PI0009324 (gene) Melon (PI 482460) v1

BLAST of PI0009324 vs. ExPASy Swiss-Prot
Match: Q149N8 (E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2)

HSP 1 Score: 528.5 bits (1360), Expect = 3.0e-148
Identity = 389/1254 (31.02%), Postives = 579/1254 (46.17%), Query Frame = 0

Query: 499  RVECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNSREKRNNR 558
            R EC+CG + +  R     VQC  C  WQHA CV Y  K   +KP               
Sbjct: 658  RFECICGELDQIDRKPR--VQCLKCHLWQHAKCVNYDEKNLKIKPF-------------- 717

Query: 559  STLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKL 618
                         C  CL  M+    P++T ATLI+ P+ I  QW  EI RH R  S+++
Sbjct: 718  ------------YCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSLRV 777

Query: 619  LVYEGVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEG--DRRFMRFQKR 678
            LVY+GV+    L     Q + L   DIV+ +YDVL+ +L++    H    D R +R QKR
Sbjct: 778  LVYQGVKKDGFL-----QPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKR 837

Query: 679  YPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYG 738
            Y  +P+PL  + WWRICLDE QMVE     A EMA RL   + WCI+GTP+QR LEDL+G
Sbjct: 838  YMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDLFG 897

Query: 739  LLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQ 798
            L+ F+   P+ V  WWV ++  PY +++P   +  + F  +I+WRS K  V D++Q+PPQ
Sbjct: 898  LVVFLGIEPYCVKHWWVRLLYRPYCKKNP---QHLYSFIAKILWRSAKKDVIDQIQIPPQ 957

Query: 799  EEQVTWLKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITH 858
             E++ WL FSP+E HFY RQHE C    ++V+  L      RK+ D        D     
Sbjct: 958  TEEIHWLHFSPVERHFYHRQHEVC---CQDVVVKL------RKISDWALKLSSLD----R 1017

Query: 859  ADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSV 918
                 +L  LL+LRQACCHPQ        LQ+S MTMEE+L  L  K   E EEA R+ V
Sbjct: 1018 RTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLV 1077

Query: 919  VALNALAGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVN 978
             ALN LAGI II+ +++ A  LY+E L  +EE+    + D L  +H  HNL E+L     
Sbjct: 1078 CALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELL----- 1137

Query: 979  QSQSPLKDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHP 1038
                                             I +   +  TL         G++ +  
Sbjct: 1138 ---------------------------------IARHPGIPPTL-------RDGRLEEEA 1197

Query: 1039 LQLTGKDTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSY 1098
             QL                                  R+ Y++  +++++ AQQ      
Sbjct: 1198 KQL----------------------------------REHYMSKCNTEVAEAQQAL-YPV 1257

Query: 1099 MQVRSELKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNL--NNSKSRVGSRF 1158
             Q   EL+ + + N  WWL  +H A +      EL++++   ++ N      K  +  +F
Sbjct: 1258 QQTIHELQRKIHSNSPWWLNVIHRAIE-FTIDEELVQRVRNEITSNYKQQTGKLSMSEKF 1317

Query: 1159 RSISALKYHVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPP 1218
            R    L++ + + ++ L   +K+V + +  ++     P    IE    C    A      
Sbjct: 1318 RDCRGLQFLLTTQMEELNKCQKLVREAVKNLEGP---PSRNVIESATVCHLRPARLPLNC 1377

Query: 1219 CVLCELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQK-----KKSALNRFYWSLLQQN 1278
            CV C+ DELF EYE++LF  N V+ G     EE +E ++     +     R  W++    
Sbjct: 1378 CVFCKADELFTEYESKLFS-NTVK-GQTAIFEEMIEDEEGLVDDRAPTTTRGLWAI---- 1437

Query: 1279 KNSSSSKVGHEEPNKRDVGEKVMVSKHPSELEVVLGVIKNFCKT-QLGKESILAANKQLH 1338
                                        SE E  +  I +F K+ +   E +   +  + 
Sbjct: 1438 ----------------------------SETERSMKAILSFAKSHRFDVEFVDEGSTSMD 1497

Query: 1339 LLEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLRD----NDDDSSAYAISEHELP 1398
            L E  +KEY       +A    ++A DE+ MAT RL +RD      +    + I  HE+ 
Sbjct: 1498 LFEAWKKEYKLLHEYWMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEVE 1557

Query: 1399 AASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQ 1458
               ++  +DK ++ + L +  G+L YL  L +S                           
Sbjct: 1558 QNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKS--------------------------- 1617

Query: 1459 KNENTLKADEESCPVCQEKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPT 1518
            +++ +   + E CP+C  +L  Q  V  CGH  C +C+  + E+   GS   +  + C  
Sbjct: 1618 QDKTSGGVNPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSS--IKCAI 1677

Query: 1519 CRQHTDFGNIAYADDSKNETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYT 1578
            CRQ T    I+Y   S+                E  I V+GS+ TK+EAVVR ++ I+  
Sbjct: 1678 CRQTTSHKEISYVFTSE------------KANQEEDIPVKGSHSTKVEAVVRTLMKIQLR 1678

Query: 1579 DSEAKVLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKSQTAISEF-RGQKINAKENKKK 1638
            D  AK LVFS+W DVLD++  A   NN+ F ++   +  Q  +S F R  +IN       
Sbjct: 1738 DPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKRDPQIN------- 1678

Query: 1639 RLSTNLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQEN 1698
                         +LLL +  G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ  
Sbjct: 1798 -------------ILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTK 1678

Query: 1699 KTFVHRFIVKDTVEESIYKLNRSRESSSFITGNAKNQDQPCLTLKEVESLFASK 1738
             T VHRF++K T+EE +  + ++ E  S    +AK+ +   LT+ ++  LF  +
Sbjct: 1858 PTIVHRFLIKATIEERMQAMLKTAE-RSHTNSSAKHSEASVLTVADLADLFTKE 1678

BLAST of PI0009324 vs. ExPASy Swiss-Prot
Match: Q7TPQ3 (E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 3.6e-146
Identity = 385/1254 (30.70%), Postives = 581/1254 (46.33%), Query Frame = 0

Query: 499  RVECLCGAVSE-NYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNSREKRNN 558
            R EC+CG   +  ++ +   VQC  C  WQHA CV Y  K   VKP              
Sbjct: 649  RFECICGEFDQIGHKPR---VQCLKCHLWQHAKCVNYEEKNLKVKPF------------- 708

Query: 559  RSTLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIK 618
                          C  CL  M+    P++T ATLI+ P+ I  QW  EI RH R  S++
Sbjct: 709  -------------YCPHCLVAME----PVSTRATLIISPSSICHQWVDEINRHVRSSSLR 768

Query: 619  LLVYEGVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEG--DRRFMRFQK 678
            +LVY+GV+    L     Q + L   DIV+ +YDVL+ +L++ +  H    D R +R QK
Sbjct: 769  VLVYQGVKKHGFL-----QPHFLAEQDIVIITYDVLRSELNYVNIPHSNSEDGRRLRNQK 828

Query: 679  RYPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLY 738
            RY  +P+PL  + WWRICLDE QMVE     A EMA RL   + WCI+GTP+QR LEDL+
Sbjct: 829  RYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLEDLF 888

Query: 739  GLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPP 798
            GL+ F+   P+ V  WW+ ++  PY +++P   +  + F  +IMWRS K  V D++Q+PP
Sbjct: 889  GLVVFLGIEPYCVKHWWIRLLYHPYCKKNP---QHLYSFIAKIMWRSAKKDVIDQIQIPP 948

Query: 799  QEEQVTWLKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLIT 858
            Q E++ WL FSP+E HFY RQHE C   A   ++ + D  +K      +SS       + 
Sbjct: 949  QTEEMHWLHFSPVERHFYHRQHEVCCQDAIVKLRKISDWALK------LSS-------LD 1008

Query: 859  HADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRS 918
                  +L  LL+LRQACCHPQ        LQ+S MTMEE+L  L  K   E EEA R+ 
Sbjct: 1009 RRTVSSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQL 1068

Query: 919  VVALNALAGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAV 978
            V ALN LAGI II+ +++ A  LY+E L  +EE+    + D L  +H  HNL E+L    
Sbjct: 1069 VCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELL---- 1128

Query: 979  NQSQSPLKDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDH 1038
                   K    P T  ++  R+++  K                                
Sbjct: 1129 -----GAKHPGIPPT--LRDGRLEEEAKQ------------------------------- 1188

Query: 1039 PLQLTGKDTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKS 1098
                                                 +R+ Y++  +++++ AQQ     
Sbjct: 1189 -------------------------------------LREHYMSKCNTEVAEAQQALQPV 1248

Query: 1099 YMQVRSELKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNL--NNSKSRVGSR 1158
               +R EL+ + + N  WWL  +H A        EL++++   +S N      K  +  +
Sbjct: 1249 QQSIR-ELQRKIHSNSPWWLNVIHRA-MEFSVDEELVQRVRNEISSNYKQQTDKLSMSEK 1308

Query: 1159 FRSISALKYHVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGP 1218
            FR    L++ + + ++ L   +K+V + + ++++    P  E IE    C    A     
Sbjct: 1309 FRDCRGLQFLLTTQMEELHKFQKLVREAVKKLEKP---PSREVIESATVCHLRPARLPLN 1368

Query: 1219 PCVLCELDELFQEYEARLFRLNKVQGGMVTSVEEAVEAQK-----KKSALNRFYWSLLQQ 1278
             CV C+ DELF EYE++LF  N V+ G     EE +E ++     +     R  W++   
Sbjct: 1369 CCVFCKADELFTEYESKLF-FNTVK-GQTAIFEEMIEDEEGLVDDRVPTTTRGLWAV--- 1428

Query: 1279 NKNSSSSKVGHEEPNKRDVGEKVMVSKHPSELEVVLGVIKNFCKT-QLGKESILAANKQL 1338
                                         SE E  +  I +F ++ +   E +   +  +
Sbjct: 1429 -----------------------------SETERSMKAILSFARSHRFDVEYVDEGSVSM 1488

Query: 1339 HLLEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDSS----AYAISEHEL 1398
             L E  +KEY       +     ++A DE+ MAT RL +R   +        + I  HE+
Sbjct: 1489 DLFEAWKKEYKLLHEYWMTLRNRVSAVDELAMATERLRVRHPKEPKPNPPVHHIIEPHEV 1548

Query: 1399 PAASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAME 1458
                ++  +DK ++ + L +  G+L YL  L +S+     D +S   +  EP        
Sbjct: 1549 EQNRIKLVNDKAVATSQLQKKLGQLLYLTNLEKSQ-----DKTSG-GINPEP-------- 1608

Query: 1459 QKNENTLKADEESCPVCQEKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCP 1518
                         CP+C  +L  Q  V  CGH  C +C   + E+   GS   +  + C 
Sbjct: 1609 -------------CPICARQLGKQWAVLTCGHCFCNECTSIIIEQYSVGSHRSS--IKCA 1668

Query: 1519 TCRQHTDFGNIAYADDSKNETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKY 1578
             CRQ T    ++Y   S+                E  I V+GS+ TK+EAVVR ++ I+ 
Sbjct: 1669 ICRQTTSHKEVSYVFTSE------------KANQEDDIPVKGSHSTKVEAVVRTLMKIQL 1669

Query: 1579 TDSEAKVLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKK 1638
             D  AK LVFS+W DVLD++  A   NN+ F ++   +  Q  +S F+            
Sbjct: 1729 RDPGAKALVFSTWQDVLDIISKALTDNNMEFTQISRIKTFQENLSAFKYD---------- 1669

Query: 1639 RLSTNLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQEN 1698
                  P +    +LLL +  G+NGL ++EA HV+LVEP+LNPA E QAI RVHRIGQ  
Sbjct: 1789 ------PHI---NILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTK 1669

Query: 1699 KTFVHRFIVKDTVEESIYKLNRSRESSSFITGNAKNQDQPCLTLKEVESLFASK 1738
             T VHRF++K T+EE +  + ++ E  S  + + K+ +   LT+  +  LF  +
Sbjct: 1849 PTIVHRFLIKATIEERMQAMLKTAE-RSHTSSSGKHSEASVLTVAGLADLFTKE 1669

BLAST of PI0009324 vs. ExPASy Swiss-Prot
Match: Q9UTL9 (Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC144.05 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 5.2e-60
Identity = 319/1400 (22.79%), Postives = 558/1400 (39.86%), Query Frame = 0

Query: 369  PNLLPKLRPYQRRAAYWMVQR--EKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNP 428
            P L  +L P+Q R   WM +R  EK  + N         + PL    K L     V+ N 
Sbjct: 212  PRLSSELLPFQMRVLEWMKRREEEKFLTSND--------LPPLWYHCKSLFDDRMVYVNH 271

Query: 429  FSGNLTLNAEQIL----PHVYGGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQ 488
              G +T + E+        + GGILADEMG+GKT+E+L  +L HQ+ +            
Sbjct: 272  VYGYMTFSKEKTYLLASGDIRGGILADEMGMGKTLEVLGLVLHHQLPI------------ 331

Query: 489  QLVKDQRTEFKRLKRERVECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK 548
                                                   + D       VG + K     
Sbjct: 332  --------------------------------------SLTDTCTFDQVVGKNVK----- 391

Query: 549  PIDTEDGNSREKRNNRSTLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQ 608
                                                         + ATLI+ P+ IL Q
Sbjct: 392  --------------------------------------------YSKATLIITPSTILDQ 451

Query: 609  WQAEILRHTRPGSIKLLVYEGVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSD 668
            W +EI  H    S+K+  Y+G+R ++  G  S +I   ++ DIV+TSY  L+ +L +   
Sbjct: 452  WLSEIDLHV--PSLKVFHYQGIRKSN--GLKSAKI--FLDCDIVVTSYSDLRFELLYT-- 511

Query: 669  RHEGDRRFMRFQKRYPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCI 728
              E   R +R +KR+    +PL  + WWRIC+DE QMVE++ +   +M  R+   + W +
Sbjct: 512  --ESHSRTLRHEKRHVSPKSPLIDVCWWRICVDEAQMVETSQSNVAQMIYRIPRVNCWTV 571

Query: 729  TGTPIQRKLEDLYGLLRFVKASPFNVHR--WWVEVIRDPYERRDPGAMEFTHKFFKQIMW 788
            +GTP++ +++DL+GLL  ++ SP  +++   W+++I     R      EF    F  ++ 
Sbjct: 572  SGTPVRSEVDDLFGLLFLLRYSPMYLYKKQAWMQIIEKKRVR------EFC-DLFGSLVC 631

Query: 789  RSLKIHVTDELQLPPQEEQVTWLKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKV 848
            R  K  V +EL+LPPQ       + S +EE  YQ      +S A + +   KD    R +
Sbjct: 632  RHSKQDVEEELKLPPQHRICMTTRLSVVEETNYQ----DLLSEAAKSLHFFKD----RNL 691

Query: 849  PDCVSSDVPSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM-TMEEILMV 908
              C              D   +   L++LRQACCHPQVG     +    PM ++ ++L+ 
Sbjct: 692  DLC--------------DEESMRRWLVRLRQACCHPQVGFGNKSAFGGGPMKSINDVLVF 751

Query: 909  LVSKTRIEGEEALRRSVVALNALAG-IAIIEKKFSEAFSLYKEA---LELAEENNED--- 968
            ++ +T      +L R + +   + G I    K +++A +++ E    +ELA +  E+   
Sbjct: 752  MLEQTN-STFSSLNRKLYSDKIIVGQIYDHIKDYNKALAIWSEVRIPVELAVKELENVIY 811

Query: 969  ---------------FRLDPLLSIHIHHNLAEILPLAVNQSQSPLKDQFYPRTCEVKASR 1028
                           F LD  + + + + L   +   +  +   LK              
Sbjct: 812  NSKYEDHGKNLPINYFGLDHFIHLRVWYVLLHKIYFFIASAYFSLK-------------- 871

Query: 1029 MDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGKDTNAKKEENYEPHRS 1088
               +EK+++  +++             ++D   K++   +  T K               
Sbjct: 872  ---NEKFENEFYLL-------------AQDLRRKIMSDVIIKTSK--------------- 931

Query: 1089 SSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSELKDRENLNDVWWLEA 1148
              + +E S         +K++     K+   Q+ + K  +     ++D   L    + E 
Sbjct: 932  --HLEEFS---------EKFIPKKLVKIPRLQKSYAKGLITGHGIIEDYNRL----YKEL 991

Query: 1149 VHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKYHVQSGLDLLEASRKV 1208
                E    F   LI  ++  +   L+      G  +      +  +   +D+    R++
Sbjct: 992  NDQKEVLIKFRDRLIHLMKLPL---LDQESDPTGDEYEESLNAQSEISYCIDVY---RQM 1051

Query: 1209 VLDRLLEIDQTMEN--PKEEDIERVRYCRNCQADSNGPPCVLCELDE----LFQEYEARL 1268
            + DR+  +  T+      E ++E+ +   + +           E D+     F+E E   
Sbjct: 1052 LSDRVAAVSGTINTFVSHETELEKYKLIESIKKSEKSLDKQAEERDKKYLLYFEEREEAR 1111

Query: 1269 FRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEPNKRDVGEKV 1328
             + ++  G ++  V   ++A  + +       S  + +KN       +E  +     +  
Sbjct: 1112 PKADQ-YGSLINIVSRLLDASNRST-------SSFETSKNMEE----YERIDAMAKEQSR 1171

Query: 1329 MVSKHPSELEVVL----GVIKNFCKTQLGKESILAAN-KQLHLLEYMRKEYGHARSL--A 1388
            +  K   EL ++       I+ + + Q   +S++      + L  Y++ +    + L   
Sbjct: 1172 ICQKLEKELSIIQLTYNSRIEYYKQLQEISDSLMPPPVSNISLNNYVKDDEKKQKFLNSV 1231

Query: 1389 IAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPA---ASVQYSSDKFMSLAML 1448
            I +A V+    EI           N  +     ISE  +P       +   +K  +   +
Sbjct: 1232 IIKASVI-LEKEISEKQDEASQTTNVAELVNQKISEMNIPGHIHLLRELEEEKSNTQRKI 1291

Query: 1449 SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKAD-EESCPVC 1508
            +  + + RYL  L +                                 LKA+  + C +C
Sbjct: 1292 AHFESRRRYLTNLYE------------------------------HIVLKAESHQICIIC 1324

Query: 1509 QEKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDS 1568
            ++ +  Q  +  CGH+ C  CL A  +   H S        CP C+   +  N  Y  +S
Sbjct: 1352 RD-IIKQGFITTCGHLYCSFCLEAWLK---HSSS-------CPMCKTKLNKNNAYYIGES 1324

Query: 1569 K------------NETLDPSTLLETSREHEMS-ITVQGSYGTKIEAVVRRILWIKYTDSE 1628
            +            N+  +   +L+     ++S + ++ S+G+KI+ + + +L++K+ +  
Sbjct: 1412 RDIYSRQEFVTGFNKRDERLEILDDEAYRQISNMELKESFGSKIDTISKHLLYLKHNELY 1324

Query: 1629 AKVLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLST 1688
             KV+VFS W DVLDVL  +F AN I FIR  G  K+ T +  F+                
Sbjct: 1472 PKVVVFSQWLDVLDVLHKSFEANGIVFIRFDGKSKN-TCLKRFK---------------- 1324

Query: 1689 NLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFV 1708
               E RS QVL L  +  ++GL L  A HV + EPLLN   E QAISRVHRIGQ   TFV
Sbjct: 1532 ---EERSLQVLTLHARSQSSGLTLTNATHVFMCEPLLNSGIEMQAISRVHRIGQTRPTFV 1324

BLAST of PI0009324 vs. ExPASy Swiss-Prot
Match: Q9FNI6 (DNA repair protein RAD5A OS=Arabidopsis thaliana OX=3702 GN=RAD5A PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.4e-43
Identity = 142/517 (27.47%), Postives = 211/517 (40.81%), Query Frame = 0

Query: 371 LLPKLRPYQRRAAYWMVQREKGFSGNSGLGENVQLISPLCLALKCL--DTCLRVFYNPFS 430
           LL +LRPYQ++A +WM Q EKG    +   E   ++ P C    CL     L V+ N F+
Sbjct: 344 LLCELRPYQKQALHWMTQLEKG----NCTDEAATMLHP-CWEAYCLADKRELVVYLNSFT 403

Query: 431 GNLTLNAEQILPHVYGGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQ 490
           G+ T++    L    GGILAD MGLGKT+  ++ +L+H         GF           
Sbjct: 404 GDATIHFPSTLQMARGGILADAMGLGKTVMTISLLLAHSWKA--ASTGF----------- 463

Query: 491 RTEFKRLKRERVECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTED 550
                                            +C  ++    +  S       P+    
Sbjct: 464 ---------------------------------LCPNYEGDKVISSSVDDLTSPPVKATK 523

Query: 551 GNSREKRNNRSTLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL 610
               +KR                       +    S +  G  LIVCP  +L QW+ EI 
Sbjct: 524 FLGFDKR-----------------------LLEQKSVLQNGGNLIVCPMTLLGQWKTEIE 583

Query: 611 RHTRPGSIKLLVYEG---VRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHD-SDRH 670
            H +PGS+ + V+ G    +D  LL            SD+V+T+Y VL  + S + S  H
Sbjct: 584 MHAKPGSLSVYVHYGQSRPKDAKLLS----------QSDVVITTYGVLTSEFSQENSADH 643

Query: 671 EGDRRFMRFQKRYPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITG 730
           EG                 +  + W+RI LDE   ++++ +  +  A+ L A   WC+TG
Sbjct: 644 EG-----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALVADRRWCLTG 703

Query: 731 TPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK 790
           TPIQ  LEDLY LLRF++  P+    WW ++++ P+E  D   ++      K IM R  K
Sbjct: 704 TPIQNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSILKPIMLRRTK 736

Query: 791 IHVTDE----LQLPPQEEQVTWLKFSPIEEHFYQRQHE-SCVSYAREVIQGLKDDFVKRK 850
                E    L LPP + +V + + S  E  FY    + S V + + V QG         
Sbjct: 764 SSTDREGRPILVLPPADARVIYCELSESERDFYDALFKRSKVKFDQFVEQG--------- 736

Query: 851 VPDCVSSDVPSDLLITHADAGKLLSTLLKLRQACCHP 877
                         + H +   +L  LL+LRQ C HP
Sbjct: 824 -------------KVLH-NYASILELLLRLRQCCDHP 736

HSP 2 Score: 107.8 bits (268), Expect = 1.2e-21
Identity = 80/311 (25.72%), Postives = 143/311 (45.98%), Query Frame = 0

Query: 1426 SSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQEKLSNQKMVFQCGHITCCKCLFAM 1485
            SS L    +   +   +++  E   K ++  CP+C E L +  ++  C H  C +CL A 
Sbjct: 763  SSGLEREGKDVPSEAFVQEVVEELRKGEQGECPICLEALED-AVLTPCAHRLCRECLLAS 822

Query: 1486 TEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTLLETSREHEMSITVQG 1545
               +  G        +CP CR                 T+    L+    E    + V+ 
Sbjct: 823  WRNSTSG--------LCPVCR----------------NTVSKQELITAPTESRFQVDVEK 882

Query: 1546 SY--GTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKS 1605
            ++   +KI A++  +  ++   S +K ++FS W   LD+LQ   + NN +F+R+ G    
Sbjct: 883  NWVESSKITALLEELEGLR--SSGSKSILFSQWTAFLDLLQIPLSRNNFSFVRLDG---- 942

Query: 1606 QTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPL 1665
                           + +++++     E  S  VLL+ ++ G  G+NL  A +  +++P 
Sbjct: 943  ------------TLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPW 1002

Query: 1666 LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYKLNRSRESSSFITGNAKNQDQP 1725
             NPA E QA+ R+HRIGQ  +  + RFIVK TVEE +  +   ++    I+G   +Q+  
Sbjct: 1003 WNPAVEEQAVMRIHRIGQTKEVKIRRFIVKGTVEERMEAVQARKQ--RMISGALTDQEVR 1028

Query: 1726 CLTLKEVESLF 1735
               ++E++ LF
Sbjct: 1063 SARIEELKMLF 1028

BLAST of PI0009324 vs. ExPASy Swiss-Prot
Match: Q9FIY7 (DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.9e-39
Identity = 141/522 (27.01%), Postives = 208/522 (39.85%), Query Frame = 0

Query: 375  LRPYQRRAAYWMVQREKGFSGNSGLGENVQLISPLCLALK-CLDTCLRVFYNPFSGNLTL 434
            LRPYQ++A YWM + EKG      + +  + + P   A + C +    ++ N FSG  T+
Sbjct: 616  LRPYQKQALYWMSESEKGID----VEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675

Query: 435  NAEQILPHVYGGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFK 494
                      GGILAD MGLGKT+  +A IL+        G+G       LV D      
Sbjct: 676  QFPTATQMARGGILADAMGLGKTVMTIALILARP------GRGNPENEDVLVADV----- 735

Query: 495  RLKRERVECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNSRE 554
                                                                     + +
Sbjct: 736  ---------------------------------------------------------NAD 795

Query: 555  KRNNRSTLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRP 614
            KRN +                  E+  A  +  A G TLI+CP  +L QW+ E+  H++P
Sbjct: 796  KRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSKP 855

Query: 615  GSIKLLVYEGVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRF 674
             ++ +LVY G       G  +     + + D+VLT+Y VL      D             
Sbjct: 856  DTVSVLVYYG-------GDRTHDAKAIASHDVVLTTYGVLTSAYKQD------------- 915

Query: 675  QKRYPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLED 734
                 +  +   RI W+RI LDE   ++S  T A +    L +   WC+TGTP+Q KLED
Sbjct: 916  -----MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLED 975

Query: 735  LYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDE--- 794
            LY LL F+   P+    WW ++I+ PYE  DP  ++      + +M R  K     E   
Sbjct: 976  LYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGSL 1000

Query: 795  -LQLPPQEEQVTWLKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVP 854
             L+LPP + QV   + S  E  FY         + R  +Q   D FV +           
Sbjct: 1036 ILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFVAQ----------- 1000

Query: 855  SDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM 892
              +L  +A+   +L  LL+LRQ C HP +  S   S Q + +
Sbjct: 1096 GKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADSQQYADL 1000

HSP 2 Score: 101.7 bits (252), Expect = 8.9e-20
Identity = 88/326 (26.99%), Postives = 143/326 (43.87%), Query Frame = 0

Query: 1417 SKQTIPLDSSSDLALTQEP------AITSTAMEQKNENTLKADEESCPVCQEKLSNQKMV 1476
            S+Q   LDS +   L   P      A +   +E+  ++    + + CP+C E  ++  ++
Sbjct: 994  SQQYADLDSLARRFLDNNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLES-ADDPVL 1053

Query: 1477 FQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1536
              C H  C +CL         G        +CP CR                  L  + L
Sbjct: 1054 TPCAHRMCRECLLTSWRSPSCG--------LCPICR----------------TILKRTEL 1113

Query: 1537 LETSREHEMSITVQGSY--GTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFA 1596
            +    +    + V  ++   +K+  +++ +  IK + S  K +VFS W   LD+L+    
Sbjct: 1114 ISCPTDSIFRVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLR 1173

Query: 1597 ANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLIQHGANG 1656
                 F+R  G              K+  K  +K     N  E +   +LL+ ++ G  G
Sbjct: 1174 RRGFEFLRFDG--------------KLAQKGREKVLKEFN--ETKQKTILLMSLKAGGVG 1233

Query: 1657 LNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYKLNRSRE 1716
            LNL  A  V L++P  NPA E QAI R+HRIGQ+   FV RFIVKDTVEE + ++   ++
Sbjct: 1234 LNLTAASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQ 1276

Query: 1717 SSSFITGNAKNQDQPCLTLKEVESLF 1735
                I G   +++     L+E++ LF
Sbjct: 1294 --RMIAGALTDEEVRSARLEELKMLF 1276

BLAST of PI0009324 vs. ExPASy TrEMBL
Match: A0A0A0K8S1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014630 PE=3 SV=1)

HSP 1 Score: 3308.5 bits (8577), Expect = 0.0e+00
Identity = 1675/1790 (93.58%), Postives = 1706/1790 (95.31%), Query Frame = 0

Query: 10   FFSIMVAN-----CLSLTQNDVSNAEWKLYPLASCISSNPA------------------- 69
            F S++V N      LSLTQNDVSNAEWKLY LASCISSNPA                   
Sbjct: 4    FGSLIVRNDRSKPSLSLTQNDVSNAEWKLYSLASCISSNPAVYPQCIFALISLKFHHQHK 63

Query: 70   NFRLLHHGCAPIITLFASQNFILLSGDFLLTLYTCFSGMGRRKQKQPHRSGGIRFEDRGD 129
            NF LLHHGCAPI TLFASQ+FILLSGDFL  +Y  F  MGRRKQKQP+RSGGIRFEDRGD
Sbjct: 64   NFWLLHHGCAPINTLFASQSFILLSGDFLDFIY-LFLRMGRRKQKQPNRSGGIRFEDRGD 123

Query: 130  PKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGWYSTEHHDISEVFLTDVRLEHPF 189
             KTQLDIN VVETA ESSDIKLD VNEPIFVEVDRSGWYS EH+DISEVFL DVRLEHPF
Sbjct: 124  HKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGWYSNEHYDISEVFLADVRLEHPF 183

Query: 190  VSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWPVLSSNDIFLELIERDMEEDVKA 249
            V FCLDKSV ENSRYSLRFRLCNVNGSL+DRIKFGHWPVLSSND FLE IERDMEEDVKA
Sbjct: 184  VGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWPVLSSNDTFLEFIERDMEEDVKA 243

Query: 250  CSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISFSQNTGSFRLRVEILSSAFDACE 309
            CSVVLSGN DGPD+AISGLVHLANLKLMTLRPVDG+ FSQN GS RLRVEILSSAFDACE
Sbjct: 244  CSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPFSQNMGSLRLRVEILSSAFDACE 303

Query: 310  SIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKSTNTDTHLHHEVGDDTSNSRKRVN 369
            SIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+N DTHLHHE GDDTSNSRK  N
Sbjct: 304  SIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSSNMDTHLHHEAGDDTSNSRKHAN 363

Query: 370  FDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAAYWMVQREKGFSGNSGLGENVQL 429
            FDT GFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAAYWM+QREKGFSGNSGLGENVQL
Sbjct: 364  FDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAAYWMIQREKGFSGNSGLGENVQL 423

Query: 430  ISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVYGGILADEMGLGKTIELLACILS 489
            ISPLC+ALKCLDTCLRVFYNPFSGNLTLNAEQ+LPHVYGGILADEMGLGKTIELLACILS
Sbjct: 424  ISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVYGGILADEMGLGKTIELLACILS 483

Query: 490  HQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECLCGAVSENYRYKGLWVQCDICDA 549
            HQMSVFEG KGFDVEVQQLV+DQRTEFKRLKRER+ECLCGAVSENYRYKGLWVQCDICDA
Sbjct: 484  HQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECLCGAVSENYRYKGLWVQCDICDA 543

Query: 550  WQHADCVGYSPKGRIVKPIDTEDGNS-REKRNNRSTLNVTVRAEEHVCTPCLELMQATDS 609
            WQHADCVGYSPKGRIVK +DTEDGNS REKRNNR+TLNV VRAEEHVCTPCLELMQATDS
Sbjct: 544  WQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLNVIVRAEEHVCTPCLELMQATDS 603

Query: 610  PMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYEGVRDTSLLGTLSVQINELINSD 669
            PMATGATLIVCPAPILFQWQAEILRHT PGS+KLLVYEGVRDTSLLGTLS QIN+LINSD
Sbjct: 604  PMATGATLIVCPAPILFQWQAEILRHTHPGSMKLLVYEGVRDTSLLGTLSAQINDLINSD 663

Query: 670  IVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPTPLTRIFWWRICLDEGQMVESNA 729
            IVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPV+PTPLTRIFWWRICLDEGQMVESNA
Sbjct: 664  IVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPTPLTRIFWWRICLDEGQMVESNA 723

Query: 730  TAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRD 789
            TAATEMASRLYASH WCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRD
Sbjct: 724  TAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRD 783

Query: 790  PGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWLKFSPIEEHFYQRQHESCVSYA 849
            PGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWLKFSPIEEHFYQRQHE+CVSYA
Sbjct: 784  PGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTWLKFSPIEEHFYQRQHETCVSYA 843

Query: 850  REVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLR 909
            REVIQGLKDDFVKRKVPDCV+SD+PSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLR
Sbjct: 844  REVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLR 903

Query: 910  SLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNALAGIAIIEKKFSEAFSLYKEALE 969
            SLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNALAGIAIIEKKFSEAFSLYKEALE
Sbjct: 904  SLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNALAGIAIIEKKFSEAFSLYKEALE 963

Query: 970  LAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPLKDQFYPRTCEVKASRMDDSEKY 1029
             AEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPLKDQ  PRTCEVKASRMDDSEKY
Sbjct: 964  FAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPLKDQLCPRTCEVKASRMDDSEKY 1023

Query: 1030 DDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGKDTNAKKEENYEPHRSSSYFDEI 1089
            DDHVHIMKKQKVSETLYA CSEDNTGKMIDHPLQL GKDTNAKKEENYEPHRSS YFDEI
Sbjct: 1024 DDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGKDTNAKKEENYEPHRSSGYFDEI 1083

Query: 1090 SVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSELKDRENLNDVWWLEAVHHAEQN 1149
            SVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQV SELKDREN NDVWWLEAVHHAEQN
Sbjct: 1084 SVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSELKDRENFNDVWWLEAVHHAEQN 1143

Query: 1150 KDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKYHVQSGLDLLEASRKVVLDRLLE 1209
            KDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKYHVQSGLDLLEASRKVVLDRLLE
Sbjct: 1144 KDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKYHVQSGLDLLEASRKVVLDRLLE 1203

Query: 1210 IDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDELFQEYEARLFRLNKVQGGMVTS 1269
            IDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELDELFQEYEARLFRLNKVQGGMVTS
Sbjct: 1204 IDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDELFQEYEARLFRLNKVQGGMVTS 1263

Query: 1270 VEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEPNKRDVGEKVMVSKHPSELEVVL 1329
            VEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEPNKRD GEKVMVSKHPSELEVVL
Sbjct: 1264 VEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEPNKRDAGEKVMVSKHPSELEVVL 1323

Query: 1330 GVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRL 1389
            GVIKNFCKTQLGKESI AANKQLHLLE+MRKEYGHARSLAIAQAQVLNAHDEIKMATTRL
Sbjct: 1324 GVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARSLAIAQAQVLNAHDEIKMATTRL 1383

Query: 1390 CLR-DNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPL 1449
             LR D+DDDSSA+ ISEHELPAASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPL
Sbjct: 1384 SLRGDDDDDSSAFTISEHELPAASVQYSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPL 1443

Query: 1450 DSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQEKLSNQKMVFQCGHITCCKCLF 1509
            DSSSDLALTQEPAITSTA EQKNENT KADEESCPVCQEKL+NQKMVFQCGHITCCKCLF
Sbjct: 1444 DSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQEKLNNQKMVFQCGHITCCKCLF 1503

Query: 1510 AMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTLLETSREHEMSITV 1569
            AMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPST LETSREHEMSITV
Sbjct: 1504 AMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTSLETSREHEMSITV 1563

Query: 1570 QGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKS 1629
            QGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVL YAF ANNITFIRMKGGRKS
Sbjct: 1564 QGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLHYAFVANNITFIRMKGGRKS 1623

Query: 1630 QTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPL 1689
            QTAISEFRGQKINAKENKKKRLST+LPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPL
Sbjct: 1624 QTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPL 1683

Query: 1690 LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYKLNRSRESSSFITGNAKNQDQP 1749
            LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYKLNRSRESSSFITGNAKNQDQP
Sbjct: 1684 LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYKLNRSRESSSFITGNAKNQDQP 1743

Query: 1750 CLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAATIAAERRLREHT 1774
             LTLKEVESLFASKA PLGENDDKGSETLRQLAPSMAATIAAERRLREHT
Sbjct: 1744 FLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAATIAAERRLREHT 1792

BLAST of PI0009324 vs. ExPASy TrEMBL
Match: A0A1S3C3N1 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)

HSP 1 Score: 3244.1 bits (8410), Expect = 0.0e+00
Identity = 1626/1691 (96.16%), Postives = 1658/1691 (98.05%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQKQPH SGGIRFEDRGDPKTQLDINGV ETAAE+SDIKLDEVNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
            YS EH+DISEVFL DVRLE+PFV FCLDKS+ ENSRYSLRFRLCNVNGSL+DRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSNDIFLELIERD+EEDVKACSVVLSGNFDGPD+AISGLVHLANLKLMTLRPVDG+SF
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQNTGS RLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N DTHLHHE GDD SNSRK VNFDT  FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWMVQREKGFSGNSGLGENVQLISPLC+ALKCL+TCLRVFYNPFSGNLTLNAEQILPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEV+QLV+DQRTEFKRLKRERVECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNS-REKRNNRSTLN 563
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKP+DTEDGNS REKRNN++TLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            V VRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
            GVRDTSLLGTLSVQIN+LINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPV+PT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            LKFSP+EEHFYQRQHE+CVSYAREVIQGLKDDFVKRKVPDCVSSD+PSDLL+THADAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAII KKF EAFSLYKEALELAEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQF PRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYA CSEDNTGKMIDHPLQLTGK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            DTNAKKEENYEPHRS+SYFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQV SE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1403
            LAIAQA VLNAHDEIKMATTRLCLRDNDDDSSA++ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1404 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQE 1463
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTAMEQKNENTLKADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1464 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1523
            KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1524 ETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1583
            ETLDPSTLLETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1584 LQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLI 1643
            L YAFAANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLST+LPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1644 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1703
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1704 LNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAAT 1763
            LNRSRESSSFITGNAKNQDQP LTLKEVESLFASKA PLGENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1680

Query: 1764 IAAERRLREHT 1774
            IAAERRLREHT
Sbjct: 1681 IAAERRLREHT 1691

BLAST of PI0009324 vs. ExPASy TrEMBL
Match: A0A1S3C421 (E3 ubiquitin-protein ligase SHPRH isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)

HSP 1 Score: 3142.8 bits (8147), Expect = 0.0e+00
Identity = 1587/1691 (93.85%), Postives = 1617/1691 (95.62%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQKQPH SGGIRFEDRGDPKTQLDINGV ETAAE+SDIKLDEVNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
            YS EH+DISEVFL DVRLE+PFV FCLDKS+ ENSRYSLRFRLCNVNGSL+DRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSNDIFLELIERD+EEDVKACSVVLSGNFDGPD+AISGLVHLANLKLMTLRPVDG+SF
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQNTGS RLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N DTHLHHE GDD SNSRK VNFDT  FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWMVQREKGFSGNSGLGENVQLISPLC+ALKCL+TCLRVFYNPFSGNLTLNAEQILPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEV+QLV+DQRTEFKRLKRERVECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNS-REKRNNRSTLN 563
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKP+DTEDGNS REKRNN++TLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            V VRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
            GVRDTSLLGTLSVQIN+LINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPV+PT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            LKFSP+EEHFYQRQHE+C                                         L
Sbjct: 721  LKFSPVEEHFYQRQHETC-----------------------------------------L 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAII KKF EAFSLYKEALELAEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQF PRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYA CSEDNTGKMIDHPLQLTGK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            DTNAKKEENYEPHRS+SYFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQV SE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1403
            LAIAQA VLNAHDEIKMATTRLCLRDNDDDSSA++ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1404 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQE 1463
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTAMEQKNENTLKADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1464 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1523
            KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1524 ETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1583
            ETLDPSTLLETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1584 LQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLI 1643
            L YAFAANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLST+LPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1644 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1703
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1704 LNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAAT 1763
            LNRSRESSSFITGNAKNQDQP LTLKEVESLFASKA PLGENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1650

Query: 1764 IAAERRLREHT 1774
            IAAERRLREHT
Sbjct: 1681 IAAERRLREHT 1650

BLAST of PI0009324 vs. ExPASy TrEMBL
Match: A0A1S3C4R3 (E3 ubiquitin-protein ligase SHPRH isoform X3 OS=Cucumis melo OX=3656 GN=LOC103496631 PE=3 SV=1)

HSP 1 Score: 3084.7 bits (7996), Expect = 0.0e+00
Identity = 1560/1691 (92.25%), Postives = 1591/1691 (94.09%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQKQPH SGGIRFEDRGDPKTQLDINGV ETAAE+SDIKLDEVNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
            YS EH+DISEVFL DVRLE+PFV FCLDKS+ ENSRYSLRFRLCNVNGSL+DRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSNDIFLELIERD+EEDVKACSVVLSGNFDGPD+AISGLVHLANLKLMTLRPVDG+SF
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQNTGS RLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N DTHLHHE GDD SNSRK VNFDT  FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWMVQREKGFSGNSGLGENVQLISPLC+ALKCL+TCLRVFYNPFSGNLTLNAEQILPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEV+QLV+DQRTEFKRLKRERVECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNS-REKRNNRSTLN 563
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKP+DTEDGNS REKRNN++TLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            V VRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL              
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL-------------- 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
                                                                 RYPV+PT
Sbjct: 541  -----------------------------------------------------RYPVIPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            LKFSP+EEHFYQRQHE+CVSYAREVIQGLKDDFVKRKVPDCVSSD+PSDLL+THADAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAII KKF EAFSLYKEALELAEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQF PRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYA CSEDNTGKMIDHPLQLTGK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            DTNAKKEENYEPHRS+SYFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQV SE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1403
            LAIAQA VLNAHDEIKMATTRLCLRDNDDDSSA++ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1404 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQE 1463
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTAMEQKNENTLKADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1464 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1523
            KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1524 ETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1583
            ETLDPSTLLETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1584 LQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLI 1643
            L YAFAANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLST+LPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1644 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1703
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1704 LNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAAT 1763
            LNRSRESSSFITGNAKNQDQP LTLKEVESLFASKA PLGENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1624

Query: 1764 IAAERRLREHT 1774
            IAAERRLREHT
Sbjct: 1681 IAAERRLREHT 1624

BLAST of PI0009324 vs. ExPASy TrEMBL
Match: A0A6J1FE16 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)

HSP 1 Score: 2929.4 bits (7593), Expect = 0.0e+00
Identity = 1472/1691 (87.05%), Postives = 1568/1691 (92.73%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQKQPHRSGGIRFED   PKTQ+DING+VET  ESSDIKLDEVNEP+FVEVDR+GW
Sbjct: 1    MGRRKQKQPHRSGGIRFEDPVSPKTQVDINGIVET-VESSDIKLDEVNEPLFVEVDRTGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
            +S EHHDISEV L D+RLEHPFV    ++  CE+SRYSLRFRLCN NG L+DRIKFGHWP
Sbjct: 61   HSNEHHDISEVVLMDIRLEHPFVGIQSEEIFCESSRYSLRFRLCNDNGFLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSNDI LELIER +EED+  CSVVLSG+FDGPD+AISGLVHLA+LK +TLRPV G+SF
Sbjct: 121  VLSSNDISLELIERRVEEDMNGCSVVLSGSFDGPDEAISGLVHLAHLKFVTLRPVMGVSF 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQN GS RLRVEILSSAFDAC SI D+GRQLWKKSMMNTIAWLRPEVV SEVKYGVV ST
Sbjct: 181  SQNMGSLRLRVEILSSAFDACGSILDSGRQLWKKSMMNTIAWLRPEVVSSEVKYGVVGST 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N D HLH E GDD S+SRK VNFD+  FYDAIKPSKD+PMLDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDAHLHRETGDDASSSRKHVNFDSVAFYDAIKPSKDDPMLDEDIPNLLPKLRPYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWMVQREKGFS N G+GENVQLISPLC+ L+CLDT  R+FYNPFSG+LT NAEQILPHVY
Sbjct: 301  YWMVQREKGFSRNFGVGENVQLISPLCMPLECLDTRSRMFYNPFSGDLTWNAEQILPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSH+MSVFE GKG D E+Q LV DQRT+FKRLKRERVEC+
Sbjct: 361  GGILADEMGLGKTIELLACILSHRMSVFESGKGLDDELQPLVGDQRTKFKRLKRERVECV 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNSR-EKRNNRSTLN 563
            CGAVSEN+RY+GLWVQCDICDAWQHADCVGYSPKGRI++P+DT+DGN R +KRN R+T+N
Sbjct: 421  CGAVSENHRYEGLWVQCDICDAWQHADCVGYSPKGRILEPVDTKDGNCRKDKRNKRNTVN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            VTVR EEHVC PCLELMQATDSPM TGATLIVCPAPILFQW AEILRHT PGSIKLLVYE
Sbjct: 481  VTVRDEEHVCMPCLELMQATDSPMTTGATLIVCPAPILFQWHAEILRHTHPGSIKLLVYE 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
            GVRDTSL  T  VQIN++INSDIVLTSYDVLKEDLSHD DRHEGDRRFMRFQKRYPV+PT
Sbjct: 541  GVRDTSLSNTSPVQINDIINSDIVLTSYDVLKEDLSHDYDRHEGDRRFMRFQKRYPVVPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRFVK
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK+HVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKVHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            L FSPIEE+FYQRQHE+CVSYAREVIQGLKDDFVKRKVP  VSSDVPSD+LITHADAGKL
Sbjct: 721  LTFSPIEENFYQRQHETCVSYAREVIQGLKDDFVKRKVPGFVSSDVPSDILITHADAGKL 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            L+TLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRR+VVALNAL
Sbjct: 781  LNTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRTVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAIIEKKFSEA SLYKEALELAEENNEDFR+DPLLSIHIHHNLAEILPLA++Q QSPL
Sbjct: 841  AGIAIIEKKFSEALSLYKEALELAEENNEDFRVDPLLSIHIHHNLAEILPLAMDQIQSPL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQFYPRTCEVKAS+MDDSEK D+  HI+KKQKV+ TLYAPCS+  T K+ID PL+LTG 
Sbjct: 901  KDQFYPRTCEVKASKMDDSEKSDN--HIIKKQKVNGTLYAPCSD--TEKIIDSPLELTGL 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            D NAKKEENYEPHRSSSYF++  +RK CE MRQKYLAVFSSKL IAQQEFTKSY+QV +E
Sbjct: 961  DENAKKEENYEPHRSSSYFNDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVCNE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LK RENLN+VWWL+AVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSI ALKY
Sbjct: 1021 LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQ+GLDLLEASRK+V+ RLLEIDQTME+PK+EDIERVRYC+NCQADSNGPPCVLCELDE
Sbjct: 1081 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLN VQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKN SSS+ G+EE 
Sbjct: 1141 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRD GE+V+VSKHPSELEVVLGVIKNFCKTQLGKESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1403
            LAIAQAQVLNAHDEIKMATTRLCLR NDDD SAYAISEHELPAASVQYSS+KF+SLA LS
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1320

Query: 1404 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQE 1463
            RVKGKLRYLKGLVQSKQ IPL SSS+ A TQ P ITST++EQK+ENTLKADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQE 1380

Query: 1464 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1523
             LSNQKMVFQCGH+TCCKCLFAMTEK L  S IQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 TLSNQKMVFQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1524 ETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1583
            ETLDPSTL +TSRE E SI VQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1584 LQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLI 1643
            LQYAFAANNITF+RMKGGRKS TAISEFRGQKINAKEN KK+    +PE+RSAQVLLLLI
Sbjct: 1501 LQYAFAANNITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLI 1560

Query: 1644 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1703
            QHGANGLNLLEAQHV+LVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESI++
Sbjct: 1561 QHGANGLNLLEAQHVILVEPLLNPAAEAQAISRVHRIGQQNKTFVHRFIVKDTVEESIHR 1620

Query: 1704 LNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAAT 1763
            LNRSRESSSFITGN KNQDQP  TLKEVESLFAS+  PL E+D+K SETLR LAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPVFTLKEVESLFASRPSPLRESDEKESETLRHLAPSMAAT 1680

Query: 1764 IAAERRLREHT 1774
            IAAERRL+EHT
Sbjct: 1681 IAAERRLKEHT 1686

BLAST of PI0009324 vs. NCBI nr
Match: XP_008456785.1 (PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo] >XP_008456786.1 PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo] >XP_008456787.1 PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo] >XP_016901994.1 PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis melo])

HSP 1 Score: 3244.1 bits (8410), Expect = 0.0e+00
Identity = 1626/1691 (96.16%), Postives = 1658/1691 (98.05%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQKQPH SGGIRFEDRGDPKTQLDINGV ETAAE+SDIKLDEVNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
            YS EH+DISEVFL DVRLE+PFV FCLDKS+ ENSRYSLRFRLCNVNGSL+DRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSNDIFLELIERD+EEDVKACSVVLSGNFDGPD+AISGLVHLANLKLMTLRPVDG+SF
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQNTGS RLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N DTHLHHE GDD SNSRK VNFDT  FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWMVQREKGFSGNSGLGENVQLISPLC+ALKCL+TCLRVFYNPFSGNLTLNAEQILPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEV+QLV+DQRTEFKRLKRERVECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNS-REKRNNRSTLN 563
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKP+DTEDGNS REKRNN++TLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            V VRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
            GVRDTSLLGTLSVQIN+LINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPV+PT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            LKFSP+EEHFYQRQHE+CVSYAREVIQGLKDDFVKRKVPDCVSSD+PSDLL+THADAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAII KKF EAFSLYKEALELAEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQF PRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYA CSEDNTGKMIDHPLQLTGK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            DTNAKKEENYEPHRS+SYFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQV SE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1403
            LAIAQA VLNAHDEIKMATTRLCLRDNDDDSSA++ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1404 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQE 1463
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTAMEQKNENTLKADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1464 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1523
            KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1524 ETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1583
            ETLDPSTLLETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1584 LQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLI 1643
            L YAFAANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLST+LPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1644 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1703
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1704 LNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAAT 1763
            LNRSRESSSFITGNAKNQDQP LTLKEVESLFASKA PLGENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1680

Query: 1764 IAAERRLREHT 1774
            IAAERRLREHT
Sbjct: 1681 IAAERRLREHT 1691

BLAST of PI0009324 vs. NCBI nr
Match: XP_011656441.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Cucumis sativus] >KGN45843.2 hypothetical protein Csa_005571 [Cucumis sativus])

HSP 1 Score: 3221.0 bits (8350), Expect = 0.0e+00
Identity = 1617/1692 (95.57%), Postives = 1644/1692 (97.16%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQKQP+RSGGIRFEDRGD KTQLDIN VVETA ESSDIKLD VNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPNRSGGIRFEDRGDHKTQLDINEVVETAVESSDIKLDGVNEPIFVEVDRSGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
            YS EH+DISEVFL DVRLEHPFV FCLDKSV ENSRYSLRFRLCNVNGSL+DRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLADVRLEHPFVGFCLDKSVRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSND FLE IERDMEEDVKACSVVLSGN DGPD+AISGLVHLANLKLMTLRPVDG+ F
Sbjct: 121  VLSSNDTFLEFIERDMEEDVKACSVVLSGNLDGPDEAISGLVHLANLKLMTLRPVDGVPF 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQN GS RLRVEILSSAFDACESIFDNGRQLWKKSMMNTI WLRPEVVLSEVKYGVVKS+
Sbjct: 181  SQNMGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTITWLRPEVVLSEVKYGVVKSS 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N DTHLHHE GDDTSNSRK  NFDT GFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEAGDDTSNSRKHANFDTIGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWM+QREKGFSGNSGLGENVQLISPLC+ALKCLDTCLRVFYNPFSGNLTLNAEQ+LPHVY
Sbjct: 301  YWMIQREKGFSGNSGLGENVQLISPLCMALKCLDTCLRVFYNPFSGNLTLNAEQVLPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSHQMSVFEG KGFDVEVQQLV+DQRTEFKRLKRER+ECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGRKGFDVEVQQLVEDQRTEFKRLKRERIECL 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNS-REKRNNRSTLN 563
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVK +DTEDGNS REKRNNR+TLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKSVDTEDGNSRREKRNNRNTLN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            V VRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHT PGS+KLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSMKLLVYE 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
            GVRDTSLLGTLS QIN+LINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPV+PT
Sbjct: 541  GVRDTSLLGTLSAQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRFVK
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            LKFSPIEEHFYQRQHE+CVSYAREVIQGLKDDFVKRKVPDCV+SD+PSDLLITHADAGKL
Sbjct: 721  LKFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVTSDIPSDLLITHADAGKL 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAIIEKKFSEAFSLYKEALE AEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL
Sbjct: 841  AGIAIIEKKFSEAFSLYKEALEFAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQ  PRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYA CSEDNTGKMIDHPLQL GK
Sbjct: 901  KDQLCPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLKGK 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            DTNAKKEENYEPHRSS YFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQV SE
Sbjct: 961  DTNAKKEENYEPHRSSGYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LKDREN NDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENFNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQSGLDLLEASRKVVLDRLLEIDQTM++PKEEDIERVRYCR CQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMKSPKEEDIERVRYCRICQADSNGPPCVLCELDE 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRD GEKVMVSKHPSELEVVLGVIKNFCKTQLGKESI AANKQLHLLE+MRKEYGHARS
Sbjct: 1201 NKRDAGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEHMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLR-DNDDDSSAYAISEHELPAASVQYSSDKFMSLAML 1403
            LAIAQAQVLNAHDEIKMATTRL LR D+DDDSSA+ ISEHELPAASVQYSSDKFMSLAML
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLSLRGDDDDDSSAFTISEHELPAASVQYSSDKFMSLAML 1320

Query: 1404 SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQ 1463
            SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTA EQKNENT KADEESCPVCQ
Sbjct: 1321 SRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTATEQKNENTSKADEESCPVCQ 1380

Query: 1464 EKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK 1523
            EKL+NQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK
Sbjct: 1381 EKLNNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSK 1440

Query: 1524 NETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD 1583
            NETLDPST LETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD
Sbjct: 1441 NETLDPSTSLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLD 1500

Query: 1584 VLQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLL 1643
            VL YAF ANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLST+LPELRSAQVLLLL
Sbjct: 1501 VLHYAFVANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLL 1560

Query: 1644 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIY 1703
            IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIY
Sbjct: 1561 IQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIY 1620

Query: 1704 KLNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAA 1763
            KLNRSRESSSFITGNAKNQDQP LTLKEVESLFASKA PLGENDDKGSETLRQLAPSMAA
Sbjct: 1621 KLNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAA 1680

Query: 1764 TIAAERRLREHT 1774
            TIAAERRLREHT
Sbjct: 1681 TIAAERRLREHT 1692

BLAST of PI0009324 vs. NCBI nr
Match: XP_008456789.1 (PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X2 [Cucumis melo])

HSP 1 Score: 3142.8 bits (8147), Expect = 0.0e+00
Identity = 1587/1691 (93.85%), Postives = 1617/1691 (95.62%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQKQPH SGGIRFEDRGDPKTQLDINGV ETAAE+SDIKLDEVNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
            YS EH+DISEVFL DVRLE+PFV FCLDKS+ ENSRYSLRFRLCNVNGSL+DRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSNDIFLELIERD+EEDVKACSVVLSGNFDGPD+AISGLVHLANLKLMTLRPVDG+SF
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQNTGS RLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N DTHLHHE GDD SNSRK VNFDT  FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWMVQREKGFSGNSGLGENVQLISPLC+ALKCL+TCLRVFYNPFSGNLTLNAEQILPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEV+QLV+DQRTEFKRLKRERVECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNS-REKRNNRSTLN 563
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKP+DTEDGNS REKRNN++TLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            V VRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
            GVRDTSLLGTLSVQIN+LINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPV+PT
Sbjct: 541  GVRDTSLLGTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            LKFSP+EEHFYQRQHE+C                                         L
Sbjct: 721  LKFSPVEEHFYQRQHETC-----------------------------------------L 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAII KKF EAFSLYKEALELAEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQF PRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYA CSEDNTGKMIDHPLQLTGK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            DTNAKKEENYEPHRS+SYFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQV SE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1403
            LAIAQA VLNAHDEIKMATTRLCLRDNDDDSSA++ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1404 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQE 1463
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTAMEQKNENTLKADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1464 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1523
            KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1524 ETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1583
            ETLDPSTLLETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1584 LQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLI 1643
            L YAFAANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLST+LPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1644 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1703
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1704 LNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAAT 1763
            LNRSRESSSFITGNAKNQDQP LTLKEVESLFASKA PLGENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1650

Query: 1764 IAAERRLREHT 1774
            IAAERRLREHT
Sbjct: 1681 IAAERRLREHT 1650

BLAST of PI0009324 vs. NCBI nr
Match: XP_038884903.1 (E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida] >XP_038884904.1 E3 ubiquitin-protein ligase SHPRH isoform X1 [Benincasa hispida])

HSP 1 Score: 3124.0 bits (8098), Expect = 0.0e+00
Identity = 1568/1691 (92.73%), Postives = 1620/1691 (95.80%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQ+QPHRSGGIRFEDRGDPKTQ+DING    A ESSDIK+ EVNEPIFVEVDR+GW
Sbjct: 1    MGRRKQRQPHRSGGIRFEDRGDPKTQIDING----AVESSDIKVAEVNEPIFVEVDRTGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
             S EHHDISEVFL DVRLEHPFV F LDKS CENSRYSLRFRLCNVNGSL+DRIKFGHWP
Sbjct: 61   NSNEHHDISEVFLMDVRLEHPFVDFRLDKSFCENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSNDI LELIERDMEED+KACSVVLSGNFDGPD+AISGLVHLANLK  TLRPV+G+S 
Sbjct: 121  VLSSNDILLELIERDMEEDMKACSVVLSGNFDGPDEAISGLVHLANLKFATLRPVEGVSV 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQNTGS RLRVEILSSAF ACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST
Sbjct: 181  SQNTGSLRLRVEILSSAFAACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N DT LHH  GDDTSNSRK VNFDT  FYDAIKPSKDEPMLDEDIPNLLPKLR YQRRAA
Sbjct: 241  NMDTSLHHGTGDDTSNSRKHVNFDTVAFYDAIKPSKDEPMLDEDIPNLLPKLRQYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWMVQREKGFSGN G+GENVQLISPLC+ALKC+DTC RVFYNPFSGNLTLNAEQ LPHVY
Sbjct: 301  YWMVQREKGFSGNLGVGENVQLISPLCMALKCVDTCSRVFYNPFSGNLTLNAEQTLPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSHQMSVFEGGKGF  E+QQ V+DQRTEFKRLKRERVECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFHDELQQPVEDQRTEFKRLKRERVECL 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNSR-EKRNNRSTLN 563
            CGAVSEN+RYKGLWVQCDICDAWQHADCVGYSPKGRI+KPIDTE GN R EKRNNR+TLN
Sbjct: 421  CGAVSENHRYKGLWVQCDICDAWQHADCVGYSPKGRILKPIDTEGGNCRKEKRNNRNTLN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            V VRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHT PGSIKLLVYE
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTHPGSIKLLVYE 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
            GVRDTSLL TLSVQIN+LINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPV+PT
Sbjct: 541  GVRDTSLLSTLSVQINDLINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVIPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRFVK
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFVK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            L FSPIEEHFYQRQHE+CVSYAREVIQGLKDDFVK+KVPDC+SSD+PSDLLI HADAGKL
Sbjct: 721  LTFSPIEEHFYQRQHETCVSYAREVIQGLKDDFVKKKVPDCISSDIPSDLLINHADAGKL 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKT+IEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTKIEGEEALRRSVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAV+QSQS L
Sbjct: 841  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVDQSQSRL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQF PRT EVKA+RMDDSEKYD+HVH+MKKQKVSETLYAPCSEDNTGKMID PL+LT K
Sbjct: 901  KDQFCPRTSEVKAARMDDSEKYDNHVHVMKKQKVSETLYAPCSEDNTGKMIDRPLELTRK 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            DTN KKEEN EPH SSSYFD+IS+RK CE MRQKYLAVFSSKLS+AQQEFTKSYMQV SE
Sbjct: 961  DTNTKKEENCEPHLSSSYFDDISIRKACEAMRQKYLAVFSSKLSVAQQEFTKSYMQVGSE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LKDR NLN VWWLEAVHHAEQNKDFSHELIRK+EEAVSGNLNNSKSRVGSRFRSI+ALKY
Sbjct: 1021 LKDRGNLNHVWWLEAVHHAEQNKDFSHELIRKVEEAVSGNLNNSKSRVGSRFRSINALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELD+
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDD 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSS+VG+EEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSRVGYEEP 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1403
            LAIAQAQVLNAHDEIKMATTRLCLRD DDDSSAYAISEHELPAASVQYSSDKFMSLAML+
Sbjct: 1261 LAIAQAQVLNAHDEIKMATTRLCLRD-DDDSSAYAISEHELPAASVQYSSDKFMSLAMLA 1320

Query: 1404 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQE 1463
            RVKGKLRYLKGLVQSKQ IPLDSSS+LALTQ+PA  ST+MEQK+ENT KADEESCPVCQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQNIPLDSSSNLALTQQPATMSTSMEQKSENTSKADEESCPVCQE 1380

Query: 1464 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1523
            KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1524 ETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1583
            ETLDPSTL ETSREHE+SITVQGSYGTK+EAVVRRILWIKYTDS+AKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLHETSREHELSITVQGSYGTKVEAVVRRILWIKYTDSKAKVLVFSSWNDVLDV 1500

Query: 1584 LQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLI 1643
            LQYAFAAN+ITFIRMKGGR+S TAISEFRGQKINAKEN+KKR S  +PELRSAQVLLLLI
Sbjct: 1501 LQYAFAANSITFIRMKGGRQSHTAISEFRGQKINAKENQKKRPSGKVPELRSAQVLLLLI 1560

Query: 1644 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1703
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQ+NKTFVHRFIVKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQKNKTFVHRFIVKDTVEESIYK 1620

Query: 1704 LNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAAT 1763
            LNRSRESSSFITGN KNQDQP LTLKEVESLFASKA PL END+KGSETLR LAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNTKNQDQPLLTLKEVESLFASKASPLRENDEKGSETLRHLAPSMAAT 1680

Query: 1764 IAAERRLREHT 1774
            IAAERRL+ HT
Sbjct: 1681 IAAERRLKVHT 1686

BLAST of PI0009324 vs. NCBI nr
Match: XP_008456790.1 (PREDICTED: E3 ubiquitin-protein ligase SHPRH isoform X3 [Cucumis melo])

HSP 1 Score: 3084.7 bits (7996), Expect = 0.0e+00
Identity = 1560/1691 (92.25%), Postives = 1591/1691 (94.09%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQKQPH SGGIRFEDRGDPKTQLDINGV ETAAE+SDIKLDEVNEPIFVEVDRSGW
Sbjct: 1    MGRRKQKQPHCSGGIRFEDRGDPKTQLDINGVAETAAETSDIKLDEVNEPIFVEVDRSGW 60

Query: 144  YSTEHHDISEVFLTDVRLEHPFVSFCLDKSVCENSRYSLRFRLCNVNGSLVDRIKFGHWP 203
            YS EH+DISEVFL DVRLE+PFV FCLDKS+ ENSRYSLRFRLCNVNGSL+DRIKFGHWP
Sbjct: 61   YSNEHYDISEVFLVDVRLENPFVRFCLDKSIRENSRYSLRFRLCNVNGSLLDRIKFGHWP 120

Query: 204  VLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPVDGISF 263
            VLSSNDIFLELIERD+EEDVKACSVVLSGNFDGPD+AISGLVHLANLKLMTLRPVDG+SF
Sbjct: 121  VLSSNDIFLELIERDIEEDVKACSVVLSGNFDGPDEAISGLVHLANLKLMTLRPVDGVSF 180

Query: 264  SQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 323
            SQNTGS RLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST
Sbjct: 181  SQNTGSLRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYGVVKST 240

Query: 324  NTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPYQRRAA 383
            N DTHLHHE GDD SNSRK VNFDT  FYDAIKPSKDEP LDEDIPNLLPKLRPYQRRAA
Sbjct: 241  NMDTHLHHEPGDDASNSRKHVNFDTISFYDAIKPSKDEPGLDEDIPNLLPKLRPYQRRAA 300

Query: 384  YWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQILPHVY 443
            YWMVQREKGFSGNSGLGENVQLISPLC+ALKCL+TCLRVFYNPFSGNLTLNAEQILPHVY
Sbjct: 301  YWMVQREKGFSGNSGLGENVQLISPLCMALKCLNTCLRVFYNPFSGNLTLNAEQILPHVY 360

Query: 444  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVECL 503
            GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEV+QLV+DQRTEFKRLKRERVECL
Sbjct: 361  GGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVRQLVEDQRTEFKRLKRERVECL 420

Query: 504  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNS-REKRNNRSTLN 563
            CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKP+DTEDGNS REKRNN++TLN
Sbjct: 421  CGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPVDTEDGNSRREKRNNKNTLN 480

Query: 564  VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRPGSIKLLVYE 623
            V VRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL              
Sbjct: 481  VIVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL-------------- 540

Query: 624  GVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRFQKRYPVLPT 683
                                                                 RYPV+PT
Sbjct: 541  -----------------------------------------------------RYPVIPT 600

Query: 684  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVK 743
            PLTRIFWWRICLDEGQMVESNATAATEMASRLYASH WCITGTPIQRKLEDLYGLLRF+K
Sbjct: 601  PLTRIFWWRICLDEGQMVESNATAATEMASRLYASHRWCITGTPIQRKLEDLYGLLRFLK 660

Query: 744  ASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 803
            ASPFNVH+WWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW
Sbjct: 661  ASPFNVHKWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQLPPQEEQVTW 720

Query: 804  LKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL 863
            LKFSP+EEHFYQRQHE+CVSYAREVIQGLKDDFVKRKVPDCVSSD+PSDLL+THADAGKL
Sbjct: 721  LKFSPVEEHFYQRQHETCVSYAREVIQGLKDDFVKRKVPDCVSSDIPSDLLVTHADAGKL 780

Query: 864  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 923
            LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL
Sbjct: 781  LSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEALRRSVVALNAL 840

Query: 924  AGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILPLAVNQSQSPL 983
            AGIAII KKF EAFSLYKEALELAEEN EDFRLDPLLSIHIHHNLAEILPLAVNQSQS L
Sbjct: 841  AGIAIIVKKFFEAFSLYKEALELAEENIEDFRLDPLLSIHIHHNLAEILPLAVNQSQSRL 900

Query: 984  KDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKMIDHPLQLTGK 1043
            KDQF PRTCEVKASRMDDSEKY  HVHIMKKQKVSETLYA CSEDNTGKMIDHPLQLTGK
Sbjct: 901  KDQFCPRTCEVKASRMDDSEKYVGHVHIMKKQKVSETLYATCSEDNTGKMIDHPLQLTGK 960

Query: 1044 DTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVRSE 1103
            DTNAKKEENYEPHRS+SYFDEI VRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQV SE
Sbjct: 961  DTNAKKEENYEPHRSNSYFDEIFVRKVCEVMRQKYLAVFSSKLSIAQQEFTKSYMQVGSE 1020

Query: 1104 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1163
            LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY
Sbjct: 1021 LKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSISALKY 1080

Query: 1164 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1223
            HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE
Sbjct: 1081 HVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSNGPPCVLCELDE 1140

Query: 1224 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNSSSSKVGHEEP 1283
            LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQ+KNSSSSKVGHEEP
Sbjct: 1141 LFQEYEARLFRLNKVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQDKNSSSSKVGHEEP 1200

Query: 1284 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHLLEYMRKEYGHARS 1343
            NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLG+ESI AANKQLHLLEYMRKEYGHARS
Sbjct: 1201 NKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGRESIAAANKQLHLLEYMRKEYGHARS 1260

Query: 1344 LAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQYSSDKFMSLAMLS 1403
            LAIAQA VLNAHDEIKMATTRLCLRDNDDDSSA++ISEHELPAASVQYSSDKFMSLAMLS
Sbjct: 1261 LAIAQAHVLNAHDEIKMATTRLCLRDNDDDSSAFSISEHELPAASVQYSSDKFMSLAMLS 1320

Query: 1404 RVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQE 1463
            RVKGKLRYLKGLVQSKQTIPL SSSDLALTQEPAITSTAMEQKNENTLKADEESCP+CQE
Sbjct: 1321 RVKGKLRYLKGLVQSKQTIPLHSSSDLALTQEPAITSTAMEQKNENTLKADEESCPICQE 1380

Query: 1464 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1523
            KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN
Sbjct: 1381 KLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKN 1440

Query: 1524 ETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1583
            ETLDPSTLLETSR+HEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV
Sbjct: 1441 ETLDPSTLLETSRKHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDV 1500

Query: 1584 LQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLI 1643
            L YAFAANNI+FIRMKGGRKSQTAISEFRGQKINAKENKKKRLST+LPELRSAQVLLLLI
Sbjct: 1501 LHYAFAANNISFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTDLPELRSAQVLLLLI 1560

Query: 1644 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1703
            QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK
Sbjct: 1561 QHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYK 1620

Query: 1704 LNRSRESSSFITGNAKNQDQPCLTLKEVESLFASKALPLGENDDKGSETLRQLAPSMAAT 1763
            LNRSRESSSFITGNAKNQDQP LTLKEVESLFASKA PLGENDDKGSETLRQLAPSMAAT
Sbjct: 1621 LNRSRESSSFITGNAKNQDQPFLTLKEVESLFASKASPLGENDDKGSETLRQLAPSMAAT 1624

Query: 1764 IAAERRLREHT 1774
            IAAERRLREHT
Sbjct: 1681 IAAERRLREHT 1624

BLAST of PI0009324 vs. TAIR 10
Match: AT2G40770.1 (zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding )

HSP 1 Score: 1778.8 bits (4606), Expect = 0.0e+00
Identity = 964/1707 (56.47%), Postives = 1214/1707 (71.12%), Query Frame = 0

Query: 84   MGRRKQKQPHRSGGIRFEDRGDPKTQLDINGVVETAAESSDIKLDEVNEPIFVEVDRSGW 143
            MGRRKQ +P R+ G+     G    +  + G  + A  S D  ++++++P +V +  S  
Sbjct: 1    MGRRKQSKPQRAVGL-ITPTGSESDRNQLPG--DEAEGSGDKNVEDIDKPYYVNICLSSR 60

Query: 144  YS--TEHHDISEVFLTDVRLEHPFVS---FCLDKSVCENSRYSLRFRLCNVNGSLVDRIK 203
             S   ++ D++EV LT+  +     S   F     V  +   SLRFRLCNV  + VDRIK
Sbjct: 61   VSEQQQNFDLAEVVLTNFSVRERVGSSSTFTKPIEVDHDLDCSLRFRLCNVT-NFVDRIK 120

Query: 204  FGHWPVLSSNDIFLELIERDMEEDVKACSVVLSGNFDGPDKAISGLVHLANLKLMTLRPV 263
             GHWPVLSS+DI LEL++  + +D +  SV+ S +FDGP + +SGL HLA++K +TLR +
Sbjct: 121  LGHWPVLSSSDITLELVDNKVSDD-EVGSVIWSASFDGPGEGVSGLAHLASIKFLTLRLM 180

Query: 264  DGISFSQNTGSFRLRVEILSSAFDACESIFDNGRQLWKKSMMNTIAWLRPEVVLSEVKYG 323
             G   ++   S R+RVE+L  AFDAC+S+ +N RQ+WKKSM++ ++WLRPEV+ SE +YG
Sbjct: 181  PG---NEGLLSPRVRVEMLQQAFDACDSLLENTRQIWKKSMIHVMSWLRPEVMTSEARYG 240

Query: 324  VVKSTNTDTHLHHEVGDDTSNSRKRVNFDTTGFYDAIKPSKDEPMLDEDIPNLLPKLRPY 383
              +    D        D+T +S K+ +FD   FY+AIKPSK + ML +DI +LLP+LRPY
Sbjct: 241  -TRFNVKDIESSVVTEDETLDSSKQSSFDAAAFYEAIKPSKTDAMLGDDIADLLPELRPY 300

Query: 384  QRRAAYWMVQREKGFSGNSGLGENVQLISPLCLALKCLDTCLRVFYNPFSGNLTLNAEQI 443
            QRRAAYWMVQRE+G     G  E+ Q ISPL +++  LD+  ++F NPFSGN++L  E  
Sbjct: 301  QRRAAYWMVQRERGDPITLGDKEDNQFISPLSISVGFLDSATKMFLNPFSGNISLTPEYF 360

Query: 444  LPHVYGGILADEMGLGKTIELLACILSH------QMSVFEGGKGFDVEVQQLVKDQRTEF 503
             P + GGILADEMGLGKT+ELLACI SH      ++SV  G    DV             
Sbjct: 361  SPRIQGGILADEMGLGKTVELLACIFSHRKPAEDEISVSNGSSVTDV--------LNAGL 420

Query: 504  KRLKRERVECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNSR 563
            +RLKRERVEC+CGAVSE+++YKG+WVQCD+CDAWQHADCVGYSPKG+  K     D  + 
Sbjct: 421  RRLKRERVECICGAVSESHKYKGVWVQCDLCDAWQHADCVGYSPKGKGKKDSQHIDEKAS 480

Query: 564  EKRNNRSTLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTR 623
            +K++ +    + VR  E++C  C EL+Q T SP++TGATLIVCPAPIL QW +EI RHTR
Sbjct: 481  QKKSKKDATEIIVREGEYICQMCSELLQVTASPISTGATLIVCPAPILPQWHSEITRHTR 540

Query: 624  PGSIKLLVYEGVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMR 683
             GS+   +YEGVR+ SL     + I EL+N+DIVLT+YDVLKEDL+HD DRH+GDR  +R
Sbjct: 541  LGSLITCIYEGVRNASLSEEPMIDITELLNADIVLTTYDVLKEDLTHDFDRHDGDRHCLR 600

Query: 684  FQKRYPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLE 743
            FQKRYPV+PTPLTRIFWWRICLDE QMVESNA AATEMA RLY  H WCITGTPIQRKL+
Sbjct: 601  FQKRYPVIPTPLTRIFWWRICLDEAQMVESNAAAATEMALRLYTKHRWCITGTPIQRKLD 660

Query: 744  DLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDELQ 803
            DL+GLL+F+KA+PF+V RWW+EVIRDPYERRD  AMEFTHKFFKQ+MWRS K+HV DELQ
Sbjct: 661  DLFGLLKFLKANPFDVSRWWIEVIRDPYERRDTKAMEFTHKFFKQVMWRSSKVHVADELQ 720

Query: 804  LPPQEEQVTWLKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVPSDL 863
            LPPQEE V+WLKFS IEEHFY RQH++CVSYAREVI+ LK D +KR      SSD P   
Sbjct: 721  LPPQEECVSWLKFSAIEEHFYSRQHDTCVSYAREVIETLKRDILKR---GHTSSDNP--- 780

Query: 864  LITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVSKTRIEGEEAL 923
            L+THA+A KLL++LLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLV KT+ EGEEAL
Sbjct: 781  LVTHAEAAKLLNSLLKLRQACCHPQVGSSGLRSLQQSPMTMEEILMVLVKKTQSEGEEAL 840

Query: 924  RRSVVALNALAGIAIIEKKFSEAFSLYKEALELAEENNEDFRLDPLLSIHIHHNLAEILP 983
            R  +VALN +A IA+++++FSEA SLYKEAL + EE+ EDFRLDPLL+IHI HNLAEILP
Sbjct: 841  RVLIVALNGIAAIAMLKQEFSEAVSLYKEALSITEEHAEDFRLDPLLNIHILHNLAEILP 900

Query: 984  LAVNQSQSPLKDQFYPRTCEVKASRMDDSEKYDDHVHIMKKQKVSETLYAPCSEDNTGKM 1043
            +A +     L     P T      ++D   K DDH    K+Q+++E      + D++  +
Sbjct: 901  MAKSYG-GKLSASGRPET------KID--VKDDDHHRASKRQRINE--LESLTHDSSETV 960

Query: 1044 IDHPLQLTGKDTNAKKEENYEPHRSSSYFDEISVRKVCEVMRQKYLAVFSSKLSIAQQEF 1103
              H  +    D   KK+   E H      D      VC+ ++ KYL+ F+SKLS AQ EF
Sbjct: 961  --HQREAIAPDNGLKKDG--ECHEECKTLD-----IVCDTLKVKYLSAFNSKLSAAQHEF 1020

Query: 1104 TKSYMQVRSELKDRENLNDVWWLEAVHHAEQNKDFSHELIRKIEEAVSGNLNN-SKSRVG 1163
             KSY QV   L +      VWWL+A+   EQNKDFS EL RKIEEA+ GNLNN S SR  
Sbjct: 1021 KKSYNQVSESLSNMGKQRSVWWLDALQLTEQNKDFSSELTRKIEEALHGNLNNSSSSRES 1080

Query: 1164 SRFRSISALKYHVQSGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCRNCQADSN 1223
            SRFR+I  +K H+Q+ +D+LE SRK V+DR+LEIDQTME PK EDIER+  C+ C  +S+
Sbjct: 1081 SRFRTIHGMKLHLQTCMDMLERSRKKVIDRILEIDQTMEKPKLEDIERISNCKYCNKNSD 1140

Query: 1224 GPPCVLCELDELFQEYEARLFRLNKVQGGM--VTSVEEAVEAQKKKSALNRFYWSLLQQN 1283
            GPPC+ CELDELFQEYEARLFRLNK + G+  + + EE V  QKK+ A N F + L  ++
Sbjct: 1141 GPPCIHCELDELFQEYEARLFRLNKSRRGVMEIAAAEETVHLQKKRDARNLFLFGLSSRS 1200

Query: 1284 KNSSSSKVGHEEPNKRDVGEKVMVSKHPSELEVVLGVIKNFCKTQLGKESILAANKQLHL 1343
            K+ ++S+   EEP KR+ G+ V++SK PSE E+VLGVI+N CKT L +ES LAA K LH 
Sbjct: 1201 KDLNASRGDDEEPTKRNAGDIVVLSKSPSETEIVLGVIRNHCKTHLDRESKLAATKHLHT 1260

Query: 1344 LEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLRDNDDDSSAYAISEHELPAASVQ 1403
            LE MRKEY HAR LA  QAQ+L A+DEI M+T RL LR+++DD+S YA+   EL  ASV 
Sbjct: 1261 LEVMRKEYVHARVLARDQAQLLRAYDEINMSTMRLQLRESEDDTSIYALGRDELDVASVL 1320

Query: 1404 YSSDKFMSLAMLSRVKGKLRYLKGLVQSKQTIPLDSSSDLALTQEPAITSTAMEQKNENT 1463
             ++DKFM+ + L  +KGKLRYLKGL++SKQ    +S    +   E    S   EQ++EN 
Sbjct: 1321 NTNDKFMAQSSLLSIKGKLRYLKGLMKSKQKQESESPDLSSPIHETVDASDPAEQESENL 1380

Query: 1464 LKADEESCPVCQEKLSNQKMVFQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHT 1523
            LK D E+CP+C E L NQKMVFQCGH TCC C FAMTE+      +Q KWVMCP CRQHT
Sbjct: 1381 LKRD-EACPICHEILRNQKMVFQCGHSTCCNCFFAMTERKSVQETLQ-KWVMCPICRQHT 1440

Query: 1524 DFGNIAYADDSKNETLDPSTLLETSREHEMSITVQGSYGTKIEAVVRRILWIKYTDSEAK 1583
            D  NIAYADD +N     S+  +  ++ E S+ VQGSYGTKIEAV RRILWIK +D + K
Sbjct: 1441 DVRNIAYADDRRNS----SSSDQDHKDSEASLVVQGSYGTKIEAVTRRILWIKSSDPQTK 1500

Query: 1584 VLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNL 1643
            VLVFSSWNDVLDVL++AFAAN+IT IRMKGGRKSQTAIS+F+G      E + ++ +++ 
Sbjct: 1501 VLVFSSWNDVLDVLEHAFAANSITCIRMKGGRKSQTAISKFKG-----SEKETQKTNSHQ 1560

Query: 1644 PELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHR 1703
             E +S QVLLLL+QHGANGLNLLEAQHV+LVEPLLNPAAEAQA+ RVHRIGQE  T VHR
Sbjct: 1561 KEEKSIQVLLLLVQHGANGLNLLEAQHVILVEPLLNPAAEAQAVGRVHRIGQEKPTLVHR 1620

Query: 1704 FIVKDTVEESIYKLNRSRES--SSFITGNAKNQDQPCLTLKEVESLFAS---KALPLGEN 1763
            F+V  TVEESIYKLNR++ +  SSF + N KNQDQ  LTLK++ESLFAS   +   + +N
Sbjct: 1621 FLVSGTVEESIYKLNRNKNTNLSSFSSRNTKNQDQQFLTLKDLESLFASPTAETAEMEQN 1653

Query: 1764 DDKGSETLRQLAPSMAATIAAERRLRE 1772
              +  E LR L PS+AA +AAERR++E
Sbjct: 1681 PGERQENLRDLPPSVAAALAAERRMKE 1653

BLAST of PI0009324 vs. TAIR 10
Match: AT5G22750.1 (DNA/RNA helicase protein )

HSP 1 Score: 179.1 bits (453), Expect = 3.1e-44
Identity = 142/517 (27.47%), Postives = 211/517 (40.81%), Query Frame = 0

Query: 371 LLPKLRPYQRRAAYWMVQREKGFSGNSGLGENVQLISPLCLALKCL--DTCLRVFYNPFS 430
           LL +LRPYQ++A +WM Q EKG    +   E   ++ P C    CL     L V+ N F+
Sbjct: 344 LLCELRPYQKQALHWMTQLEKG----NCTDEAATMLHP-CWEAYCLADKRELVVYLNSFT 403

Query: 431 GNLTLNAEQILPHVYGGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQ 490
           G+ T++    L    GGILAD MGLGKT+  ++ +L+H         GF           
Sbjct: 404 GDATIHFPSTLQMARGGILADAMGLGKTVMTISLLLAHSWKA--ASTGF----------- 463

Query: 491 RTEFKRLKRERVECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTED 550
                                            +C  ++    +  S       P+    
Sbjct: 464 ---------------------------------LCPNYEGDKVISSSVDDLTSPPVKATK 523

Query: 551 GNSREKRNNRSTLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEIL 610
               +KR                       +    S +  G  LIVCP  +L QW+ EI 
Sbjct: 524 FLGFDKR-----------------------LLEQKSVLQNGGNLIVCPMTLLGQWKTEIE 583

Query: 611 RHTRPGSIKLLVYEG---VRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHD-SDRH 670
            H +PGS+ + V+ G    +D  LL            SD+V+T+Y VL  + S + S  H
Sbjct: 584 MHAKPGSLSVYVHYGQSRPKDAKLLS----------QSDVVITTYGVLTSEFSQENSADH 643

Query: 671 EGDRRFMRFQKRYPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITG 730
           EG                 +  + W+RI LDE   ++++ +  +  A+ L A   WC+TG
Sbjct: 644 EG-----------------IYAVRWFRIVLDEAHTIKNSKSQISLAAAALVADRRWCLTG 703

Query: 731 TPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLK 790
           TPIQ  LEDLY LLRF++  P+    WW ++++ P+E  D   ++      K IM R  K
Sbjct: 704 TPIQNNLEDLYSLLRFLRIEPWGTWAWWNKLVQKPFEEGDERGLKLVQSILKPIMLRRTK 736

Query: 791 IHVTDE----LQLPPQEEQVTWLKFSPIEEHFYQRQHE-SCVSYAREVIQGLKDDFVKRK 850
                E    L LPP + +V + + S  E  FY    + S V + + V QG         
Sbjct: 764 SSTDREGRPILVLPPADARVIYCELSESERDFYDALFKRSKVKFDQFVEQG--------- 736

Query: 851 VPDCVSSDVPSDLLITHADAGKLLSTLLKLRQACCHP 877
                         + H +   +L  LL+LRQ C HP
Sbjct: 824 -------------KVLH-NYASILELLLRLRQCCDHP 736

HSP 2 Score: 107.8 bits (268), Expect = 8.8e-23
Identity = 80/311 (25.72%), Postives = 143/311 (45.98%), Query Frame = 0

Query: 1426 SSDLALTQEPAITSTAMEQKNENTLKADEESCPVCQEKLSNQKMVFQCGHITCCKCLFAM 1485
            SS L    +   +   +++  E   K ++  CP+C E L +  ++  C H  C +CL A 
Sbjct: 763  SSGLEREGKDVPSEAFVQEVVEELRKGEQGECPICLEALED-AVLTPCAHRLCRECLLAS 822

Query: 1486 TEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTLLETSREHEMSITVQG 1545
               +  G        +CP CR                 T+    L+    E    + V+ 
Sbjct: 823  WRNSTSG--------LCPVCR----------------NTVSKQELITAPTESRFQVDVEK 882

Query: 1546 SY--GTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKS 1605
            ++   +KI A++  +  ++   S +K ++FS W   LD+LQ   + NN +F+R+ G    
Sbjct: 883  NWVESSKITALLEELEGLR--SSGSKSILFSQWTAFLDLLQIPLSRNNFSFVRLDG---- 942

Query: 1606 QTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPL 1665
                           + +++++     E  S  VLL+ ++ G  G+NL  A +  +++P 
Sbjct: 943  ------------TLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPW 1002

Query: 1666 LNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYKLNRSRESSSFITGNAKNQDQP 1725
             NPA E QA+ R+HRIGQ  +  + RFIVK TVEE +  +   ++    I+G   +Q+  
Sbjct: 1003 WNPAVEEQAVMRIHRIGQTKEVKIRRFIVKGTVEERMEAVQARKQ--RMISGALTDQEVR 1028

Query: 1726 CLTLKEVESLF 1735
               ++E++ LF
Sbjct: 1063 SARIEELKMLF 1028

BLAST of PI0009324 vs. TAIR 10
Match: AT5G43530.1 (Helicase protein with RING/U-box domain )

HSP 1 Score: 166.4 bits (420), Expect = 2.1e-40
Identity = 141/522 (27.01%), Postives = 208/522 (39.85%), Query Frame = 0

Query: 375  LRPYQRRAAYWMVQREKGFSGNSGLGENVQLISPLCLALK-CLDTCLRVFYNPFSGNLTL 434
            LRPYQ++A YWM + EKG      + +  + + P   A + C +    ++ N FSG  T+
Sbjct: 616  LRPYQKQALYWMSESEKGID----VEKAAETLHPCWEAYRICDERAPSIYLNIFSGEATI 675

Query: 435  NAEQILPHVYGGILADEMGLGKTIELLACILSHQMSVFEGGKGFDVEVQQLVKDQRTEFK 494
                      GGILAD MGLGKT+  +A IL+        G+G       LV D      
Sbjct: 676  QFPTATQMARGGILADAMGLGKTVMTIALILARP------GRGNPENEDVLVADV----- 735

Query: 495  RLKRERVECLCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNSRE 554
                                                                     + +
Sbjct: 736  ---------------------------------------------------------NAD 795

Query: 555  KRNNRSTLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEILRHTRP 614
            KRN +                  E+  A  +  A G TLI+CP  +L QW+ E+  H++P
Sbjct: 796  KRNRK------------------EIHMALTTVKAKGGTLIICPMALLSQWKDELETHSKP 855

Query: 615  GSIKLLVYEGVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDRHEGDRRFMRF 674
             ++ +LVY G       G  +     + + D+VLT+Y VL      D             
Sbjct: 856  DTVSVLVYYG-------GDRTHDAKAIASHDVVLTTYGVLTSAYKQD------------- 915

Query: 675  QKRYPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCITGTPIQRKLED 734
                 +  +   RI W+RI LDE   ++S  T A +    L +   WC+TGTP+Q KLED
Sbjct: 916  -----MANSIFHRIDWYRIVLDEAHTIKSWKTQAAKATFELSSHCRWCLTGTPLQNKLED 975

Query: 735  LYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSLKIHVTDE--- 794
            LY LL F+   P+    WW ++I+ PYE  DP  ++      + +M R  K     E   
Sbjct: 976  LYSLLCFLHVEPWCNWAWWSKLIQKPYENGDPRGLKLIKAILRPLMLRRTKETRDKEGSL 1000

Query: 795  -LQLPPQEEQVTWLKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKVPDCVSSDVP 854
             L+LPP + QV   + S  E  FY         + R  +Q   D FV +           
Sbjct: 1036 ILELPPTDVQVIECEQSEAERDFY------TALFKRSKVQ--FDQFVAQ----------- 1000

Query: 855  SDLLITHADAGKLLSTLLKLRQACCHPQVGSSGLRSLQQSPM 892
              +L  +A+   +L  LL+LRQ C HP +  S   S Q + +
Sbjct: 1096 GKVLHNYAN---ILELLLRLRQCCNHPFLVMSRADSQQYADL 1000

HSP 2 Score: 101.7 bits (252), Expect = 6.3e-21
Identity = 88/326 (26.99%), Postives = 143/326 (43.87%), Query Frame = 0

Query: 1417 SKQTIPLDSSSDLALTQEP------AITSTAMEQKNENTLKADEESCPVCQEKLSNQKMV 1476
            S+Q   LDS +   L   P      A +   +E+  ++    + + CP+C E  ++  ++
Sbjct: 994  SQQYADLDSLARRFLDNNPDSVSQNAPSRAYIEEVIQDLRDGNSKECPICLES-ADDPVL 1053

Query: 1477 FQCGHITCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1536
              C H  C +CL         G        +CP CR                  L  + L
Sbjct: 1054 TPCAHRMCRECLLTSWRSPSCG--------LCPICR----------------TILKRTEL 1113

Query: 1537 LETSREHEMSITVQGSY--GTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFA 1596
            +    +    + V  ++   +K+  +++ +  IK + S  K +VFS W   LD+L+    
Sbjct: 1114 ISCPTDSIFRVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIPLR 1173

Query: 1597 ANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTNLPELRSAQVLLLLIQHGANG 1656
                 F+R  G              K+  K  +K     N  E +   +LL+ ++ G  G
Sbjct: 1174 RRGFEFLRFDG--------------KLAQKGREKVLKEFN--ETKQKTILLMSLKAGGVG 1233

Query: 1657 LNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVHRFIVKDTVEESIYKLNRSRE 1716
            LNL  A  V L++P  NPA E QAI R+HRIGQ+   FV RFIVKDTVEE + ++   ++
Sbjct: 1234 LNLTAASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQARKQ 1276

Query: 1717 SSSFITGNAKNQDQPCLTLKEVESLF 1735
                I G   +++     L+E++ LF
Sbjct: 1294 --RMIAGALTDEEVRSARLEELKMLF 1276

BLAST of PI0009324 vs. TAIR 10
Match: AT3G20010.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 122.5 bits (306), Expect = 3.5e-27
Identity = 120/481 (24.95%), Postives = 186/481 (38.67%), Query Frame = 0

Query: 444 GGILADEMGLGKTIELLACILSHQ-MSVFEGGKGFDVEVQQLVKDQRTEFKRLKRERVEC 503
           GGILAD+ GLGKT+  +A IL  + +S  +       E + LV D   E    K E    
Sbjct: 304 GGILADDQGLGKTVSTIALILKQKIVSQLKSESSCKQETEALVLDADDESDNAKHE---- 363

Query: 504 LCGAVSENYRYKGLWVQCDICDAWQHADCVGYSPKGRIVKPIDTEDGNSREKRNNRSTLN 563
                       G  V+ ++     +++    S  G      D  D +  EK  +    +
Sbjct: 364 -----------SGSHVKPEL-KVSSNSETSVLSACGN-----DENDSSDMEKAEDEEANS 423

Query: 564 VTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQAEI-LRHTRPGSIKLLVY 623
            T               +A         TLIVCPA ++ QW  E+  + +    + +LVY
Sbjct: 424 ST---------------RAFQWKRPAAGTLIVCPASVVRQWARELDEKVSEESKLSVLVY 483

Query: 624 EGVRDTSLLGTLSVQINELINSDIVLTSYDVL------------KEDLSHDSDRHEGDRR 683
            G   T          NEL   D+V+T+Y ++             E+   ++DR+     
Sbjct: 484 HGSNRTK-------DPNELAEYDVVVTTYAIVTNEAPNKFLVDEDENDEKNTDRYGLASG 543

Query: 684 FMRFQKRYPVLPT----------------------PLTRIFWWRICLDEGQMVESNATAA 743
           F   +KR  V+                        PL ++ W+RI LDE Q +++  T  
Sbjct: 544 FSNNKKRKVVVGASKKSKRRGRKSTNDTSSEPDCGPLGKVGWFRIVLDEAQTIKNYRTQM 603

Query: 744 TEMASRLYASHHWCITGTPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGA 803
                 L A   WC++GTPIQ  ++DLY   RF++  P+ V++ +   I+ P  R     
Sbjct: 604 ARSCCTLRAKRRWCLSGTPIQNTIDDLYSYFRFLRYDPYAVYKSFYSTIKVPISRNSCQG 663

Query: 804 MEFTHKFFKQIMWRSLKIHVTDE---LQLPPQEEQVTWLKFSPIEEHFYQRQHESCVSYA 863
            +      + IM R  K  + D    + LPP+   ++ + FS  E  FY++      S  
Sbjct: 664 YKKLQAVLRAIMLRRTKGTLLDGKPIINLPPKVVNLSQVDFSVAERSFYKKLEADSRSQF 712

Query: 864 REVIQGLKDDFVKRKVPDCVSSDVPSDLLITHADAGKL-------LSTLLKLRQACCHPQ 879
           +                              +ADAG L       L  LL+LRQAC HPQ
Sbjct: 724 K-----------------------------AYADAGTLSQNYANILLLLLRLRQACDHPQ 712

HSP 2 Score: 71.2 bits (173), Expect = 9.2e-12
Identity = 75/333 (22.52%), Postives = 134/333 (40.24%), Query Frame = 0

Query: 1419 QTIPLDSSSDLALTQEPAITSTAMEQKNE--NTLKADEESCPVCQEKLSNQKMVFQCGHI 1478
            Q +   +S  +    E A+     E ++   N L++    C  C E    + +V  CGHI
Sbjct: 711  QLVKRYNSDPVGKVSEAAVRRLPREARSRLINRLESSSAICYECNEP-PEKPVVTLCGHI 770

Query: 1479 TCCKCLFAMTEKTLHGSKIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTLLETSRE 1538
             C +C+        +   +       P C+Q     ++ +++ S        +   +S +
Sbjct: 771  FCYECVLEYITGDENTCPV-------PRCKQQL-ARDVVFSESSLRNCTSDDSGCSSSHD 830

Query: 1539 HEMSITV---QGSYGTKIEAVVRRILWIKYTDS--------------------------- 1598
            + +  +V   +    +KI+AV+  +  +   DS                           
Sbjct: 831  NGLDRSVFQKRDFCSSKIKAVLDILQSLSQPDSPNSAQHGQMPSSSRPYDDDDVTIVEPM 890

Query: 1599 ----------EAKVLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKSQTAISEFRGQKIN 1658
                        K ++FS W  +LD+++     + I F R+ G               + 
Sbjct: 891  RLHSSSPSQGAVKTIIFSQWTGMLDLVELRILESGIEFRRLDG------------TMSLA 950

Query: 1659 AKENKKKRLSTNLPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRV 1710
            A++   K  S   P+++   V+L+ ++ G  GLN++ A HV+L++   NP  E QAI R 
Sbjct: 951  ARDRAVKEFSKK-PDVK---VMLMSLKAGNLGLNMVAACHVILLDLWWNPTTEDQAIDRA 1010

BLAST of PI0009324 vs. TAIR 10
Match: AT1G11100.1 (SNF2 domain-containing protein / helicase domain-containing protein / zinc finger protein-related )

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-26
Identity = 91/341 (26.69%), Postives = 153/341 (44.87%), Query Frame = 0

Query: 545 DTEDGNSREKRNNRSTLNVTVRAEEHVCTPCLELMQATDSPMATGATLIVCPAPILFQWQ 604
           D+++G   +  +++   N     E+ +C       +    P A   TLIVCP  ++ QW 
Sbjct: 598 DSKNGGCNQSDHSQVVFNENKVVEDSLC-------KMRGRPAA--GTLIVCPTSLMRQWA 657

Query: 605 AEILRH-TRPGSIKLLVYEGVRDTSLLGTLSVQINELINSDIVLTSYDVLKEDLSHDSDR 664
            E+ +  T    + +LVY G   T          +EL   D+V+T+Y ++ +    D + 
Sbjct: 658 DELRKKVTLEAHLSVLVYHGCSRTK-------DPHELAKYDVVITTYSLVSKRKHMDCEP 717

Query: 665 HEGDRRFMRFQKRYPVLPTPLTRIFWWRICLDEGQMVESNATAATEMASRLYASHHWCIT 724
            E              L  PL ++ W+R+ LDE Q +++  T A+   S L+A   WC++
Sbjct: 718 VE-------------FLSGPLAQVSWFRVVLDEAQSIKNYKTQASIACSGLHAKRRWCLS 777

Query: 725 GTPIQRKLEDLYGLLRFVKASPFNVHRWWVEVIRDPYERRDPGAMEFTHKFFKQIMWRSL 784
           GTPIQ  + DLY   RF+K  P++ ++ + E I++P         +      K++M R  
Sbjct: 778 GTPIQNSIADLYSYFRFLKYDPYSSYQTFCETIKNPISSYPGEGYKTLQAILKKVMLRRT 837

Query: 785 KIHVTD---ELQLPPQEEQVTWLKFSPIEEHFYQRQHESCVSYAREVIQGLKDDFVKRKV 844
           K  + D    + LPP+  ++  + F+  E  FY +    C S         +D F +   
Sbjct: 838 KDTLLDGKPVISLPPKSIELRRVDFTKEERDFYSKL--ECDS---------RDQFKEYAE 887

Query: 845 PDCVSSDVPSDLLITHADAGKLLSTLLKLRQACCHPQVGSS 882
              V  +  + LL+           LL+LRQAC HP + SS
Sbjct: 898 AGTVKQNYVNILLM-----------LLRLRQACGHPLLVSS 887

HSP 2 Score: 69.3 bits (168), Expect = 3.5e-11
Identity = 45/139 (32.37%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 1569 KVLVFSSWNDVLDVLQYAFAANNITFIRMKGGRKSQTAISEFRGQKINAKENKKKRLSTN 1628
            K +VF+ W  +LD+L+    ++ I + R  G  K      +   Q  N            
Sbjct: 1073 KAIVFTQWTKMLDLLEAGLKSSGIQYRRFDG--KMTVPARDAAVQDFN-----------T 1132

Query: 1629 LPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHRIGQENKTFVH 1688
            LP++    V+++ ++  + GLN++ A HV++++   NP  E QAI R HRIGQ     V 
Sbjct: 1133 LPDV---SVMIMSLKAASLGLNMVAACHVIMLDLWWNPTTEDQAIDRAHRIGQTRPVKVV 1192

Query: 1689 RFIVKDTVEESIYKLNRSR 1708
            RF VKDTVE+ I  L + +
Sbjct: 1193 RFTVKDTVEDRILALQQKK 1195