PI0008925 (gene) Melon (PI 482460) v1

Overview
NamePI0008925
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionaspartic proteinase CDR1-like
Locationchr07: 5683971 .. 5685263 (-)
RNA-Seq ExpressionPI0008925
SyntenyPI0008925
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTACCATTTCCATTTTCTTCCATCTAATCCTCTTCTTGATCTCCTTCTCTCAAACAACCATTATTAATGGTGATAATGGCTTCACCACCTCTCTCTTCCACCGCGATTCCCTTCTTTCGCCTCTTGAATTTTCATCTCTATCTCATTACGACCGCCTCACCAATGCCTTTCGTCGCTCGTTGTCCCGCTCCGTTGCTCTCCTCAATCGCGTTGCCACTGGTGGTGCTATTGTCCTCCAATCCCCAATTGCCCCAGGAAGTGGCGAGTATCTAATGTCCATCTCCATTGGAACCCCACCAGTTGACTACATAGGCATTGCTGACACAGGCAGTGATCTGACGTGGGCTCAATGCTTGCCATGTCTGAAATGTTTCAACCAATCACATCCCATTTTCAACCCTCTCAAATCCACATCCTTCAGTCACGTGCCTTGCACTTCCCAGATCTGTCAAGCTATTGATGATGCCCATTGTGGGGTCCAGGGGGTTTGCGATTACAGTTACACGTACGGAGATCAAACTTACACAAAGGGGGAGTTGGGATTTGAAAAGATCACCCTCAGGTCATCTTCTGTGCAATCAGTCATCGGATGTGGCCATGAGAGTGGTGGCGGATTTGGGTTTGCTTCAGGTGTCGTTGGACTTGGTGGGGGCCAACTCTCCTTAGTCTCACAAATGAGCCAAACCTCCGCGATCAGTCGTCGATTCTCTTATTGCTTACCAACACTACTCAGTCATGCAAATGGAAAAATAAACTTTGGCCAAAACGCTATCGTTTTTGGCCCTGGAGTCGTTTCAACGCCACTGATCTCCAAAAAGCCCGACACTTACTATTACATCACTTTGGAAGCCATTTCCATTGGCAACAAACGTCACATGGCCTCTGCCAAACAAGGCAATGTGATTATCGACAGTGGGACGACATTAACGGTTCTTCCAAAGGAGTTGTATGATGGTGTCGTTTCGTCGCTACTCAAGGTTGTTAAAGCAAAGCGAGTGATGGATCCCGGTAGCTTCTTTGAAGTATGCTTTGATGATGGCATCAATGCCTTTGCCTCCTCGGGTATTCCGATTATCACTGCACATTTTTCCGGTGGCGCTGACGTGAATTTGTTGCCGGTGAATACGTTTCAGAAGGTGGCAAGTAATGTGAATTGTTTGACATTAACAGCTGCATCACCAACAGACGAATTTGGGATAATAGGAAATTTGGCGCAGGCCAATTTCTTGATCGGATATGATTTGGAGGTCAAGAGATTGTCATTCAAGCCAAACGTTTGTGCTTAG

mRNA sequence

ATGGCGGCTACCATTTCCATTTTCTTCCATCTAATCCTCTTCTTGATCTCCTTCTCTCAAACAACCATTATTAATGGTGATAATGGCTTCACCACCTCTCTCTTCCACCGCGATTCCCTTCTTTCGCCTCTTGAATTTTCATCTCTATCTCATTACGACCGCCTCACCAATGCCTTTCGTCGCTCGTTGTCCCGCTCCGTTGCTCTCCTCAATCGCGTTGCCACTGGTGGTGCTATTGTCCTCCAATCCCCAATTGCCCCAGGAAGTGGCGAGTATCTAATGTCCATCTCCATTGGAACCCCACCAGTTGACTACATAGGCATTGCTGACACAGGCAGTGATCTGACGTGGGCTCAATGCTTGCCATGTCTGAAATGTTTCAACCAATCACATCCCATTTTCAACCCTCTCAAATCCACATCCTTCAGTCACGTGCCTTGCACTTCCCAGATCTGTCAAGCTATTGATGATGCCCATTGTGGGGTCCAGGGGGTTTGCGATTACAGTTACACGTACGGAGATCAAACTTACACAAAGGGGGAGTTGGGATTTGAAAAGATCACCCTCAGGTCATCTTCTGTGCAATCAGTCATCGGATGTGGCCATGAGAGTGGTGGCGGATTTGGGTTTGCTTCAGGTGTCGTTGGACTTGGTGGGGGCCAACTCTCCTTAGTCTCACAAATGAGCCAAACCTCCGCGATCAGTCGTCGATTCTCTTATTGCTTACCAACACTACTCAGTCATGCAAATGGAAAAATAAACTTTGGCCAAAACGCTATCGTTTTTGGCCCTGGAGTCGTTTCAACGCCACTGATCTCCAAAAAGCCCGACACTTACTATTACATCACTTTGGAAGCCATTTCCATTGGCAACAAACGTCACATGGCCTCTGCCAAACAAGGCAATGTGATTATCGACAGTGGGACGACATTAACGGTTCTTCCAAAGGAGTTGTATGATGGTGTCGTTTCGTCGCTACTCAAGGTTGTTAAAGCAAAGCGAGTGATGGATCCCGGTAGCTTCTTTGAAGTATGCTTTGATGATGGCATCAATGCCTTTGCCTCCTCGGGTATTCCGATTATCACTGCACATTTTTCCGGTGGCGCTGACGTGAATTTGTTGCCGGTGAATACGTTTCAGAAGGTGGCAAGTAATGTGAATTGTTTGACATTAACAGCTGCATCACCAACAGACGAATTTGGGATAATAGGAAATTTGGCGCAGGCCAATTTCTTGATCGGATATGATTTGGAGGTCAAGAGATTGTCATTCAAGCCAAACGTTTGTGCTTAG

Coding sequence (CDS)

ATGGCGGCTACCATTTCCATTTTCTTCCATCTAATCCTCTTCTTGATCTCCTTCTCTCAAACAACCATTATTAATGGTGATAATGGCTTCACCACCTCTCTCTTCCACCGCGATTCCCTTCTTTCGCCTCTTGAATTTTCATCTCTATCTCATTACGACCGCCTCACCAATGCCTTTCGTCGCTCGTTGTCCCGCTCCGTTGCTCTCCTCAATCGCGTTGCCACTGGTGGTGCTATTGTCCTCCAATCCCCAATTGCCCCAGGAAGTGGCGAGTATCTAATGTCCATCTCCATTGGAACCCCACCAGTTGACTACATAGGCATTGCTGACACAGGCAGTGATCTGACGTGGGCTCAATGCTTGCCATGTCTGAAATGTTTCAACCAATCACATCCCATTTTCAACCCTCTCAAATCCACATCCTTCAGTCACGTGCCTTGCACTTCCCAGATCTGTCAAGCTATTGATGATGCCCATTGTGGGGTCCAGGGGGTTTGCGATTACAGTTACACGTACGGAGATCAAACTTACACAAAGGGGGAGTTGGGATTTGAAAAGATCACCCTCAGGTCATCTTCTGTGCAATCAGTCATCGGATGTGGCCATGAGAGTGGTGGCGGATTTGGGTTTGCTTCAGGTGTCGTTGGACTTGGTGGGGGCCAACTCTCCTTAGTCTCACAAATGAGCCAAACCTCCGCGATCAGTCGTCGATTCTCTTATTGCTTACCAACACTACTCAGTCATGCAAATGGAAAAATAAACTTTGGCCAAAACGCTATCGTTTTTGGCCCTGGAGTCGTTTCAACGCCACTGATCTCCAAAAAGCCCGACACTTACTATTACATCACTTTGGAAGCCATTTCCATTGGCAACAAACGTCACATGGCCTCTGCCAAACAAGGCAATGTGATTATCGACAGTGGGACGACATTAACGGTTCTTCCAAAGGAGTTGTATGATGGTGTCGTTTCGTCGCTACTCAAGGTTGTTAAAGCAAAGCGAGTGATGGATCCCGGTAGCTTCTTTGAAGTATGCTTTGATGATGGCATCAATGCCTTTGCCTCCTCGGGTATTCCGATTATCACTGCACATTTTTCCGGTGGCGCTGACGTGAATTTGTTGCCGGTGAATACGTTTCAGAAGGTGGCAAGTAATGTGAATTGTTTGACATTAACAGCTGCATCACCAACAGACGAATTTGGGATAATAGGAAATTTGGCGCAGGCCAATTTCTTGATCGGATATGATTTGGAGGTCAAGAGATTGTCATTCAAGCCAAACGTTTGTGCTTAG

Protein sequence

MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGELGFEKITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSYCLPTLLSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPIITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVKRLSFKPNVCA
Homology
BLAST of PI0008925 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.0e-86
Identity = 193/451 (42.79%), Postives = 269/451 (59.65%), Query Frame = 0

Query: 11  LILFLISFSQTTIING-DNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSRSVAL 70
           L+ F + FS T   +G    F+  L HRDS LSP+    ++  DRL  AF RS+SRS   
Sbjct: 6   LLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRF 65

Query: 71  LNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLKCFNQ 130
            ++++      LQS +    GE+ MSI+IGTPP+    IADTGSDLTW QC PC +C+ +
Sbjct: 66  NHQLSQTD---LQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKE 125

Query: 131 SHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGV---QGVCDYSYTYGDQTYTKGELGFEK 190
           + PIF+  KS+++   PC S+ CQA+     G      +C Y Y+YGDQ+++KG++  E 
Sbjct: 126 NGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATET 185

Query: 191 ITLRSSSVQ------SVIGCGHESGGGFG-FASGVVGLGGGQLSLVSQMSQTSAISRRFS 250
           +++ S+S        +V GCG+ +GG F    SG++GLGGG LSL+SQ+   S+IS++FS
Sbjct: 186 VSIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLG--SSISKKFS 245

Query: 251 YCLPTLLSHANGK--INFGQNAIVFG----PGVVSTPLISKKPDTYYYITLEAISIGNKR 310
           YCL    +  NG   IN G N+I        GVVSTPL+ K+P TYYY+TLEAIS+G K+
Sbjct: 246 YCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKK 305

Query: 311 -------------HMASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLK-VVKAKRVMDPG 370
                         + S   GN+IIDSGTTLT+L    +D   S++ + V  AKRV DP 
Sbjct: 306 IPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQ 365

Query: 371 SFFEVCFDDGINAFASSGIPIITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDE 430
                CF  G    A  G+P IT HF+ GADV L P+N F K++ ++ CL++    PT E
Sbjct: 366 GLLSHCFKSG---SAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMV---PTTE 425

BLAST of PI0008925 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.6e-82
Identity = 188/446 (42.15%), Postives = 258/446 (57.85%), Query Frame = 0

Query: 6   SIFFHLILFLISFSQTTIINGDN----GFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRR 65
           S+F  ++L L   S   + N +     GFT  L HRDS  SP      +   RL NA  R
Sbjct: 3   SLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHR 62

Query: 66  SLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCL 125
           S++R      +  T      Q  +   SGEYLM++SIGTPP   + IADTGSDL W QC 
Sbjct: 63  SVNRVFHFTEKDNTPQP---QIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 122

Query: 126 PCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDD-AHCGV-QGVCDYSYTYGDQTYTK 185
           PC  C+ Q  P+F+P  S+++  V C+S  C A+++ A C      C YS +YGD +YTK
Sbjct: 123 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 182

Query: 186 GELGFEKITLRSSSVQS------VIGCGHESGGGFG-FASGVVGLGGGQLSLVSQMSQTS 245
           G +  + +TL SS  +       +IGCGH + G F    SG+VGLGGG +SL+ Q+    
Sbjct: 183 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLG--D 242

Query: 246 AISRRFSYCLPTLLSHAN--GKINFGQNAIVFGPGVVSTPLISK-KPDTYYYITLEAISI 305
           +I  +FSYCL  L S  +   KINFG NAIV G GVVSTPLI+K   +T+YY+TL++IS+
Sbjct: 243 SIDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISV 302

Query: 306 GNKRHMAS-----AKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEV 365
           G+K+   S     + +GN+IIDSGTTLT+LP E Y  +  ++   + A++  DP S   +
Sbjct: 303 GSKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSL 362

Query: 366 CFDDGINAFASSGIPIITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIG 425
           C+    +A     +P+IT HF  GADV L   N F +V+ ++ C      SP+  F I G
Sbjct: 363 CY----SATGDLKVPVITMHFD-GADVKLDSSNAFVQVSEDLVCFAF-RGSPS--FSIYG 422

Query: 426 NLAQANFLIGYDLEVKRLSFKPNVCA 431
           N+AQ NFL+GYD   K +SFKP  CA
Sbjct: 423 NVAQMNFLVGYDTVSKTVSFKPTDCA 435

BLAST of PI0008925 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 4.0e-59
Identity = 153/413 (37.05%), Postives = 212/413 (51.33%), Query Frame = 0

Query: 29  GFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSRSVALLNRVATGGAIVLQSPIAPG 88
           GF   L H DS  +  +F  L   +R      R L R  A+LN     G   +++ +  G
Sbjct: 40  GFQIMLEHVDSGKNLTKFQLL---ERAIERGSRRLQRLEAMLN-----GPSGVETSVYAG 99

Query: 89  SGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLKCFNQSHPIFNPLKSTSFSHVPCT 148
            GEYLM++SIGTP   +  I DTGSDL W QC PC +CFNQS PIFNP  S+SFS +PC+
Sbjct: 100 DGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCS 159

Query: 149 SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGELGFEKITLRSSSVQSV-IGCGHESGG- 208
           SQ+CQA+    C     C Y+Y YGD + T+G +G E +T  S S+ ++  GCG  + G 
Sbjct: 160 SQLCQALSSPTCS-NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGF 219

Query: 209 GFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSYCLPTLLSHANGKINFGQNAIVFGPGV 268
           G G  +G+VG+G G LSL SQ+  T     +FSYC+  + S     +  G  A     G 
Sbjct: 220 GQGNGAGLVGMGRGPLSLPSQLDVT-----KFSYCMTPIGSSTPSNLLLGSLANSVTAGS 279

Query: 269 VSTPLI-SKKPDTYYYITLEAISIGNKR---------HMASAKQGNVIIDSGTTLTVLPK 328
            +T LI S +  T+YYITL  +S+G+ R           ++   G +IIDSGTTLT    
Sbjct: 280 PNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVN 339

Query: 329 ELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPIITAHFSGGADVNLLPV 388
             Y  V    +  +    V    S F++CF    +  ++  IP    HF GG D+ L   
Sbjct: 340 NAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDP-SNLQIPTFVMHFDGG-DLELPSE 399

Query: 389 NTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVKRLSFKPNVC 430
           N F   ++ + CL +   S +    I GN+ Q N L+ YD     +SF    C
Sbjct: 400 NYFISPSNGLICLAM--GSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of PI0008925 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.4e-56
Identity = 141/395 (35.70%), Postives = 207/395 (52.41%), Query Frame = 0

Query: 48  SLSHYDRLTNAFRRS--LSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDY 107
           +L+ Y+ +  A +R     RS+  + + ++G    +++P+  G GEYLM+++IGTP   +
Sbjct: 54  NLTKYELIKRAIKRGERRMRSINAMLQSSSG----IETPVYAGDGEYLMNVAIGTPDSSF 113

Query: 108 IGIADTGSDLTWAQCLPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGV 167
             I DTGSDL W QC PC +CF+Q  PIFNP  S+SFS +PC SQ CQ +    C     
Sbjct: 114 SAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCN-NNE 173

Query: 168 CDYSYTYGDQTYTKGELGFEKITLRSSSVQSV-IGCGHESGG-GFGFASGVVGLGGGQLS 227
           C Y+Y YGD + T+G +  E  T  +SSV ++  GCG ++ G G G  +G++G+G G LS
Sbjct: 174 CQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLS 233

Query: 228 LVSQMSQTSAISRRFSYCLPTLLSHANGKINFGQNAIVFGPGVVSTPLI-SKKPDTYYYI 287
           L SQ+        +FSYC+ +  S +   +  G  A     G  ST LI S    TYYYI
Sbjct: 234 LPSQLG-----VGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYI 293

Query: 288 TLEAISIGNKR--------HMASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKR 347
           TL+ I++G            +     G +IIDSGTTLT LP++ Y+ V  +    +    
Sbjct: 294 TLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPT 353

Query: 348 VMDPGSFFEVCFDDGINAFASSGIPIITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAA 407
           V +  S    CF    +  ++  +P I+  F GG  +NL   N     A  V CL + ++
Sbjct: 354 VDESSSGLSTCFQQPSDG-STVQVPEISMQFDGGV-LNLGEQNILISPAEGVICLAMGSS 413

Query: 408 SPTDEFGIIGNLAQANFLIGYDLEVKRLSFKPNVC 430
           S      I GN+ Q    + YDL+   +SF P  C
Sbjct: 414 SQLG-ISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of PI0008925 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 4.6e-55
Identity = 143/430 (33.26%), Postives = 219/430 (50.93%), Query Frame = 0

Query: 30  FTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSRSVALLNRVATGGAIV--------- 89
           +T  L HRD   S    +  +H+ RL    RR   R  A+L R++  G ++         
Sbjct: 59  YTLRLLHRDRFPS---VTYRNHHHRLHARMRRDTDRVSAILRRIS--GKVIPSSDSRYEV 118

Query: 90  ------LQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLKCFNQSHPIF 149
                 + S +  GSGEY + I +G+PP D   + D+GSD+ W QC PC  C+ QS P+F
Sbjct: 119 NDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVF 178

Query: 150 NPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGELGFEKITLRSSSV 209
           +P KS S++ V C S +C  I+++ C   G C Y   YGD +YTKG L  E +T   + V
Sbjct: 179 DPAKSGSYTGVSCGSSVCDRIENSGCH-SGGCRYEVMYGDGSYTKGTLALETLTFAKTVV 238

Query: 210 QSV-IGCGHESGGGFGFASGVVGLGGGQLSLVSQMS-QTSAISRRFSYCLPTLLSHANGK 269
           ++V +GCGH + G F  A+G++G+GGG +S V Q+S QT      F YCL +  + + G 
Sbjct: 239 RNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGG---AFGYCLVSRGTDSTGS 298

Query: 270 INFGQNAIVFGPGVVSTPLI-SKKPDTYYYITLEAISIGNKR--------HMASAKQGNV 329
           + FG+ A+  G   V  PL+ + +  ++YY+ L+ + +G  R         +     G V
Sbjct: 299 LVFGREALPVGASWV--PLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV 358

Query: 330 IIDSGTTLTVLPKELY----DGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIP 389
           ++D+GT +T LP   Y    DG  S    + +A  V    S F+ C+D  ++ F S  +P
Sbjct: 359 VMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGV----SIFDTCYD--LSGFVSVRVP 418

Query: 390 IITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEV 430
            ++ +F+ G  + L   N    V  +       AASPT    IIGN+ Q    + +D   
Sbjct: 419 TVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTG-LSIIGNIQQEGIQVSFDGAN 470

BLAST of PI0008925 vs. ExPASy TrEMBL
Match: A0A5A7TSV3 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G002730 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.7e-222
Identity = 389/429 (90.68%), Postives = 406/429 (94.64%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           M ATISIFF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MVATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
           RSLSRS ALLNR AT GA+ LQSPIAPGSGEYLMS+SIGTPPVDYIG+ADTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQC 120

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180
           LPC+KCF QS PIFNPLKSTSFSHVPC SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG
Sbjct: 121 LPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 ELGFEKITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSY 240
           +LG EKIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSY
Sbjct: 181 DLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQ 300
           CLPTLLSHANGKINFGQNA+V GPGVVSTPLISK P TYYYITLEAISIGN+RHMASAKQ
Sbjct: 241 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPI 360
           GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRV DPGSF+++CFDDGIN  ASSGIPI
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVK 420
           IT HFSGGA+VNLLPVNTFQKVA+NVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLE K
Sbjct: 361 ITTHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPNVC 430
           RLSFKP VC
Sbjct: 421 RLSFKPTVC 429

BLAST of PI0008925 vs. ExPASy TrEMBL
Match: A0A5D3CX41 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003090 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.0e-222
Identity = 388/424 (91.51%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 6   SIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSR 65
           SIF  LILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRL+NAFRRSLSR
Sbjct: 4   SIFCRLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFRRSLSR 63

Query: 66  SVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLK 125
           S ALLNR AT GA+ LQSPIAPGSGEYLMS+SIGTPPVDYIG+ADTGSDLTWAQCLPC+K
Sbjct: 64  SAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQCLPCVK 123

Query: 126 CFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGELGFE 185
           CF QS PIFNPLKSTSFSHVPC SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG+LG E
Sbjct: 124 CFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGDLGLE 183

Query: 186 KITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSYCLPTL 245
           KIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSYCLPTL
Sbjct: 184 KITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSYCLPTL 243

Query: 246 LSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQGNVII 305
           LSHANGKINFGQNA+V GPGVVSTPLISK P TYYYITLEAISIGN+RHMASAKQGNVII
Sbjct: 244 LSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQGNVII 303

Query: 306 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPIITAHF 365
           DSGTTLTVLPKELYDGVVSSLLKVVKAKRV DPGSF+++CFDDGIN  ASSGIPIITAHF
Sbjct: 304 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPIITAHF 363

Query: 366 SGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVKRLSFK 425
           SGGA+VNLLPVNTFQKVA+NVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLE KRLSFK
Sbjct: 364 SGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAKRLSFK 423

Query: 426 PNVC 430
           P VC
Sbjct: 424 PTVC 427

BLAST of PI0008925 vs. ExPASy TrEMBL
Match: A0A1S3BUB0 (probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493257 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.0e-222
Identity = 388/424 (91.51%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 6   SIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSR 65
           SIF  LILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRL+NAFRRSLSR
Sbjct: 4   SIFCRLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFRRSLSR 63

Query: 66  SVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLK 125
           S ALLNR AT GA+ LQSPIAPGSGEYLMS+SIGTPPVDYIG+ADTGSDLTWAQCLPC+K
Sbjct: 64  SAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQCLPCVK 123

Query: 126 CFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGELGFE 185
           CF QS PIFNPLKSTSFSHVPC SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG+LG E
Sbjct: 124 CFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGDLGLE 183

Query: 186 KITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSYCLPTL 245
           KIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSYCLPTL
Sbjct: 184 KITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSYCLPTL 243

Query: 246 LSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQGNVII 305
           LSHANGKINFGQNA+V GPGVVSTPLISK P TYYYITLEAISIGN+RHMASAKQGNVII
Sbjct: 244 LSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQGNVII 303

Query: 306 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPIITAHF 365
           DSGTTLTVLPKELYDGVVSSLLKVVKAKRV DPGSF+++CFDDGIN  ASSGIPIITAHF
Sbjct: 304 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPIITAHF 363

Query: 366 SGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVKRLSFK 425
           SGGA+VNLLPVNTFQKVA+NVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLE KRLSFK
Sbjct: 364 SGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAKRLSFK 423

Query: 426 PNVC 430
           P VC
Sbjct: 424 PTVC 427

BLAST of PI0008925 vs. ExPASy TrEMBL
Match: A0A5D3CYR2 (Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003080 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.8e-219
Identity = 386/429 (89.98%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           MAATISIFF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MAATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
           RSLSRS ALLNR AT GA+ LQSPIAPGSGEYLM +SIGTPPVDYIG+ DTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRTATSGAVGLQSPIAPGSGEYLMYVSIGTPPVDYIGMIDTGSDLTWAQC 120

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180
           LPC KCF Q  PIFNPLKSTSFSHVPC SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG
Sbjct: 121 LPCRKCFLQLRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 ELGFEKITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSY 240
           +LGFEKIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQ 300
           CLP LL HANGKINF QNA+V GPGVVSTPLISK P TYYYITLEAISIGN+RHMASAKQ
Sbjct: 241 CLPPLLGHANGKINFAQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPI 360
           GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRV DPGSF+++CFDDGIN  ASSGIPI
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVK 420
           ITAHFSGGA+VNLLPVNTFQKVA+NVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLE K
Sbjct: 361 ITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPNVC 430
           RLSFKP VC
Sbjct: 421 RLSFKPTVC 429

BLAST of PI0008925 vs. ExPASy TrEMBL
Match: A0A1S3BTZ4 (probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493256 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.8e-219
Identity = 386/429 (89.98%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           MAATISIFF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MAATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
           RSLSRS ALLNR AT GA+ LQSPIAPGSGEYLM +SIGTPPVDYIG+ DTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRTATSGAVGLQSPIAPGSGEYLMYVSIGTPPVDYIGMIDTGSDLTWAQC 120

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180
           LPC KCF Q  PIFNPLKSTSFSHVPC SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG
Sbjct: 121 LPCRKCFLQLRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 ELGFEKITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSY 240
           +LGFEKIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQ 300
           CLP LL HANGKINF QNA+V GPGVVSTPLISK P TYYYITLEAISIGN+RHMASAKQ
Sbjct: 241 CLPPLLGHANGKINFAQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPI 360
           GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRV DPGSF+++CFDDGIN  ASSGIPI
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVK 420
           ITAHFSGGA+VNLLPVNTFQKVA+NVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLE K
Sbjct: 361 ITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPNVC 430
           RLSFKP VC
Sbjct: 421 RLSFKPTVC 429

BLAST of PI0008925 vs. NCBI nr
Match: KAA0044967.1 (putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 781.6 bits (2017), Expect = 3.6e-222
Identity = 389/429 (90.68%), Postives = 406/429 (94.64%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           M ATISIFF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MVATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
           RSLSRS ALLNR AT GA+ LQSPIAPGSGEYLMS+SIGTPPVDYIG+ADTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQC 120

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180
           LPC+KCF QS PIFNPLKSTSFSHVPC SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG
Sbjct: 121 LPCVKCFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 ELGFEKITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSY 240
           +LG EKIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSY
Sbjct: 181 DLGLEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQ 300
           CLPTLLSHANGKINFGQNA+V GPGVVSTPLISK P TYYYITLEAISIGN+RHMASAKQ
Sbjct: 241 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPI 360
           GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRV DPGSF+++CFDDGIN  ASSGIPI
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVK 420
           IT HFSGGA+VNLLPVNTFQKVA+NVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLE K
Sbjct: 361 ITTHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPNVC 430
           RLSFKP VC
Sbjct: 421 RLSFKPTVC 429

BLAST of PI0008925 vs. NCBI nr
Match: XP_008452153.1 (PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16501.1 putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 780.0 bits (2013), Expect = 1.0e-221
Identity = 388/424 (91.51%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 6   SIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSR 65
           SIF  LILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRL+NAFRRSLSR
Sbjct: 4   SIFCRLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLSNAFRRSLSR 63

Query: 66  SVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLK 125
           S ALLNR AT GA+ LQSPIAPGSGEYLMS+SIGTPPVDYIG+ADTGSDLTWAQCLPC+K
Sbjct: 64  SAALLNRAATSGAVGLQSPIAPGSGEYLMSVSIGTPPVDYIGLADTGSDLTWAQCLPCVK 123

Query: 126 CFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGELGFE 185
           CF QS PIFNPLKSTSFSHVPC SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG+LG E
Sbjct: 124 CFKQSRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKGDLGLE 183

Query: 186 KITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSYCLPTL 245
           KIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSYCLPTL
Sbjct: 184 KITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSYCLPTL 243

Query: 246 LSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQGNVII 305
           LSHANGKINFGQNA+V GPGVVSTPLISK P TYYYITLEAISIGN+RHMASAKQGNVII
Sbjct: 244 LSHANGKINFGQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQGNVII 303

Query: 306 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPIITAHF 365
           DSGTTLTVLPKELYDGVVSSLLKVVKAKRV DPGSF+++CFDDGIN  ASSGIPIITAHF
Sbjct: 304 DSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPIITAHF 363

Query: 366 SGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVKRLSFK 425
           SGGA+VNLLPVNTFQKVA+NVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLE KRLSFK
Sbjct: 364 SGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAKRLSFK 423

Query: 426 PNVC 430
           P VC
Sbjct: 424 PTVC 427

BLAST of PI0008925 vs. NCBI nr
Match: XP_008452152.1 (PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16500.1 putative aspartic protease [Cucumis melo var. makuwa])

HSP 1 Score: 771.5 bits (1991), Expect = 3.7e-219
Identity = 386/429 (89.98%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           MAATISIFF L L LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFS+LSHYDRL+NAFR
Sbjct: 1   MAATISIFFLLFLLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSTLSHYDRLSNAFR 60

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
           RSLSRS ALLNR AT GA+ LQSPIAPGSGEYLM +SIGTPPVDYIG+ DTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRTATSGAVGLQSPIAPGSGEYLMYVSIGTPPVDYIGMIDTGSDLTWAQC 120

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180
           LPC KCF Q  PIFNPLKSTSFSHVPC SQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG
Sbjct: 121 LPCRKCFLQLRPIFNPLKSTSFSHVPCNSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180

Query: 181 ELGFEKITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSY 240
           +LGFEKIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQ 300
           CLP LL HANGKINF QNA+V GPGVVSTPLISK P TYYYITLEAISIGN+RHMASAKQ
Sbjct: 241 CLPPLLGHANGKINFAQNAVVSGPGVVSTPLISKDPVTYYYITLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPI 360
           GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRV DPGSF+++CFDDGIN  ASSGIPI
Sbjct: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVKDPGSFWDLCFDDGINVAASSGIPI 360

Query: 361 ITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVK 420
           ITAHFSGGA+VNLLPVNTFQKVA+NVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLE K
Sbjct: 361 ITAHFSGGANVNLLPVNTFQKVANNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPNVC 430
           RLSFKP VC
Sbjct: 421 RLSFKPTVC 429

BLAST of PI0008925 vs. NCBI nr
Match: XP_031739975.1 (probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649216.1 hypothetical protein Csa_014406 [Cucumis sativus])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 377/429 (87.88%), Postives = 400/429 (93.24%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           M ATISIFFHLIL LISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR
Sbjct: 1   MVATISIFFHLILLLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
           RSLSRS  LLNR AT GA+ LQ+P+ PGSGEYLMS+SIGTPPVDYIG+ADTGSDL WAQC
Sbjct: 61  RSLSRSATLLNRAATNGALDLQAPLTPGSGEYLMSVSIGTPPVDYIGMADTGSDLMWAQC 120

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180
           LPCLKC+ QS PIF+PLKSTSFSHVPC SQ C+AIDD+HCG QGVCDYSYTYGDQTYTKG
Sbjct: 121 LPCLKCYKQSRPIFDPLKSTSFSHVPCNSQNCKAIDDSHCGAQGVCDYSYTYGDQTYTKG 180

Query: 181 ELGFEKITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSY 240
           +LGFEKIT+ SSSV+SVIGCGHESGGGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHESGGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQ 300
           CLPTLLSHANGKINFGQNA+V GPGVVSTPLISK P TYYY+TLEAISIGN+RHMASAKQ
Sbjct: 241 CLPTLLSHANGKINFGQNAVVSGPGVVSTPLISKNPVTYYYVTLEAISIGNERHMASAKQ 300

Query: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPI 360
           GNVIIDSGTTL+ LPKELYDGVVSSLLKVVKAKRV DPG+F+++CFDDGIN   SSGIPI
Sbjct: 301 GNVIIDSGTTLSFLPKELYDGVVSSLLKVVKAKRVKDPGNFWDLCFDDGINVATSSGIPI 360

Query: 361 ITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVK 420
           ITA FSGGA+VNLLPVNTFQKVA+NVNCLTLT ASPTDEFGIIGNLA ANFLIGYDLE K
Sbjct: 361 ITAQFSGGANVNLLPVNTFQKVANNVNCLTLTPASPTDEFGIIGNLALANFLIGYDLEAK 420

Query: 421 RLSFKPNVC 430
           RLSFKP VC
Sbjct: 421 RLSFKPTVC 429

BLAST of PI0008925 vs. NCBI nr
Match: XP_004149005.3 (probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649217.1 hypothetical protein Csa_015005 [Cucumis sativus])

HSP 1 Score: 751.9 bits (1940), Expect = 3.0e-213
Identity = 372/430 (86.51%), Postives = 395/430 (91.86%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           MAATIS+FFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRL NAFR
Sbjct: 1   MAATISLFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLANAFR 60

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
           RSLSRS ALLNR AT GA+ LQS I PGSGEYLMS+SIGTPPVDY+GIADTGSDLTWAQC
Sbjct: 61  RSLSRSAALLNRAATSGAVGLQSSIGPGSGEYLMSVSIGTPPVDYLGIADTGSDLTWAQC 120

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180
           LPCLKC+ Q  PIFNPLKSTSFSHVPC +Q C A+DD HCGVQGVCDYSYTYGD+TY+KG
Sbjct: 121 LPCLKCYQQLRPIFNPLKSTSFSHVPCNTQTCHAVDDGHCGVQGVCDYSYTYGDRTYSKG 180

Query: 181 ELGFEKITLRSSSVQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAISRRFSY 240
           +LGFEKIT+ SSSV+SVIGCGH S GGFGFASGV+GLGGGQLSLVSQMSQTS ISRRFSY
Sbjct: 181 DLGFEKITIGSSSVKSVIGCGHASSGGFGFASGVIGLGGGQLSLVSQMSQTSGISRRFSY 240

Query: 241 CLPTLLSHANGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHMASAKQ 300
           CLPTLLSHANGKINFG+NA+V GPGVVSTPLISK   TYYYITLEAISIGN+RHMA AKQ
Sbjct: 241 CLPTLLSHANGKINFGENAVVSGPGVVSTPLISKNTVTYYYITLEAISIGNERHMAFAKQ 300

Query: 301 GNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGINAFASSGIPI 360
           GNVIIDSGTTLT+LPKELYDGVVSSLLKVVKAKRV DP    ++CFDDGINA AS GIP+
Sbjct: 301 GNVIIDSGTTLTILPKELYDGVVSSLLKVVKAKRVKDPHGSLDLCFDDGINAAASLGIPV 360

Query: 361 ITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQANFLIGYDLEVK 420
           ITAHFSGGA+VNLLP+NTF+KVA NVNCLTL AASPT EFGIIGNLAQANFLIGYDLE K
Sbjct: 361 ITAHFSGGANVNLLPINTFRKVADNVNCLTLKAASPTTEFGIIGNLAQANFLIGYDLEAK 420

Query: 421 RLSFKPNVCA 431
           RLSFKP VCA
Sbjct: 421 RLSFKPTVCA 430

BLAST of PI0008925 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 329.7 bits (844), Expect = 3.5e-90
Identity = 192/440 (43.64%), Postives = 263/440 (59.77%), Query Frame = 0

Query: 6   SIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSR 65
           S+ F  +L L+  S       D GFT  L HRDS  SP   S+ +   R+ NA RRS   
Sbjct: 3   SLIFATLLSLLLLSNVNAYPKD-GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSARS 62

Query: 66  SVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLK 125
           ++   N  A+  +   QS I    GEYLM+ISIGTPPV  + IADTGSDL W QC PC  
Sbjct: 63  TLQFSNDDASPNS--PQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCED 122

Query: 126 CFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGV-QGVCDYSYTYGDQTYTKGELGF 185
           C+ Q+ P+F+P +S+++  V C+S  C+A++DA C   +  C Y+ TYGD +YTKG++  
Sbjct: 123 CYQQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAV 182

Query: 186 EKITLRSSSVQSV------IGCGHESGGGFGFA-SGVVGLGGGQLSLVSQMSQTSAISRR 245
           + +T+ SS  + V      IGCGHE+ G F  A SG++GLGGG  SLVSQ+ +  +I+ +
Sbjct: 183 DTVTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRK--SINGK 242

Query: 246 FSYCLPTLLSHA--NGKINFGQNAIVFGPGVVSTPLISKKPDTYYYITLEAISIGNKRHM 305
           FSYCL    S      KINFG N IV G GVVST ++ K P TYY++ LEAIS+G+K+  
Sbjct: 243 FSYCLVPFTSETGLTSKINFGTNGIVSGDGVVSTSMVKKDPATYYFLNLEAISVGSKKIQ 302

Query: 306 ASAK-----QGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFDDGI 365
            ++      +GN++IDSGTTLT+LP   Y  + S +   +KA+RV DP     +C+ D  
Sbjct: 303 FTSTIFGTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCYRDS- 362

Query: 366 NAFASSGIPIITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIGNLAQAN 425
              +S  +P IT HF GG DV L  +NTF  V+ +V+C    A    ++  I GNLAQ N
Sbjct: 363 ---SSFKVPDITVHFKGG-DVKLGNLNTFVAVSEDVSCFAFAA---NEQLTIFGNLAQMN 422

Query: 426 FLIGYDLEVKRLSFKPNVCA 431
           FL+GYD     +SFK   C+
Sbjct: 423 FLVGYDTVSGTVSFKKTDCS 429

BLAST of PI0008925 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 322.0 bits (824), Expect = 7.2e-88
Identity = 193/451 (42.79%), Postives = 269/451 (59.65%), Query Frame = 0

Query: 11  LILFLISFSQTTIING-DNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRRSLSRSVAL 70
           L+ F + FS T   +G    F+  L HRDS LSP+    ++  DRL  AF RS+SRS   
Sbjct: 6   LLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRF 65

Query: 71  LNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCLPCLKCFNQ 130
            ++++      LQS +    GE+ MSI+IGTPP+    IADTGSDLTW QC PC +C+ +
Sbjct: 66  NHQLSQTD---LQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKE 125

Query: 131 SHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGV---QGVCDYSYTYGDQTYTKGELGFEK 190
           + PIF+  KS+++   PC S+ CQA+     G      +C Y Y+YGDQ+++KG++  E 
Sbjct: 126 NGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATET 185

Query: 191 ITLRSSSVQ------SVIGCGHESGGGFG-FASGVVGLGGGQLSLVSQMSQTSAISRRFS 250
           +++ S+S        +V GCG+ +GG F    SG++GLGGG LSL+SQ+   S+IS++FS
Sbjct: 186 VSIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLG--SSISKKFS 245

Query: 251 YCLPTLLSHANGK--INFGQNAIVFG----PGVVSTPLISKKPDTYYYITLEAISIGNKR 310
           YCL    +  NG   IN G N+I        GVVSTPL+ K+P TYYY+TLEAIS+G K+
Sbjct: 246 YCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKK 305

Query: 311 -------------HMASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLK-VVKAKRVMDPG 370
                         + S   GN+IIDSGTTLT+L    +D   S++ + V  AKRV DP 
Sbjct: 306 IPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQ 365

Query: 371 SFFEVCFDDGINAFASSGIPIITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDE 430
                CF  G    A  G+P IT HF+ GADV L P+N F K++ ++ CL++    PT E
Sbjct: 366 GLLSHCFKSG---SAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMV---PTTE 425

BLAST of PI0008925 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.8e-83
Identity = 188/446 (42.15%), Postives = 258/446 (57.85%), Query Frame = 0

Query: 6   SIFFHLILFLISFSQTTIINGDN----GFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFRR 65
           S+F  ++L L   S   + N +     GFT  L HRDS  SP      +   RL NA  R
Sbjct: 3   SLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHR 62

Query: 66  SLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQCL 125
           S++R      +  T      Q  +   SGEYLM++SIGTPP   + IADTGSDL W QC 
Sbjct: 63  SVNRVFHFTEKDNTPQP---QIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 122

Query: 126 PCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDD-AHCGV-QGVCDYSYTYGDQTYTK 185
           PC  C+ Q  P+F+P  S+++  V C+S  C A+++ A C      C YS +YGD +YTK
Sbjct: 123 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 182

Query: 186 GELGFEKITLRSSSVQS------VIGCGHESGGGFG-FASGVVGLGGGQLSLVSQMSQTS 245
           G +  + +TL SS  +       +IGCGH + G F    SG+VGLGGG +SL+ Q+    
Sbjct: 183 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLG--D 242

Query: 246 AISRRFSYCLPTLLSHAN--GKINFGQNAIVFGPGVVSTPLISK-KPDTYYYITLEAISI 305
           +I  +FSYCL  L S  +   KINFG NAIV G GVVSTPLI+K   +T+YY+TL++IS+
Sbjct: 243 SIDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISV 302

Query: 306 GNKRHMAS-----AKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEV 365
           G+K+   S     + +GN+IIDSGTTLT+LP E Y  +  ++   + A++  DP S   +
Sbjct: 303 GSKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSL 362

Query: 366 CFDDGINAFASSGIPIITAHFSGGADVNLLPVNTFQKVASNVNCLTLTAASPTDEFGIIG 425
           C+    +A     +P+IT HF  GADV L   N F +V+ ++ C      SP+  F I G
Sbjct: 363 CY----SATGDLKVPVITMHFD-GADVKLDSSNAFVQVSEDLVCFAF-RGSPS--FSIYG 422

Query: 426 NLAQANFLIGYDLEVKRLSFKPNVCA 431
           N+AQ NFL+GYD   K +SFKP  CA
Sbjct: 423 NVAQMNFLVGYDTVSKTVSFKPTDCA 435

BLAST of PI0008925 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 305.4 bits (781), Expect = 7.0e-83
Identity = 189/458 (41.27%), Postives = 259/458 (56.55%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           MA    ++  L+     F+  +  N +N  T  L HRDS  SPL     +  DRL  AF 
Sbjct: 1   MATKTFLYCSLLAISFFFASNSSANREN-LTVELIHRDSPHSPLYNPHHTVSDRLNAAFL 60

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
           RS+SRS     +        LQS +    GEY MSISIGTPP     IADTGSDLTW QC
Sbjct: 61  RSISRSRRFTTKTD------LQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQC 120

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGV---QGVCDYSYTYGDQTY 180
            PC +C+ Q+ P+F+  KS+++    C S+ CQA+ +   G    + +C Y Y+YGD ++
Sbjct: 121 KPCQQCYKQNSPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSF 180

Query: 181 TKGELGFEKITLRSSSVQS------VIGCGHESGGGF-GFASGVVGLGGGQLSLVSQMSQ 240
           TKG++  E I++ SSS  S      V GCG+ +GG F    SG++GLGGG LSLVSQ+  
Sbjct: 181 TKGDVATETISIDSSSGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLG- 240

Query: 241 TSAISRRFSYCLPTLLSHANGK--INFGQNAIVFGP----GVVSTPLISKKPDTYYYITL 300
            S+I ++FSYCL    +  NG   IN G N+I   P      ++TPLI K P+TYY++TL
Sbjct: 241 -SSIGKKFSYCLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTL 300

Query: 301 EAISIGNKR-----------HMASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLK-VVKA 360
           EA+++G  +             +S + GN+IIDSGTTLT+L    YD   +++ + V  A
Sbjct: 301 EAVTVGKTKLPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGA 360

Query: 361 KRVMDPGSFFEVCFDDGINAFASSGIPIITAHFSGGADVNLLPVNTFQKVASNVNCLTLT 420
           KRV DP      CF  G       G+P IT HF+  ADV L P+N F K+  +  CL++ 
Sbjct: 361 KRVSDPQGLLTHCFKSGDKEI---GLPAITMHFT-NADVKLSPINAFVKLNEDTVCLSMI 420

Query: 421 AASPTDEFGIIGNLAQANFLIGYDLEVKRLSFKPNVCA 431
              PT E  I GN+ Q +FL+GYDLE K +SF+   C+
Sbjct: 421 ---PTTEVAIYGNMVQMDFLVGYDLETKTVSFQRMDCS 442

BLAST of PI0008925 vs. TAIR 10
Match: AT2G28010.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 225.3 bits (573), Expect = 9.2e-59
Identity = 166/445 (37.30%), Postives = 220/445 (49.44%), Query Frame = 0

Query: 1   MAATISIFFHLILFLISFSQTTIINGDNGFTTSLFHRDSLLSPLEFSSLSHYDRLTNAFR 60
           +A TI + F  +   + F  TT  +  +GFT  L HR S  S           R++N   
Sbjct: 3   LATTIIVLF--LQISLCFLFTTTASPPHGFTMDLIHRRSNAS----------SRVSN--- 62

Query: 61  RSLSRSVALLNRVATGGAIVLQSPIAPGSGEYLMSISIGTPPVDYIGIADTGSDLTWAQC 120
            + S S    N V                  YLM + +GTPP +   I DTGS++TW QC
Sbjct: 63  -TQSGSSPYANTVFDNSV-------------YLMKLQVGTPPFEIQAIIDTGSEITWTQC 122

Query: 121 LPCLKCFNQSHPIFNPLKSTSFSHVPCTSQICQAIDDAHCGVQGVCDYSYTYGDQTYTKG 180
           LPC+ C+ Q+ PIF+P KS++F    C         D H      C Y   Y D TYT G
Sbjct: 123 LPCVHCYEQNAPIFDPSKSSTFKEKRC---------DGHS-----CPYEVDYFDHTYTMG 182

Query: 181 ELGFEKITLRSSS------VQSVIGCGHESGGGFGFASGVVGLGGGQLSLVSQMSQTSAI 240
            L  E ITL S+S       +++IGCGH +       SG+VGL  G  SL++QM      
Sbjct: 183 TLATETITLHSTSGEPFVMPETIIGCGHNNSWFKPSFSGMVGLNWGPSSLITQMG--GEY 242

Query: 241 SRRFSYCLPTLLSHANGKINFGQNAIVFGPGVVSTPLI--SKKPDTYYYITLEAISIGNK 300
               SYC          KINFG NAIV G GVVST +   + KP  +YY+ L+A+S+GN 
Sbjct: 243 PGLMSYCFS---GQGTSKINFGANAIVAGDGVVSTTMFMTTAKPG-FYYLNLDAVSVGNT 302

Query: 301 R-----HMASAKQGNVIIDSGTTLTVLPKELYDGVVSSLLKVVKAKRVMDPGSFFEVCFD 360
           R         A +GN++IDSGTTLT  P    + V  ++  VV A R  DP     +C++
Sbjct: 303 RIETMGTTFHALEGNIVIDSGTTLTYFPVSYCNLVRQAVEHVVTAVRAADPTGNDMLCYN 362

Query: 361 -DGINAFASSGIPIITAHFSGGADVNLLPVNTFQKVAS-NVNCLTLTAASPTDEFGIIGN 420
            D I+ F     P+IT HFSGG D+ L   N + +  +  V CL +   SPT E  I GN
Sbjct: 363 SDTIDIF-----PVITMHFSGGVDLVLDKYNMYMESNNGGVFCLAIICNSPTQE-AIFGN 392

Query: 421 LAQANFLIGYDLEVKRLSFKPNVCA 431
            AQ NFL+GYD     +SF P  C+
Sbjct: 423 RAQNNFLVGYDSSSLLVSFSPTNCS 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3EBM51.0e-8642.79Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Q6XBF82.6e-8242.15Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q766C34.0e-5937.05Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C22.4e-5635.70Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LHE34.6e-5533.26Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Match NameE-valueIdentityDescription
A0A5A7TSV31.7e-22290.68Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A5D3CX415.0e-22291.51Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BUB05.0e-22291.51probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493257 PE=... [more]
A0A5D3CYR21.8e-21989.98Putative aspartic protease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BTZ41.8e-21989.98probable aspartic protease At2g35615 OS=Cucumis melo OX=3656 GN=LOC103493256 PE=... [more]
Match NameE-valueIdentityDescription
KAA0044967.13.6e-22290.68putative aspartic protease [Cucumis melo var. makuwa][more]
XP_008452153.11.0e-22191.51PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16501.1 putat... [more]
XP_008452152.13.7e-21989.98PREDICTED: probable aspartic protease At2g35615 [Cucumis melo] >TYK16500.1 putat... [more]
XP_031739975.11.3e-21687.88probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649216.1 hypothetica... [more]
XP_004149005.33.0e-21386.51probable aspartic protease At2g35615 [Cucumis sativus] >KAE8649217.1 hypothetica... [more]
Match NameE-valueIdentityDescription
AT1G64830.13.5e-9043.64Eukaryotic aspartyl protease family protein [more]
AT2G35615.17.2e-8842.79Eukaryotic aspartyl protease family protein [more]
AT5G33340.11.8e-8342.15Eukaryotic aspartyl protease family protein [more]
AT1G31450.17.0e-8341.27Eukaryotic aspartyl protease family protein [more]
AT2G28010.19.2e-5937.30Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 280..425
e-value: 1.7E-24
score: 86.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 74..257
e-value: 1.6E-47
score: 164.0
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 260..430
e-value: 2.1E-41
score: 143.7
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 85..429
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 92..257
e-value: 3.3E-47
score: 161.0
NoneNo IPR availablePANTHERPTHR47967:SF66ASPARTIC PROTEINASE CDR1-RELATEDcoord: 4..429
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 4..429
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 303..314
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 92..425
score: 39.197784
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 91..429
e-value: 1.14081E-68
score: 217.131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0008925.1PI0008925.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity