PI0008307 (gene) Melon (PI 482460) v1

Overview


Name	PI0008307
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	transcription factor IIIB 90 kDa subunit
Location	chr07: 19775126 .. 19779792 (-)
RNA-Seq Expression	PI0008307
Synteny	PI0008307

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGTGGTGTCACCACGAGTGGGGTTAAATATTGAGATTGTAAGGTAGCTTAGAGCCGATGAGCTCAACTTGCTTATTTCCTCGCCAATGTGAAATGGGAGCAAGCTCGACCTAATCTGCAAGGAGTTAAGGCTTCCCACCATGGGACATGTGGTCTTTTAGGTTTCCTTGCCCCTGCCTTATAGATAATCATAATTTTAGATATATAATTAAATTTAACATTTTTATCCACTGACTTAAGTTTTTAGGTCAAAGTATGTTATTTGAATGGTATTTGAGTGGATCGTTCAAGGAGATTTTGTGTTCAAGTCTTTGCATAGTTAATAATTTCCTCCTTATTGATATTAATTTTTAGTTATTGGGCATTTTCAGATTTCGAGCTCACAAGTGAGAGGAGTGTGTTAGATACATACTTAAATTTTAACTCTACCCACTAGCTTAAGATTTTAGGTCAAATTGTGCGTGATTTAAAACATACTTTACATATTCAACTTAAATGTACATAAACTAAATATTAAGGCCTTAGTTGGTAACCATTTTGTTTTTTGTTTTTTGGTTTTTGAAAATTAAGTCTATTTCTTCTCAATGTCTTATAATGATTTGTATCTTTCTTAAATTACAATGGTTGATTCTTAGTCAAATTTCAAATTCCTAAAACAACTTTTTTAAAGTTATTGTTTTTAGTATTCAAAATCTGGCTTGGTTTTTTAAATTATTGATAAAATGTAGATAACAAAGGAAGAAAGTTGAAGGTGAAAGGAATGTTTATAGACTTAATTTTCAAAAACAAAAACAAAAACAAAATGGTTATCAAATGAAACGTAGGTTATATTTATGTTTTCTATCTTCAGAAAATAATTGTTTGTATTATAAATATCATTAAAAATAGATGTCCTATTATGCTTGGTGTCCTTTGCTATGTGTCACTCCCCTCCACATCCAATTCTTTTGTCATGTGTCTTGAAGATACCAACTCTTAGTAAATCCATCTCATGCTTGTCAAATTGCTATAAATAGTGACATCTTGTGGGTTTTGTAATATACACATATATTGGTGAAATTTCCATATAAATGAGAGATAGTAGATTTTGGAGAGAAATTTTTAATTTAATTTAATTTATTTATATATTATATATTATTTATTTTAATATTATATTTTCTCGATTTTTGTGTTTCTGTCGTGCCTCATTTTTACGACCGTTTGTAAGTTATAAATAAATTATCATACGTGGTCAAAAATTTGGTGACCATAAGTGGATGAATTTAGCATTACAAATGAAATATAATACAAAGGTCCAATATTATGAAAATAGTAACTCCAAAAGTAATTGTGACAACTTAGTTTTATTGAAGAGGGGAATCCCTCAACTAATTATATTTTTTCTACGCGTTTTATAGTCATTCTAATTTTGTTAGAATTTGTAGCACAACAATTATAAAAAGTGGAGTATTGAACGACCTTGGAGACTATAAACTTATTTTTTCACTTAAACATGTGTACAAAAAAAATTTGAAATATAAATATTCTTAATTTTAGAGTTTTTTTACAAAAAGAAAAAGAAAACAAATCCTTATAAAAAAAATAATGATAATAATTAATTTGAATTTTTTTTTAATAAAATTTGTAAGACCCATTAATAAAATAATAATGGGCCGGTCCATGTACTAGGCTTTATATTGTTGGGCCCTTTTCTGAGATGAAGGTCGAGTCCGTTCTTCGCTCTTCTGCTTGAACTTAAAAATGATTTTCTTTTAGGCGGCAACGGATCGTCGGCGAAATTCCGAATTTGTGTTCAATCACCAAGCCGAGGAACAAGTGCGTGTTTTTCTTCTCGGCGGCGAGTTATTTGGCTGCGGTTTACACATTCCAATTCGTTGATCAACATCGTAAGTATCCCATCAAGTTATCATGCCTACGATCTTTAGTGTTATGTATGGAAATTTAGGATGGATTGTTCTTGGATTCGTCTTTTGTTTACCGTCCGCTTCTCGTAATTGGAACGTATTTAGGTTAAGCATCACCGTTTTTAGCTCTTTCAATTATCTATCTAGGTTAGCTTTAAGGTCAAGCAGTGATGAATTGAAGTTTTAGTATGTTGGCTTTTTGGTGAAGGCGATCATACTGCATTTTTGTAATTGATTTTTAGTACACATGTCTCGAAATAGAGTGAAAAGTAGATTGTTCGTAGATGGGAAGTTAGTTCTGACGGTCTTGTTCTGTGTTGTCGAATCTGAATCCATTCTTATAATATATTTGAGAATTTCAGTCTGTTTTTCTTATTTGTTCTTGAGATGATATGTTTCTGGCTAGACTTATTGTTTGGTTTTTGCGGAAGGCAGTATGTAACTGGATCCTGTAATTTCAGATTTGTTGTTTTTTGTATTTTTTGAACAACTATGGCTTTCTGGTACTGGTAGATTGTCATTGTCCATTGTTTAAAAAGATATAACATCCAGCTATTGAAACTGTTTGCATTTTGATATTGGTAAATGCTCTTGTTGTTTTCAGATGAAAACTGCTGATGCATCTTTGTTCAGCATGTTCTTCAAACACAAGGATAAAAGTTCTCCATCCAATGATGATTCAGAGGACCTTGGTGATGTTCTTGATAGTGAGGTACCCGACTAATTTAATTGCATTAGCCTTTGGTTTCTTTTTATTTCTTCTGGTGTTTGTATGGTGTTTTAACCGTAGTTTTTCACAAATTATAGGTGAACTCTTACCTAAACAACAGGAAGGAGGCTCATTATAAAAAGATTATTTGGGAGCAAATTAACAAAGACTATCTTCAGGTTAAATGCTTCTCGCCCCTCTTAATAACCCTTTGATTGCTTCTAAAAAGTCTACACTTATACACATTGGAACAAAGTGTTTTATGTTTCAATCATAGTTTGATTTCTAAATAAATGATATGCTGGTTTTCAGGACCAAGCAGCTAAGCAACAAGGCCTTAATGTCGTTGGAGCTGCAGCTGTGGTTAAAAAATCAAAGAAGGTATTTAAGTAACTACAGTTCGTGTTAGACAAGAAGTCCTTTATCCTGAAAAACATTGGATAGGAATTTTGCATCTTTACATCTTATAATATGATTTATGTTTTCTCGTTCTGTTGGAAAAAAGCAGAGGCAAAGGAAAATAGAAGCTCCAATAAACAAGCCTGCTCAAGCCGATACAGAAACTACACGTGAAATGCTAACTAAAAAGGTATATCAGTGAGATAAACTAATTAATAGATTAATTGGATGGCGGTGTGATCTCTAATTTTACCCCAATGGATCCGTTCTAATCTTAATTTTCATATTACTTTCTAATTCTGTCGGGCCAACTTCCACTCTTAATTATTAATTATTGTCTTATTGGTCCTCTATGGACCACATGTATGTTCTTTTATGAAAATTTTCATTCTTATAATCTCTTTTCAAATCATTTTCTTAAGAAACCCTTGAGTGTAGGATTCATGAACCATCCACATTATTTTTCTGGGGTCATTTTTGTTTTGGTGTGGGGGAAGAGTATTTATTTTCATAATTCCATGGTTTTTGTGCAAGCTAACTTAGTTTGTTTGGTTGCGTTACATCTATCCACTAATTAGCTTATTTCCCACAATCATTCTTCGCAGAGGCTCAGTTCAAAATTCAACTTTGACGTGTTGGACAAACTCTTCTCTGATACAGTATGTTTATCCATCTATTTTCTTAAGAAAAAAAAATCTATCTATTTCTTCTTTAATGTCTTGGTATTTACATGTTCGAATGAGACTGTAATTGTCATCAGTACAGGAAAATAATCACTCCCAGAGAAAGGATGGAAAGAAAGGAATTTAACCTTCTTCATTATTTCGATTACTACACATGGTTAGTATAACTGCCTAATAATTTGTGCAGCCCGCACCTGATTCAAGCGAAAAGCAGGGAGTCTCAAGTTGTAAAGAGAATAGTTCATCTGGTCAAAGCCATTGTAGGAACGAGCTTGAAGATGACTTCGAAGACAATCATACTGATACGATGTTTGAAGAAAATTTTGACATTGAAGGTGAAGATCCCACAGAAACTTATGGTAATGAGTATTATGATTTCGAAAACGAGGAAGAGTATGGCTACGGATACGATGATTATGGTGGTGAGGAGGAATATTAGTGGATCAATTTTTTACACAAAAGCTTCTTAATTTTGGCTGAGGTCATAAGATGGATTCCAGATAACAACAAGAAGTTAACGAGAGAGCTTTAAATTAATTTTGGGTGTTTGGTGACAGATTTAGGAAAACAGCAACAGTCGATGCTTCTCTTTTTTCTTTCCGCTATTGGAAGGAGAGGAGCAATTAGGCAAAGTTCATGCAAGAAGAAAGAAAATCATGAGGTCCAAACTAAAGAGGCTTTCATGAGTCCTGGCAGGGAATCTGAGTTATATGACAAAAGAAAAAAACTTGGGTGGAGCCTCGGTTGTACCCAATGATACACTGCCATGTGACAATTTTTGTCCTTTCAATTTTATATAGTTTTCCTATGTATAAAAAAGGGATGTATTGGGATTAATCATCCCTCATGACAAAAAGGGGAGAAAGAATCATAAACAGGGTTATTTGGATGGGAGAAATAAGACGTAGCTATTTGTCAATTATTGTCTTGTTATCTTTTGATTCAGTCAAATAAAAAATGTGTTAAGA

mRNA sequence

ATGTTGGTGGTGTCACCACGAGTGGGGTTAAATATTGAGATTGTAAGGTATCCGTTCTTCGCTCTTCTGCTTGAACTTAAAAATGATTTTCTTTTAGGCGGCAACGGATCGTCGGCGAAATTCCGAATTTGTGTTCAATCACCAAGCCGAGGAACAAGTGCGTGTTTTTCTTCTCGGCGGCGAGTTATTTGGCTGCGGTTTACACATTCCAATTCGTTGATCAACATCATGAAAACTGCTGATGCATCTTTGTTCAGCATGTTCTTCAAACACAAGGATAAAAGTTCTCCATCCAATGATGATTCAGAGGACCTTGGTGATGTTCTTGATAGTGAGGTGAACTCTTACCTAAACAACAGGAAGGAGGCTCATTATAAAAAGATTATTTGGGAGCAAATTAACAAAGACTATCTTCAGGACCAAGCAGCTAAGCAACAAGGCCTTAATGTCGTTGGAGCTGCAGCTGTGGTTAAAAAATCAAAGAAGAGGCAAAGGAAAATAGAAGCTCCAATAAACAAGCCTGCTCAAGCCGATACAGAAACTACACGTGAAATGCTAACTAAAAAGAGGCTCAGTTCAAAATTCAACTTTGACGTGTTGGACAAACTCTTCTCTGATACACCCGCACCTGATTCAAGCGAAAAGCAGGGAGTCTCAAGTTGTAAAGAGAATAGTTCATCTGGTCAAAGCCATTGTAGGAACGAGCTTGAAGATGACTTCGAAGACAATCATACTGATACGATGTTTGAAGAAAATTTTGACATTGAAGGTGAAGATCCCACAGAAACTTATGGTAATGAGTATTATGATTTCGAAAACGAGGAAGAGTATGGCTACGGATACGATGATTATGGTGGTGAGGAGGAATATTAGTGGATCAATTTTTTACACAAAAGCTTCTTAATTTTGGCTGAGGTCATAAGATGGATTCCAGATAACAACAAGAAGTTAACGAGAGAGCTTTAAATTAATTTTGGGTGTTTGGTGACAGATTTAGGAAAACAGCAACAGTCGATGCTTCTCTTTTTTCTTTCCGCTATTGGAAGGAGAGGAGCAATTAGGCAAAGTTCATGCAAGAAGAAAGAAAATCATGAGGTCCAAACTAAAGAGGCTTTCATGAGTCCTGGCAGGGAATCTGAGTTATATGACAAAAGAAAAAAACTTGGGTGGAGCCTCGGTTGTACCCAATGATACACTGCCATGTGACAATTTTTGTCCTTTCAATTTTATATAGTTTTCCTATGTATAAAAAAGGGATGTATTGGGATTAATCATCCCTCATGACAAAAAGGGGAGAAAGAATCATAAACAGGGTTATTTGGATGGGAGAAATAAGACGTAGCTATTTGTCAATTATTGTCTTGTTATCTTTTGATTCAGTCAAATAAAAAATGTGTTAAGA

Coding sequence (CDS)

ATGTTGGTGGTGTCACCACGAGTGGGGTTAAATATTGAGATTGTAAGGTATCCGTTCTTCGCTCTTCTGCTTGAACTTAAAAATGATTTTCTTTTAGGCGGCAACGGATCGTCGGCGAAATTCCGAATTTGTGTTCAATCACCAAGCCGAGGAACAAGTGCGTGTTTTTCTTCTCGGCGGCGAGTTATTTGGCTGCGGTTTACACATTCCAATTCGTTGATCAACATCATGAAAACTGCTGATGCATCTTTGTTCAGCATGTTCTTCAAACACAAGGATAAAAGTTCTCCATCCAATGATGATTCAGAGGACCTTGGTGATGTTCTTGATAGTGAGGTGAACTCTTACCTAAACAACAGGAAGGAGGCTCATTATAAAAAGATTATTTGGGAGCAAATTAACAAAGACTATCTTCAGGACCAAGCAGCTAAGCAACAAGGCCTTAATGTCGTTGGAGCTGCAGCTGTGGTTAAAAAATCAAAGAAGAGGCAAAGGAAAATAGAAGCTCCAATAAACAAGCCTGCTCAAGCCGATACAGAAACTACACGTGAAATGCTAACTAAAAAGAGGCTCAGTTCAAAATTCAACTTTGACGTGTTGGACAAACTCTTCTCTGATACACCCGCACCTGATTCAAGCGAAAAGCAGGGAGTCTCAAGTTGTAAAGAGAATAGTTCATCTGGTCAAAGCCATTGTAGGAACGAGCTTGAAGATGACTTCGAAGACAATCATACTGATACGATGTTTGAAGAAAATTTTGACATTGAAGGTGAAGATCCCACAGAAACTTATGGTAATGAGTATTATGATTTCGAAAACGAGGAAGAGTATGGCTACGGATACGATGATTATGGTGGTGAGGAGGAATATTAG

Protein sequence

MLVVSPRVGLNIEIVRYPFFALLLELKNDFLLGGNGSSAKFRICVQSPSRGTSACFSSRRRVIWLRFTHSNSLINIMKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKQQGLNVVGAAAVVKKSKKRQRKIEAPINKPAQADTETTREMLTKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDDFEDNHTDTMFEENFDIEGEDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY

Homology

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BLAST of PI0008307 vs. ExPASy Swiss-Prot
Match: Q9P6R0 (Transcription factor IIIB 60 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=brf1 PE=1 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 7.8e-06
Identity = 34/110 (30.91%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 99  NDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKQQGLNVVGAAAVVK 158
           +++   L DV D E+   L ++ E   K  +W ++NKDYL ++ AK   L       +V+
Sbjct: 380 SEEEVTLEDVDDDEIEDILLDKDEILTKTQVWMELNKDYLAEEEAKNLKLQEDLKKGIVR 439

Query: 159 KSKKRQRKIEAPINKPAQADT--ETTREMLTKKRLSSKFNFDVLDKLFSD 207
           + +KR+R           ADT  E+ +EM+ ++  S K N++ LD LF +
Sbjct: 440 QPRKRRRYRPRDSTSDGIADTAAESAKEMMQQRAFSKKINYEALDMLFDE 489

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BLAST of PI0008307 vs. ExPASy Swiss-Prot
Match: Q92994 (Transcription factor IIIB 90 kDa subunit OS=Homo sapiens OX=9606 GN=BRF1 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 8.6e-05
Identity = 37/114 (32.46%), Postives = 56/114 (49.12%), Query Frame = 0


Query: 93  DKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKQQGLNVVG 152
           D    S D   DL  + D E++ Y+ N  EA  K  +W + N +YL++Q  K+  +    
Sbjct: 437 DPKDASGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKEARIAKEK 496

Query: 153 AAAVVKKSK-KRQRKIEAPINKPAQADTETTREMLTKKRLSSKFNFDVLDKLFS 206
              + K+ K K+  K   PI   A    E   +ML +K++SSK N+ VL  L S
Sbjct: 497 ELGIYKEHKPKKSCKRREPIQ--ASTAREAIEKMLEQKKISSKINYSVLRGLSS 548

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BLAST of PI0008307 vs. ExPASy Swiss-Prot
Match: Q8CFK2 (Transcription factor IIIB 90 kDa subunit OS=Mus musculus OX=10090 GN=Brf1 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 45/149 (30.20%), Postives = 71/149 (47.65%), Query Frame = 0


Query: 93  DKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKQQGLNVVG 152
           D    S D   DL  + D E++ Y+ N  EA  K  +W + N +YL++Q  K+  +    
Sbjct: 436 DPKDSSGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQKEKEARIAKEK 495

Query: 153 AAAVVKKSK-KRQRKIEAPINKPAQADTETTREMLTKKRLSSKFNFDVLDKLFS---DTP 212
              + K+ K K+  K   PI   A    E   +ML +K++SSK N+ VL  L S    +P
Sbjct: 496 ELGIYKEHKPKKSCKRREPI--LASTAGEAIEKMLEQKKISSKINYSVLRDLNSKGGGSP 555

Query: 213 APDSSEKQGVSSCKENSSSGQSHCRNELE 238
             D S+    +S K+ S   ++  RN  +
Sbjct: 556 PRDDSQPPERASTKKLSRRKRATTRNSAD 582

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BLAST of PI0008307 vs. ExPASy TrEMBL
Match: A0A0A0L0J7 (BRF1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G363410 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 6.8e-114
Identity = 237/312 (75.96%), Postives = 254/312 (81.41%), Query Frame = 0


Query: 1   MLVVSPRVGLNIEIVRYPFFALLLELKNDFLLGGNGSSAKFRICVQSPSRGTSACFSSRR 60
           MLVVSP   L ++I+RY         +   ++G      K   CV+S + GTSACFSSRR
Sbjct: 1   MLVVSP---LELKILRY-------IARRQRIVG------KIPDCVKSLTGGTSACFSSRR 60

Query: 61  RVIWLRFTHSNSLINIMKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNR 120
           RVIWLR+THS+SLINIMKTADASLF MFFKHKDKSSPS+DDSEDLGDV DSEVNSYLNNR
Sbjct: 61  RVIWLRYTHSDSLINIMKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNR 120

Query: 121 KEAHYKKIIWEQINKDYLQDQAAKQQGLNVVGAAAVVKKSKKRQRKIEAPINKPAQADTE 180
           KEAHYKKIIWEQINKDYLQDQAAK+QGLNVVGA+AVVKKSKKRQRK EAPIN PAQADT 
Sbjct: 121 KEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQADTG 180

Query: 181 TTREMLTKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDD- 240
           TTREM  KKRLSSKFNFDVLDKLFSDTPAPDSSEKQG SSCKENSSSGQ HCRNELEDD 
Sbjct: 181 TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDF 240

Query: 241 --------------------FEDNHTDTMFEENFDIEG-EDPTETYGNEYYDFENEEEYG 291
                               FEDNHT+TMFEENFDIEG EDPTETYGNEYYDF++EEEYG
Sbjct: 241 EDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDIEGEEDPTETYGNEYYDFQDEEEYG 296

[Close]

BLAST of PI0008307 vs. ExPASy TrEMBL
Match: A0A5D3E7S6 (Transcription factor IIIB 90 kDa subunit-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold398G00100 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.6e-99
Identity = 196/215 (91.16%), Postives = 204/215 (94.88%), Query Frame = 0


Query: 77  MKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKD 136
           MK ADASLFSMF +HKDKSSPSNDDSEDLGDV DSEVNSYLNNRKEAHYKKIIWEQINKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 137 YLQDQAAKQQGLNVVGAAAVVKKSKKRQRKIEAPINKPAQADT-ETTREMLTKKRLSSKF 196
           YLQDQAAK+QGLN+VGAAAVVKKSKKR+RKIEAPINKPAQADT ETT EML KKRLSSKF
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKRKRKIEAPINKPAQADTEETTHEMLIKKRLSSKF 120

Query: 197 NFDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDDFEDNHTDTMFEENFDI 256
           +FDVLDKL  DTPAPDSSEKQG SSCKENSSSGQSHC NELEDDFEDNHT+T+FEEN DI
Sbjct: 121 DFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLDI 180

Query: 257 EGEDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
           EGEDPTETYGNEYYDF+NEEEYGYGYDDYGGEEEY
Sbjct: 181 EGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 215

[Close]

BLAST of PI0008307 vs. ExPASy TrEMBL
Match: A0A1S3B993 (uncharacterized protein LOC103487591 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487591 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.6e-99
Identity = 196/215 (91.16%), Postives = 204/215 (94.88%), Query Frame = 0


Query: 77  MKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKD 136
           MK ADASLFSMF +HKDKSSPSNDDSEDLGDV DSEVNSYLNNRKEAHYKKIIWEQINKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 137 YLQDQAAKQQGLNVVGAAAVVKKSKKRQRKIEAPINKPAQADT-ETTREMLTKKRLSSKF 196
           YLQDQAAK+QGLN+VGAAAVVKKSKKR+RKIEAPINKPAQADT ETT EML KKRLSSKF
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKRKRKIEAPINKPAQADTEETTHEMLIKKRLSSKF 120

Query: 197 NFDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDDFEDNHTDTMFEENFDI 256
           +FDVLDKL  DTPAPDSSEKQG SSCKENSSSGQSHC NELEDDFEDNHT+T+FEEN DI
Sbjct: 121 DFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLDI 180

Query: 257 EGEDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
           EGEDPTETYGNEYYDF+NEEEYGYGYDDYGGEEEY
Sbjct: 181 EGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 215

[Close]

BLAST of PI0008307 vs. ExPASy TrEMBL
Match: A0A1S4DV37 (uncharacterized protein LOC103487591 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487591 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 4.0e-98
Identity = 196/216 (90.74%), Postives = 204/216 (94.44%), Query Frame = 0


Query: 77  MKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKD 136
           MK ADASLFSMF +HKDKSSPSNDDSEDLGDV DSEVNSYLNNRKEAHYKKIIWEQINKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 137 YLQDQAAKQQGLNVVGAAAVVKKSKK-RQRKIEAPINKPAQADT-ETTREMLTKKRLSSK 196
           YLQDQAAK+QGLN+VGAAAVVKKSKK R+RKIEAPINKPAQADT ETT EML KKRLSSK
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKQRKRKIEAPINKPAQADTEETTHEMLIKKRLSSK 120

Query: 197 FNFDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDDFEDNHTDTMFEENFD 256
           F+FDVLDKL  DTPAPDSSEKQG SSCKENSSSGQSHC NELEDDFEDNHT+T+FEEN D
Sbjct: 121 FDFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLD 180

Query: 257 IEGEDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
           IEGEDPTETYGNEYYDF+NEEEYGYGYDDYGGEEEY
Sbjct: 181 IEGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 216

[Close]

BLAST of PI0008307 vs. ExPASy TrEMBL
Match: A0A1S3B9Q2 (uncharacterized protein LOC103487591 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103487591 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.6e-83
Identity = 172/214 (80.37%), Postives = 179/214 (83.64%), Query Frame = 0


Query: 77  MKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKD 136
           MK ADASLFSMF +HKDKSSPSNDDSEDLGDV DSEVNSYLNNRKEAHYKKIIWEQINKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 137 YLQDQAAKQQGLNVVGAAAVVKKSKKRQRKIEAPINKPAQADTETTREMLTKKRLSSKFN 196
           YLQDQAAK+QGLN+VGAAAVVKKS                           KKRLSSKF+
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKS---------------------------KKRLSSKFD 120

Query: 197 FDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDDFEDNHTDTMFEENFDIE 256
           FDVLDKL  DTPAPDSSEKQG SSCKENSSSGQSHC NELEDDFEDNHT+T+FEEN DIE
Sbjct: 121 FDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLDIE 180

Query: 257 GEDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
           GEDPTETYGNEYYDF+NEEEYGYGYDDYGGEEEY
Sbjct: 181 GEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 187

[Close]

BLAST of PI0008307 vs. NCBI nr
Match: KAE8649624.1 (hypothetical protein Csa_012863 [Cucumis sativus])

HSP 1 Score: 417.9 bits (1073), Expect = 7.0e-113
Identity = 225/269 (83.64%), Postives = 236/269 (87.73%), Query Frame = 0


Query: 44  CVQSPSRGTSACFSSRRRVIWLRFTHSNSLINIMKTADASLFSMFFKHKDKSSPSNDDSE 103
           CV+S + GTSACFSSRRRVIWLR+THS+SLINIMKTADASLF MFFKHKDKSSPS+DDSE
Sbjct: 107 CVKSLTGGTSACFSSRRRVIWLRYTHSDSLINIMKTADASLFGMFFKHKDKSSPSDDDSE 166

Query: 104 DLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKQQGLNVVGAAAVVKKSKKR 163
           DLGDV DSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAK+QGLNVVGA+AVVKKSKKR
Sbjct: 167 DLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKR 226

Query: 164 QRKIEAPINKPAQADTETTREMLTKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGVSSCKE 223
           QRK EAPIN PAQADT TTREM  KKRLSSKFNFDVLDKLFSDTPAPDSSEKQG SSCKE
Sbjct: 227 QRKTEAPINMPAQADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKE 286

Query: 224 NSSSGQSHCRNELEDD---------------------FEDNHTDTMFEENFDIEG-EDPT 283
           NSSSGQ HCRNELEDD                     FEDNHT+TMFEENFDIEG EDPT
Sbjct: 287 NSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDIEGEEDPT 346

Query: 284 ETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
           ETYGNEYYDF++EEEYGYGY+DYGGEEEY
Sbjct: 347 ETYGNEYYDFQDEEEYGYGYNDYGGEEEY 375

[Close]

BLAST of PI0008307 vs. NCBI nr
Match: XP_008444162.1 (PREDICTED: uncharacterized protein LOC103487591 isoform X2 [Cucumis melo] >TYK31660.1 transcription factor IIIB 90 kDa subunit-like isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 372.5 bits (955), Expect = 3.4e-99
Identity = 196/215 (91.16%), Postives = 204/215 (94.88%), Query Frame = 0


Query: 77  MKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKD 136
           MK ADASLFSMF +HKDKSSPSNDDSEDLGDV DSEVNSYLNNRKEAHYKKIIWEQINKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 137 YLQDQAAKQQGLNVVGAAAVVKKSKKRQRKIEAPINKPAQADT-ETTREMLTKKRLSSKF 196
           YLQDQAAK+QGLN+VGAAAVVKKSKKR+RKIEAPINKPAQADT ETT EML KKRLSSKF
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKRKRKIEAPINKPAQADTEETTHEMLIKKRLSSKF 120

Query: 197 NFDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDDFEDNHTDTMFEENFDI 256
           +FDVLDKL  DTPAPDSSEKQG SSCKENSSSGQSHC NELEDDFEDNHT+T+FEEN DI
Sbjct: 121 DFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLDI 180

Query: 257 EGEDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
           EGEDPTETYGNEYYDF+NEEEYGYGYDDYGGEEEY
Sbjct: 181 EGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 215

[Close]

BLAST of PI0008307 vs. NCBI nr
Match: XP_016899849.1 (PREDICTED: uncharacterized protein LOC103487591 isoform X1 [Cucumis melo])

HSP 1 Score: 367.9 bits (943), Expect = 8.3e-98
Identity = 196/216 (90.74%), Postives = 204/216 (94.44%), Query Frame = 0


Query: 77  MKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKD 136
           MK ADASLFSMF +HKDKSSPSNDDSEDLGDV DSEVNSYLNNRKEAHYKKIIWEQINKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 137 YLQDQAAKQQGLNVVGAAAVVKKSKK-RQRKIEAPINKPAQADT-ETTREMLTKKRLSSK 196
           YLQDQAAK+QGLN+VGAAAVVKKSKK R+RKIEAPINKPAQADT ETT EML KKRLSSK
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKQRKRKIEAPINKPAQADTEETTHEMLIKKRLSSK 120

Query: 197 FNFDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDDFEDNHTDTMFEENFD 256
           F+FDVLDKL  DTPAPDSSEKQG SSCKENSSSGQSHC NELEDDFEDNHT+T+FEEN D
Sbjct: 121 FDFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLD 180

Query: 257 IEGEDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
           IEGEDPTETYGNEYYDF+NEEEYGYGYDDYGGEEEY
Sbjct: 181 IEGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 216

[Close]

BLAST of PI0008307 vs. NCBI nr
Match: XP_004142699.1 (uncharacterized protein LOC101217202 isoform X2 [Cucumis sativus])

HSP 1 Score: 364.0 bits (933), Expect = 1.2e-96
Identity = 198/236 (83.90%), Postives = 205/236 (86.86%), Query Frame = 0


Query: 77  MKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKD 136
           MKTADASLF MFFKHKDKSSPS+DDSEDLGDV DSEVNSYLNNRKEAHYKKIIWEQINKD
Sbjct: 1   MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 137 YLQDQAAKQQGLNVVGAAAVVKKSKKRQRKIEAPINKPAQADTETTREMLTKKRLSSKFN 196
           YLQDQAAK+QGLNVVGA+AVVKKSKKRQRK EAPIN PAQADT TTREM  KKRLSSKFN
Sbjct: 61  YLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQADTGTTREMQIKKRLSSKFN 120

Query: 197 FDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDD----------------- 256
           FDVLDKLFSDTPAPDSSEKQG SSCKENSSSGQ HCRNELEDD                 
Sbjct: 121 FDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNE 180

Query: 257 ----FEDNHTDTMFEENFDIEG-EDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
               FEDNHT+TMFEENFDIEG EDPTETYGNEYYDF++EEEYGYGY+DYGGEEEY
Sbjct: 181 LEDNFEDNHTETMFEENFDIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY 236

[Close]

BLAST of PI0008307 vs. NCBI nr
Match: XP_011653794.1 (uncharacterized protein LOC101217202 isoform X1 [Cucumis sativus])

HSP 1 Score: 359.4 bits (921), Expect = 3.0e-95
Identity = 198/237 (83.54%), Postives = 205/237 (86.50%), Query Frame = 0


Query: 77  MKTADASLFSMFFKHKDKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKD 136
           MKTADASLF MFFKHKDKSSPS+DDSEDLGDV DSEVNSYLNNRKEAHYKKIIWEQINKD
Sbjct: 1   MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 137 YLQDQAAKQQGLNVVGAAAVVKKSKK-RQRKIEAPINKPAQADTETTREMLTKKRLSSKF 196
           YLQDQAAK+QGLNVVGA+AVVKKSKK RQRK EAPIN PAQADT TTREM  KKRLSSKF
Sbjct: 61  YLQDQAAKKQGLNVVGASAVVKKSKKQRQRKTEAPINMPAQADTGTTREMQIKKRLSSKF 120

Query: 197 NFDVLDKLFSDTPAPDSSEKQGVSSCKENSSSGQSHCRNELEDD---------------- 256
           NFDVLDKLFSDTPAPDSSEKQG SSCKENSSSGQ HCRNELEDD                
Sbjct: 121 NFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRN 180

Query: 257 -----FEDNHTDTMFEENFDIEG-EDPTETYGNEYYDFENEEEYGYGYDDYGGEEEY 291
                FEDNHT+TMFEENFDIEG EDPTETYGNEYYDF++EEEYGYGY+DYGGEEEY
Sbjct: 181 ELEDNFEDNHTETMFEENFDIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY 237

[Close]

BLAST of PI0008307 vs. TAIR 10
Match: AT3G09360.1 (Cyclin/Brf1-like TBP-binding protein )

HSP 1 Score: 82.8 bits (203), Expect = 5.0e-16
Identity = 68/217 (31.34%), Postives = 105/217 (48.39%), Query Frame = 0


Query: 93  DKSSPSNDDSEDLGDVLDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKQQGLNVVG 152
           ++ + ++D+S++  D+ D EVN Y+NN +E HYK I W ++NKDYL++QAAK+  L    
Sbjct: 387 EEHADTSDESDNFSDISDDEVNGYINNEEETHYKTITWTEMNKDYLEEQAAKEAALKAAS 446

Query: 153 AA----------------------AVVKKSKKRQRKIEAPIN-KPAQADTETTREMLTKK 212
            A                      A   + +K+Q+K E   N  P     E  R  L KK
Sbjct: 447 EALKASNSNCPEDARKAFEAAKADAAKSRKEKQQKKAEEAKNAAPPATAVEAVRRTLDKK 506

Query: 213 RLSSKFNFDVLDKLFSDTPAPDSSEKQGVSSCK-ENSSSGQSHCRNELEDDFEDNHTDTM 272
           RLSS  N+DVL+ LF DT AP+ S K+  +    E         ++   ++ E+   D  
Sbjct: 507 RLSSVINYDVLESLF-DTSAPEKSPKRSKTETDIEKKKEENKEMKSNEHENGENEDEDEE 566

Query: 273 FEENFDIEGEDPTETYGNEYYDFENEEEYGYGYDDYG 286
            EE  ++E  D    + N    +E +EE     +D+G
Sbjct: 567 DEEEGNVESYDMKTDFQNGEKFYEEDEEEEEDGNDFG 602

The following BLAST results are available for this feature:

BLAST of PI0008307 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9P6R0	7.8e-06	30.91	Transcription factor IIIB 60 kDa subunit OS=Schizosaccharomyces pombe (strain 97...	[more]
Q92994	8.6e-05	32.46	Transcription factor IIIB 90 kDa subunit OS=Homo sapiens OX=9606 GN=BRF1 PE=1 SV...	[more]
Q8CFK2	1.9e-04	30.20	Transcription factor IIIB 90 kDa subunit OS=Mus musculus OX=10090 GN=Brf1 PE=1 S...	[more]

BLAST of PI0008307 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0J7	6.8e-114	75.96	BRF1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G363410 PE=4 S...	[more]
A0A5D3E7S6	1.6e-99	91.16	Transcription factor IIIB 90 kDa subunit-like isoform X3 OS=Cucumis melo var. ma...	[more]
A0A1S3B993	1.6e-99	91.16	uncharacterized protein LOC103487591 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S4DV37	4.0e-98	90.74	uncharacterized protein LOC103487591 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S3B9Q2	1.6e-83	80.37	uncharacterized protein LOC103487591 isoform X3 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of PI0008307 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649624.1	7.0e-113	83.64	hypothetical protein Csa_012863 [Cucumis sativus]	[more]
XP_008444162.1	3.4e-99	91.16	PREDICTED: uncharacterized protein LOC103487591 isoform X2 [Cucumis melo] >TYK31...	[more]
XP_016899849.1	8.3e-98	90.74	PREDICTED: uncharacterized protein LOC103487591 isoform X1 [Cucumis melo]	[more]
XP_004142699.1	1.2e-96	83.90	uncharacterized protein LOC101217202 isoform X2 [Cucumis sativus]	[more]
XP_011653794.1	3.0e-95	83.54	uncharacterized protein LOC101217202 isoform X1 [Cucumis sativus]	[more]

BLAST of PI0008307 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09360.1	5.0e-16	31.34	Cyclin/Brf1-like TBP-binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011665	Brf1, TBP-binding domain	PFAM	PF07741	BRF1	coord: 107..205 e-value: 8.1E-22 score: 77.4
None	No IPR available	GENE3D	1.20.5.650	Single helix bin	coord: 84..147 e-value: 9.2E-17 score: 62.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 232..251
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 209..231
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 162..183
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 207..290
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 252..290
None	No IPR available	PANTHER	PTHR11618:SF53	PLANT-SPECIFIC TFIIB-RELATED PROTEIN 2	coord: 59..270
IPR000812	Transcription factor TFIIB	PANTHER	PTHR11618	TRANSCRIPTION INITIATION FACTOR IIB-RELATED	coord: 59..270

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0008307.1	PI0008307.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription by RNA polymerase III
biological_process	GO:0070897	transcription preinitiation complex assembly
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0016020	membrane
cellular_component	GO:0000126	transcription factor TFIIIB complex
molecular_function	GO:0000995	RNA polymerase III general transcription initiation factor activity