PI0008472 (gene) Melon (PI 482460) v1

Overview
NamePI0008472
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionERAD-associated E3 ubiquitin-protein ligase HRD1B-like
Locationchr05: 2735781 .. 2742297 (+)
RNA-Seq ExpressionPI0008472
SyntenyPI0008472
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTCTCTGACATATTTATTTAAATTAAAAAACAATAAAGGGAACGCGAAATCCCTTTCGAGATTGTTCATCGTTTCCAGATTTTCGGTTTTGTTCTCAGATCGCGCTCTACTTTCGTCGGCTTCTCAGGTACTCTCCATCTTCTTGAATCTCTTTCTATAAGTTATTGTTGAACTTCGTTAGGTTTTCGAGATAGAGTGAGAGACGAAATGGAGCCGAAATTGCCTAATGTCTTGTGTTTTTTGTTGCGGTAAATTGGAAATTTATGTTTGGTGTGAATATTTTCTTTTGCTCGTAATTTCTGCTTCAGAATTTAGGGCTTTGTAATTGATTACGATTAATTTATGTTAGGCTTTGTATTTGGATTAGAACAAGCTATCGGTTCCCTATAAGCTTGTGGTTATAGGTTTGGAGCTTGGCACATTAGGTTTTTTTCCCTTCTGATTTTCCTTTATTCTTCGTTTTAGCAATTGGAGTCCTTTGCTCTTTTGGAATTTTTATATATTAATCGTTGCTCGATAAGCTTGTGGTTTAGAAGTCGAGTGGTAAACATCAATGGTGAAAAGATGTATTTTTCTTAATCTTTATTTATTTATTTCTTTTGGAATTCAAACAAATGTTAGTCTCGTTAGTTCTTCTATCAACATTCTCTTCTCCAAGAAAAACTCTTGAAGCACTTGAAGTAACCTCTTTATAATTTTTTGTTCTTTTTTGCTGAAAGGACTTCTGATTCCTTATCCCCTTAACCATTTTGTTTTGGAAAATTTAGCCTATAGATACTACTTTCACCTCCAAATTTCTTCCTTTGTTTTCTACTTTTTACCAATGGTTTATAAAACCAAGTCAAAACTTGAAAACTATAAAAAAGTAGCTTTTAGAAATTCGTTTTTGTTTTTGAAATTTGGCTAAGAATGAAACCATTGTACTCAAGGAAGATGCAAATCATTGTAAGAGATTGAGTGGAAATAGGCTTAATTTTCAAAAACTAAAAACGAAATGTTACCAAACGGGGCCTTAATTTTCCAAATACACACATGTTCTTTTGCAATCAATGTTTGTCAATGGTGATTCTGATTGCCCAACTTATCCTTAGAAAACTCCTCTGTCATTTCTTGCAAATGTCCTTTTATCTTATCATAAGGGCGTGGTAACATGATAATGAAACAATTTTGACTTTCTCTTTATGCATTTCTTCTCAGTTTAAACGTCTTACTGAAAGATAACTAATTCACAATATTTTAAAGATAGGTTCTGATGATATTTTAGCATCATTTAATTGTTTCATTTGGCTGACATTTCAACTTCTCATATGGCAGTGCAGTAGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTTATTGCAACCATAATGGTTATCTATCATGCCTTCAACAGTAGAGGGCAGTTCTACCCGGCATTGGTCTATTTATCGACTTCCAAAATAAATCTAGTACTTCTTCTCAATATGGGTTTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAAAAAATATTTCTGGGTTCTCTTAGAGAAGCAGAAGTTGAGAGGCTCAACGAGCAATCATGGAAAGAGTTGATGGAAATCCTCTTTGCCATCACTATTTTTCGGCAAGATTTTTCTGTTGCATTCCTGGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTCTGCATTGGTTGGCTCAGAAGAGAGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATTGTTTCTTTCTTAGTCTTCCTCTTTGTTCTCGATAGTCTGTTTTTTTATAATTCGATAGATTCTCTGATACAGACAAGGCCGGGCTCGGTTTCCCTCTTCTTTTCATTTGAGTAAGTTTTCAATTTCCATTGGTTCATATCTTTTTCTTTCCTTTTTTTTGGTCGGTGTTGTGGTTATAGGAAATAGACATATCATTATTATGTTGAAATTACGAAAGAGGAAAGTCCTATACAATGGGTTTCAAAATGCTTTTCCAATTGACCATGAGATTTGGTTCATATCTTGACTCTGTATTTAGACAAGTATTAGTTATGATGTTTCATAGTGAAATTTTTTGAACATTTGGTACCACATTAATTTGAAGGAGGTGTGGTTAGAATGTCAGAGATACCAAGGATATGTTAGATGAACTCACAAACTATTGTTTAAATTTGATACTTAAGTTCTTATATAGAGAATTTATGGCTTTAGGATTACTGTCTTGTAGTCTTTGCTCGGAAGAGTTAGGTTTTTAAATATCCTATTATGTTTCTTGTGCTCCTGAGAGGGTTTTATTTCAACATAAAATAAATTCAATGATTTGTTTCAGGTATATGATTCTTGCGACAACAGCTGTGTCAACATTTGTGAAATATATTTTCTATGTCAGTGATGTGCTCATGGAGGGACAATGGGAAAGGAAACCCGTCTATACATTTTACTTGGAACTTATTCGAGACTTACTTCATTTGTCGATGTACTTGTGTTTCTTTCTTGTGATTTTCATGTAAGTAATCAAACTACACCCGTGAGCTATCTCATTCTTGCATACTGGAAGTTCAGAATTAATCTCTCTCTGCATAAATGTTCTCATACTGCCTATATCTAAATGGGATTCTGATTCTTCTCCAGAAATACTAGGCGAACCAAAAAAAACCCAGATGTGGTTACTAGGATTTTTGTTGTCCTTTCATGTATCAATGGCTATCATATTTTGTTTTCATGTTAATTGTGGGAGATGGCTCATAAGTCATAGTAGGAGTGTCTTAAGAATGATAGGGTATTTTTTGTTTGTTTGGATATCCTTTACATCCTTATTATTTTCAATCAGTTGTTTTGAATTTTAGTCTCTTTAAGATTGAAAGAGGCAGAAATTTCTGTTTACATGCACACATTGCACAAGAAAAGAATGATTTTGCTTGTAGTCTGACCAATTATTTTTTGTATATTGCTAGACTGTAAACATCGTAGAAAGTTTAGAATTCATCTCATTTATTTGTTTGTCTTTTGAATGTGTTGTTTTCATCATATATATATGGTTATCTTTTGAACTTTGAATCGTCTGCTCTCCTTTCCCCAAGTATATTCTTACACCTACTGTTTAATAATAAGGAGATAAGGAAGCTAACTAAAGCTCAAGGACAAGTGTAATGTACAACTTTCTTGTCTTTATATGCATGATGTTTAGCACAATGGGATAGGAATCAATCGCCATCCCAAAGGTTGATGGTTTGATCCCCTACCCCACAGTTGTTGAACTTAAAATAATGTTTGGAGTTACATCCACACTAACTCCATTCCCATTTCACATCTTTATTCTACTGACTTTGACTTTGCTCTTGTAGGACTTATGGTGTGCCTTTGCACTTGATCCGAGAGCTTTATGAGATCTTTAGGAATTTTAGGATTCGCATTGCAGATTACATCCGTTATAGAAAGATTACTTCAAACATGAATGATCGTTTTCCAGATGCAACGCCTGAAGAACTCAATTCGTGCGTCCAAGCTTGGTCAACTTGTTGTGTCTTTGATGCTTCCAGTACTTTTATGTTAGCTACTAAAGTTCTTTTTATGGAATTGCAGAAGTGATGCAACATGCATTATTTGTCGTGAAGAGATGACAGTTGCAAAGAAACTTGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCTTGGTTGGAGCGACAACATACTTGTCCCACTTGTAGAGCCCTTGTTGTACCACCCGAAGGTTCAAGTACAACAAGCGGTCATCATGGAGTGCAGTCTGATGCTAACCAACAAGGTAATTGCACTCATAACAATTCAATTCAATTTACTGCCTGGCTAAAGGGTCCTACGTCTTGAACAATTTTTAATCTGGTAAGTATTATAAATTTCCTCCATGTGTTTTGTCTGGTTGTGGACCTTTTTGGAATCTTTAGTATGGAGAAAGTGTGTAAAAACTCTAGAATTTTGAAAAAGTTGGACTGAATAATTATTTGTAATTGGCTCAAAATGCCCCTTAAAAGGGCTGTTGATAGAGGTCTTGTTTCACTATTTGTTTTTTTATTTTTATTTTTTTGTATATGGAGTGTCCTTGGCGTTCTACTGGTGTGTCTTAGCATGTTGAAATGCAAATAATTAATAATAAAATAATGGACACACCTGTTAGAGTATTCGAGGTATGCGTAAAATGGCCTAGACACTCACGGATATAAAAATATAAAATAATGGACATGCCAGGCACGTGTCCTCACTGACCCTTTTTCCGAGCTTAGAAGTGTCAGTGCTTTATAAGAACCAAATTTGGTTTAATGTTTCAAAACTATTAAATAATCTTGATTATGAAGTAACCTTTCAAAAATAAGCTATCAATATTTGCAAGTATTCTCTGTATATAATATACCCCACCATCAGTGTGAAAGTTGAAATTTGAGCCTCTCTCAATTCATTGCATGGGTGTTGATTCTCTCTGCTCCCTTCTCTATTCTTATTTCTTTTGTTTTCATGATTGTTTTTCTTAAGAGACTGTCGTTAAAGTTGTAGTTTGTTTTATATATTTTTAAGCAATTCAATCTCTCCTAAAAGAAGTAGTTTGATTCTAGAACTGTCTATGAACTTAAGCATATTGAGAATGGATGATCATTATTGAACTGGACCACTGGTCCTCTGTAAAAATTGGGTGCTTCATTTTATTCTTGTTTTGTTTTATATTTTCTTATTGTGTTAGAAAAGGTGTAGGAGTAATTTCCGAAGTTCTCTATTGAGATACTGAAAAGGCTTTCTTTACCTAAAAAACTGTTTGAAGTCTTTTATATGATCTCATTTCTTTGGATTAACAACATTAAATGTTTGGTTTTTTTTGTGAATGCTTGTAGGAGTTCATTTAGTGTATCTCTGCTTTCTCTAATAGATGTTTTTGTTGCAAATATAGAACTGTTGGTTTCATTTAATTGTTTTTTCTTGATGGAGGTATACCATGCTGAAACTTGGATACAATGACTGAATTGTTTATTATGTTAATAACAGGAACAGGAACCACAAGTTCATCTGCACAAGGTACAAATGGCACAGGTGTTACTAATGATAATTTAAGTCATCATCAGGCTAGAGTCCGAGCTGCTGCTGCCGCTGCTTCAATATATGAGAAATCGTATGTTTATCCTTGTGCAAGCACATTAGTATGGTATGTCTTTTCATTACAAACTACGTGATCTCTGTTTTATTCTCCTTTATGTTGATCTAGAAAGTTTACAAAGGCCATTTCTCCTTTTGCAAAGAGAAAGAAGAGTATAATATTTTATTATTAATATTATTATTTGTTTATTTATTTACAGGTCTCTTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGTCTGACTCTAACAATACAGAAACAAATGGAGAGCAAACTCCCAATGGACATCCACAATACTTTTCATTTCCTGGCGGCCCTTCAAACCTGTCCTTCACTCAATTTCCCCAAAATATCTTTGTTCCTTTTCAGCCACGAGAGGGCACTGGAAACAATATAGAGGAATTGGGCAGTAGTCTAAATGTATCAGGCTCACAGCTTGAAGCTCAAGAAAAATTTCTTCAACAACAGATTGAGGTATGTGATCAACGTTACCTAGTGAGCTGTTTTGTTTGGTCAGTGCATGTTTTATTTAGGCTCTTGTTGCTGGGCCCTTGTATAATCTTCAGCTCTGCTTTTGAAGGAAAAAGGAAAAAAAAAAAACGTGAACAGTAGTTTACTAGTAGGCACACATGGCCTTGGCCATGGGCAAATAGTACTCATGAATCAATATCATTTCTCATACTTTTTTTTTTTTAAATGATCATTTCTCATACTTAGGTGATGTTTATGATGAATTCAAATTATCAGAATAACATGTCTTGATTCTCTCGTTGTCCTGGATTGACCTAACTGCTGTTTGGTTTTCCCATTTGTGCTGTTTTTATTTAATACCTTCTGTTAAATTCAAAATTTTGTATTTGCTAACAGTTCCTTCATAATCAACTGCAACTTCTCCGTAAACCAAAAGTTGAGGAAAGCTCAAGAGGAGCGAGCGAGGCAGATAAGAAAGGAAAAAGTTTAGCACCCTCACCATCGCCGTCGGTGTCATTGTCTTCATCAGATTCTCATCCGCCTGGAGAAACCGAAAGGGCGATAAGGTAAGATAGAATAAATGTGCATAATTATAGTGTAATAATACGAGACTGCAGATTGACACAATGACATAGATGTGTGTCTATTGTATACGGAAGATGTACTTGGTAACTTTTTAGAAGCAGTCTTCTTCCCCTTTGCCATATTGGTGGTTGGTTCGCTTGTAAATGTTGAATTTGGTCTCTTTTAAAGCAATCGATGCGACAGTTAAAAATTGTAGCAAAAGAAGAGTTTTTTTTCAAAGATGTTTGGTTGTTTCTATTTTCTCAAGTCTAAAAGATTTTTTTCTTGAACAGCAATTAAATTGACATGCGCATGAATGAGAAGACATTGATTGTTTGGAAG

mRNA sequence

ATTTTTCTCTGACATATTTATTTAAATTAAAAAACAATAAAGGGAACGCGAAATCCCTTTCGAGATTGTTCATCGTTTCCAGATTTTCGGTTTTGTTCTCAGATCGCGCTCTACTTTCGTCGGCTTCTCAGTGCAGTAGAGAATGATGAAGCTGCAAACATATGCGGGGATTAGTTTTATTGCAACCATAATGGTTATCTATCATGCCTTCAACAGTAGAGGGCAGTTCTACCCGGCATTGGTCTATTTATCGACTTCCAAAATAAATCTAGTACTTCTTCTCAATATGGGTTTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAAAAAATATTTCTGGGTTCTCTTAGAGAAGCAGAAGTTGAGAGGCTCAACGAGCAATCATGGAAAGAGTTGATGGAAATCCTCTTTGCCATCACTATTTTTCGGCAAGATTTTTCTGTTGCATTCCTGGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTCTGCATTGGTTGGCTCAGAAGAGAGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATTGTTTCTTTCTTAGTCTTCCTCTTTGTTCTCGATAGTCTGTTTTTTTATAATTCGATAGATTCTCTGATACAGACAAGGCCGGGCTCGGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCGACAACAGCTGTGTCAACATTTGTGAAATATATTTTCTATGTCAGTGATGTGCTCATGGAGGGACAATGGGAAAGGAAACCCGTCTATACATTTTACTTGGAACTTATTCGAGACTTACTTCATTTGTCGATGTACTTGTGTTTCTTTCTTGTGATTTTCATGACTTATGGTGTGCCTTTGCACTTGATCCGAGAGCTTTATGAGATCTTTAGGAATTTTAGGATTCGCATTGCAGATTACATCCGTTATAGAAAGATTACTTCAAACATGAATGATCGTTTTCCAGATGCAACGCCTGAAGAACTCAATTCAAGTGATGCAACATGCATTATTTGTCGTGAAGAGATGACAGTTGCAAAGAAACTTGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCTTGGTTGGAGCGACAACATACTTGTCCCACTTGTAGAGCCCTTGTTGTACCACCCGAAGGTTCAAGTACAACAAGCGGTCATCATGGAGTGCAGTCTGATGCTAACCAACAAGGAACAGGAACCACAAGTTCATCTGCACAAGGTACAAATGGCACAGGTGTTACTAATGATAATTTAAGTCATCATCAGGCTAGAGTCCGAGCTGCTGCTGCCGCTGCTTCAATATATGAGAAATCGTATGTTTATCCTTGTGCAAGCACATTAGTATGGTCTCTTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGTCTGACTCTAACAATACAGAAACAAATGGAGAGCAAACTCCCAATGGACATCCACAATACTTTTCATTTCCTGGCGGCCCTTCAAACCTGTCCTTCACTCAATTTCCCCAAAATATCTTTGTTCCTTTTCAGCCACGAGAGGGCACTGGAAACAATATAGAGGAATTGGGCAGTAGTCTAAATGTATCAGGCTCACAGCTTGAAGCTCAAGAAAAATTTCTTCAACAACAGATTGAGTTCCTTCATAATCAACTGCAACTTCTCCGTAAACCAAAAGTTGAGGAAAGCTCAAGAGGAGCGAGCGAGGCAGATAAGAAAGGAAAAAGTTTAGCACCCTCACCATCGCCGTCGGTGTCATTGTCTTCATCAGATTCTCATCCGCCTGGAGAAACCGAAAGGGCGATAAGGTAAGATAGAATAAATGTGCATAATTATAGTGTAATAATACGAGACTGCAGATTGACACAATGACATAGATGTGTGTCTATTGTATACGGAAGATGTACTTGGTAACTTTTTAGAAGCAGTCTTCTTCCCCTTTGCCATATTGGTGGTTGGTTCGCTTGTAAATGTTGAATTTGGTCTCTTTTAAAGCAATCGATGCGACAGTTAAAAATTGTAGCAAAAGAAGAGTTTTTTTTCAAAGATGTTTGGTTGTTTCTATTTTCTCAAGTCTAAAAGATTTTTTTCTTGAACAGCAATTAAATTGACATGCGCATGAATGAGAAGACATTGATTGTTTGGAAG

Coding sequence (CDS)

ATGATGAAGCTGCAAACATATGCGGGGATTAGTTTTATTGCAACCATAATGGTTATCTATCATGCCTTCAACAGTAGAGGGCAGTTCTACCCGGCATTGGTCTATTTATCGACTTCCAAAATAAATCTAGTACTTCTTCTCAATATGGGTTTAGTCATCATGTGCATTTTGTGGCAGCTAACAAAAAAAATATTTCTGGGTTCTCTTAGAGAAGCAGAAGTTGAGAGGCTCAACGAGCAATCATGGAAAGAGTTGATGGAAATCCTCTTTGCCATCACTATTTTTCGGCAAGATTTTTCTGTTGCATTCCTGGGTATGGTTACGGCTCTGTTGTTGATCAAAGCTCTGCATTGGTTGGCTCAGAAGAGAGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATTGTTTCTTTCTTAGTCTTCCTCTTTGTTCTCGATAGTCTGTTTTTTTATAATTCGATAGATTCTCTGATACAGACAAGGCCGGGCTCGGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCGACAACAGCTGTGTCAACATTTGTGAAATATATTTTCTATGTCAGTGATGTGCTCATGGAGGGACAATGGGAAAGGAAACCCGTCTATACATTTTACTTGGAACTTATTCGAGACTTACTTCATTTGTCGATGTACTTGTGTTTCTTTCTTGTGATTTTCATGACTTATGGTGTGCCTTTGCACTTGATCCGAGAGCTTTATGAGATCTTTAGGAATTTTAGGATTCGCATTGCAGATTACATCCGTTATAGAAAGATTACTTCAAACATGAATGATCGTTTTCCAGATGCAACGCCTGAAGAACTCAATTCAAGTGATGCAACATGCATTATTTGTCGTGAAGAGATGACAGTTGCAAAGAAACTTGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGATCTTGGTTGGAGCGACAACATACTTGTCCCACTTGTAGAGCCCTTGTTGTACCACCCGAAGGTTCAAGTACAACAAGCGGTCATCATGGAGTGCAGTCTGATGCTAACCAACAAGGAACAGGAACCACAAGTTCATCTGCACAAGGTACAAATGGCACAGGTGTTACTAATGATAATTTAAGTCATCATCAGGCTAGAGTCCGAGCTGCTGCTGCCGCTGCTTCAATATATGAGAAATCGTATGTTTATCCTTGTGCAAGCACATTAGTATGGTCTCTTGGATATGCAGTAGTTCCTCAGGTTGAAAGACCTTTGTCTGACTCTAACAATACAGAAACAAATGGAGAGCAAACTCCCAATGGACATCCACAATACTTTTCATTTCCTGGCGGCCCTTCAAACCTGTCCTTCACTCAATTTCCCCAAAATATCTTTGTTCCTTTTCAGCCACGAGAGGGCACTGGAAACAATATAGAGGAATTGGGCAGTAGTCTAAATGTATCAGGCTCACAGCTTGAAGCTCAAGAAAAATTTCTTCAACAACAGATTGAGTTCCTTCATAATCAACTGCAACTTCTCCGTAAACCAAAAGTTGAGGAAAGCTCAAGAGGAGCGAGCGAGGCAGATAAGAAAGGAAAAAGTTTAGCACCCTCACCATCGCCGTCGGTGTCATTGTCTTCATCAGATTCTCATCCGCCTGGAGAAACCGAAAGGGCGATAAGGTAA

Protein sequence

MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLAQKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYMILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTTSSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVERPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGSSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSVSLSSSDSHPPGETERAIR
Homology
BLAST of PI0008472 vs. ExPASy Swiss-Prot
Match: Q6NPT7 (ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=3702 GN=HRD1B PE=2 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 1.7e-171
Identity = 332/535 (62.06%), Postives = 393/535 (73.46%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++L+ YAG+S +AT++VIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEY+ETTPSV LLSH+RIVSF+VFL +LD L  Y+SI  LIQ+R  S+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTTSGHHGVQSDANQQGTGT 360
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +ST SG+ G   ++ QQGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 361 TSSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYP-CASTLVWSLGYAVVPQV 420
           +SS  QG++ +   ++N+S H+AR +AAA+AASIY +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 420

Query: 421 ERPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEEL 480
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 457

Query: 481 GSSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLA 534
                     +EAQ +FL+ QIE L NQL+LL KP         +  D KGKS+A
Sbjct: 481 ----------VEAQRRFLESQIEVLTNQLRLLEKP---------TTVDTKGKSVA 457

BLAST of PI0008472 vs. ExPASy Swiss-Prot
Match: Q9LW77 (ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=3702 GN=HRD1A PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 2.1e-169
Identity = 336/536 (62.69%), Postives = 398/536 (74.25%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEY+ETTPSV  LSH RIVSF+ FL ++DSLF Y+SI  LIQ+R  SVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++ +         +QQ  GT+
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ--GTS 360

Query: 361 SSSAQG---TNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQ 420
           SS  QG   ++  GV+N++LS H AR++AAA+AAS+Y KS VYP A+T+ WS G   VP 
Sbjct: 361 SSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSG---VPG 420

Query: 421 VERPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEE 480
            E+         T  +QT   H           NL                      +E 
Sbjct: 421 TEQ-------VSTEPDQTLPQH-----------NLP---------------------VEN 480

Query: 481 LGSSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLA 534
             +  N+S ++LE   K L+  +E L N+L  L   K E     A E + KGKS+A
Sbjct: 481 SHAYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPE----SAGEPENKGKSVA 488

BLAST of PI0008472 vs. ExPASy Swiss-Prot
Match: Q5Z880 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD1 PE=2 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 2.2e-158
Identity = 311/514 (60.51%), Postives = 374/514 (72.76%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++LQTYA  S +AT   +Y+AF+SR QFYPA+VYLSTSKI  VLLLN GLV MC+ WQL
Sbjct: 1   MIRLQTYAAFSLMATATAVYYAFSSREQFYPAMVYLSTSKICFVLLLNTGLVAMCVAWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            K++FLG+LREAEVERLNEQ+W+E++EILFA+TIFRQDFSV+FL MV ALLL+KALHWLA
Sbjct: 61  VKRLFLGTLREAEVERLNEQAWREVVEILFAVTIFRQDFSVSFLAMVAALLLVKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEY+ETTPSVP+LSH RIVSF++FL V+D LF  NS+ SLI  R  SV++FFSFEYM
Sbjct: 121 QKRVEYIETTPSVPMLSHARIVSFMLFLLVVDCLFLSNSLRSLIHKREASVAIFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILAT+ VSTFVKYIFYVSD+LMEGQWE+K VYTFYLELI DL+HLS+Y+ FF+ IF+ YG
Sbjct: 181 ILATSTVSTFVKYIFYVSDMLMEGQWEKKAVYTFYLELISDLVHLSLYMLFFIAIFLNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYE FRNFRIRIADY+RYRKITSNMN+RFPDAT +ELN+SDATCIICREEMT
Sbjct: 241 VPLHLIRELYETFRNFRIRIADYVRYRKITSNMNERFPDATADELNASDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQ-QGTGT 360
            AKKL+CGHLFHVHCLRSWLERQHTCPTCRA ++PP+   T +  HGV        G GT
Sbjct: 301 TAKKLLCGHLFHVHCLRSWLERQHTCPTCRAPILPPDNGRTAARPHGVHPGVQPVPGNGT 360

Query: 361 TSSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVE 420
             S            +N+S  QA++ AAA+AAS+Y +S+ YP A+ L     Y+  PQ  
Sbjct: 361 PGSER-------AAGENISRRQAKLEAAASAASLYGRSFAYPPANNL---NRYSTPPQST 420

Query: 421 RPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGN--NIEE 480
                S    T+  Q+P GH    + P  P+              F  R    +     E
Sbjct: 421 SNGPQSGEASTS-NQSPKGHAT--ADPSAPT--------------FYARGAVSSVTTTRE 480

Query: 481 LGSSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLR 512
           L SSL       +A E  ++ QIE L  QLQ+ +
Sbjct: 481 LESSLQ------KAYENAIKSQIEMLQIQLQMFQ 481

BLAST of PI0008472 vs. ExPASy Swiss-Prot
Match: Q803I8 (E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2)

HSP 1 Score: 263.5 bits (672), Expect = 5.6e-69
Identity = 145/353 (41.08%), Postives = 208/353 (58.92%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     V++ +L +L
Sbjct: 1   MVRAALVTATSLALTGAVVAHAYFLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            +K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+ + T LL +K  HWLA
Sbjct: 61  MRKVFFGQLRAAEMEHLIERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           + RV++ME +P++  + H R++S +V L V+D LF  ++  S+I TR  SV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWVFHFRVLSLMVLLGVMDFLFVNHACHSII-TRGASVQLVFGFEYA 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           IL T  ++TF+KY  +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTTFIKYTLHTIDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 300
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPE+L ++D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEDLQATDNVCIICREEMV 300

Query: 301 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDA 353
           T AKKL C H+FH  CLRSW +RQ TCPTCR  V+     + T      Q+ A
Sbjct: 301 TGAKKLPCNHIFHSSCLRSWFQRQQTCPTCRMDVLRASQPNQTPAPPAAQAPA 352

BLAST of PI0008472 vs. ExPASy Swiss-Prot
Match: Q86TM6 (E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=2)

HSP 1 Score: 259.6 bits (662), Expect = 8.0e-68
Identity = 140/331 (42.30%), Postives = 197/331 (59.52%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M +       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     V++ +L ++
Sbjct: 1   MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKV 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
             K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+ + T LL +K  HWLA
Sbjct: 61  MGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           + RV++ME +P++  L H RIVS +  L +LD LF  ++  S++ TR  SV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSIL-TRGASVQLVFGFEYA 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           IL T  ++ F+KY+ +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 300
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPEEL + D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMV 300

Query: 301 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 331
           T AK+L C H+FH  CLRSW +RQ TCPTCR
Sbjct: 301 TGAKRLPCNHIFHTSCLRSWFQRQQTCPTCR 330

BLAST of PI0008472 vs. ExPASy TrEMBL
Match: A0A0A0LJG4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 PE=4 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.4e-302
Identity = 546/558 (97.85%), Postives = 550/558 (98.57%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSIDSLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTT+GHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGT+GTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNNTETNGEQ+PNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSV 540
           SLNVSGSQLEAQEKFLQQQIEFL NQLQLLRKPKVEESSRGASEADKKGKSLA SPS S 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540

Query: 541 SLSSSDSHPPGETERAIR 559
           SLSSSDSHPPGETERA+R
Sbjct: 541 SLSSSDSHPPGETERAVR 558

BLAST of PI0008472 vs. ExPASy TrEMBL
Match: A0A1S4E231 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo OX=3656 GN=LOC103495570 PE=4 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.4e-301
Identity = 545/558 (97.67%), Postives = 548/558 (98.21%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSIDSLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGT+GTGVTN NLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNANLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNNTETNGEQ+PNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSV 540
           SLNVSGSQLEAQEKFLQQQIEFL NQLQLLRKPKVEESSRGASE DKKGKSLA SPSPS 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPSPSA 540

Query: 541 SLSSSDSHPPGETERAIR 559
           SLSSSDSHPPGETER +R
Sbjct: 541 SLSSSDSHPPGETERVVR 558

BLAST of PI0008472 vs. ExPASy TrEMBL
Match: A0A5A7V9K3 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001010 PE=4 SV=1)

HSP 1 Score: 1042.3 bits (2694), Expect = 7.1e-301
Identity = 544/555 (98.02%), Postives = 547/555 (98.56%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSIDSLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGT+GTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNNTETNGEQ+PNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSV 540
           SLNVSGSQLEAQEKFLQQQIEFL NQLQLLRKPKVEESSRGASE DKKGKSLA SP+PS 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPAPSA 540

Query: 541 SLSSSDSHPPGETER 556
           SLSSSDSHPPGETER
Sbjct: 541 SLSSSDSHPPGETER 555

BLAST of PI0008472 vs. ExPASy TrEMBL
Match: A0A6J1J826 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita maxima OX=3661 GN=LOC111482600 PE=4 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 4.9e-270
Identity = 502/549 (91.44%), Postives = 514/549 (93.62%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGL+IMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLIIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSV+FLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVSFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSI+SLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDAT EELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATSEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST +GHHG QSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAAGHHGPQSDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGT+ TGV NDNLSHHQARVRAAAAAAS+YEKSYVYPCASTLVWS GYAVVPQVER
Sbjct: 361 SSSAQGTSDTGVANDNLSHHQARVRAAAAAASVYEKSYVYPCASTLVWSPGYAVVPQVER 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNN E      PNGHPQ FSF GGPSNLSFTQFPQN+FVPFQPREGTGNN + LGS
Sbjct: 421 PLPDSNNIE------PNGHPQQFSFTGGPSNLSFTQFPQNLFVPFQPREGTGNNTDGLGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPS--PSP 540
           S NVSG  LEAQE+FLQQQIE L NQLQ LRKP+VEESS GA EADKKGKS+A S   SP
Sbjct: 481 SQNVSGLHLEAQERFLQQQIEILQNQLQHLRKPEVEESS-GAGEADKKGKSVAFSSLSSP 540

Query: 541 SVSLSSSDS 548
           S S SSSDS
Sbjct: 541 SPSPSSSDS 542

BLAST of PI0008472 vs. ExPASy TrEMBL
Match: A0A6J1CJF5 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011717 PE=4 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 8.4e-270
Identity = 498/559 (89.09%), Postives = 518/559 (92.67%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLF+LDSLFFYNSI+ LI+TR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLSFLFILDSLFFYNSIEYLIRTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST  GHHG   DANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHG-SHDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQ ++GTGV  DNLSHHQARV+AAAAAASIYEKSYVYPCASTLVWS GYAVVPQ +R
Sbjct: 361 SSSAQDSSGTGVAGDNLSHHQARVQAAAAAASIYEKSYVYPCASTLVWSPGYAVVPQTQR 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL+DSNN E NGEQ PNG PQYF F GGP+NLSFTQFPQN+FVPFQPREG+ NN + LGS
Sbjct: 421 PLADSNNIEPNGEQAPNGQPQYFPFTGGPANLSFTQFPQNLFVPFQPREGSVNNTDGLGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSV 540
           S NVSG  LE QE+F+QQQIE L  QLQLLR PKVEES+R A EADKKGKS+A SP PS 
Sbjct: 481 SQNVSGLHLEDQERFIQQQIEMLQKQLQLLRNPKVEESTR-AGEADKKGKSVASSP-PSS 540

Query: 541 SLSSSDSHPP-GETERAIR 559
           S S S+SHPP GE ER +R
Sbjct: 541 SSSVSESHPPAGEIERMVR 556

BLAST of PI0008472 vs. NCBI nr
Match: XP_004139202.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B isoform X2 [Cucumis sativus] >KGN60852.1 hypothetical protein Csa_019293 [Cucumis sativus])

HSP 1 Score: 1045.8 bits (2703), Expect = 1.3e-301
Identity = 546/558 (97.85%), Postives = 550/558 (98.57%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSIDSLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTT+GHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGT+GTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNNTETNGEQ+PNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSV 540
           SLNVSGSQLEAQEKFLQQQIEFL NQLQLLRKPKVEESSRGASEADKKGKSLA SPS S 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSSA 540

Query: 541 SLSSSDSHPPGETERAIR 559
           SLSSSDSHPPGETERA+R
Sbjct: 541 SLSSSDSHPPGETERAVR 558

BLAST of PI0008472 vs. NCBI nr
Match: XP_008455400.1 (PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo] >XP_016902283.1 PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo])

HSP 1 Score: 1043.9 bits (2698), Expect = 5.0e-301
Identity = 545/558 (97.67%), Postives = 548/558 (98.21%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSIDSLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGT+GTGVTN NLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNANLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNNTETNGEQ+PNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSV 540
           SLNVSGSQLEAQEKFLQQQIEFL NQLQLLRKPKVEESSRGASE DKKGKSLA SPSPS 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPSPSA 540

Query: 541 SLSSSDSHPPGETERAIR 559
           SLSSSDSHPPGETER +R
Sbjct: 541 SLSSSDSHPPGETERVVR 558

BLAST of PI0008472 vs. NCBI nr
Match: KAA0063647.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo var. makuwa] >TYK18378.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo var. makuwa])

HSP 1 Score: 1042.3 bits (2694), Expect = 1.5e-300
Identity = 544/555 (98.02%), Postives = 547/555 (98.56%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSIDSLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGT+GTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DSNNTETNGEQ+PNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSV 540
           SLNVSGSQLEAQEKFLQQQIEFL NQLQLLRKPKVEESSRGASE DKKGKSLA SP+PS 
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPAPSA 540

Query: 541 SLSSSDSHPPGETER 556
           SLSSSDSHPPGETER
Sbjct: 541 SLSSSDSHPPGETER 555

BLAST of PI0008472 vs. NCBI nr
Match: XP_031737096.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B isoform X1 [Cucumis sativus])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.2e-300
Identity = 546/559 (97.67%), Postives = 550/559 (98.39%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSIDSLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQ-GTGT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTT+GHHGVQSDANQQ GTGT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQAGTGT 360

Query: 361 TSSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVE 420
           TSSSAQGT+GTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVE
Sbjct: 361 TSSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVE 420

Query: 421 RPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELG 480
           RPL DSNNTETNGEQ+PNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELG
Sbjct: 421 RPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELG 480

Query: 481 SSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPS 540
           SSLNVSGSQLEAQEKFLQQQIEFL NQLQLLRKPKVEESSRGASEADKKGKSLA SPS S
Sbjct: 481 SSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKGKSLAISPSSS 540

Query: 541 VSLSSSDSHPPGETERAIR 559
            SLSSSDSHPPGETERA+R
Sbjct: 541 ASLSSSDSHPPGETERAVR 559

BLAST of PI0008472 vs. NCBI nr
Match: XP_038889876.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida] >XP_038889877.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.9e-288
Identity = 528/556 (94.96%), Postives = 535/556 (96.22%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLFVLDSLFFYNSI+SLIQTR  SVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST  GHHGVQSDANQQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
           SSSAQGT+GTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPC STLVWSLGYAVVPQVER
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCPSTLVWSLGYAVVPQVER 420

Query: 421 PLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
           PL DS+NTETNGEQTPNGHPQYFSFP GPSNLSFTQFPQN+FVPFQPREGTGNN +ELGS
Sbjct: 421 PLPDSDNTETNGEQTPNGHPQYFSFPSGPSNLSFTQFPQNLFVPFQPREGTGNNTDELGS 480

Query: 481 SLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLAPSPSPSV 540
           SLNVSGSQLEAQEKFL QQIE L NQLQLLR+PKVEESSR A EADKKGKSLA S SP  
Sbjct: 481 SLNVSGSQLEAQEKFL-QQIEILQNQLQLLRRPKVEESSR-AGEADKKGKSLATSSSPLP 540

Query: 541 SLSSSDSHPPGETERA 557
           S SS DSHP GETERA
Sbjct: 541 SSSSLDSHPSGETERA 554

BLAST of PI0008472 vs. TAIR 10
Match: AT1G65040.2 (RING/U-box superfamily protein )

HSP 1 Score: 604.0 bits (1556), Expect = 1.2e-172
Identity = 332/535 (62.06%), Postives = 393/535 (73.46%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++L+ YAG+S +AT++VIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEY+ETTPSV LLSH+RIVSF+VFL +LD L  Y+SI  LIQ+R  S+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTTSGHHGVQSDANQQGTGT 360
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +ST SG+ G   ++ QQGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 361 TSSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYP-CASTLVWSLGYAVVPQV 420
           +SS  QG++ +   ++N+S H+AR +AAA+AASIY +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 420

Query: 421 ERPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEEL 480
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 457

Query: 481 GSSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLA 534
                     +EAQ +FL+ QIE L NQL+LL KP         +  D KGKS+A
Sbjct: 481 ----------VEAQRRFLESQIEVLTNQLRLLEKP---------TTVDTKGKSVA 457

BLAST of PI0008472 vs. TAIR 10
Match: AT3G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 597.0 bits (1538), Expect = 1.5e-170
Identity = 336/536 (62.69%), Postives = 398/536 (74.25%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEY+ETTPSV  LSH RIVSF+ FL ++DSLF Y+SI  LIQ+R  SVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++ +         +QQ  GT+
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ--GTS 360

Query: 361 SSSAQG---TNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQ 420
           SS  QG   ++  GV+N++LS H AR++AAA+AAS+Y KS VYP A+T+ WS G   VP 
Sbjct: 361 SSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSG---VPG 420

Query: 421 VERPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEE 480
            E+         T  +QT   H           NL                      +E 
Sbjct: 421 TEQ-------VSTEPDQTLPQH-----------NLP---------------------VEN 480

Query: 481 LGSSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLA 534
             +  N+S ++LE   K L+  +E L N+L  L   K E     A E + KGKS+A
Sbjct: 481 SHAYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPE----SAGEPENKGKSVA 488

BLAST of PI0008472 vs. TAIR 10
Match: AT1G65040.3 (RING/U-box superfamily protein )

HSP 1 Score: 471.9 bits (1213), Expect = 7.3e-133
Identity = 277/535 (51.78%), Postives = 331/535 (61.87%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
           M++L+ YAG+S +AT++VIYHAF+SRGQFYPA VYLSTSKINL                 
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINL----------------- 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
                                                                  LHW+A
Sbjct: 61  ------------------------------------------------------GLHWMA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLVFLFVLDSLFFYNSIDSLIQTRPGSVSLFFSFEYM 180
           QKRVEY+ETTPSV LLSH+RIVSF+VFL +LD L  Y+SI  LIQ+R  S+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTTSGHHGVQSDANQQGTGT 360
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +ST SG+ G   ++ QQGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 361 TSSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYP-CASTLVWSLGYAVVPQV 420
           +SS  QG++ +   ++N+S H+AR +AAA+AASIY +S VYP  A+TLVWS G +++PQ 
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQT 386

Query: 421 ERPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEEL 480
           E                                                           
Sbjct: 421 E----------------------------------------------------------- 386

Query: 481 GSSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLA 534
                     +EAQ +FL+ QIE L NQL+LL KP         +  D KGKS+A
Sbjct: 481 ----------VEAQRRFLESQIEVLTNQLRLLEKP---------TTVDTKGKSVA 386

BLAST of PI0008472 vs. TAIR 10
Match: AT1G65040.1 (RING/U-box superfamily protein )

HSP 1 Score: 351.3 bits (900), Expect = 1.4e-96
Identity = 200/356 (56.18%), Postives = 236/356 (66.29%), Query Frame = 0

Query: 180 MILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTY 239
           MILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM Y
Sbjct: 1   MILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNY 60

Query: 240 GVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM 299
           G+PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEM
Sbjct: 61  GLPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEM 120

Query: 300 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTTSGHHGVQSDANQQGTG 359
           T AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +ST SG+ G   ++ QQGTG
Sbjct: 121 TSAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTG 180

Query: 360 TTSSSAQGTNGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYP-CASTLVWSLGYAVVPQ 419
           T+SS  QG++ +   ++N+S H+AR +AAA+AASIY +S VYP  A+TLVWS G +++PQ
Sbjct: 181 TSSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQ 240

Query: 420 VERPLSDSNNTETNGEQTPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEE 479
            E                                                          
Sbjct: 241 TE---------------------------------------------------------- 278

Query: 480 LGSSLNVSGSQLEAQEKFLQQQIEFLHNQLQLLRKPKVEESSRGASEADKKGKSLA 534
                      +EAQ +FL+ QIE L NQL+LL KP         +  D KGKS+A
Sbjct: 301 -----------VEAQRRFLESQIEVLTNQLRLLEKP---------TTVDTKGKSVA 278

BLAST of PI0008472 vs. TAIR 10
Match: AT4G25230.1 (RPM1 interacting protein 2 )

HSP 1 Score: 97.1 bits (240), Expect = 4.9e-20
Identity = 98/343 (28.57%), Postives = 155/343 (45.19%), Query Frame = 0

Query: 25  SRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQLTKKIFLGSLREAEVERLNEQSWKE 84
           S G    AL  L  S   + LL N  L +  +L    K +F G L + E ++L E+    
Sbjct: 42  SLGDSEHALELLLGSYFTIALLTNFVLNVYILLVLSLKTLFFGDLYDVETKKLVERLANY 101

Query: 85  L------MEILFAITIFRQDFSVAFLGMVTALLLIKALHWLAQKRVEYMETTPSVPLLSH 144
           +      + ++   TIF+    V +   +T L  +K    LA+ R+E +  +PS    ++
Sbjct: 102 IIYKGTFLPLVIPPTIFQ---GVLWTVWLTVLCTLKMFQALARDRLERLNASPSSTPWTY 161

Query: 145 IRIVSFLVFLFVLD------SLFFYNSIDS----LIQTRPGSVSLFFSFEYMILATTAVS 204
            R+ S L  +  +D      SL  YN+I S    L+   P S++       +I     + 
Sbjct: 162 FRVYSVLFLVLSVDMFWIKLSLMTYNTIGSAVYLLLLFEPCSIAFETLQALLIHGFQLLD 221

Query: 205 TFVKYIF----------YVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMT 264
            ++ ++           ++  +      E K +    L    D+  L M L  +L I+  
Sbjct: 222 MWINHLAVKNSDCQRSKFIDSMTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWL 281

Query: 265 YGVPLHLIRELYEIFRNFRI-------RIADYIRYRKITSNMNDRFPDATPEELNSSDAT 324
           +G+  HL+  +  +F N R        RI  YI+ R     ++   PDAT EEL + D  
Sbjct: 282 HGIAFHLVDAV--LFLNIRALLSAILKRIKGYIKLRIALGALHAALPDATSEELRAYDDE 341

Query: 325 CIICREEMTVAKKLVCGHLFHVHCLRSWLER----QHTCPTCR 331
           C ICRE M  AK+L C HLFH+ CLRSWL++     ++CPTCR
Sbjct: 342 CAICREPMAKAKRLHCNHLFHLGCLRSWLDQGLNEVYSCPTCR 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NPT71.7e-17162.06ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=370... [more]
Q9LW772.1e-16962.69ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=370... [more]
Q5Z8802.2e-15860.51ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica... [more]
Q803I85.6e-6941.08E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2[more]
Q86TM68.0e-6842.30E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LJG46.4e-30297.85RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 P... [more]
A0A1S4E2312.4e-30197.67ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo OX=3656 G... [more]
A0A5A7V9K37.1e-30198.02ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. maku... [more]
A0A6J1J8264.9e-27091.44ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita maxima OX=36... [more]
A0A6J1CJF58.4e-27089.09ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X1 OS=Momordica c... [more]
Match NameE-valueIdentityDescription
XP_004139202.11.3e-30197.85ERAD-associated E3 ubiquitin-protein ligase HRD1B isoform X2 [Cucumis sativus] >... [more]
XP_008455400.15.0e-30197.67PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo]... [more]
KAA0063647.11.5e-30098.02ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucumis melo var. makuwa... [more]
XP_031737096.13.2e-30097.67ERAD-associated E3 ubiquitin-protein ligase HRD1B isoform X1 [Cucumis sativus][more]
XP_038889876.14.9e-28894.96ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Benincasa hispida] >XP_0... [more]
Match NameE-valueIdentityDescription
AT1G65040.21.2e-17262.06RING/U-box superfamily protein [more]
AT3G16090.11.5e-17062.69RING/U-box superfamily protein [more]
AT1G65040.37.3e-13351.78RING/U-box superfamily protein [more]
AT1G65040.11.4e-9656.18RING/U-box superfamily protein [more]
AT4G25230.14.9e-2028.57RPM1 interacting protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 486..506
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 510..527
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 337..376
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 534..550
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 510..558
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 1..457
NoneNo IPR availablePANTHERPTHR22763:SF174SUBFAMILY NOT NAMEDcoord: 1..457
NoneNo IPR availableCDDcd16479RING-H2_synoviolincoord: 289..330
e-value: 1.7437E-27
score: 102.376
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 272..336
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 292..329
e-value: 2.0E-5
score: 34.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 291..330
e-value: 1.2E-10
score: 41.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 292..330
score: 12.415513
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 264..336
e-value: 5.4E-23
score: 82.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0008472.1PI0008472.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity