PI0009268 (gene) Melon (PI 482460) v1

Overview
NamePI0009268
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRop guanine nucleotide exchange factor 12-like
Locationchr01: 27731741 .. 27735384 (+)
RNA-Seq ExpressionPI0009268
SyntenyPI0009268
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAGATGGTTATGAATGAAGGATGGTTGTGTTTCCAAATCTTTTTCAATTTTAGCAAAGTTTGACCTAAACCATCCAATTGAAGCACTGCTGAAAGAAAAAAACAAAGCAAATTCTTCCACCAAAAAACAAAAAGTAAGTTCAAAACATTTCTTTCTTTCTTTCTTTGTCTAACCAAATCATCCTTTTCTTAAGATGAACCTCACATTGCATCAATCACCTTCTCAAAAATAGAAGGAGAAAAGGAAAGATCAATCCAATAACCAAAAAGTAAAACTAGAGAGAGAGTAAATAAAACAAAAACTTTCCTTATTCTTCAGTTCAAAGGAGGTGAAGAGAAGCAAAACAAGAATATCCTAATCCACTTTTTTTTTTCTTTCTAGTTCTTCTCTAAATCCTGCTTGCTTCTCTTCCTCCTTTTTCTCAATTCTCTTCATTCTCTCTCTATGCTGTCTGAAATCCACAAGGCATTCATCACCACCCAGTTGCCGGTTTGTTGTCTCTTGTGTTTTCAGTCCCACGTTTCGTCAAGAAACTGAGAATGGTTCGTGCTATGGAGCAAGAGCACGAAGGTTTCAGGTCCAGATTCTTTCATTTCAGAGGAACGCATGAGAATACAGGCAGACATGCCAAGAGCTTGAGTGTGGAGAGTGCCAATATATTTGACACTGTCATTGAAGATGATTTGTCGACGTCGAGAAGCCAGGGATCAACACCGGTTCACAACAACTCTTCTGAGAAACACCAAGTTGCGCCACCACTGGCACCGTCCAATCGATCTGAAAGGGCTCCGAAGCCAAAGTTGAGCAGCAAAGACGATGATCCTGCCGCTGAAAAAGAAGCCAAGGATAAGATGGCCGTAGGTTTGTGTTCCTTTTTAGTCCTTAAGACTTTCAAAACAAAAATTCTGATCCTTGTAACTGTTTTATAATCTAATATCTACTTAACCAAGACCTTACTTTAATCAACTTCAGCACATGCCACGTTTGCACACATTCTTGTTAATTTTAAAGACAAAAATAGTTATTGTTTCAAGCCTTGGGACAAAAATAGACATTTTAGAGGTTGAAGGGCTAAAACAGAGCAAACATAAAGTTTGAGGACCAAAATGGACTAAAATAGTTGAAGGTTAGGTATCCAAATGAGGTCATTAGTTACTCTAGGTGGAAGGCTTTTTATTTGATTCTGCAATTAATTTCCTCTTCCAATTGGGTTATAATTCAGAAATGGAACAGATGAAAGAGAGGTTTGCAAAGTTACTCTTGGGGGAAGACATGTCTGGGGGAGGGAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCAATCACAAACCTTGCTGGTACGTCCCTAAAATTTCTTACATTGTACCCTTGGGAAAATTTTCCCTTAGTTTTCGGCCTCAAAATCCAGCTGAACCAGTACAGAAATTTTTGTTAGATATAAATTTTGTCTTTCAATTTTGCAGCTTCTGTTTTTGGAGAACAATGGCGTCTTGAGCCAATGTCAGCAGAAAGAAAAGCAAGATGGAGAAAAGAAATCGATTTGCTCTTATCCGTTACAGATTACATTGTAGAGTTTGTTCCTTCACAACAAAAATCCAAGGATGGAACGAATATGGAGGTAACAATCCATTTGCAACTCTGTAATTAAAACCCATTGATATGAGTCTCACAAGCTTAGATTAGGAGAATATTACTCCTTTCAATTCATTAATACCCCGACCTTAAGCCCAATGCTCCTACCTTTCTTTTTCTATCTTACAAGTAAGGTTTATTATTGTTATAGATCATGGTGACACGGCAACGAAATGATTTGCACTTGAATATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGTAAGAACTATGGCTTAAATCTACTTTATTTTTTCTTGTCTTTTCTGCTTCAGTCGAACGGATCCTGTGTGATATTGGGTCTGCCATGACAAAATGCAGGATTGTCTGGATAATTTTAAAGACCAAAATGAGTTCTACTACGTATCAAGAGATGCCGATGACTTGGAGAAAGGAAATAACAAAAGGAAAGATGATAAATGGTGGCTACCTACGGCCAAAGTTCCTCCAAATGGTTTATCAGACATGTCGAGGAAGTTTTTGCAGTACCAGAAGGATTGTGTCAACCAAGTCCTCAAAGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATCCCTGAAGACTACATTGAATCTCTCCCTAAAGTAGGCAAAGTCAATTATGTTTGTAACAATTTCAAAATGAATCTTCAATCAAAATTAAGTTTTGTTTGTGGTTTTACAGAATGGAAGGGCAAGCCTAGGGGATTCCATTTACAAAAGTATAACAGTTGAGTTCTTTGATCCAGATCAGTTTCTCTCCAGCATGGACCTAACATCAGAGCATAAAATCCTAGATCTCAAAAACAGAATTGAAGCCTCCATAGTGATTTGGAGAAGGAAGATGAACCAGAAAGATGGAAAATCTACTTGGGGTTCAGCAGTGAGTTTGGAAAAGAGAGAACTATTTGAAGAAAGAGCAGAAACCATCTTGCTAATACTTAAGCACCGTTTCCCTGGAATTCCACAATCTTCACTCGACATCAGCAAAATTCAGTTCAACCGGGTAAAAGAACGCTCCTTCCTTGTCTATAATCTCATCTAAAATTATTAACAATCCAAACACAATTTTTAGAAACTAAAATCAATGCTTCTCAACTTTAGGCTACATAAACAGATACTTATTATAATTCTGCCCAACAATATCTTCTTCTAAATGAGATGATATATGTTTTTAGCCCATGTAACCAAATAGGAAATAACAATCCTGTACCCTTTTCTTTGACAGTGTGAGATCATAAAACACTGTCTAAAATAACATGTCCAAACATATTGTAAATACCTAGTTTCATAGTGTGATGTGATGACTCACCACAAACAAACTTACTCTTGGGTGAACTGCAGGACGTGGGGCACGCCGTTTTAGAGAGCTACTCCAGAATACTGGAAAGCTTAGCCTTCACAGTGATGTCACGAATTGAAGACGTACTCCACGCCGATGGGTTAACTCAGAACCCATCACAAATAGCAACAAGGAGGAAACCGACGAGCGAACCCCCAATGGAAAAGTCAGAAGAGTTGAACAACAACGGCCCAGAAACGCCGGCTTCAATGACGCTGTTGGATTTCATGGGGTGGGGACAGGATCAAAACGAGGCGGAGATGAAGAAGGAATCGTTTGGGAATTCAGATGATTTAAACGCGGATTCAGATCTGAAACAAGGGAATAAGCCAGGGAATATAGTGACGAACAAGAAGGTTTCGTACCTGGAGAATTTGAGCGCTGTAAGAAGTCCAACGGCTCGCCATTGAAGAAGAAGAAGAAGAATAGATAGAGAGATGATTTGGAGGGAAAATTCCATTATTCTCTGTTATATACATTCCTTTTGTGTAAATAGGAAGAGGAAGAAGAAGAATGAGATCAACCCTTTTTTTTTCTTTTTTTAATTTGGATTTTGGAATCACAACTCTTTGTGTTTGTGTAAAACCAAAATTGTTCTTATGTATCATTTGTATCAATCAATGTAGTCATTTTAGATTCATACATTCAAAAATATTAAGTCCATTCCCAACAA

mRNA sequence

GAGAAGATGGTTATGAATGAAGGATGGTTGTGTTTCCAAATCTTTTTCAATTTTAGCAAAGTTTGACCTAAACCATCCAATTGAAGCACTGCTGAAAGAAAAAAACAAAGCAAATTCTTCCACCAAAAAACAAAAAGTAAGTTCAAAACATTTCTTTCTTTCTTTCTTTGTCTAACCAAATCATCCTTTTCTTAAGATGAACCTCACATTGCATCAATCACCTTCTCAAAAATAGAAGGAGAAAAGGAAAGATCAATCCAATAACCAAAAAGTAAAACTAGAGAGAGAGTAAATAAAACAAAAACTTTCCTTATTCTTCAGTTCAAAGGAGGTGAAGAGAAGCAAAACAAGAATATCCTAATCCACTTTTTTTTTTCTTTCTAGTTCTTCTCTAAATCCTGCTTGCTTCTCTTCCTCCTTTTTCTCAATTCTCTTCATTCTCTCTCTATGCTGTCTGAAATCCACAAGGCATTCATCACCACCCAGTTGCCGGTTTGTTGTCTCTTGTGTTTTCAGTCCCACGTTTCGTCAAGAAACTGAGAATGGTTCGTGCTATGGAGCAAGAGCACGAAGGTTTCAGGTCCAGATTCTTTCATTTCAGAGGAACGCATGAGAATACAGGCAGACATGCCAAGAGCTTGAGTGTGGAGAGTGCCAATATATTTGACACTGTCATTGAAGATGATTTGTCGACGTCGAGAAGCCAGGGATCAACACCGGTTCACAACAACTCTTCTGAGAAACACCAAGTTGCGCCACCACTGGCACCGTCCAATCGATCTGAAAGGGCTCCGAAGCCAAAGTTGAGCAGCAAAGACGATGATCCTGCCGCTGAAAAAGAAGCCAAGGATAAGATGGCCGTAGAAATGGAACAGATGAAAGAGAGGTTTGCAAAGTTACTCTTGGGGGAAGACATGTCTGGGGGAGGGAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCAATCACAAACCTTGCTGCTTCTGTTTTTGGAGAACAATGGCGTCTTGAGCCAATGTCAGCAGAAAGAAAAGCAAGATGGAGAAAAGAAATCGATTTGCTCTTATCCGTTACAGATTACATTGTAGAGTTTGTTCCTTCACAACAAAAATCCAAGGATGGAACGAATATGGAGATCATGGTGACACGGCAACGAAATGATTTGCACTTGAATATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGATTGTCTGGATAATTTTAAAGACCAAAATGAGTTCTACTACGTATCAAGAGATGCCGATGACTTGGAGAAAGGAAATAACAAAAGGAAAGATGATAAATGGTGGCTACCTACGGCCAAAGTTCCTCCAAATGGTTTATCAGACATGTCGAGGAAGTTTTTGCAGTACCAGAAGGATTGTGTCAACCAAGTCCTCAAAGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATCCCTGAAGACTACATTGAATCTCTCCCTAAAAATGGAAGGGCAAGCCTAGGGGATTCCATTTACAAAAGTATAACAGTTGAGTTCTTTGATCCAGATCAGTTTCTCTCCAGCATGGACCTAACATCAGAGCATAAAATCCTAGATCTCAAAAACAGAATTGAAGCCTCCATAGTGATTTGGAGAAGGAAGATGAACCAGAAAGATGGAAAATCTACTTGGGGTTCAGCAGTGAGTTTGGAAAAGAGAGAACTATTTGAAGAAAGAGCAGAAACCATCTTGCTAATACTTAAGCACCGTTTCCCTGGAATTCCACAATCTTCACTCGACATCAGCAAAATTCAGTTCAACCGGGACGTGGGGCACGCCGTTTTAGAGAGCTACTCCAGAATACTGGAAAGCTTAGCCTTCACAGTGATGTCACGAATTGAAGACGTACTCCACGCCGATGGGTTAACTCAGAACCCATCACAAATAGCAACAAGGAGGAAACCGACGAGCGAACCCCCAATGGAAAAGTCAGAAGAGTTGAACAACAACGGCCCAGAAACGCCGGCTTCAATGACGCTGTTGGATTTCATGGGGTGGGGACAGGATCAAAACGAGGCGGAGATGAAGAAGGAATCGTTTGGGAATTCAGATGATTTAAACGCGGATTCAGATCTGAAACAAGGGAATAAGCCAGGGAATATAGTGACGAACAAGAAGGTTTCGTACCTGGAGAATTTGAGCGCTGTAAGAAGTCCAACGGCTCGCCATTGAAGAAGAAGAAGAAGAATAGATAGAGAGATGATTTGGAGGGAAAATTCCATTATTCTCTGTTATATACATTCCTTTTGTGTAAATAGGAAGAGGAAGAAGAAGAATGAGATCAACCCTTTTTTTTTCTTTTTTTAATTTGGATTTTGGAATCACAACTCTTTGTGTTTGTGTAAAACCAAAATTGTTCTTATGTATCATTTGTATCAATCAATGTAGTCATTTTAGATTCATACATTCAAAAATATTAAGTCCATTCCCAACAA

Coding sequence (CDS)

ATGGTTCGTGCTATGGAGCAAGAGCACGAAGGTTTCAGGTCCAGATTCTTTCATTTCAGAGGAACGCATGAGAATACAGGCAGACATGCCAAGAGCTTGAGTGTGGAGAGTGCCAATATATTTGACACTGTCATTGAAGATGATTTGTCGACGTCGAGAAGCCAGGGATCAACACCGGTTCACAACAACTCTTCTGAGAAACACCAAGTTGCGCCACCACTGGCACCGTCCAATCGATCTGAAAGGGCTCCGAAGCCAAAGTTGAGCAGCAAAGACGATGATCCTGCCGCTGAAAAAGAAGCCAAGGATAAGATGGCCGTAGAAATGGAACAGATGAAAGAGAGGTTTGCAAAGTTACTCTTGGGGGAAGACATGTCTGGGGGAGGGAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCAATCACAAACCTTGCTGCTTCTGTTTTTGGAGAACAATGGCGTCTTGAGCCAATGTCAGCAGAAAGAAAAGCAAGATGGAGAAAAGAAATCGATTTGCTCTTATCCGTTACAGATTACATTGTAGAGTTTGTTCCTTCACAACAAAAATCCAAGGATGGAACGAATATGGAGATCATGGTGACACGGCAACGAAATGATTTGCACTTGAATATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGATTGTCTGGATAATTTTAAAGACCAAAATGAGTTCTACTACGTATCAAGAGATGCCGATGACTTGGAGAAAGGAAATAACAAAAGGAAAGATGATAAATGGTGGCTACCTACGGCCAAAGTTCCTCCAAATGGTTTATCAGACATGTCGAGGAAGTTTTTGCAGTACCAGAAGGATTGTGTCAACCAAGTCCTCAAAGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATCCCTGAAGACTACATTGAATCTCTCCCTAAAAATGGAAGGGCAAGCCTAGGGGATTCCATTTACAAAAGTATAACAGTTGAGTTCTTTGATCCAGATCAGTTTCTCTCCAGCATGGACCTAACATCAGAGCATAAAATCCTAGATCTCAAAAACAGAATTGAAGCCTCCATAGTGATTTGGAGAAGGAAGATGAACCAGAAAGATGGAAAATCTACTTGGGGTTCAGCAGTGAGTTTGGAAAAGAGAGAACTATTTGAAGAAAGAGCAGAAACCATCTTGCTAATACTTAAGCACCGTTTCCCTGGAATTCCACAATCTTCACTCGACATCAGCAAAATTCAGTTCAACCGGGACGTGGGGCACGCCGTTTTAGAGAGCTACTCCAGAATACTGGAAAGCTTAGCCTTCACAGTGATGTCACGAATTGAAGACGTACTCCACGCCGATGGGTTAACTCAGAACCCATCACAAATAGCAACAAGGAGGAAACCGACGAGCGAACCCCCAATGGAAAAGTCAGAAGAGTTGAACAACAACGGCCCAGAAACGCCGGCTTCAATGACGCTGTTGGATTTCATGGGGTGGGGACAGGATCAAAACGAGGCGGAGATGAAGAAGGAATCGTTTGGGAATTCAGATGATTTAAACGCGGATTCAGATCTGAAACAAGGGAATAAGCCAGGGAATATAGTGACGAACAAGAAGGTTTCGTACCTGGAGAATTTGAGCGCTGTAAGAAGTCCAACGGCTCGCCATTGA

Protein sequence

MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPVHNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEASIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEELNNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKVSYLENLSAVRSPTARH
Homology
BLAST of PI0009268 vs. ExPASy Swiss-Prot
Match: Q9CA89 (Rop guanine nucleotide exchange factor 12 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF12 PE=1 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 4.9e-174
Identity = 354/564 (62.77%), Postives = 414/564 (73.40%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFR---GTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGS 60
           MVRA EQE E +RSR F+F+     + +  RH KSLSVE+      + E    +  ++  
Sbjct: 1   MVRASEQEQETYRSRLFNFKWRNNDNNSATRHNKSLSVETG-----LDEAATGSHDAEPL 60

Query: 61  TPVHNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFA 120
           T +H +        PPL+ S   E      L++     AA +  + ++  +MEQMKERF+
Sbjct: 61  TIIHPSQ------GPPLSRSAADEAV----LAAL----AASQARERQLLADMEQMKERFS 120

Query: 121 KLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSV 180
           KLLLGED SGGGKGVSSALALSNAITNLAASVFGEQ RLEPM AER+ARWRKEID LLSV
Sbjct: 121 KLLLGEDNSGGGKGVSSALALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLSV 180

Query: 181 TDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYY 240
           TDY+VEF PSQQK+KDGTNMEIM TRQR DLH+NIPAL+KLDAMLIDCL+NFKDQ+EF Y
Sbjct: 181 TDYVVEFAPSQQKNKDGTNMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFSY 240

Query: 241 VSRDADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQ 300
           +S+D+ DL+    KR D+KWW+PT KVPP+GLS+ SR+FLQYQKDCVNQVLKAAMAINAQ
Sbjct: 241 ISKDSPDLD---GKRNDEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINAQ 300

Query: 301 IISEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRI 360
           ++ EMEIPE YI+SLPKNGRASLGD +YK+ITV+FFDPDQFLSSMD++SEHKI+DLKNRI
Sbjct: 301 VLFEMEIPESYIDSLPKNGRASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNRI 360

Query: 361 EASIVIWRRKMNQKDGKST--WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDIS 420
           EASI+IW+RKM  KD KS+  W S VSLEKRE+FEERAETILLILK R+PGI QSSLDIS
Sbjct: 361 EASIIIWKRKMVYKDNKSSAPWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDIS 420

Query: 421 KIQFNRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPME 480
           KIQFN DVG AVLESYSRILESLA+TV+SRI+DVL AD           R       P+E
Sbjct: 421 KIQFNEDVGQAVLESYSRILESLAYTVLSRIDDVLEAD-----------RAGNKRNTPLE 480

Query: 481 KSEELNNNGPETPASMTLLDFMGWGQDQ---NEAEMKKESFGNSDDLNADSDLKQGNKPG 540
             EE          SMTL DFMGW  DQ    E E KK       DL  D  +K+     
Sbjct: 481 AEEE------TLVGSMTLSDFMGWDFDQAANAELESKK-------DLPDDPLIKE---KL 515

Query: 541 NIVTNKKVSYLENLSAVRSPTARH 557
           ++VT KK SYLE L  V+SPTARH
Sbjct: 541 SVVTTKKTSYLETLGGVKSPTARH 515

BLAST of PI0009268 vs. ExPASy Swiss-Prot
Match: Q9M811 (Rop guanine nucleotide exchange factor 11 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF11 PE=1 SV=2)

HSP 1 Score: 605.9 bits (1561), Expect = 4.6e-172
Identity = 348/559 (62.25%), Postives = 407/559 (72.81%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENT-GRHAKSLSVESANIFD--TVIEDDLSTSRSQGS 60
           MV+AMEQE E ++SR FHF+  +EN+  RH KS S + A   D    ++DD +      S
Sbjct: 1   MVKAMEQEQETYKSRLFHFKNMNENSASRHVKSWSSDCAMRMDGSDNLDDDDNDMMMFRS 60

Query: 61  TPVHNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFA 120
            P    S ++  +       NR+++   P++SS D       EA   +   MEQMKE+F+
Sbjct: 61  QPGKCGSVDRPSLPIGGVTPNRNDKL--PRVSSSD-----SMEALIILQAAMEQMKEKFS 120

Query: 121 KLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSV 180
           KLLLGEDMSGGGKGVSSALALSNAITNLAAS FGEQ RLEPM+ +RK RWR+EI  L+SV
Sbjct: 121 KLLLGEDMSGGGKGVSSALALSNAITNLAASAFGEQRRLEPMAVDRKTRWRREIGWLISV 180

Query: 181 TDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYY 240
            DYIVEF P+QQ +KDGT+ME+M TRQR DL  NIPAL+KLDAML+DCLD FKDQ+EFYY
Sbjct: 181 ADYIVEFAPTQQTNKDGTSMEVMSTRQRTDLLCNIPALKKLDAMLLDCLDKFKDQDEFYY 240

Query: 241 VSRDADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQ 300
           V +D+ D       R D+KWWLP  KVPPNGLS++SR+FLQ QK+CVNQVLKAAMAINAQ
Sbjct: 241 VKKDSPD---SCETRNDEKWWLPAVKVPPNGLSEISRRFLQSQKECVNQVLKAAMAINAQ 300

Query: 301 IISEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRI 360
           ++SEMEIPE Y+ESLPKNGRASLGD IY+ ITVE FD DQFL  MDL+SEHKILDLKNRI
Sbjct: 301 VLSEMEIPESYLESLPKNGRASLGDVIYRMITVEMFDADQFLIEMDLSSEHKILDLKNRI 360

Query: 361 EASIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKI 420
           EASIVIW+RKM QKD KS WGS VS+EKRE FEERAETILL+LK  FPGI QSSLDISKI
Sbjct: 361 EASIVIWKRKMVQKDTKSPWGSTVSIEKREQFEERAETILLLLKQGFPGISQSSLDISKI 420

Query: 421 QFNRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKS 480
           QFNRDVG A+LESYSR+LESLA TVMSRIEDVL+AD LTQ P+  A  +   S    EK 
Sbjct: 421 QFNRDVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSLKENEKL 480

Query: 481 EELNNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTN 540
            E   +  E  AS TL D M WG   N  EMKKESF    +    S +      G +  N
Sbjct: 481 REERLSFTEDMASGTLSDVMQWGNKNN--EMKKESFFGDREKPLLSKV-----TGIMTNN 540

Query: 541 KKVSYLENLSAVRSPTARH 557
           KK SYL+NL A+RSPTAR+
Sbjct: 541 KKSSYLDNLGAMRSPTARY 542

BLAST of PI0009268 vs. ExPASy Swiss-Prot
Match: Q9LV40 (Rho guanine nucleotide exchange factor 8 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF8 PE=1 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 3.7e-158
Identity = 317/555 (57.12%), Postives = 387/555 (69.73%), Query Frame = 0

Query: 14  SRFFHFRGTHENTG-RHAKSLSVESANIFDTVIEDDLSTSRSQGSTPVHNNSSEKHQVAP 73
           S+ F+F+   +++  +  +S ++   N    ++E +   S++  S       S    ++P
Sbjct: 12  SKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFVESPVESSLPMISP 71

Query: 74  PLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLLLGEDMSGGGKGV 133
              P  RSER                         +ME MK+RFAKLLLGEDMSGGGKGV
Sbjct: 72  LTRPGKRSER----------------------QQADMEMMKDRFAKLLLGEDMSGGGKGV 131

Query: 134 SSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDYIVEFVPSQQKSK 193
           SSALALSNAITNLAAS+FGEQ +L+PM  +R+ARW+KEID LLSVTD+IVEFVPSQQ SK
Sbjct: 132 SSALALSNAITNLAASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSK 191

Query: 194 DGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDLEKGNNKR 253
           DG   EIMVTRQR DL +NIPALRKLDAMLID LDNF+  NEF+YVSRD+++ ++  N R
Sbjct: 192 DGVCTEIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDR 251

Query: 254 KDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESL 313
            +DKWWLP  KVPP GLS+ SR+ L +QKD V QV KAAMAINAQ++SEMEIPE YI+SL
Sbjct: 252 TNDKWWLPPVKVPPGGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSL 311

Query: 314 PKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEASIVIWRRKMNQKD 373
           PKNGRASLGDSIYKSIT E+FDP+QFL+ +D+++EHK+LDLKNRIEAS+VIW+RK++ KD
Sbjct: 312 PKNGRASLGDSIYKSITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKD 371

Query: 374 GKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAVLESYS 433
            KS+WGSAVSLEKRELFEERAETIL++LK +FPG+PQSSLDISKIQFN+DVG AVLESYS
Sbjct: 372 TKSSWGSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYS 431

Query: 434 RILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL--NNNGPET--- 493
           RILESLA+TVMSRIEDVL+ D L    + +A             SE    +N+G E    
Sbjct: 432 RILESLAYTVMSRIEDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKH 491

Query: 494 -PASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNI--VTNKKVSY-- 553
            P S TLLDFMGW                      D+  K G+KP     +T KK+SY  
Sbjct: 492 DPHSKTLLDFMGWN---------------------DNSSKGGDKPTKSPNLTPKKLSYLE 523

Query: 554 -LENLSAVRSPTARH 557
            LENL+  RSP  RH
Sbjct: 552 KLENLNGFRSPKDRH 523

BLAST of PI0009268 vs. ExPASy Swiss-Prot
Match: Q9SVQ3 (Rop guanine nucleotide exchange factor 9 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF9 PE=1 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.1e-154
Identity = 305/531 (57.44%), Postives = 380/531 (71.56%), Query Frame = 0

Query: 45  IEDDLSTSRSQGSTPVHNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAK-- 104
           +E  +S + S     + ++S  K Q  P LA +   E   +  L     + +    ++  
Sbjct: 5   LERGISITSSFNLDRMFDSSPGKEQQQPHLAETTMPESQTQDSLGGSPVETSRPMTSRLI 64

Query: 105 ----DKMAVEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEP 164
               DK   E E MK+RF KLLLGEDMSGGGKGVSSALALSNAITNLAAS+FGEQ +L+P
Sbjct: 65  SRRQDKQQSETEMMKDRFTKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQP 124

Query: 165 MSAERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKL 224
           M+ +R+ARW+KEID LLSVTD+IVEFVPSQQ SK+G   EIMVTRQR DL +NIPALRKL
Sbjct: 125 MAPDRRARWKKEIDWLLSVTDHIVEFVPSQQISKEGVCTEIMVTRQRGDLLMNIPALRKL 184

Query: 225 DAMLIDCLDNFKDQNEFYYVSRDADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQ 284
           DAMLID LDNF+  NEF+YVSRD+++ ++  N+R  DKWWLP  KVPPNGLS+ +R+ L 
Sbjct: 185 DAMLIDTLDNFRGHNEFWYVSRDSEEGKQARNERTKDKWWLPPVKVPPNGLSESARRMLH 244

Query: 285 YQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQF 344
           +QKD V+QV KAAMAINAQ++SEM IP+ YIESLPKNGR SLGDS+YKSIT E+FDP+QF
Sbjct: 245 FQKDSVSQVQKAAMAINAQVLSEMAIPDSYIESLPKNGRVSLGDSLYKSITEEWFDPEQF 304

Query: 345 LSSMDLTSEHKILDLKNRIEASIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILL 404
           LS++DL++EHK+LD+KNRIEASIVIW+RK++ KD KS+WGSAVSLEKRELFEERAETIL+
Sbjct: 305 LSTLDLSTEHKVLDVKNRIEASIVIWKRKLHLKDNKSSWGSAVSLEKRELFEERAETILV 364

Query: 405 ILKHRFPGIPQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGL--- 464
           +LK +FPG+PQSSLDISKIQ+N+DVGHAVLESYSRILESL +T MSRI+DVL+AD L   
Sbjct: 365 LLKQKFPGLPQSSLDISKIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARK 424

Query: 465 ------TQNPSQIATRRKPTSEPPMEKS-EELNNNGPETPASMTLLDFMGWGQDQNEAEM 524
                 T +  +IAT     S      S EE+     +  +  TLLDF+GW  + ++ + 
Sbjct: 425 QCTGEETSDGGKIATETDSASAGSSNYSGEEIEKLESQNSSKTTLLDFIGWSDNSSKGQS 484

Query: 525 KKESFGNSDDLNADSDLKQGNKPGNIVTNKKVSY---LENLSAVRSPTARH 557
           +K                        +T KK+SY   LENL+  RSP  RH
Sbjct: 485 EKP------------------PKSPRMTPKKLSYLEKLENLNGFRSPKDRH 517

BLAST of PI0009268 vs. ExPASy Swiss-Prot
Match: F4J1G1 (Rop guanine nucleotide exchange factor 13 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF13 PE=1 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 9.6e-138
Identity = 292/598 (48.83%), Postives = 386/598 (64.55%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHE--NTGRHAKSLSVESA-NIFDTVIEDDLSTSRSQGS 60
           MV+A E+EHE ++ + F      +  ++ RH K  + +SA  I D  I+D     ++  S
Sbjct: 1   MVKASEKEHEKYKPKLFDLENVKKKNSSSRHFKRWNSDSALRIEDPDIDDGTVFKKTATS 60

Query: 61  TPVHNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPA-------------AEKEAKDK 120
           +              P+ P    +  P+P+ ++ ++                 EK  +D+
Sbjct: 61  S------------IQPILPVLAMDEQPQPREATDEESQKDSGKREIISFLGYNEKFTRDQ 120

Query: 121 M----------------AVEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAAS 180
           +                  E+EQMK++FAKLLLGEDMSGG KGVSSALALSNAITNLAAS
Sbjct: 121 VILRFRGLQAKSCYFAYVTELEQMKDKFAKLLLGEDMSGGSKGVSSALALSNAITNLAAS 180

Query: 181 VFGEQWRLEPMSAERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDL 240
            FGE  RLE +S ++K RWR+EI  LLSVTD+IVEF P+ Q ++DG++ME+M T+QR DL
Sbjct: 181 AFGEIRRLEAISEDKKERWRREIGWLLSVTDHIVEFSPTHQTNEDGSSMEVMTTKQRTDL 240

Query: 241 HLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDLEKGNNKRKDDKWWLPTAKVPPNG 300
             NIP+L+KLD ML+DCLD FKDQ+EFYYV+  + + E  N+ R DDKWWLP  KVPP G
Sbjct: 241 VSNIPSLKKLDEMLLDCLDKFKDQDEFYYVTPGSPESENSNSTRNDDKWWLPIVKVPPKG 300

Query: 301 LSDMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYKSI 360
           LS+  ++FL  Q++CV QVL +AMAIN+Q+++EMEIPE YI+SLPK GRASLGD IY+ I
Sbjct: 301 LSETLKRFLLSQRECVCQVLNSAMAINSQVLTEMEIPESYIDSLPKKGRASLGDMIYRMI 360

Query: 361 TVEFFDPDQFLSSMDLTSEHKILDLKNRIEASIVIWRRKMNQKDGKST--WGSAVSLEKR 420
           T+E FD +QFL  MDL+SEHKILDLKN+ EAS+VIW+RK+ Q D KS+  W + +S++KR
Sbjct: 361 TLEMFDAEQFLLEMDLSSEHKILDLKNKFEASVVIWQRKIVQIDNKSSSPWSTNLSMDKR 420

Query: 421 ELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVMSRI 480
           +  EERA TIL ++K  FPGI QS+LDISKIQFNRD+G A++ESYSRILESLA TVMSRI
Sbjct: 421 QQLEERAATILQLIKQEFPGISQSTLDISKIQFNRDIGLAIVESYSRILESLAHTVMSRI 480

Query: 481 EDVLHADGLTQNPSQIATR---RKPTSEPPMEKSEE-----LNNNGPETPASMTLLDFMG 540
           EDVL AD LTQNP     +    K T  P  EK EE     L +   +   +++L + M 
Sbjct: 481 EDVLEADQLTQNPELAMCKIHIVKETESP--EKEEEPNFCLLEDRPKKQKPTISLSEVMQ 540

Query: 541 WGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIV--TNKKVSYLENLSAVRSPTA 555
           W  + NE   +K            SD K   +  +++   NKK +YLE+L   RSPTA
Sbjct: 541 WNIETNEPRKEK------------SDKKLLTRVSSMIMSNNKKTTYLESLGTTRSPTA 572

BLAST of PI0009268 vs. ExPASy TrEMBL
Match: A0A5D3C0X6 (Rop guanine nucleotide exchange factor 12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002180 PE=4 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.4e-301
Identity = 537/556 (96.58%), Postives = 545/556 (98.02%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           MVRAMEQEHEGFRSRFF FRGTHENTGRHAKSLSVESAN+FD+  EDDLSTSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           HNNSSEKHQ  PPLAPSNRSERAPKPKLSSKDDD A EKEA++KMA+EMEQMKERFAKLL
Sbjct: 61  HNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADD EKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTSEHKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL 480
           RDVGHAVLESYSRILESLAFTVMSRIEDVLHAD LTQNPSQIATRRKPTSEPPMEKSEEL
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKSEEL 480

Query: 481 NNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKV 540
           NNNGPETPASMTLLDFMGWGQDQNE EMKKE FGNSDDLNADSDLKQGNKPGNIVTNKKV
Sbjct: 481 NNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNKKV 540

Query: 541 SYLENLSAVRSPTARH 557
           SYLENLSAVRSPTARH
Sbjct: 541 SYLENLSAVRSPTARH 556

BLAST of PI0009268 vs. ExPASy TrEMBL
Match: A0A1S3CGA0 (LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like OS=Cucumis melo OX=3656 GN=LOC103500592 PE=4 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.6e-300
Identity = 535/556 (96.22%), Postives = 544/556 (97.84%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           MVRAMEQEHEGFRSRFF FRGTHENTGRHAKSLSVESAN+FD+  EDDLSTSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           HNNSSEKHQ  PPLAPSNRSERAPKPKLSSKDDD A EKEA++KMA+EMEQMKERFAKLL
Sbjct: 61  HNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADD EKGNNK KDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKXKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTSEHKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL 480
           RDVGHAVLESYSRILESLAFTVMSRIEDVLHAD LTQNPSQIATRRKPTSEPPMEKSEEL
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKSEEL 480

Query: 481 NNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKV 540
           NNNGPETPASMTLLDFMGWGQDQNE EMKKE FGNSDDLNADSDLKQGNKPGNIVTNK+V
Sbjct: 481 NNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNKRV 540

Query: 541 SYLENLSAVRSPTARH 557
           SYLENLSAVRSPTARH
Sbjct: 541 SYLENLSAVRSPTARH 556

BLAST of PI0009268 vs. ExPASy TrEMBL
Match: A0A0A0K7D8 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G446980 PE=4 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.4e-293
Identity = 530/556 (95.32%), Postives = 540/556 (97.12%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           MVRAMEQEHEGFRSRFF FRG HENTGRHAKSLSVESAN+FD+V EDDLSTSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGNHENTGRHAKSLSVESANVFDSVNEDDLSTSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           H NSSEKHQ  PPLAPSNRSERA KPKLSSKD+D AAEKEA++KMA+EMEQMKERFAKLL
Sbjct: 61  H-NSSEKHQAGPPLAPSNRSERASKPKLSSKDNDLAAEKEAREKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DA+D EKGNNKR +DKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DANDSEKGNNKR-NDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKMNQKDGKSTWGSAVS+EKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSMEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL 480
           RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEK EEL
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKLEEL 480

Query: 481 NNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKV 540
            NNGPETPASMTLLDFMGWGQDQNE EMKKESFGNSDDLN DSDLKQGNK GNIVTNKKV
Sbjct: 481 -NNGPETPASMTLLDFMGWGQDQNETEMKKESFGNSDDLNLDSDLKQGNKAGNIVTNKKV 540

Query: 541 SYLENLSAVRSPTARH 557
           SYLENLSAVRSPTARH
Sbjct: 541 SYLENLSAVRSPTARH 553

BLAST of PI0009268 vs. ExPASy TrEMBL
Match: A0A6J1HL16 (rop guanine nucleotide exchange factor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111464520 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.3e-258
Identity = 468/557 (84.02%), Postives = 508/557 (91.20%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           M+ AMEQEHEGF+S+  HF+GTHEN+GRHAKSLS+ESA++ D+++++D  TSRSQGSTPV
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDSLVDED--TSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           HNNS+EK     PL P+NR E++ K KL +KD+D   EKE +DK A+EMEQMKERFAKLL
Sbjct: 61  HNNSAEK-----PLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERK RWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADD EKGNNKRKDDKWWLPTA+VPPNGLS+M+RKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTS+HKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKM  KD KS W SAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKS-EE 480
           RDVGHA+LESYSRILESLAFTVMSRIEDVLHAD LTQNPSQ A++RKP SE P EK+  +
Sbjct: 421 RDVGHAILESYSRILESLAFTVMSRIEDVLHADALTQNPSQTASKRKPASETPPEKTPRK 480

Query: 481 LNNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKK 540
             +NG ETP SMTLLDFMGWGQ+Q+E E KK S G SD+LNADSD K GN PGN+VTNKK
Sbjct: 481 REDNGTETPVSMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNVVTNKK 540

Query: 541 VSYLENLSAVRSPTARH 557
           VSY+ENLSAV+SPTARH
Sbjct: 541 VSYVENLSAVKSPTARH 547

BLAST of PI0009268 vs. ExPASy TrEMBL
Match: A0A6J1KI31 (rop guanine nucleotide exchange factor 12-like OS=Cucurbita maxima OX=3661 GN=LOC111494607 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.7e-257
Identity = 468/557 (84.02%), Postives = 505/557 (90.66%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           M+ AMEQEHEGF+S+  HF+GTHEN+GRHAKSLS+E A + D+++++D  TSRSQGSTPV
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDED--TSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           HNNS+EK     PL P+NR E++ K KL +KD+D   EKE +DK A+EMEQMKERFAKLL
Sbjct: 61  HNNSAEK-----PLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERK RWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADD EKGNNKRKDDKWWLPTA+VPPNGLS+MSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTS+HKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKM  KD KS W SAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSE-E 480
           RDVGHA+LESYSRILESLAFTVMSRIEDVLHAD +TQNPSQ A +RKP SE P EK+  +
Sbjct: 421 RDVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCK 480

Query: 481 LNNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKK 540
             +NG ETP SMTLLDFMGWGQ+Q+E E KK S G SD+LNADSD K GN PGNIVTNKK
Sbjct: 481 REDNGTETPVSMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKK 540

Query: 541 VSYLENLSAVRSPTARH 557
           VSY+ENLSAV+SPTARH
Sbjct: 541 VSYVENLSAVKSPTARH 547

BLAST of PI0009268 vs. NCBI nr
Match: KAA0059309.1 (rop guanine nucleotide exchange factor 12-like [Cucumis melo var. makuwa] >TYK04019.1 rop guanine nucleotide exchange factor 12-like [Cucumis melo var. makuwa])

HSP 1 Score: 1044.6 bits (2700), Expect = 2.9e-301
Identity = 537/556 (96.58%), Postives = 545/556 (98.02%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           MVRAMEQEHEGFRSRFF FRGTHENTGRHAKSLSVESAN+FD+  EDDLSTSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           HNNSSEKHQ  PPLAPSNRSERAPKPKLSSKDDD A EKEA++KMA+EMEQMKERFAKLL
Sbjct: 61  HNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADD EKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTSEHKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL 480
           RDVGHAVLESYSRILESLAFTVMSRIEDVLHAD LTQNPSQIATRRKPTSEPPMEKSEEL
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKSEEL 480

Query: 481 NNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKV 540
           NNNGPETPASMTLLDFMGWGQDQNE EMKKE FGNSDDLNADSDLKQGNKPGNIVTNKKV
Sbjct: 481 NNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNKKV 540

Query: 541 SYLENLSAVRSPTARH 557
           SYLENLSAVRSPTARH
Sbjct: 541 SYLENLSAVRSPTARH 556

BLAST of PI0009268 vs. NCBI nr
Match: XP_008462168.2 (PREDICTED: LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like [Cucumis melo])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.2e-300
Identity = 535/556 (96.22%), Postives = 544/556 (97.84%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           MVRAMEQEHEGFRSRFF FRGTHENTGRHAKSLSVESAN+FD+  EDDLSTSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           HNNSSEKHQ  PPLAPSNRSERAPKPKLSSKDDD A EKEA++KMA+EMEQMKERFAKLL
Sbjct: 61  HNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADD EKGNNK KDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKXKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTSEHKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL 480
           RDVGHAVLESYSRILESLAFTVMSRIEDVLHAD LTQNPSQIATRRKPTSEPPMEKSEEL
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKSEEL 480

Query: 481 NNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKV 540
           NNNGPETPASMTLLDFMGWGQDQNE EMKKE FGNSDDLNADSDLKQGNKPGNIVTNK+V
Sbjct: 481 NNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNKRV 540

Query: 541 SYLENLSAVRSPTARH 557
           SYLENLSAVRSPTARH
Sbjct: 541 SYLENLSAVRSPTARH 556

BLAST of PI0009268 vs. NCBI nr
Match: XP_038899805.1 (rop guanine nucleotide exchange factor 12-like [Benincasa hispida])

HSP 1 Score: 1028.1 bits (2657), Expect = 2.8e-296
Identity = 527/556 (94.78%), Postives = 541/556 (97.30%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           MVRAMEQE++GFRSRFFHFRGTHE+TGRHAKSLS+ESA++ ++VIEDDLSTSRSQGSTPV
Sbjct: 1   MVRAMEQENDGFRSRFFHFRGTHESTGRHAKSLSIESASMLESVIEDDLSTSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           HNNS+EK Q  PPLAPSNRSERAPKPK SSKDDDPA EKEAKDKMA+EMEQMKERFAKLL
Sbjct: 61  HNNSAEKQQAGPPLAPSNRSERAPKPKSSSKDDDPATEKEAKDKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADD EKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL 480
           RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRK TSE P+EKSEE+
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKSTSEAPVEKSEEM 480

Query: 481 NNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKV 540
           NNNGPETPASMTLLDFMGWG DQ+E E KK+SFGNSDDL ADSDLKQ NKPGNIVTNKKV
Sbjct: 481 NNNGPETPASMTLLDFMGWGADQSETETKKDSFGNSDDLYADSDLKQANKPGNIVTNKKV 540

Query: 541 SYLENLSAVRSPTARH 557
           SYLENLSAVRSPTARH
Sbjct: 541 SYLENLSAVRSPTARH 556

BLAST of PI0009268 vs. NCBI nr
Match: XP_011659571.1 (rop guanine nucleotide exchange factor 12 [Cucumis sativus])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 530/556 (95.32%), Postives = 540/556 (97.12%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           MVRAMEQEHEGFRSRFF FRG HENTGRHAKSLSVESAN+FD+V EDDLSTSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGNHENTGRHAKSLSVESANVFDSVNEDDLSTSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           H NSSEKHQ  PPLAPSNRSERA KPKLSSKD+D AAEKEA++KMA+EMEQMKERFAKLL
Sbjct: 61  H-NSSEKHQAGPPLAPSNRSERASKPKLSSKDNDLAAEKEAREKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DA+D EKGNNKR +DKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DANDSEKGNNKR-NDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKMNQKDGKSTWGSAVS+EKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSMEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL 480
           RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEK EEL
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKLEEL 480

Query: 481 NNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKV 540
            NNGPETPASMTLLDFMGWGQDQNE EMKKESFGNSDDLN DSDLKQGNK GNIVTNKKV
Sbjct: 481 -NNGPETPASMTLLDFMGWGQDQNETEMKKESFGNSDDLNLDSDLKQGNKAGNIVTNKKV 540

Query: 541 SYLENLSAVRSPTARH 557
           SYLENLSAVRSPTARH
Sbjct: 541 SYLENLSAVRSPTARH 553

BLAST of PI0009268 vs. NCBI nr
Match: KAG7025807.1 (Rop guanine nucleotide exchange factor 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 902.1 bits (2330), Expect = 2.3e-258
Identity = 470/557 (84.38%), Postives = 508/557 (91.20%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGSTPV 60
           M+ AMEQEHEGF+S+  HF+GTHEN+GRHAKSLS+ESA++ DT++++D  TSRSQGSTPV
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDTLVDED--TSRSQGSTPV 60

Query: 61  HNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLL 120
           HNNS+EK     PL P+NR E++ K KL +KD+D   EKE +DK A+EMEQMKERFAKLL
Sbjct: 61  HNNSAEK-----PLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERK RWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSR 240

Query: 241 DADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADD EKGNNKRKDDKWWLPTA+VPPNGLS+M+RKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTS+HKILDLK+RIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEAS 360

Query: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKM  KD KS W SAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKS-EE 480
           RDVGHA+LESYSRILESLAFTVMSRIEDVLHAD LTQNPSQ A++RKP SE P EK+  +
Sbjct: 421 RDVGHAILESYSRILESLAFTVMSRIEDVLHADALTQNPSQTASKRKPASETPPEKTPRK 480

Query: 481 LNNNGPETPASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKK 540
             +NG ETP SMTLLDFMGWGQ+Q+E E KK S G SD+LNADSD K GN PGNIVTNKK
Sbjct: 481 REDNGTETPVSMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNIVTNKK 540

Query: 541 VSYLENLSAVRSPTARH 557
           VSY+ENLSAV+SPTARH
Sbjct: 541 VSYVENLSAVKSPTARH 547

BLAST of PI0009268 vs. TAIR 10
Match: AT1G79860.1 (RHO guanyl-nucleotide exchange factor 12 )

HSP 1 Score: 612.5 bits (1578), Expect = 3.5e-175
Identity = 354/564 (62.77%), Postives = 414/564 (73.40%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFR---GTHENTGRHAKSLSVESANIFDTVIEDDLSTSRSQGS 60
           MVRA EQE E +RSR F+F+     + +  RH KSLSVE+      + E    +  ++  
Sbjct: 1   MVRASEQEQETYRSRLFNFKWRNNDNNSATRHNKSLSVETG-----LDEAATGSHDAEPL 60

Query: 61  TPVHNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFA 120
           T +H +        PPL+ S   E      L++     AA +  + ++  +MEQMKERF+
Sbjct: 61  TIIHPSQ------GPPLSRSAADEAV----LAAL----AASQARERQLLADMEQMKERFS 120

Query: 121 KLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSV 180
           KLLLGED SGGGKGVSSALALSNAITNLAASVFGEQ RLEPM AER+ARWRKEID LLSV
Sbjct: 121 KLLLGEDNSGGGKGVSSALALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLSV 180

Query: 181 TDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYY 240
           TDY+VEF PSQQK+KDGTNMEIM TRQR DLH+NIPAL+KLDAMLIDCL+NFKDQ+EF Y
Sbjct: 181 TDYVVEFAPSQQKNKDGTNMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFSY 240

Query: 241 VSRDADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQ 300
           +S+D+ DL+    KR D+KWW+PT KVPP+GLS+ SR+FLQYQKDCVNQVLKAAMAINAQ
Sbjct: 241 ISKDSPDLD---GKRNDEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINAQ 300

Query: 301 IISEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRI 360
           ++ EMEIPE YI+SLPKNGRASLGD +YK+ITV+FFDPDQFLSSMD++SEHKI+DLKNRI
Sbjct: 301 VLFEMEIPESYIDSLPKNGRASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNRI 360

Query: 361 EASIVIWRRKMNQKDGKST--WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDIS 420
           EASI+IW+RKM  KD KS+  W S VSLEKRE+FEERAETILLILK R+PGI QSSLDIS
Sbjct: 361 EASIIIWKRKMVYKDNKSSAPWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDIS 420

Query: 421 KIQFNRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPME 480
           KIQFN DVG AVLESYSRILESLA+TV+SRI+DVL AD           R       P+E
Sbjct: 421 KIQFNEDVGQAVLESYSRILESLAYTVLSRIDDVLEAD-----------RAGNKRNTPLE 480

Query: 481 KSEELNNNGPETPASMTLLDFMGWGQDQ---NEAEMKKESFGNSDDLNADSDLKQGNKPG 540
             EE          SMTL DFMGW  DQ    E E KK       DL  D  +K+     
Sbjct: 481 AEEE------TLVGSMTLSDFMGWDFDQAANAELESKK-------DLPDDPLIKE---KL 515

Query: 541 NIVTNKKVSYLENLSAVRSPTARH 557
           ++VT KK SYLE L  V+SPTARH
Sbjct: 541 SVVTTKKTSYLETLGGVKSPTARH 515

BLAST of PI0009268 vs. TAIR 10
Match: AT1G52240.1 (RHO guanyl-nucleotide exchange factor 11 )

HSP 1 Score: 579.3 bits (1492), Expect = 3.2e-165
Identity = 334/542 (61.62%), Postives = 389/542 (71.77%), Query Frame = 0

Query: 17  FHFRGTHENTGRHAKSLSVESANIFD--TVIEDDLSTSRSQGSTPVHNNSSEKHQVAPPL 76
           F+      +  RH KS S + A   D    ++DD +      S P    S ++  +    
Sbjct: 82  FNDNMNENSASRHVKSWSSDCAMRMDGSDNLDDDDNDMMMFRSQPGKCGSVDRPSLPIGG 141

Query: 77  APSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLLLGEDMSGGGKGVSS 136
              NR+++   P++SS D       EA   +   MEQMKE+F+KLLLGEDMSGGGKGVSS
Sbjct: 142 VTPNRNDKL--PRVSSSD-----SMEALIILQAAMEQMKEKFSKLLLGEDMSGGGKGVSS 201

Query: 137 ALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDG 196
           ALALSNAITNLAAS FGEQ RLEPM+ +RK RWR+EI  L+SV DYIVEF P+QQ +KDG
Sbjct: 202 ALALSNAITNLAASAFGEQRRLEPMAVDRKTRWRREIGWLISVADYIVEFAPTQQTNKDG 261

Query: 197 TNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDLEKGNNKRKD 256
           T+ME+M TRQR DL  NIPAL+KLDAML+DCLD FKDQ+EFYYV +D+ D       R D
Sbjct: 262 TSMEVMSTRQRTDLLCNIPALKKLDAMLLDCLDKFKDQDEFYYVKKDSPD---SCETRND 321

Query: 257 DKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPK 316
           +KWWLP  KVPPNGLS++SR+FLQ QK+CVNQVLKAAMAINAQ++SEMEIPE Y+ESLPK
Sbjct: 322 EKWWLPAVKVPPNGLSEISRRFLQSQKECVNQVLKAAMAINAQVLSEMEIPESYLESLPK 381

Query: 317 NGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEASIVIWRRKMNQKDGK 376
           NGRASLGD IY+ ITVE FD DQFL  MDL+SEHKILDLKNRIEASIVIW+RKM QKD K
Sbjct: 382 NGRASLGDVIYRMITVEMFDADQFLIEMDLSSEHKILDLKNRIEASIVIWKRKMVQKDTK 441

Query: 377 STWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAVLESYSRI 436
           S WGS VS+EKRE FEERAETILL+LK  FPGI QSSLDISKIQFNRDVG A+LESYSR+
Sbjct: 442 SPWGSTVSIEKREQFEERAETILLLLKQGFPGISQSSLDISKIQFNRDVGLAILESYSRV 501

Query: 437 LESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEELNNNGPETPASMTLL 496
           LESLA TVMSRIEDVL+AD LTQ P+  A  +   S    EK  E   +  E  AS TL 
Sbjct: 502 LESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSLKENEKLREERLSFTEDMASGTLS 561

Query: 497 DFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIVTNKKVSYLENLSAVRSPTA 556
           D M WG   N  EMKKESF    +    S +      G +  NKK SYL+NL A+RSPTA
Sbjct: 562 DVMQWGNKNN--EMKKESFFGDREKPLLSKV-----TGIMTNNKKSSYLDNLGAMRSPTA 606

BLAST of PI0009268 vs. TAIR 10
Match: AT3G24620.1 (RHO guanyl-nucleotide exchange factor 8 )

HSP 1 Score: 559.7 bits (1441), Expect = 2.7e-159
Identity = 317/555 (57.12%), Postives = 387/555 (69.73%), Query Frame = 0

Query: 14  SRFFHFRGTHENTG-RHAKSLSVESANIFDTVIEDDLSTSRSQGSTPVHNNSSEKHQVAP 73
           S+ F+F+   +++  +  +S ++   N    ++E +   S++  S       S    ++P
Sbjct: 12  SKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFVESPVESSLPMISP 71

Query: 74  PLAPSNRSERAPKPKLSSKDDDPAAEKEAKDKMAVEMEQMKERFAKLLLGEDMSGGGKGV 133
              P  RSER                         +ME MK+RFAKLLLGEDMSGGGKGV
Sbjct: 72  LTRPGKRSER----------------------QQADMEMMKDRFAKLLLGEDMSGGGKGV 131

Query: 134 SSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDYIVEFVPSQQKSK 193
           SSALALSNAITNLAAS+FGEQ +L+PM  +R+ARW+KEID LLSVTD+IVEFVPSQQ SK
Sbjct: 132 SSALALSNAITNLAASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSK 191

Query: 194 DGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDLEKGNNKR 253
           DG   EIMVTRQR DL +NIPALRKLDAMLID LDNF+  NEF+YVSRD+++ ++  N R
Sbjct: 192 DGVCTEIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDR 251

Query: 254 KDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESL 313
            +DKWWLP  KVPP GLS+ SR+ L +QKD V QV KAAMAINAQ++SEMEIPE YI+SL
Sbjct: 252 TNDKWWLPPVKVPPGGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSL 311

Query: 314 PKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEASIVIWRRKMNQKD 373
           PKNGRASLGDSIYKSIT E+FDP+QFL+ +D+++EHK+LDLKNRIEAS+VIW+RK++ KD
Sbjct: 312 PKNGRASLGDSIYKSITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKD 371

Query: 374 GKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAVLESYS 433
            KS+WGSAVSLEKRELFEERAETIL++LK +FPG+PQSSLDISKIQFN+DVG AVLESYS
Sbjct: 372 TKSSWGSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYS 431

Query: 434 RILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKSEEL--NNNGPET--- 493
           RILESLA+TVMSRIEDVL+ D L    + +A             SE    +N+G E    
Sbjct: 432 RILESLAYTVMSRIEDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKH 491

Query: 494 -PASMTLLDFMGWGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNI--VTNKKVSY-- 553
            P S TLLDFMGW                      D+  K G+KP     +T KK+SY  
Sbjct: 492 DPHSKTLLDFMGWN---------------------DNSSKGGDKPTKSPNLTPKKLSYLE 523

Query: 554 -LENLSAVRSPTARH 557
            LENL+  RSP  RH
Sbjct: 552 KLENLNGFRSPKDRH 523

BLAST of PI0009268 vs. TAIR 10
Match: AT4G13240.1 (RHO guanyl-nucleotide exchange factor 9 )

HSP 1 Score: 548.1 bits (1411), Expect = 8.0e-156
Identity = 305/531 (57.44%), Postives = 380/531 (71.56%), Query Frame = 0

Query: 45  IEDDLSTSRSQGSTPVHNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPAAEKEAK-- 104
           +E  +S + S     + ++S  K Q  P LA +   E   +  L     + +    ++  
Sbjct: 5   LERGISITSSFNLDRMFDSSPGKEQQQPHLAETTMPESQTQDSLGGSPVETSRPMTSRLI 64

Query: 105 ----DKMAVEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEP 164
               DK   E E MK+RF KLLLGEDMSGGGKGVSSALALSNAITNLAAS+FGEQ +L+P
Sbjct: 65  SRRQDKQQSETEMMKDRFTKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQP 124

Query: 165 MSAERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKL 224
           M+ +R+ARW+KEID LLSVTD+IVEFVPSQQ SK+G   EIMVTRQR DL +NIPALRKL
Sbjct: 125 MAPDRRARWKKEIDWLLSVTDHIVEFVPSQQISKEGVCTEIMVTRQRGDLLMNIPALRKL 184

Query: 225 DAMLIDCLDNFKDQNEFYYVSRDADDLEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQ 284
           DAMLID LDNF+  NEF+YVSRD+++ ++  N+R  DKWWLP  KVPPNGLS+ +R+ L 
Sbjct: 185 DAMLIDTLDNFRGHNEFWYVSRDSEEGKQARNERTKDKWWLPPVKVPPNGLSESARRMLH 244

Query: 285 YQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQF 344
           +QKD V+QV KAAMAINAQ++SEM IP+ YIESLPKNGR SLGDS+YKSIT E+FDP+QF
Sbjct: 245 FQKDSVSQVQKAAMAINAQVLSEMAIPDSYIESLPKNGRVSLGDSLYKSITEEWFDPEQF 304

Query: 345 LSSMDLTSEHKILDLKNRIEASIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILL 404
           LS++DL++EHK+LD+KNRIEASIVIW+RK++ KD KS+WGSAVSLEKRELFEERAETIL+
Sbjct: 305 LSTLDLSTEHKVLDVKNRIEASIVIWKRKLHLKDNKSSWGSAVSLEKRELFEERAETILV 364

Query: 405 ILKHRFPGIPQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGL--- 464
           +LK +FPG+PQSSLDISKIQ+N+DVGHAVLESYSRILESL +T MSRI+DVL+AD L   
Sbjct: 365 LLKQKFPGLPQSSLDISKIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARK 424

Query: 465 ------TQNPSQIATRRKPTSEPPMEKS-EELNNNGPETPASMTLLDFMGWGQDQNEAEM 524
                 T +  +IAT     S      S EE+     +  +  TLLDF+GW  + ++ + 
Sbjct: 425 QCTGEETSDGGKIATETDSASAGSSNYSGEEIEKLESQNSSKTTLLDFIGWSDNSSKGQS 484

Query: 525 KKESFGNSDDLNADSDLKQGNKPGNIVTNKKVSY---LENLSAVRSPTARH 557
           +K                        +T KK+SY   LENL+  RSP  RH
Sbjct: 485 EKP------------------PKSPRMTPKKLSYLEKLENLNGFRSPKDRH 517

BLAST of PI0009268 vs. TAIR 10
Match: AT3G16130.1 (RHO guanyl-nucleotide exchange factor 13 )

HSP 1 Score: 491.9 bits (1265), Expect = 6.8e-139
Identity = 292/598 (48.83%), Postives = 386/598 (64.55%), Query Frame = 0

Query: 1   MVRAMEQEHEGFRSRFFHFRGTHE--NTGRHAKSLSVESA-NIFDTVIEDDLSTSRSQGS 60
           MV+A E+EHE ++ + F      +  ++ RH K  + +SA  I D  I+D     ++  S
Sbjct: 1   MVKASEKEHEKYKPKLFDLENVKKKNSSSRHFKRWNSDSALRIEDPDIDDGTVFKKTATS 60

Query: 61  TPVHNNSSEKHQVAPPLAPSNRSERAPKPKLSSKDDDPA-------------AEKEAKDK 120
           +              P+ P    +  P+P+ ++ ++                 EK  +D+
Sbjct: 61  S------------IQPILPVLAMDEQPQPREATDEESQKDSGKREIISFLGYNEKFTRDQ 120

Query: 121 M----------------AVEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAAS 180
           +                  E+EQMK++FAKLLLGEDMSGG KGVSSALALSNAITNLAAS
Sbjct: 121 VILRFRGLQAKSCYFAYVTELEQMKDKFAKLLLGEDMSGGSKGVSSALALSNAITNLAAS 180

Query: 181 VFGEQWRLEPMSAERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDL 240
            FGE  RLE +S ++K RWR+EI  LLSVTD+IVEF P+ Q ++DG++ME+M T+QR DL
Sbjct: 181 AFGEIRRLEAISEDKKERWRREIGWLLSVTDHIVEFSPTHQTNEDGSSMEVMTTKQRTDL 240

Query: 241 HLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDLEKGNNKRKDDKWWLPTAKVPPNG 300
             NIP+L+KLD ML+DCLD FKDQ+EFYYV+  + + E  N+ R DDKWWLP  KVPP G
Sbjct: 241 VSNIPSLKKLDEMLLDCLDKFKDQDEFYYVTPGSPESENSNSTRNDDKWWLPIVKVPPKG 300

Query: 301 LSDMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYKSI 360
           LS+  ++FL  Q++CV QVL +AMAIN+Q+++EMEIPE YI+SLPK GRASLGD IY+ I
Sbjct: 301 LSETLKRFLLSQRECVCQVLNSAMAINSQVLTEMEIPESYIDSLPKKGRASLGDMIYRMI 360

Query: 361 TVEFFDPDQFLSSMDLTSEHKILDLKNRIEASIVIWRRKMNQKDGKST--WGSAVSLEKR 420
           T+E FD +QFL  MDL+SEHKILDLKN+ EAS+VIW+RK+ Q D KS+  W + +S++KR
Sbjct: 361 TLEMFDAEQFLLEMDLSSEHKILDLKNKFEASVVIWQRKIVQIDNKSSSPWSTNLSMDKR 420

Query: 421 ELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAVLESYSRILESLAFTVMSRI 480
           +  EERA TIL ++K  FPGI QS+LDISKIQFNRD+G A++ESYSRILESLA TVMSRI
Sbjct: 421 QQLEERAATILQLIKQEFPGISQSTLDISKIQFNRDIGLAIVESYSRILESLAHTVMSRI 480

Query: 481 EDVLHADGLTQNPSQIATR---RKPTSEPPMEKSEE-----LNNNGPETPASMTLLDFMG 540
           EDVL AD LTQNP     +    K T  P  EK EE     L +   +   +++L + M 
Sbjct: 481 EDVLEADQLTQNPELAMCKIHIVKETESP--EKEEEPNFCLLEDRPKKQKPTISLSEVMQ 540

Query: 541 WGQDQNEAEMKKESFGNSDDLNADSDLKQGNKPGNIV--TNKKVSYLENLSAVRSPTA 555
           W  + NE   +K            SD K   +  +++   NKK +YLE+L   RSPTA
Sbjct: 541 WNIETNEPRKEK------------SDKKLLTRVSSMIMSNNKKTTYLESLGTTRSPTA 572

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9CA894.9e-17462.77Rop guanine nucleotide exchange factor 12 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Q9M8114.6e-17262.25Rop guanine nucleotide exchange factor 11 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Q9LV403.7e-15857.12Rho guanine nucleotide exchange factor 8 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9SVQ31.1e-15457.44Rop guanine nucleotide exchange factor 9 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
F4J1G19.6e-13848.83Rop guanine nucleotide exchange factor 13 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Match NameE-valueIdentityDescription
A0A5D3C0X61.4e-30196.58Rop guanine nucleotide exchange factor 12-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CGA01.6e-30096.22LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like OS=Cucumis m... [more]
A0A0A0K7D85.4e-29395.32PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G446980 PE=4 ... [more]
A0A6J1HL164.3e-25884.02rop guanine nucleotide exchange factor 12-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KI313.7e-25784.02rop guanine nucleotide exchange factor 12-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
KAA0059309.12.9e-30196.58rop guanine nucleotide exchange factor 12-like [Cucumis melo var. makuwa] >TYK04... [more]
XP_008462168.23.2e-30096.22PREDICTED: LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like [... [more]
XP_038899805.12.8e-29694.78rop guanine nucleotide exchange factor 12-like [Benincasa hispida][more]
XP_011659571.11.1e-29295.32rop guanine nucleotide exchange factor 12 [Cucumis sativus][more]
KAG7025807.12.3e-25884.38Rop guanine nucleotide exchange factor 12 [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
AT1G79860.13.5e-17562.77RHO guanyl-nucleotide exchange factor 12 [more]
AT1G52240.13.2e-16561.62RHO guanyl-nucleotide exchange factor 11 [more]
AT3G24620.12.7e-15957.12RHO guanyl-nucleotide exchange factor 8 [more]
AT4G13240.18.0e-15657.44RHO guanyl-nucleotide exchange factor 9 [more]
AT3G16130.16.8e-13948.83RHO guanyl-nucleotide exchange factor 13 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005512PRONE domainPFAMPF03759PRONEcoord: 108..457
e-value: 1.0E-175
score: 583.9
IPR005512PRONE domainPROSITEPS51334PRONEcoord: 99..463
score: 116.631088
NoneNo IPR availableGENE3D1.20.58.2010PRONE domain, subdomain 1coord: 113..454
e-value: 7.7E-152
score: 506.8
NoneNo IPR availableGENE3D1.20.58.1310PRONE domain, subdomain 2coord: 303..419
e-value: 7.7E-152
score: 506.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 457..491
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 509..533
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..102
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 475..489
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 518..533
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..102
NoneNo IPR availablePANTHERPTHR33101:SF10ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 12coord: 13..556
IPR038937Rop guanine nucleotide exchange factorPANTHERPTHR33101ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 13..556

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009268.1PI0009268.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity