PI0009077 (gene) Melon (PI 482460) v1

Overview
NamePI0009077
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionprotein NRT1/ PTR FAMILY 3.1
Locationchr03: 16846022 .. 16851144 (-)
RNA-Seq ExpressionPI0009077
SyntenyPI0009077
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATGGAGAAAAAGGAAAAGCATATGGAAAGGAAGAAAGGTGGCCTTATTACTATGCCATTCATATTCGGTACTCTTTCTTCTCTTCTTCTTCTTCCTTCTAGATCTGTTTTTTTTTTTTGTTTTATTTTATTTTTTTCTTTTTCCTAATTAAACTTTTTTGTGAATAATAAGCAAATGAGATTTGCGAGAAGATTGCTGTGGTTGGTTTTAGTACCAACATGATTAGCTATTTGACAAAGCAACTTCATATGCCTTCAACTAAAGCAGCCAATACCATCACTAATTTCAGTGGAACCGCCAGCTTGACACCCCTCATCGGAGCTTTCATCGCCGATTCTTACGTCGGCCGTTTCTGGACCATCACCGTCGCCTCTATCCTTTACCAGATTGTATATTATTTCTACTACTCCATACCAATTTCTTTAACTTTTTATTACTTTTATATAATACTCCAAAAAAATGCCCATACTCTCATCTCTGTTTTTTTAGCATAACAAATAAAGTAGATGAGATTTTCAATATCTTAACTTCAAAACAAATAGTAGATACTAATTATCCATGAGCTAGGCATGTTCAATTTGGTAAAGAACACATAAATTAGAGCAAAAATTTGTATAATCTCAACCTCTTAGGACAATAATTAAATGCAACATAGTATGATAGGAAGAGAAAAGAGATTATAGAATCTTTTTAATATCTTTTTAATCATAATAATTAAGTACAAAAAGATTCCTCCCTTTTCAAATTCAACAAAATATATTAATGATCAAAGTATCTCATCTTTGATCATTAATATTTTAGATGAGGAATATGAGGAATCAAACAACGTTAGATAAGAAGGGGAAAAAATTAATTATTAGTGGAATAATAATAATTAATTAAGCATATTATTAATAACTTTAATATTGGGGTTATTATAGGGGATGATAAGCCTAACAACATCAGCAGTGGTGCCGGGGCTGAGGCCACCACCATGCAATGGCGGAGAAATATGCAAGGAAGCCAGCGGAGGACAACTAGGAATACTATACGTATCTCTACTCTTGACAGCGCTTGGGTCTGGGGGAATCCGACCGTGCGTGGTGTCGTTCGGAGCAGACCAATTCGAGGAGGGAGATCCGAAACGTGGGACAAAGACGTACAAATACTTCAATTGGTATTATTTCGCGATGGGGGCGTCGATGCTGGTGGCGGTGACGGTTCTTGTGTATGTGCAAGATAACATTGGATGGGGATGGGGATTTGGAATTCCTACCATTGCAATGTTGGTGTCCATTGTTACATTTGTTGTGGGTACCCGATTTACAGGCATTTGGATCCGTCGGGTAGCCCGTTTACCCGTTTATTGCAAGTAGGAGTTGCGGCTTATAGGAAGAGGAAAGTTTGTACTGTCTCTGATTCCAATTTGCTTTATCAGAATCATGATATCGATGACCCTATTTCTCGAGATGGCAAGCTTGTTCATACTAAACATATGAGGTATTTCATCTCTCTCTACACTACCATTTTACTTTTACTTTTACTTTTTAGTTAATACCACTTTTTGTCACCTGGTTCAATAATCACTTTATAATAGGTCTTTTAATTATTAAAAATCAATTTATACCTTTATTTATTTCATAATTACTAATATCAAACATATATATGTCCTCTTTTGGGTGGTACTTCACTCATCTTTTTTCACAAGACCAAAACTTAGTTTTCAAATTACAAGTTTAGTATCCCAACATAATTGTGTTTAAAAGGTATGTAAAGAGTTATTTAGATCCTTGAGTTGGTTATATTAATTTGTATTTGGATACATATATACTATTTTGATTTTAGTAGTATTATAATAGTTTGTGTTTGCGGTGTCCGTTATTTAGGTCTAGGTTAAAAATAAAGATTATTATTACAAAATATAGTAAAAAGTAAGTGGAAGATGATGACTTTTTAATAGACTTATTGTAACAAATGTGTTATAATAATTAGAAACTCTAACTGTTATATATATAAGGTGTAACTCAAGCATTAGGTAGACTAATATATAGGTCACACTTGAGGTCAAAACAGTCCCTAATTTTTTATTTTTGTAGTTAATGTAGGAGTTGATGGGTCCACCACGGAAATATATCAATTTTATATCTTATCAATTTTTTACACGATAAATCTAAAAAGCCTACCTTCTAACGCTTTGTCAAAAGCCCCCTAAAACGTTTGATGTTTTTATTCAAACAAGTAGGAAGATTGTTTTTCCTTTTTATAATATTATATAAATTTTAAAGTAAATAGTAAATGATTTGGTAGATTAATGAAAATGTAGTGTTTGAAAGAAAAAAGATAGAATTGATTTTGTCTTTGAGTGGCGAGGGGATGCAAATATGAGTCAGAATCTGATTAACTTTGCCCCTCTGCTTCACAAACAACTCACTACAATTTTCATTTCTTTTGTCTTTTTTGCTAAACAACAAAACCACCTCTGTTTTCCAATTCTTATCTTTCCTTTTTATTTTTTTTTCAAATGCCCATCAATTTTAAATAAATTAATTAAACTAGGCCCCTCATTGTTTTTCATTTTTCCACAATTATCTCTTTCTTAATTTCTACCCTCCATAAGCATATACATATTGTTTCTTAGTTTATATATACAATTGCTCCCACCCATAACTTTTACGATTCTAATTTTTCTTTTACACTTGTTGAATGTTGTTAACTATGATTTAGATAGGTGAAATTGAAATAATTGAAGGTTAGATATAATTTTAGTAATTCAAAATTTTAGTCTCTTTAAGATTCTCCTTTGATAATTTTTTAATTTTTCATTTTTATTTTTAAAAATTAAATATAAACTTTTATTATCTATTTTATAGAATTAATTTTCAATATTCAAGTCACGTTTTAAAAACCTTAAAAATGTAAATATAATTTTAAAATTTTACTTTTTATTTTCAAATTCAGTTCACATGTCTAGTTAAGTGAAAACTATTCTTAGAGAAGTTGTAAGAAAACAACAAAAGAAAATAATAAACCCAACAAGAATCAAATAGAACGTTTTAAATCCAATTTTTGTTTATTTAATTTAAAATAATACTCAAAGCTTTTACATTTATGTCTAGTTTCTTAAAAAACTTGGAATCAATCAAAAGCTCAAGAAACAAAAAAATAATAATAATAATCTTATCAACACAAAATTCAAAATTTTCAAATTTATTAGACACCTTTTAAGATGCTCTATCAAACACGATTAAAAAGTCATCAACTTATCATACTAAACAACTAAAGTTGTAGTGTAACTATAAATAATAAATAAAAGAAAAGAAAAGAAAATATTTTGAAAGAACAAAGAGCATTACCTAAAATAGTAAAGTTGGATCTAGTGTTAAAAGCACAATAACTAACAAAGTAAGTAGGCACAAGGAAACACAAACAAATTGTTAACCCAGTTCAATGCAACATCACCTCTGAGGAACTATGCCCAATTAAGAAGAAATATTCACACAAAAGTTTAAGAGAATTACAAGTATACGACTTATCTGTTAAATGTACTATCTTGAATTACAATATAAGACCACATTAGAAGATTGTTTAGATTCTCCCTAAGTGTGTTGATATTTGATTCACCCTCTTTTTAGGATTCAAGCTCCCCTTTGAACACGTTAACTCTAGCAATAATATAGTAAAGTTAGGTTGCCCTAACTACTATACTACATCTTCTTAATAATGTTGAACATCACGTCAAACTCCAACAAGCATCACAACCAAGATTACTGATTTTCTTCAACGTCATACCAACTTTTCTAACCAAACAGTATGCAACAACACAACTAGGTTATATTTTTCACACAAATGGCACAAGGCCGCAAGACAACTTTTATATTCAACCTTAAAAAGGAAAAAGACCAAAAACAACAATTAGGACCTCAACAAAAACAACGGGAAAATCCCACAATAATGGGGAGACAACAAAAGGAAAGTCCCTACAAGGAAACCACAAAACAAACCAACACCACCAAAACAGTAACAAATTCTATTTACTATCATCCAACCAAACAAAAAACTGTCTAGTACAGTCATCACAATGCAATAATATTTTTAAATATAGATACACTAAATGTAGACTTAAAAACTTGTATATGATTTTATTAAGGTACTTTTGTCATTTTCTGTAATACGTCTATGAATATAAAATTATCAATCACACATTGTAACGATACGTAATCGGATTACGATGCAGATTTCTAGACAAGGCAGCCATAGTTACAGAAGAAGAGAAGGTAAAAGTGGGAGAAGAATTAGAAAAACCCAATCTATGGAAACTAAGCACAGTCCATAGAGTTGAAGAGCTAAAATCCGTGATAAGAATGGGTCCAATCTGGGCCTCAGGAATCATCTTAATCACAGCATATTCTCAACAGAACACATTTTCTCTCCTTCAAGCTTCAACAATGAACAGACACTTAACAAACTCATTCCAAATCCCAGCTGGTTCCATGACTGTCTTCACCCTCCTCACAATGCTTACCACCGTCGCCTTATATGATCGTGTCTTCGTCCCCATCGCCCGTAAATTCACTGGCCTCGACCGTGGCATCACCTTCCTCACTCGCATGGGCATCGGCCTTGTCATCTCCATCTTCGCCACATTCATAGCCGGCTTCGCCGAGCAAAAGCGCAAGCACGTTGCTTTCACTCATGGCCTCATGGATCACCCTACTTCCATCATTCCCATCTCCGTGTTCTGGCTCGTCCCGCAGTATAGCCTGCATGGGATGGCTGAGGCGTTCATGTCTATTGGGCATCTTGAATTCTTCTATGATCAGAGTCCTGAGAGTATGAGGAGCACTGCCATGGCTTTGTTTTGGACTTCAATTTCAGCTGGAAATTATTTGAGTACTATGTTGGTCAGTTTGGTTGAAAAGTATAGTGGAGGAGCTAATGGGGAGAATTGGATTAGGAATGATAATATTAACAAGGGGAAGTTGGAGAATTTTTATTGGTTGATTACTCTTTTGCAAGTTTTAAATCTTGTTTATTACATTGTTTGTGCTAAGTTTTATACTTTTAAGCCTTTGGAGGTTCAGAGGAAAGTTGTGGATAGCTCTAAGGTGGATGAACTTCAACTTGTCAACCCTGTTTAG

mRNA sequence

ATGGAGATGGAGAAAAAGGAAAAGCATATGGAAAGGAAGAAAGGTGGCCTTATTACTATGCCATTCATATTCGCAAATGAGATTTGCGAGAAGATTGCTGTGGTTGGTTTTAGTACCAACATGATTAGCTATTTGACAAAGCAACTTCATATGCCTTCAACTAAAGCAGCCAATACCATCACTAATTTCAGTGGAACCGCCAGCTTGACACCCCTCATCGGAGCTTTCATCGCCGATTCTTACGTCGGCCGTTTCTGGACCATCACCGTCGCCTCTATCCTTTACCAGATTGGGATGATAAGCCTAACAACATCAGCAGTGGTGCCGGGGCTGAGGCCACCACCATGCAATGGCGGAGAAATATGCAAGGAAGCCAGCGGAGGACAACTAGGAATACTATACGTATCTCTACTCTTGACAGCGCTTGGGTCTGGGGGAATCCGACCGTGCGTGGTGTCGTTCGGAGCAGACCAATTCGAGGAGGGAGATCCGAAACGTGGGACAAAGACGTACAAATACTTCAATTGGTATTATTTCGCGATGGGGGCGTCGATGCTGGTGGCGGTGACGGTTCTTGTGTATGTGCAAGATAACATTGGATGGGGATGGGGATTTGGAATTCCTACCATTGCAATGTTGGTGCATTTGGATCCGTCGGGTAGCCCGTTTACCCGTTTATTGCAAGTAGGAGTTGCGGCTTATAGGAAGAGGAAAGTTTGTACTGTCTCTGATTCCAATTTGCTTTATCAGAATCATGATATCGATGACCCTATTTCTCGAGATGGCAAGCTTGTTCATACTAAACATATGAGATTTCTAGACAAGGCAGCCATAGTTACAGAAGAAGAGAAGGTAAAAGTGGGAGAAGAATTAGAAAAACCCAATCTATGGAAACTAAGCACAGTCCATAGAGTTGAAGAGCTAAAATCCGTGATAAGAATGGGTCCAATCTGGGCCTCAGGAATCATCTTAATCACAGCATATTCTCAACAGAACACATTTTCTCTCCTTCAAGCTTCAACAATGAACAGACACTTAACAAACTCATTCCAAATCCCAGCTGGTTCCATGACTGTCTTCACCCTCCTCACAATGCTTACCACCGTCGCCTTATATGATCGTGTCTTCGTCCCCATCGCCCGTAAATTCACTGGCCTCGACCGTGGCATCACCTTCCTCACTCGCATGGGCATCGGCCTTGTCATCTCCATCTTCGCCACATTCATAGCCGGCTTCGCCGAGCAAAAGCGCAAGCACGTTGCTTTCACTCATGGCCTCATGGATCACCCTACTTCCATCATTCCCATCTCCGTGTTCTGGCTCGTCCCGCAGTATAGCCTGCATGGGATGGCTGAGGCGTTCATGTCTATTGGGCATCTTGAATTCTTCTATGATCAGAGTCCTGAGAGTATGAGGAGCACTGCCATGGCTTTGTTTTGGACTTCAATTTCAGCTGGAAATTATTTGAGTACTATGTTGGTCAGTTTGGTTGAAAAGTATAGTGGAGGAGCTAATGGGGAGAATTGGATTAGGAATGATAATATTAACAAGGGGAAGTTGGAGAATTTTTATTGGTTGATTACTCTTTTGCAAGTTTTAAATCTTGTTTATTACATTGTTTGTGCTAAGTTTTATACTTTTAAGCCTTTGGAGGTTCAGAGGAAAGTTGTGGATAGCTCTAAGGTGGATGAACTTCAACTTGTCAACCCTGTTTAG

Coding sequence (CDS)

ATGGAGATGGAGAAAAAGGAAAAGCATATGGAAAGGAAGAAAGGTGGCCTTATTACTATGCCATTCATATTCGCAAATGAGATTTGCGAGAAGATTGCTGTGGTTGGTTTTAGTACCAACATGATTAGCTATTTGACAAAGCAACTTCATATGCCTTCAACTAAAGCAGCCAATACCATCACTAATTTCAGTGGAACCGCCAGCTTGACACCCCTCATCGGAGCTTTCATCGCCGATTCTTACGTCGGCCGTTTCTGGACCATCACCGTCGCCTCTATCCTTTACCAGATTGGGATGATAAGCCTAACAACATCAGCAGTGGTGCCGGGGCTGAGGCCACCACCATGCAATGGCGGAGAAATATGCAAGGAAGCCAGCGGAGGACAACTAGGAATACTATACGTATCTCTACTCTTGACAGCGCTTGGGTCTGGGGGAATCCGACCGTGCGTGGTGTCGTTCGGAGCAGACCAATTCGAGGAGGGAGATCCGAAACGTGGGACAAAGACGTACAAATACTTCAATTGGTATTATTTCGCGATGGGGGCGTCGATGCTGGTGGCGGTGACGGTTCTTGTGTATGTGCAAGATAACATTGGATGGGGATGGGGATTTGGAATTCCTACCATTGCAATGTTGGTGCATTTGGATCCGTCGGGTAGCCCGTTTACCCGTTTATTGCAAGTAGGAGTTGCGGCTTATAGGAAGAGGAAAGTTTGTACTGTCTCTGATTCCAATTTGCTTTATCAGAATCATGATATCGATGACCCTATTTCTCGAGATGGCAAGCTTGTTCATACTAAACATATGAGATTTCTAGACAAGGCAGCCATAGTTACAGAAGAAGAGAAGGTAAAAGTGGGAGAAGAATTAGAAAAACCCAATCTATGGAAACTAAGCACAGTCCATAGAGTTGAAGAGCTAAAATCCGTGATAAGAATGGGTCCAATCTGGGCCTCAGGAATCATCTTAATCACAGCATATTCTCAACAGAACACATTTTCTCTCCTTCAAGCTTCAACAATGAACAGACACTTAACAAACTCATTCCAAATCCCAGCTGGTTCCATGACTGTCTTCACCCTCCTCACAATGCTTACCACCGTCGCCTTATATGATCGTGTCTTCGTCCCCATCGCCCGTAAATTCACTGGCCTCGACCGTGGCATCACCTTCCTCACTCGCATGGGCATCGGCCTTGTCATCTCCATCTTCGCCACATTCATAGCCGGCTTCGCCGAGCAAAAGCGCAAGCACGTTGCTTTCACTCATGGCCTCATGGATCACCCTACTTCCATCATTCCCATCTCCGTGTTCTGGCTCGTCCCGCAGTATAGCCTGCATGGGATGGCTGAGGCGTTCATGTCTATTGGGCATCTTGAATTCTTCTATGATCAGAGTCCTGAGAGTATGAGGAGCACTGCCATGGCTTTGTTTTGGACTTCAATTTCAGCTGGAAATTATTTGAGTACTATGTTGGTCAGTTTGGTTGAAAAGTATAGTGGAGGAGCTAATGGGGAGAATTGGATTAGGAATGATAATATTAACAAGGGGAAGTTGGAGAATTTTTATTGGTTGATTACTCTTTTGCAAGTTTTAAATCTTGTTTATTACATTGTTTGTGCTAAGTTTTATACTTTTAAGCCTTTGGAGGTTCAGAGGAAAGTTGTGGATAGCTCTAAGGTGGATGAACTTCAACTTGTCAACCCTGTTTAG

Protein sequence

MEMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTITNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEICKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLVHLDPSGSPFTRLLQVGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVGEELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTNSFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATFIAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLITLLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV
Homology
BLAST of PI0009077 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 9.0e-232
Identity = 405/582 (69.59%), Postives = 474/582 (81.44%), Query Frame = 0

Query: 4   EKKEKH--MERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTIT 63
           E+K+ H    R KGGLITMPFIFANEICEK+AVVGF  NMISYLT QLH+P TKAANT+T
Sbjct: 12  EEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLT 71

Query: 64  NFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEI 123
           NF+GT+SLTPL+GAFIADS+ GRFWTIT ASI+YQIGM  LT SA++P LRPPPC G E+
Sbjct: 72  NFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV 131

Query: 124 CKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAM 183
           C  A   QL ILYV+LLL ALGSGGIRPCVV+FGADQF+E DP + TKT+ YFNWYYF M
Sbjct: 132 CVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCM 191

Query: 184 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLLQ 243
           GA++L+AVTVLV++QDN+GWG G GIPT+AM +             HL P+GSPFTRL+Q
Sbjct: 192 GAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQ 251

Query: 244 VGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVG 303
           VGVAA+RKRK+  VSD +LLY N +ID PIS  GKL HTKHM FLDKAAIVTEE+ +K G
Sbjct: 252 VGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVTEEDNLKPG 311

Query: 304 EELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTN 363
              + PN W+LSTVHRVEELKSVIRMGPI ASGI+LITAY+QQ TFSL QA TMNRHLTN
Sbjct: 312 ---QIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTN 371

Query: 364 SFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATF 423
           SFQIPAGSM+VFT + MLTT+  YDRVFV +ARKFTGL+RGITFL RMGIG VISI AT 
Sbjct: 372 SFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATL 431

Query: 424 IAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 483
           +AGF E KRK VA  HGL+D P +I+PIS  WL+PQY LHG+AEAFMSIGHLEFFYDQ+P
Sbjct: 432 VAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAP 491

Query: 484 ESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLIT 543
           ESMRSTA ALFW +IS GNY+ST+LV+LV K+S   +G NW+ ++N+N+G+LE FYWLIT
Sbjct: 492 ESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLIT 551

Query: 544 LLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKV-DELQLVN 570
           +LQ +NLVYY+ CAK YT+KP++V     DSS V +ELQL N
Sbjct: 552 VLQAVNLVYYLWCAKIYTYKPVQVHHSKEDSSPVKEELQLSN 590

BLAST of PI0009077 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 6.0e-135
Identity = 260/561 (46.35%), Postives = 362/561 (64.53%), Query Frame = 0

Query: 1   MEMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTI 60
           +++ KK  + + K G      FI   E CE++A  G STN+I+YL KQ++M +  A+ ++
Sbjct: 14  LDIHKKPAN-KNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSV 73

Query: 61  TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGE 120
           +N+SGT   TPLIGAFIAD+Y+GR+WTI    ++Y  GM  LT SA VPGL  P C+ GE
Sbjct: 74  SNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTCS-GE 133

Query: 121 ICKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA 180
            C  A+ GQ  I +++L L ALG+GGI+PCV SFGADQF++ D K       +FNW+YF 
Sbjct: 134 TC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFV 193

Query: 181 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLVHL-------------DPSGSPFTRLL 240
           +    ++A +VLV++Q N+GWGWG G+PT+AM + +              P GSP TR+L
Sbjct: 194 INVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRML 253

Query: 241 QVGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKV 300
           QV VA+ RK KV    D +LLY+N D +  I    KL HTK + F DKAA+ TE +    
Sbjct: 254 QVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESD---- 313

Query: 301 GEELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLT 360
            +   K + WKL TV +VEELK++IR+ PIWA+GI+  + YSQ  T  +LQ +T+++H+ 
Sbjct: 314 NKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMG 373

Query: 361 NSFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFAT 420
            +F+IP+ S+++F  L++L    +YD++ VP ARK+TG +RG T L R+GIGLVISIF+ 
Sbjct: 374 PNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSM 433

Query: 421 FIAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 480
             AG  E  R +   TH L +  T  IP+++FW VPQY L G AE F  IG LEFFYDQ+
Sbjct: 434 VSAGILEVARLNYVQTHNLYNEET--IPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQA 493

Query: 481 PESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLI 540
           P++MRS   AL  T+I+ GNYLST LV+LV K +       WI   N+N G L+ F+WL+
Sbjct: 494 PDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAK-NLNNGHLDYFFWLL 553

Query: 541 TLLQVLNLVYYIVCAKFYTFK 549
             L  LN + Y+  AK+YT+K
Sbjct: 554 AGLSFLNFLVYLWIAKWYTYK 563

BLAST of PI0009077 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 8.6e-134
Identity = 248/551 (45.01%), Postives = 353/551 (64.07%), Query Frame = 0

Query: 11  ERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTITNFSGTASLT 70
           + K G      FI  NE CE++A  G  TN+++YL  +L+  +  AAN +TN+SGT  +T
Sbjct: 22  KEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYIT 81

Query: 71  PLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEICKEASGGQL 130
           PLIGAFIAD+Y+GR+WTI     +Y  GM  LT SA VPGL+P  CN  + C   +  Q 
Sbjct: 82  PLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN-ADTC-HPNSSQT 141

Query: 131 GILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAVT 190
            + +V+L + ALG+GGI+PCV SFGADQF+E D     K   +FNW+YF++    L+A T
Sbjct: 142 AVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAAT 201

Query: 191 VLVYVQDNIGWGWGFGIPTIAMLVHL-------------DPSGSPFTRLLQVGVAAYRKR 250
           VLV++Q N+GWGWGFG+PT+AM++ +              P GSP TR+ QV VAA+RK 
Sbjct: 202 VLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKI 261

Query: 251 KVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVGEELEKPNLW 310
            V    D +LL++  D +  I    KLVHT +++F DKAA+ ++ + +K GE     N W
Sbjct: 262 SVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGE----VNPW 321

Query: 311 KLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTNSFQIPAGSM 370
           +L +V +VEELKS+I + P+WA+GI+  T YSQ +T  +LQ +TM++H+  +F+IP+ S+
Sbjct: 322 RLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSASL 381

Query: 371 TVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATFIAGFAEQKR 430
           ++F  +++L    +YD+  +P+ARKFT  +RG T L RMGIGLV+SIFA   AG  E  R
Sbjct: 382 SLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVR 441

Query: 431 KHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMA 490
                TH   D     I +S+FW +PQY L G AE F  IG LEFFYDQ+P++MRS   A
Sbjct: 442 LDYVKTHNAYDQKQ--IHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSA 501

Query: 491 LFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLITLLQVLNLVY 549
           L  T+++ GNYLST+LV++V K +       WI  DN+N+G L+ F++L+  L  LN + 
Sbjct: 502 LSLTTVALGNYLSTVLVTVVMKITKKNGKPGWI-PDNLNRGHLDYFFYLLATLSFLNFLV 561

BLAST of PI0009077 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.4e-123
Identity = 242/552 (43.84%), Postives = 340/552 (61.59%), Query Frame = 0

Query: 10  MERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTITNFSGTASL 69
           ++ K G     PFI  NE CE++A  G + N+I+YLT +LH  +  AA  +T + GT  L
Sbjct: 38  LKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYL 97

Query: 70  TPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEICKEASGGQ 129
           TPLIGA +AD+Y GR+WTI   S +Y IGM +LT SA VP L+P  C  G+ C  A+  Q
Sbjct: 98  TPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECI-GDFCPSATPAQ 157

Query: 130 LGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAV 189
             + +  L L ALG+GGI+PCV SFGADQF++ D +   +   +FNW+YF++    LV+ 
Sbjct: 158 YAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSS 217

Query: 190 TVLVYVQDNIGWGWGFGIPTIAMLVHL-------------DPSGSPFTRLLQVGVAAYRK 249
           ++LV++Q+N GWG GFGIPT+ M + +              P GSP TR+ QV VA++RK
Sbjct: 218 SLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRK 277

Query: 250 RKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVGEELEKPNL 309
             V    D+ LLY+  D +  I+   K+ HT   ++LDKAA+++EEE  K G   +  N 
Sbjct: 278 SSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEEES-KSG---DYSNS 337

Query: 310 WKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTNSFQIPAGS 369
           W+L TV +VEELK +IRM PIWASGII    Y+Q +T  + Q   MN  +  SFQ+P  +
Sbjct: 338 WRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPAA 397

Query: 370 MTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATFIAGFAEQK 429
           +  F   +++  V LYDR  VP+ARKFTG+D+G T + RMGIGL +S+     A   E  
Sbjct: 398 LGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEII 457

Query: 430 RKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAM 489
           R H+A   GL++     +PISV W +PQY + G AE F  IG LEFFYDQSP++MRS   
Sbjct: 458 RLHMANDLGLVESGAP-VPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCS 517

Query: 490 ALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLITLLQVLNLV 549
           AL   + + GNYLS+++++LV  ++     E WI +DN+N G L+ F+WL+  L ++N+ 
Sbjct: 518 ALALLTNALGNYLSSLILTLVTYFTTRNGQEGWI-SDNLNSGHLDYFFWLLAGLSLVNMA 577

BLAST of PI0009077 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 3.5e-119
Identity = 235/556 (42.27%), Postives = 339/556 (60.97%), Query Frame = 0

Query: 11  ERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTITNFSGTASLT 70
           ++K G     PFI  NE CE++A  G + N+I+Y T +LH  +  AA+ +  + GT  +T
Sbjct: 50  KKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYIT 109

Query: 71  PLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNG--GEICKEASGG 130
           PLIGA IADSY GR+WTI   S +Y IGM  LT SA +P L+P  C G    +C  A+  
Sbjct: 110 PLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSPATTV 169

Query: 131 QLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVA 190
           Q  + +  L L ALG+GGI+PCV SFGADQF++ DP+   +   +FNW+YF++     ++
Sbjct: 170 QYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGSFIS 229

Query: 191 VTVLVYVQDNIGWGWGFGIPTIAMLVHL-------------DPSGSPFTRLLQVGVAAYR 250
            T+LV+VQ+N+GWG GF IPT+ M V +              P GSP TR+ QV VAAYR
Sbjct: 230 STLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVAAYR 289

Query: 251 KRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVGEELEKPN 310
           K K+    D + LY+  + +  I+   K+ HT   +FLDKAA+++E E  K G      N
Sbjct: 290 KLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYES-KSG---AFSN 349

Query: 311 LWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTNSFQIPAG 370
            WKL TV +VEE+K++IRM PIWASGI+    YSQ +T  + Q  +MNR +  SF+IP  
Sbjct: 350 PWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNR-IIRSFEIPPA 409

Query: 371 SMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATFIAGFAEQ 430
           S  VF  L +L ++ +YDR  VP  R+FTG+ +G+T L RMGIGL +S+ +   A   E 
Sbjct: 410 SFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVET 469

Query: 431 KRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTA 490
            R  +A            + +S+FW +PQY L G+AE F  IG +EFFYD+SP++MRS  
Sbjct: 470 VRLQLA---------QDFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVC 529

Query: 491 MALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLITLLQVLNL 550
            AL   + + G+YLS+++++LV  ++     + W+  D++NKG L+ F+WL+  L ++N+
Sbjct: 530 SALALLNTAVGSYLSSLILTLVAYFTALGGKDGWV-PDDLNKGHLDYFFWLLVSLGLVNI 589

BLAST of PI0009077 vs. ExPASy TrEMBL
Match: A0A1S3BD88 (protein NRT1/ PTR FAMILY 3.1 OS=Cucumis melo OX=3656 GN=LOC103488612 PE=3 SV=1)

HSP 1 Score: 1069.7 bits (2765), Expect = 4.2e-309
Identity = 541/584 (92.64%), Postives = 555/584 (95.03%), Query Frame = 0

Query: 1   MEMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTI 60
           MEMEK EKHMERKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMPSTKAANTI
Sbjct: 1   MEMEKTEKHMERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPSTKAANTI 60

Query: 61  TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGE 120
           TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPC  GE
Sbjct: 61  TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKAGE 120

Query: 121 ICKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA 180
           ICKEA+ GQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA
Sbjct: 121 ICKEATEGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA 180

Query: 181 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLL 240
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV             HLDPSGSPF+RLL
Sbjct: 181 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLVSIITFVVGYPIYRHLDPSGSPFSRLL 240

Query: 241 QVGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKV 300
           QVGVAAYRKRKV +VSDSNLLYQN DID PISRDG L+HTKHMRFLDKAA+VTEEEKVK+
Sbjct: 241 QVGVAAYRKRKVGSVSDSNLLYQNRDIDGPISRDGVLLHTKHMRFLDKAAVVTEEEKVKM 300

Query: 301 GEELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLT 360
           GEEL KPNLWKLSTVHRVEELKSVIRMGPIWA+GIILITAYSQQNTFSLLQASTMNRHLT
Sbjct: 301 GEELGKPNLWKLSTVHRVEELKSVIRMGPIWAAGIILITAYSQQNTFSLLQASTMNRHLT 360

Query: 361 NSFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFAT 420
           NSFQIPAGSMTVFTLLTML TVALYDRVF+PIARKFTGLDRGITFLTRMGIGLVISIFAT
Sbjct: 361 NSFQIPAGSMTVFTLLTMLITVALYDRVFIPIARKFTGLDRGITFLTRMGIGLVISIFAT 420

Query: 421 FIAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 480
            IAGF EQKRKHVAF HGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 421 LIAGFVEQKRKHVAFNHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 480

Query: 481 PESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLI 540
           PESMRSTAMALFWTSISAG+YLST+LVSLV KYSGGANGENWIRNDNINKGKLENFYWLI
Sbjct: 481 PESMRSTAMALFWTSISAGSYLSTLLVSLVHKYSGGANGENWIRNDNINKGKLENFYWLI 540

Query: 541 TLLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 572
           T+LQVLNL YYIVCA+FYTFKPLEVQRKV+D SKVDELQLVNPV
Sbjct: 541 TVLQVLNLAYYIVCARFYTFKPLEVQRKVMDGSKVDELQLVNPV 584

BLAST of PI0009077 vs. ExPASy TrEMBL
Match: A0A0A0K953 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G037500 PE=3 SV=1)

HSP 1 Score: 1032.7 bits (2669), Expect = 5.7e-298
Identity = 521/567 (91.89%), Postives = 535/567 (94.36%), Query Frame = 0

Query: 18  ITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTITNFSGTASLTPLIGAFI 77
           +T     +NEICEKIAVVGF+TNMISYLTKQLHMPSTKAANTITNFSGTASLTPLIGAFI
Sbjct: 4   VTPSLSLSNEICEKIAVVGFNTNMISYLTKQLHMPSTKAANTITNFSGTASLTPLIGAFI 63

Query: 78  ADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEICKEASGGQLGILYVSL 137
           ADSYVGRFWTITVASILYQIGM+SLTTSAVVPGLRPPPC  GEICKEA+GGQLGILYVSL
Sbjct: 64  ADSYVGRFWTITVASILYQIGMVSLTTSAVVPGLRPPPCKAGEICKEATGGQLGILYVSL 123

Query: 138 LLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAVTVLVYVQD 197
           LLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAVTVLVYVQD
Sbjct: 124 LLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAVTVLVYVQD 183

Query: 198 NIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLLQVGVAAYRKRKVCTVSD 257
           NIGWGWGFGIPTIAMLV             HLDPSGSPFTRLLQVGVAAY+KRKVC VSD
Sbjct: 184 NIGWGWGFGIPTIAMLVSIITFVVGYPIYRHLDPSGSPFTRLLQVGVAAYKKRKVCIVSD 243

Query: 258 SNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVGEELEKPNLWKLSTVHR 317
           SNLLYQN DIDDPISRDG L+HTKHMRFLDKAAIVTEEEKVK+GEEL KPNLWKLSTVHR
Sbjct: 244 SNLLYQNRDIDDPISRDGNLLHTKHMRFLDKAAIVTEEEKVKMGEELGKPNLWKLSTVHR 303

Query: 318 VEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTNSFQIPAGSMTVFTLLT 377
           VEELKSVIRMGPIWASGIILITAYSQQ TFSLLQASTMNRHLTNSFQIPAGSMTVFTLLT
Sbjct: 304 VEELKSVIRMGPIWASGIILITAYSQQYTFSLLQASTMNRHLTNSFQIPAGSMTVFTLLT 363

Query: 378 MLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATFIAGFAEQKRKHVAFTH 437
           ML TVALYDRVF+PIAR+FTGLDRGITFLTRMGIGLVISIFAT IAGF EQKRKHVAFTH
Sbjct: 364 MLITVALYDRVFIPIARRFTGLDRGITFLTRMGIGLVISIFATLIAGFVEQKRKHVAFTH 423

Query: 438 GLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS 497
           GL+DHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS
Sbjct: 424 GLIDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMALFWTSIS 483

Query: 498 AGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLITLLQVLNLVYYIVCAKF 557
           AGNYLST+LVSLV KYS GANGENWIRNDNI+KGKLENFYWL+T LQVLNL YYIVCAKF
Sbjct: 484 AGNYLSTLLVSLVHKYSSGANGENWIRNDNISKGKLENFYWLVTGLQVLNLAYYIVCAKF 543

Query: 558 YTFKPLEVQRKVVDSSKVDELQLVNPV 572
           YTFKPLEVQRKVVDSSKVDELQLVNPV
Sbjct: 544 YTFKPLEVQRKVVDSSKVDELQLVNPV 570

BLAST of PI0009077 vs. ExPASy TrEMBL
Match: A0A6J1K1R1 (protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita maxima OX=3661 GN=LOC111489833 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.3e-285
Identity = 495/583 (84.91%), Postives = 530/583 (90.91%), Query Frame = 0

Query: 2   EMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTIT 61
           E +    H+ERKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMP TK ANTIT
Sbjct: 3   EQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTIT 62

Query: 62  NFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEI 121
           NF+GTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPC GGEI
Sbjct: 63  NFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKGGEI 122

Query: 122 CKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAM 181
           C+EASG QLGILY SLLLTALGSGGIRPCVVSFGADQF+E DPK+G KTYKYFNWYYFAM
Sbjct: 123 CEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFAM 182

Query: 182 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLLQ 241
           GASMLVAVTVLVYVQDNIGWGWGFGIPTIAM +             HLDPSGSPFTRLLQ
Sbjct: 183 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLLQ 242

Query: 242 VGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVG 301
           V VAA+RKRK+  V +S LLYQNHDIDDPISRDGKL+HT HMRFLDKAAIVTE++KVKVG
Sbjct: 243 VSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVKVG 302

Query: 302 EELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTN 361
              EKPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSL Q +TM+R LT+
Sbjct: 303 ---EKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTD 362

Query: 362 SFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATF 421
           SF+IPAGSM+VFTLLTML T+ALYDR+F+PIAR+FTGLDRGITFL RMGIGLVISIFAT 
Sbjct: 363 SFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATL 422

Query: 422 IAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 481
           +AGF E+KRKHVAF HGL DHP+S IPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSP
Sbjct: 423 VAGFVERKRKHVAFIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 482

Query: 482 ESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLIT 541
           ESMRSTAMALFWTSISAGNYLST+LV+LV KY+ G NG NW+RNDNINKGKLENFYWL+T
Sbjct: 483 ESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVT 542

Query: 542 LLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 572
           LLQV NLVYY+VCAK YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 543 LLQVFNLVYYVVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of PI0009077 vs. ExPASy TrEMBL
Match: A0A6J1FKQ8 (protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita moschata OX=3662 GN=LOC111445106 PE=3 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.4e-283
Identity = 493/582 (84.71%), Postives = 528/582 (90.72%), Query Frame = 0

Query: 2   EMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTIT 61
           E +    H+ERKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMP TK ANTIT
Sbjct: 3   EHQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTIT 62

Query: 62  NFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEI 121
           NF+GTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGL+PPPC GGEI
Sbjct: 63  NFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGGEI 122

Query: 122 CKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAM 181
           C+EASG QLGILY SLLLTALGSGGIRPCVVSFGADQF+E DPK+G KTYKYFNWYYFAM
Sbjct: 123 CEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFAM 182

Query: 182 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLLQ 241
           GASMLVAVTVLVYVQDNIGWGWGFGIPTIAM +             HLDPSGSPFTRLLQ
Sbjct: 183 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLLQ 242

Query: 242 VGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVG 301
           V VAA+RKRK+  V +S LLYQNHDIDDPISRDGKL+HT HMRFLDKAAIVTE++KVKVG
Sbjct: 243 VSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVKVG 302

Query: 302 EELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTN 361
              EKPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSL Q +TM+R LT+
Sbjct: 303 ---EKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTD 362

Query: 362 SFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATF 421
           SF+IPAGSM+VFTLLTML T+ALYDR+F+PIAR+FTGLDRGITFL RMGIGLVISIFAT 
Sbjct: 363 SFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATL 422

Query: 422 IAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 481
           +AGF E+KRKHVA  HGL DHP+S IPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSP
Sbjct: 423 VAGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 482

Query: 482 ESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLIT 541
           ESMRSTAMALFWTSISAGNYLST+LV+LV KY+ G NG NW+RNDNINKGKLENFYWL+T
Sbjct: 483 ESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVT 542

Query: 542 LLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNP 571
           LLQV NLVYYIVCAK YTFKPLEVQRKVVDSSKVDE+QLVNP
Sbjct: 543 LLQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNP 581

BLAST of PI0009077 vs. ExPASy TrEMBL
Match: A0A6J1C163 (protein NRT1/ PTR FAMILY 3.1 OS=Momordica charantia OX=3673 GN=LOC111007508 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 8.9e-275
Identity = 486/583 (83.36%), Postives = 515/583 (88.34%), Query Frame = 0

Query: 2   EMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTIT 61
           E + K KH  RKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMP T+AANTIT
Sbjct: 3   EEQTKNKHAGRKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTQAANTIT 62

Query: 62  NFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEI 121
           NFSGTASLTPLIGAFIADSY GRFWTITVASILYQIGM  LTTSAVVP LRPPPCNGGE+
Sbjct: 63  NFSGTASLTPLIGAFIADSYAGRFWTITVASILYQIGMTILTTSAVVPTLRPPPCNGGEV 122

Query: 122 CKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAM 181
           C +A GGQLGILY+SLLLTALGSGGIRPCVVSFGADQFEE DPKRG KTYKYFNWYYFAM
Sbjct: 123 CSQADGGQLGILYISLLLTALGSGGIRPCVVSFGADQFEESDPKRGMKTYKYFNWYYFAM 182

Query: 182 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLLQ 241
           GASMLVAVTVLVYVQDNIGWGWGFGIPTIAM +             HLDPSGSPFTRLLQ
Sbjct: 183 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIVTFIVGYPIYRHLDPSGSPFTRLLQ 242

Query: 242 VGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVG 301
           VGVAAYRKRK+  VSD  +LYQNH+IDDPISRDGKL+HTKHM+FLDKAAIVTEE+++K G
Sbjct: 243 VGVAAYRKRKLDPVSDYKMLYQNHEIDDPISRDGKLLHTKHMKFLDKAAIVTEEDELKTG 302

Query: 302 EELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTN 361
               KPNLWKL+TVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSL QASTMNRH+T 
Sbjct: 303 ---RKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLQQASTMNRHVTE 362

Query: 362 SFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATF 421
           SFQIPAGSMTVFTLLTML TVALYDRVFVPIAR+FTGLDRGITFL RMGIGLVIS+FAT 
Sbjct: 363 SFQIPAGSMTVFTLLTMLVTVALYDRVFVPIARRFTGLDRGITFLRRMGIGLVISVFATL 422

Query: 422 IAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 481
           IAGF E+KRKH A             PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP
Sbjct: 423 IAGFVEKKRKHAAAGD----------PISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 482

Query: 482 ESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLIT 541
           ESMRSTAMALFWTSISAGNYLST+LV+LV K++ G  G NW+RNDN+N GKLENFYWLIT
Sbjct: 483 ESMRSTAMALFWTSISAGNYLSTLLVTLVHKFTAGPGGSNWLRNDNLNLGKLENFYWLIT 542

Query: 542 LLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 572
           LLQV NLVYY+ CAK YTFKPLE+Q KVVDSSKVDE+QLVN V
Sbjct: 543 LLQVFNLVYYVFCAKVYTFKPLELQGKVVDSSKVDEVQLVNHV 572

BLAST of PI0009077 vs. NCBI nr
Match: XP_004150669.1 (protein NRT1/ PTR FAMILY 3.1 [Cucumis sativus] >KAE8646611.1 hypothetical protein Csa_005320 [Cucumis sativus])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 545/584 (93.32%), Postives = 557/584 (95.38%), Query Frame = 0

Query: 1   MEMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTI 60
           MEMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMPSTKAANTI
Sbjct: 1   MEMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPSTKAANTI 60

Query: 61  TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGE 120
           TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGM+SLTTSAVVPGLRPPPC  GE
Sbjct: 61  TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMVSLTTSAVVPGLRPPPCKAGE 120

Query: 121 ICKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA 180
           ICKEA+GGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA
Sbjct: 121 ICKEATGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA 180

Query: 181 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLL 240
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV             HLDPSGSPFTRLL
Sbjct: 181 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLVSIITFVVGYPIYRHLDPSGSPFTRLL 240

Query: 241 QVGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKV 300
           QVGVAAY+KRKVC VSDSNLLYQN DIDDPISRDG L+HTKHMRFLDKAAIVTEEEKVK+
Sbjct: 241 QVGVAAYKKRKVCIVSDSNLLYQNRDIDDPISRDGNLLHTKHMRFLDKAAIVTEEEKVKM 300

Query: 301 GEELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLT 360
           GEEL KPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSLLQASTMNRHLT
Sbjct: 301 GEELGKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQASTMNRHLT 360

Query: 361 NSFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFAT 420
           NSFQIPAGSMTVFTLLTML TVALYDRVF+PIAR+FTGLDRGITFLTRMGIGLVISIFAT
Sbjct: 361 NSFQIPAGSMTVFTLLTMLITVALYDRVFIPIARRFTGLDRGITFLTRMGIGLVISIFAT 420

Query: 421 FIAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 480
            IAGF EQKRKHVAFTHGL+DHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 421 LIAGFVEQKRKHVAFTHGLIDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 480

Query: 481 PESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLI 540
           PESMRSTAMALFWTSISAGNYLST+LVSLV KYS GANGENWIRNDNI+KGKLENFYWL+
Sbjct: 481 PESMRSTAMALFWTSISAGNYLSTLLVSLVHKYSSGANGENWIRNDNISKGKLENFYWLV 540

Query: 541 TLLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 572
           T LQVLNL YYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV
Sbjct: 541 TGLQVLNLAYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 584

BLAST of PI0009077 vs. NCBI nr
Match: XP_008445656.1 (PREDICTED: protein NRT1/ PTR FAMILY 3.1 [Cucumis melo])

HSP 1 Score: 1069.7 bits (2765), Expect = 8.8e-309
Identity = 541/584 (92.64%), Postives = 555/584 (95.03%), Query Frame = 0

Query: 1   MEMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTI 60
           MEMEK EKHMERKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMPSTKAANTI
Sbjct: 1   MEMEKTEKHMERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPSTKAANTI 60

Query: 61  TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGE 120
           TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPC  GE
Sbjct: 61  TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKAGE 120

Query: 121 ICKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA 180
           ICKEA+ GQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA
Sbjct: 121 ICKEATEGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA 180

Query: 181 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLL 240
           MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV             HLDPSGSPF+RLL
Sbjct: 181 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLVSIITFVVGYPIYRHLDPSGSPFSRLL 240

Query: 241 QVGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKV 300
           QVGVAAYRKRKV +VSDSNLLYQN DID PISRDG L+HTKHMRFLDKAA+VTEEEKVK+
Sbjct: 241 QVGVAAYRKRKVGSVSDSNLLYQNRDIDGPISRDGVLLHTKHMRFLDKAAVVTEEEKVKM 300

Query: 301 GEELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLT 360
           GEEL KPNLWKLSTVHRVEELKSVIRMGPIWA+GIILITAYSQQNTFSLLQASTMNRHLT
Sbjct: 301 GEELGKPNLWKLSTVHRVEELKSVIRMGPIWAAGIILITAYSQQNTFSLLQASTMNRHLT 360

Query: 361 NSFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFAT 420
           NSFQIPAGSMTVFTLLTML TVALYDRVF+PIARKFTGLDRGITFLTRMGIGLVISIFAT
Sbjct: 361 NSFQIPAGSMTVFTLLTMLITVALYDRVFIPIARKFTGLDRGITFLTRMGIGLVISIFAT 420

Query: 421 FIAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 480
            IAGF EQKRKHVAF HGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS
Sbjct: 421 LIAGFVEQKRKHVAFNHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 480

Query: 481 PESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLI 540
           PESMRSTAMALFWTSISAG+YLST+LVSLV KYSGGANGENWIRNDNINKGKLENFYWLI
Sbjct: 481 PESMRSTAMALFWTSISAGSYLSTLLVSLVHKYSGGANGENWIRNDNINKGKLENFYWLI 540

Query: 541 TLLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 572
           T+LQVLNL YYIVCA+FYTFKPLEVQRKV+D SKVDELQLVNPV
Sbjct: 541 TVLQVLNLAYYIVCARFYTFKPLEVQRKVMDGSKVDELQLVNPV 584

BLAST of PI0009077 vs. NCBI nr
Match: XP_038884610.1 (protein NRT1/ PTR FAMILY 3.1 [Benincasa hispida])

HSP 1 Score: 1023.1 bits (2644), Expect = 9.4e-295
Identity = 521/589 (88.46%), Postives = 544/589 (92.36%), Query Frame = 0

Query: 3   MEKKEK------HMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKA 62
           ME+K K      H+ERKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMP TK 
Sbjct: 1   MEEKVKKQTNTNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKG 60

Query: 63  ANTITNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPC 122
           ANTITNF+GTASLTPLIGAFIADSYVGRFWTITVASILYQIGMI+LTTSAVVPGLRPPPC
Sbjct: 61  ANTITNFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMITLTTSAVVPGLRPPPC 120

Query: 123 NGGEICKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNW 182
            GGEIC+EASGGQLGILY SLLLTALGSGGIRPCVVSFGADQF+E DPK+GTKTYKYFNW
Sbjct: 121 KGGEICEEASGGQLGILYFSLLLTALGSGGIRPCVVSFGADQFDETDPKQGTKTYKYFNW 180

Query: 183 YYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPF 242
           YYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAM +             HLDPSGSPF
Sbjct: 181 YYFAMGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSILTFVVGYPIYRHLDPSGSPF 240

Query: 243 TRLLQVGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEE 302
           TRLLQV VAA+RKRK+ +   SNLLY NHDIDDPISRDGKL+HTKHMRFLDKAAIVTEE+
Sbjct: 241 TRLLQVAVAAFRKRKLHSPYHSNLLYHNHDIDDPISRDGKLLHTKHMRFLDKAAIVTEED 300

Query: 303 KVKVG-EELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTM 362
           KVK+G E+LEKPNLWKL+TVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSLLQ STM
Sbjct: 301 KVKMGREDLEKPNLWKLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLLQGSTM 360

Query: 363 NRHLTNSFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVI 422
           +RHLTNSFQIPAGSMTVFTLLTML TVALYDR+FVPIARKFTGLDRGITFL RMGIGLVI
Sbjct: 361 DRHLTNSFQIPAGSMTVFTLLTMLITVALYDRIFVPIARKFTGLDRGITFLKRMGIGLVI 420

Query: 423 SIFATFIAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEF 482
           SIFATFIAGF E+KRKHVAFTHGL DHP S IPISVFWLVPQYSLHGMAEAFMSIGHLEF
Sbjct: 421 SIFATFIAGFVERKRKHVAFTHGLEDHPGSTIPISVFWLVPQYSLHGMAEAFMSIGHLEF 480

Query: 483 FYDQSPESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLEN 542
           FYDQSPESMRSTAMALFWTSISAGNYLST+LV+LV KYS G NG NWIRNDNINKGKLEN
Sbjct: 481 FYDQSPESMRSTAMALFWTSISAGNYLSTLLVTLVHKYSAGPNGYNWIRNDNINKGKLEN 540

Query: 543 FYWLITLLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 572
           FYWL+TLLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV
Sbjct: 541 FYWLVTLLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 589

BLAST of PI0009077 vs. NCBI nr
Match: XP_022994014.1 (protein NRT1/ PTR FAMILY 3.1 [Cucurbita maxima])

HSP 1 Score: 990.3 bits (2559), Expect = 6.7e-285
Identity = 495/583 (84.91%), Postives = 530/583 (90.91%), Query Frame = 0

Query: 2   EMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTIT 61
           E +    H+ERKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMP TK ANTIT
Sbjct: 3   EQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTIT 62

Query: 62  NFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEI 121
           NF+GTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPC GGEI
Sbjct: 63  NFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCKGGEI 122

Query: 122 CKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAM 181
           C+EASG QLGILY SLLLTALGSGGIRPCVVSFGADQF+E DPK+G KTYKYFNWYYFAM
Sbjct: 123 CEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFAM 182

Query: 182 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLLQ 241
           GASMLVAVTVLVYVQDNIGWGWGFGIPTIAM +             HLDPSGSPFTRLLQ
Sbjct: 183 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLLQ 242

Query: 242 VGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVG 301
           V VAA+RKRK+  V +S LLYQNHDIDDPISRDGKL+HT HMRFLDKAAIVTE++KVKVG
Sbjct: 243 VSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAIVTEQDKVKVG 302

Query: 302 EELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTN 361
              EKPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSL Q +TM+R LT+
Sbjct: 303 ---EKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTD 362

Query: 362 SFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATF 421
           SF+IPAGSM+VFTLLTML T+ALYDR+F+PIAR+FTGLDRGITFL RMGIGLVISIFAT 
Sbjct: 363 SFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATL 422

Query: 422 IAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 481
           +AGF E+KRKHVAF HGL DHP+S IPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSP
Sbjct: 423 VAGFVERKRKHVAFIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 482

Query: 482 ESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLIT 541
           ESMRSTAMALFWTSISAGNYLST+LV+LV KY+ G NG NW+RNDNINKGKLENFYWL+T
Sbjct: 483 ESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVT 542

Query: 542 LLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 572
           LLQV NLVYY+VCAK YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 543 LLQVFNLVYYVVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of PI0009077 vs. NCBI nr
Match: XP_023550855.1 (protein NRT1/ PTR FAMILY 3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 985.7 bits (2547), Expect = 1.7e-283
Identity = 492/583 (84.39%), Postives = 529/583 (90.74%), Query Frame = 0

Query: 2   EMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTIT 61
           E +    H+ERKKGGLITMPFIFANEICEKIAVVGF+TNMISYLTKQLHMP TK ANTIT
Sbjct: 3   EQQSTRNHVERKKGGLITMPFIFANEICEKIAVVGFNTNMISYLTKQLHMPLTKGANTIT 62

Query: 62  NFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEI 121
           NF+GTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGL+PPPC GGEI
Sbjct: 63  NFNGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLKPPPCKGGEI 122

Query: 122 CKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAM 181
           C+EASG QLGILY SLLLTALGSGGIRPCVVSFGADQF+E DPK+G KTYKYFNWYYFAM
Sbjct: 123 CEEASGKQLGILYFSLLLTALGSGGIRPCVVSFGADQFDESDPKQGKKTYKYFNWYYFAM 182

Query: 182 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLLQ 241
           GASMLVAVTVLVYVQDNIGWGWGFGIPTIAM +             HLDPSGSPFTRLLQ
Sbjct: 183 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMFLSIITFVLGYSIYRHLDPSGSPFTRLLQ 242

Query: 242 VGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVG 301
           V VAA+RKRK+  V +S LLYQNHDIDDPISRDGKL+HT HMRFLDKAA+VTE++KVK+G
Sbjct: 243 VSVAAFRKRKLEMVPNSTLLYQNHDIDDPISRDGKLLHTNHMRFLDKAAVVTEQDKVKLG 302

Query: 302 EELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTN 361
              EKPNLW L+TVHRVEELKSVIRMGPIWASGIILITAYSQQ TFSL Q +TM+R LT+
Sbjct: 303 ---EKPNLWNLNTVHRVEELKSVIRMGPIWASGIILITAYSQQYTFSLQQGTTMDRRLTD 362

Query: 362 SFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATF 421
           SF+IPAGSM+VFTLLTML T+ALYDR+F+PIAR+FTGLDRGITFL RMGIGLVISIFAT 
Sbjct: 363 SFKIPAGSMSVFTLLTMLITIALYDRIFIPIARRFTGLDRGITFLKRMGIGLVISIFATL 422

Query: 422 IAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 481
           +AGF E+KRKHVA  HGL DHP+S IPIS+FWLVPQYSLHGMAEAFMSIGHLEFFYDQSP
Sbjct: 423 VAGFVERKRKHVALIHGLQDHPSSTIPISIFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 482

Query: 482 ESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLIT 541
           ESMRSTAMALFWTSISAGNYLST+LV+LV KY+ G NG NW+RNDNINKGKLENFYWL+T
Sbjct: 483 ESMRSTAMALFWTSISAGNYLSTLLVTLVHKYTAGPNGYNWLRNDNINKGKLENFYWLVT 542

Query: 542 LLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKVDELQLVNPV 572
           LLQV NLVYYIVCAK YTFKPLEVQRKVVDSSKVDE+QLVNPV
Sbjct: 543 LLQVFNLVYYIVCAKMYTFKPLEVQRKVVDSSKVDEVQLVNPV 582

BLAST of PI0009077 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 804.3 bits (2076), Expect = 6.4e-233
Identity = 405/582 (69.59%), Postives = 474/582 (81.44%), Query Frame = 0

Query: 4   EKKEKH--MERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTIT 63
           E+K+ H    R KGGLITMPFIFANEICEK+AVVGF  NMISYLT QLH+P TKAANT+T
Sbjct: 12  EEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLT 71

Query: 64  NFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEI 123
           NF+GT+SLTPL+GAFIADS+ GRFWTIT ASI+YQIGM  LT SA++P LRPPPC G E+
Sbjct: 72  NFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV 131

Query: 124 CKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAM 183
           C  A   QL ILYV+LLL ALGSGGIRPCVV+FGADQF+E DP + TKT+ YFNWYYF M
Sbjct: 132 CVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCM 191

Query: 184 GASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLV-------------HLDPSGSPFTRLLQ 243
           GA++L+AVTVLV++QDN+GWG G GIPT+AM +             HL P+GSPFTRL+Q
Sbjct: 192 GAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQ 251

Query: 244 VGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVG 303
           VGVAA+RKRK+  VSD +LLY N +ID PIS  GKL HTKHM FLDKAAIVTEE+ +K G
Sbjct: 252 VGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVTEEDNLKPG 311

Query: 304 EELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTN 363
              + PN W+LSTVHRVEELKSVIRMGPI ASGI+LITAY+QQ TFSL QA TMNRHLTN
Sbjct: 312 ---QIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTN 371

Query: 364 SFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATF 423
           SFQIPAGSM+VFT + MLTT+  YDRVFV +ARKFTGL+RGITFL RMGIG VISI AT 
Sbjct: 372 SFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATL 431

Query: 424 IAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSP 483
           +AGF E KRK VA  HGL+D P +I+PIS  WL+PQY LHG+AEAFMSIGHLEFFYDQ+P
Sbjct: 432 VAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAP 491

Query: 484 ESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLIT 543
           ESMRSTA ALFW +IS GNY+ST+LV+LV K+S   +G NW+ ++N+N+G+LE FYWLIT
Sbjct: 492 ESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLIT 551

Query: 544 LLQVLNLVYYIVCAKFYTFKPLEVQRKVVDSSKV-DELQLVN 570
           +LQ +NLVYY+ CAK YT+KP++V     DSS V +ELQL N
Sbjct: 552 VLQAVNLVYYLWCAKIYTYKPVQVHHSKEDSSPVKEELQLSN 590

BLAST of PI0009077 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 482.6 bits (1241), Expect = 4.2e-136
Identity = 260/561 (46.35%), Postives = 362/561 (64.53%), Query Frame = 0

Query: 1   MEMEKKEKHMERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTI 60
           +++ KK  + + K G      FI   E CE++A  G STN+I+YL KQ++M +  A+ ++
Sbjct: 14  LDIHKKPAN-KNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSV 73

Query: 61  TNFSGTASLTPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGE 120
           +N+SGT   TPLIGAFIAD+Y+GR+WTI    ++Y  GM  LT SA VPGL  P C+ GE
Sbjct: 74  SNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTCS-GE 133

Query: 121 ICKEASGGQLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFA 180
            C  A+ GQ  I +++L L ALG+GGI+PCV SFGADQF++ D K       +FNW+YF 
Sbjct: 134 TC-HATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFV 193

Query: 181 MGASMLVAVTVLVYVQDNIGWGWGFGIPTIAMLVHL-------------DPSGSPFTRLL 240
           +    ++A +VLV++Q N+GWGWG G+PT+AM + +              P GSP TR+L
Sbjct: 194 INVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRML 253

Query: 241 QVGVAAYRKRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKV 300
           QV VA+ RK KV    D +LLY+N D +  I    KL HTK + F DKAA+ TE +    
Sbjct: 254 QVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESD---- 313

Query: 301 GEELEKPNLWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLT 360
            +   K + WKL TV +VEELK++IR+ PIWA+GI+  + YSQ  T  +LQ +T+++H+ 
Sbjct: 314 NKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMG 373

Query: 361 NSFQIPAGSMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFAT 420
            +F+IP+ S+++F  L++L    +YD++ VP ARK+TG +RG T L R+GIGLVISIF+ 
Sbjct: 374 PNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSM 433

Query: 421 FIAGFAEQKRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQS 480
             AG  E  R +   TH L +  T  IP+++FW VPQY L G AE F  IG LEFFYDQ+
Sbjct: 434 VSAGILEVARLNYVQTHNLYNEET--IPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQA 493

Query: 481 PESMRSTAMALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLI 540
           P++MRS   AL  T+I+ GNYLST LV+LV K +       WI   N+N G L+ F+WL+
Sbjct: 494 PDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAK-NLNNGHLDYFFWLL 553

Query: 541 TLLQVLNLVYYIVCAKFYTFK 549
             L  LN + Y+  AK+YT+K
Sbjct: 554 AGLSFLNFLVYLWIAKWYTYK 563

BLAST of PI0009077 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 478.8 bits (1231), Expect = 6.1e-135
Identity = 248/551 (45.01%), Postives = 353/551 (64.07%), Query Frame = 0

Query: 11  ERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTITNFSGTASLT 70
           + K G      FI  NE CE++A  G  TN+++YL  +L+  +  AAN +TN+SGT  +T
Sbjct: 22  KEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYIT 81

Query: 71  PLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEICKEASGGQL 130
           PLIGAFIAD+Y+GR+WTI     +Y  GM  LT SA VPGL+P  CN  + C   +  Q 
Sbjct: 82  PLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN-ADTC-HPNSSQT 141

Query: 131 GILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAVT 190
            + +V+L + ALG+GGI+PCV SFGADQF+E D     K   +FNW+YF++    L+A T
Sbjct: 142 AVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAAT 201

Query: 191 VLVYVQDNIGWGWGFGIPTIAMLVHL-------------DPSGSPFTRLLQVGVAAYRKR 250
           VLV++Q N+GWGWGFG+PT+AM++ +              P GSP TR+ QV VAA+RK 
Sbjct: 202 VLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKI 261

Query: 251 KVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVGEELEKPNLW 310
            V    D +LL++  D +  I    KLVHT +++F DKAA+ ++ + +K GE     N W
Sbjct: 262 SVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGE----VNPW 321

Query: 311 KLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTNSFQIPAGSM 370
           +L +V +VEELKS+I + P+WA+GI+  T YSQ +T  +LQ +TM++H+  +F+IP+ S+
Sbjct: 322 RLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSASL 381

Query: 371 TVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATFIAGFAEQKR 430
           ++F  +++L    +YD+  +P+ARKFT  +RG T L RMGIGLV+SIFA   AG  E  R
Sbjct: 382 SLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVR 441

Query: 431 KHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAMA 490
                TH   D     I +S+FW +PQY L G AE F  IG LEFFYDQ+P++MRS   A
Sbjct: 442 LDYVKTHNAYDQKQ--IHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSA 501

Query: 491 LFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLITLLQVLNLVY 549
           L  T+++ GNYLST+LV++V K +       WI  DN+N+G L+ F++L+  L  LN + 
Sbjct: 502 LSLTTVALGNYLSTVLVTVVMKITKKNGKPGWI-PDNLNRGHLDYFFYLLATLSFLNFLV 561

BLAST of PI0009077 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 444.9 bits (1143), Expect = 9.8e-125
Identity = 242/552 (43.84%), Postives = 340/552 (61.59%), Query Frame = 0

Query: 10  MERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTITNFSGTASL 69
           ++ K G     PFI  NE CE++A  G + N+I+YLT +LH  +  AA  +T + GT  L
Sbjct: 38  LKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYL 97

Query: 70  TPLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNGGEICKEASGGQ 129
           TPLIGA +AD+Y GR+WTI   S +Y IGM +LT SA VP L+P  C  G+ C  A+  Q
Sbjct: 98  TPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECI-GDFCPSATPAQ 157

Query: 130 LGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVAV 189
             + +  L L ALG+GGI+PCV SFGADQF++ D +   +   +FNW+YF++    LV+ 
Sbjct: 158 YAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSS 217

Query: 190 TVLVYVQDNIGWGWGFGIPTIAMLVHL-------------DPSGSPFTRLLQVGVAAYRK 249
           ++LV++Q+N GWG GFGIPT+ M + +              P GSP TR+ QV VA++RK
Sbjct: 218 SLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRK 277

Query: 250 RKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVGEELEKPNL 309
             V    D+ LLY+  D +  I+   K+ HT   ++LDKAA+++EEE  K G   +  N 
Sbjct: 278 SSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEEES-KSG---DYSNS 337

Query: 310 WKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTNSFQIPAGS 369
           W+L TV +VEELK +IRM PIWASGII    Y+Q +T  + Q   MN  +  SFQ+P  +
Sbjct: 338 WRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPAA 397

Query: 370 MTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATFIAGFAEQK 429
           +  F   +++  V LYDR  VP+ARKFTG+D+G T + RMGIGL +S+     A   E  
Sbjct: 398 LGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEII 457

Query: 430 RKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTAM 489
           R H+A   GL++     +PISV W +PQY + G AE F  IG LEFFYDQSP++MRS   
Sbjct: 458 RLHMANDLGLVESGAP-VPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCS 517

Query: 490 ALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLITLLQVLNLV 549
           AL   + + GNYLS+++++LV  ++     E WI +DN+N G L+ F+WL+  L ++N+ 
Sbjct: 518 ALALLTNALGNYLSSLILTLVTYFTTRNGQEGWI-SDNLNSGHLDYFFWLLAGLSLVNMA 577

BLAST of PI0009077 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 2.5e-120
Identity = 235/556 (42.27%), Postives = 339/556 (60.97%), Query Frame = 0

Query: 11  ERKKGGLITMPFIFANEICEKIAVVGFSTNMISYLTKQLHMPSTKAANTITNFSGTASLT 70
           ++K G     PFI  NE CE++A  G + N+I+Y T +LH  +  AA+ +  + GT  +T
Sbjct: 50  KKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYIT 109

Query: 71  PLIGAFIADSYVGRFWTITVASILYQIGMISLTTSAVVPGLRPPPCNG--GEICKEASGG 130
           PLIGA IADSY GR+WTI   S +Y IGM  LT SA +P L+P  C G    +C  A+  
Sbjct: 110 PLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCSPATTV 169

Query: 131 QLGILYVSLLLTALGSGGIRPCVVSFGADQFEEGDPKRGTKTYKYFNWYYFAMGASMLVA 190
           Q  + +  L L ALG+GGI+PCV SFGADQF++ DP+   +   +FNW+YF++     ++
Sbjct: 170 QYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGSFIS 229

Query: 191 VTVLVYVQDNIGWGWGFGIPTIAMLVHL-------------DPSGSPFTRLLQVGVAAYR 250
            T+LV+VQ+N+GWG GF IPT+ M V +              P GSP TR+ QV VAAYR
Sbjct: 230 STLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVAAYR 289

Query: 251 KRKVCTVSDSNLLYQNHDIDDPISRDGKLVHTKHMRFLDKAAIVTEEEKVKVGEELEKPN 310
           K K+    D + LY+  + +  I+   K+ HT   +FLDKAA+++E E  K G      N
Sbjct: 290 KLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYES-KSG---AFSN 349

Query: 311 LWKLSTVHRVEELKSVIRMGPIWASGIILITAYSQQNTFSLLQASTMNRHLTNSFQIPAG 370
            WKL TV +VEE+K++IRM PIWASGI+    YSQ +T  + Q  +MNR +  SF+IP  
Sbjct: 350 PWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNR-IIRSFEIPPA 409

Query: 371 SMTVFTLLTMLTTVALYDRVFVPIARKFTGLDRGITFLTRMGIGLVISIFATFIAGFAEQ 430
           S  VF  L +L ++ +YDR  VP  R+FTG+ +G+T L RMGIGL +S+ +   A   E 
Sbjct: 410 SFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVET 469

Query: 431 KRKHVAFTHGLMDHPTSIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQSPESMRSTA 490
            R  +A            + +S+FW +PQY L G+AE F  IG +EFFYD+SP++MRS  
Sbjct: 470 VRLQLA---------QDFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSVC 529

Query: 491 MALFWTSISAGNYLSTMLVSLVEKYSGGANGENWIRNDNINKGKLENFYWLITLLQVLNL 550
            AL   + + G+YLS+++++LV  ++     + W+  D++NKG L+ F+WL+  L ++N+
Sbjct: 530 SALALLNTAVGSYLSSLILTLVAYFTALGGKDGWV-PDDLNKGHLDYFFWLLVSLGLVNI 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX209.0e-23269.59Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q9LFB86.0e-13546.35Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Q9M3908.6e-13445.01Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
P460321.4e-12343.84Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z203.5e-11942.27Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BD884.2e-30992.64protein NRT1/ PTR FAMILY 3.1 OS=Cucumis melo OX=3656 GN=LOC103488612 PE=3 SV=1[more]
A0A0A0K9535.7e-29891.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G037500 PE=3 SV=1[more]
A0A6J1K1R13.3e-28584.91protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita maxima OX=3661 GN=LOC111489833 PE=3 SV... [more]
A0A6J1FKQ81.4e-28384.71protein NRT1/ PTR FAMILY 3.1 OS=Cucurbita moschata OX=3662 GN=LOC111445106 PE=3 ... [more]
A0A6J1C1638.9e-27583.36protein NRT1/ PTR FAMILY 3.1 OS=Momordica charantia OX=3673 GN=LOC111007508 PE=3... [more]
Match NameE-valueIdentityDescription
XP_004150669.10.0e+0093.32protein NRT1/ PTR FAMILY 3.1 [Cucumis sativus] >KAE8646611.1 hypothetical protei... [more]
XP_008445656.18.8e-30992.64PREDICTED: protein NRT1/ PTR FAMILY 3.1 [Cucumis melo][more]
XP_038884610.19.4e-29588.46protein NRT1/ PTR FAMILY 3.1 [Benincasa hispida][more]
XP_022994014.16.7e-28584.91protein NRT1/ PTR FAMILY 3.1 [Cucurbita maxima][more]
XP_023550855.11.7e-28384.39protein NRT1/ PTR FAMILY 3.1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G68570.16.4e-23369.59Major facilitator superfamily protein [more]
AT5G01180.14.2e-13646.35peptide transporter 5 [more]
AT3G54140.16.1e-13545.01peptide transporter 1 [more]
AT2G02040.19.8e-12543.84peptide transporter 2 [more]
AT1G62200.12.5e-12042.27Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 85..502
e-value: 5.2E-88
score: 295.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 10..556
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..553
e-value: 4.6E-177
score: 591.4
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 17..543
NoneNo IPR availablePANTHERPTHR11654:SF544BNAA07G24560D PROTEINcoord: 10..556
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 74..98

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009077.1PI0009077.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010336 gibberellic acid homeostasis
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0097708 intracellular vesicle
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity