PI0009169 (gene) Melon (PI 482460) v1

Overview
NamePI0009169
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr10: 19858890 .. 19860155 (+)
RNA-Seq ExpressionPI0009169
SyntenyPI0009169
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGAGAGCAATGACGACAAAGCCAATAATGTCTTCAGCTGAAGTTATCTTAGCTTCCATCTCAGAAATCATTGATTCCGCAGCGTGCACTGAAGAAGAACATGGAGAATTTATTGAGATCGGAAGCTACTTTTATCGAGTAGCTCTGGCTGTTGGGGAGTTGCAGGCAATAGACCCCATAAGATTTGATGAAATCCTCCAATCTCTAAACAAAAGCATCAACCTTGCGAAGTATCTTCTGGACAAGTTCCTAACCAGCATTCAACCAGTCTCCAATTCTGATCCCGTTAGCATCATAAATCCATTAGAAGAGGTGATAAAAGAGATGGGTGAATGCTTGAACAAGATAGCAACCTTTACATTCGAGGAACAAAATTATGTGAAAGTGGCAATTTTATCACTTTCAGATGAGATGAAGAATATAAACACTAAAATTGACCAAGCTCAAGCCAATACGAACATGCAAGAGATTCAAACTTCTTTGGAGGAACAGTCAGAAAAAGTACCAGAAGTTATAGAAAAAGATCTATACCCCATTGACATGGACTGGGACACAACCAATACACAATCTCCAGTTGCATCAGAGTTGAGTGACGCTGTTAATATTAGAAAGGGTGGAAGAAGCCAAATGAAGTATAGAAATGTTACATCAGACGTGGAAAAACTACCCTCAATGACCCATTACACAGAACCTCTCTTTGAGACCTTCATCTGTCCATTGACAAAGAATATAATGAAAGATCCAGTTACCTTAGAAACGGGAGTGTCATATGAAAGACAAGCAATTCTTGAGTGGTTTCAAGAGTTTAAAGAATTTGAGGAAATTTTCTGCCCAGTCACAGGGCAAAAGCTTGTGTCCAAAGCTTTTAATTCCAATAGAGCTCTGAAGTCTACAATAGACAAATGGAATGAACGCAATGAGGCAGCGACAATTGAAGTCACCCGGGATGCTCTATCTTTAGCCAGTTCAGATGAAATGGTGCTTGAGGCAATCAAAGACCTGTCGAGCATCAGCAAAGGGAAGCAGTTCAACATAGAACAGATCTTCAATTTTGACATGTTACCGTTGCTTGTTAACTTTCTGGAGTACAGAGACAGAGATGTTAGATATGCAGTTCTACAGCTATTGCATCAAATGGTAGAAGTCAATGAAGACAACAAGGTTCAAATTCATTCTAGTTGTCTTATTATTGTACACAATTCATTGCTGTCTGTCATGTCTCTGCAGTTCTTCCCTCTTCCCATCCCCCCACCCCAAATATAA

mRNA sequence

ATGTTGAGAGCAATGACGACAAAGCCAATAATGTCTTCAGCTGAAGTTATCTTAGCTTCCATCTCAGAAATCATTGATTCCGCAGCGTGCACTGAAGAAGAACATGGAGAATTTATTGAGATCGGAAGCTACTTTTATCGAGTAGCTCTGGCTGTTGGGGAGTTGCAGGCAATAGACCCCATAAGATTTGATGAAATCCTCCAATCTCTAAACAAAAGCATCAACCTTGCGAAGTATCTTCTGGACAAGTTCCTAACCAGCATTCAACCAGTCTCCAATTCTGATCCCGTTAGCATCATAAATCCATTAGAAGAGGTGATAAAAGAGATGGGTGAATGCTTGAACAAGATAGCAACCTTTACATTCGAGGAACAAAATTATGTGAAAGTGGCAATTTTATCACTTTCAGATGAGATGAAGAATATAAACACTAAAATTGACCAAGCTCAAGCCAATACGAACATGCAAGAGATTCAAACTTCTTTGGAGGAACAGTCAGAAAAAGTACCAGAAGTTATAGAAAAAGATCTATACCCCATTGACATGGACTGGGACACAACCAATACACAATCTCCAGTTGCATCAGAGTTGAGTGACGCTGTTAATATTAGAAAGGGTGGAAGAAGCCAAATGAAGTATAGAAATGTTACATCAGACGTGGAAAAACTACCCTCAATGACCCATTACACAGAACCTCTCTTTGAGACCTTCATCTGTCCATTGACAAAGAATATAATGAAAGATCCAGTTACCTTAGAAACGGGAGTGTCATATGAAAGACAAGCAATTCTTGAGTGGTTTCAAGAGTTTAAAGAATTTGAGGAAATTTTCTGCCCAGTCACAGGGCAAAAGCTTGTGTCCAAAGCTTTTAATTCCAATAGAGCTCTGAAGTCTACAATAGACAAATGGAATGAACGCAATGAGGCAGCGACAATTGAAGTCACCCGGGATGCTCTATCTTTAGCCAGTTCAGATGAAATGGTGCTTGAGGCAATCAAAGACCTGTCGAGCATCAGCAAAGGGAAGCAGTTCAACATAGAACAGATCTTCAATTTTGACATGTTACCGTTGCTTGTTAACTTTCTGGAGTACAGAGACAGAGATGTTAGATATGCAGTTCTACAGCTATTGCATCAAATGGTAGAAGTCAATGAAGACAACAAGGTTCAAATTCATTCTAGTTGTCTTATTATTGTACACAATTCATTGCTGTCTGTCATGTCTCTGCAGTTCTTCCCTCTTCCCATCCCCCCACCCCAAATATAA

Coding sequence (CDS)

ATGTTGAGAGCAATGACGACAAAGCCAATAATGTCTTCAGCTGAAGTTATCTTAGCTTCCATCTCAGAAATCATTGATTCCGCAGCGTGCACTGAAGAAGAACATGGAGAATTTATTGAGATCGGAAGCTACTTTTATCGAGTAGCTCTGGCTGTTGGGGAGTTGCAGGCAATAGACCCCATAAGATTTGATGAAATCCTCCAATCTCTAAACAAAAGCATCAACCTTGCGAAGTATCTTCTGGACAAGTTCCTAACCAGCATTCAACCAGTCTCCAATTCTGATCCCGTTAGCATCATAAATCCATTAGAAGAGGTGATAAAAGAGATGGGTGAATGCTTGAACAAGATAGCAACCTTTACATTCGAGGAACAAAATTATGTGAAAGTGGCAATTTTATCACTTTCAGATGAGATGAAGAATATAAACACTAAAATTGACCAAGCTCAAGCCAATACGAACATGCAAGAGATTCAAACTTCTTTGGAGGAACAGTCAGAAAAAGTACCAGAAGTTATAGAAAAAGATCTATACCCCATTGACATGGACTGGGACACAACCAATACACAATCTCCAGTTGCATCAGAGTTGAGTGACGCTGTTAATATTAGAAAGGGTGGAAGAAGCCAAATGAAGTATAGAAATGTTACATCAGACGTGGAAAAACTACCCTCAATGACCCATTACACAGAACCTCTCTTTGAGACCTTCATCTGTCCATTGACAAAGAATATAATGAAAGATCCAGTTACCTTAGAAACGGGAGTGTCATATGAAAGACAAGCAATTCTTGAGTGGTTTCAAGAGTTTAAAGAATTTGAGGAAATTTTCTGCCCAGTCACAGGGCAAAAGCTTGTGTCCAAAGCTTTTAATTCCAATAGAGCTCTGAAGTCTACAATAGACAAATGGAATGAACGCAATGAGGCAGCGACAATTGAAGTCACCCGGGATGCTCTATCTTTAGCCAGTTCAGATGAAATGGTGCTTGAGGCAATCAAAGACCTGTCGAGCATCAGCAAAGGGAAGCAGTTCAACATAGAACAGATCTTCAATTTTGACATGTTACCGTTGCTTGTTAACTTTCTGGAGTACAGAGACAGAGATGTTAGATATGCAGTTCTACAGCTATTGCATCAAATGGTAGAAGTCAATGAAGACAACAAGGTTCAAATTCATTCTAGTTGTCTTATTATTGTACACAATTCATTGCTGTCTGTCATGTCTCTGCAGTTCTTCCCTCTTCCCATCCCCCCACCCCAAATATAA

Protein sequence

MLRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDPIRFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFTFEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPIDMDWDTTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPLTKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTIDKWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNFLEYRDRDVRYAVLQLLHQMVEVNEDNKVQIHSSCLIIVHNSLLSVMSLQFFPLPIPPPQI
Homology
BLAST of PI0009169 vs. ExPASy Swiss-Prot
Match: Q9CAA7 (Putative U-box domain-containing protein 42 OS=Arabidopsis thaliana OX=3702 GN=PUB42 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.1e-50
Identity = 134/401 (33.42%), Postives = 204/401 (50.87%), Query Frame = 0

Query: 65  EILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFTFEE 124
           +I +SL+ S+++AK L++K   S +  S +D  SI    E V+K+MGE L  I   TF+E
Sbjct: 15  DIFESLSDSVDVAKKLVEKSQESNEAESTTDLRSIEAGFEGVVKQMGETLQSIPESTFDE 74

Query: 125 QNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPIDMDW 184
           + Y+ V I SLS+EM+N            N  + + S +   + V E +E+DLYP D ++
Sbjct: 75  EEYIGVVIQSLSNEMQNATIGDGSKSEMINNGQQKISAKHTPDIVSEQMEEDLYPTDPEF 134

Query: 185 ----------------------------------------------------DTTNTQS- 244
                                                                +TN  S 
Sbjct: 135 SYESYMMYSESQSQMTDIPDIPSKSTDVSRQRKHGNHSESQSLVTEIPDIPSQSTNVSSQ 194

Query: 245 ----------PVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPL 304
                       ++E+ D  +      SQ KY N++  +  LP +T + EP ++ FICPL
Sbjct: 195 RKHGNLSKSQSQSTEIPDIPSQSSNASSQRKYGNLSESLSMLPQVTQFMEPPYQAFICPL 254

Query: 305 TKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTID 364
           TK IM+DPVT ETGV+ ERQA++EWF  F   +EI CPVTGQKL ++  ++N  LK+ I 
Sbjct: 255 TKEIMEDPVTTETGVTCERQAVIEWFDSFGNSDEINCPVTGQKLTTE-LSANVVLKTIIQ 314

Query: 365 KWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNF 398
           +W  RNEAA I+V   ALSL  S+ MV++A++DL    +GK++N  Q+    ++ LL  +
Sbjct: 315 EWKVRNEAARIKVAHAALSLGGSESMVIDALRDLQMTCEGKEYNKVQVREAGIIQLLDRY 374

BLAST of PI0009169 vs. ExPASy Swiss-Prot
Match: Q9LM76 (U-box domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN=PUB44 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 8.3e-25
Identity = 59/176 (33.52%), Postives = 111/176 (63.07%), Query Frame = 0

Query: 217 TSDVEKLPSMTHY---TEPLFETFICPLTKNIMKDPVTLETGVSYERQAILEWFQEFKEF 276
           +SD ++    +H+    + ++E FICPLTK +M DPVTLE G ++ER+AI +WF+E ++ 
Sbjct: 4   SSDGDQSDDSSHFERGVDHIYEAFICPLTKEVMHDPVTLENGRTFEREAIEKWFKECRDS 63

Query: 277 -EEIFCPVTGQKLVSKAFNSNRALKSTIDKWNERNEAATIEVTRDALSLASSDEMVLEAI 336
                CP+T Q+L S   +++ AL++TI++W  RN+AA +++ R +L L +++  +L+A+
Sbjct: 64  GRPPSCPLTSQELTSTDVSASIALRNTIEEWRSRNDAAKLDIARQSLFLGNAETDILQAL 123

Query: 337 KDLSSISKGKQFNIEQIFNFDMLPLLVNFLEYRDRDVRYAVLQLLHQMVEVNEDNK 389
             +  I +  + N   + N  ++ ++++ L+     VRY  LQ L  +VE ++++K
Sbjct: 124 MHVRQICRTIRSNRHGVRNSQLIHMIIDMLKSTSHRVRYKALQTLQVVVEGDDESK 179

BLAST of PI0009169 vs. ExPASy Swiss-Prot
Match: Q9SFX2 (U-box domain-containing protein 43 OS=Arabidopsis thaliana OX=3702 GN=PUB43 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 7.8e-23
Identity = 55/157 (35.03%), Postives = 102/157 (64.97%), Query Frame = 0

Query: 233 LFETFICPLTKNIMKDPVTLETGVSYERQAILEWFQEFKE-FEEIFCPVTGQKLVSKAFN 292
           ++E FICPLTK +M +PVTLE G ++ER+AI +WFQE +E  + + CP+T ++L     +
Sbjct: 25  IYEAFICPLTKQVMHNPVTLENGQTFEREAIEKWFQECRENGQPLSCPITSKELSITDLS 84

Query: 293 SNRALKSTIDKWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFN 352
            + AL++TI++W  RN+A  +++ R +L L +++  +L A+K++  I +  +   +++ N
Sbjct: 85  PSIALRNTIEEWRARNDALKLDIARQSLYLGNAETNILLALKNVREICRNIRKIRQRVCN 144

Query: 353 FDMLPLLVNFLEYRDRDVRYAVLQLLHQMVEVNEDNK 389
             ++ L+ + L+    +VR   LQ L  +VE +E++K
Sbjct: 145 PQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDEESK 181

BLAST of PI0009169 vs. ExPASy Swiss-Prot
Match: Q10FT0 (U-box domain-containing protein 24 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 7.5e-18
Identity = 58/172 (33.72%), Postives = 94/172 (54.65%), Query Frame = 0

Query: 231 EPLFETFICPLTKNIMKDPVTLETGVSYERQAILEWFQEFKE-FEEIFCPVTGQKLVSKA 290
           E  FE F+CPLTK +M+DPVT+ETG ++ER+AIL+WF+E ++      CP+T ++L    
Sbjct: 12  EGAFEAFVCPLTKQVMRDPVTIETGQTFEREAILKWFRECRDNGRRPTCPLTQRELRDTE 71

Query: 291 FNSNRALKSTIDKWNERNEAATIEVTRDALSLA----------SSDEMVLEAIKDLSSIS 350
            + + AL+S I +W  RNE   ++  R   SL             +E  L A+  +S I 
Sbjct: 72  VSPSVALRSVIHEWRARNEEKDLD--RACASLVGGFAGHAGDEEEEESALRALVHVSQIC 131

Query: 351 KGKQFNIEQIFNFDMLPLLVNFLEYRDRDVRYAVLQLLHQMVEVNEDNKVQI 392
           +    + + +    +L  +   L+   R +R   LQ+L  +VE N+DNK ++
Sbjct: 132 QRSAASKDLVRRRGVLRAVAEMLKSGSRRLRLKSLQVLRVLVEDNDDNKEEL 181

BLAST of PI0009169 vs. ExPASy Swiss-Prot
Match: Q681N2 (U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 1.3e-09
Identity = 51/169 (30.18%), Postives = 84/169 (49.70%), Query Frame = 0

Query: 237 FICPLTKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRAL 296
           F+CP+T  IM DPV + TG +YE+++I +WF    +     CP T Q+L   +   N AL
Sbjct: 294 FLCPITLEIMLDPVIIATGQTYEKESIQKWFDAGHK----TCPKTRQELDHLSLAPNFAL 353

Query: 297 KSTIDKWNERN----------------EAATIEVTRDALSLASSDEMVLEAIKDLSSISK 356
           K+ I +W E+N                +   + +  +ALS +  +E    ++K +  +++
Sbjct: 354 KNLIMQWCEKNNFKIPEKEVSPDSQNEQKDEVSLLVEALSSSQLEEQ-RRSVKQMRLLAR 413

Query: 357 GKQFNIEQIFNFDMLPLLVNFLEYRDRDVR-YAVLQLLHQMVEVNEDNK 389
               N   I N   +PLLV  L Y D  ++  AV  LL+  + ++E NK
Sbjct: 414 ENPENRVLIANAGAIPLLVQLLSYPDSGIQENAVTTLLN--LSIDEVNK 455

BLAST of PI0009169 vs. ExPASy TrEMBL
Match: A0A0A0KT18 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G607430 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 9.1e-192
Identity = 361/410 (88.05%), Postives = 380/410 (92.68%), Query Frame = 0

Query: 1   MLRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDP 60
           MLRAMTTKP+MSSAEV+LASISEIIDS ACTEEEHGEFIEIGSYFYR ALAV ELQA+DP
Sbjct: 1   MLRAMTTKPMMSSAEVVLASISEIIDSTACTEEEHGEFIEIGSYFYRAALAVEELQALDP 60

Query: 61  IRFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATF 120
            +FDEILQSLNKSINLAKYLL+KFLTSIQ VSNSDP+SIINPLEEVIK+MGECLNKIAT 
Sbjct: 61  TKFDEILQSLNKSINLAKYLLEKFLTSIQLVSNSDPISIINPLEEVIKQMGECLNKIATA 120

Query: 121 TFEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPI 180
           TF+EQNYVKVAILSLSDEMKNI+ KIDQAQA  NMQEIQTSLEEQSEKVPEVIEKDLYPI
Sbjct: 121 TFKEQNYVKVAILSLSDEMKNISIKIDQAQAIMNMQEIQTSLEEQSEKVPEVIEKDLYPI 180

Query: 181 DMDWD--TTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFI 240
           DMDWD  TTNTQSPVA ELS+AV IRKGGRSQMKYRN TSDVEKLPS THYT+PLFETFI
Sbjct: 181 DMDWDTSTTNTQSPVALELSNAVIIRKGGRSQMKYRNATSDVEKLPSKTHYTKPLFETFI 240

Query: 241 CPLTKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKS 300
           CPLTKNIM+DPVTLETGVSYERQAI+EWFQEFKEFEE FCPVTGQKLVSKAFNSN ALKS
Sbjct: 241 CPLTKNIMEDPVTLETGVSYERQAIVEWFQEFKEFEETFCPVTGQKLVSKAFNSNGALKS 300

Query: 301 TIDKWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLL 360
           TIDKWNERNE  TIEVTRD+LSLASSDEMVLE IKDLSSIS     N+EQI +FDML LL
Sbjct: 301 TIDKWNERNETPTIEVTRDSLSLASSDEMVLETIKDLSSIS-----NLEQILDFDMLQLL 360

Query: 361 VNFLEYRDRDVRYAVLQLLHQMVEVNEDNKVQIHSSCLIIVHNSLLSVMS 409
           V+FLEYRDRDVRYAVLQLLHQMVEVNEDNKVQIHSSCLII+H SLL  +S
Sbjct: 361 VDFLEYRDRDVRYAVLQLLHQMVEVNEDNKVQIHSSCLIIIHKSLLCHVS 405

BLAST of PI0009169 vs. ExPASy TrEMBL
Match: A0A6J1HVV2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467344 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.9e-149
Identity = 285/390 (73.08%), Postives = 332/390 (85.13%), Query Frame = 0

Query: 2   LRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDPI 61
           +  M T P MS AE+ILASIS+I+DS  CT EEHG FIEIGSYFYR ++A+ ELQAIDPI
Sbjct: 13  MATMKTNPNMSMAELILASISKILDSTTCT-EEHGHFIEIGSYFYRASMAIIELQAIDPI 72

Query: 62  RFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFT 121
           RFDEILQSLNKSINLAK LL+KFLT IQ  S+S+P+SII+PL+E+IK+MGECLNK+AT++
Sbjct: 73  RFDEILQSLNKSINLAKDLLEKFLTGIQQASDSEPISIIDPLKELIKQMGECLNKVATYS 132

Query: 122 FEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPID 181
           FE+QNYVKVAILSLSDEM+NI++KI  AQA  N QE++ S    S++ PEVIE DLYPID
Sbjct: 133 FEDQNYVKVAILSLSDEMQNISSKIVTAQAVINEQEMRAS---SSDEQPEVIENDLYPID 192

Query: 182 MDWDTTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPL 241
           MDWDT NTQSPV SELS+AV          KYRNVT+   K PS  +Y EPLFETFICPL
Sbjct: 193 MDWDTNNTQSPVESELSEAV----------KYRNVTAVSTKRPSTANYIEPLFETFICPL 252

Query: 242 TKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTID 301
           TK+IM DPV+LETGV+YER+AI++WF+EF+E EE+FCPVTGQKLVSKAFNSNRALKSTID
Sbjct: 253 TKDIMDDPVSLETGVTYERKAIVQWFEEFEESEEVFCPVTGQKLVSKAFNSNRALKSTID 312

Query: 302 KWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNF 361
           KW ERNE ATI+V R ALSLASSD+M+LEAIKDLSSISKGKQFNIE+IF+FDMLPLLV  
Sbjct: 313 KWKERNEIATIKVARAALSLASSDKMMLEAIKDLSSISKGKQFNIERIFDFDMLPLLVEC 372

Query: 362 LEYRDRDVRYAVLQLLHQMVEVNEDNKVQI 392
           LEYR++DVRYAVL+LLHQM E+NEDNK  I
Sbjct: 373 LEYRNKDVRYAVLELLHQMAEINEDNKKTI 388

BLAST of PI0009169 vs. ExPASy TrEMBL
Match: A0A6J1FZG7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449298 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.6e-148
Identity = 284/387 (73.39%), Postives = 329/387 (85.01%), Query Frame = 0

Query: 2   LRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDPI 61
           +  M T P MS AE+ILASIS+IIDS ACT EEHG+FIEIGSYFYR ++A+ ELQAIDPI
Sbjct: 13  MATMKTNPNMSMAELILASISKIIDSTACT-EEHGDFIEIGSYFYRASMAIIELQAIDPI 72

Query: 62  RFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFT 121
           RFDEILQSLNKSIN AK LL+KFLT IQ  S+S+P+SII+PL+E+IK+MGECLNK+AT++
Sbjct: 73  RFDEILQSLNKSINFAKDLLEKFLTGIQQASDSEPISIIDPLKELIKQMGECLNKVATYS 132

Query: 122 FEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPID 181
           FE+QNYVK+AILSLSDEM+NI++KI  AQA  N QE++ S    SE+ PEVIE DLYPID
Sbjct: 133 FEDQNYVKMAILSLSDEMQNISSKIVTAQAIINEQEMRAS---SSEEQPEVIENDLYPID 192

Query: 182 MDWDTTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPL 241
           MDWDT NTQSPV SELS+AV          KYRNVT    K PS  +Y EPLFETFICPL
Sbjct: 193 MDWDTNNTQSPVESELSEAV----------KYRNVTEVSTKRPSTANYIEPLFETFICPL 252

Query: 242 TKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTID 301
           TK+IM DPV+LETGV+YER+AI+EWF+EF+E EE+FCPVTGQKLVSKA NSNRALKSTID
Sbjct: 253 TKDIMDDPVSLETGVTYERKAIVEWFEEFEESEEVFCPVTGQKLVSKAVNSNRALKSTID 312

Query: 302 KWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNF 361
           KW ERNE ATI+V R ALSLASSD MVLEAI+DLSSISKGKQFNIE+IF+F+MLPLLV  
Sbjct: 313 KWKERNEIATIKVARAALSLASSDAMVLEAIEDLSSISKGKQFNIERIFDFEMLPLLVEC 372

Query: 362 LEYRDRDVRYAVLQLLHQMVEVNEDNK 389
           LEYR++DVRYAVL+LLHQM E+NEDNK
Sbjct: 373 LEYRNKDVRYAVLELLHQMAEINEDNK 385

BLAST of PI0009169 vs. ExPASy TrEMBL
Match: A0A6J1D4U3 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111017601 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 2.1e-148
Identity = 281/398 (70.60%), Postives = 330/398 (82.91%), Query Frame = 0

Query: 8   KPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDPIRFDEIL 67
           KPIM+ AE ILASISEII S  C+EEEH +FIEIGSYFYR +LA+ ELQAIDPI  DEI 
Sbjct: 3   KPIMTLAEPILASISEIIGSIGCSEEEHEKFIEIGSYFYRASLAIMELQAIDPINLDEIF 62

Query: 68  QSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFTFEEQNY 127
           +SL  SIN+AK L++KFLT IQ  S+  P+SII  LEEVIK+MGECL+K + +T+E+QNY
Sbjct: 63  RSLXNSINIAKDLVEKFLTGIQLTSDPKPISIIGLLEEVIKQMGECLSKTSIYTYEDQNY 122

Query: 128 VKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPIDMDWDTT 187
           VK+A+ SLSDEM+N++TK+ QAQA TN +EIQTSLEEQSEK PEVIEKDLYP+DMDWDT 
Sbjct: 123 VKMAVQSLSDEMQNMSTKVFQAQAITNEEEIQTSLEEQSEKEPEVIEKDLYPVDMDWDTK 182

Query: 188 NTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPLTKNIMK 247
           +T  P+ASE S+AV   K  RSQMKYRNVT  + KLPSM HY EPLFETF CPLTK+IM 
Sbjct: 183 DTHFPIASESSEAVTNTK-RRSQMKYRNVTESLTKLPSMAHYIEPLFETFFCPLTKDIMD 242

Query: 248 DPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTIDKWNERN 307
           DPV+LETG +YERQAI++WF+E++E EEIFCPVTGQKLVSKAFNSNRALKSTIDKW ERN
Sbjct: 243 DPVSLETGATYERQAIVDWFEEYEESEEIFCPVTGQKLVSKAFNSNRALKSTIDKWKERN 302

Query: 308 EAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNFLEYRDR 367
           E A I+V R ALSLASSDEMVLEAI+DL SI KGKQ N+E+IFNFDMLPLLV FLEY+DR
Sbjct: 303 EIARIKVARAALSLASSDEMVLEAIEDLLSICKGKQCNVERIFNFDMLPLLVKFLEYKDR 362

Query: 368 DVRYAVLQLLHQMVEVNEDNKVQIHSSCLIIVHNSLLS 406
           DVRY VL+LL QM E++ED K  I++   ++    LLS
Sbjct: 363 DVRYGVLELLQQMAEIDEDKKKMIYNQLDMLRMIKLLS 399

BLAST of PI0009169 vs. ExPASy TrEMBL
Match: A0A6P5SMU7 (RING-type E3 ubiquitin transferase OS=Prunus avium OX=42229 GN=LOC110757690 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 4.3e-85
Identity = 189/401 (47.13%), Postives = 262/401 (65.34%), Query Frame = 0

Query: 7   TKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAID--PIRFD 66
           T P+ S A+ ILASISEI +     + EH  FIEI  Y YR ++A+ EL+  D  P    
Sbjct: 7   TSPVESLADSILASISEITELVVSIKVEHESFIEIACYLYRASMAIMELKMTDTCPENAM 66

Query: 67  EILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFTFEE 126
           EILQS++KS+NLAK L+++    IQP S  +   II  LEEVIK +GECL+ I   TF +
Sbjct: 67  EILQSISKSVNLAKDLVERCQIGIQPFSYPELRIIIAQLEEVIKHIGECLSLIPPSTFGD 126

Query: 127 QNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPIDMDW 186
           Q Y +VA+  LS EM+N + +   ++AN    ++ +  E+  EK     E DLYPID + 
Sbjct: 127 QQYAEVAVRPLSKEMQNAHFEAQASEANEQDTKMLSLEEQPKEKQTPKEETDLYPIDFEV 186

Query: 187 DTTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPLTKN 246
            T N Q  +   L     + KG     K+ +++  +   P +  Y EPL+ETF CPLTK 
Sbjct: 187 STENPQ--LIEILKSTSWVSKG-----KHGSMSGSLTTFPQVGEYMEPLYETFFCPLTKK 246

Query: 247 IMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTIDKWN 306
           IM DPVT+ +GV+YER+AI+EWF++FK+ EEIFCP+TGQKL+SK+FN+N ALKST+++W 
Sbjct: 247 IMDDPVTIRSGVTYERKAIVEWFEKFKDSEEIFCPITGQKLMSKSFNANIALKSTLEEWK 306

Query: 307 ERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNFLEY 366
           ERN+AA I+V R ALSLASS+ MVLEA+KD+ SI +   ++  Q+ +  +LPLLV  LEY
Sbjct: 307 ERNQAARIKVARAALSLASSENMVLEAVKDVQSICQRNPYSKVQVRSVGILPLLVQCLEY 366

Query: 367 RDRDVRYAVLQLLHQMVEVNEDNKVQIHSSCLIIVHNSLLS 406
           +D+DVR AVL+LL Q+VE + D+K  I  +  I     +LS
Sbjct: 367 KDKDVRCAVLELLRQLVEDDNDSKEMIAQTTNIATIIKMLS 400

BLAST of PI0009169 vs. NCBI nr
Match: XP_011655725.1 (putative U-box domain-containing protein 42 isoform X2 [Cucumis sativus] >KAE8648777.1 hypothetical protein Csa_007958 [Cucumis sativus])

HSP 1 Score: 654.4 bits (1687), Expect = 6.5e-184
Identity = 347/393 (88.30%), Postives = 365/393 (92.88%), Query Frame = 0

Query: 1   MLRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDP 60
           MLRAMTTKP+MSSAEV+LASISEIIDS ACTEEEHGEFIEIGSYFYR ALAV ELQA+DP
Sbjct: 1   MLRAMTTKPMMSSAEVVLASISEIIDSTACTEEEHGEFIEIGSYFYRAALAVEELQALDP 60

Query: 61  IRFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATF 120
            +FDEILQSLNKSINLAKYLL+KFLTSIQ VSNSDP+SIINPLEEVIK+MGECLNKIAT 
Sbjct: 61  TKFDEILQSLNKSINLAKYLLEKFLTSIQLVSNSDPISIINPLEEVIKQMGECLNKIATA 120

Query: 121 TFEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPI 180
           TF+EQNYVKVAILSLSDEMKNI+ KIDQAQA  NMQEIQTSLEEQSEKVPEVIEKDLYPI
Sbjct: 121 TFKEQNYVKVAILSLSDEMKNISIKIDQAQAIMNMQEIQTSLEEQSEKVPEVIEKDLYPI 180

Query: 181 DMDWD--TTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFI 240
           DMDWD  TTNTQSPVA ELS+AV IRKGGRSQMKYRN TSDVEKLPS THYT+PLFETFI
Sbjct: 181 DMDWDTSTTNTQSPVALELSNAVIIRKGGRSQMKYRNATSDVEKLPSKTHYTKPLFETFI 240

Query: 241 CPLTKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKS 300
           CPLTKNIM+DPVTLETGVSYERQAI+EWFQEFKEFEE FCPVTGQKLVSKAFNSN ALKS
Sbjct: 241 CPLTKNIMEDPVTLETGVSYERQAIVEWFQEFKEFEETFCPVTGQKLVSKAFNSNGALKS 300

Query: 301 TIDKWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLL 360
           TIDKWNERNE  TIEVTRD+LSLASSDEMVLE IKDLSSIS     N+EQI +FDML LL
Sbjct: 301 TIDKWNERNETPTIEVTRDSLSLASSDEMVLETIKDLSSIS-----NLEQILDFDMLQLL 360

Query: 361 VNFLEYRDRDVRYAVLQLLHQMVEVNEDNKVQI 392
           V+FLEYRDRDVRYAVLQLLHQMVEVNEDNK+ I
Sbjct: 361 VDFLEYRDRDVRYAVLQLLHQMVEVNEDNKIMI 388

BLAST of PI0009169 vs. NCBI nr
Match: XP_004135423.2 (putative U-box domain-containing protein 42 isoform X1 [Cucumis sativus] >XP_031741017.1 putative U-box domain-containing protein 42 isoform X1 [Cucumis sativus])

HSP 1 Score: 654.4 bits (1687), Expect = 6.5e-184
Identity = 347/393 (88.30%), Postives = 365/393 (92.88%), Query Frame = 0

Query: 1   MLRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDP 60
           MLRAMTTKP+MSSAEV+LASISEIIDS ACTEEEHGEFIEIGSYFYR ALAV ELQA+DP
Sbjct: 1   MLRAMTTKPMMSSAEVVLASISEIIDSTACTEEEHGEFIEIGSYFYRAALAVEELQALDP 60

Query: 61  IRFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATF 120
            +FDEILQSLNKSINLAKYLL+KFLTSIQ VSNSDP+SIINPLEEVIK+MGECLNKIAT 
Sbjct: 61  TKFDEILQSLNKSINLAKYLLEKFLTSIQLVSNSDPISIINPLEEVIKQMGECLNKIATA 120

Query: 121 TFEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPI 180
           TF+EQNYVKVAILSLSDEMKNI+ KIDQAQA  NMQEIQTSLEEQSEKVPEVIEKDLYPI
Sbjct: 121 TFKEQNYVKVAILSLSDEMKNISIKIDQAQAIMNMQEIQTSLEEQSEKVPEVIEKDLYPI 180

Query: 181 DMDWD--TTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFI 240
           DMDWD  TTNTQSPVA ELS+AV IRKGGRSQMKYRN TSDVEKLPS THYT+PLFETFI
Sbjct: 181 DMDWDTSTTNTQSPVALELSNAVIIRKGGRSQMKYRNATSDVEKLPSKTHYTKPLFETFI 240

Query: 241 CPLTKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKS 300
           CPLTKNIM+DPVTLETGVSYERQAI+EWFQEFKEFEE FCPVTGQKLVSKAFNSN ALKS
Sbjct: 241 CPLTKNIMEDPVTLETGVSYERQAIVEWFQEFKEFEETFCPVTGQKLVSKAFNSNGALKS 300

Query: 301 TIDKWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLL 360
           TIDKWNERNE  TIEVTRD+LSLASSDEMVLE IKDLSSIS     N+EQI +FDML LL
Sbjct: 301 TIDKWNERNETPTIEVTRDSLSLASSDEMVLETIKDLSSIS-----NLEQILDFDMLQLL 360

Query: 361 VNFLEYRDRDVRYAVLQLLHQMVEVNEDNKVQI 392
           V+FLEYRDRDVRYAVLQLLHQMVEVNEDNK+ I
Sbjct: 361 VDFLEYRDRDVRYAVLQLLHQMVEVNEDNKIMI 388

BLAST of PI0009169 vs. NCBI nr
Match: XP_038892389.1 (putative U-box domain-containing protein 42 [Benincasa hispida])

HSP 1 Score: 605.1 bits (1559), Expect = 4.5e-169
Identity = 320/391 (81.84%), Postives = 346/391 (88.49%), Query Frame = 0

Query: 1   MLRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDP 60
           M RAMTTKP +S AE+IL SISEIIDS ACTEEEHG+ IEIGSYFYR ALAV ELQAIDP
Sbjct: 1   MSRAMTTKPKISLAELILTSISEIIDSKACTEEEHGKLIEIGSYFYRAALAVAELQAIDP 60

Query: 61  IRFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATF 120
           I+FDEILQSLNKSIN AK L++KF   IQPVS+SDP+SIINPLEEVIK+MGECLNKIA  
Sbjct: 61  IKFDEILQSLNKSINHAKELVEKFQNGIQPVSDSDPISIINPLEEVIKQMGECLNKIAIA 120

Query: 121 TFEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPI 180
           TFEEQ+YVK+AILSLSDEMKNI+TKI QAQA  N QEIQTSLEEQSEK PEVIE+DLYPI
Sbjct: 121 TFEEQSYVKMAILSLSDEMKNISTKIVQAQAIMNKQEIQTSLEEQSEKEPEVIERDLYPI 180

Query: 181 DMDWDTTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICP 240
           DMDWDT NTQS V SE +       G RSQMKYRNVTS +EKLP M HY EPLFETFICP
Sbjct: 181 DMDWDTNNTQSSVVSESN-----TNGRRSQMKYRNVTSSMEKLPLMNHYIEPLFETFICP 240

Query: 241 LTKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTI 300
           LTKNIM DPV+LETGVSYERQAI+EW +EFKE EEIFCPVTGQKLVSKAFNSNRALKSTI
Sbjct: 241 LTKNIMDDPVSLETGVSYERQAIVEWLEEFKESEEIFCPVTGQKLVSKAFNSNRALKSTI 300

Query: 301 DKWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVN 360
           +KW ERNE ATI+VTR ALSLASSD+MVLEAIKDLSSISKGKQFNIE+IFNF MLPLL+N
Sbjct: 301 EKWKERNEIATIKVTRAALSLASSDDMVLEAIKDLSSISKGKQFNIERIFNFGMLPLLIN 360

Query: 361 FLEYRDRDVRYAVLQLLHQMVEVNEDNKVQI 392
           FLEYRDRDVRYAVL+LLHQM E+NE+NKV I
Sbjct: 361 FLEYRDRDVRYAVLELLHQMAEINEENKVTI 386

BLAST of PI0009169 vs. NCBI nr
Match: XP_023542844.1 (putative U-box domain-containing protein 42 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.0 bits (1390), Expect = 1.8e-149
Identity = 286/387 (73.90%), Postives = 329/387 (85.01%), Query Frame = 0

Query: 2   LRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDPI 61
           +  M T P MS AE+ILASIS+IIDS  CT EEHG FIEIGSYFYR ++A+ ELQAIDPI
Sbjct: 13  MATMKTNPNMSMAELILASISKIIDSTTCT-EEHGHFIEIGSYFYRASMAIIELQAIDPI 72

Query: 62  RFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFT 121
           RFDEILQSLNKSINLAK LL+KFLT IQ  S+S+P+SII+PL+E+IK+MGECLNK+AT++
Sbjct: 73  RFDEILQSLNKSINLAKDLLEKFLTGIQQASDSEPISIIDPLKELIKQMGECLNKVATYS 132

Query: 122 FEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPID 181
           FE+QNYVK+AILSLSDEM+NI++KI  AQA  N QE++ S    S++ PEVIE DLYPID
Sbjct: 133 FEDQNYVKMAILSLSDEMQNISSKIVTAQAVINEQEMRAS---SSDEQPEVIENDLYPID 192

Query: 182 MDWDTTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPL 241
           MDWDT NTQSPV SELS+AV          KYRNVT    K PS  +Y EPLFETFICPL
Sbjct: 193 MDWDTNNTQSPVESELSEAV----------KYRNVTEVSTKRPSTANYIEPLFETFICPL 252

Query: 242 TKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTID 301
           TK+IM DPV+LETGV+YER+AI+EWF+EF+E EE+FCPVTGQKLVSKA NSNRALKSTID
Sbjct: 253 TKDIMDDPVSLETGVTYERKAIVEWFEEFEESEEVFCPVTGQKLVSKAVNSNRALKSTID 312

Query: 302 KWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNF 361
           KW ERNE ATI+V R ALSLASSDEMVLEAIKDLSSISKGKQFNIE+IFNFDMLPLLV  
Sbjct: 313 KWKERNEIATIKVARAALSLASSDEMVLEAIKDLSSISKGKQFNIERIFNFDMLPLLVKC 372

Query: 362 LEYRDRDVRYAVLQLLHQMVEVNEDNK 389
           LEYR++DVRYA+L+LLHQM E+NEDNK
Sbjct: 373 LEYRNKDVRYALLELLHQMAEINEDNK 385

BLAST of PI0009169 vs. NCBI nr
Match: XP_022967965.1 (putative U-box domain-containing protein 42 [Cucurbita maxima] >XP_022967966.1 putative U-box domain-containing protein 42 [Cucurbita maxima])

HSP 1 Score: 538.9 bits (1387), Expect = 4.0e-149
Identity = 285/390 (73.08%), Postives = 332/390 (85.13%), Query Frame = 0

Query: 2   LRAMTTKPIMSSAEVILASISEIIDSAACTEEEHGEFIEIGSYFYRVALAVGELQAIDPI 61
           +  M T P MS AE+ILASIS+I+DS  CT EEHG FIEIGSYFYR ++A+ ELQAIDPI
Sbjct: 13  MATMKTNPNMSMAELILASISKILDSTTCT-EEHGHFIEIGSYFYRASMAIIELQAIDPI 72

Query: 62  RFDEILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFT 121
           RFDEILQSLNKSINLAK LL+KFLT IQ  S+S+P+SII+PL+E+IK+MGECLNK+AT++
Sbjct: 73  RFDEILQSLNKSINLAKDLLEKFLTGIQQASDSEPISIIDPLKELIKQMGECLNKVATYS 132

Query: 122 FEEQNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPID 181
           FE+QNYVKVAILSLSDEM+NI++KI  AQA  N QE++ S    S++ PEVIE DLYPID
Sbjct: 133 FEDQNYVKVAILSLSDEMQNISSKIVTAQAVINEQEMRAS---SSDEQPEVIENDLYPID 192

Query: 182 MDWDTTNTQSPVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPL 241
           MDWDT NTQSPV SELS+AV          KYRNVT+   K PS  +Y EPLFETFICPL
Sbjct: 193 MDWDTNNTQSPVESELSEAV----------KYRNVTAVSTKRPSTANYIEPLFETFICPL 252

Query: 242 TKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTID 301
           TK+IM DPV+LETGV+YER+AI++WF+EF+E EE+FCPVTGQKLVSKAFNSNRALKSTID
Sbjct: 253 TKDIMDDPVSLETGVTYERKAIVQWFEEFEESEEVFCPVTGQKLVSKAFNSNRALKSTID 312

Query: 302 KWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNF 361
           KW ERNE ATI+V R ALSLASSD+M+LEAIKDLSSISKGKQFNIE+IF+FDMLPLLV  
Sbjct: 313 KWKERNEIATIKVARAALSLASSDKMMLEAIKDLSSISKGKQFNIERIFDFDMLPLLVEC 372

Query: 362 LEYRDRDVRYAVLQLLHQMVEVNEDNKVQI 392
           LEYR++DVRYAVL+LLHQM E+NEDNK  I
Sbjct: 373 LEYRNKDVRYAVLELLHQMAEINEDNKKTI 388

BLAST of PI0009169 vs. TAIR 10
Match: AT1G68940.1 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 202.2 bits (513), Expect = 8.2e-52
Identity = 134/401 (33.42%), Postives = 204/401 (50.87%), Query Frame = 0

Query: 65  EILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFTFEE 124
           +I +SL+ S+++AK L++K   S +  S +D  SI    E V+K+MGE L  I   TF+E
Sbjct: 15  DIFESLSDSVDVAKKLVEKSQESNEAESTTDLRSIEAGFEGVVKQMGETLQSIPESTFDE 74

Query: 125 QNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPIDMDW 184
           + Y+ V I SLS+EM+N            N  + + S +   + V E +E+DLYP D ++
Sbjct: 75  EEYIGVVIQSLSNEMQNATIGDGSKSEMINNGQQKISAKHTPDIVSEQMEEDLYPTDPEF 134

Query: 185 ----------------------------------------------------DTTNTQS- 244
                                                                +TN  S 
Sbjct: 135 SYESYMMYSESQSQMTDIPDIPSKSTDVSRQRKHGNHSESQSLVTEIPDIPSQSTNVSSQ 194

Query: 245 ----------PVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPL 304
                       ++E+ D  +      SQ KY N++  +  LP +T + EP ++ FICPL
Sbjct: 195 RKHGNLSKSQSQSTEIPDIPSQSSNASSQRKYGNLSESLSMLPQVTQFMEPPYQAFICPL 254

Query: 305 TKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTID 364
           TK IM+DPVT ETGV+ ERQA++EWF  F   +EI CPVTGQKL ++  ++N  LK+ I 
Sbjct: 255 TKEIMEDPVTTETGVTCERQAVIEWFDSFGNSDEINCPVTGQKLTTE-LSANVVLKTIIQ 314

Query: 365 KWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNF 398
           +W  RNEAA I+V   ALSL  S+ MV++A++DL    +GK++N  Q+    ++ LL  +
Sbjct: 315 EWKVRNEAARIKVAHAALSLGGSESMVIDALRDLQMTCEGKEYNKVQVREAGIIQLLDRY 374

BLAST of PI0009169 vs. TAIR 10
Match: AT1G68940.2 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 202.2 bits (513), Expect = 8.2e-52
Identity = 134/401 (33.42%), Postives = 204/401 (50.87%), Query Frame = 0

Query: 65  EILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFTFEE 124
           +I +SL+ S+++AK L++K   S +  S +D  SI    E V+K+MGE L  I   TF+E
Sbjct: 15  DIFESLSDSVDVAKKLVEKSQESNEAESTTDLRSIEAGFEGVVKQMGETLQSIPESTFDE 74

Query: 125 QNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPIDMDW 184
           + Y+ V I SLS+EM+N            N  + + S +   + V E +E+DLYP D ++
Sbjct: 75  EEYIGVVIQSLSNEMQNATIGDGSKSEMINNGQQKISAKHTPDIVSEQMEEDLYPTDPEF 134

Query: 185 ----------------------------------------------------DTTNTQS- 244
                                                                +TN  S 
Sbjct: 135 SYESYMMYSESQSQMTDIPDIPSKSTDVSRQRKHGNHSESQSLVTEIPDIPSQSTNVSSQ 194

Query: 245 ----------PVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPL 304
                       ++E+ D  +      SQ KY N++  +  LP +T + EP ++ FICPL
Sbjct: 195 RKHGNLSKSQSQSTEIPDIPSQSSNASSQRKYGNLSESLSMLPQVTQFMEPPYQAFICPL 254

Query: 305 TKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTID 364
           TK IM+DPVT ETGV+ ERQA++EWF  F   +EI CPVTGQKL ++  ++N  LK+ I 
Sbjct: 255 TKEIMEDPVTTETGVTCERQAVIEWFDSFGNSDEINCPVTGQKLTTE-LSANVVLKTIIQ 314

Query: 365 KWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNF 398
           +W  RNEAA I+V   ALSL  S+ MV++A++DL    +GK++N  Q+    ++ LL  +
Sbjct: 315 EWKVRNEAARIKVAHAALSLGGSESMVIDALRDLQMTCEGKEYNKVQVREAGIIQLLDRY 374

BLAST of PI0009169 vs. TAIR 10
Match: AT1G68940.3 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 202.2 bits (513), Expect = 8.2e-52
Identity = 134/401 (33.42%), Postives = 204/401 (50.87%), Query Frame = 0

Query: 65  EILQSLNKSINLAKYLLDKFLTSIQPVSNSDPVSIINPLEEVIKEMGECLNKIATFTFEE 124
           +I +SL+ S+++AK L++K   S +  S +D  SI    E V+K+MGE L  I   TF+E
Sbjct: 15  DIFESLSDSVDVAKKLVEKSQESNEAESTTDLRSIEAGFEGVVKQMGETLQSIPESTFDE 74

Query: 125 QNYVKVAILSLSDEMKNINTKIDQAQANTNMQEIQTSLEEQSEKVPEVIEKDLYPIDMDW 184
           + Y+ V I SLS+EM+N            N  + + S +   + V E +E+DLYP D ++
Sbjct: 75  EEYIGVVIQSLSNEMQNATIGDGSKSEMINNGQQKISAKHTPDIVSEQMEEDLYPTDPEF 134

Query: 185 ----------------------------------------------------DTTNTQS- 244
                                                                +TN  S 
Sbjct: 135 SYESYMMYSESQSQMTDIPDIPSKSTDVSRQRKHGNHSESQSLVTEIPDIPSQSTNVSSQ 194

Query: 245 ----------PVASELSDAVNIRKGGRSQMKYRNVTSDVEKLPSMTHYTEPLFETFICPL 304
                       ++E+ D  +      SQ KY N++  +  LP +T + EP ++ FICPL
Sbjct: 195 RKHGNLSKSQSQSTEIPDIPSQSSNASSQRKYGNLSESLSMLPQVTQFMEPPYQAFICPL 254

Query: 305 TKNIMKDPVTLETGVSYERQAILEWFQEFKEFEEIFCPVTGQKLVSKAFNSNRALKSTID 364
           TK IM+DPVT ETGV+ ERQA++EWF  F   +EI CPVTGQKL ++  ++N  LK+ I 
Sbjct: 255 TKEIMEDPVTTETGVTCERQAVIEWFDSFGNSDEINCPVTGQKLTTE-LSANVVLKTIIQ 314

Query: 365 KWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFNFDMLPLLVNF 398
           +W  RNEAA I+V   ALSL  S+ MV++A++DL    +GK++N  Q+    ++ LL  +
Sbjct: 315 EWKVRNEAARIKVAHAALSLGGSESMVIDALRDLQMTCEGKEYNKVQVREAGIIQLLDRY 374

BLAST of PI0009169 vs. TAIR 10
Match: AT1G20780.1 (senescence-associated E3 ubiquitin ligase 1 )

HSP 1 Score: 116.3 bits (290), Expect = 5.9e-26
Identity = 59/176 (33.52%), Postives = 111/176 (63.07%), Query Frame = 0

Query: 217 TSDVEKLPSMTHY---TEPLFETFICPLTKNIMKDPVTLETGVSYERQAILEWFQEFKEF 276
           +SD ++    +H+    + ++E FICPLTK +M DPVTLE G ++ER+AI +WF+E ++ 
Sbjct: 4   SSDGDQSDDSSHFERGVDHIYEAFICPLTKEVMHDPVTLENGRTFEREAIEKWFKECRDS 63

Query: 277 -EEIFCPVTGQKLVSKAFNSNRALKSTIDKWNERNEAATIEVTRDALSLASSDEMVLEAI 336
                CP+T Q+L S   +++ AL++TI++W  RN+AA +++ R +L L +++  +L+A+
Sbjct: 64  GRPPSCPLTSQELTSTDVSASIALRNTIEEWRSRNDAAKLDIARQSLFLGNAETDILQAL 123

Query: 337 KDLSSISKGKQFNIEQIFNFDMLPLLVNFLEYRDRDVRYAVLQLLHQMVEVNEDNK 389
             +  I +  + N   + N  ++ ++++ L+     VRY  LQ L  +VE ++++K
Sbjct: 124 MHVRQICRTIRSNRHGVRNSQLIHMIIDMLKSTSHRVRYKALQTLQVVVEGDDESK 179

BLAST of PI0009169 vs. TAIR 10
Match: AT1G76390.1 (ARM repeat superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 5.5e-24
Identity = 55/157 (35.03%), Postives = 102/157 (64.97%), Query Frame = 0

Query: 233 LFETFICPLTKNIMKDPVTLETGVSYERQAILEWFQEFKE-FEEIFCPVTGQKLVSKAFN 292
           ++E FICPLTK +M +PVTLE G ++ER+AI +WFQE +E  + + CP+T ++L     +
Sbjct: 25  IYEAFICPLTKQVMHNPVTLENGQTFEREAIEKWFQECRENGQPLSCPITSKELSITDLS 84

Query: 293 SNRALKSTIDKWNERNEAATIEVTRDALSLASSDEMVLEAIKDLSSISKGKQFNIEQIFN 352
            + AL++TI++W  RN+A  +++ R +L L +++  +L A+K++  I +  +   +++ N
Sbjct: 85  PSIALRNTIEEWRARNDALKLDIARQSLYLGNAETNILLALKNVREICRNIRKIRQRVCN 144

Query: 353 FDMLPLLVNFLEYRDRDVRYAVLQLLHQMVEVNEDNK 389
             ++ L+ + L+    +VR   LQ L  +VE +E++K
Sbjct: 145 PQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDEESK 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9CAA71.1e-5033.42Putative U-box domain-containing protein 42 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9LM768.3e-2533.52U-box domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN=PUB44 PE=1... [more]
Q9SFX27.8e-2335.03U-box domain-containing protein 43 OS=Arabidopsis thaliana OX=3702 GN=PUB43 PE=2... [more]
Q10FT07.5e-1833.72U-box domain-containing protein 24 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q681N21.3e-0930.18U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0KT189.1e-19288.05RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G607430 PE... [more]
A0A6J1HVV21.9e-14973.08RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467344 P... [more]
A0A6J1FZG71.6e-14873.39RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449298... [more]
A0A6J1D4U32.1e-14870.60RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101760... [more]
A0A6P5SMU74.3e-8547.13RING-type E3 ubiquitin transferase OS=Prunus avium OX=42229 GN=LOC110757690 PE=4... [more]
Match NameE-valueIdentityDescription
XP_011655725.16.5e-18488.30putative U-box domain-containing protein 42 isoform X2 [Cucumis sativus] >KAE864... [more]
XP_004135423.26.5e-18488.30putative U-box domain-containing protein 42 isoform X1 [Cucumis sativus] >XP_031... [more]
XP_038892389.14.5e-16981.84putative U-box domain-containing protein 42 [Benincasa hispida][more]
XP_023542844.11.8e-14973.90putative U-box domain-containing protein 42 [Cucurbita pepo subsp. pepo][more]
XP_022967965.14.0e-14973.08putative U-box domain-containing protein 42 [Cucurbita maxima] >XP_022967966.1 p... [more]
Match NameE-valueIdentityDescription
AT1G68940.18.2e-5233.42Armadillo/beta-catenin-like repeat family protein [more]
AT1G68940.28.2e-5233.42Armadillo/beta-catenin-like repeat family protein [more]
AT1G68940.38.2e-5233.42Armadillo/beta-catenin-like repeat family protein [more]
AT1G20780.15.9e-2633.52senescence-associated E3 ubiquitin ligase 1 [more]
AT1G76390.15.5e-2435.03ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 293..313
NoneNo IPR availableCOILSCoilCoilcoord: 132..168
NoneNo IPR availablePANTHERPTHR45958:SF4U-BOX DOMAIN-CONTAINING PROTEIN 42-RELATEDcoord: 9..405
NoneNo IPR availablePANTHERPTHR45958RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 9..405
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 237..283
e-value: 1.60179E-17
score: 73.8071
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 224..312
IPR003613U box domainSMARTSM00504Ubox_2coord: 236..303
e-value: 6.5E-24
score: 95.5
IPR003613U box domainPFAMPF04564U-boxcoord: 235..308
e-value: 5.5E-15
score: 55.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 232..310
score: 20.293802
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 318..405
e-value: 3.6E-7
score: 31.7
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 215..317
e-value: 8.9E-23
score: 82.1
IPR021133HEAT, type 2PROSITEPS50077HEAT_REPEATcoord: 354..392
score: 9.084101
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 322..394

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009169.1PI0009169.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity