PI0008420 (gene) Melon (PI 482460) v1

Overview
NamePI0008420
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPyruvate dehydrogenase E1 component subunit alpha
Locationchr11: 1898707 .. 1901112 (+)
RNA-Seq ExpressionPI0008420
SyntenyPI0008420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTTAACTCCACCAGATCCAATGACAAACCCCTTTTCTTTGATCCCTTTAGATCCACCTCCAAGTTTCTTGGATCTCGATTTCGTCTCCCTTCTCTCTCTAAATCCAATACTCGTTGTCGATCTTCCCCTCCTGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTCTAGATTATGAATTTGTTGTATTGATTGATTTGTTTTTTGGGTTTTTAATGTCCTGGAAATCATGGGGGGATTTGGTAATGTTGCATGAAATTTCATTTTCTATGCCCGCATGGAATTATTCTAAGCTTTAACGATTTTGCTTTTGTTGGTTTTGGTTTCCCTGTTGTTATTTTCTTTTGGAAATTTGGATGGTTTGATTGAGATTGTCTTTATGAAATTGGAGGTTATATTGTTCTTTTGTCTCATCAGTGGAATTAAATTTTTACTCTACTGGAAGCTTTTGTGTGCATTGCTTTGCTGCATGAAGCACCTTGGTCTAAAAGTTCTTCCCTTTTTTGGTTCCTTTTATTGTCTCCACACAGCTGATTACAAAAGAGGAGGGTTTGGTACTCTATGAGGACATGATATTAGGCAGAGAATTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACTGGGTTCATCAAGCTTCTTACAAAGAGGGATACCGTCGTAAGCACTTATCGTGATCACGTCCATGCTCTCAGTAAGGGAGTTCCATCTCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGGTGCTGCCGTGGCCAAGGTGGCTCAATGCATATGTTCTCAAAGAACATAATCTGATAGGGGGCTTTGCCTTCATTGGAGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTGACATTGGCATTTTTCGGGGATGGAACTTGCAACAACGGCCAGTTCTTCGAGTGCTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCGCATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAAAAGGGTCCTGCATTTGGAATGCCTGGTGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGAGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGAAGAGGAGAAGGACCGACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCTCTGGCTGATCCAGATGAACTCCGTGACCCTGGTTAGTGCTCTTAAATTCATACCTACAATTGGTTCTGTCATATTCTAAATATGCTTTTGCTTGGCCTTGTTGACATTTATTATTACGGCTAGTCAAAGTTCCTTAATTCATTTCTCATGAATATGATAGCTGATCTCTTAATCCGTACTCCATCATAGCCATTTAAGGATAATTCATTCAGGGTGTTATGAGCTGCACCGCTAGAAATTCACTCATTAGTTAACTCAAAAAATTTTCTCATTTGTTCATCTATTATCTTTAGACTCAATTAGATTTTTTTTTTTTAATTTTTTTTTATTTCTTTATTTTTTAAATGGAACCAACTAACTTTCATTGAGAAAAAAAATGAAAGAATACAGGGCATGCTTCCACCAAAGCACCCCTTCTAAAGAAAGGATTTCCAACTAAGTAAGATATTACTTAGAGAATAATTAAAAAAATAAAAAACTTTGAAACCGAAGCATAAAGCGAGACATGAAAACTCATGAGGAATCAAACCTCACTCGGGATTCTGTTCAAACCTCTAAACACTCTAGTGTTCCTTTTTCCCAAATATCCCACAATAAAGCTGATTCTTCATAGATGATAGAAATCGATGTCCCCATGAGGTTCTTCATTGTACTTTGCTTACTTACACACACAAATGTAGTAGTTTCTTAGCCAAGTATAGTAAACTCCGGAAAGTACAAAGGATTAAGAATTCCCTGATTTAACCATTGCAGATGAGAAGGCTCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAGTACTTGCTGGAGAATAAACTAGCCAATGAACAGGAGCTAAAGGCAATCAAGGATAAGATAGTTGAGGTGGTTGAGGATGCAGTTGAATTTGCAGATGAAAGCCCCCTTCCAGCAAGAAGCCAGTTATTGGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGGAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGAACTGCACATGTCTAA

mRNA sequence

ATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTTAACTCCACCAGATCCAATGACAAACCCCTTTTCTTTGATCCCTTTAGATCCACCTCCAAGTTTCTTGGATCTCGATTTCGTCTCCCTTCTCTCTCTAAATCCAATACTCGTTGTCGATCTTCCCCTCCTGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGCTGATTACAAAAGAGGAGGGTTTGGTACTCTATGAGGACATGATATTAGGCAGAGAATTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACTGGGTTCATCAAGCTTCTTACAAAGAGGGATACCGTCGTAAGCACTTATCGTGATCACGTCCATGCTCTCAGTAAGGGAGTTCCATCTCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGGTGCTGCCAACATAATCTGATAGGGGGCTTTGCCTTCATTGGAGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTGACATTGGCATTTTTCGGGGATGGAACTTGCAACAACGGCCAGTTCTTCGAGTGCTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCGCATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAAAAGGGTCCTGCATTTGGAATGCCTGGTGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGAGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGAAGAGGAGAAGGACCGACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCTCTGGCTGATCCAGATGAACTCCGTGACCCTGATGAGAAGGCTCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAGTACTTGCTGGAGAATAAACTAGCCAATGAACAGGAGCTAAAGGCAATCAAGGATAAGATAGTTGAGGTGGTTGAGGATGCAGTTGAATTTGCAGATGAAAGCCCCCTTCCAGCAAGAAGCCAGTTATTGGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGGAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGAACTGCACATGTCTAA

Coding sequence (CDS)

ATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTTAACTCCACCAGATCCAATGACAAACCCCTTTTCTTTGATCCCTTTAGATCCACCTCCAAGTTTCTTGGATCTCGATTTCGTCTCCCTTCTCTCTCTAAATCCAATACTCGTTGTCGATCTTCCCCTCCTGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGCTGATTACAAAAGAGGAGGGTTTGGTACTCTATGAGGACATGATATTAGGCAGAGAATTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCCACTGGGTTCATCAAGCTTCTTACAAAGAGGGATACCGTCGTAAGCACTTATCGTGATCACGTCCATGCTCTCAGTAAGGGAGTTCCATCTCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGGTGCTGCCAACATAATCTGATAGGGGGCTTTGCCTTCATTGGAGAAGGCATACCAGTGGCCACTGGTGCTGCATTCACATCTAAATACAAGAGAGAAGTTTTAAAAGAGGCAGACTGTGAAGATGTGACATTGGCATTTTTCGGGGATGGAACTTGCAACAACGGCCAGTTCTTCGAGTGCTTGAACATGGCAGCTCTGTGGAAATTGCCAATTGTATTTGTTGTGGAGAATAATCTTTGGGCAATTGGAATGTCGCATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAAAAGGGTCCTGCATTTGGAATGCCTGGTGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGAGAGGTTGCAAAGGAGGCAATTGGAAGAGCCAGAAGAGGAGAAGGACCGACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCTCTGGCTGATCCAGATGAACTCCGTGACCCTGATGAGAAGGCTCGTTATGCTGCTAGAGATCCCATTGCAGCTTTGAAGAAGTACTTGCTGGAGAATAAACTAGCCAATGAACAGGAGCTAAAGGCAATCAAGGATAAGATAGTTGAGGTGGTTGAGGATGCAGTTGAATTTGCAGATGAAAGCCCCCTTCCAGCAAGAAGCCAGTTATTGGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGGAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGAACTGCACATGTCTAA

Protein sequence

MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVAVSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCQHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKLANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGTAHV
Homology
BLAST of PI0008420 vs. ExPASy Swiss-Prot
Match: O24457 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PDH-E1 ALPHA PE=2 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 2.5e-183
Identity = 329/425 (77.41%), Postives = 360/425 (84.71%), Query Frame = 0

Query: 8   KILQPLPLNSTRSNDKPLFFDPFR--STSKFLGS--RFRLPSLSKSNTRCRSSPPVAVSD 67
           K+   +PL+ +  N   L   P R    S FLGS     L  L+ SN   R SP V+V +
Sbjct: 9   KLTATVPLHGSHENRLLL---PIRLAPPSSFLGSTRSLSLRRLNHSNA-TRRSPVVSVQE 68

Query: 68  VVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVS 127
           VVKEK+   +++LLITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVS
Sbjct: 69  VVKEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVS 128

Query: 128 TGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------QHN 187
           TGFIKLLTK D+VVSTYRDHVHALSKGV +R VMSELFGK TGCC            +HN
Sbjct: 129 TGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHN 188

Query: 188 LIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAA 247
           ++GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC+DVT+AFFGDGTCNNGQFFECLNMAA
Sbjct: 189 MLGGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAA 248

Query: 248 LWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAI 307
           L+KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+
Sbjct: 249 LYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAV 308

Query: 308 GRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKLANE 367
            RARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPIAALKKYL+ENKLA E
Sbjct: 309 TRARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKE 368

Query: 368 QELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTE 417
            ELK+I+ KI E+VE+AVEFAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPKFTE
Sbjct: 369 AELKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPKFTE 428

BLAST of PI0008420 vs. ExPASy Swiss-Prot
Match: Q7XTJ3 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0119400 PE=2 SV=2)

HSP 1 Score: 600.1 bits (1546), Expect = 1.9e-170
Identity = 309/431 (71.69%), Postives = 345/431 (80.05%), Query Frame = 0

Query: 2   SFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVAV 61
           SF+++ K L P+   S      PL   P  +++     R       K   R R++  V+ 
Sbjct: 6   SFTAAAKFLAPVSARSAGDYKPPL---PLPASASLRPGR-------KPAPRLRTALAVS- 65

Query: 62  SDVVKEKKLKP--SSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQ 121
           SDV+   K  P  +++  +T+EE L LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQ
Sbjct: 66  SDVLPGNKAAPAAAAHSAVTREEALELYEDMVLGRIFEDMCAQMYYRGKMFGFVHLYNGQ 125

Query: 122 EAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCQ---------- 181
           EAVSTGFIKLL + D VVSTYRDHVHALSKGVP+R VM+ELFGK TGCC+          
Sbjct: 126 EAVSTGFIKLLNQADCVVSTYRDHVHALSKGVPARSVMAELFGKATGCCRGQGGSMHMFS 185

Query: 182 --HNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEA--DCEDVTLAFFGDGTCNNGQFFE 241
             HNL+GGFAFIGEGIPVATGAAF +KY+ EVLK++  D  DVTLAFFGDGTCNNGQFFE
Sbjct: 186 EPHNLLGGFAFIGEGIPVATGAAFAAKYRHEVLKQSSPDGLDVTLAFFGDGTCNNGQFFE 245

Query: 242 CLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVRE 301
           CLNMA LWKLPIVFVVENNLWAIGMSHLRATSDPEI+KKGPAFGMPGVHVDGMDVLKVRE
Sbjct: 246 CLNMAQLWKLPIVFVVENNLWAIGMSHLRATSDPEIYKKGPAFGMPGVHVDGMDVLKVRE 305

Query: 302 VAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLE 361
           VAKEAI RARRGEGPTLVECETYRFRGHSLADPDELR PDEK+ YAARDPI ALKKY++E
Sbjct: 306 VAKEAIERARRGEGPTLVECETYRFRGHSLADPDELRRPDEKSHYAARDPITALKKYIIE 365

Query: 362 NKLANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCE 417
             LA E ELK+I+ KI +VVE+AVEFAD SPLP RSQLLENVF+DPKGFGIGPDGKYRCE
Sbjct: 366 QNLATESELKSIEKKIDDVVEEAVEFADASPLPPRSQLLENVFSDPKGFGIGPDGKYRCE 425

BLAST of PI0008420 vs. ExPASy Swiss-Prot
Match: Q1XDM0 (Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 GN=pdhA PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.1e-121
Identity = 217/330 (65.76%), Postives = 253/330 (76.67%), Query Frame = 0

Query: 73  SSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTK 132
           S+ L + K   LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG IKLL  
Sbjct: 16  STGLNLNKSNLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGVIKLLNP 75

Query: 133 RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCQ------------HNLIGGFAFIG 192
            D V STYRDHVHALSKGVPS+ VM+ELFGK TGC +            HN +GGFAFI 
Sbjct: 76  TDYVCSTYRDHVHALSKGVPSKNVMAELFGKETGCSKGRGGSMHIFSAPHNFLGGFAFIA 135

Query: 193 EGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFV 252
           EGIPVATGAAF S Y+++VLKE +   VT  FFGDGT NNGQFFECLNMA LWKLPI+FV
Sbjct: 136 EGIPVATGAAFQSIYRQQVLKETEDLRVTACFFGDGTTNNGQFFECLNMAVLWKLPIIFV 195

Query: 253 VENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGP 312
           VENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+ AK+A+ RAR+G+GP
Sbjct: 196 VENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQAAKQAVQRARQGDGP 255

Query: 313 TLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKLANEQELKAIKDK 372
           TL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKKY+L+N++AN  EL  I++ 
Sbjct: 256 TLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKYILDNEIANIGELNEIQNA 315

Query: 373 IVEVVEDAVEFADESPLPARSQLLENVFAD 391
           +   +E AV+FA  SP P  S+L   +FAD
Sbjct: 316 VKTELEQAVKFAISSPEPNMSELKRYLFAD 345

BLAST of PI0008420 vs. ExPASy Swiss-Prot
Match: P51267 (Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 GN=pdhA PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 6.8e-120
Identity = 216/336 (64.29%), Postives = 256/336 (76.19%), Query Frame = 0

Query: 67  EKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGF 126
           E  L   + + +TK + LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG 
Sbjct: 8   ELPLTNCNQINLTKHKLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGV 67

Query: 127 IKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCQ------------HNLIG 186
           IKLL  +D V STYRDHVHALSKGVPS+ VM+ELFGK TGC +            HN +G
Sbjct: 68  IKLLDSKDYVCSTYRDHVHALSKGVPSQNVMAELFGKETGCSRGRGGSMHIFSAPHNFLG 127

Query: 187 GFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWK 246
           GFAFI EGIPVATGAAF S Y+++VLKE     VT  FFGDGT NNGQFFECLNMA LWK
Sbjct: 128 GFAFIAEGIPVATGAAFQSIYRQQVLKEPGELRVTACFFGDGTTNNGQFFECLNMAVLWK 187

Query: 247 LPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRA 306
           LPI+FVVENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+VA++A+ RA
Sbjct: 188 LPIIFVVENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQVAEKAVERA 247

Query: 307 RRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKLANEQEL 366
           R+G+GPTL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKK++L+N++A+  EL
Sbjct: 248 RQGQGPTLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKHILDNQIASSDEL 307

Query: 367 KAIKDKIVEVVEDAVEFADESPLPARSQLLENVFAD 391
             I+  +   +E +VEFA  SP P  S+L   +FAD
Sbjct: 308 NDIQSSVKIDLEQSVEFAMSSPEPNISELKRYLFAD 343

BLAST of PI0008420 vs. ExPASy Swiss-Prot
Match: Q92IS3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=pdhA PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.9e-69
Identity = 129/336 (38.39%), Postives = 200/336 (59.52%), Query Frame = 0

Query: 65  VKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVST 124
           +K  K KP      TKEE +  ++DM+L R FE+ C Q+Y  G++ GF HLY GQEAV +
Sbjct: 3   IKLGKYKP------TKEEYIKSFKDMLLLRRFEEKCGQLYGMGEIGGFCHLYIGQEAVIS 62

Query: 125 GFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCQ------------HNL 184
               +  K D+ +++YRDH H +  G   + V++EL G+ TGC +            +  
Sbjct: 63  AIDMVKQKGDSTITSYRDHAHIILAGTEPKYVLAELMGRATGCSKGKGGSMHLFNVPNKF 122

Query: 185 IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAAL 244
            GG   +G  +P+ TG AF  KY        D  ++   F GDG  N GQ +E  NMAAL
Sbjct: 123 YGGHGIVGAQVPIGTGLAFVEKYN-------DTHNICFTFLGDGAVNQGQVYEAFNMAAL 182

Query: 245 WKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIG 304
           W LP+V+++ENN +++G S  R+T   +++KKG +FG+ G  +DGMD  ++ + +K+A  
Sbjct: 183 WGLPVVYIIENNEYSMGTSVARSTFMRDLYKKGASFGIKGFQLDGMDFEEMYDGSKQAAE 242

Query: 305 RARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKLANEQ 364
             R    P ++E +TYR+RGHS++DP + R  +E  +Y  RDP+  ++K +L+NK   E 
Sbjct: 243 YVRENSFPLILEVKTYRYRGHSMSDPAKYRSKEEVEQYKERDPLVIIRKTILDNKYVTEA 302

Query: 365 ELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVF 389
           +LKAI+  + E+V++AVEF++ SPLP   +L   V+
Sbjct: 303 DLKAIEQSVKEIVKEAVEFSENSPLPDEGELYTQVY 325

BLAST of PI0008420 vs. ExPASy TrEMBL
Match: A0A5A7TEC1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002450 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 3.8e-235
Identity = 408/428 (95.33%), Postives = 413/428 (96.50%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MSFSSSLKILQPLPLNSTRSNDKPL FDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           ANEQELKAIKDKIVEVVEDAV+FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. ExPASy TrEMBL
Match: A0A1S3CB66 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC103498837 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 3.8e-235
Identity = 408/428 (95.33%), Postives = 413/428 (96.50%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MSFSSSLKILQPLPLNSTRSNDKPL FDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           ANEQELKAIKDKIVEVVEDAV+FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. ExPASy TrEMBL
Match: A0A0A0KFF3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_6G095870 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.3e-234
Identity = 407/428 (95.09%), Postives = 413/428 (96.50%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MSFSSSLKILQPLPLNSTRSNDKPL FDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           ANEQELKAIKDKIVEVVE+AV+FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. ExPASy TrEMBL
Match: A0A6J1JV75 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111488634 PE=4 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.4e-221
Identity = 387/428 (90.42%), Postives = 399/428 (93.22%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MSFS+S KILQPL LNS RSN+KPLFFDPFRSTS FLGSRFR PS+SKS TRCRSSP VA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLV+YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKYL+E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           A+EQELKAI+ +I EVVE+AVEFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. ExPASy TrEMBL
Match: A0A6J1EPB1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111436336 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 5.4e-221
Identity = 387/428 (90.42%), Postives = 398/428 (92.99%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MS S+S KILQPL LNS RS +KPLFFDPFRSTS FLGSRFR PS+SKS TRCRSSP VA
Sbjct: 1   MSLSASPKILQPLLLNSPRSTEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLVLYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKYL+E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           A+EQELKAI+ +I EVVE+AVEFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. NCBI nr
Match: XP_008459830.1 (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis melo] >KAA0039805.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa] >TYK24692.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa])

HSP 1 Score: 823.5 bits (2126), Expect = 7.9e-235
Identity = 408/428 (95.33%), Postives = 413/428 (96.50%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MSFSSSLKILQPLPLNSTRSNDKPL FDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           ANEQELKAIKDKIVEVVEDAV+FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. NCBI nr
Match: XP_004140530.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sativus] >KGN46471.1 hypothetical protein Csa_005756 [Cucumis sativus])

HSP 1 Score: 820.5 bits (2118), Expect = 6.7e-234
Identity = 407/428 (95.09%), Postives = 413/428 (96.50%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MSFSSSLKILQPLPLNSTRSNDKPL FDPFRSTSKFLGSRFRLPSLSKSNTRCRSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKY+LENKL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           ANEQELKAIKDKIVEVVE+AV+FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. NCBI nr
Match: XP_038875269.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hispida])

HSP 1 Score: 815.1 bits (2104), Expect = 2.8e-232
Identity = 404/428 (94.39%), Postives = 410/428 (95.79%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MSFSSSLKILQPLPLNSTRSN+KPLFFDPFRS+SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKYLLEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           A+EQELK IKDKIVEVVEDAV FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. NCBI nr
Match: XP_022992260.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 778.5 bits (2009), Expect = 2.9e-221
Identity = 387/428 (90.42%), Postives = 399/428 (93.22%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MSFS+S KILQPL LNS RSN+KPLFFDPFRSTS FLGSRFR PS+SKS TRCRSSP VA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLV+YEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKYL+E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           A+EQELKAI+ +I EVVE+AVEFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. NCBI nr
Match: XP_022929856.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 776.5 bits (2004), Expect = 1.1e-220
Identity = 387/428 (90.42%), Postives = 398/428 (92.99%), Query Frame = 0

Query: 1   MSFSSSLKILQPLPLNSTRSNDKPLFFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPPVA 60
           MS S+S KILQPL LNS RS +KPLFFDPFRSTS FLGSRFR PS+SKS TRCRSSP VA
Sbjct: 1   MSLSASPKILQPLLLNSPRSTEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLVLYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------ 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCC            
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           +HN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPIAALKKYL+E  L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 417
           A+EQELKAI+ +I EVVE+AVEFADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

BLAST of PI0008420 vs. TAIR 10
Match: AT1G01090.1 (pyruvate dehydrogenase E1 alpha )

HSP 1 Score: 642.9 bits (1657), Expect = 1.8e-184
Identity = 329/425 (77.41%), Postives = 360/425 (84.71%), Query Frame = 0

Query: 8   KILQPLPLNSTRSNDKPLFFDPFR--STSKFLGS--RFRLPSLSKSNTRCRSSPPVAVSD 67
           K+   +PL+ +  N   L   P R    S FLGS     L  L+ SN   R SP V+V +
Sbjct: 9   KLTATVPLHGSHENRLLL---PIRLAPPSSFLGSTRSLSLRRLNHSNA-TRRSPVVSVQE 68

Query: 68  VVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVS 127
           VVKEK+   +++LLITKEEGL LYEDMILGR FEDMCAQMYYRGKMFGFVHLYNGQEAVS
Sbjct: 69  VVKEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVS 128

Query: 128 TGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------QHN 187
           TGFIKLLTK D+VVSTYRDHVHALSKGV +R VMSELFGK TGCC            +HN
Sbjct: 129 TGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHN 188

Query: 188 LIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAA 247
           ++GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC+DVT+AFFGDGTCNNGQFFECLNMAA
Sbjct: 189 MLGGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAA 248

Query: 248 LWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAI 307
           L+KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+
Sbjct: 249 LYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAV 308

Query: 308 GRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKLANE 367
            RARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPIAALKKYL+ENKLA E
Sbjct: 309 TRARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKE 368

Query: 368 QELKAIKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTE 417
            ELK+I+ KI E+VE+AVEFAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPKFTE
Sbjct: 369 AELKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPKFTE 428

BLAST of PI0008420 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 230.3 bits (586), Expect = 2.8e-60
Identity = 135/348 (38.79%), Postives = 192/348 (55.17%), Query Frame = 0

Query: 72  PSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLL 131
           PS ++  + +E L  +  M L R  E + A   Y+ K+  GF HLY+GQEAV+ G    +
Sbjct: 48  PSRSVESSSQELLDFFRTMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAI 107

Query: 132 TKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCC------------QHNLIGGFAF 191
           TK+D +++ YRDH   L +G    EV SEL G+  GC             + +  GG   
Sbjct: 108 TKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGI 167

Query: 192 IGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIV 251
           +G  +P+  G AF  KY +E       E VT A +GDG  N GQ FE LN++ALW LP +
Sbjct: 168 VGAQVPLGCGIAFAQKYNKE-------EAVTFALYGDGAANQGQLFEALNISALWDLPAI 227

Query: 252 FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGE 311
            V ENN + +G +  RA   P  +K+G    +PG+ VDGMD   V++  K A   A   +
Sbjct: 228 LVCENNHYGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQHALE-K 287

Query: 312 GPTLVECETYRFRGHSLADP-DELRDPDE-KARYAARDPIAALKKYLLENKLANEQELKA 371
           GP ++E +TYR+ GHS++DP    R  DE       RDPI  +KK +L + LA E+ELK 
Sbjct: 288 GPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKD 347

Query: 372 IKDKIVEVVEDAVEFADESPLPARSQLLENVFADPKGFG---IGPDGK 402
           ++ +I + V+DA+  A + P+P  S+L  NV+   KGFG    GPD K
Sbjct: 348 MEKEIRKEVDDAIAKAKDCPMPEPSELFTNVYV--KGFGTESFGPDRK 382

BLAST of PI0008420 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 222.6 bits (566), Expect = 5.8e-58
Identity = 138/377 (36.60%), Postives = 201/377 (53.32%), Query Frame = 0

Query: 43  LPSLSKSNTRCRSSPPVAVSDVVKEKKL--KPSSNLLITKEEGLVLYEDMILGREFEDMC 102
           LPS  + +    SSP    + V     L   PS ++  + EE L  + DM   R  E + 
Sbjct: 21  LPSSIRRHVSTDSSPITIETAVPFTSHLCESPSRSVETSSEEILAFFRDMARMRRME-IA 80

Query: 103 AQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSE 162
           A   Y+ K+  GF HLY+GQEA++ G    +TK+D ++++YRDH   + +G    +  SE
Sbjct: 81  ADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSE 140

Query: 163 LFGKTTGCC------------QHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCED 222
           L G+ TGC               +  GG   +G  IP+  G AF  KY ++       E 
Sbjct: 141 LMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQKYNKD-------EA 200

Query: 223 VTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPA 282
           VT A +GDG  N GQ FE LN++ALW LP + V ENN + +G +  R+   P  +K+G  
Sbjct: 201 VTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYFKRGDY 260

Query: 283 FGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADP-DELRDPDE 342
             +PG+ VDGMD L V++  K A   A +  GP ++E +TYR+ GHS++DP    R  DE
Sbjct: 261 --VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILEMDTYRYHGHSMSDPGSTYRTRDE 320

Query: 343 -KARYAARDPIAALKKYLLENKLANEQELKAIKDKIVEVVEDAVEFADESPLPARSQLLE 402
                  RDPI  ++K LL + +A E+ELK ++ +I + V+DAV  A ESP+P  S+L  
Sbjct: 321 ISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESPIPDASELFT 380

BLAST of PI0008420 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-24
Identity = 81/327 (24.77%), Postives = 153/327 (46.79%), Query Frame = 0

Query: 78  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 137
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 125 VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 184

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTT------------GCCQHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +            G  + N     A I   +P 
Sbjct: 185 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 244

Query: 198 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  ++       +   + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 245 AVGAAYSLKMDKK-------DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 304

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 305 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 364

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIAALKKYLLENKLANEQELKAIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P++  + ++  N   +++    ++ +I +
Sbjct: 365 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 424

Query: 378 VVEDAVEFADESPLPARSQLLENVFAD 391
            + +A+  A+++  P     L+N+F+D
Sbjct: 425 EMLEALRVAEKTEKPN----LQNMFSD 439

BLAST of PI0008420 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-24
Identity = 81/327 (24.77%), Postives = 153/327 (46.79%), Query Frame = 0

Query: 78  ITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 137
           +++E  + +Y DM+  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 54  VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 113

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTT------------GCCQHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +            G  + N     A I   +P 
Sbjct: 114 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 173

Query: 198 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  ++       +   + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 174 AVGAAYSLKMDKK-------DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 233

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 234 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 293

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIAALKKYLLENKLANEQELKAIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P++  + ++  N   +++    ++ +I +
Sbjct: 294 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 353

Query: 378 VVEDAVEFADESPLPARSQLLENVFAD 391
            + +A+  A+++  P     L+N+F+D
Sbjct: 354 EMLEALRVAEKTEKPN----LQNMFSD 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O244572.5e-18377.41Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsi... [more]
Q7XTJ31.9e-17071.69Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sati... [more]
Q1XDM02.1e-12165.76Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 G... [more]
P512676.8e-12064.29Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 G... [more]
Q92IS31.9e-6938.39Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia conorii (strain ... [more]
Match NameE-valueIdentityDescription
A0A5A7TEC13.8e-23595.33Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX... [more]
A0A1S3CB663.8e-23595.33Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A0A0KFF33.3e-23495.09Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=... [more]
A0A6J1JV751.4e-22190.42Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1EPB15.4e-22190.42Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
XP_008459830.17.9e-23595.33PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [C... [more]
XP_004140530.16.7e-23495.09pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sati... [more]
XP_038875269.12.8e-23294.39pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hi... [more]
XP_022992260.12.9e-22190.42pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita ma... [more]
XP_022929856.11.1e-22090.42pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita mo... [more]
Match NameE-valueIdentityDescription
AT1G01090.11.8e-18477.41pyruvate dehydrogenase E1 alpha [more]
AT1G59900.12.8e-6038.79pyruvate dehydrogenase complex E1 alpha subunit [more]
AT1G24180.15.8e-5836.60Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.11.4e-2424.77Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.21.4e-2424.77Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.970coord: 48..398
e-value: 3.1E-112
score: 377.2
NoneNo IPR availablePANTHERPTHR11516PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLARcoord: 5..416
NoneNo IPR availablePANTHERPTHR11516:SF58PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHAcoord: 5..416
NoneNo IPR availableCDDcd02000TPP_E1_PDC_ADC_BCADCcoord: 86..372
e-value: 8.7136E-143
score: 406.881
IPR001017Dehydrogenase, E1 componentPFAMPF00676E1_dhcoord: 89..378
e-value: 4.1E-74
score: 249.2
IPR017597Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup yTIGRFAMTIGR03182TIGR03182coord: 81..389
e-value: 8.1E-124
score: 410.6
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 76..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0008420.1PI0008420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006086 acetyl-CoA biosynthetic process from pyruvate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity
molecular_function GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor