: 17145577 .. 17146067 (+)
RNA-Seq ExpressionPI0009416
SyntenyPI0009416
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAGGAGAAAGTGAAACTGAAAGAGGTGAAGAAGGAGGAAGAGAACTGGAATTTGAAGATGAAGGAGGAGGAGAATGAACCTGAAGGGAAACAAGAAGAAACCAAGTTGGAGGTGATCAATGTAACCAGCAATGAAGTAGACTTGAACATCTTCAAGGTAAGTGACTCACCAATAAACTAAAATGTCTAATATTTACGTCTTATTGAATGTGGATGATGTTTTTGAATTAGGAGAAGGTCTCAACTAAAATGTCGAAGGAAAAGGAAGTTGTCTGTGAGTCGACATCGGTCTTGCCAATAGCTTTGAAGTCGATTCTTAGATATGTTGAGAAAGTGATGGATAAAGAATCCAGTATTACTATTCAAGTACCTATTGGGATTTTTGGTATCAGTCGCAAGAGTTTTGTTCTCCGAAAGGACAAAATTGACTTATGTAGCATGAACCAAGTGAAAACGTTTACAGTGGTGGTGGCCTATATGACATAA

mRNA sequence

ATGGAGGAGGAGAAAGTGAAACTGAAAGAGGTGAAGAAGGAGGAAGAGAACTGGAATTTGAAGATGAAGGAGGAGGAGAATGAACCTGAAGGGAAACAAGAAGAAACCAAGTTGGAGGTGATCAATGTAACCAGCAATGAAGTAGACTTGAACATCTTCAAGGAGAAGGTCTCAACTAAAATGTCGAAGGAAAAGGAAGTTGTCTGTGAGTCGACATCGGTCTTGCCAATAGCTTTGAAGTCGATTCTTAGATATGTTGAGAAAGTGATGGATAAAGAATCCAGTATTACTATTCAAGTACCTATTGGGATTTTTGGTATCAGTCGCAAGAGTTTTGTTCTCCGAAAGGACAAAATTGACTTATGTAGCATGAACCAAGTGAAAACGTTTACAGTGGTGGTGGCCTATATGACATAA

Coding sequence (CDS)

ATGGAGGAGGAGAAAGTGAAACTGAAAGAGGTGAAGAAGGAGGAAGAGAACTGGAATTTGAAGATGAAGGAGGAGGAGAATGAACCTGAAGGGAAACAAGAAGAAACCAAGTTGGAGGTGATCAATGTAACCAGCAATGAAGTAGACTTGAACATCTTCAAGGAGAAGGTCTCAACTAAAATGTCGAAGGAAAAGGAAGTTGTCTGTGAGTCGACATCGGTCTTGCCAATAGCTTTGAAGTCGATTCTTAGATATGTTGAGAAAGTGATGGATAAAGAATCCAGTATTACTATTCAAGTACCTATTGGGATTTTTGGTATCAGTCGCAAGAGTTTTGTTCTCCGAAAGGACAAAATTGACTTATGTAGCATGAACCAAGTGAAAACGTTTACAGTGGTGGTGGCCTATATGACATAA

Protein sequence

MEEEKVKLKEVKKEEENWNLKMKEEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEVVCESTSVLPIALKSILRYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQVKTFTVVVAYMT
Homology
BLAST of PI0009416 vs. ExPASy TrEMBL
Match: A0A5A7SP06 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G003280 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.6e-23
Identity = 60/99 (60.61%), Postives = 80/99 (80.81%), Query Frame = 0

Query: 39  EVINVTSNEVDLNIFKEKVSTKMSKEKEVVCESTSVLPIALKSILRYVEKVMDKESSITI 98
           +V++  +NE + +IFK++   KM  E+EVVCESTS LP+ALKSILRY EKVM+K+SS T 
Sbjct: 157 QVVHKITNEPEFDIFKKRGLIKMPTEREVVCESTSTLPLALKSILRYAEKVMEKDSSTTF 216

Query: 99  QVPIGIFGISRKSFVLRKDKIDLCSMNQVKTFTVVVAYM 138
            +P  IFGISRKS+VLR+D ID+C+MN+V+TFT+VV  M
Sbjct: 217 PLPADIFGISRKSYVLREDIIDVCNMNEVRTFTLVVYMM 255

BLAST of PI0009416 vs. ExPASy TrEMBL
Match: A0A5A7V2Z2 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold39G00510 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 7.7e-23
Identity = 67/114 (58.77%), Postives = 91/114 (79.82%), Query Frame = 0

Query: 24  EEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEVVCESTSVLPIALKSIL 83
           E  ++P+ K+++ K EV+ +  NE +LN+ KE+   KM  EKEVVCESTS LP+ALKSIL
Sbjct: 350 ESLDKPKHKEKKGK-EVMKM--NEPELNVLKERDLIKMPIEKEVVCESTSTLPLALKSIL 409

Query: 84  RYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQVKTFTVVVAYM 138
           RY +KVM+K+SSIT  +P  +FG+SRK+FVLR+D +DLC+MN+VKTFT +VAYM
Sbjct: 410 RYAKKVMEKDSSITFSLPADLFGVSRKTFVLREDILDLCNMNEVKTFT-LVAYM 459

BLAST of PI0009416 vs. ExPASy TrEMBL
Match: A0A5A7VQR7 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold50G00060 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 1.0e-22
Identity = 75/130 (57.69%), Postives = 94/130 (72.31%), Query Frame = 0

Query: 12  KKEEENWNLKM----KEEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEV 71
           K EE    +K+    K +ENE +GK      EV+ +  NE +L++ KE+   KM  EKEV
Sbjct: 305 KIEENGKRIKVESLDKPKENEKKGK------EVMKM--NEPELDVLKERDLIKMPTEKEV 364

Query: 72  VCESTSVLPIALKSILRYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQV 131
           VCE TS LP+ALKSILRY EKVM+K+SSIT  +P  +FGISRKSFVLR+D IDLC+MN+V
Sbjct: 365 VCELTSTLPLALKSILRYAEKVMEKDSSITFSLPADLFGISRKSFVLREDIIDLCNMNEV 424

Query: 132 KTFTVVVAYM 138
           KTFT +VAYM
Sbjct: 425 KTFT-LVAYM 425

BLAST of PI0009416 vs. ExPASy TrEMBL
Match: A0A5A7TSB6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G006140 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 1.0e-22
Identity = 68/114 (59.65%), Postives = 90/114 (78.95%), Query Frame = 0

Query: 24  EEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEVVCESTSVLPIALKSIL 83
           E  N+P+ K+++ K EV+ +  NE++L++ KE+   KM  EKEVVCESTS L +ALKSIL
Sbjct: 2   ESLNKPKQKEKKGK-EVMKM--NELELDVLKERDLIKMHTEKEVVCESTSTLSLALKSIL 61

Query: 84  RYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQVKTFTVVVAYM 138
           RY EKVM+K+SSIT  +P  +FGISRK+ VLRKD +DLC+MN+VKTFT +VAYM
Sbjct: 62  RYTEKVMEKDSSITFSLPADLFGISRKTSVLRKDIVDLCNMNEVKTFT-LVAYM 111

BLAST of PI0009416 vs. ExPASy TrEMBL
Match: A0A5D3DGP6 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00560 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 1.0e-22
Identity = 75/130 (57.69%), Postives = 94/130 (72.31%), Query Frame = 0

Query: 12  KKEEENWNLKM----KEEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEV 71
           K EE    +K+    K +ENE +GK      EV+ +  NE +L++ KE+   KM  EKEV
Sbjct: 319 KIEENGKRIKVESLDKPKENEKKGK------EVMKM--NEPELDVLKERDLIKMPTEKEV 378

Query: 72  VCESTSVLPIALKSILRYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQV 131
           VCE TS LP+ALKSILRY EKVM+K+SSIT  +P  +FGISRKSFVLR+D IDLC+MN+V
Sbjct: 379 VCELTSTLPLALKSILRYAEKVMEKDSSITFSLPADLFGISRKSFVLREDIIDLCNMNEV 438

Query: 132 KTFTVVVAYM 138
           KTFT +VAYM
Sbjct: 439 KTFT-LVAYM 439

BLAST of PI0009416 vs. NCBI nr
Match: XP_031739719.1 (uncharacterized protein LOC116403244 [Cucumis sativus] >XP_031745727.1 uncharacterized protein LOC105434907 [Cucumis sativus])

HSP 1 Score: 129.4 bits (324), Expect = 2.4e-26
Identity = 73/119 (61.34%), Postives = 98/119 (82.35%), Query Frame = 0

Query: 20  LKMKEEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEVVCESTSVLPIAL 79
           +K+ + +++   K E  ++E INV  ++V+L+IFKE+   KM+KEKEVVCESTSVLP+ +
Sbjct: 1   MKIIKGKSKLMDKIEGKEVEEINVNDDKVELDIFKERALIKMTKEKEVVCESTSVLPMGM 60

Query: 80  KSILRYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQVKTFTVVVAYMT 139
           K IL YVEK+M+K+SSITI +PIGIFGISRK+ VLR+D IDLC+MN+VKTFT +VAYMT
Sbjct: 61  KLILIYVEKIMEKDSSITIPLPIGIFGISRKTSVLREDIIDLCNMNEVKTFT-LVAYMT 118

BLAST of PI0009416 vs. NCBI nr
Match: XP_031745418.1 (uncharacterized protein LOC116405627 [Cucumis sativus])

HSP 1 Score: 129.0 bits (323), Expect = 3.1e-26
Identity = 72/119 (60.50%), Postives = 98/119 (82.35%), Query Frame = 0

Query: 20  LKMKEEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEVVCESTSVLPIAL 79
           +K+ + +++   K E  ++E INV  ++V+L+IFKE+   KM+KEKEVVCESTSVLP+ +
Sbjct: 1   MKIIKGKSKLMDKIEGKEVEEINVNDDKVELDIFKERALIKMTKEKEVVCESTSVLPMGM 60

Query: 80  KSILRYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQVKTFTVVVAYMT 139
           K IL YVEK+M+K+SSITI +P+GIFGISRK+ VLR+D IDLC+MN+VKTFT +VAYMT
Sbjct: 61  KLILIYVEKIMEKDSSITIPLPVGIFGISRKTSVLREDIIDLCNMNEVKTFT-LVAYMT 118

BLAST of PI0009416 vs. NCBI nr
Match: KAA0026313.1 (putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa])

HSP 1 Score: 118.2 bits (295), Expect = 5.5e-23
Identity = 60/99 (60.61%), Postives = 80/99 (80.81%), Query Frame = 0

Query: 39  EVINVTSNEVDLNIFKEKVSTKMSKEKEVVCESTSVLPIALKSILRYVEKVMDKESSITI 98
           +V++  +NE + +IFK++   KM  E+EVVCESTS LP+ALKSILRY EKVM+K+SS T 
Sbjct: 157 QVVHKITNEPEFDIFKKRGLIKMPTEREVVCESTSTLPLALKSILRYAEKVMEKDSSTTF 216

Query: 99  QVPIGIFGISRKSFVLRKDKIDLCSMNQVKTFTVVVAYM 138
            +P  IFGISRKS+VLR+D ID+C+MN+V+TFT+VV  M
Sbjct: 217 PLPADIFGISRKSYVLREDIIDVCNMNEVRTFTLVVYMM 255

BLAST of PI0009416 vs. NCBI nr
Match: KAA0060131.1 (putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa])

HSP 1 Score: 116.7 bits (291), Expect = 1.6e-22
Identity = 67/114 (58.77%), Postives = 91/114 (79.82%), Query Frame = 0

Query: 24  EEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEVVCESTSVLPIALKSIL 83
           E  ++P+ K+++ K EV+ +  NE +LN+ KE+   KM  EKEVVCESTS LP+ALKSIL
Sbjct: 350 ESLDKPKHKEKKGK-EVMKM--NEPELNVLKERDLIKMPIEKEVVCESTSTLPLALKSIL 409

Query: 84  RYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQVKTFTVVVAYM 138
           RY +KVM+K+SSIT  +P  +FG+SRK+FVLR+D +DLC+MN+VKTFT +VAYM
Sbjct: 410 RYAKKVMEKDSSITFSLPADLFGVSRKTFVLREDILDLCNMNEVKTFT-LVAYM 459

BLAST of PI0009416 vs. NCBI nr
Match: TYK22449.1 (putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa])

HSP 1 Score: 116.3 bits (290), Expect = 2.1e-22
Identity = 75/130 (57.69%), Postives = 94/130 (72.31%), Query Frame = 0

Query: 12  KKEEENWNLKM----KEEENEPEGKQEETKLEVINVTSNEVDLNIFKEKVSTKMSKEKEV 71
           K EE    +K+    K +ENE +GK      EV+ +  NE +L++ KE+   KM  EKEV
Sbjct: 319 KIEENGKRIKVESLDKPKENEKKGK------EVMKM--NEPELDVLKERDLIKMPTEKEV 378

Query: 72  VCESTSVLPIALKSILRYVEKVMDKESSITIQVPIGIFGISRKSFVLRKDKIDLCSMNQV 131
           VCE TS LP+ALKSILRY EKVM+K+SSIT  +P  +FGISRKSFVLR+D IDLC+MN+V
Sbjct: 379 VCELTSTLPLALKSILRYAEKVMEKDSSITFSLPADLFGISRKSFVLREDIIDLCNMNEV 438

Query: 132 KTFTVVVAYM 138
           KTFT +VAYM
Sbjct: 439 KTFT-LVAYM 439

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7SP062.6e-2360.61Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7V2Z27.7e-2358.77Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7VQR71.0e-2257.69Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7TSB61.0e-2259.65Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3DGP61.0e-2257.69Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
XP_031739719.12.4e-2661.34uncharacterized protein LOC116403244 [Cucumis sativus] >XP_031745727.1 uncharact... [more]
XP_031745418.13.1e-2660.50uncharacterized protein LOC116405627 [Cucumis sativus][more]
KAA0026313.15.5e-2360.61putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa][more]
KAA0060131.11.6e-2258.77putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa][more]
TYK22449.12.1e-2257.69putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1..28

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009416.1PI0009416.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0016301 kinase activity