PI0009100 (gene) Melon (PI 482460) v1

Overview


Name	PI0009100
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	MYB transcription factor
Location	chr12: 197783 .. 198810 (-)
RNA-Seq Expression	PI0009100
Synteny	PI0009100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAAAATAAGATCTGAATTTAGTTTAGGATAATGTGCAGCAGAGGGCACTGGCGGCCTGCAGAAGACGAGAAGCTTCGAGAGCTGGTGGAGCGTTACGGACCTCATAACTGGAACGCCATAGCTCAAAAGCTTCAAGGAAGATCTGGGAAGAGCTGCCGCTTGAGATGGTTCAATCAGTTGGATCCTCGAATCAACAGAACCCCTTTCACCGAGGAGGAAGAAGAGAGGCTGATGGCGTCCCACAGGGTCCATGGCAACAGATGGGCCATCATCGCAAGGCTTTTCCCCGGCCGCACCGATAACGCAGTCAAGAACCACTGGCACGTCATCATGGCTCGTAGGTCTAGAAATAAATCCAAAACCCAAACCCTAACACAAACCCTAACCCAAACACACAATAATAATAATAATAATAATCGTCTTCTTCTTTCTTCCTTTCTCCAACTCCATGCATATCCCACTTCTAATATTTCATTCATCCCCAAACCACCATCTGGTAATTAATTATATACATTATTCTTTCTTTTTTTTTTCTTACATATGTGTGTGTGTGTGCGTTTTCATTATTATAATTTATATATTAATTATATGTATCTATGGCAGATGATGCAGCAGCAGCAGCAGCGCATAGTAGTTGTTCCTTTATTCATTATTATGACTTTCTCCAAGTAAATAGTACGGATTCTATGAATGGAAGTGAAGTGATAGATAACTCATCAAGAAAAGAGGATTACGAAGAGGTGAATAATAATAATAATGGAGAACAACAGGCGGTACTACTTCCCTTCATTGATTTTTTTTCTGCCTCCAATGACGACACATCCATCTCATGATCCTCCTAAACATGTATATATATATATATATATATATCCAACCCAAAAAATTAAACCTCCTTTATATATATATCTCACATTTCCTCTAACTCACTTGGGGCATCATGAAAATCACAATACCCCGCCCCCATATATACATACATACCATATATATTGTATCCATTATAATGCTGCTAATTAATTTGTACACAAT

mRNA sequence

ATCAAAATAAGATCTGAATTTAGTTTAGGATAATGTGCAGCAGAGGGCACTGGCGGCCTGCAGAAGACGAGAAGCTTCGAGAGCTGGTGGAGCGTTACGGACCTCATAACTGGAACGCCATAGCTCAAAAGCTTCAAGGAAGATCTGGGAAGAGCTGCCGCTTGAGATGGTTCAATCAGTTGGATCCTCGAATCAACAGAACCCCTTTCACCGAGGAGGAAGAAGAGAGGCTGATGGCGTCCCACAGGGTCCATGGCAACAGATGGGCCATCATCGCAAGGCTTTTCCCCGGCCGCACCGATAACGCAGTCAAGAACCACTGGCACGTCATCATGGCTCGTAGGTCTAGAAATAAATCCAAAACCCAAACCCTAACACAAACCCTAACCCAAACACACAATAATAATAATAATAATAATCGTCTTCTTCTTTCTTCCTTTCTCCAACTCCATGCATATCCCACTTCTAATATTTCATTCATCCCCAAACCACCATCTGATGATGCAGCAGCAGCAGCAGCGCATAGTAGTTGTTCCTTTATTCATTATTATGACTTTCTCCAAGTAAATAGTACGGATTCTATGAATGGAAGTGAAGTGATAGATAACTCATCAAGAAAAGAGGATTACGAAGAGGTGAATAATAATAATAATGGAGAACAACAGGCGGTACTACTTCCCTTCATTGATTTTTTTTCTGCCTCCAATGACGACACATCCATCTCATGATCCTCCTAAACATGTATATATATATATATATATATATCCAACCCAAAAAATTAAACCTCCTTTATATATATATCTCACATTTCCTCTAACTCACTTGGGGCATCATGAAAATCACAATACCCCGCCCCCATATATACATACATACCATATATATTGTATCCATTATAATGCTGCTAATTAATTTGTACACAAT

Coding sequence (CDS)

ATGTGCAGCAGAGGGCACTGGCGGCCTGCAGAAGACGAGAAGCTTCGAGAGCTGGTGGAGCGTTACGGACCTCATAACTGGAACGCCATAGCTCAAAAGCTTCAAGGAAGATCTGGGAAGAGCTGCCGCTTGAGATGGTTCAATCAGTTGGATCCTCGAATCAACAGAACCCCTTTCACCGAGGAGGAAGAAGAGAGGCTGATGGCGTCCCACAGGGTCCATGGCAACAGATGGGCCATCATCGCAAGGCTTTTCCCCGGCCGCACCGATAACGCAGTCAAGAACCACTGGCACGTCATCATGGCTCGTAGGTCTAGAAATAAATCCAAAACCCAAACCCTAACACAAACCCTAACCCAAACACACAATAATAATAATAATAATAATCGTCTTCTTCTTTCTTCCTTTCTCCAACTCCATGCATATCCCACTTCTAATATTTCATTCATCCCCAAACCACCATCTGATGATGCAGCAGCAGCAGCAGCGCATAGTAGTTGTTCCTTTATTCATTATTATGACTTTCTCCAAGTAAATAGTACGGATTCTATGAATGGAAGTGAAGTGATAGATAACTCATCAAGAAAAGAGGATTACGAAGAGGTGAATAATAATAATAATGGAGAACAACAGGCGGTACTACTTCCCTTCATTGATTTTTTTTCTGCCTCCAATGACGACACATCCATCTCATGA

Protein sequence

MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFTEEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQTHNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPPSDDAAAAAAHSSCSFIHYYDFLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSASNDDTSIS

Homology

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BLAST of PI0009100 vs. ExPASy Swiss-Prot
Match: Q6R0C4 (Transcription factor MYB52 OS=Arabidopsis thaliana OX=3702 GN=MYB52 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 7.4e-60
Identity = 131/247 (53.04%), Postives = 163/247 (65.99%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKLRELVE++GPHNWNAIAQKL GRSGKSCRLRWFNQLDPRINR PFT
Sbjct: 2   MCSRGHWRPAEDEKLRELVEQFGPHNWNAIAQKLSGRSGKSCRLRWFNQLDPRINRNPFT 61

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQ-------- 120
           EEEEERL+ASHR+HGNRW++IAR FPGRTDNAVKNHWHVIMARR R +SK +        
Sbjct: 62  EEEEERLLASHRIHGNRWSVIARFFPGRTDNAVKNHWHVIMARRGRERSKLRPRGLGHDG 121

Query: 121 TLTQT-LTQTHNNNNNNNRLL-------------LSSFLQLHAYPTSNISFIPKPPSDDA 180
           T+  T +   + + +   RL              ++ F  L  + T  I F  +  +   
Sbjct: 122 TVAATGMIGNYKDCDKERRLATTTAINFPYQFSHINHFQVLKEFLTGKIGF--RNSTTPI 181

Query: 181 AAAAAHSSCSFIHYYDFLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFI 226
              A   +   + +Y+FLQVN+   ++  E+IDN SRK++ E+V+ NN    +   +PF 
Sbjct: 182 QEGAIDQTKRPMEFYNFLQVNTDSKIH--ELIDN-SRKDEEEDVDQNNRIPNEN-CVPFF 241

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BLAST of PI0009100 vs. ExPASy Swiss-Prot
Match: Q9FX36 (Transcription factor MYB54 OS=Arabidopsis thaliana OX=3702 GN=MYB54 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.4e-58
Identity = 128/245 (52.24%), Postives = 159/245 (64.90%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKL++LVE+YGPHNWNAIA KL GRSGKSCRLRWFNQLDPRINR PFT
Sbjct: 3   MCSRGHWRPAEDEKLKDLVEQYGPHNWNAIALKLPGRSGKSCRLRWFNQLDPRINRNPFT 62

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERL+A+HR+HGNRW+IIARLFPGRTDNAVKNHWHVIMARR+R  SK + L  T + 
Sbjct: 63  EEEEERLLAAHRIHGNRWSIIARLFPGRTDNAVKNHWHVIMARRTRQTSKPRLLPSTTSS 122

Query: 121 T----------------HNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPPSDDAAAAA-- 180
           +                HN ++++ + +  +      Y  S+I+ +           A  
Sbjct: 123 SSLMASEQIMMSSGGYNHNYSSDDRKKIFPADFINFPYKFSHINHLHFLKEFFTGKIALN 182

Query: 181 --AHSSCSFIHYYDFLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDF 226
             A+ S   + +Y+FLQVN TDS N SE+ID  S +    + +  +        +PF DF
Sbjct: 183 HKANQSKKPMEFYNFLQVN-TDS-NKSEIIDQDSGQSKRSDSDTKHESH-----VPFFDF 240

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BLAST of PI0009100 vs. ExPASy Swiss-Prot
Match: Q5NBM8 (Transcription factor CSA OS=Oryza sativa subsp. japonica OX=39947 GN=CSA PE=2 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 5.3e-50
Identity = 87/109 (79.82%), Postives = 99/109 (90.83%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           +C+RGHWRPAED KL++LV +YGP NWN IA+KL GRSGKSCRLRWFNQLDPRINR  FT
Sbjct: 46  LCARGHWRPAEDAKLKDLVAQYGPQNWNLIAEKLDGRSGKSCRLRWFNQLDPRINRRAFT 105

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKS 110
           EEEEERLMA+HR +GN+WA+IARLFPGRTDNAVKNHWHV+MARR R +S
Sbjct: 106 EEEEERLMAAHRAYGNKWALIARLFPGRTDNAVKNHWHVLMARRHREQS 154

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BLAST of PI0009100 vs. ExPASy Swiss-Prot
Match: Q9SEZ4 (Transcription factor MYB105 OS=Arabidopsis thaliana OX=3702 GN=MYB105 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.2e-48
Identity = 88/116 (75.86%), Postives = 101/116 (87.07%), Query Frame = 0


Query: 3   SRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFTEE 62
           SRGHWRPAED KL+ELV  YGP NWN IA+KLQGRSGKSCRLRWFNQLDPRINR  FTEE
Sbjct: 106 SRGHWRPAEDTKLKELVAVYGPQNWNLIAEKLQGRSGKSCRLRWFNQLDPRINRRAFTEE 165

Query: 63  EEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTL 119
           EEERLM +HR++GN+WA+IARLFPGRTDN+VKNHWHVIMAR+ R +S +    +T+
Sbjct: 166 EEERLMQAHRLYGNKWAMIARLFPGRTDNSVKNHWHVIMARKFREQSSSYRRRKTM 221

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BLAST of PI0009100 vs. ExPASy Swiss-Prot
Match: Q9LQX5 (Transcription factor MYB117 OS=Arabidopsis thaliana OX=3702 GN=MYB117 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 7.2e-47
Identity = 95/148 (64.19%), Postives = 108/148 (72.97%), Query Frame = 0


Query: 4   RGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFTEEE 63
           RGHWRPAED KL+ELV  YGP NWN IA+KLQGRSGKSCRLRWFNQLDPRINR  FTEEE
Sbjct: 98  RGHWRPAEDVKLKELVSIYGPQNWNLIAEKLQGRSGKSCRLRWFNQLDPRINRRAFTEEE 157

Query: 64  EERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKS---------KTQTL 123
           EERLM +HR++GN+WA+IARLFPGRTDN+VKNHWHV+MAR+ R  S             L
Sbjct: 158 EERLMQAHRLYGNKWAMIARLFPGRTDNSVKNHWHVVMARKYREHSSAYRRRKLMSNNPL 217

Query: 124 TQTLTQTHNNNNNNNRLLLSSFLQLHAY 143
              LT  H+ N N N     SF+  + Y
Sbjct: 218 KPHLTNNHHPNPNPN---YHSFISTNHY 242

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BLAST of PI0009100 vs. ExPASy TrEMBL
Match: A0A0A0LPV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G029570 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 3.4e-124
Identity = 226/232 (97.41%), Postives = 229/232 (98.71%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT
Sbjct: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ
Sbjct: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120

Query: 121 THNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPPSDD-AAAAAAHSSCSFIHYYDFLQVN 180
           THNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPP DD AAAAAAHSSCS IHYYDFLQVN
Sbjct: 121 THNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPPPDDAAAAAAAHSSCSIIHYYDFLQVN 180

Query: 181 STDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSASNDDTSIS 232
           STDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSAS+++TSIS
Sbjct: 181 STDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSASSNNTSIS 232

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BLAST of PI0009100 vs. ExPASy TrEMBL
Match: A0A1S3C1Q2 (transcriptional activator Myb-like OS=Cucumis melo OX=3656 GN=LOC103495663 PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.3e-120
Identity = 224/234 (95.73%), Postives = 228/234 (97.44%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINR+PFT
Sbjct: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRSPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ
Sbjct: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120

Query: 121 THNNN-NNNNRLLLSSFLQLHAYPTSNISFIPKPPSDD-AAAAAAHSSCSFIHYYDFLQV 180
           THNNN NNN+RLLLSSFLQLHAYPTS ISF+PKPPSDD AAAAAAHSSCS IHYYDFLQV
Sbjct: 121 THNNNHNNNHRLLLSSFLQLHAYPTSTISFVPKPPSDDAAAAAAAHSSCSIIHYYDFLQV 180

Query: 181 NSTDSMNGSEVIDNSSRKEDYEEVNNNNN-GEQQAVLLPFIDFFSASNDDTSIS 232
           NSTDS NGSEVIDNSSRKEDYEEVNNNNN GEQQAVLLPFIDFFSASN+DTSIS
Sbjct: 181 NSTDSTNGSEVIDNSSRKEDYEEVNNNNNSGEQQAVLLPFIDFFSASNNDTSIS 234

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BLAST of PI0009100 vs. ExPASy TrEMBL
Match: A0A6J1KP91 (transcription factor MYB52-like OS=Cucurbita maxima OX=3661 GN=LOC111495269 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 1.0e-72
Identity = 155/246 (63.01%), Postives = 176/246 (71.54%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAED+KLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINR+PFT
Sbjct: 1   MCSRGHWRPAEDDKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRSPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRN----KSKTQTLTQ 120
           EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMAR+SR+    K+K +   +
Sbjct: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARKSRDSSKAKAKAKAKAK 120

Query: 121 TLTQTHN-NNNNNNRLLLSSFLQLHA-------------YPTSNISFIPKPPSDDAAAAA 180
            +T+T   + ++ + LLLSSFLQ                YP    SFIPKP         
Sbjct: 121 AITKTQTLSPSSQSHLLLSSFLQTSCPPNNASLFLNHSLYPNPISSFIPKPTDRS----- 180

Query: 181 AHSSCSFIHYYDFLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFS 229
                  + +YDFL +N+ +S+N SEVIDN SRKEDYEEVN          L PFIDFFS
Sbjct: 181 -------VEFYDFLHLNA-ESINASEVIDN-SRKEDYEEVNQEATATAARPLFPFIDFFS 232

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BLAST of PI0009100 vs. ExPASy TrEMBL
Match: A0A6J1EDT2 (transcription factor MYB52-like OS=Cucurbita moschata OX=3662 GN=LOC111431625 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 3.0e-72
Identity = 152/235 (64.68%), Postives = 170/235 (72.34%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAED+KLRELVERYGPHNWNAIAQKL GRSGKSCRLRWFNQLDPRINR+PFT
Sbjct: 1   MCSRGHWRPAEDDKLRELVERYGPHNWNAIAQKLLGRSGKSCRLRWFNQLDPRINRSPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERLM SHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMAR+SR+ SK +   +  T+
Sbjct: 61  EEEEERLMTSHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARKSRDSSKAKAKAKAKTK 120

Query: 121 THN-NNNNNNRLLLSSFLQLHAYPTSNISFI------PKPPSDDAAAAAAHSSCSFIHYY 180
           T     ++ N LL SSFLQ    P +N S        P P S         S    + +Y
Sbjct: 121 TKTLTPSSQNHLLFSSFLQTSCPPNNNASLFLNHSLYPNPISSFIPKPTDRS----VEFY 180

Query: 181 DFLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSASNDDT 229
           DFL +N+ +S+N SEVIDN SRKEDYEEVN          L PFIDFFSA   ++
Sbjct: 181 DFLHLNA-ESINASEVIDN-SRKEDYEEVNQEATATAARPLFPFIDFFSAQTSNS 229

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BLAST of PI0009100 vs. ExPASy TrEMBL
Match: A0A6P3ZUN5 (transcription factor MYB52-like OS=Ziziphus jujuba OX=326968 GN=LOC107419653 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 1.2e-63
Identity = 146/254 (57.48%), Postives = 172/254 (67.72%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MC+RGHWRPAEDEKL+ELVERYGPHNWNAIA+KLQGRSGKSCRLRWFNQLDPRINR PFT
Sbjct: 1   MCTRGHWRPAEDEKLKELVERYGPHNWNAIAEKLQGRSGKSCRLRWFNQLDPRINRNPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSK--TQTLTQTL 120
           EEEEERL+ASHR+HGNRWAIIARLFPGRTDNAVKNHWHVIMARR R +S+   +  +Q L
Sbjct: 61  EEEEERLLASHRIHGNRWAIIARLFPGRTDNAVKNHWHVIMARRCRERSRLYAKRASQKL 120

Query: 121 TQTHNN-------------NNNNNRLLLSSFLQLH----AYPTSNISFIPKPPSDDAAAA 180
             T+               +N   +   +SF++ +     YP  +   +PK  +      
Sbjct: 121 GSTNERKSCLKQAEDVQMVSNCEIKSSFASFIENYRERCKYPFHSTFPLPKQSN-----L 180

Query: 181 AAHSSCSFIH--------YYDFLQVNSTDSMNGSEVIDNSSRKED-YEEVNNNNNGEQQA 226
              S C  IH        +YDFLQVN+    NGSEVIDN+SRK+D  EEVN     EQQ+
Sbjct: 181 LNSSPCFTIHEENSQPKEFYDFLQVNT--ESNGSEVIDNNSRKDDEQEEVNQKEVMEQQS 240

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BLAST of PI0009100 vs. NCBI nr
Match: XP_011649446.1 (transcription factor MYB52 [Cucumis sativus] >KGN63930.1 hypothetical protein Csa_013427 [Cucumis sativus])

HSP 1 Score: 454.1 bits (1167), Expect = 7.0e-124
Identity = 226/232 (97.41%), Postives = 229/232 (98.71%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT
Sbjct: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ
Sbjct: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120

Query: 121 THNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPPSDD-AAAAAAHSSCSFIHYYDFLQVN 180
           THNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPP DD AAAAAAHSSCS IHYYDFLQVN
Sbjct: 121 THNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPPPDDAAAAAAAHSSCSIIHYYDFLQVN 180

Query: 181 STDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSASNDDTSIS 232
           STDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSAS+++TSIS
Sbjct: 181 STDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSASSNNTSIS 232

[Close]

BLAST of PI0009100 vs. NCBI nr
Match: XP_008455801.1 (PREDICTED: transcriptional activator Myb-like [Cucumis melo])

HSP 1 Score: 442.2 bits (1136), Expect = 2.8e-120
Identity = 224/234 (95.73%), Postives = 228/234 (97.44%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINR+PFT
Sbjct: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRSPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ
Sbjct: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120

Query: 121 THNNN-NNNNRLLLSSFLQLHAYPTSNISFIPKPPSDD-AAAAAAHSSCSFIHYYDFLQV 180
           THNNN NNN+RLLLSSFLQLHAYPTS ISF+PKPPSDD AAAAAAHSSCS IHYYDFLQV
Sbjct: 121 THNNNHNNNHRLLLSSFLQLHAYPTSTISFVPKPPSDDAAAAAAAHSSCSIIHYYDFLQV 180

Query: 181 NSTDSMNGSEVIDNSSRKEDYEEVNNNNN-GEQQAVLLPFIDFFSASNDDTSIS 232
           NSTDS NGSEVIDNSSRKEDYEEVNNNNN GEQQAVLLPFIDFFSASN+DTSIS
Sbjct: 181 NSTDSTNGSEVIDNSSRKEDYEEVNNNNNSGEQQAVLLPFIDFFSASNNDTSIS 234

[Close]

BLAST of PI0009100 vs. NCBI nr
Match: XP_038895550.1 (transcription factor MYB54-like [Benincasa hispida])

HSP 1 Score: 353.6 bits (906), Expect = 1.3e-93
Identity = 189/235 (80.43%), Postives = 199/235 (84.68%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT
Sbjct: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERLMASHR+HGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQT T + + 
Sbjct: 61  EEEEERLMASHRLHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTQTPSNSL 120

Query: 121 THNNNN------NNNRLLLSSFLQLHAYPTSNISFIPKPPSDDAAAAAAHSSCSFIHYYD 180
            H+NNN      N+NRLLLSSFLQLH  PTSN  FIPKPPS DAA   A      +HYYD
Sbjct: 121 HHHNNNHHHHHHNHNRLLLSSFLQLHTCPTSNSLFIPKPPSVDAARTNAS-----VHYYD 180

Query: 181 FLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSASNDDTS 230
           FL VN T+S+NGSEVIDN SRKEDYEEV   NNGE Q+V LPFIDFFSAS   T+
Sbjct: 181 FLNVN-TESINGSEVIDN-SRKEDYEEV---NNGEHQSV-LPFIDFFSASQTSTN 224

[Close]

BLAST of PI0009100 vs. NCBI nr
Match: XP_023001003.1 (transcription factor MYB52-like [Cucurbita maxima])

HSP 1 Score: 283.1 bits (723), Expect = 2.1e-72
Identity = 155/246 (63.01%), Postives = 176/246 (71.54%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAED+KLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINR+PFT
Sbjct: 1   MCSRGHWRPAEDDKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRSPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRN----KSKTQTLTQ 120
           EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMAR+SR+    K+K +   +
Sbjct: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARKSRDSSKAKAKAKAKAK 120

Query: 121 TLTQTHN-NNNNNNRLLLSSFLQLHA-------------YPTSNISFIPKPPSDDAAAAA 180
            +T+T   + ++ + LLLSSFLQ                YP    SFIPKP         
Sbjct: 121 AITKTQTLSPSSQSHLLLSSFLQTSCPPNNASLFLNHSLYPNPISSFIPKPTDRS----- 180

Query: 181 AHSSCSFIHYYDFLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFS 229
                  + +YDFL +N+ +S+N SEVIDN SRKEDYEEVN          L PFIDFFS
Sbjct: 181 -------VEFYDFLHLNA-ESINASEVIDN-SRKEDYEEVNQEATATAARPLFPFIDFFS 232

[Close]

BLAST of PI0009100 vs. NCBI nr
Match: XP_023520216.1 (transcription factor MYB52-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 282.7 bits (722), Expect = 2.8e-72
Identity = 153/234 (65.38%), Postives = 172/234 (73.50%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAED+KLRELVERYGPHNWNAIAQKL GRSGKSCRLRWFNQLDPRINR+PFT
Sbjct: 1   MCSRGHWRPAEDDKLRELVERYGPHNWNAIAQKLLGRSGKSCRLRWFNQLDPRINRSPFT 60

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMAR+SR+ SK +   +  T+
Sbjct: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARKSRDSSKAKAKAKAKTK 120

Query: 121 THNNNNNNNRLLLSSFLQLHAYPTSNISFI------PKPPSDDAAAAAAHSSCSFIHYYD 180
           T   ++ N+ LL SSFLQ    P +N S        P P S         S    + +YD
Sbjct: 121 TLTPSSQNH-LLFSSFLQTSCPPNNNASLFLNHSLYPNPISSFIPKPTDRS----VEFYD 180

Query: 181 FLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDFFSASNDDT 229
           FL +N+ +S+N SEVIDN SRKEDYEEVN          L PFIDFFSA   ++
Sbjct: 181 FLHLNA-ESINASEVIDN-SRKEDYEEVNQEATATAARPLFPFIDFFSAQTSNS 227

[Close]

BLAST of PI0009100 vs. TAIR 10
Match: AT1G17950.1 (myb domain protein 52 )

HSP 1 Score: 231.9 bits (590), Expect = 5.3e-61
Identity = 131/247 (53.04%), Postives = 163/247 (65.99%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKLRELVE++GPHNWNAIAQKL GRSGKSCRLRWFNQLDPRINR PFT
Sbjct: 2   MCSRGHWRPAEDEKLRELVEQFGPHNWNAIAQKLSGRSGKSCRLRWFNQLDPRINRNPFT 61

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQ-------- 120
           EEEEERL+ASHR+HGNRW++IAR FPGRTDNAVKNHWHVIMARR R +SK +        
Sbjct: 62  EEEEERLLASHRIHGNRWSVIARFFPGRTDNAVKNHWHVIMARRGRERSKLRPRGLGHDG 121

Query: 121 TLTQT-LTQTHNNNNNNNRLL-------------LSSFLQLHAYPTSNISFIPKPPSDDA 180
           T+  T +   + + +   RL              ++ F  L  + T  I F  +  +   
Sbjct: 122 TVAATGMIGNYKDCDKERRLATTTAINFPYQFSHINHFQVLKEFLTGKIGF--RNSTTPI 181

Query: 181 AAAAAHSSCSFIHYYDFLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFI 226
              A   +   + +Y+FLQVN+   ++  E+IDN SRK++ E+V+ NN    +   +PF 
Sbjct: 182 QEGAIDQTKRPMEFYNFLQVNTDSKIH--ELIDN-SRKDEEEDVDQNNRIPNEN-CVPFF 241

[Close]

BLAST of PI0009100 vs. TAIR 10
Match: AT1G73410.1 (myb domain protein 54 )

HSP 1 Score: 226.9 bits (577), Expect = 1.7e-59
Identity = 128/245 (52.24%), Postives = 159/245 (64.90%), Query Frame = 0


Query: 1   MCSRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFT 60
           MCSRGHWRPAEDEKL++LVE+YGPHNWNAIA KL GRSGKSCRLRWFNQLDPRINR PFT
Sbjct: 3   MCSRGHWRPAEDEKLKDLVEQYGPHNWNAIALKLPGRSGKSCRLRWFNQLDPRINRNPFT 62

Query: 61  EEEEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTLTQ 120
           EEEEERL+A+HR+HGNRW+IIARLFPGRTDNAVKNHWHVIMARR+R  SK + L  T + 
Sbjct: 63  EEEEERLLAAHRIHGNRWSIIARLFPGRTDNAVKNHWHVIMARRTRQTSKPRLLPSTTSS 122

Query: 121 T----------------HNNNNNNNRLLLSSFLQLHAYPTSNISFIPKPPSDDAAAAA-- 180
           +                HN ++++ + +  +      Y  S+I+ +           A  
Sbjct: 123 SSLMASEQIMMSSGGYNHNYSSDDRKKIFPADFINFPYKFSHINHLHFLKEFFTGKIALN 182

Query: 181 --AHSSCSFIHYYDFLQVNSTDSMNGSEVIDNSSRKEDYEEVNNNNNGEQQAVLLPFIDF 226
             A+ S   + +Y+FLQVN TDS N SE+ID  S +    + +  +        +PF DF
Sbjct: 183 HKANQSKKPMEFYNFLQVN-TDS-NKSEIIDQDSGQSKRSDSDTKHESH-----VPFFDF 240

[Close]

BLAST of PI0009100 vs. TAIR 10
Match: AT1G69560.1 (myb domain protein 105 )

HSP 1 Score: 193.7 bits (491), Expect = 1.6e-49
Identity = 88/116 (75.86%), Postives = 101/116 (87.07%), Query Frame = 0


Query: 3   SRGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFTEE 62
           SRGHWRPAED KL+ELV  YGP NWN IA+KLQGRSGKSCRLRWFNQLDPRINR  FTEE
Sbjct: 106 SRGHWRPAEDTKLKELVAVYGPQNWNLIAEKLQGRSGKSCRLRWFNQLDPRINRRAFTEE 165

Query: 63  EEERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKSKTQTLTQTL 119
           EEERLM +HR++GN+WA+IARLFPGRTDN+VKNHWHVIMAR+ R +S +    +T+
Sbjct: 166 EEERLMQAHRLYGNKWAMIARLFPGRTDNSVKNHWHVIMARKFREQSSSYRRRKTM 221

[Close]

BLAST of PI0009100 vs. TAIR 10
Match: AT1G26780.1 (myb domain protein 117 )

HSP 1 Score: 188.7 bits (478), Expect = 5.1e-48
Identity = 95/148 (64.19%), Postives = 108/148 (72.97%), Query Frame = 0


Query: 4   RGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFTEEE 63
           RGHWRPAED KL+ELV  YGP NWN IA+KLQGRSGKSCRLRWFNQLDPRINR  FTEEE
Sbjct: 98  RGHWRPAEDVKLKELVSIYGPQNWNLIAEKLQGRSGKSCRLRWFNQLDPRINRRAFTEEE 157

Query: 64  EERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKS---------KTQTL 123
           EERLM +HR++GN+WA+IARLFPGRTDN+VKNHWHV+MAR+ R  S             L
Sbjct: 158 EERLMQAHRLYGNKWAMIARLFPGRTDNSVKNHWHVVMARKYREHSSAYRRRKLMSNNPL 217

Query: 124 TQTLTQTHNNNNNNNRLLLSSFLQLHAY 143
              LT  H+ N N N     SF+  + Y
Sbjct: 218 KPHLTNNHHPNPNPN---YHSFISTNHY 242

[Close]

BLAST of PI0009100 vs. TAIR 10
Match: AT1G26780.2 (myb domain protein 117 )

HSP 1 Score: 188.7 bits (478), Expect = 5.1e-48
Identity = 95/148 (64.19%), Postives = 108/148 (72.97%), Query Frame = 0


Query: 4   RGHWRPAEDEKLRELVERYGPHNWNAIAQKLQGRSGKSCRLRWFNQLDPRINRTPFTEEE 63
           RGHWRPAED KL+ELV  YGP NWN IA+KLQGRSGKSCRLRWFNQLDPRINR  FTEEE
Sbjct: 98  RGHWRPAEDVKLKELVSIYGPQNWNLIAEKLQGRSGKSCRLRWFNQLDPRINRRAFTEEE 157

Query: 64  EERLMASHRVHGNRWAIIARLFPGRTDNAVKNHWHVIMARRSRNKS---------KTQTL 123
           EERLM +HR++GN+WA+IARLFPGRTDN+VKNHWHV+MAR+ R  S             L
Sbjct: 158 EERLMQAHRLYGNKWAMIARLFPGRTDNSVKNHWHVVMARKYREHSSAYRRRKLMSNNPL 217

Query: 124 TQTLTQTHNNNNNNNRLLLSSFLQLHAY 143
              LT  H+ N N N     SF+  + Y
Sbjct: 218 KPHLTNNHHPNPNPN---YHSFISTNHY 242

The following BLAST results are available for this feature:

BLAST of PI0009100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6R0C4	7.4e-60	53.04	Transcription factor MYB52 OS=Arabidopsis thaliana OX=3702 GN=MYB52 PE=2 SV=1	[more]
Q9FX36	2.4e-58	52.24	Transcription factor MYB54 OS=Arabidopsis thaliana OX=3702 GN=MYB54 PE=1 SV=1	[more]
Q5NBM8	5.3e-50	79.82	Transcription factor CSA OS=Oryza sativa subsp. japonica OX=39947 GN=CSA PE=2 SV...	[more]
Q9SEZ4	2.2e-48	75.86	Transcription factor MYB105 OS=Arabidopsis thaliana OX=3702 GN=MYB105 PE=1 SV=1	[more]
Q9LQX5	7.2e-47	64.19	Transcription factor MYB117 OS=Arabidopsis thaliana OX=3702 GN=MYB117 PE=2 SV=1	[more]

BLAST of PI0009100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPV0	3.4e-124	97.41	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G029570 PE=4 SV=1	[more]
A0A1S3C1Q2	1.3e-120	95.73	transcriptional activator Myb-like OS=Cucumis melo OX=3656 GN=LOC103495663 PE=4 ...	[more]
A0A6J1KP91	1.0e-72	63.01	transcription factor MYB52-like OS=Cucurbita maxima OX=3661 GN=LOC111495269 PE=4...	[more]
A0A6J1EDT2	3.0e-72	64.68	transcription factor MYB52-like OS=Cucurbita moschata OX=3662 GN=LOC111431625 PE...	[more]
A0A6P3ZUN5	1.2e-63	57.48	transcription factor MYB52-like OS=Ziziphus jujuba OX=326968 GN=LOC107419653 PE=...	[more]

BLAST of PI0009100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011649446.1	7.0e-124	97.41	transcription factor MYB52 [Cucumis sativus] >KGN63930.1 hypothetical protein Cs...	[more]
XP_008455801.1	2.8e-120	95.73	PREDICTED: transcriptional activator Myb-like [Cucumis melo]	[more]
XP_038895550.1	1.3e-93	80.43	transcription factor MYB54-like [Benincasa hispida]	[more]
XP_023001003.1	2.1e-72	63.01	transcription factor MYB52-like [Cucurbita maxima]	[more]
XP_023520216.1	2.8e-72	65.38	transcription factor MYB52-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0009100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17950.1	5.3e-61	53.04	myb domain protein 52	[more]
AT1G73410.1	1.7e-59	52.24	myb domain protein 54	[more]
AT1G69560.1	1.6e-49	75.86	myb domain protein 105	[more]
AT1G26780.1	5.1e-48	64.19	myb domain protein 117	[more]
AT1G26780.2	5.1e-48	64.19	myb domain protein 117	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001005	SANT/Myb domain	SMART	SM00717	sant	coord: 55..103 e-value: 5.4E-14 score: 62.5 coord: 3..52 e-value: 3.6E-15 score: 66.4
IPR001005	SANT/Myb domain	PROSITE	PS50090	MYB_LIKE	coord: 1..50 score: 11.230598
IPR001005	SANT/Myb domain	PROSITE	PS50090	MYB_LIKE	coord: 51..101 score: 9.697762
IPR001005	SANT/Myb domain	CDD	cd00167	SANT	coord: 58..98 e-value: 1.2874E-10 score: 52.963
IPR001005	SANT/Myb domain	CDD	cd00167	SANT	coord: 7..48 e-value: 4.07237E-14 score: 62.593
None	No IPR available	PFAM	PF13921	Myb_DNA-bind_6	coord: 7..67 e-value: 8.8E-17 score: 61.1
None	No IPR available	GENE3D	1.10.10.60		coord: 57..132 e-value: 4.7E-19 score: 70.2
None	No IPR available	GENE3D	1.10.10.60		coord: 4..55 e-value: 1.7E-24 score: 87.5
None	No IPR available	PANTHER	PTHR45614:SF111	MYB-RELATED PROTEIN A-LIKE	coord: 1..223
None	No IPR available	PANTHER	PTHR45614	MYB PROTEIN-RELATED	coord: 1..223
IPR017930	Myb domain	PROSITE	PS51294	HTH_MYB	coord: 1..54 score: 25.942102
IPR017930	Myb domain	PROSITE	PS51294	HTH_MYB	coord: 55..105 score: 22.804882
IPR009057	Homeobox-like domain superfamily	SUPERFAMILY	46689	Homeodomain-like	coord: 4..97

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0009100.1	PI0009100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding