PI0009344 (gene) Melon (PI 482460) v1

Overview
NamePI0009344
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionprotein KINESIN LIGHT CHAIN-RELATED 1
Locationchr01: 23058044 .. 23061534 (+)
RNA-Seq ExpressionPI0009344
SyntenyPI0009344
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAAAAGGGTTTTGCAGAGGTTTACGCTGCAAATCCGCAGCCGACTGTTCAATTTCAATTACACAATGAGAAGAAACGCTTCAATTTCCATTCTTTCTCACTCCAATCGTCTCAATCTCCGCTTACTTTCATCCCTCATTTCCCCAAATTCGATTTCCAATCCACCTTCAAATTCCTCCCCTGTTTCAACTCCCAATCTCAAGCCCTGCACCAAAAATAATGGATTGTTCTTCAAGTTCACTCGATTTCAAGCAAACCCATCTCGAAATTTGGATACCCTTGTGGAGAAGCGGCCTGAAATTTCCTCAAGACAGCGAAAACTCATGGAAAAATCCGAGCTTGAGGAGGTTTTTGAGTCTGCTGACACTGTTGATGAAATGCTCAAGGCGTTTAAAGACATGGAATCCGCTTTTGAAGAGAAGGATCTCGGTTTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAACAAGGTGAGGATCCTGAACAGATTTTGTTGTATGCTAATAGAGCTTTAAAAGCTTTGGATAAAGATGATGATAGAAATCCTTCTTTACCTGTTGCTATGACTTTACAATTGATTGGTTCTGTTAATTATAGTTTGAAGAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCCAATAGGATTCTGGGTCAGTTAGAGAAGAAGGGTTTTAGTGCTGAGGATCTTAGGCCAGTACTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAAACTGCAATGGGGAGAAGAGAAGAAGCGCTTTCTAATCTCAGGAAGTCTTTAGATATTAAGGAGTTGATGTTGGAAAAGGATAGTAGAGAATTAGGGACGGCGAATCGTGATTTGGCCGAAGCTTATGTTGCAATATTGAACTTTAAGGATGCTCTTCCCTTTTGTATGAAGGGATTGGAGATACATAAGAAGCAGTTGGGCAATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTTATTTATAGTGGCTTGGAAGAACATCAAAAGGCATTGGAGCAGAATGAATTGTCTCGGAGGGTTTTGAAGAACTGGGGCCTTAATTCTGATTTGCTTCGTGCTGAAATCGATGCTGCTAATATGCAAATTTCACTCGGTAGGTACGATGAGGCCATTAGTACTCTTAAGGAAGTTGTTCAGCATACAGATAAAGACAGTGAAATTCGGGGAGTTGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTGCTGATGCAAAGAGATGTTTGGCAATTGCTAGTGGAATTTTCGACAAGAGAGAAGGAACTTCTCCTGTGGAAGCTGCTGAGGCGTTTTCGGAGATATCAATGCAGTATGAGACAATGAATGACTTTGAAACTGCAATTTCTTTATTAAAAAGATCTCTAGCTTTGCTTGAGAAGCTTCCACAAGAGCAGCACTCGGAGGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTGCTGTTGACTGGTAAGGTTCAAGAAGCTATCCCATACTTGGAGGGTGCAGCAGAGACTTTAAAAGAGAGTTTTGGGTCGAAGCATTTTGGAGTTGGATATATATACAATAATCTTGGAGCTGCGTATTTGGAACTTGACAGACCTCAATCAGCTGCGCAAATGTTTGAAGTGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCCTGTCACAATGTTTCAAAGGCATATGGTGCAATGGGAAGGTATGGTTACTTTCAAGCGTCACTTTTTCTCTCCATTGCTGGTAACATAGTTACATTCTTTACAACGTACAATGTTTTACTTATTATTATTCATTGAAATATCCATTGAGGGAGTAGGGTTTTATTGATGATATTGAGACACAGAACCATATGGATTGAGATCAGAATGTTTTGTTTTGAATGGCAGAGTAGGAAATTGATCCATATAATATGTCTCTGATCTCAAAACTGTTCATTTCCTTTTGGAGATATATTTAACTTCATCATGTACGATAAATTCACTTGCCATTGTTCTTAAACCAAATGTGGCATGTTTAAATACTTGCCCCCTTCTGCTTAGGTTAACTGTACTTTTACGGTTGTGGAAGAACTAGTGGTTAATTGGATGCACAAAAATGTCAAGGAGTCCTTCATGGGGGGCTCTTACCAATTATTGGATGACCCAAACTAATGGGTTCATCTTGGACTGGAATTTCCTTTGAGTCACTTGACAGGAATGATAAAGTATCACTAGTATTTAGAATAGGAATATTACAGGGCACAGAGCTTTTGCCGATCTTGTTAAAAAAGTGAGTATGGTGAATATTGATAAGTTTATATTCTATAGCTATCCTTGTATGGAAATATCTAAGTTGTGTTCGGAGATGAATGCTTGTGGACCTAACTTGATACTGCAAGTTCGATGTATGGGCTGAGTTGATTTTGAAATTTGATCACTTTGTACTTTTTAACTATCAATCTGTCCTGAATGTATTCGTATTAAACACTATGTCCAGTACAGTCCTGCATCTTTCTTATTGCCCTAGGTATCTTTATCATTTGTTATTGAATGATGCCAAACCTGCAGAAAACTCTTCTTTTCTATCAACATTCGTAATGAAAACCTTTGTTTCTGGATCCATGGATCACAGCAGCAATTTGGCTAGTGATGGCCATTCATGAACTCATTACTTTACTAATCTTTTCTGTGTGTTGGTGCAGTTATGCCCTTGCAATTGAATTCCAGGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCGACTGCCGAAGACGAACTTAGAGAAGCCCGACGAACGCTAGAACAATTGAAGAGGAAAGCTCAAGGTACATCCAAATCCGAGCTCTCAACAAAGGCCTTGCCCCTACCTCATTCACCAACGTCTTCCAAAACTCTGCAGCCTGATCTCCCTGCCCGTCAGAAACATCAATAACAACAAGATCTGATAATATGCTATTTTATGACTTAAGATAAATCGATGGATCATACCACACTCGTTGGTAAGTTTTATCCTAAAAGAACTGATATTTTTAGGGAAAATATTTTCCTTTAAAAAGTTGGTCATCTTGTGCATCGCTGGAATATCCTTGAAAAATATACAAGACTCCCAAGTTTTGCTTTCATGTCTATATATATATCCTGCTGGGAAACATATTCATTGGAAGCTATATGCTTGGGAAAGAGACGGCTGCAGTAGCTCGTAGAGTTATCGAATCTAACGGCTGCGTTTTCTGAGAACTTTGCATCAACTCATCTTGAAAAAGTTCATAGATGTTTATTTAGACTGGTCTTTCAGAATTACATGTATGTAAATGAGTTTGTAATTTTGGTTTTAGTTGCAAAACTCTAATGTAAGAAAGAAAGTTTCTGGGTTGCCTTGAGTTTGGTTTTAGAAAGAAACACATCTCATTTTGTTCTTGTGTTTTTTCTAAGTTTATTACTATTTCAAGTGGCTTTGATTTGGTTGAAAAA

mRNA sequence

CTCAAAAGGGTTTTGCAGAGGTTTACGCTGCAAATCCGCAGCCGACTGTTCAATTTCAATTACACAATGAGAAGAAACGCTTCAATTTCCATTCTTTCTCACTCCAATCGTCTCAATCTCCGCTTACTTTCATCCCTCATTTCCCCAAATTCGATTTCCAATCCACCTTCAAATTCCTCCCCTGTTTCAACTCCCAATCTCAAGCCCTGCACCAAAAATAATGGATTGTTCTTCAAGTTCACTCGATTTCAAGCAAACCCATCTCGAAATTTGGATACCCTTGTGGAGAAGCGGCCTGAAATTTCCTCAAGACAGCGAAAACTCATGGAAAAATCCGAGCTTGAGGAGGTTTTTGAGTCTGCTGACACTGTTGATGAAATGCTCAAGGCGTTTAAAGACATGGAATCCGCTTTTGAAGAGAAGGATCTCGGTTTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAACAAGGTGAGGATCCTGAACAGATTTTGTTGTATGCTAATAGAGCTTTAAAAGCTTTGGATAAAGATGATGATAGAAATCCTTCTTTACCTGTTGCTATGACTTTACAATTGATTGGTTCTGTTAATTATAGTTTGAAGAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCCAATAGGATTCTGGGTCAGTTAGAGAAGAAGGGTTTTAGTGCTGAGGATCTTAGGCCAGTACTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAAACTGCAATGGGGAGAAGAGAAGAAGCGCTTTCTAATCTCAGGAAGTCTTTAGATATTAAGGAGTTGATGTTGGAAAAGGATAGTAGAGAATTAGGGACGGCGAATCGTGATTTGGCCGAAGCTTATGTTGCAATATTGAACTTTAAGGATGCTCTTCCCTTTTGTATGAAGGGATTGGAGATACATAAGAAGCAGTTGGGCAATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTTATTTATAGTGGCTTGGAAGAACATCAAAAGGCATTGGAGCAGAATGAATTGTCTCGGAGGGTTTTGAAGAACTGGGGCCTTAATTCTGATTTGCTTCGTGCTGAAATCGATGCTGCTAATATGCAAATTTCACTCGGTAGGTACGATGAGGCCATTAGTACTCTTAAGGAAGTTGTTCAGCATACAGATAAAGACAGTGAAATTCGGGGAGTTGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTGCTGATGCAAAGAGATGTTTGGCAATTGCTAGTGGAATTTTCGACAAGAGAGAAGGAACTTCTCCTGTGGAAGCTGCTGAGGCGTTTTCGGAGATATCAATGCAGTATGAGACAATGAATGACTTTGAAACTGCAATTTCTTTATTAAAAAGATCTCTAGCTTTGCTTGAGAAGCTTCCACAAGAGCAGCACTCGGAGGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTGCTGTTGACTGGTAAGGTTCAAGAAGCTATCCCATACTTGGAGGGTGCAGCAGAGACTTTAAAAGAGAGTTTTGGGTCGAAGCATTTTGGAGTTGGATATATATACAATAATCTTGGAGCTGCGTATTTGGAACTTGACAGACCTCAATCAGCTGCGCAAATGTTTGAAGTGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCCTGTCACAATGTTTCAAAGGCATATGGTGCAATGGGAAGTTATGCCCTTGCAATTGAATTCCAGGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCGACTGCCGAAGACGAACTTAGAGAAGCCCGACGAACGCTAGAACAATTGAAGAGGAAAGCTCAAGGTACATCCAAATCCGAGCTCTCAACAAAGGCCTTGCCCCTACCTCATTCACCAACGTCTTCCAAAACTCTGCAGCCTGATCTCCCTGCCCGTCAGAAACATCAATAACAACAAGATCTGATAATATGCTATTTTATGACTTAAGATAAATCGATGGATCATACCACACTCGTTGGTAAGTTTTATCCTAAAAGAACTGATATTTTTAGGGAAAATATTTTCCTTTAAAAAGTTGGTCATCTTGTGCATCGCTGGAATATCCTTGAAAAATATACAAGACTCCCAAGTTTTGCTTTCATGTCTATATATATATCCTGCTGGGAAACATATTCATTGGAAGCTATATGCTTGGGAAAGAGACGGCTGCAGTAGCTCGTAGAGTTATCGAATCTAACGGCTGCGTTTTCTGAGAACTTTGCATCAACTCATCTTGAAAAAGTTCATAGATGTTTATTTAGACTGGTCTTTCAGAATTACATGTATGTAAATGAGTTTGTAATTTTGGTTTTAGTTGCAAAACTCTAATGTAAGAAAGAAAGTTTCTGGGTTGCCTTGAGTTTGGTTTTAGAAAGAAACACATCTCATTTTGTTCTTGTGTTTTTTCTAAGTTTATTACTATTTCAAGTGGCTTTGATTTGGTTGAAAAA

Coding sequence (CDS)

ATGAGAAGAAACGCTTCAATTTCCATTCTTTCTCACTCCAATCGTCTCAATCTCCGCTTACTTTCATCCCTCATTTCCCCAAATTCGATTTCCAATCCACCTTCAAATTCCTCCCCTGTTTCAACTCCCAATCTCAAGCCCTGCACCAAAAATAATGGATTGTTCTTCAAGTTCACTCGATTTCAAGCAAACCCATCTCGAAATTTGGATACCCTTGTGGAGAAGCGGCCTGAAATTTCCTCAAGACAGCGAAAACTCATGGAAAAATCCGAGCTTGAGGAGGTTTTTGAGTCTGCTGACACTGTTGATGAAATGCTCAAGGCGTTTAAAGACATGGAATCCGCTTTTGAAGAGAAGGATCTCGGTTTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAACAAGGTGAGGATCCTGAACAGATTTTGTTGTATGCTAATAGAGCTTTAAAAGCTTTGGATAAAGATGATGATAGAAATCCTTCTTTACCTGTTGCTATGACTTTACAATTGATTGGTTCTGTTAATTATAGTTTGAAGAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCCAATAGGATTCTGGGTCAGTTAGAGAAGAAGGGTTTTAGTGCTGAGGATCTTAGGCCAGTACTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAAACTGCAATGGGGAGAAGAGAAGAAGCGCTTTCTAATCTCAGGAAGTCTTTAGATATTAAGGAGTTGATGTTGGAAAAGGATAGTAGAGAATTAGGGACGGCGAATCGTGATTTGGCCGAAGCTTATGTTGCAATATTGAACTTTAAGGATGCTCTTCCCTTTTGTATGAAGGGATTGGAGATACATAAGAAGCAGTTGGGCAATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTTATTTATAGTGGCTTGGAAGAACATCAAAAGGCATTGGAGCAGAATGAATTGTCTCGGAGGGTTTTGAAGAACTGGGGCCTTAATTCTGATTTGCTTCGTGCTGAAATCGATGCTGCTAATATGCAAATTTCACTCGGTAGGTACGATGAGGCCATTAGTACTCTTAAGGAAGTTGTTCAGCATACAGATAAAGACAGTGAAATTCGGGGAGTTGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTGCTGATGCAAAGAGATGTTTGGCAATTGCTAGTGGAATTTTCGACAAGAGAGAAGGAACTTCTCCTGTGGAAGCTGCTGAGGCGTTTTCGGAGATATCAATGCAGTATGAGACAATGAATGACTTTGAAACTGCAATTTCTTTATTAAAAAGATCTCTAGCTTTGCTTGAGAAGCTTCCACAAGAGCAGCACTCGGAGGGTAGTGTTTCAGCTAGATTAGGGTGGTTGCTGCTGTTGACTGGTAAGGTTCAAGAAGCTATCCCATACTTGGAGGGTGCAGCAGAGACTTTAAAAGAGAGTTTTGGGTCGAAGCATTTTGGAGTTGGATATATATACAATAATCTTGGAGCTGCGTATTTGGAACTTGACAGACCTCAATCAGCTGCGCAAATGTTTGAAGTGGCAAAGGACATTATGGACGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCCTGTCACAATGTTTCAAAGGCATATGGTGCAATGGGAAGTTATGCCCTTGCAATTGAATTCCAGGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCGACTGCCGAAGACGAACTTAGAGAAGCCCGACGAACGCTAGAACAATTGAAGAGGAAAGCTCAAGGTACATCCAAATCCGAGCTCTCAACAAAGGCCTTGCCCCTACCTCATTCACCAACGTCTTCCAAAACTCTGCAGCCTGATCTCCCTGCCCGTCAGAAACATCAATAA

Protein sequence

MRRNASISILSHSNRLNLRLLSSLISPNSISNPPSNSSPVSTPNLKPCTKNNGLFFKFTRFQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEKDLGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSLKRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKSLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAISTLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAEAFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQGTSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ
Homology
BLAST of PI0009344 vs. ExPASy Swiss-Prot
Match: Q9LII8 (Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 3.5e-43
Identity = 141/519 (27.17%), Postives = 249/519 (47.98%), Query Frame = 0

Query: 117 EEKDLGLASLKIGLKLDQQGEDPEQILLYANRALKALDK--DDDRNPSLPVAMTLQLIGS 176
           E  +LG+  LK   +L   GE+  + L  A RA+K  +K  + ++   L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 177 VNYSLKRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEAL 236
           +   L R++D++  L R+  I   +E      ED      A  ++L ++   MG+ E ++
Sbjct: 209 IYAGLGRYNDAVPVLERSIEI-PMIE----DGEDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 237 SNLRKSLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQ--LGN 296
                 L+I+  +L +    +G   R LAEA+V  + F++A   C   L+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 297 NSVEVAHDRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLG 356
            S+E A DR+L+G+I     +++ ALE   L+   + +     D+   +    +  +SL 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 357 RYDEAISTLKEVV----QHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDK 416
           R+DEAI   ++ +    Q   +      +V++ +  +     K  D+K     A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 417 -REGTSPVEAAEAFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGW 476
              GT   E A  F EIS  Y++MN+ + A+ LL+R+L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 477 LLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVA 536
           +  + G   E+    + A    + S   K    G   N +G A ++      AA +FE A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 537 KDIMDVSLGPHHADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREA 596
           K I++   GP+H D++    N++  Y AMG    AIE  E VV   E    TA  E+ + 
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 597 RRTLEQLKRKA-QGTSKSELSTKALPLPHSPTSSKTLQP 626
           ++ L  L ++A +G SK   +   L L ++P  +   +P
Sbjct: 629 KQRLAALLKEAGRGRSKRNRALLTL-LDNNPEIANGQRP 661

BLAST of PI0009344 vs. ExPASy Swiss-Prot
Match: O81629 (Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 4.6e-43
Identity = 153/581 (26.33%), Postives = 272/581 (46.82%), Query Frame = 0

Query: 35  SNSSPVSTPNLKPCTKNNGLFFKFTRFQANPSRNLDTLVEKRPEISSRQRKLMEKSELEE 94
           S  +P  TP L+    +       T+  +NP R   T ++K P  +  + K        +
Sbjct: 9   SVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTPSSTPSRSKPSPNRSTGK 68

Query: 95  VFESADTVDEMLKAFKDMES-AFEEKDLGLASLKIGLKLDQQGEDPEQILLYANRALKAL 154
             + + TV     A  D++  + +  DLG   LK+       GE P + L YA RA K+ 
Sbjct: 69  --KDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSF 128

Query: 155 DK-----------DDDRNPSLPVAMTLQLIGSVNYSLKRFSDSLGYLNRANRILGQLEKK 214
           ++             D  P L +AM+L ++ ++  SL RF +++  L RA ++       
Sbjct: 129 ERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTR-- 188

Query: 215 GFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKSLDIKELMLEKDSRELGTANRDL 274
                D      +  ++L +  + +G+ + +++   + L I+   L      +G   R L
Sbjct: 189 ---GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYL 248

Query: 275 AEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHDRRLLGVIYSGLEEHQKALEQNE 334
           AEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   
Sbjct: 249 AEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLV 308

Query: 335 LSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAISTLKEVV----QHTDKDSEIRGVV 394
           L+   +   G  S++   ++   N+ +SL R+DEA+ + ++ +        +       V
Sbjct: 309 LASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASV 368

Query: 395 FISMGKVLCNQEKFADAKRCLAIASGIFDKR-EGTSPVEAAEAFSEISMQYETMNDFETA 454
           F+ + ++     K  ++K     A  I++K   GT+  E A   +EIS  YE++++ E A
Sbjct: 369 FVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEA 428

Query: 455 ISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKESFGSKH 514
           + LL++S+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ + G K 
Sbjct: 429 LKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKS 488

Query: 515 FGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHADSIEACHNVSKAYGAMG 574
              G + N +G A ++L +   A ++FE A+ I++   GP   D++    N++  Y AMG
Sbjct: 489 AFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMG 548

Query: 575 SYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKA 599
               AIE  EQV+   E    TA  +  + ++ L +L ++A
Sbjct: 549 RIEDAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEA 571

BLAST of PI0009344 vs. ExPASy Swiss-Prot
Match: F4HSX9 (Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 5.2e-39
Identity = 135/518 (26.06%), Postives = 243/518 (46.91%), Query Frame = 0

Query: 113 ESAFEEKDLGLASLKIGLKLDQQGEDPEQILLYANRALKALDKD-DDRNPSLPVAMTLQL 172
           E + E  +L    L     L   G+   + L   +RA K  +   ++  P L   M L +
Sbjct: 138 EPSSENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHV 197

Query: 173 IGSVNYSLKRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRRE 232
             +V+  LK +++++  L R+  I   + ++G   E+      A  ++L +    +G+ E
Sbjct: 198 TAAVHCKLKEYNEAIPVLQRSVEI--PVVEEG---EEHALAKFAGLMQLGDTYAMVGQLE 257

Query: 233 EALSNLRKSLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLG 292
            ++S   + L+I++ +L ++   +G   R LAEA V  L F +A   C   L IH++   
Sbjct: 258 SSISCYTEGLNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGL 317

Query: 293 NNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISL 352
             S+  A DRRL+G+I     +H+ ALE   L+   +   G  S++   +    +  +SL
Sbjct: 318 PGSIAEAADRRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSL 377

Query: 353 GRYDEAI----STLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFD 412
            R+DEAI     +L  +     ++    G V+I +  +     K  +AK     A  I++
Sbjct: 378 SRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYE 437

Query: 413 KRE-GTSPVEAAEAFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLG 472
                 SP E A   ++IS+  E+MN+ E AI+LL+++L +    P ++     + A++G
Sbjct: 438 SHNLEISPEEIASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMG 497

Query: 473 WLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEV 532
            L  + GK  E+    + A   L+ +   +    G   N +G A ++LD  + A ++FE 
Sbjct: 498 VLYYMMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEE 557

Query: 533 AKDIMDVSLGPHHADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELRE 592
           AK I++   GP+H +++    N++ AY A+G    AI+    VV   E    TA     +
Sbjct: 558 AKCILEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTED 617

Query: 593 ARRTLEQLKRKAQGTSKSELSTKALPLPHSPTSSKTLQ 625
            +R L QL ++A   +  +  +    +    TSS  L+
Sbjct: 618 EKRRLAQLLKEAGNVTGRKAKSLKTLIDSDLTSSSALR 650

BLAST of PI0009344 vs. ExPASy Swiss-Prot
Match: A0JM23 (Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 1.8e-15
Identity = 81/353 (22.95%), Postives = 150/353 (42.49%), Query Frame = 0

Query: 227  MGRREEALSNLRKSLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIH 286
            +G   +A++ L++SL+I+E  L+ D   +  +   LA  YV    F +A     + LEI 
Sbjct: 940  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYVQSKKFGNAEQLYKQALEIS 999

Query: 287  KKQLGNNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAAN 346
            +   G+  + VA +   L V+Y    + Q   EQ E  R+  K+  +     R +     
Sbjct: 1000 ENAYGSEHMRVARELDALAVLY----QKQNKFEQAEQLRK--KSLKIRQKSARRKGSMYG 1059

Query: 347  MQISLGRYDEAISTLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIF 406
              +   R       L+E+     KD+         +G +   Q     A+  L  +  + 
Sbjct: 1060 FALLRRR----ALQLEELT--LGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMR 1119

Query: 407  DKREGTSPVEAAEAFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSAR-L 466
            ++  G    + A++ + ++  Y     ++ A  L +R+L +  +     H   + + + L
Sbjct: 1120 ERVLGADHPDCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHL 1179

Query: 467  GWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFE 526
              L    GK+ +A+P  E A E  ++SFG KH  V     NL   Y ++ +   A+ ++E
Sbjct: 1180 AVLYKRKGKLDKAVPLYELAVEIRQKSFGPKHPSVATALVNLAVLYCQMKKQAEASPLYE 1239

Query: 527  VAKDIMDVSLGPHHADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGS 579
             A  I + SLG  H    E   N++      G +  A E  ++ ++  E+  S
Sbjct: 1240 RAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDFEKAAELYKRAMEIKETETS 1280

BLAST of PI0009344 vs. ExPASy Swiss-Prot
Match: Q6AZT7 (Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 4.0e-15
Identity = 80/353 (22.66%), Postives = 149/353 (42.21%), Query Frame = 0

Query: 227  MGRREEALSNLRKSLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIH 286
            +G   +A++ L++SL+I+E  L+ D   +  +   LA  Y+    F +A     + LEI 
Sbjct: 929  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYMQSKKFGNAEQLYKQALEIS 988

Query: 287  KKQLGNNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAAN 346
            +   G+  + VA +   L V+Y    + Q   EQ E  R+  K+  +     R +     
Sbjct: 989  ENAYGSEHLRVARELDALAVLY----QKQNKFEQAEQLRK--KSLKIRQKSARRKGSMYG 1048

Query: 347  MQISLGRYDEAISTLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIF 406
              +   R       L+E+     KD+         +G +   Q     A+  L  +  + 
Sbjct: 1049 FALLRRR----ALQLEELT--LGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMR 1108

Query: 407  DKREGTSPVEAAEAFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSAR-L 466
            ++  G    + A++ + ++  Y     ++ A  L +R+L +  +     H   + + + L
Sbjct: 1109 ERVLGADHPDCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHL 1168

Query: 467  GWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFE 526
              L    GK+ +A+P  E A +  ++SFG KH  V     NL   Y ++ +   A  ++E
Sbjct: 1169 AVLYKRKGKLDKAVPLYELAVDIRQKSFGPKHPSVATALVNLAVLYCQMKKQDDALPLYE 1228

Query: 527  VAKDIMDVSLGPHHADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGS 579
             A  I + SLG  H    E   N++      G Y  A E  ++ ++  E+  S
Sbjct: 1229 RAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDYEKAAELYKRAMEIKETETS 1269

BLAST of PI0009344 vs. ExPASy TrEMBL
Match: A0A1S3BR15 (nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 589/634 (92.90%), Postives = 612/634 (96.53%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNPPSNSSPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASISIL + NRLNLR LSSLISPNSISN P NSSPVSTPNLKPCTKNNGLFFKFTR
Sbjct: 1   MRRNASISILPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTR 60

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEKD 120
           FQAN SRNLDTLVEKRPE+SSRQRKL EKS+LEE FESADTVDEM+KAFK+MESAFEEKD
Sbjct: 61  FQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKD 120

Query: 121 LGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKIGLKLDQ+GEDPEQILLYA+RALKALDKDD+RNPSLPVAMTLQLIGSVNYSLK
Sbjct: 121 LGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180

Query: 181 RFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKS 240
           RFSDSLGYLNRANRILGQLE+KGFSAED+RPVLHAVQLELANVKTAMGRREEALSNLRKS
Sbjct: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 240

Query: 241 LDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHD 300
           L+IKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKK+LGNNSVEVAHD
Sbjct: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300

Query: 301 RRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIST 360
           RRLLGVIYSGLEEHQKALEQNELSR+VLKNWGLNSDLLRAEI+AANMQI+LGRYDEAIST
Sbjct: 301 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 360

Query: 361 LKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAEA 420
           LK+VVQHTDKDSEIRGVVF SMGKVLCNQEKF D KRCL IA GIFDKRE TSP  AAEA
Sbjct: 361 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 420

Query: 421 FSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           +SEISM YETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAIP
Sbjct: 421 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 480

Query: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHA 540
           YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLEL+RPQSAAQMF VAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540

Query: 541 DSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQG 600
           DSIEACHN+SKAYGAMGSYALAIEFQ+QVVDAWESHGSTAEDELREAR+TL+QLKRKAQG
Sbjct: 541 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 600

Query: 601 TSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           TSK ELSTKALPLPHS TSSKTLQPDLPA QKHQ
Sbjct: 601 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQKHQ 634

BLAST of PI0009344 vs. ExPASy TrEMBL
Match: A0A5A7UWT8 (Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00740 PE=4 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 588/634 (92.74%), Postives = 612/634 (96.53%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNPPSNSSPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASIS+L + NRLNLR LSSLISPNSISN P NSSPVSTPNLKPCTKNNGLFFKFTR
Sbjct: 1   MRRNASISVLPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTR 60

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEKD 120
           FQAN SRNLDTLVEKRPE+SSRQRKL EKS+LEE FESADTVDEM+KAFK+MESAFEEKD
Sbjct: 61  FQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKD 120

Query: 121 LGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKIGLKLDQ+GEDPEQILLYA+RALKALDKDD+RNPSLPVAMTLQLIGSVNYSLK
Sbjct: 121 LGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180

Query: 181 RFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKS 240
           RFSDSLGYLNRANRILGQLE+KGFSAED+RPVLHAVQLELANVKTAMGRREEALSNLRKS
Sbjct: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 240

Query: 241 LDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHD 300
           L+IKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKK+LGNNSVEVAHD
Sbjct: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300

Query: 301 RRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIST 360
           RRLLGVIYSGLEEHQKALEQNELSR+VLKNWGLNSDLLRAEI+AANMQI+LGRYDEAIST
Sbjct: 301 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 360

Query: 361 LKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAEA 420
           LK+VVQHTDKDSEIRGVVF SMGKVLCNQEKF D KRCL IA GIFDKRE TSP  AAEA
Sbjct: 361 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 420

Query: 421 FSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           +SEISM YETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAIP
Sbjct: 421 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 480

Query: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHA 540
           YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLEL+RPQSAAQMF VAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540

Query: 541 DSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQG 600
           DSIEACHN+SKAYGAMGSYALAIEFQ+QVVDAWESHGSTAEDELREAR+TL+QLKRKAQG
Sbjct: 541 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 600

Query: 601 TSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           TSK ELSTKALPLPHS TSSKTLQPDLPA QKHQ
Sbjct: 601 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQKHQ 634

BLAST of PI0009344 vs. ExPASy TrEMBL
Match: A0A0A0K8P3 (TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 PE=4 SV=1)

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 579/634 (91.32%), Postives = 610/634 (96.21%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNPPSNSSPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASIS+L + NRLNLR LSSLISPNSISN PSNSSPVSTPNLKPCT NNGLFFKFTR
Sbjct: 12  MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSSPVSTPNLKPCTINNGLFFKFTR 71

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEKD 120
           FQANPSRNLDTLVEKRPEISSRQRKL +KS LEE FESA+TVDEM KAFK+MESAFEEKD
Sbjct: 72  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESAETVDEMFKAFKEMESAFEEKD 131

Query: 121 LGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKI LKLD++GEDPEQ+LLYA+RALKALDKDD+RNPSLPVAMTLQLIGSVNYSLK
Sbjct: 132 LGLASLKIALKLDREGEDPEQVLLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 191

Query: 181 RFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKS 240
           RFSDSLGYLNRANRILGQLE+KG+SAED+RPVLHAV LELANVKTAMGRREEALSNLRK 
Sbjct: 192 RFSDSLGYLNRANRILGQLEEKGYSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 251

Query: 241 LDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHD 300
           L+IKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKK+LGNNSVEVAHD
Sbjct: 252 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 311

Query: 301 RRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIST 360
           RRLLGVIYSGLEE+QKALEQNELSR+VLKNWGLNSDLLRAE DAANMQI+LGRYDEAI+T
Sbjct: 312 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 371

Query: 361 LKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAEA 420
           LK+V QHTDKDSE+RGVVF SMGKVLCNQEKF DAKRCL IASGIFDKREGTSPVEAAEA
Sbjct: 372 LKDVAQHTDKDSELRGVVFFSMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 431

Query: 421 FSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           +SEISMQYETMN+F+TAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP
Sbjct: 432 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 491

Query: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHA 540
           YLEGAAETLKESFGS HFGVGYIYNNLGAAYLEL+RPQSAAQMF VAKDIMDVSLGPHHA
Sbjct: 492 YLEGAAETLKESFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 551

Query: 541 DSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQG 600
           DSIEACHN+SKAYGAMGSYALAIEFQEQVVDAW+SHGS+AEDELREARRTLEQLKRKA+G
Sbjct: 552 DSIEACHNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKARG 611

Query: 601 TSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           TSK E STKALPLP S TSSKTLQPDLP RQ+HQ
Sbjct: 612 TSKFEHSTKALPLPQSSTSSKTLQPDLPTRQQHQ 645

BLAST of PI0009344 vs. ExPASy TrEMBL
Match: A0A6J1GPX7 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456463 PE=4 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.9e-303
Identity = 549/635 (86.46%), Postives = 592/635 (93.23%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNP-PSNSSPVSTPNLKPCTKNNGLFFKFT 60
           MRRNASIS+L HSNR+NLR LSSLISP  IS+P PS SSPVS P+L PCTKN+GLFFK +
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKLS 60

Query: 61  RFQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEK 120
           RFQANPSRNL+TLVEKRP+ISSRQRK  EKSELEE FESA+TVDEM+KAFKDMES FEE+
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSL 180
           DLGLASLKIGLKL+Q+ EDPE+IL+YA RALKALDKDD+  PSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRK 240
           KRFSDSLGYLNRANRILGQLE+ GFSAE +RPVLHAVQLELANVKTAMGRREEAL+NLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 SLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAH 300
            L+IKEL LEKDSRELG ANRDLAEAYVAILNFKDALPFCM+ LEIHKKQLG+NSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELS++VLKNWGLNSDLLRAEIDAANMQI+LGRYDEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAE 420
           TLK+VVQHT+KDSE + VVFISMGKVL NQEKFADAKRCL IA+ IFDK+E  SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           A+SEISMQYE+MN+FETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLEL+R QSAAQMF +AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQ 600
           ADSIEAC N++KAYG++GSYALAIEFQEQVVDAWE HGS AEDELREA+R LEQLKRKA 
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           G SK ELS KALPLPHS TSSKTLQPD+P+ QKHQ
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKHQ 635

BLAST of PI0009344 vs. ExPASy TrEMBL
Match: A0A6J1JWL0 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC111488134 PE=4 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.4e-299
Identity = 545/635 (85.83%), Postives = 587/635 (92.44%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNPP-SNSSPVSTPNLKPCTKNNGLFFKFT 60
           MRRNASIS+L HSNR NLR LSSLISP  IS+P  S SSPVST +L PCTKN+GLFFKF 
Sbjct: 1   MRRNASISLLFHSNRFNLRSLSSLISPKFISDPSFSYSSPVSTTHLNPCTKNHGLFFKFK 60

Query: 61  RFQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEK 120
           RFQANPSRNL+TLVEKRP+ISSRQRK  EKSELEE FESA+TVDEM+KAFKDMES FEE+
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSL 180
           DLGLASLKIGLKL+Q+ EDPE+IL+YA RALKALDKDD+   SLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKLSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRK 240
           KRFSDSLGYLNRANRILGQLE+ GFSAE +RPVLHAVQLELANVKTAMGRREEAL+NLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 SLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAH 300
            L+IKEL LEKDSRELG A RDLAEAYVAILNFKDAL FCM+ LEIHKKQLG+NSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKAKRDLAEAYVAILNFKDALLFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELS++VLKNWGLNSDLLRAEIDAANMQI+LGRYDEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAE 420
           TLK+VVQHT+KDSE + VVFISMGKVL NQEKFADAKRCL IA+ IFDK+E  SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           A+SEISMQYE+MN+FETAISLLKRSLALLEKLPQEQHS GSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSVGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLEL+RPQSAAQMF +AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQ 600
           ADSIEAC N++KAYG++GSYALAIEFQEQVVDAWE HGS AEDELREA+R LEQLKRKA 
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           G SK ELS KALPLPHS TSSKTLQPD+P+ Q+HQ
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQEHQ 635

BLAST of PI0009344 vs. NCBI nr
Match: XP_008451256.1 (PREDICTED: nephrocystin-3 [Cucumis melo])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 589/634 (92.90%), Postives = 612/634 (96.53%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNPPSNSSPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASISIL + NRLNLR LSSLISPNSISN P NSSPVSTPNLKPCTKNNGLFFKFTR
Sbjct: 1   MRRNASISILPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTR 60

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEKD 120
           FQAN SRNLDTLVEKRPE+SSRQRKL EKS+LEE FESADTVDEM+KAFK+MESAFEEKD
Sbjct: 61  FQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKD 120

Query: 121 LGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKIGLKLDQ+GEDPEQILLYA+RALKALDKDD+RNPSLPVAMTLQLIGSVNYSLK
Sbjct: 121 LGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180

Query: 181 RFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKS 240
           RFSDSLGYLNRANRILGQLE+KGFSAED+RPVLHAVQLELANVKTAMGRREEALSNLRKS
Sbjct: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 240

Query: 241 LDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHD 300
           L+IKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKK+LGNNSVEVAHD
Sbjct: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300

Query: 301 RRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIST 360
           RRLLGVIYSGLEEHQKALEQNELSR+VLKNWGLNSDLLRAEI+AANMQI+LGRYDEAIST
Sbjct: 301 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 360

Query: 361 LKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAEA 420
           LK+VVQHTDKDSEIRGVVF SMGKVLCNQEKF D KRCL IA GIFDKRE TSP  AAEA
Sbjct: 361 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 420

Query: 421 FSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           +SEISM YETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAIP
Sbjct: 421 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 480

Query: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHA 540
           YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLEL+RPQSAAQMF VAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540

Query: 541 DSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQG 600
           DSIEACHN+SKAYGAMGSYALAIEFQ+QVVDAWESHGSTAEDELREAR+TL+QLKRKAQG
Sbjct: 541 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 600

Query: 601 TSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           TSK ELSTKALPLPHS TSSKTLQPDLPA QKHQ
Sbjct: 601 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQKHQ 634

BLAST of PI0009344 vs. NCBI nr
Match: KAA0059648.1 (nephrocystin-3 [Cucumis melo var. makuwa] >TYK08180.1 nephrocystin-3 [Cucumis melo var. makuwa])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 588/634 (92.74%), Postives = 612/634 (96.53%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNPPSNSSPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASIS+L + NRLNLR LSSLISPNSISN P NSSPVSTPNLKPCTKNNGLFFKFTR
Sbjct: 1   MRRNASISVLPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTR 60

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEKD 120
           FQAN SRNLDTLVEKRPE+SSRQRKL EKS+LEE FESADTVDEM+KAFK+MESAFEEKD
Sbjct: 61  FQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKD 120

Query: 121 LGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKIGLKLDQ+GEDPEQILLYA+RALKALDKDD+RNPSLPVAMTLQLIGSVNYSLK
Sbjct: 121 LGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180

Query: 181 RFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKS 240
           RFSDSLGYLNRANRILGQLE+KGFSAED+RPVLHAVQLELANVKTAMGRREEALSNLRKS
Sbjct: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 240

Query: 241 LDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHD 300
           L+IKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKK+LGNNSVEVAHD
Sbjct: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300

Query: 301 RRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIST 360
           RRLLGVIYSGLEEHQKALEQNELSR+VLKNWGLNSDLLRAEI+AANMQI+LGRYDEAIST
Sbjct: 301 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 360

Query: 361 LKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAEA 420
           LK+VVQHTDKDSEIRGVVF SMGKVLCNQEKF D KRCL IA GIFDKRE TSP  AAEA
Sbjct: 361 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 420

Query: 421 FSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           +SEISM YETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAIP
Sbjct: 421 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 480

Query: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHA 540
           YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLEL+RPQSAAQMF VAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540

Query: 541 DSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQG 600
           DSIEACHN+SKAYGAMGSYALAIEFQ+QVVDAWESHGSTAEDELREAR+TL+QLKRKAQG
Sbjct: 541 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 600

Query: 601 TSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           TSK ELSTKALPLPHS TSSKTLQPDLPA QKHQ
Sbjct: 601 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQKHQ 634

BLAST of PI0009344 vs. NCBI nr
Match: XP_004148956.2 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucumis sativus] >KGN44742.1 hypothetical protein Csa_016654 [Cucumis sativus])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 579/634 (91.32%), Postives = 610/634 (96.21%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNPPSNSSPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASIS+L + NRLNLR LSSLISPNSISN PSNSSPVSTPNLKPCT NNGLFFKFTR
Sbjct: 12  MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSSPVSTPNLKPCTINNGLFFKFTR 71

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEKD 120
           FQANPSRNLDTLVEKRPEISSRQRKL +KS LEE FESA+TVDEM KAFK+MESAFEEKD
Sbjct: 72  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESAETVDEMFKAFKEMESAFEEKD 131

Query: 121 LGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKI LKLD++GEDPEQ+LLYA+RALKALDKDD+RNPSLPVAMTLQLIGSVNYSLK
Sbjct: 132 LGLASLKIALKLDREGEDPEQVLLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 191

Query: 181 RFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKS 240
           RFSDSLGYLNRANRILGQLE+KG+SAED+RPVLHAV LELANVKTAMGRREEALSNLRK 
Sbjct: 192 RFSDSLGYLNRANRILGQLEEKGYSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 251

Query: 241 LDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHD 300
           L+IKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKK+LGNNSVEVAHD
Sbjct: 252 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 311

Query: 301 RRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIST 360
           RRLLGVIYSGLEE+QKALEQNELSR+VLKNWGLNSDLLRAE DAANMQI+LGRYDEAI+T
Sbjct: 312 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 371

Query: 361 LKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAEA 420
           LK+V QHTDKDSE+RGVVF SMGKVLCNQEKF DAKRCL IASGIFDKREGTSPVEAAEA
Sbjct: 372 LKDVAQHTDKDSELRGVVFFSMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 431

Query: 421 FSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           +SEISMQYETMN+F+TAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP
Sbjct: 432 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 491

Query: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHA 540
           YLEGAAETLKESFGS HFGVGYIYNNLGAAYLEL+RPQSAAQMF VAKDIMDVSLGPHHA
Sbjct: 492 YLEGAAETLKESFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 551

Query: 541 DSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQG 600
           DSIEACHN+SKAYGAMGSYALAIEFQEQVVDAW+SHGS+AEDELREARRTLEQLKRKA+G
Sbjct: 552 DSIEACHNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKARG 611

Query: 601 TSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           TSK E STKALPLP S TSSKTLQPDLP RQ+HQ
Sbjct: 612 TSKFEHSTKALPLPQSSTSSKTLQPDLPTRQQHQ 645

BLAST of PI0009344 vs. NCBI nr
Match: XP_038899312.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 576/635 (90.71%), Postives = 604/635 (95.12%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNP-PSNSSPVSTPNLKPCTKNNGLFFKFT 60
           MRRNASIS+LSHSNRLNLR LSS IS NSISNP PSNSSPVSTPNLKPCTKN+GLFFKFT
Sbjct: 1   MRRNASISLLSHSNRLNLRSLSSFISSNSISNPSPSNSSPVSTPNLKPCTKNHGLFFKFT 60

Query: 61  RFQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEK 120
           RFQANPSRNLDTLVEKRP+ISSRQRK+ EKSELEE FESADTVDEM+KAFKDMESAFEE+
Sbjct: 61  RFQANPSRNLDTLVEKRPQISSRQRKVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120

Query: 121 DLGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSL 180
           DLGLASLKIGLKLDQ+GEDPE ILLYANRALKALDKD + NPSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPENILLYANRALKALDKDVNGNPSLPVAMTLQLMGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRK 240
           KRFSDSLGYLNRANRILGQL +KGFSAED+RPVLHAVQLELANVKTAMGRREEALSNLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLVEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 SLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAH 300
            L+IKEL LEKDSRELG ANRDLAEAYVA+LNFKDALPFCMK LEIHK QLG+NSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGNANRDLAEAYVAVLNFKDALPFCMKALEIHKMQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIS 360
           DRRLL VIYSGLEEHQKALEQNELS++VLKNWGLNSDLLRAEIDAANMQI+LGRY+EAIS
Sbjct: 301 DRRLLAVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYNEAIS 360

Query: 361 TLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAE 420
           TLK+VVQHTDKDSE R VVFISMGKVLCNQEKF D KRCL IASGIFDKRE TSPVEAAE
Sbjct: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDPKRCLEIASGIFDKREATSPVEAAE 420

Query: 421 AFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           A+SEISMQYETMN+FETAISLLK+SLALLEKLP EQHSEGSVSARLGWLLLLTGKVQEAI
Sbjct: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPHEQHSEGSVSARLGWLLLLTGKVQEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLEL+RPQSAAQMF +AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQ 600
           ADSIEAC N+SKAYGAMGSY LAIEFQEQVVDAWESHGSTAEDELREA++ L+QLKRKA 
Sbjct: 541 ADSIEACRNLSKAYGAMGSYPLAIEFQEQVVDAWESHGSTAEDELREAQQMLKQLKRKAH 600

Query: 601 GTSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           GTSK ELSTKALPLPHS TSSKTLQPDLPARQK Q
Sbjct: 601 GTSKIELSTKALPLPHSSTSSKTLQPDLPARQKRQ 635

BLAST of PI0009344 vs. NCBI nr
Match: XP_023549125.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1054.3 bits (2725), Expect = 4.2e-304
Identity = 550/635 (86.61%), Postives = 593/635 (93.39%), Query Frame = 0

Query: 1   MRRNASISILSHSNRLNLRLLSSLISPNSISNP-PSNSSPVSTPNLKPCTKNNGLFFKFT 60
           MRRNASIS+L HSNR+NLR LSSLISP  IS+P PS SSPVS P+L PCTKN+GLFFKF 
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKFN 60

Query: 61  RFQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEK 120
           RFQA+PSRNL+TLVEKRP+ISSRQRK  EKSELEE FESA+TVDEM+KAFKDMES FEE+
Sbjct: 61  RFQASPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSL 180
           DLGLASLKIGLKL+Q+ EDPE+IL+YA RALKALDKDD+  PSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRK 240
           KRFSDSLGYLNRANRILGQLE+ GFSAE +RPVLHAVQLELANVKTAMGRREEAL+NLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 SLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAH 300
            L+IKEL LEKDSRELG ANRDLAEAYVAILNFKDALPFCM+ LEIHKKQLG+NSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIS 360
           DRRLLGVIYSGLEEHQKALEQNELS++VLKNWGLNSDLLRAEIDAANMQI+LGRYDEAIS
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAE 420
           TLK+VVQHT+KDSE + VVFISMGKVL NQEKFADAKRCL IA+ IFDK+E  SPV AAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVAAAE 420

Query: 421 AFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           A+SEISMQYE+MN+FETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHH 540
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLEL+RPQSAAQMF +AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQ 600
           ADSIEAC N++KAYG++GSYALAIEFQEQVVDAWE HGSTAEDELREA+R LEQLKRKA 
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSTAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKSELSTKALPLPHSPTSSKTLQPDLPARQKHQ 635
           G SK ELS KALPLPHS TSSKTLQPD+P+ QKHQ
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKHQ 635

BLAST of PI0009344 vs. TAIR 10
Match: AT2G31240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 745.3 bits (1923), Expect = 3.9e-215
Identity = 391/622 (62.86%), Postives = 499/622 (80.23%), Query Frame = 0

Query: 3   RNASISILSHSNRLNLRLLSSLISPNSISNPPSNSSPVSTPN--LKPCTKNNGLFFKFTR 62
           ++ SIS++   +   +R +   I  N+ S+  S+SS +S        C + N L  +  +
Sbjct: 2   KSLSISLIRRLHFHKIRPIPLSIHLNAASSSSSSSSLISPAREISTSCRRTNDLIKR--Q 61

Query: 63  FQANPSRNLDTLVEKRPEISSRQRKLMEKSELEEVFESADTVDEMLKAFKDMESAFEEKD 122
           F  NP RNL+T VE+ PEISS  +   EK +LEE FESA+T DEM++ FK+ME +FE  +
Sbjct: 62  FWVNPYRNLNTHVEEPPEISSSDK---EKIDLEEAFESANTTDEMVRLFKEMELSFEGNE 121

Query: 123 LGLASLKIGLKLDQQGEDPEQILLYANRALKALDKDDDRNPSLPVAMTLQLIGSVNYSLK 182
           LGL++LK+GL LD++GEDPE++L YA++ALK+ D D ++ P+L VAM  QL+GS NY LK
Sbjct: 122 LGLSALKLGLHLDREGEDPEKVLSYADKALKSFDGDGNK-PNLLVAMASQLMGSANYGLK 181

Query: 183 RFSDSLGYLNRANRILGQLEKKG-FSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRK 242
           RFSDSLGYLNRANRIL +LE  G    ED+RPVLHAVQLELANVK AMGRREEA+ NL+K
Sbjct: 182 RFSDSLGYLNRANRILVKLEADGDCVVEDVRPVLHAVQLELANVKNAMGRREEAIENLKK 241

Query: 243 SLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAH 302
           SL+IKE+  ++DS+E+G ANR LA+AYVA+LNF +ALP+ +K LEIHKK+LGNNS EVA 
Sbjct: 242 SLEIKEMTFDEDSKEMGVANRSLADAYVAVLNFNEALPYALKALEIHKKELGNNSAEVAQ 301

Query: 303 DRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAIS 362
           DRRLLGVIYSGLE+H KALEQN LS+RVLKNWG+  +L+RAEIDAANM+++LG+Y+EAI 
Sbjct: 302 DRRLLGVIYSGLEQHDKALEQNRLSQRVLKNWGMKLELIRAEIDAANMKVALGKYEEAID 361

Query: 363 TLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDKREGTSPVEAAE 422
            LK VVQ TDKDSE+R +VFISM K L NQ+KFA++KRCL  A  I +K+E   PVE AE
Sbjct: 362 ILKSVVQQTDKDSEMRAMVFISMSKALVNQQKFAESKRCLEFACEILEKKETALPVEVAE 421

Query: 423 AFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 482
           A+SE++MQYE+MN+FETAISLL+++L +LEKLPQEQHSEGSVSAR+GWLLL +G+V +A+
Sbjct: 422 AYSEVAMQYESMNEFETAISLLQKTLGILEKLPQEQHSEGSVSARIGWLLLFSGRVSQAV 481

Query: 483 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHH 542
           PYLE AAE LKESFG+KHFGVGY+YNNLGAAYLEL RPQSAAQMF VAKDIMDVSLGP+H
Sbjct: 482 PYLESAAERLKESFGAKHFGVGYVYNNLGAAYLELGRPQSAAQMFAVAKDIMDVSLGPNH 541

Query: 543 ADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKAQ 602
            DSI+AC N+SKAY  MG+Y+LA+EFQ++V++AW++HG +A+DE++EA+R LE L+ KA+
Sbjct: 542 VDSIDACQNLSKAYAGMGNYSLAVEFQQRVINAWDNHGDSAKDEMKEAKRLLEDLRLKAR 601

Query: 603 -GTSKSELSTKALPLPHSPTSS 621
            G S ++L  KALPLP    SS
Sbjct: 602 GGVSTNKLLNKALPLPKPSHSS 617

BLAST of PI0009344 vs. TAIR 10
Match: AT3G27960.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 177.9 bits (450), Expect = 2.5e-44
Identity = 141/519 (27.17%), Postives = 249/519 (47.98%), Query Frame = 0

Query: 117 EEKDLGLASLKIGLKLDQQGEDPEQILLYANRALKALDK--DDDRNPSLPVAMTLQLIGS 176
           E  +LG+  LK   +L   GE+  + L  A RA+K  +K  + ++   L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 177 VNYSLKRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRREEAL 236
           +   L R++D++  L R+  I   +E      ED      A  ++L ++   MG+ E ++
Sbjct: 209 IYAGLGRYNDAVPVLERSIEI-PMIE----DGEDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 237 SNLRKSLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQ--LGN 296
                 L+I+  +L +    +G   R LAEA+V  + F++A   C   L+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 297 NSVEVAHDRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISLG 356
            S+E A DR+L+G+I     +++ ALE   L+   + +     D+   +    +  +SL 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 357 RYDEAISTLKEVV----QHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFDK 416
           R+DEAI   ++ +    Q   +      +V++ +  +     K  D+K     A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 417 -REGTSPVEAAEAFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGW 476
              GT   E A  F EIS  Y++MN+ + A+ LL+R+L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 477 LLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEVA 536
           +  + G   E+    + A    + S   K    G   N +G A ++      AA +FE A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 537 KDIMDVSLGPHHADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELREA 596
           K I++   GP+H D++    N++  Y AMG    AIE  E VV   E    TA  E+ + 
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 597 RRTLEQLKRKA-QGTSKSELSTKALPLPHSPTSSKTLQP 626
           ++ L  L ++A +G SK   +   L L ++P  +   +P
Sbjct: 629 KQRLAALLKEAGRGRSKRNRALLTL-LDNNPEIANGQRP 661

BLAST of PI0009344 vs. TAIR 10
Match: AT4G10840.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 3.2e-44
Identity = 153/581 (26.33%), Postives = 272/581 (46.82%), Query Frame = 0

Query: 35  SNSSPVSTPNLKPCTKNNGLFFKFTRFQANPSRNLDTLVEKRPEISSRQRKLMEKSELEE 94
           S  +P  TP L+    +       T+  +NP R   T ++K P  +  + K        +
Sbjct: 9   SVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTPSSTPSRSKPSPNRSTGK 68

Query: 95  VFESADTVDEMLKAFKDMES-AFEEKDLGLASLKIGLKLDQQGEDPEQILLYANRALKAL 154
             + + TV     A  D++  + +  DLG   LK+       GE P + L YA RA K+ 
Sbjct: 69  --KDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSF 128

Query: 155 DK-----------DDDRNPSLPVAMTLQLIGSVNYSLKRFSDSLGYLNRANRILGQLEKK 214
           ++             D  P L +AM+L ++ ++  SL RF +++  L RA ++       
Sbjct: 129 ERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTR-- 188

Query: 215 GFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKSLDIKELMLEKDSRELGTANRDL 274
                D      +  ++L +  + +G+ + +++   + L I+   L      +G   R L
Sbjct: 189 ---GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYL 248

Query: 275 AEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHDRRLLGVIYSGLEEHQKALEQNE 334
           AEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   
Sbjct: 249 AEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLV 308

Query: 335 LSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAISTLKEVV----QHTDKDSEIRGVV 394
           L+   +   G  S++   ++   N+ +SL R+DEA+ + ++ +        +       V
Sbjct: 309 LASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASV 368

Query: 395 FISMGKVLCNQEKFADAKRCLAIASGIFDKR-EGTSPVEAAEAFSEISMQYETMNDFETA 454
           F+ + ++     K  ++K     A  I++K   GT+  E A   +EIS  YE++++ E A
Sbjct: 369 FVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEA 428

Query: 455 ISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKESFGSKH 514
           + LL++S+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ + G K 
Sbjct: 429 LKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKS 488

Query: 515 FGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHADSIEACHNVSKAYGAMG 574
              G + N +G A ++L +   A ++FE A+ I++   GP   D++    N++  Y AMG
Sbjct: 489 AFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMG 548

Query: 575 SYALAIEFQEQVVDAWESHGSTAEDELREARRTLEQLKRKA 599
               AIE  EQV+   E    TA  +  + ++ L +L ++A
Sbjct: 549 RIEDAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEA 571

BLAST of PI0009344 vs. TAIR 10
Match: AT4G10840.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 1.7e-40
Identity = 141/540 (26.11%), Postives = 252/540 (46.67%), Query Frame = 0

Query: 35  SNSSPVSTPNLKPCTKNNGLFFKFTRFQANPSRNLDTLVEKRPEISSRQRKLMEKSELEE 94
           S  +P  TP L+    +       T+  +NP R   T ++K P  +  + K        +
Sbjct: 9   SVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTPSSTPSRSKPSPNRSTGK 68

Query: 95  VFESADTVDEMLKAFKDMES-AFEEKDLGLASLKIGLKLDQQGEDPEQILLYANRALKAL 154
             + + TV     A  D++  + +  DLG   LK+       GE P + L YA RA K+ 
Sbjct: 69  --KDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSF 128

Query: 155 DK-----------DDDRNPSLPVAMTLQLIGSVNYSLKRFSDSLGYLNRANRILGQLEKK 214
           ++             D  P L +AM+L ++ ++  SL RF +++  L RA ++       
Sbjct: 129 ERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTR-- 188

Query: 215 GFSAEDLRPVLHAVQLELANVKTAMGRREEALSNLRKSLDIKELMLEKDSRELGTANRDL 274
                D      +  ++L +  + +G+ + +++   + L I+   L      +G   R L
Sbjct: 189 ---GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYL 248

Query: 275 AEAYVAILNFKDALPFCMKGLEIHKKQLGNNSVEVAHDRRLLGVIYSGLEEHQKALEQNE 334
           AEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++ ALE   
Sbjct: 249 AEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLV 308

Query: 335 LSRRVLKNWGLNSDLLRAEIDAANMQISLGRYDEAISTLKEVV----QHTDKDSEIRGVV 394
           L+   +   G  S++   ++   N+ +SL R+DEA+ + ++ +        +       V
Sbjct: 309 LASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASV 368

Query: 395 FISMGKVLCNQEKFADAKRCLAIASGIFDKR-EGTSPVEAAEAFSEISMQYETMNDFETA 454
           F+ + ++     K  ++K     A  I++K   GT+  E A   +EIS  YE++++ E A
Sbjct: 369 FVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEA 428

Query: 455 ISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKESFGSKH 514
           + LL++S+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ + G K 
Sbjct: 429 LKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAA-GEKS 488

Query: 515 FGVGYIYNNLGAAYLELDRPQSAAQMFEVAKDIMDVSLGPHHADSIEACHNVSKAYGAMG 558
              G + N +G A ++L +   A ++FE A+ I++   GP   D++    N++  Y AMG
Sbjct: 489 AFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMG 530

BLAST of PI0009344 vs. TAIR 10
Match: AT1G27500.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 164.1 bits (414), Expect = 3.7e-40
Identity = 135/518 (26.06%), Postives = 243/518 (46.91%), Query Frame = 0

Query: 113 ESAFEEKDLGLASLKIGLKLDQQGEDPEQILLYANRALKALDKD-DDRNPSLPVAMTLQL 172
           E + E  +L    L     L   G+   + L   +RA K  +   ++  P L   M L +
Sbjct: 138 EPSSENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHV 197

Query: 173 IGSVNYSLKRFSDSLGYLNRANRILGQLEKKGFSAEDLRPVLHAVQLELANVKTAMGRRE 232
             +V+  LK +++++  L R+  I   + ++G   E+      A  ++L +    +G+ E
Sbjct: 198 TAAVHCKLKEYNEAIPVLQRSVEI--PVVEEG---EEHALAKFAGLMQLGDTYAMVGQLE 257

Query: 233 EALSNLRKSLDIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKQLG 292
            ++S   + L+I++ +L ++   +G   R LAEA V  L F +A   C   L IH++   
Sbjct: 258 SSISCYTEGLNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGL 317

Query: 293 NNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSRRVLKNWGLNSDLLRAEIDAANMQISL 352
             S+  A DRRL+G+I     +H+ ALE   L+   +   G  S++   +    +  +SL
Sbjct: 318 PGSIAEAADRRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSL 377

Query: 353 GRYDEAI----STLKEVVQHTDKDSEIRGVVFISMGKVLCNQEKFADAKRCLAIASGIFD 412
            R+DEAI     +L  +     ++    G V+I +  +     K  +AK     A  I++
Sbjct: 378 SRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYE 437

Query: 413 KRE-GTSPVEAAEAFSEISMQYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLG 472
                 SP E A   ++IS+  E+MN+ E AI+LL+++L +    P ++     + A++G
Sbjct: 438 SHNLEISPEEIASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMG 497

Query: 473 WLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELDRPQSAAQMFEV 532
            L  + GK  E+    + A   L+ +   +    G   N +G A ++LD  + A ++FE 
Sbjct: 498 VLYYMMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEE 557

Query: 533 AKDIMDVSLGPHHADSIEACHNVSKAYGAMGSYALAIEFQEQVVDAWESHGSTAEDELRE 592
           AK I++   GP+H +++    N++ AY A+G    AI+    VV   E    TA     +
Sbjct: 558 AKCILEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTED 617

Query: 593 ARRTLEQLKRKAQGTSKSELSTKALPLPHSPTSSKTLQ 625
            +R L QL ++A   +  +  +    +    TSS  L+
Sbjct: 618 EKRRLAQLLKEAGNVTGRKAKSLKTLIDSDLTSSSALR 650

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LII83.5e-4327.17Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 P... [more]
O816294.6e-4326.33Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 P... [more]
F4HSX95.2e-3926.06Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 P... [more]
A0JM231.8e-1522.95Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2[more]
Q6AZT74.0e-1522.66Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BR150.0e+0092.90nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1[more]
A0A5A7UWT80.0e+0092.74Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00740... [more]
A0A0A0K8P30.0e+0091.32TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 ... [more]
A0A6J1GPX72.9e-30386.46protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A6J1JWL03.4e-29985.83protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC11148813... [more]
Match NameE-valueIdentityDescription
XP_008451256.10.0e+0092.90PREDICTED: nephrocystin-3 [Cucumis melo][more]
KAA0059648.10.0e+0092.74nephrocystin-3 [Cucumis melo var. makuwa] >TYK08180.1 nephrocystin-3 [Cucumis me... [more]
XP_004148956.20.0e+0091.32protein KINESIN LIGHT CHAIN-RELATED 1 [Cucumis sativus] >KGN44742.1 hypothetical... [more]
XP_038899312.10.0e+0090.71protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida][more]
XP_023549125.14.2e-30486.61protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G31240.13.9e-21562.86Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G27960.12.5e-4427.17Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.13.2e-4426.33Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.21.7e-4026.11Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G27500.13.7e-4026.06Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 577..604
NoneNo IPR availablePFAMPF13424TPR_12coord: 216..287
e-value: 1.4E-7
score: 31.7
coord: 466..530
e-value: 1.6E-9
score: 37.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 594..634
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 600..627
NoneNo IPR availablePANTHERPTHR47459:SF1KINESIN LIGHT CHAIN-RELATEDcoord: 3..623
NoneNo IPR availablePANTHERPTHR47459KINESIN LIGHT CHAIN-RELATEDcoord: 3..623
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 256..289
e-value: 75.0
score: 7.3
coord: 338..371
e-value: 53.0
score: 8.6
coord: 459..492
e-value: 240.0
score: 2.9
coord: 214..247
e-value: 25.0
score: 11.4
coord: 543..576
e-value: 91.0
score: 6.5
coord: 376..409
e-value: 150.0
score: 4.6
coord: 166..199
e-value: 220.0
score: 3.2
coord: 501..534
e-value: 64.0
score: 7.9
coord: 418..451
e-value: 14.0
score: 13.8
IPR019734Tetratricopeptide repeatPFAMPF13181TPR_8coord: 544..571
e-value: 0.029
score: 14.6
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 418..451
score: 8.2899
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 415..611
e-value: 1.2E-27
score: 98.7
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 303..405
e-value: 1.7E-5
score: 26.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 60..289
e-value: 2.9E-15
score: 57.8
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 216..404
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 416..564
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 147..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009344.1PI0009344.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding