PI0007721 (gene) Melon (PI 482460) v1

Overview
NamePI0007721
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectinesterase
Locationchr11: 4071625 .. 4073641 (+)
RNA-Seq ExpressionPI0007721
SyntenyPI0007721
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAAAAAAAACCCCAAAAAAATCATTTTTTTTTCATTCTTTCAAAATGTCAAACGGATTTGCCAATGCCGAAGGTGATAAAAAGAAGAAGAAGATTGCCATCATTAGTGTGTCTTCTTTAATCCTCGTAGCAATGGTTGTCGCCGTTACCGTCGGCGTCAACAGTGCACCCCACGGAGAGCACAGTGACGATGATCATGGCAGCGAAGCCACCCCTGAAATCTCGTCATCGACCAAAGCTATCCAAGCCATTTGTCAACCAACGGATTACAAAGAAACCTGTGAGAAGAGTCTAATGGCAGAAGCCGGTAACACAACAGACCCAAAAGAGCTAGTGAAAGTTGGCTTCAAAATCGCCACAAGGAGTCTAAACGAGGCCATTAGGAATTCGACCACACTTCAAGAATTAGCCAAAGACCCCAGAACCAACCAAGCCCTTCAAAATTGTCGTGAACTACTAGAATATGCCATTGATGATTTGAACGAGTCATTCGAAAGAATCGGCACCTTCCAAATGTCCAAACTCGACGAATTCGTCGCCGATTTAAAGATTTGGCTCAGTGGTGCACTCACGTATGAACAAACTTGCTTGGATGGTTTTGAAAACACTACAGGTGATGCTGGTGCCAGAATGCAAGAGTTTTTGAAGTCAGCTCAGCAAATGACAACCAATGGCCTTGGCATTGTTAATGAATTGTCTTCCATTTTGGGTTCACTTCAGATTCCTGGAATGACTGGACGACGACTTTTGGAAGATGATGATCATGAAATGCCATCATGGGTTAGCGACGGAAAGAGACGATTGATGCAGGCTGGAGCCGCGTCGATGAAACCCGATTTGGTTGTAGCTCAAGATGGGAGTGGGAAATACAAAACCATAAATGCAGCTTTAGTTGATGTGCCTTTGAAGAGTAATAAAACGTTTGTGATTTATGTGAAGGCTGGAATTTACAAAGAAACTGTTGTCATCCCCAAACATATGACTCATCTTACAATGTACGGTGATGGTCCAACCAAAACTGTCGTCACTGGTAGCTTAAACTTCATCGATGGTATTCAAACATTCAAGACCGCTACCTTCTGTAAGTATAGTATCATTTGATTTGATTTGGTTTGATTTGATTAGTTGTCTAATTAATTGATATGATTCAATTTGATTCAGCTGCAATTGGAGCTAACTTCTACGCTAGAGATATGGGATTCGAAAACACAGCTGGATCAGCAAAGCACCAAGCCGTGGCCCTACGAGTCCAATCCGACAGATCAATCTTCTACAACTGCCAAATCGACGGATACCAAGATACACTCTACGCGCACGCTCATCGTCAGTTCTACAGAGATTGCACAATCAGTGGCACCATCGACTTCGTCTTCGGAAACGCTGCTACAAATTTCCAGAACTGCAAGCTAGTGGTGCGAAAGCCATTGGATAACCAACAATGCATCGTAACGGCTCACGGGCGTCTGAACCGCAAGGAACCAACGGCATTGATCTTCCAGAGCTGCCATTTCATGGGTGATCCAGGTTACTTGCCATTCAAGAGCATCAACAAGGCTTATTTGGGACGTCCATGGAAGGAATATTCAAGAACCATTATAATCGGATCTCAAATAGACGATATAATCCAGCCAGAAGGATGGTTGCCATGGATGGGAAATTTTGGATTGAACACCTTGTTCTACGCGGAGGTACAGAACAAAGGGCCAGGGGCTGATGAAAGTAAGAGAGTGAAGTGGAGAGGAATTAAACATATTACGCCACAACATGCTGCGGACTTCACTCCAAGACGATTTATTGATGGCGATGCTTGGATTCCTGCTAAGGGAATTCCTTACTCTTCTGGAATGATGTAATGAACAAAGGGTTTTAAGAAACAGAACCTAAACCCTTTTTTTTTCTCTTAATTTTCTCTTTCTTTTTCTTACACACTTTTGGCGTTTGTTTAATGGAAATCCTATGGAACAAATAAATTTACTTATATTATTTATACTTCTATTTTCTGAA

mRNA sequence

AAAAAAAAAAAAAAAAAAAAAAACCCCAAAAAAATCATTTTTTTTTCATTCTTTCAAAATGTCAAACGGATTTGCCAATGCCGAAGGTGATAAAAAGAAGAAGAAGATTGCCATCATTAGTGTGTCTTCTTTAATCCTCGTAGCAATGGTTGTCGCCGTTACCGTCGGCGTCAACAGTGCACCCCACGGAGAGCACAGTGACGATGATCATGGCAGCGAAGCCACCCCTGAAATCTCGTCATCGACCAAAGCTATCCAAGCCATTTGTCAACCAACGGATTACAAAGAAACCTGTGAGAAGAGTCTAATGGCAGAAGCCGGTAACACAACAGACCCAAAAGAGCTAGTGAAAGTTGGCTTCAAAATCGCCACAAGGAGTCTAAACGAGGCCATTAGGAATTCGACCACACTTCAAGAATTAGCCAAAGACCCCAGAACCAACCAAGCCCTTCAAAATTGTCGTGAACTACTAGAATATGCCATTGATGATTTGAACGAGTCATTCGAAAGAATCGGCACCTTCCAAATGTCCAAACTCGACGAATTCGTCGCCGATTTAAAGATTTGGCTCAGTGGTGCACTCACGTATGAACAAACTTGCTTGGATGGTTTTGAAAACACTACAGGTGATGCTGGTGCCAGAATGCAAGAGTTTTTGAAGTCAGCTCAGCAAATGACAACCAATGGCCTTGGCATTGTTAATGAATTGTCTTCCATTTTGGGTTCACTTCAGATTCCTGGAATGACTGGACGACGACTTTTGGAAGATGATGATCATGAAATGCCATCATGGGTTAGCGACGGAAAGAGACGATTGATGCAGGCTGGAGCCGCGTCGATGAAACCCGATTTGGTTGTAGCTCAAGATGGGAGTGGGAAATACAAAACCATAAATGCAGCTTTAGTTGATGTGCCTTTGAAGAGTAATAAAACGTTTGTGATTTATGTGAAGGCTGGAATTTACAAAGAAACTGTTGTCATCCCCAAACATATGACTCATCTTACAATGTACGGTGATGGTCCAACCAAAACTGTCGTCACTGGTAGCTTAAACTTCATCGATGGTATTCAAACATTCAAGACCGCTACCTTCTCTGCAATTGGAGCTAACTTCTACGCTAGAGATATGGGATTCGAAAACACAGCTGGATCAGCAAAGCACCAAGCCGTGGCCCTACGAGTCCAATCCGACAGATCAATCTTCTACAACTGCCAAATCGACGGATACCAAGATACACTCTACGCGCACGCTCATCGTCAGTTCTACAGAGATTGCACAATCAGTGGCACCATCGACTTCGTCTTCGGAAACGCTGCTACAAATTTCCAGAACTGCAAGCTAGTGGTGCGAAAGCCATTGGATAACCAACAATGCATCGTAACGGCTCACGGGCGTCTGAACCGCAAGGAACCAACGGCATTGATCTTCCAGAGCTGCCATTTCATGGGTGATCCAGGTTACTTGCCATTCAAGAGCATCAACAAGGCTTATTTGGGACGTCCATGGAAGGAATATTCAAGAACCATTATAATCGGATCTCAAATAGACGATATAATCCAGCCAGAAGGATGGTTGCCATGGATGGGAAATTTTGGATTGAACACCTTGTTCTACGCGGAGGTACAGAACAAAGGGCCAGGGGCTGATGAAAGTAAGAGAGTGAAGTGGAGAGGAATTAAACATATTACGCCACAACATGCTGCGGACTTCACTCCAAGACGATTTATTGATGGCGATGCTTGGATTCCTGCTAAGGGAATTCCTTACTCTTCTGGAATGATGTAATGAACAAAGGGTTTTAAGAAACAGAACCTAAACCCTTTTTTTTTCTCTTAATTTTCTCTTTCTTTTTCTTACACACTTTTGGCGTTTGTTTAATGGAAATCCTATGGAACAAATAAATTTACTTATATTATTTATACTTCTATTTTCTGAA

Coding sequence (CDS)

ATGTCAAACGGATTTGCCAATGCCGAAGGTGATAAAAAGAAGAAGAAGATTGCCATCATTAGTGTGTCTTCTTTAATCCTCGTAGCAATGGTTGTCGCCGTTACCGTCGGCGTCAACAGTGCACCCCACGGAGAGCACAGTGACGATGATCATGGCAGCGAAGCCACCCCTGAAATCTCGTCATCGACCAAAGCTATCCAAGCCATTTGTCAACCAACGGATTACAAAGAAACCTGTGAGAAGAGTCTAATGGCAGAAGCCGGTAACACAACAGACCCAAAAGAGCTAGTGAAAGTTGGCTTCAAAATCGCCACAAGGAGTCTAAACGAGGCCATTAGGAATTCGACCACACTTCAAGAATTAGCCAAAGACCCCAGAACCAACCAAGCCCTTCAAAATTGTCGTGAACTACTAGAATATGCCATTGATGATTTGAACGAGTCATTCGAAAGAATCGGCACCTTCCAAATGTCCAAACTCGACGAATTCGTCGCCGATTTAAAGATTTGGCTCAGTGGTGCACTCACGTATGAACAAACTTGCTTGGATGGTTTTGAAAACACTACAGGTGATGCTGGTGCCAGAATGCAAGAGTTTTTGAAGTCAGCTCAGCAAATGACAACCAATGGCCTTGGCATTGTTAATGAATTGTCTTCCATTTTGGGTTCACTTCAGATTCCTGGAATGACTGGACGACGACTTTTGGAAGATGATGATCATGAAATGCCATCATGGGTTAGCGACGGAAAGAGACGATTGATGCAGGCTGGAGCCGCGTCGATGAAACCCGATTTGGTTGTAGCTCAAGATGGGAGTGGGAAATACAAAACCATAAATGCAGCTTTAGTTGATGTGCCTTTGAAGAGTAATAAAACGTTTGTGATTTATGTGAAGGCTGGAATTTACAAAGAAACTGTTGTCATCCCCAAACATATGACTCATCTTACAATGTACGGTGATGGTCCAACCAAAACTGTCGTCACTGGTAGCTTAAACTTCATCGATGGTATTCAAACATTCAAGACCGCTACCTTCTCTGCAATTGGAGCTAACTTCTACGCTAGAGATATGGGATTCGAAAACACAGCTGGATCAGCAAAGCACCAAGCCGTGGCCCTACGAGTCCAATCCGACAGATCAATCTTCTACAACTGCCAAATCGACGGATACCAAGATACACTCTACGCGCACGCTCATCGTCAGTTCTACAGAGATTGCACAATCAGTGGCACCATCGACTTCGTCTTCGGAAACGCTGCTACAAATTTCCAGAACTGCAAGCTAGTGGTGCGAAAGCCATTGGATAACCAACAATGCATCGTAACGGCTCACGGGCGTCTGAACCGCAAGGAACCAACGGCATTGATCTTCCAGAGCTGCCATTTCATGGGTGATCCAGGTTACTTGCCATTCAAGAGCATCAACAAGGCTTATTTGGGACGTCCATGGAAGGAATATTCAAGAACCATTATAATCGGATCTCAAATAGACGATATAATCCAGCCAGAAGGATGGTTGCCATGGATGGGAAATTTTGGATTGAACACCTTGTTCTACGCGGAGGTACAGAACAAAGGGCCAGGGGCTGATGAAAGTAAGAGAGTGAAGTGGAGAGGAATTAAACATATTACGCCACAACATGCTGCGGACTTCACTCCAAGACGATTTATTGATGGCGATGCTTGGATTCCTGCTAAGGGAATTCCTTACTCTTCTGGAATGATGTAA

Protein sequence

MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEISSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDDHEMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYLGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Homology
BLAST of PI0007721 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 659.1 bits (1699), Expect = 4.7e-188
Identity = 308/577 (53.38%), Postives = 426/577 (73.83%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEIS 60
           MS G+   +  K+K++  +I++SS++L++MVVAVTVGV+   H      D  S+   E++
Sbjct: 1   MSYGY--DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKH------DGDSKGKAEVN 60

Query: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120
           +S KA++ +C PTDY++TCE +L+    NTTDP ELVK  F +  + + +A + S T+ E
Sbjct: 61  ASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIME 120

Query: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180
           L KD RT  AL  C+EL++YA+D+L+ SFE +G F+   LDE + +L+IWLS A+++E+T
Sbjct: 121 LQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEET 180

Query: 181 CLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDDH 240
           CL+GF+ T G+AG  M++ LK+A ++T NGL I++E+S+ +G +QIPG+  RRLL +   
Sbjct: 181 CLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEG-- 240

Query: 241 EMPSWVSDGKRRLMQAGAA--SMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVK 300
             PSWV    R+L+QA AA   +KPD+VVAQDGSG+YKTIN AL  VP K N TFV+++K
Sbjct: 241 -FPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIK 300

Query: 301 AGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMG 360
           AG+YKE V + K M+HL   GDGP KT+++G+ N+ DGI T++TAT + +G  F A+++G
Sbjct: 301 AGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIG 360

Query: 361 FENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN 420
           FENTAG+ KHQAVA+RVQSD SIF+NC+ DGYQDTLY H+HRQF+RDCTISGTIDF+FG+
Sbjct: 361 FENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGD 420

Query: 421 AATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAY 480
           AA  FQNC L+VRKPL NQ C +TAHGR + +E T  +FQ C   G+P YL  K  +KAY
Sbjct: 421 AAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAY 480

Query: 481 LGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWR 540
           LGRPWKEYSRTII+ + I D +QP+GW PW+G+FGL TLFY+EVQN GPG+  + RV W 
Sbjct: 481 LGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWA 540

Query: 541 GIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           GIK ++ +    FTP ++I GD WIP KG+PY++G++
Sbjct: 541 GIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of PI0007721 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.8e-187
Identity = 317/574 (55.23%), Postives = 419/574 (73.00%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEIS 60
           MS G+ + E  K+KK+  IIS+SS++L++MVVAVT+GV+      +  D+ G E   EI+
Sbjct: 1   MSYGY-DDEDAKRKKRYVIISISSVLLISMVVAVTIGVS-----VNKSDNAGDE---EIT 60

Query: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120
           +S KAI+ +C PTDYKETCE +L  +A +T+DP ELVK  F    + +++  + S T+ E
Sbjct: 61  TSVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIE 120

Query: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180
           L KDPR   AL  C+EL++YAI +L++SFE +G F+  K+DE +  L+IWLS  +++EQT
Sbjct: 121 LQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQT 180

Query: 181 CLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDDH 240
           CLDGF+ T G+AG  +++ LK+A Q+T NGL +V E+S+ LG +QIP M  RRLL     
Sbjct: 181 CLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLL---SQ 240

Query: 241 EMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAG 300
           E PSW+    RRL+ A  + +KPD+VVAQDGSG+YKTIN AL  VP K N TFV+++K G
Sbjct: 241 EFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEG 300

Query: 301 IYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 360
           IYKE V + + MTHL   GDGP KTV++GS ++ DGI T+KTAT + +G +F A+++ FE
Sbjct: 301 IYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFE 360

Query: 361 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAG+ KHQAVA+RV +D SIFYNC+ DGYQDTLYAH+HRQFYRDCTISGTIDF+FG+AA
Sbjct: 361 NTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAA 420

Query: 421 TNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYLG 480
             FQNC L+VRKPL NQ C +TAHGR + +E T  + Q C  +G+P YL  K  +K YLG
Sbjct: 421 AVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLG 480

Query: 481 RPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRGI 540
           RPWKEYSRTII+ + I D + PEGW PW+G FGLNTLFY+EVQN GPGA  +KRV W GI
Sbjct: 481 RPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGI 540

Query: 541 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGM 575
           K ++ +    FTP ++I GDAWIP KG+PY  G+
Sbjct: 541 KKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of PI0007721 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 8.3e-169
Identity = 282/571 (49.39%), Postives = 401/571 (70.23%), Query Frame = 0

Query: 9   EGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEISSSTKAIQA 68
           +G+ KKKK  I  V + +LV MVVA  VG+ ++ +  HS+         +I ++T A++A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTSRNTSHSE----KIVPVQIKTATTAVEA 63

Query: 69  ICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTT--LQELAKDPR 128
           +C PTDYKETC  SLM  + ++T P +L+K+GF +  RS+ ++I+ ++     + A D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 129 TNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFE 188
           T  AL+ C +L+  A DDL +  +    F + ++++FV DL++WLSG++ Y+QTC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 189 NTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTG------RRLLEDDDH 248
            T       MQ+  K+++++T+NGL ++  +S++LG   + G+TG      R+LL  +D 
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED- 243

Query: 249 EMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAG 308
            +PSWV    RRLM A    +K ++VVA DGSG+YKTIN AL  VP  + K FVIY+K G
Sbjct: 244 GIPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQG 303

Query: 309 IYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDG-IQTFKTATFSAIGANFYARDMGF 368
           +Y E V + K MTH+T  GDGPTKT +TGSLN+  G ++T+ TAT +  G NF A+++GF
Sbjct: 304 VYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGF 363

Query: 369 ENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 428
           ENTAG   HQAVALRV +D ++FYNCQIDGYQDTLY H+HRQF+RDCT+SGT+DF+FG+ 
Sbjct: 364 ENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDG 423

Query: 429 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYL 488
               QNC +VVRKP+ +Q C++TA GR +++E T L+ Q+CH  G+P Y+P KSINKAYL
Sbjct: 424 IVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYL 483

Query: 489 GRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRG 548
           GRPWKE+SRTII+G+ IDD+I P GWLPW G+F LNTL+YAE +N GPG+++++RVKW G
Sbjct: 484 GRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPG 543

Query: 549 IKHITPQHAADFTPRRFIDGDAWIPAKGIPY 571
           IK ++P+ A  FTP RF+ G+ WIP   +PY
Sbjct: 544 IKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of PI0007721 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 548.9 bits (1413), Expect = 6.8e-155
Identity = 268/577 (46.45%), Postives = 390/577 (67.59%), Query Frame = 0

Query: 8   AEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEISSSTKAIQ 67
           ++GDKKKK I   SVS  +   +++ V+V V ++ H    D++H       I  +TKA+Q
Sbjct: 3   SDGDKKKKFIVAGSVSGFL---VIMVVSVAVVTSKHSPKDDENH-------IRKTTKAVQ 62

Query: 68  AICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNST--TLQELAKDP 127
           A+C PTD+K+TC  SLM  + ++ DP +L+K+GFK+  +S+NE++  ++     +  K+P
Sbjct: 63  AVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNP 122

Query: 128 RTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGF 187
               A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+D F
Sbjct: 123 EAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSF 182

Query: 188 ENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTG------RRLLEDDD 247
                +    M +  K+++++++N L +V  +S+++ +  + G+TG      R+LL  +D
Sbjct: 183 GEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTED 242

Query: 248 HEMPSWVSDGKRRLMQA---GAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIY 307
             +P+WV    RRLM A   G   +K + VVAQDG+G++KTI  AL  VP  +   F+I+
Sbjct: 243 -SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIH 302

Query: 308 VKAGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNF-IDGIQTFKTATFSAIGANFYAR 367
           +K GIYKE V + K M H+T  GDGP KT++TGSLNF I  ++TF TAT +  G +F A+
Sbjct: 303 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 362

Query: 368 DMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFV 427
           ++G ENTAG    QAVALRV +D ++F++CQIDG+QDTLY H+HRQFYRDCT+SGT+DF+
Sbjct: 363 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 422

Query: 428 FGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSIN 487
           FG+A    QNCK+VVRKP   Q C+VTA GR N +E T L+   CH  GDP Y+P KS+N
Sbjct: 423 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 482

Query: 488 KAYLGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRV 547
           KAYLGRPWKE+SRTII+ + IDD+I P GWLPW G+F L TL+YAE  N GPG+++++RV
Sbjct: 483 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 542

Query: 548 KWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSS 573
           KW GIK +TPQ A  +T  RF+ GD WIP   +PY++
Sbjct: 543 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of PI0007721 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 2.3e-134
Identity = 235/452 (51.99%), Postives = 314/452 (69.47%), Query Frame = 0

Query: 131 LQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFENTTG 190
           ++ C E+L+YA+D +++S   +  F   KL E+  DLK+WL+G L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 191 DAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMT-GRRLLEDDDHEMPSWVSDG 250
            AG  M + LK++ ++++N + +++ +S IL        +  RRLL DD   +PSWV+DG
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDG--IPSWVNDG 120

Query: 251 KRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAGIYKETVVIP 310
            RRL+  G  +++P+ VVAQDGSG++KT+  AL  VP K+   FVI+VKAG+YKETV + 
Sbjct: 121 HRRLLAGG--NVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVNVA 180

Query: 311 KHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFENTAGSAKHQ 370
           K M ++T+ GDGPTKT  TGSLN+ DGI T+ TATF   GANF A+D+GFENTAG+ KHQ
Sbjct: 181 KEMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQ 240

Query: 371 AVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLV 430
           AVALRV +D++IFYNCQ+DG+QDTLY  + RQFYRDC+ISGTIDFVFG     FQNCKLV
Sbjct: 241 AVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLV 300

Query: 431 VRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYLGRPWKEYSRT 490
            R P   QQC+VTA GR  +   +AL+FQS HF G+P  L   +   +YLGRPWK+YS+ 
Sbjct: 301 CRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYSKV 360

Query: 491 IIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRGIKHITPQHAA 550
           +I+ S ID I  PEG++PWMG+    T  Y E  NKGPGAD + RVKW G+K +T   AA
Sbjct: 361 VIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAA 420

Query: 551 DFTPRRFID------GDAWIPAKGIPYSSGMM 576
           ++ P +F +       D WI   G+PYS G M
Sbjct: 421 EYYPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

BLAST of PI0007721 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 548/575 (95.30%), Postives = 563/575 (97.91%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEIS 60
           MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVG+N+AP+ EH + D GS+ TPEIS
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEIS 60

Query: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120
           SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE
Sbjct: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120

Query: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180
           L+KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT
Sbjct: 121 LSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180

Query: 181 CLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDDH 240
           CLDGFENTTGDAG RMQEFLKSAQ+MTTNGLGIVNELS+ILGSLQIPGMTGRRLLEDDDH
Sbjct: 181 CLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDDH 240

Query: 241 EMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAG 300
           EMPSW+SDGKRRLMQAGAA MKPDL+VAQDGSGKYKTINAAL DVPLKSNKTFVIYVKAG
Sbjct: 241 EMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 300

Query: 301 IYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 360
           IYKE VVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE
Sbjct: 301 IYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 360

Query: 361 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA
Sbjct: 361 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420

Query: 421 TNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYLG 480
           TNFQNCKLVVRKP+DNQQCIVTAHGR+NRKEPTALIFQ CHFMGDP YLPFKSINKAYLG
Sbjct: 421 TNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLG 480

Query: 481 RPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRGI 540
           RPWK+YSRTIIIGSQIDDIIQPEGWLPWMG+FGLNTLFYAEVQNKGPGA E+KRVKWRGI
Sbjct: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGI 540

Query: 541 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of PI0007721 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 549/576 (95.31%), Postives = 565/576 (98.09%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHG-EHSDDDHGSEATPEI 60
           MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVN+APHG EH DD+HGSE TPEI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60

Query: 61  SSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQ 120
           +SSTKAIQAICQPTDYKETCEKSL AEAGNTTDPKELVKVGFKIATRSLNEAI+NSTTL+
Sbjct: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQ 180
           ELAKDPRTNQALQNCRELLEYAIDDLN+SFERIGTFQMSKLD+FVADLKIWLSGALTYEQ
Sbjct: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDD 240
           TCLDGFENTTGDAG RMQEFLKSAQQMTTNGLGIVNELS+ILGSLQ+PGM+GRRLLEDDD
Sbjct: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDD 240

Query: 241 HEMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKA 300
            EMPSWVSDGKRRLMQAGAA+MKPDLVVAQDGSGKYKTINAAL DVPLKSNKTFVI+VKA
Sbjct: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300

Query: 301 GIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKE VVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF
Sbjct: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360

Query: 361 ENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAG+AKHQAVALRVQSDRSIF+NCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA
Sbjct: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYL 480
           ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDP YLPFK+INKAYL
Sbjct: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480

Query: 481 GRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRG 540
           GRPWKEYSRTIIIGS IDDIIQPEGWLPWMG+FGLNTLFYAEVQNKG GADESKRVKWRG
Sbjct: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of PI0007721 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.9e-278
Identity = 475/584 (81.34%), Postives = 519/584 (88.87%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSD---------DDH 60
           MSNGF +AE DKKKKKIA+ISVSSLILVAMVVAVTVGVN+AP  +  D          DH
Sbjct: 1   MSNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60

Query: 61  GSEATPEISSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEA 120
           G +A  +ISSSTKAI+ ICQPTDYKETCE+SLM EAGNTTDPKELVK GFKIA   LN A
Sbjct: 61  GDDA-EKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAA 120

Query: 121 IRNSTTLQELAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWL 180
           IRNSTTLQELA+DPR NQALQNCRELLEYAIDDL++S+ RIGTF +SK +EFV DLKIWL
Sbjct: 121 IRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWL 180

Query: 181 SGALTYEQTCLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTG 240
           S ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNGLGIV+ELS ILG+L  P  TG
Sbjct: 181 SAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATG 240

Query: 241 RRLLEDDDHEMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNK 300
           RRLL+ D   +P WVSDGKRRLMQ G  ++KPDLVVAQDGSGKYKTIN ALV VP+KSNK
Sbjct: 241 RRLLDTD---IPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNK 300

Query: 301 TFVIYVKAGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGAN 360
           TFVIYVKAGIYKE VVIPKHMTHLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+AIGAN
Sbjct: 301 TFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGAN 360

Query: 361 FYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGT 420
           F+ RDMGF NTAG  KHQAVALRVQSDR+I YNCQIDGYQDTLYAHA+RQFYRDC ISGT
Sbjct: 361 FFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGT 420

Query: 421 IDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPF 480
           IDFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+EPTALIFQ CHFMGDP YLPF
Sbjct: 421 IDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPF 480

Query: 481 KSINKAYLGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADE 540
           K +NKAYLGRPWK+YSRTII+GSQIDD+IQPEGWLPWMG+F LNTLFYAEVQN+GPGAD+
Sbjct: 481 KKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQ 540

Query: 541 SKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           S RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYSSGMM
Sbjct: 541 SHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of PI0007721 vs. ExPASy TrEMBL
Match: A0A6J1KZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 478/605 (79.01%), Postives = 524/605 (86.61%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPH------------GEHSD 60
           MSNGF +AEGDKKKKKIA+ISVSSLILVAMVVAVTVGVN+APH             +H D
Sbjct: 1   MSNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGD 60

Query: 61  D------------------DHGSEATPEISSSTKAIQAICQPTDYKETCEKSLMAEAGNT 120
           D                  DHG +   +ISSSTKAI+ ICQPTDYKETCE+SLM EAGNT
Sbjct: 61  DGGDVHDHGDYGGGGGDAHDHGDDG-EKISSSTKAIKTICQPTDYKETCEESLMVEAGNT 120

Query: 121 TDPKELVKVGFKIATRSLNEAIRNSTTLQELAKDPRTNQALQNCRELLEYAIDDLNESFE 180
           TD KELVK GFKIA   LN AIRNSTTLQELAKDPR NQALQNCRELLEYAIDDL++S+ 
Sbjct: 121 TDLKELVKAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYH 180

Query: 181 RIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFENTTGDAGARMQEFLKSAQQMTTNG 240
           RIGTF +SK +EFV DLKIWLS ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNG
Sbjct: 181 RIGTFHVSKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNG 240

Query: 241 LGIVNELSSILGSLQIPGMTGRRLLEDDDHEMPSWVSDGKRRLMQAGAASMKPDLVVAQD 300
           LGIV+ELS+ILG+L  P  TGRRLL+ D   MP WVSDGKRRLMQ    ++KPDLVVAQD
Sbjct: 241 LGIVSELSNILGTLHEPAATGRRLLDTD---MPLWVSDGKRRLMQLETGAIKPDLVVAQD 300

Query: 301 GSGKYKTINAALVDVPLKSNKTFVIYVKAGIYKETVVIPKHMTHLTMYGDGPTKTVVTGS 360
           GSGKYKTIN ALVDVP+KSNKTFVIYVKAGIYKE VVIPKHMTHLTMYGDGPTK+VVTG+
Sbjct: 301 GSGKYKTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGN 360

Query: 361 LNFIDGIQTFKTATFSAIGANFYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGY 420
           LNF+DGIQTFKTATF+AIGANF+ RDMGF N+AG  KHQAVALR+QSDR+I YNCQIDG+
Sbjct: 361 LNFVDGIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGF 420

Query: 421 QDTLYAHAHRQFYRDCTISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRK 480
           QDTLYAHA+RQFYRDCTISGTIDFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+
Sbjct: 421 QDTLYAHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRR 480

Query: 481 EPTALIFQSCHFMGDPGYLPFKSINKAYLGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMG 540
           EPTALIFQ CHFMGDP YLPFK +NKAYLGRPWK+YSRTII+GSQIDD+IQPEGWLPWMG
Sbjct: 481 EPTALIFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMG 540

Query: 541 NFGLNTLFYAEVQNKGPGADESKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPY 576
           +FGLNTLFYAEVQN+GPGAD+S RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PY
Sbjct: 541 DFGLNTLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPY 600

BLAST of PI0007721 vs. ExPASy TrEMBL
Match: A0A6J1KJ31 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 8.4e-257
Identity = 440/576 (76.39%), Postives = 496/576 (86.11%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPH-GEHSDDDHGSEATPEI 60
           MSNGF +AEGD KKKK+A + VSSL LVAMVVAVTVGVN++ H GE   D HG +   EI
Sbjct: 1   MSNGF-DAEGDNKKKKMAFVGVSSLFLVAMVVAVTVGVNNSSHKGEGLGDGHG-DGAGEI 60

Query: 61  SSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQ 120
           SSSTKAIQ IC+PTDYKETCEKSLM+E  NTTDP+ELVK+GFK+    LN AIRNSTTLQ
Sbjct: 61  SSSTKAIQTICRPTDYKETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQ 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQ 180
            LAK+PRTNQALQNCRELLEYAIDD+ +SF RIGTFQM+KLD+FV DL IWLSGALTYEQ
Sbjct: 121 ALAKEPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDD 240
           TCLDGFENTTGDAG RM+ FLKSAQ+MT+NGL IV EL ++LGSLQ     GRRLL  D 
Sbjct: 181 TCLDGFENTTGDAGERMKMFLKSAQEMTSNGLAIVTELKNMLGSLQ-GSKAGRRLLAGD- 240

Query: 241 HEMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKA 300
            E+PSWVS  KRRLMQ   +++ P++VVAQDGSGKYKTIN ALVDVP KSN TFVIYVKA
Sbjct: 241 -EVPSWVSSEKRRLMQLPRSAITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKA 300

Query: 301 GIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKETV+IPK +TH+TMYGDGPTKT+V+G+LNF+DGIQTFKTATF+ +GANF+ RDMGF
Sbjct: 301 GIYKETVLIPKALTHVTMYGDGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGF 360

Query: 361 ENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAGSAKHQAVALRVQSDRSIF+NCQ+DGYQDTLYA  HRQFYRDC +SGTIDFVFGN+
Sbjct: 361 ENTAGSAKHQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNS 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYL 480
           A  FQNC LVVRKPL NQQCIVTA GRL  +EPT  +FQ CH MGDP YLPFK++NKAYL
Sbjct: 421 AAVFQNCNLVVRKPLANQQCIVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYL 480

Query: 481 GRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRG 540
           GRPWK+YSRTII+GS+IDD+IQPEGWLPWMG+FGLN+LFYAE QNKGPGAD + RVKW+G
Sbjct: 481 GRPWKQYSRTIIMGSEIDDLIQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           IK +T Q AADFTP+ FIDGDAWI   G+PYS GMM
Sbjct: 541 IKTLTAQQAADFTPKVFIDGDAWILNTGVPYSPGMM 571

BLAST of PI0007721 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 548/575 (95.30%), Postives = 563/575 (97.91%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEIS 60
           MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVG+N+AP+ EH + D GS+ TPEIS
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPYEEHGEGDQGSKTTPEIS 60

Query: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120
           SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE
Sbjct: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120

Query: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180
           L+KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT
Sbjct: 121 LSKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180

Query: 181 CLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDDH 240
           CLDGFENTTGDAG RMQEFLKSAQ+MTTNGLGIVNELS+ILGSLQIPGMTGRRLLEDDDH
Sbjct: 181 CLDGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQIPGMTGRRLLEDDDH 240

Query: 241 EMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAG 300
           EMPSW+SDGKRRLMQAGAA MKPDL+VAQDGSGKYKTINAAL DVPLKSNKTFVIYVKAG
Sbjct: 241 EMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 300

Query: 301 IYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 360
           IYKE VVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE
Sbjct: 301 IYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 360

Query: 361 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA
Sbjct: 361 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420

Query: 421 TNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYLG 480
           TNFQNCKLVVRKP+DNQQCIVTAHGR+NRKEPTALIFQ CHFMGDP YLPFKSINKAYLG
Sbjct: 421 TNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKAYLG 480

Query: 481 RPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRGI 540
           RPWK+YSRTIIIGSQIDDIIQPEGWLPWMG+FGLNTLFYAEVQNKGPGA E+KRVKWRGI
Sbjct: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKWRGI 540

Query: 541 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

BLAST of PI0007721 vs. NCBI nr
Match: XP_004150503.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.1 hypothetical protein Csa_021732 [Cucumis sativus])

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 549/576 (95.31%), Postives = 565/576 (98.09%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHG-EHSDDDHGSEATPEI 60
           MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVN+APHG EH DD+HGSE TPEI
Sbjct: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEI 60

Query: 61  SSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQ 120
           +SSTKAIQAICQPTDYKETCEKSL AEAGNTTDPKELVKVGFKIATRSLNEAI+NSTTL+
Sbjct: 61  TSSTKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLK 120

Query: 121 ELAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQ 180
           ELAKDPRTNQALQNCRELLEYAIDDLN+SFERIGTFQMSKLD+FVADLKIWLSGALTYEQ
Sbjct: 121 ELAKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQ 180

Query: 181 TCLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDD 240
           TCLDGFENTTGDAG RMQEFLKSAQQMTTNGLGIVNELS+ILGSLQ+PGM+GRRLLEDDD
Sbjct: 181 TCLDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQLPGMSGRRLLEDDD 240

Query: 241 HEMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKA 300
            EMPSWVSDGKRRLMQAGAA+MKPDLVVAQDGSGKYKTINAAL DVPLKSNKTFVI+VKA
Sbjct: 241 REMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKA 300

Query: 301 GIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360
           GIYKE VVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF
Sbjct: 301 GIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGF 360

Query: 361 ENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420
           ENTAG+AKHQAVALRVQSDRSIF+NCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA
Sbjct: 361 ENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 420

Query: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYL 480
           ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDP YLPFK+INKAYL
Sbjct: 421 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYL 480

Query: 481 GRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRG 540
           GRPWKEYSRTIIIGS IDDIIQPEGWLPWMG+FGLNTLFYAEVQNKG GADESKRVKWRG
Sbjct: 481 GRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRG 540

Query: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 IKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of PI0007721 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 532/575 (92.52%), Postives = 552/575 (96.00%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEIS 60
           MSNGFANAEGDKKKKK+AIISVSSLILVAMVVAVTVGVN+AP  EH  D HG E T EIS
Sbjct: 1   MSNGFANAEGDKKKKKMAIISVSSLILVAMVVAVTVGVNNAPKEEHG-DGHGGE-THEIS 60

Query: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120
           S+TKAIQAICQPTDYK+ CEK+LMAEAGNTTDPKEL KVG +IATR+LNEAIRNSTTLQE
Sbjct: 61  STTKAIQAICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQE 120

Query: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180
           LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT
Sbjct: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180

Query: 181 CLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDDH 240
           CLDGFENTTGDAG RMQEFLKS+QQMTTNGL IVNELS +LGSLQIPGMTGRRLLEDD  
Sbjct: 181 CLDGFENTTGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSLQIPGMTGRRLLEDDAD 240

Query: 241 EMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAG 300
           EMPSWVS+GKRRLMQA AASMKPDLVVAQDGSGKYKTINAAL DVPLKSNKTFVIYVKAG
Sbjct: 241 EMPSWVSEGKRRLMQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYVKAG 300

Query: 301 IYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 360
           IYKE VVIPK MTHLTMYGDGPTKT+VTGSLNFIDGIQTFKTATF+AIGANFYARDMGFE
Sbjct: 301 IYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATFAAIGANFYARDMGFE 360

Query: 361 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAG+AKHQAVALRVQSDRSIFYNC+IDGYQDTLYAHAHRQFYRDC ISGTIDFVFGNAA
Sbjct: 361 NTAGAAKHQAVALRVQSDRSIFYNCKIDGYQDTLYAHAHRQFYRDCIISGTIDFVFGNAA 420

Query: 421 TNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYLG 480
           T FQNCKLVVRKP+DNQQCIVTAHGRLNRKEPTALIFQSCHFMGDP YLPFK+INKAYLG
Sbjct: 421 TIFQNCKLVVRKPMDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKNINKAYLG 480

Query: 481 RPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRGI 540
           RPWK+YSRTIIIGSQIDDIIQPEGWLPWMG+FGLNTLFYAEVQNKGPGADESKRVKWRGI
Sbjct: 481 RPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGADESKRVKWRGI 540

Query: 541 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           +HITPQHAAD+TPRRFIDGDAWIPAKGIPYSSGMM
Sbjct: 541 RHITPQHAADYTPRRFIDGDAWIPAKGIPYSSGMM 573

BLAST of PI0007721 vs. NCBI nr
Match: XP_023549205.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 971.5 bits (2510), Expect = 3.3e-279
Identity = 476/584 (81.51%), Postives = 521/584 (89.21%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSD---------DDH 60
           MSNGF +AEGDKKKKKIA+ISVSSLILVAMVVAVTVGVN+AP  +  D          DH
Sbjct: 1   MSNGF-DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60

Query: 61  GSEATPEISSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEA 120
           G +   ++SSSTKAI+ ICQPTDYKETCE+SLM EAGNTTDPKELVK GFKIA   LN A
Sbjct: 61  GDDG-EKVSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAA 120

Query: 121 IRNSTTLQELAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWL 180
           IRNSTTLQELA+DPR NQALQNCRELLEYAIDDL++S+ RIGTF +SK +EFV DLKIWL
Sbjct: 121 IRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWL 180

Query: 181 SGALTYEQTCLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTG 240
           S ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNGLGIV+ELS+ILG+L  P  TG
Sbjct: 181 SAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSNILGTLHAPAATG 240

Query: 241 RRLLEDDDHEMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNK 300
           RRLL+ D   MP WVSDGKRRLMQ G  ++KPDLVVAQDGSGKYKTIN ALVDVP+KSNK
Sbjct: 241 RRLLDTD---MPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVDVPMKSNK 300

Query: 301 TFVIYVKAGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGAN 360
           TFVIYVKAGIYKE VVIPKHMTHLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+AIGAN
Sbjct: 301 TFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGAN 360

Query: 361 FYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGT 420
           F+ RDMGF NTAG  KHQAVALRVQSDR+I YNCQIDGYQDTLYAHA+RQFYRDC ISGT
Sbjct: 361 FFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGT 420

Query: 421 IDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPF 480
           IDFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+EPTALIFQ CHFMGDP YLPF
Sbjct: 421 IDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPAYLPF 480

Query: 481 KSINKAYLGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADE 540
           K +NKAYLGRPWK+YSRTII+GSQIDD+IQPEGWLPWMG+F LNTLFYAEVQN+GPGAD+
Sbjct: 481 KKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQ 540

Query: 541 SKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           S RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYSSGMM
Sbjct: 541 SHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of PI0007721 vs. NCBI nr
Match: XP_022959432.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata])

HSP 1 Score: 966.8 bits (2498), Expect = 8.0e-278
Identity = 475/584 (81.34%), Postives = 519/584 (88.87%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSD---------DDH 60
           MSNGF +AE DKKKKKIA+ISVSSLILVAMVVAVTVGVN+AP  +  D          DH
Sbjct: 1   MSNGF-DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDH 60

Query: 61  GSEATPEISSSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEA 120
           G +A  +ISSSTKAI+ ICQPTDYKETCE+SLM EAGNTTDPKELVK GFKIA   LN A
Sbjct: 61  GDDA-EKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAA 120

Query: 121 IRNSTTLQELAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWL 180
           IRNSTTLQELA+DPR NQALQNCRELLEYAIDDL++S+ RIGTF +SK +EFV DLKIWL
Sbjct: 121 IRNSTTLQELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWL 180

Query: 181 SGALTYEQTCLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTG 240
           S ALTYEQTCLDGFENTTGD+G RM+EFLKSAQ+MTTNGLGIV+ELS ILG+L  P  TG
Sbjct: 181 SAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLDAPAATG 240

Query: 241 RRLLEDDDHEMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNK 300
           RRLL+ D   +P WVSDGKRRLMQ G  ++KPDLVVAQDGSGKYKTIN ALV VP+KSNK
Sbjct: 241 RRLLDTD---IPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNK 300

Query: 301 TFVIYVKAGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGAN 360
           TFVIYVKAGIYKE VVIPKHMTHLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+AIGAN
Sbjct: 301 TFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGAN 360

Query: 361 FYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGT 420
           F+ RDMGF NTAG  KHQAVALRVQSDR+I YNCQIDGYQDTLYAHA+RQFYRDC ISGT
Sbjct: 361 FFVRDMGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGT 420

Query: 421 IDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPF 480
           IDFVFGN+A  FQNCKLVVRKPLDNQQCIVTAHGRLNR+EPTALIFQ CHFMGDP YLPF
Sbjct: 421 IDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPF 480

Query: 481 KSINKAYLGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADE 540
           K +NKAYLGRPWK+YSRTII+GSQIDD+IQPEGWLPWMG+F LNTLFYAEVQN+GPGAD+
Sbjct: 481 KKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQ 540

Query: 541 SKRVKWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           S RVKWRGI+HITPQHAA +T RRFIDGDAWIP +G+PYSSGMM
Sbjct: 541 SHRVKWRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

BLAST of PI0007721 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 659.1 bits (1699), Expect = 3.3e-189
Identity = 308/577 (53.38%), Postives = 426/577 (73.83%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEIS 60
           MS G+   +  K+K++  +I++SS++L++MVVAVTVGV+   H      D  S+   E++
Sbjct: 1   MSYGY--DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKH------DGDSKGKAEVN 60

Query: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120
           +S KA++ +C PTDY++TCE +L+    NTTDP ELVK  F +  + + +A + S T+ E
Sbjct: 61  ASVKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIME 120

Query: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180
           L KD RT  AL  C+EL++YA+D+L+ SFE +G F+   LDE + +L+IWLS A+++E+T
Sbjct: 121 LQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEET 180

Query: 181 CLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDDH 240
           CL+GF+ T G+AG  M++ LK+A ++T NGL I++E+S+ +G +QIPG+  RRLL +   
Sbjct: 181 CLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEG-- 240

Query: 241 EMPSWVSDGKRRLMQAGAA--SMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVK 300
             PSWV    R+L+QA AA   +KPD+VVAQDGSG+YKTIN AL  VP K N TFV+++K
Sbjct: 241 -FPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIK 300

Query: 301 AGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMG 360
           AG+YKE V + K M+HL   GDGP KT+++G+ N+ DGI T++TAT + +G  F A+++G
Sbjct: 301 AGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIG 360

Query: 361 FENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGN 420
           FENTAG+ KHQAVA+RVQSD SIF+NC+ DGYQDTLY H+HRQF+RDCTISGTIDF+FG+
Sbjct: 361 FENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGD 420

Query: 421 AATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAY 480
           AA  FQNC L+VRKPL NQ C +TAHGR + +E T  +FQ C   G+P YL  K  +KAY
Sbjct: 421 AAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAY 480

Query: 481 LGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWR 540
           LGRPWKEYSRTII+ + I D +QP+GW PW+G+FGL TLFY+EVQN GPG+  + RV W 
Sbjct: 481 LGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWA 540

Query: 541 GIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576
           GIK ++ +    FTP ++I GD WIP KG+PY++G++
Sbjct: 541 GIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

BLAST of PI0007721 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 657.1 bits (1694), Expect = 1.3e-188
Identity = 317/574 (55.23%), Postives = 419/574 (73.00%), Query Frame = 0

Query: 1   MSNGFANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEIS 60
           MS G+ + E  K+KK+  IIS+SS++L++MVVAVT+GV+      +  D+ G E   EI+
Sbjct: 1   MSYGY-DDEDAKRKKRYVIISISSVLLISMVVAVTIGVS-----VNKSDNAGDE---EIT 60

Query: 61  SSTKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQE 120
           +S KAI+ +C PTDYKETCE +L  +A +T+DP ELVK  F    + +++  + S T+ E
Sbjct: 61  TSVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIE 120

Query: 121 LAKDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQT 180
           L KDPR   AL  C+EL++YAI +L++SFE +G F+  K+DE +  L+IWLS  +++EQT
Sbjct: 121 LQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQT 180

Query: 181 CLDGFENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTGRRLLEDDDH 240
           CLDGF+ T G+AG  +++ LK+A Q+T NGL +V E+S+ LG +QIP M  RRLL     
Sbjct: 181 CLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLL---SQ 240

Query: 241 EMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAG 300
           E PSW+    RRL+ A  + +KPD+VVAQDGSG+YKTIN AL  VP K N TFV+++K G
Sbjct: 241 EFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEG 300

Query: 301 IYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 360
           IYKE V + + MTHL   GDGP KTV++GS ++ DGI T+KTAT + +G +F A+++ FE
Sbjct: 301 IYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFE 360

Query: 361 NTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 420
           NTAG+ KHQAVA+RV +D SIFYNC+ DGYQDTLYAH+HRQFYRDCTISGTIDF+FG+AA
Sbjct: 361 NTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAA 420

Query: 421 TNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYLG 480
             FQNC L+VRKPL NQ C +TAHGR + +E T  + Q C  +G+P YL  K  +K YLG
Sbjct: 421 AVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLG 480

Query: 481 RPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRGI 540
           RPWKEYSRTII+ + I D + PEGW PW+G FGLNTLFY+EVQN GPGA  +KRV W GI
Sbjct: 481 RPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGI 540

Query: 541 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGM 575
           K ++ +    FTP ++I GDAWIP KG+PY  G+
Sbjct: 541 KKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of PI0007721 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 595.1 bits (1533), Expect = 5.9e-170
Identity = 282/571 (49.39%), Postives = 401/571 (70.23%), Query Frame = 0

Query: 9   EGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEISSSTKAIQA 68
           +G+ KKKK  I  V + +LV MVVA  VG+ ++ +  HS+         +I ++T A++A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTSRNTSHSE----KIVPVQIKTATTAVEA 63

Query: 69  ICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTT--LQELAKDPR 128
           +C PTDYKETC  SLM  + ++T P +L+K+GF +  RS+ ++I+ ++     + A D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 129 TNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFE 188
           T  AL+ C +L+  A DDL +  +    F + ++++FV DL++WLSG++ Y+QTC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 189 NTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTG------RRLLEDDDH 248
            T       MQ+  K+++++T+NGL ++  +S++LG   + G+TG      R+LL  +D 
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED- 243

Query: 249 EMPSWVSDGKRRLMQAGAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIYVKAG 308
            +PSWV    RRLM A    +K ++VVA DGSG+YKTIN AL  VP  + K FVIY+K G
Sbjct: 244 GIPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQG 303

Query: 309 IYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDG-IQTFKTATFSAIGANFYARDMGF 368
           +Y E V + K MTH+T  GDGPTKT +TGSLN+  G ++T+ TAT +  G NF A+++GF
Sbjct: 304 VYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGF 363

Query: 369 ENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNA 428
           ENTAG   HQAVALRV +D ++FYNCQIDGYQDTLY H+HRQF+RDCT+SGT+DF+FG+ 
Sbjct: 364 ENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDG 423

Query: 429 ATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSINKAYL 488
               QNC +VVRKP+ +Q C++TA GR +++E T L+ Q+CH  G+P Y+P KSINKAYL
Sbjct: 424 IVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYL 483

Query: 489 GRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRVKWRG 548
           GRPWKE+SRTII+G+ IDD+I P GWLPW G+F LNTL+YAE +N GPG+++++RVKW G
Sbjct: 484 GRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPG 543

Query: 549 IKHITPQHAADFTPRRFIDGDAWIPAKGIPY 571
           IK ++P+ A  FTP RF+ G+ WIP   +PY
Sbjct: 544 IKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of PI0007721 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 548.9 bits (1413), Expect = 4.9e-156
Identity = 268/577 (46.45%), Postives = 390/577 (67.59%), Query Frame = 0

Query: 8   AEGDKKKKKIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEISSSTKAIQ 67
           ++GDKKKK I   SVS  +   +++ V+V V ++ H    D++H       I  +TKA+Q
Sbjct: 3   SDGDKKKKFIVAGSVSGFL---VIMVVSVAVVTSKHSPKDDENH-------IRKTTKAVQ 62

Query: 68  AICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNST--TLQELAKDP 127
           A+C PTD+K+TC  SLM  + ++ DP +L+K+GFK+  +S+NE++  ++     +  K+P
Sbjct: 63  AVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNP 122

Query: 128 RTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGF 187
               A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+D F
Sbjct: 123 EAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSF 182

Query: 188 ENTTGDAGARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIPGMTG------RRLLEDDD 247
                +    M +  K+++++++N L +V  +S+++ +  + G+TG      R+LL  +D
Sbjct: 183 GEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTED 242

Query: 248 HEMPSWVSDGKRRLMQA---GAASMKPDLVVAQDGSGKYKTINAALVDVPLKSNKTFVIY 307
             +P+WV    RRLM A   G   +K + VVAQDG+G++KTI  AL  VP  +   F+I+
Sbjct: 243 -SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIH 302

Query: 308 VKAGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNF-IDGIQTFKTATFSAIGANFYAR 367
           +K GIYKE V + K M H+T  GDGP KT++TGSLNF I  ++TF TAT +  G +F A+
Sbjct: 303 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 362

Query: 368 DMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFV 427
           ++G ENTAG    QAVALRV +D ++F++CQIDG+QDTLY H+HRQFYRDCT+SGT+DF+
Sbjct: 363 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 422

Query: 428 FGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPGYLPFKSIN 487
           FG+A    QNCK+VVRKP   Q C+VTA GR N +E T L+   CH  GDP Y+P KS+N
Sbjct: 423 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 482

Query: 488 KAYLGRPWKEYSRTIIIGSQIDDIIQPEGWLPWMGNFGLNTLFYAEVQNKGPGADESKRV 547
           KAYLGRPWKE+SRTII+ + IDD+I P GWLPW G+F L TL+YAE  N GPG+++++RV
Sbjct: 483 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 542

Query: 548 KWRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSS 573
           KW GIK +TPQ A  +T  RF+ GD WIP   +PY++
Sbjct: 543 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of PI0007721 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 463.4 bits (1191), Expect = 2.7e-130
Identity = 259/705 (36.74%), Postives = 377/705 (53.48%), Query Frame = 0

Query: 16  KIAIISVSSLILVAMVVAVTVGVNSAPHGEHSDDDHGSEATPEISSSTKAIQAICQPTDY 75
           K  ++ V++LI+ AMV+ V     ++P      ++   E+   I+ +T +I  IC  TDY
Sbjct: 3   KYVLLGVTALIM-AMVICVEANDGNSP--SRKMEEVHRESKLMITKTTVSI--ICASTDY 62

Query: 76  KETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAI-RNSTTLQELA-KDPRTNQALQN 135
           K+ C  SL      + DP+ L++  F +A  S+   I R    L+  A  D  T +AL  
Sbjct: 63  KQDCTTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNT 122

Query: 136 CRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCLDGFENTTGDAG 195
           CREL++ AIDDL ++ ++   F  ++L +FV DL +WLSG++TY+QTC+DGFE    +A 
Sbjct: 123 CRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAA 182

Query: 196 ARMQEFLKSAQQMTTNGLGIVNELSSILGSLQIP-------------------------- 255
             M+  ++  Q +T+NGL I   L  +L + +IP                          
Sbjct: 183 VMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGS 242

Query: 256 ------------------------------------------------------------ 315
                                                                       
Sbjct: 243 SQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKK 302

Query: 316 -GMTGRRLLED------------------------------------------------- 375
              +G + L+D                                                 
Sbjct: 303 SAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDS 362

Query: 376 -------DDHEMPSWVSDGKRRLMQAGAAS--MKPDLVVAQDGSGKYKTINAALVDVPLK 435
                  ++ E P WV+   RRL+     +  +K ++VVA+DGSGK KTI  AL  VP+K
Sbjct: 363 LKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMK 422

Query: 436 SNKTFVIYVKAGIYKETVVIPKHMTHLTMYGDGPTKTVVTGSLNFI-DGIQTFKTATFSA 495
           + K FVI++K G+YKE V + K M H+   GDGPTKTV+TG + F+ D + T++TA+ + 
Sbjct: 423 NTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAV 482

Query: 496 IGANFYARDMGFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCT 555
            G  F A+D+GFENTAG+A+HQAVALRV +D ++F+NC ++GYQDTLY H HRQFYR+C 
Sbjct: 483 NGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCR 542

Query: 556 ISGTIDFVFGNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPG 573
           +SGTIDFVFG+A   FQNC+ V+R+P+++QQCIVTA GR +R+E T ++  +    GD  
Sbjct: 543 VSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDAS 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GX864.7e-18853.38Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z81.8e-18755.23Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ218.3e-16949.39Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA16.8e-15546.45Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
Q429202.3e-13451.99Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C7B60.0e+0095.30Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1[more]
A0A0A0KD240.0e+0095.31Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1[more]
A0A6J1H4H83.9e-27881.34Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1[more]
A0A6J1KZN01.1e-27779.01Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1[more]
A0A6J1KJ318.4e-25776.39Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008458409.10.0e+0095.30PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo][more]
XP_004150503.10.0e+0095.31probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.... [more]
XP_038875843.10.0e+0092.52probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida][more]
XP_023549205.13.3e-27981.51probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo][more]
XP_022959432.18.0e-27881.34probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G05610.13.3e-18953.38Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.11.3e-18855.23Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.15.9e-17049.39Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.14.9e-15646.45Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.12.7e-13036.74Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 61..213
e-value: 1.2E-36
score: 137.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 61..217
e-value: 2.7E-25
score: 86.7
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 65..212
e-value: 1.2E-26
score: 93.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 258..574
e-value: 2.0E-117
score: 394.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 60..218
e-value: 6.1E-34
score: 119.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 64..218
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 264..560
e-value: 1.9E-135
score: 450.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..63
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 19..573
NoneNo IPR availablePANTHERPTHR31707:SF131PECTINESTERASEcoord: 19..573
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 68..219
e-value: 3.73577E-46
score: 157.6
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 408..417
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 289..308
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 261..573

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0007721.1PI0007721.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0030261 chromosome condensation
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity