PI0009288 (gene) Melon (PI 482460) v1

Overview
NamePI0009288
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr08: 7059454 .. 7062390 (+)
RNA-Seq ExpressionPI0009288
SyntenyPI0009288
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACAAAAGGTCACGTCGCTCCTCTCTCGACCTCGTAGCCCAAAGCCTGGGTTAACCCATCCGCTCTGTCTTCTTCTTTCTTCTCTCTCTTCTCTCTCTTCTCTTCGTTTGTTCAGTTCAAGAAAAAGGAAATTGGGTTGATCCACTTTTCTCTTTCGTCTTCCTAAAGGTGAATTTCTGCTGCTACCCATTTTCTAGGGTTCCTTTTTCCTCCACCTTTTTCTGCAATTGAATGCCTCTCTAACAGTTTTACCCTCTTTTTCTGTCTCTTTGCTCGTTTTCAGTTTGTGGGTCTTTGTTGTTTTCAATCTTTGTTGCTTTGAGGTTTTTGGGGAATTTGTTGAAATGGGTGGGTACTGTTTGGATCTTTGTTGGTGGGTGTCTTTCATTGGTTTCTGTGGCTTATTTGGAATGAAAATATTGAGTTGGGTTGTGCTTTTACGTTGTGTGTAGTGGGAATTTTTGAAGTTGTGTTCTTGTATGCTTTGTTTGGACTATGGAATATAGTTTCGATTTTTTTCGTTTCTTGCTCTGTATTTGGTGAAATCGGTCTCAACTTGGGCAGAAATTAATGCCCTCAACTAAGTTAAGCTCTCATTTTTCTATTTCTTTTAAGAATTTGGCTAATTATATGGTGATTTCTGTGGCTGGAGTAGAATGTTTTTGTGACTGTTGGTGGGATTTGGACATTTATATGTTTAACTTTTTTGGTGTTCTGTTGGATGAATATGGTATGATTGATTTATTTCAATCTTTACTCAATTGTGAGTAATTTTTTAATATAGTTTTTAGAAGGTATTAGTTGTACCACTTTTTTCGATAGTGTACAATTTCTCGACTTTAGTATAGTCCAAGTGGTTAAGACATATGCTCTCAACCAAGATGGCTAAAGATTGATTCGAATCACCAACCCACTTGTTTTTTTTTTTACTAAGAATTAAACAAAAACAAACGAACAAAATTGAATCATTTTATGTTCTATTGATCTTCATTTTTATGTGAACAGAGTTAAATTCTAAGCACTGAGATATTTCATGAGATTTTGGGTACTTTTTGTCTGAAGAGTATGCCCCCATCTTTACATAGTTAAACAAGGATAGTTAGACTGAAGATGAATAGGCCTAGCAGAGCATGTATGCCTGCTAGCAGCTGTGTAATTTCATCGGAGCGGAGTCCTTTTGTAAATGTTTTATTCAATTCCTTGAGATTTAGCTCAATCTTCTTTTCTTTTTTTTAAAGGCTTTTTAAGTACAAACAGGGTGGGGATTTGAACCTGAGACCACTTAGACCTCAGATACATACAGGTGCTAGTTGAGCTAAACTCTTGATGGCTAGCTCAATCTTCTAATGTTTCATTGAAATCTAAAAAATAATATGGAAGTATGGATATCATATGGGGTTATGTTTGTGCAAGTATGTATTTGTTGAAAATCCAAAAAACGGATTACAAATGGTAGTCCAGAGTGTGGTAGATGAGAAGTATTAAGTGTTTGATCTGTGGGTTTTCTGATTGTTATATATGCATAAACGATAAATTTTGTTGATGGTTGGATCTGAGTGGTATGTATGGATTCTAAAATGATGGTTTGTTTCATTTCAGCTTGGTTTATAATATATGGTTTGGTTCTTTAATTCTTTTATGTCACATCGGTTGCTTTTTATTTAATCCACCCGTTGAAGTTGATTTAGTGAATATATATTGTGAAAGTAAGACATTATGCTGAGATAACATATTTTGGTGCTTGACCAGGGATAAGTTTTGGTGTTATTCCGTTTATATATTTTAGTAGAGACCAGGATAAGAGAGGATGTCAGCTGGTCGGAACACCCATTGGTGTCATAGATGCAGAAGGTCGGTACATCTCCGTGGTAGAGATGCAGTTTGTCCCAACTGCAATGAAGGATTTATCCAAGAACTTGATGTGGTGCCTGGAAATCCATTTGATTTCTTTGGTTTAGATCATGATGAAGATCGCGATCATGGTCTGGGTGCTTTCTCTGCTTTTATGAGACAACGTTTAGCCGAAAGAAATGACATGAGAGGAAGATCAGAGTCACTTTTTGAGCGTAGCCCAGGATTCGGTCCATTATTAATATTTGGTGGTCAAATACCGTTGCGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCTCCCGGCATTGGTATTACACGGGGTGATAGTGGTGACTACTTCATAGGCCCCGGATTGGAGGAGCTATTCGAACAGCTTTCAGCAAATGGTCATCGCGGCCCTCCACCAGCATCTAGATCATCAATCGACGCAATGCCAACAGTTAAGATCACGCAACGACATCTTCGCTCAGATTCACATTGTCCAGTTTGTAAAGAGAAGTTTGAACTGGGGTCTGAAGCAAGGCAGATGGCCTGTAATCACATGTATCACTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAATTCTTGCCCCGTCTGCCGCCAGGAACTGCCACCACAGGGAATAGGTGGTGGGGGCGGGGGACATAGCACCAACGATCAAAACCGAAGCAACAGCTACAACAATGGGAGTGGCTCCCGGGTCAATCCAGGTAGACGCAATCCTTTCTCTTATCTGTGGCCATTTCGGTCATCTAGTTCTAACTCCAACCATGGCGGAGCAGGGAGCAGTGAACCGTCGAACCACCAAATGGGGTATTCAGGATATCGATACACTAATTCACCATGAATTGAAGTTTGGAGATTGTTTGTTTGTAGTACAAAGGGTTGAAGATCAGCTTTGTTTGAAGAAGATACACAGGCTGTTGTATTTTTTGTGCAAATTTTCCTATGTAAATGACCTTATAATTAATTTGTAGCTGCTTATTGCTTCTGCTGATGTTTTCTTCATCTTCCATGTCAAAAAGATGACACATCAATTATTAGTATAAAATGGGAAAAATATTTTACTGCCTCT

mRNA sequence

ATGAAAACAAAAGGTCACGTCGCTCCTCTCTCGACCTCGTAGCCCAAAGCCTGGGTTAACCCATCCGCTCTGTCTTCTTCTTTCTTCTCTCTCTTCTCTCTCTTCTCTTCGTTTGTTCAGTTCAAGAAAAAGGAAATTGGGTTGATCCACTTTTCTCTTTCGTCTTCCTAAAGGGATAAGTTTTGGTGTTATTCCGTTTATATATTTTAGTAGAGACCAGGATAAGAGAGGATGTCAGCTGGTCGGAACACCCATTGGTGTCATAGATGCAGAAGGTCGGTACATCTCCGTGGTAGAGATGCAGTTTGTCCCAACTGCAATGAAGGATTTATCCAAGAACTTGATGTGGTGCCTGGAAATCCATTTGATTTCTTTGGTTTAGATCATGATGAAGATCGCGATCATGGTCTGGGTGCTTTCTCTGCTTTTATGAGACAACGTTTAGCCGAAAGAAATGACATGAGAGGAAGATCAGAGTCACTTTTTGAGCGTAGCCCAGGATTCGGTCCATTATTAATATTTGGTGGTCAAATACCGTTGCGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCTCCCGGCATTGGTATTACACGGGGTGATAGTGGTGACTACTTCATAGGCCCCGGATTGGAGGAGCTATTCGAACAGCTTTCAGCAAATGGTCATCGCGGCCCTCCACCAGCATCTAGATCATCAATCGACGCAATGCCAACAGTTAAGATCACGCAACGACATCTTCGCTCAGATTCACATTGTCCAGTTTGTAAAGAGAAGTTTGAACTGGGGTCTGAAGCAAGGCAGATGGCCTGTAATCACATGTATCACTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAATTCTTGCCCCGTCTGCCGCCAGGAACTGCCACCACAGGGAATAGGTGGTGGGGGCGGGGGACATAGCACCAACGATCAAAACCGAAGCAACAGCTACAACAATGGGAGTGGCTCCCGGGTCAATCCAGGTAGACGCAATCCTTTCTCTTATCTGTGGCCATTTCGGTCATCTAGTTCTAACTCCAACCATGGCGGAGCAGGGAGCAGTGAACCGTCGAACCACCAAATGGGGTATTCAGGATATCGATACACTAATTCACCATGAATTGAAGTTTGGAGATTGTTTGTTTGTAGTACAAAGGGTTGAAGATCAGCTTTGTTTGAAGAAGATACACAGGCTGTTGTATTTTTTGTGCAAATTTTCCTATGTAAATGACCTTATAATTAATTTGTAGCTGCTTATTGCTTCTGCTGATGTTTTCTTCATCTTCCATGTCAAAAAGATGACACATCAATTATTAGTATAAAATGGGAAAAATATTTTACTGCCTCT

Coding sequence (CDS)

ATGTCAGCTGGTCGGAACACCCATTGGTGTCATAGATGCAGAAGGTCGGTACATCTCCGTGGTAGAGATGCAGTTTGTCCCAACTGCAATGAAGGATTTATCCAAGAACTTGATGTGGTGCCTGGAAATCCATTTGATTTCTTTGGTTTAGATCATGATGAAGATCGCGATCATGGTCTGGGTGCTTTCTCTGCTTTTATGAGACAACGTTTAGCCGAAAGAAATGACATGAGAGGAAGATCAGAGTCACTTTTTGAGCGTAGCCCAGGATTCGGTCCATTATTAATATTTGGTGGTCAAATACCGTTGCGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCTCCCGGCATTGGTATTACACGGGGTGATAGTGGTGACTACTTCATAGGCCCCGGATTGGAGGAGCTATTCGAACAGCTTTCAGCAAATGGTCATCGCGGCCCTCCACCAGCATCTAGATCATCAATCGACGCAATGCCAACAGTTAAGATCACGCAACGACATCTTCGCTCAGATTCACATTGTCCAGTTTGTAAAGAGAAGTTTGAACTGGGGTCTGAAGCAAGGCAGATGGCCTGTAATCACATGTATCACTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAATTCTTGCCCCGTCTGCCGCCAGGAACTGCCACCACAGGGAATAGGTGGTGGGGGCGGGGGACATAGCACCAACGATCAAAACCGAAGCAACAGCTACAACAATGGGAGTGGCTCCCGGGTCAATCCAGGTAGACGCAATCCTTTCTCTTATCTGTGGCCATTTCGGTCATCTAGTTCTAACTCCAACCATGGCGGAGCAGGGAGCAGTGAACCGTCGAACCACCAAATGGGGTATTCAGGATATCGATACACTAATTCACCATGA

Protein sequence

MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQELDVVPGNPFDFFGLDHDEDRDHGLGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIGITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP
Homology
BLAST of PI0009288 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 5.8e-89
Identity = 184/321 (57.32%), Postives = 224/321 (69.78%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQELDVVPGNPFDFF----GLDHDEDR 60
           MS+ RNTHWCHRC+R+V L G++ VC  C  GF++ELD+   +PFD F    G+   +  
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQT 60

Query: 61  DHGLGAFSAFMRQRLAERN---DMRGRS-ESLFERSPGFGPLLIFGGQIPLR---NSRLE 120
              + AFS FMR RLAER+   ++RGR+  S  E  PG  PLLIFGGQ+P R   ++ +E
Sbjct: 61  FDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVE 120

Query: 121 ALFN-GAPGIGITRGDSGDYFIGPGLEELFEQLSA-NGHRGPPPASRSSIDAMPTVKITQ 180
           ALFN G+PGIGITRG++GDYF GPGLEELFEQLSA    RGPPPA RS+IDA+PT+KI Q
Sbjct: 121 ALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQ 180

Query: 181 RHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIG 240
           RHLR SDS+CPVCK++FELGSEA+QM CNH+YHSDCIVPWLVQHNSCPVCRQELP     
Sbjct: 181 RHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGP 240

Query: 241 GGGGGHSTNDQN-RSNSYNNGSGSRVNPG-RRNPFSYLWPFRSSSSNS----NHGGAGSS 293
                 +T  +N RS+S ++ S SR N   RRNPFS  WPFRSS S+S    N GG  +S
Sbjct: 241 SSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 300

BLAST of PI0009288 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.2e-80
Identity = 170/305 (55.74%), Postives = 207/305 (67.87%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQELDVVPGNPFDFFGLDHDEDRDHG- 60
           MS+ RNTHWCHRC+R+V LR RDAVC  C  GF++E+D+ P           D +RD   
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSR------AHRDVERDPTF 60

Query: 61  --LGAFSAFMRQRLAERN---DMRGRSESLFERS-PGFGPLLIFGGQIPLR-----NSRL 120
             + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS +
Sbjct: 61  DLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSV 120

Query: 121 EALFNG-APGIGITRG-DSGDYFIGPGLEELFEQLSA-NGHRGPPPASRSSIDAMPTVKI 180
           EA  NG APGIGI RG ++GDYF GPGLEEL EQLS+   HRGPPPA +SSIDA+PT+KI
Sbjct: 121 EAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKI 180

Query: 181 TQRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQG 240
           TQ+HL+ SDSHCPVCK++FEL SEA+QM C+H+YHSDCIVPWLVQHNSCPVCR+ELP + 
Sbjct: 181 TQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR- 240

Query: 241 IGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSN 290
                 G S++ Q+  N   NG   R N  RRN FS LWPFRSSSS+S      ++  + 
Sbjct: 241 ------GSSSSTQSSQNRSTNG---RENSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTAT 289

BLAST of PI0009288 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 7.7e-33
Identity = 102/312 (32.69%), Postives = 148/312 (47.44%), Query Frame = 0

Query: 9   WCHRCRRSVHL---RGRDAVCPNCNEGFIQE---------LDVVPGNPFDFFGL------ 68
           +C++C ++V +      D  CP CN+GF++E         L+  P +   FF +      
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPFST 81

Query: 69  ------DHDEDRDHGLGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQI-PL 128
                         G+   S F           + RS     +S  F P       +  L
Sbjct: 82  LLPLIFGSSAAAPSGMDFMSLF-----GPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTL 141

Query: 129 RNS--RLEALFNGAPGIGITR--GDSGDYFIGPGLEELFEQLSAN--GHRGPPPASRSSI 188
           R+S    E +    P     R  G+ GDYF GPGLE+L +QL+ N     G PPAS+S+I
Sbjct: 142 RSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAI 201

Query: 189 DAMPTVKITQRHLRSD-SHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVC 248
           DA+PTVK+T+  L+S+ + C VC ++FE GS+ +QM C H++H DC++PWL  HNSCPVC
Sbjct: 202 DALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVC 261

Query: 249 RQELPPQGIGGGGGGHSTNDQNRS-NSYNNGSGSRVNPGRRNP---FSYLWPFRSSSSNS 285
           R ELP             + +NRS  S  +G G     G++ P       WPFR    + 
Sbjct: 262 RFELPTD---------DPDYENRSQGSQGSGDGQGSVEGQQTPRFSIQLPWPFRRQDGSG 319

BLAST of PI0009288 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.0e-32
Identity = 100/298 (33.56%), Postives = 147/298 (49.33%), Query Frame = 0

Query: 9   WCHRCRRSVHLR----GRDAVCPNCNEGFIQELD-VVPGNPFDFFGLDHDEDRDHGL--- 68
           +C++C R+V++       D  CP CNEGF++E D   P     F   + +    H L   
Sbjct: 24  FCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFLT 83

Query: 69  --GAFSAFMRQRL-AERNDMRGRSESLFERSP--------------GFGPLLIFGGQI-P 128
               F++ +     +  +     S S+   +P               F P       +  
Sbjct: 84  LSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLND 143

Query: 129 LRNS--RLEALFNGAPGIGITR--GDSGDYFIGPGLEELFEQLSAN--GHRGPPPASRSS 188
           LR+S  ++E +    P     R   + GDYFIGPGLE+L +QL+ N     G PPAS+S+
Sbjct: 144 LRSSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSA 203

Query: 189 IDAMPTVKITQRHLRSD-SHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPV 248
           I+A+P V IT+ +L S+ + C VC + FE G+EA+QM C H+YH DC++PWL  HNSCPV
Sbjct: 204 IEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPV 263

Query: 249 CRQELP------PQGIGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRS 268
           CR ELP       + + G  G    ND + S   ++G    V    R   S  WPF++
Sbjct: 264 CRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFR--ISLPWPFQA 319

BLAST of PI0009288 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.7e-27
Identity = 82/272 (30.15%), Postives = 124/272 (45.59%), Query Frame = 0

Query: 7   THWCHRCRRSVHL---RGRDAV----CPNCNEGFIQELDVVPGNPFDFFGLDHDEDRDHG 66
           ++WC+ C R V +   +G   V    CP+C+ GFI++++       +       E R   
Sbjct: 17  SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATELTIPASTEVRSIN 76

Query: 67  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNS-----------RL 126
               S   R+R   R              P F P+++  G    R               
Sbjct: 77  NNRRSVIRRRRSGRR--------------PSFNPVIVLQGGAGEREEGEEGDAARDRRAF 136

Query: 127 EALFNGAPGIGI--TRGDSGDYFIGPGLEELFEQLS-------ANGHRGPPPASRSSIDA 186
           E  ++   G G+        +  +G G E L EQLS         G  G PPAS+S+I++
Sbjct: 137 EFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIES 196

Query: 187 MPTVKITQRHLRSDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQE 246
           +P V+I+  H+ S+++C VC E FE  +EAR+M C H++H DCIVPWL   NSCPVCR E
Sbjct: 197 LPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFE 256

Query: 247 LPPQGIGGGGGGHSTNDQNRSNSYNNGSGSRV 252
           LP +           N ++ +N  +N  G  +
Sbjct: 257 LPSE----------PNRRSNNNEEDNAVGMTI 264

BLAST of PI0009288 vs. ExPASy TrEMBL
Match: A0A0A0L0T9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G419610 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.1e-170
Identity = 290/299 (96.99%), Postives = 294/299 (98.33%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWC+RCRRSV LRGRDAVCPNCN GFIQEL D+VPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFE SPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELPPQGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299
           SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYT+SP
Sbjct: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTSSP 299

BLAST of PI0009288 vs. ExPASy TrEMBL
Match: A0A1S3C178 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495857 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.2e-168
Identity = 287/299 (95.99%), Postives = 292/299 (97.66%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWC+RCRRSV L GRDAVCPNCN GFIQEL D+VPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFE SPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LSANGHRGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELP QGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299
           SNSY+NGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP
Sbjct: 241 SNSYSNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299

BLAST of PI0009288 vs. ExPASy TrEMBL
Match: A0A5A7SIY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00710 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.2e-168
Identity = 287/299 (95.99%), Postives = 292/299 (97.66%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWC+RCRRSV L GRDAVCPNCN GFIQEL D+VPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFE SPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LSANGHRGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELP QGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299
           SNSY+NGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP
Sbjct: 241 SNSYSNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299

BLAST of PI0009288 vs. ExPASy TrEMBL
Match: A0A6J1DKF0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021749 PE=4 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 1.8e-154
Identity = 273/309 (88.35%), Postives = 286/309 (92.56%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MS+GRNTHWC+RCRRSVHLRGRDAVCPNCN GFIQEL D+VP NPFDFFGLDHDEDR  G
Sbjct: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FERSPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NG+RGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGG----GGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWLVQHNSCPVCRQELPPQGIGGG    GGGHSTN
Sbjct: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGAGGGHSTN 240

Query: 241 DQNR-SNSYNNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 299
            QNR S+SYNN S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300

BLAST of PI0009288 vs. ExPASy TrEMBL
Match: A0A6J1EAP6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432344 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.5e-151
Identity = 264/294 (89.80%), Postives = 277/294 (94.22%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MS+G+NTHWC+RCRRS+ L GRDAVCPNCN GFIQEL D+VPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSSGQNTHWCYRCRRSIRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGR+ESLFER+PGFGPLLIFGGQIPLRN RLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRAESLFERNPGFGPLLIFGGQIPLRNRRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGD GDYFIGPGLEELFEQLS NGHRGPPPASRS IDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDGGDYFIGPGLEELFEQLSENGHRGPPPASRSLIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQ +  GG GH+TN QNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQVM--GGAGHNTNSQNR 240

Query: 241 SNSY-NNGSG-SRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSG 292
           +NSY NNG+G SRVNPGRRNPFSYLWPFRSS+S+SNHGGAGSSE  NHQMGYSG
Sbjct: 241 NNSYNNNGNGSSRVNPGRRNPFSYLWPFRSSNSSSNHGGAGSSESRNHQMGYSG 292

BLAST of PI0009288 vs. NCBI nr
Match: XP_004144313.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_004144314.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_011653845.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_031739891.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >KGN54669.1 hypothetical protein Csa_012618 [Cucumis sativus])

HSP 1 Score: 609.0 bits (1569), Expect = 2.2e-170
Identity = 290/299 (96.99%), Postives = 294/299 (98.33%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWC+RCRRSV LRGRDAVCPNCN GFIQEL D+VPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFE SPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELPPQGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299
           SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYT+SP
Sbjct: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTSSP 299

BLAST of PI0009288 vs. NCBI nr
Match: XP_008455755.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455756.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455757.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0025786.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK09669.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 601.3 bits (1549), Expect = 4.6e-168
Identity = 287/299 (95.99%), Postives = 292/299 (97.66%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MSAGRNTHWC+RCRRSV L GRDAVCPNCN GFIQEL D+VPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFE SPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LSANGHRGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWL+QHNSCPVCRQELP QGIGGGGGGHSTNDQNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGIGGGGGGHSTNDQNR 240

Query: 241 SNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299
           SNSY+NGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP
Sbjct: 241 SNSYSNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGYRYTNSP 299

BLAST of PI0009288 vs. NCBI nr
Match: XP_038882645.1 (probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882646.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882647.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882648.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882649.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882650.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida])

HSP 1 Score: 581.6 bits (1498), Expect = 3.8e-162
Identity = 282/307 (91.86%), Postives = 288/307 (93.81%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MS GRNTHWC+RCRRSV L GRDAVCP+CN GFIQEL D+VPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSTGRNTHWCYRCRRSVRLHGRDAVCPSCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRL ERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLVERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTV ITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVNITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGI--------GGGGGG 240
           KEKFELG EARQM CNHMYHSDCIVPWLVQHNSCPVCRQELPPQGI        GGGGGG
Sbjct: 181 KEKFELGCEARQMPCNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGGGGG 240

Query: 241 HSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSG 299
           H+TN QNRSNSY NGSGSRVNPGRRNPFSYLWPFRSSSS+SNHGGAGSSEP+NHQMGYSG
Sbjct: 241 HNTNGQNRSNSY-NGSGSRVNPGRRNPFSYLWPFRSSSSSSNHGGAGSSEPTNHQMGYSG 300

BLAST of PI0009288 vs. NCBI nr
Match: XP_022154478.1 (probable E3 ubiquitin-protein ligase RHC1A [Momordica charantia])

HSP 1 Score: 555.1 bits (1429), Expect = 3.8e-154
Identity = 273/309 (88.35%), Postives = 286/309 (92.56%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MS+GRNTHWC+RCRRSVHLRGRDAVCPNCN GFIQEL D+VP NPFDFFGLDHDEDR  G
Sbjct: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FERSPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NG+RGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGG----GGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWLVQHNSCPVCRQELPPQGIGGG    GGGHSTN
Sbjct: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGAGGGHSTN 240

Query: 241 DQNR-SNSYNNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 299
            QNR S+SYNN S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300

BLAST of PI0009288 vs. NCBI nr
Match: XP_023517925.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_023517926.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545.8 bits (1405), Expect = 2.3e-151
Identity = 265/294 (90.14%), Postives = 277/294 (94.22%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQEL-DVVPGNPFDFFGLDHDEDRDHG 60
           MS+G+NTHWC+RCRRS+ L GRDAVCPNCN GFIQEL D+VPGNPFDFFGLDHDEDRDHG
Sbjct: 1   MSSGQNTHWCYRCRRSIRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESLFER+PGFGPLLIFGGQIPLRN RLEALFNGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFERNPGFGPLLIFGGQIPLRNRRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGD GDYFIGPGLEELFEQLS NGHRGPPPASRS IDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDGGDYFIGPGLEELFEQLSENGHRGPPPASRSLIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGHSTNDQNR 240
           KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQ +  GGGGH+TN QNR
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQVM--GGGGHNTNSQNR 240

Query: 241 SNSY-NNGSG-SRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSG 292
           +NSY NNG+G SRVNPGRRNPFSYLWPFRSS+S+SNHGGAGSSE  NH MGYSG
Sbjct: 241 NNSYNNNGNGSSRVNPGRRNPFSYLWPFRSSNSSSNHGGAGSSESRNHPMGYSG 292

BLAST of PI0009288 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 328.9 bits (842), Expect = 4.1e-90
Identity = 184/321 (57.32%), Postives = 224/321 (69.78%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQELDVVPGNPFDFF----GLDHDEDR 60
           MS+ RNTHWCHRC+R+V L G++ VC  C  GF++ELD+   +PFD F    G+   +  
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQT 60

Query: 61  DHGLGAFSAFMRQRLAERN---DMRGRS-ESLFERSPGFGPLLIFGGQIPLR---NSRLE 120
              + AFS FMR RLAER+   ++RGR+  S  E  PG  PLLIFGGQ+P R   ++ +E
Sbjct: 61  FDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVE 120

Query: 121 ALFN-GAPGIGITRGDSGDYFIGPGLEELFEQLSA-NGHRGPPPASRSSIDAMPTVKITQ 180
           ALFN G+PGIGITRG++GDYF GPGLEELFEQLSA    RGPPPA RS+IDA+PT+KI Q
Sbjct: 121 ALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQ 180

Query: 181 RHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIG 240
           RHLR SDS+CPVCK++FELGSEA+QM CNH+YHSDCIVPWLVQHNSCPVCRQELP     
Sbjct: 181 RHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGP 240

Query: 241 GGGGGHSTNDQN-RSNSYNNGSGSRVNPG-RRNPFSYLWPFRSSSSNS----NHGGAGSS 293
                 +T  +N RS+S ++ S SR N   RRNPFS  WPFRSS S+S    N GG  +S
Sbjct: 241 SSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 300

BLAST of PI0009288 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 328.9 bits (842), Expect = 4.1e-90
Identity = 184/321 (57.32%), Postives = 224/321 (69.78%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQELDVVPGNPFDFF----GLDHDEDR 60
           MS+ RNTHWCHRC+R+V L G++ VC  C  GF++ELD+   +PFD F    G+   +  
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQT 60

Query: 61  DHGLGAFSAFMRQRLAERN---DMRGRS-ESLFERSPGFGPLLIFGGQIPLR---NSRLE 120
              + AFS FMR RLAER+   ++RGR+  S  E  PG  PLLIFGGQ+P R   ++ +E
Sbjct: 61  FDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVE 120

Query: 121 ALFN-GAPGIGITRGDSGDYFIGPGLEELFEQLSA-NGHRGPPPASRSSIDAMPTVKITQ 180
           ALFN G+PGIGITRG++GDYF GPGLEELFEQLSA    RGPPPA RS+IDA+PT+KI Q
Sbjct: 121 ALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQ 180

Query: 181 RHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIG 240
           RHLR SDS+CPVCK++FELGSEA+QM CNH+YHSDCIVPWLVQHNSCPVCRQELP     
Sbjct: 181 RHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGP 240

Query: 241 GGGGGHSTNDQN-RSNSYNNGSGSRVNPG-RRNPFSYLWPFRSSSSNS----NHGGAGSS 293
                 +T  +N RS+S ++ S SR N   RRNPFS  WPFRSS S+S    N GG  +S
Sbjct: 241 SSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 300

BLAST of PI0009288 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 328.9 bits (842), Expect = 4.1e-90
Identity = 184/321 (57.32%), Postives = 224/321 (69.78%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQELDVVPGNPFDFF----GLDHDEDR 60
           MS+ RNTHWCHRC+R+V L G++ VC  C  GF++ELD+   +PFD F    G+   +  
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQT 60

Query: 61  DHGLGAFSAFMRQRLAERN---DMRGRS-ESLFERSPGFGPLLIFGGQIPLR---NSRLE 120
              + AFS FMR RLAER+   ++RGR+  S  E  PG  PLLIFGGQ+P R   ++ +E
Sbjct: 61  FDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVE 120

Query: 121 ALFN-GAPGIGITRGDSGDYFIGPGLEELFEQLSA-NGHRGPPPASRSSIDAMPTVKITQ 180
           ALFN G+PGIGITRG++GDYF GPGLEELFEQLSA    RGPPPA RS+IDA+PT+KI Q
Sbjct: 121 ALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQ 180

Query: 181 RHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIG 240
           RHLR SDS+CPVCK++FELGSEA+QM CNH+YHSDCIVPWLVQHNSCPVCRQELP     
Sbjct: 181 RHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGP 240

Query: 241 GGGGGHSTNDQN-RSNSYNNGSGSRVNPG-RRNPFSYLWPFRSSSSNS----NHGGAGSS 293
                 +T  +N RS+S ++ S SR N   RRNPFS  WPFRSS S+S    N GG  +S
Sbjct: 241 SSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 300

BLAST of PI0009288 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.6e-81
Identity = 170/305 (55.74%), Postives = 207/305 (67.87%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQELDVVPGNPFDFFGLDHDEDRDHG- 60
           MS+ RNTHWCHRC+R+V LR RDAVC  C  GF++E+D+ P           D +RD   
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSR------AHRDVERDPTF 60

Query: 61  --LGAFSAFMRQRLAERN---DMRGRSESLFERS-PGFGPLLIFGGQIPLR-----NSRL 120
             + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS +
Sbjct: 61  DLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSV 120

Query: 121 EALFNG-APGIGITRG-DSGDYFIGPGLEELFEQLSA-NGHRGPPPASRSSIDAMPTVKI 180
           EA  NG APGIGI RG ++GDYF GPGLEEL EQLS+   HRGPPPA +SSIDA+PT+KI
Sbjct: 121 EAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKI 180

Query: 181 TQRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQG 240
           TQ+HL+ SDSHCPVCK++FEL SEA+QM C+H+YHSDCIVPWLVQHNSCPVCR+ELP + 
Sbjct: 181 TQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR- 240

Query: 241 IGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSN 290
                 G S++ Q+  N   NG   R N  RRN FS LWPFRSSSS+S      ++  + 
Sbjct: 241 ------GSSSSTQSSQNRSTNG---RENSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTAT 289

BLAST of PI0009288 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.6e-81
Identity = 170/305 (55.74%), Postives = 207/305 (67.87%), Query Frame = 0

Query: 1   MSAGRNTHWCHRCRRSVHLRGRDAVCPNCNEGFIQELDVVPGNPFDFFGLDHDEDRDHG- 60
           MS+ RNTHWCHRC+R+V LR RDAVC  C  GF++E+D+ P           D +RD   
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSR------AHRDVERDPTF 60

Query: 61  --LGAFSAFMRQRLAERN---DMRGRSESLFERS-PGFGPLLIFGGQIPLR-----NSRL 120
             + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS +
Sbjct: 61  DLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSV 120

Query: 121 EALFNG-APGIGITRG-DSGDYFIGPGLEELFEQLSA-NGHRGPPPASRSSIDAMPTVKI 180
           EA  NG APGIGI RG ++GDYF GPGLEEL EQLS+   HRGPPPA +SSIDA+PT+KI
Sbjct: 121 EAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKI 180

Query: 181 TQRHLR-SDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQG 240
           TQ+HL+ SDSHCPVCK++FEL SEA+QM C+H+YHSDCIVPWLVQHNSCPVCR+ELP + 
Sbjct: 181 TQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSR- 240

Query: 241 IGGGGGGHSTNDQNRSNSYNNGSGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSN 290
                 G S++ Q+  N   NG   R N  RRN FS LWPFRSSSS+S      ++  + 
Sbjct: 241 ------GSSSSTQSSQNRSTNG---RENSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTAT 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O221975.8e-8957.32Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q94AK42.2e-8055.74E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
Q8LPN77.7e-3332.69E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
P0CH301.0e-3233.56E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q940T53.7e-2730.15E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L0T91.1e-17096.99RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G419610 PE... [more]
A0A1S3C1782.2e-16895.99RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495857 PE=4 ... [more]
A0A5A7SIY22.2e-16895.99RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1DKF01.8e-15488.35RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102174... [more]
A0A6J1EAP62.5e-15189.80RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432344... [more]
Match NameE-valueIdentityDescription
XP_004144313.12.2e-17096.99probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_004144314.1 pro... [more]
XP_008455755.14.6e-16895.99PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455756.1... [more]
XP_038882645.13.8e-16291.86probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882646.1 p... [more]
XP_022154478.13.8e-15488.35probable E3 ubiquitin-protein ligase RHC1A [Momordica charantia][more]
XP_023517925.12.3e-15190.14probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_0235... [more]
Match NameE-valueIdentityDescription
AT2G40830.24.1e-9057.32RING-H2 finger C1A [more]
AT2G40830.14.1e-9057.32RING-H2 finger C1A [more]
AT2G40830.34.1e-9057.32RING-H2 finger C1A [more]
AT3G56580.21.6e-8155.74RING/U-box superfamily protein [more]
AT3G56580.11.6e-8155.74RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 176..216
e-value: 1.0E-6
score: 38.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 175..217
e-value: 2.1E-10
score: 40.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 176..217
score: 11.972923
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 139..223
e-value: 9.8E-22
score: 78.4
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 6..37
e-value: 4.2E-11
score: 42.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 230..254
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 262..298
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..298
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 2..283
NoneNo IPR availablePANTHERPTHR15710:SF86E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATEDcoord: 2..283
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 176..217
e-value: 1.7622E-23
score: 88.5288
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 156..223

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009288.1PI0009288.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity