PI0007530 (gene) Melon (PI 482460) v1

Overview
NamePI0007530
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionEukaryotic aspartyl protease family protein
Locationchr10: 16984865 .. 16988319 (-)
RNA-Seq ExpressionPI0007530
SyntenyPI0007530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAAATAAAACCCAAATCCTTAACTTTCAAAGGTCCCATTCTGATTTTCTAAACCCCAAAAAATTTTAAACCATGTTCTTTAAACAACTCTCTTCTCTTCTCTTCTTTCTTGCTGTCGTGGTCGCCGCCGTCGCCCGTCCGTCTTCTCCAACAAAGTTTCAAAACCTTAATGTTAAAGCTACCAAATTGGACTTCAATGACGGTCAGATTCTTCATACCCTTAATTTCTCCGACGGTCACCGGCAAGTTTCCGGTCATAAATCCCACAACAATACATTTAAACTCAACCTTCTCCATAGGGATAAACTGTCCCATGTTCATGGTCACCGTCATGGGTTTAATGACCGCATGAAAAGAGACGCCATAAGAGTAGCTACCCTCGTCCGCCGCCTATCCCACGGCGGCGCTCACGGAGCCGCCGCCGTGGAAGATAGCCGTTACAAGGTGGCTAATTTTGCCACGGATGTCATTTCAGGCAAGGAAATTTTTTATTTTTATTATTATTTAAAAAAAATACCCTTTAAAATCCAATGTGTATATTATTTTCTTGAATTGTTTTTATGATTTTTTTTTTTAATTTGGTTAAATTGGATTCGGAAATAGGAATGGAAGAGGGGAGTGGAGAATATTTTGTTCGGATCGGAGTTGGGAGCCCACCGAGGAGTCAATATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTCCAATGTAAACCTTGTAGCCGATGCTACCAACAATCCGACCCGGTTTTCGACCCGGCCGACTCCTCCTCCTTCGCCGGCGTATCTTGTGGCTCCGCCGTTTGTGACCGCCTTGAGAACACCGGCTGTAACGCTGGACGATGTCGGTACGAAGTGTCCTATGGGGACGGGTCTTACACTAAAGGCACTCTCGCTCTTGAAACTCTCACCGTCGGCGAAGTAAGCACCATAACTCAAAAACTTAAGGAATAAGTTATGATATATTTAATTATTTATTCATATTTTAAGCTTAAAACTTAAATATCACAACAAGTTAATTAATATTGTGATTTAAGCATGTCAATATATAATAATAATGTATTATAAGTTCGGTTTTTTATAGAATGTTTAAAGTGTTGTCGGTTGTGATGTTCTATGTTTAGAGTACTAACTATTTTCAATTTGAATTATAATAAGTTAATTATTTGTTGTAACAGTGAGAAACGTCAATTATTATAATTAAAACTCTAAATATAAAGTGACAGTCAAACACACCTTAACTTTTAAAATTTATAGAAACTTTAACAAACTTGAAAAAAAAAATAGGATGTGTTATGCACTTCAAATTTCGTAAGAAAGGAGCATTTAAAATATTTTTGTAAAGTTAAAAACCTTTAAAATACTTTTTTTTTTTTTTTTGAAAAAAGGAAAATTAAAGGTCTAATTTAATATTTTGATTCTTTTTCTAAAACAAGTTTCATGGTCTTTTTGTGACGTAATCATCCAACCACTACAAAGAGGGGAAAAATGATACATAAAGTTGTCACGTGAAGGAAGTTTGTTTTAAGTTTGTGCTTAAAAATCATTCGAGTAAATATTTTTGTTTTAAGAAAAATACATTAACTCAAAAATTTGTCTACAAATTCTAATCTATTGAATTTTTAATTGATAGATCTATTTGGGTAAGTCACTAATTTTTCATTAGATTTTTGTGTTTAATAAATATCTAATATTTTTGAAATTTTTTCAAACTAATTGGTCTATATGAAAATAAAAATATGATTTATTGCTTAAAAATTTCATAGAGTTAAATATGTAAATTACTTATTAGATATAAAAGTGAAAATTTAACAAACTGTTTGGTAACATTTAGTTTTTGAAACTTAAACATAAAAATATTTCATCCACTTCTTACAACGATATTTTGAAAACTTATATATAGTTTTTTTAAGTAGAATTTGGCTAACTATTTTACTCAAGTAACTAAAGACTTAAAAATATATAGAAAGATTTTGCGAGAAAATAGACTTATTTTTAGATACATTAATATCAAATGATTACGATCAAATTATATCTAAGGAAATATTTAATATAAGATTGAAAGTTTTTTGACATATTAAACACTTTTTGAATGTCAAGGATCTACTCAAACACTCATGCAAAGTTACATGAACATGTCGTAACTAACATAAATTTATACTATCAATTTCGTGGTTGGATGTTTGAGGACTAGGCAAAACAATTTAGATAGTGAGACTAGTAGTGAACATGACAAACCATAAGAAATTGAATCAATTGACTGAACTGAAAGCAATTGGTTGGAGATAAAGAAGGGTTCAGTCGACGTCGATTTAAAAAAATCCAAATCAACAACATTTTAAAAAAAATTCAAAAATTCAAACCGACCTCCTTCATGGTCGGGTTGATTTGAAGACTTACTAAATTGTCCTAATTAGTTAGAGGGCGATTTGGTTGAAAAATCGATTCCAACTGACCGTTGCTCAATGTGGGTTGGGTTTTTAAGTTACAGAAAATTTTGATGCTTAGGTTTTTGTATTTTATAATTTTATTAGGTGATGATTCGTGACGTGGCAATTGGGTGCGGTCATACAAACCAAGGAATGTTTATAGGCGCCGCCGGTTTACTCGGACTCGGTGGAGGGTCAATGTCCTTCATCGGCCAACTCGGTGGTCAAACCGGGGGAGCATTCAGCTATTGTTTAGTGAGTCGAGGCACCGGCTCAACCGGAACATTAGAATTCGGCCGTGGAGCATTGCCAGTTGGCGCCACGTGGATCTCCCTCATTCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCATCGGCGTCGGCGGAGCTCGAGTTTCAATACCAGAAGAAACGTTTCAACTCACCGAGTTCGGTACCAACGGCGTCGTAATGGACACTGGCACCGCCGTGACACGTCTTCCGACTGCGGCCTACGTGGCGTTCCGTGATTCGTTCACAGCCCAAACCAGCAACCTCCCACGCGCGGCTGGCGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAGTCCGTACGAGTGCCAACGGTGTCGTTTTACTTCTCTGATGGACCGGTTCTGACATTGCCAGCGAAGAACTTTTTGATTCCGGTAGACGGCGGCGGAACTTTTTGCTTGGCTTTTGCTCCGTCGCCATCGGGACTTTCTATAATCGGAAACATCCAGCAAGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAAATGTGCAAATTATATAATTAAGGGTTTGGAATATAGTTTTTCTTAATCACATTATTATTTTTGTTTTAAAGCTAATAGATAGATCACATGTAAATTATTTACTTCCCATTTAGTATATGCTATATACTTTCTATGAATGTTGTATTTTTCTTATTTTCAATCTAATAAAAACTACGATGGACGACCTGGTTT

mRNA sequence

CTCAAATAAAACCCAAATCCTTAACTTTCAAAGGTCCCATTCTGATTTTCTAAACCCCAAAAAATTTTAAACCATGTTCTTTAAACAACTCTCTTCTCTTCTCTTCTTTCTTGCTGTCGTGGTCGCCGCCGTCGCCCGTCCGTCTTCTCCAACAAAGTTTCAAAACCTTAATGTTAAAGCTACCAAATTGGACTTCAATGACGGTCAGATTCTTCATACCCTTAATTTCTCCGACGGTCACCGGCAAGTTTCCGGTCATAAATCCCACAACAATACATTTAAACTCAACCTTCTCCATAGGGATAAACTGTCCCATGTTCATGGTCACCGTCATGGGTTTAATGACCGCATGAAAAGAGACGCCATAAGAGTAGCTACCCTCGTCCGCCGCCTATCCCACGGCGGCGCTCACGGAGCCGCCGCCGTGGAAGATAGCCGTTACAAGGTGGCTAATTTTGCCACGGATGTCATTTCAGGAATGGAAGAGGGGAGTGGAGAATATTTTGTTCGGATCGGAGTTGGGAGCCCACCGAGGAGTCAATATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTCCAATGTAAACCTTGTAGCCGATGCTACCAACAATCCGACCCGGTTTTCGACCCGGCCGACTCCTCCTCCTTCGCCGGCGTATCTTGTGGCTCCGCCGTTTGTGACCGCCTTGAGAACACCGGCTGTAACGCTGGACGATGTCGGTACGAAGTGTCCTATGGGGACGGGTCTTACACTAAAGGCACTCTCGCTCTTGAAACTCTCACCGTCGGCGAAGTGATGATTCGTGACGTGGCAATTGGGTGCGGTCATACAAACCAAGGAATGTTTATAGGCGCCGCCGGTTTACTCGGACTCGGTGGAGGGTCAATGTCCTTCATCGGCCAACTCGGTGGTCAAACCGGGGGAGCATTCAGCTATTGTTTAGTGAGTCGAGGCACCGGCTCAACCGGAACATTAGAATTCGGCCGTGGAGCATTGCCAGTTGGCGCCACGTGGATCTCCCTCATTCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCATCGGCGTCGGCGGAGCTCGAGTTTCAATACCAGAAGAAACGTTTCAACTCACCGAGTTCGGTACCAACGGCGTCGTAATGGACACTGGCACCGCCGTGACACGTCTTCCGACTGCGGCCTACGTGGCGTTCCGTGATTCGTTCACAGCCCAAACCAGCAACCTCCCACGCGCGGCTGGCGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAGTCCGTACGAGTGCCAACGGTGTCGTTTTACTTCTCTGATGGACCGGTTCTGACATTGCCAGCGAAGAACTTTTTGATTCCGGTAGACGGCGGCGGAACTTTTTGCTTGGCTTTTGCTCCGTCGCCATCGGGACTTTCTATAATCGGAAACATCCAGCAAGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAAATGTGCAAATTATATAATTAAGGGTTTGGAATATAGTTTTTCTTAATCACATTATTATTTTTGTTTTAAAGCTAATAGATAGATCACATGTAAATTATTTACTTCCCATTTAGTATATGCTATATACTTTCTATGAATGTTGTATTTTTCTTATTTTCAATCTAATAAAAACTACGATGGACGACCTGGTTT

Coding sequence (CDS)

ATGTTCTTTAAACAACTCTCTTCTCTTCTCTTCTTTCTTGCTGTCGTGGTCGCCGCCGTCGCCCGTCCGTCTTCTCCAACAAAGTTTCAAAACCTTAATGTTAAAGCTACCAAATTGGACTTCAATGACGGTCAGATTCTTCATACCCTTAATTTCTCCGACGGTCACCGGCAAGTTTCCGGTCATAAATCCCACAACAATACATTTAAACTCAACCTTCTCCATAGGGATAAACTGTCCCATGTTCATGGTCACCGTCATGGGTTTAATGACCGCATGAAAAGAGACGCCATAAGAGTAGCTACCCTCGTCCGCCGCCTATCCCACGGCGGCGCTCACGGAGCCGCCGCCGTGGAAGATAGCCGTTACAAGGTGGCTAATTTTGCCACGGATGTCATTTCAGGAATGGAAGAGGGGAGTGGAGAATATTTTGTTCGGATCGGAGTTGGGAGCCCACCGAGGAGTCAATATATGGTGATTGATTCCGGCAGTGATATTGTGTGGGTCCAATGTAAACCTTGTAGCCGATGCTACCAACAATCCGACCCGGTTTTCGACCCGGCCGACTCCTCCTCCTTCGCCGGCGTATCTTGTGGCTCCGCCGTTTGTGACCGCCTTGAGAACACCGGCTGTAACGCTGGACGATGTCGGTACGAAGTGTCCTATGGGGACGGGTCTTACACTAAAGGCACTCTCGCTCTTGAAACTCTCACCGTCGGCGAAGTGATGATTCGTGACGTGGCAATTGGGTGCGGTCATACAAACCAAGGAATGTTTATAGGCGCCGCCGGTTTACTCGGACTCGGTGGAGGGTCAATGTCCTTCATCGGCCAACTCGGTGGTCAAACCGGGGGAGCATTCAGCTATTGTTTAGTGAGTCGAGGCACCGGCTCAACCGGAACATTAGAATTCGGCCGTGGAGCATTGCCAGTTGGCGCCACGTGGATCTCCCTCATTCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCATCGGCGTCGGCGGAGCTCGAGTTTCAATACCAGAAGAAACGTTTCAACTCACCGAGTTCGGTACCAACGGCGTCGTAATGGACACTGGCACCGCCGTGACACGTCTTCCGACTGCGGCCTACGTGGCGTTCCGTGATTCGTTCACAGCCCAAACCAGCAACCTCCCACGCGCGGCTGGCGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAGTCCGTACGAGTGCCAACGGTGTCGTTTTACTTCTCTGATGGACCGGTTCTGACATTGCCAGCGAAGAACTTTTTGATTCCGGTAGACGGCGGCGGAACTTTTTGCTTGGCTTTTGCTCCGTCGCCATCGGGACTTTCTATAATCGGAAACATCCAGCAAGAAGGAATTCAAATTTCATTCGATGGGGCTAATGGGTTCGTTGGATTTGGCCCAAATATTTGCTAA

Protein sequence

MFFKQLSSLLFFLAVVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSDGHRQVSGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAHGAAAVEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC
Homology
BLAST of PI0007530 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 3.9e-180
Identity = 310/453 (68.43%), Postives = 367/453 (81.02%), Query Frame = 0

Query: 32  LNVKATKLDFNDGQILHTLNFSDGHRQVSGHKSHNNTFKLNLLHRDKLSHV--HGHRHGF 91
           L V AT  DFN+       +FSD           ++ + L LLHRD+   V    H H  
Sbjct: 37  LTVTATLPDFNN------THFSD---------ESSSKYTLRLLHRDRFPSVTYRNHHHRL 96

Query: 92  NDRMKRDAIRVATLVRRLSHGGAHGAAAVEDSRYKVANFATDVISGMEEGSGEYFVRIGV 151
           + RM+RD  RV+ ++RR+S           DSRY+V +F +D++SGM++GSGEYFVRIGV
Sbjct: 97  HARMRRDTDRVSAILRRIS----GKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGV 156

Query: 152 GSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENT 211
           GSPPR QYMVIDSGSD+VWVQC+PC  CY+QSDPVFDPA S S+ GVSCGS+VCDR+EN+
Sbjct: 157 GSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENS 216

Query: 212 GCNAGRCRYEVSYGDGSYTKGTLALETLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLG 271
           GC++G CRYEV YGDGSYTKGTLALETLT  + ++R+VA+GCGH N+GMFIGAAGLLG+G
Sbjct: 217 GCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIG 276

Query: 272 GGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATWISLIRNPRAPSFYY 331
           GGSMSF+GQL GQTGGAF YCLVSRGT STG+L FGR ALPVGA+W+ L+RNPRAPSFYY
Sbjct: 277 GGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYY 336

Query: 332 IGLAGIGVGGARVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNL 391
           +GL G+GVGG R+ +P+  F LTE G  GVVMDTGTAVTRLPTAAYVAFRD F +QT+NL
Sbjct: 337 VGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANL 396

Query: 392 PRAAGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPS 451
           PRA+GVSIFDTCYDL+GF SVRVPTVSFYF++GPVLTLPA+NFL+PVD  GT+C AFA S
Sbjct: 397 PRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAAS 456

Query: 452 PSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
           P+GLSIIGNIQQEGIQ+SFDGANGFVGFGPN+C
Sbjct: 457 PTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of PI0007530 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 7.6e-107
Identity = 191/399 (47.87%), Postives = 270/399 (67.67%), Query Frame = 0

Query: 92  RMKRDAIRVATLVRRLSHG--GAHGA----AAVEDSRYKVANFATDVISGMEEGSGEYFV 151
           R++RD+ RVA +V ++     G   +       ED+RY+  +  T V+SG  +GSGEYF 
Sbjct: 105 RLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFS 164

Query: 152 RIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSAVCDR 211
           RIGVG+P +  Y+V+D+GSD+ W+QC+PC+ CYQQSDPVF+P  SS++  ++C +  C  
Sbjct: 165 RIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSL 224

Query: 212 LENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGEV-MIRDVAIGCGHTNQGMFIGAAG 271
           LE + C + +C Y+VSYGDGS+T G LA +T+T G    I +VA+GCGH N+G+F GAAG
Sbjct: 225 LETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAG 284

Query: 272 LLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATWISLIRNPRA 331
           LLGLGGG +S   Q+      +FSYCLV R +G + +L+F    L  G     L+RN + 
Sbjct: 285 LLGLGGGVLSITNQM---KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKI 344

Query: 332 PSFYYIGLAGIGVGGARVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTA 391
            +FYY+GL+G  VGG +V +P+  F +   G+ GV++D GTAVTRL T AY + RD+F  
Sbjct: 345 DTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLK 404

Query: 392 QTSNLPR-AAGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFC 451
            T NL + ++ +S+FDTCYD +   +V+VPTV+F+F+ G  L LPAKN+LIPVD  GTFC
Sbjct: 405 LTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFC 464

Query: 452 LAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
            AFAP+ S LSIIGN+QQ+G +I++D +   +G   N C
Sbjct: 465 FAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of PI0007530 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.2e-104
Identity = 219/489 (44.79%), Postives = 295/489 (60.33%), Query Frame = 0

Query: 6   LSSLLFFLAVVVAAVARPSSPTKFQNLNVK--ATKLDF---NDGQILHTLNFSDGHRQVS 65
           L SL FF   + +  + PS  T F N +    A+ + F   +D + L    F  G    S
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSES 68

Query: 66  GHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRV---ATLVRRLSHGGAHGAAA 125
                +++  LNL H D LS        F+ R++RD+ RV   ATL  ++       A  
Sbjct: 69  -----SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP- 128

Query: 126 VEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 185
                 +   F++ V+SG+ +GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC RC
Sbjct: 129 ------RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC 188

Query: 186 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGR--CRYEVSYGDGSYTKGTLALE 245
           Y QSDP+FDP  S ++A + C S  C RL++ GCN  R  C Y+VSYGDGS+T G  + E
Sbjct: 189 YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTE 248

Query: 246 TLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRG 305
           TLT     ++ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R 
Sbjct: 249 TLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRS 308

Query: 306 TGS-TGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARV-SIPEETFQLTE 365
             S   ++ FG  A+   A +  L+ NP+  +FYY+GL GI VGG RV  +    F+L +
Sbjct: 309 ASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQ 368

Query: 366 FGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVP 425
            G  GV++D+GT+VTRL   AY+A RD+F      L RA   S+FDTC+DL+    V+VP
Sbjct: 369 IGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVP 428

Query: 426 TVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANG 483
           TV  +F  G  ++LPA N+LIPVD  G FC AFA +  GLSIIGNIQQ+G ++ +D A+ 
Sbjct: 429 TVVLHFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASS 484

BLAST of PI0007530 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.4e-70
Identity = 150/357 (42.02%), Postives = 202/357 (56.58%), Query Frame = 0

Query: 135 GMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSR-CYQQSDPVFDPADSSSF 194
           G   GSG Y V +G+G+P     ++ D+GSD+ W QC+PC R CY Q +P+F+P+ S+S+
Sbjct: 124 GSTLGSGNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSY 183

Query: 195 AGVSCGSAVCDRLENT-----GCNAGRCRYEVSYGDGSYTKGTLALETLTVGEVMIRD-V 254
             VSC SA C  L +       C+A  C Y + YGD S++ G LA E  T+    + D V
Sbjct: 184 YNVSCSSAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGV 243

Query: 255 AIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRG 314
             GCG  NQG+F G AGLLGLG   +SF  Q        FSYCL S     TG L FG  
Sbjct: 244 YFGCGENNQGLFTGVAGLLGLGRDKLSFPSQTATAYNKIFSYCLPS-SASYTGHLTFGSA 303

Query: 315 ALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTNGVVMDTGTAV 374
            +     +  +       SFY + +  I VGG ++ IP      T F T G ++D+GT +
Sbjct: 304 GISRSVKFTPISTITDGTSFYGLNIVAITVGGQKLPIPS-----TVFSTPGALIDSGTVI 363

Query: 375 TRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTL 434
           TRLP  AY A R SF A+ S  P  +GVSI DTC+DL+GF++V +P V+F FS G V+ L
Sbjct: 364 TRLPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVEL 423

Query: 435 PAKNFLIPVDGGGTFCLAFA--PSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
            +K  +  V      CLAFA     S  +I GN+QQ+ +++ +DGA G VGF PN C
Sbjct: 424 GSKG-IFYVFKISQVCLAFAGNSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

BLAST of PI0007530 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.3e-68
Identity = 158/429 (36.83%), Postives = 226/429 (52.68%), Query Frame = 0

Query: 59  VSGHKSHNNTFKLNLLH-RDKLSHVHGH-RHGFNDRMKRDAIRVATLVRRLSHGGAHGAA 118
           V   K+ N    L ++H     SH+    R   ++ ++RD  RV ++  +LS   A+  +
Sbjct: 53  VPSSKASNTKSSLRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSKNSANEVS 112

Query: 119 AVEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPC-S 178
             + +            SG+  GSG Y V IG+G+P     +V D+GSD+ W QC+PC  
Sbjct: 113 EAKSTELPAK-------SGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLG 172

Query: 179 RCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALE 238
            CY Q +P F+P+ SS++  VSC S +C+  E+  C+A  C Y + YGD S+T+G LA E
Sbjct: 173 SCYSQKEPKFNPSSSSTYQNVSCSSPMCEDAES--CSASNCVYSIVYGDKSFTQGFLAKE 232

Query: 239 --TLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVS 298
             TLT  +V + DV  GCG  NQG+F G AGLLGLG G +S   Q        FSYCL S
Sbjct: 233 KFTLTNSDV-LEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPS 292

Query: 299 RGTGSTGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTE 358
             + STG L FG   +     +  +   P A + Y I + GI VG   ++I   +     
Sbjct: 293 FTSNSTGHLTFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNS----- 352

Query: 359 FGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVP 418
           F T G ++D+GT  TRLPT  Y   R  F  + S+    +G  +FDTCYD  G ++V  P
Sbjct: 353 FSTEGAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYP 412

Query: 419 TVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANG 478
           T++F F+   V+ L      +P+      CLAFA +    +I GN+QQ  + + +D A G
Sbjct: 413 TIAFSFAGSTVVELDGSGISLPIK-ISQVCLAFAGNDDLPAIFGNVQQTTLDVVYDVAGG 464

Query: 479 FVGFGPNIC 483
            VGF PN C
Sbjct: 473 RVGFAPNGC 464

BLAST of PI0007530 vs. ExPASy TrEMBL
Match: A0A1S3BEG7 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103488783 PE=4 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.3e-263
Identity = 465/485 (95.88%), Postives = 469/485 (96.70%), Query Frame = 0

Query: 1   MFFKQL-SSLLFFLA--VVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSDGHR 60
           MFFKQL SSLLFFLA  VVVAA ARPSSPTKFQ LNVKATKLDFNDGQILHTLNFSD HR
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  QVSGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAHGAAA 120
           QVSGHKSHN+TFKLNLLHRDKLSHVHGHR GFNDRMKRDAIRVATLVRRLS    HGAAA
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLS----HGAAA 120

Query: 121 VEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180
           VEDSR+KVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC
Sbjct: 121 VEDSRFKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180

Query: 181 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240
           YQQSDPVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL
Sbjct: 181 YQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240

Query: 241 TVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300
           TVG+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG
Sbjct: 241 TVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300

Query: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTN 360
           STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGG RVSIPEETFQLTEFGTN
Sbjct: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTN 360

Query: 361 GVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSF 420
           GVVMDTGTAVTRLPT+AYVA RDSFTAQTSNLPRA GVSIFDTCYDLNGFESVRVPTVSF
Sbjct: 361 GVVMDTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 420

Query: 421 YFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480
           YFSDGP LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF
Sbjct: 421 YFSDGPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480

Query: 481 GPNIC 483
           GPNIC
Sbjct: 481 GPNIC 481

BLAST of PI0007530 vs. ExPASy TrEMBL
Match: A0A0A0KPR4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579060 PE=4 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.1e-257
Identity = 454/485 (93.61%), Postives = 462/485 (95.26%), Query Frame = 0

Query: 1   MFFKQL-SSLLFFLAVVV--AAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSDGHR 60
           MFFKQL SSLLF L V+V  AA AR SS TKFQ LNVKATKLDFNDGQILH LNFSDGHR
Sbjct: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60

Query: 61  QVSGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAHGAAA 120
           QVSG+KS NNTFKLNLLHRDKLSHVHGHR GFNDRMKRDAIRVATLVRRLSHG     AA
Sbjct: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHG---APAA 120

Query: 121 VEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180
           V+DSRYKVANFATDVISGME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQCKPCSRC
Sbjct: 121 VKDSRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRC 180

Query: 181 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240
           YQQSDPVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL
Sbjct: 181 YQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240

Query: 241 TVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300
           TVG+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG
Sbjct: 241 TVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300

Query: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTN 360
           STG LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGG RVS+PEETFQLTE+GTN
Sbjct: 301 STGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTN 360

Query: 361 GVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSF 420
           GVVMDTGTAVTR PTAAYVAFRDSFTAQTSNLPRA GVSIFDTCYDLNGFESVRVPTVSF
Sbjct: 361 GVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 420

Query: 421 YFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480
           YFSDGPVLTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF
Sbjct: 421 YFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480

Query: 481 GPNIC 483
           GPNIC
Sbjct: 481 GPNIC 482

BLAST of PI0007530 vs. ExPASy TrEMBL
Match: A0A5A7SVY2 (Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00740 PE=4 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.4e-252
Identity = 451/485 (92.99%), Postives = 455/485 (93.81%), Query Frame = 0

Query: 1   MFFKQL-SSLLFFLA--VVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSDGHR 60
           MFFKQL SSLLFFLA  VVVAA ARPSSPTKFQ LNVKATKLDFNDGQILHTLNFSD HR
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  QVSGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAHGAAA 120
           QVSGHKSHN+TFKLNLLHRDKLSHVHGHR GFNDRMKRDAIRVATLVRRLS    HGAAA
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLS----HGAAA 120

Query: 121 VEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180
           VEDSR+K              GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC
Sbjct: 121 VEDSRFK--------------GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180

Query: 181 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240
           YQQSDPVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL
Sbjct: 181 YQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240

Query: 241 TVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300
           TVG+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG
Sbjct: 241 TVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300

Query: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTN 360
           STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGG RVSIPEETFQLTEFGTN
Sbjct: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTN 360

Query: 361 GVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSF 420
           GVVMDTGTAVTRLPT+AYVA RDSFTAQTSNLPRA GVSIFDTCYDLNGFESVRVPTVSF
Sbjct: 361 GVVMDTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 420

Query: 421 YFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480
           YFSDGP LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF
Sbjct: 421 YFSDGPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 467

Query: 481 GPNIC 483
           GPNIC
Sbjct: 481 GPNIC 467

BLAST of PI0007530 vs. ExPASy TrEMBL
Match: A0A6J1KA06 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493608 PE=4 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 1.3e-231
Identity = 409/490 (83.47%), Postives = 438/490 (89.39%), Query Frame = 0

Query: 1   MFFKQLSS------LLFFLAVVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSD 60
           MF K  SS       L FLA   AA+ARPS P  F  LNVK TKL+ N  QIL TLNFSD
Sbjct: 1   MFLKHPSSPLLLLLHLLFLAAAAAALARPSLPPNFLYLNVKPTKLELNHTQILPTLNFSD 60

Query: 61  GHRQVSGHKSHNNTFKLNLLHRDKLSHVHG--HRHGFNDRMKRDAIRVATLVRRLSHGGA 120
            H QV+  K HN T KLNLLHRDKL HVH   HRH FN+R+KRDAIRVATLVRRLS    
Sbjct: 61  AHLQVTAQKFHNTTLKLNLLHRDKLPHVHAHRHRHAFNERIKRDAIRVATLVRRLS---- 120

Query: 121 HGAAAVEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCK 180
           H AAAV+D+ YKVANFAT V+SGMEEGSGE+FVR+GVGSPPRSQYMVIDSGSDIVWVQC+
Sbjct: 121 HTAAAVQDNSYKVANFATGVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWVQCQ 180

Query: 181 PCSRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTL 240
           PC++CYQQSDPVF+PADSSSFAGVSCGSAVC RL+NT C+AGRCRYEVSYGDGSYTKGTL
Sbjct: 181 PCTQCYQQSDPVFNPADSSSFAGVSCGSAVCGRLDNTDCHAGRCRYEVSYGDGSYTKGTL 240

Query: 241 ALETLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLV 300
           ALET+T+G+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLV
Sbjct: 241 ALETITMGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLV 300

Query: 301 SRGTGSTGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLT 360
           SR TGSTGTLEFGRGA+PVGATWISLIRNPRAPSFYYIGLAG+GVGG RV IPEETFQL+
Sbjct: 301 SRDTGSTGTLEFGRGAMPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETFQLS 360

Query: 361 EFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRV 420
           E+GTNGVVMDTGTAVTRLPTAAY AFRD+FTAQT+NLPRA GVSIFDTC+DLNGFES+RV
Sbjct: 361 EYGTNGVVMDTGTAVTRLPTAAYEAFRDAFTAQTNNLPRAGGVSIFDTCFDLNGFESIRV 420

Query: 421 PTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGAN 480
           PTVSFYFSDGPVLTLPAKNFLIPV+G GTFCLAFAPSPSGLSIIGNIQQEGIQIS DGAN
Sbjct: 421 PTVSFYFSDGPVLTLPAKNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISIDGAN 480

Query: 481 GFVGFGPNIC 483
           GFVGFGPN+C
Sbjct: 481 GFVGFGPNVC 486

BLAST of PI0007530 vs. ExPASy TrEMBL
Match: A0A6J1GXH5 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458389 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.1e-229
Identity = 405/493 (82.15%), Postives = 435/493 (88.24%), Query Frame = 0

Query: 1   MFFKQLSS-------LLFFLAVVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFS 60
           MF K  SS       LL FLA   AA+ARPS P  F  LNVK TKL+ N   IL TLNFS
Sbjct: 1   MFLKHPSSPLLLLLHLLLFLAAASAALARPSPPPNFLYLNVKPTKLELNHTHILPTLNFS 60

Query: 61  DGHRQVSGHKSHNNTFKLNLLHRDKLSHVHG----HRHGFNDRMKRDAIRVATLVRRLSH 120
             H QV+  K HN T KLNLLHRDKL HVH     HRH FN+R+KRDAIRVATLVRRLS 
Sbjct: 61  HAHLQVTAQKFHNTTLKLNLLHRDKLPHVHALRHRHRHAFNERIKRDAIRVATLVRRLS- 120

Query: 121 GGAHGAAAVEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWV 180
              H AAAV+D+ YKVA+FATDV+SGMEEGSGE+FVR+GVGSPPRSQYMVIDSGSDIVWV
Sbjct: 121 ---HAAAAVQDNSYKVASFATDVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWV 180

Query: 181 QCKPCSRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTK 240
           QC+PC++CYQQSDPVF+PADSSSF GVSCGS VCDRL+NT C+AGRCRYEVSYGDGSYTK
Sbjct: 181 QCQPCTQCYQQSDPVFNPADSSSFEGVSCGSTVCDRLDNTDCHAGRCRYEVSYGDGSYTK 240

Query: 241 GTLALETLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSY 300
           GTLALET+T+G+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMS IGQLGGQTGGAFSY
Sbjct: 241 GTLALETITMGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSLIGQLGGQTGGAFSY 300

Query: 301 CLVSRGTGSTGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETF 360
           CLVSR TGSTGTLEFGRGA+PVGATWISLIRNPRAPSFYYIGLAG+GVGG RV IPEETF
Sbjct: 301 CLVSRDTGSTGTLEFGRGAMPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETF 360

Query: 361 QLTEFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFES 420
           QL+E+GTNGVVMDTGTAVTRLPTAAY AFRD+F AQT+NLPRA GVSIFDTC+DLNGFES
Sbjct: 361 QLSEYGTNGVVMDTGTAVTRLPTAAYEAFRDAFIAQTNNLPRAGGVSIFDTCFDLNGFES 420

Query: 421 VRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFD 480
           +RVPTVSFYFSDGPVLTLPAKNFLIPV+G GTFCLAFAPSPSGLSIIGNIQQEGIQIS D
Sbjct: 421 IRVPTVSFYFSDGPVLTLPAKNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISID 480

Query: 481 GANGFVGFGPNIC 483
           GANGFVGFGPN+C
Sbjct: 481 GANGFVGFGPNVC 489

BLAST of PI0007530 vs. NCBI nr
Match: XP_008445900.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo])

HSP 1 Score: 917.5 bits (2370), Expect = 4.7e-263
Identity = 465/485 (95.88%), Postives = 469/485 (96.70%), Query Frame = 0

Query: 1   MFFKQL-SSLLFFLA--VVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSDGHR 60
           MFFKQL SSLLFFLA  VVVAA ARPSSPTKFQ LNVKATKLDFNDGQILHTLNFSD HR
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  QVSGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAHGAAA 120
           QVSGHKSHN+TFKLNLLHRDKLSHVHGHR GFNDRMKRDAIRVATLVRRLS    HGAAA
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLS----HGAAA 120

Query: 121 VEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180
           VEDSR+KVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC
Sbjct: 121 VEDSRFKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180

Query: 181 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240
           YQQSDPVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL
Sbjct: 181 YQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240

Query: 241 TVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300
           TVG+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG
Sbjct: 241 TVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300

Query: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTN 360
           STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGG RVSIPEETFQLTEFGTN
Sbjct: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTN 360

Query: 361 GVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSF 420
           GVVMDTGTAVTRLPT+AYVA RDSFTAQTSNLPRA GVSIFDTCYDLNGFESVRVPTVSF
Sbjct: 361 GVVMDTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 420

Query: 421 YFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480
           YFSDGP LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF
Sbjct: 421 YFSDGPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480

Query: 481 GPNIC 483
           GPNIC
Sbjct: 481 GPNIC 481

BLAST of PI0007530 vs. NCBI nr
Match: XP_004147103.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothetical protein Csa_008597 [Cucumis sativus])

HSP 1 Score: 896.7 bits (2316), Expect = 8.5e-257
Identity = 454/485 (93.61%), Postives = 462/485 (95.26%), Query Frame = 0

Query: 1   MFFKQL-SSLLFFLAVVV--AAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSDGHR 60
           MFFKQL SSLLF L V+V  AA AR SS TKFQ LNVKATKLDFNDGQILH LNFSDGHR
Sbjct: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60

Query: 61  QVSGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAHGAAA 120
           QVSG+KS NNTFKLNLLHRDKLSHVHGHR GFNDRMKRDAIRVATLVRRLSHG     AA
Sbjct: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHG---APAA 120

Query: 121 VEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180
           V+DSRYKVANFATDVISGME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQCKPCSRC
Sbjct: 121 VKDSRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRC 180

Query: 181 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240
           YQQSDPVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL
Sbjct: 181 YQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240

Query: 241 TVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300
           TVG+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG
Sbjct: 241 TVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300

Query: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTN 360
           STG LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGG RVS+PEETFQLTE+GTN
Sbjct: 301 STGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTN 360

Query: 361 GVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSF 420
           GVVMDTGTAVTR PTAAYVAFRDSFTAQTSNLPRA GVSIFDTCYDLNGFESVRVPTVSF
Sbjct: 361 GVVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 420

Query: 421 YFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480
           YFSDGPVLTLPA+NFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF
Sbjct: 421 YFSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480

Query: 481 GPNIC 483
           GPNIC
Sbjct: 481 GPNIC 482

BLAST of PI0007530 vs. NCBI nr
Match: XP_038893071.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida])

HSP 1 Score: 887.9 bits (2293), Expect = 4.0e-254
Identity = 447/483 (92.55%), Postives = 458/483 (94.82%), Query Frame = 0

Query: 1   MFFKQLSSLLFFL-AVVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSDGHRQV 60
           MF KQ SSLLFFL  +  +A A  SSPTKFQ LNVKATKLDFND QILHTLNFS   RQV
Sbjct: 1   MFLKQPSSLLFFLFFLFFSAAAGRSSPTKFQYLNVKATKLDFNDDQILHTLNFSHARRQV 60

Query: 61  SGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAHGAAAVE 120
           SGHKS NNTFKLNLLHRDKLSHVHGHRHGFN+R+KRDAIRVATLVRRLSHGG     AV+
Sbjct: 61  SGHKSDNNTFKLNLLHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGG----GAVQ 120

Query: 121 DSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQ 180
           D++YKVA+FATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQ
Sbjct: 121 DNKYKVADFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQ 180

Query: 181 QSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTV 240
           QSDPVFDPADSSSFAGVSC S VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTV
Sbjct: 181 QSDPVFDPADSSSFAGVSCDSIVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTV 240

Query: 241 GEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGST 300
           GEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGST
Sbjct: 241 GEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGST 300

Query: 301 GTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTNGV 360
           GTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGG RVS+PEETFQLTE+GTNGV
Sbjct: 301 GTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGV 360

Query: 361 VMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSFYF 420
           VMDTGTAVTRLPTAAYVA RDSFTAQTSNLPRA GVSIFDTCYDLNGFESVRVPTVSFYF
Sbjct: 361 VMDTGTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYF 420

Query: 421 SDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 480
           SDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP
Sbjct: 421 SDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 479

Query: 481 NIC 483
           N+C
Sbjct: 481 NVC 479

BLAST of PI0007530 vs. NCBI nr
Match: KAA0034086.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 881.7 bits (2277), Expect = 2.8e-252
Identity = 451/485 (92.99%), Postives = 455/485 (93.81%), Query Frame = 0

Query: 1   MFFKQL-SSLLFFLA--VVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFSDGHR 60
           MFFKQL SSLLFFLA  VVVAA ARPSSPTKFQ LNVKATKLDFNDGQILHTLNFSD HR
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  QVSGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAHGAAA 120
           QVSGHKSHN+TFKLNLLHRDKLSHVHGHR GFNDRMKRDAIRVATLVRRLS    HGAAA
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLS----HGAAA 120

Query: 121 VEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180
           VEDSR+K              GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC
Sbjct: 121 VEDSRFK--------------GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 180

Query: 181 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240
           YQQSDPVFDPADSSSFAGVSCGS VCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL
Sbjct: 181 YQQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETL 240

Query: 241 TVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300
           TVG+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG
Sbjct: 241 TVGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTG 300

Query: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTEFGTN 360
           STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGG RVSIPEETFQLTEFGTN
Sbjct: 301 STGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTN 360

Query: 361 GVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSF 420
           GVVMDTGTAVTRLPT+AYVA RDSFTAQTSNLPRA GVSIFDTCYDLNGFESVRVPTVSF
Sbjct: 361 GVVMDTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 420

Query: 421 YFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 480
           YFSDGP LTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF
Sbjct: 421 YFSDGPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGF 467

Query: 481 GPNIC 483
           GPNIC
Sbjct: 481 GPNIC 467

BLAST of PI0007530 vs. NCBI nr
Match: XP_023547721.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 817.8 bits (2111), Expect = 5.0e-233
Identity = 409/489 (83.64%), Postives = 439/489 (89.78%), Query Frame = 0

Query: 1   MFFKQLSS-------LLFFLAVVVAAVARPSSPTKFQNLNVKATKLDFNDGQILHTLNFS 60
           MF K  SS       LL FLA   AA+ARPS P  F  LNVK TKL+ N   IL TLNFS
Sbjct: 1   MFLKHPSSPLLLLLHLLLFLA-AAAALARPSPPPNFLYLNVKPTKLELNHTHILPTLNFS 60

Query: 61  DGHRQVSGHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRVATLVRRLSHGGAH 120
           D H QV+  K HN T KLNLLHRDKL HVH HRH FN+R+KRDAIRVATL+RRLSH    
Sbjct: 61  DAHLQVTAQKFHNTTLKLNLLHRDKLPHVHAHRHAFNERIKRDAIRVATLIRRLSHA--- 120

Query: 121 GAAAVEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKP 180
            AAA +D+ YKVANFATDV+SGMEEGSGE+FVR+GVGSPPRSQYMVIDSGSDIVWVQC+P
Sbjct: 121 AAAAAQDNSYKVANFATDVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWVQCQP 180

Query: 181 CSRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLA 240
           C++CYQQSDPVF+PADSSSFAGVSCGSAVCDRL+NT C+AGRCRYEVSYGDGSYTKGTLA
Sbjct: 181 CTQCYQQSDPVFNPADSSSFAGVSCGSAVCDRLDNTDCHAGRCRYEVSYGDGSYTKGTLA 240

Query: 241 LETLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVS 300
           LET+T+G+VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVS
Sbjct: 241 LETITMGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVS 300

Query: 301 RGTGSTGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARVSIPEETFQLTE 360
           R TGSTGTLEFGRGA+PVGATWISLIRNPRAPSFYYIGLAG+GVGG RV IPEETFQL+E
Sbjct: 301 RDTGSTGTLEFGRGAMPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETFQLSE 360

Query: 361 FGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVP 420
           +GTNGVVMDTGTAVTRLPTAAY AFRD+FTAQT+NLPRA GVSIFDTC+DLNGFES+RVP
Sbjct: 361 YGTNGVVMDTGTAVTRLPTAAYEAFRDAFTAQTNNLPRAGGVSIFDTCFDLNGFESIRVP 420

Query: 421 TVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANG 480
           TVSFYFSDGPVLTLPAKNFLIPV+G GTFCLAFAPSPSGLSIIGNIQQEGIQIS DGANG
Sbjct: 421 TVSFYFSDGPVLTLPAKNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISIDGANG 480

Query: 481 FVGFGPNIC 483
           FVGFGPN+C
Sbjct: 481 FVGFGPNVC 485

BLAST of PI0007530 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 632.5 bits (1630), Expect = 2.8e-181
Identity = 310/453 (68.43%), Postives = 367/453 (81.02%), Query Frame = 0

Query: 32  LNVKATKLDFNDGQILHTLNFSDGHRQVSGHKSHNNTFKLNLLHRDKLSHV--HGHRHGF 91
           L V AT  DFN+       +FSD           ++ + L LLHRD+   V    H H  
Sbjct: 37  LTVTATLPDFNN------THFSD---------ESSSKYTLRLLHRDRFPSVTYRNHHHRL 96

Query: 92  NDRMKRDAIRVATLVRRLSHGGAHGAAAVEDSRYKVANFATDVISGMEEGSGEYFVRIGV 151
           + RM+RD  RV+ ++RR+S           DSRY+V +F +D++SGM++GSGEYFVRIGV
Sbjct: 97  HARMRRDTDRVSAILRRIS----GKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGV 156

Query: 152 GSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSAVCDRLENT 211
           GSPPR QYMVIDSGSD+VWVQC+PC  CY+QSDPVFDPA S S+ GVSCGS+VCDR+EN+
Sbjct: 157 GSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENS 216

Query: 212 GCNAGRCRYEVSYGDGSYTKGTLALETLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLG 271
           GC++G CRYEV YGDGSYTKGTLALETLT  + ++R+VA+GCGH N+GMFIGAAGLLG+G
Sbjct: 217 GCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIG 276

Query: 272 GGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATWISLIRNPRAPSFYY 331
           GGSMSF+GQL GQTGGAF YCLVSRGT STG+L FGR ALPVGA+W+ L+RNPRAPSFYY
Sbjct: 277 GGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYY 336

Query: 332 IGLAGIGVGGARVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNL 391
           +GL G+GVGG R+ +P+  F LTE G  GVVMDTGTAVTRLPTAAYVAFRD F +QT+NL
Sbjct: 337 VGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANL 396

Query: 392 PRAAGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPS 451
           PRA+GVSIFDTCYDL+GF SVRVPTVSFYF++GPVLTLPA+NFL+PVD  GT+C AFA S
Sbjct: 397 PRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAAS 456

Query: 452 PSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
           P+GLSIIGNIQQEGIQ+SFDGANGFVGFGPN+C
Sbjct: 457 PTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of PI0007530 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 396.4 bits (1017), Expect = 3.4e-110
Identity = 195/398 (48.99%), Postives = 272/398 (68.34%), Query Frame = 0

Query: 92  RMKRDAIRVATLVRRLSHGGAHGAAAVEDSRYKVANFATD-------VISGMEEGSGEYF 151
           R+ RD  RV +L+ RL    A    +  D +     + T+       +ISG  +GSGEYF
Sbjct: 92  RLNRDTARVKSLITRLDL--AINNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYF 151

Query: 152 VRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSAVCD 211
            R+G+G P R  YMV+D+GSD+ W+QC PC+ CY Q++P+F+P+ SSS+  +SC +  C+
Sbjct: 152 TRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCN 211

Query: 212 RLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGEVMIRDVAIGCGHTNQGMFIGAAG 271
            LE + C    C YEVSYGDGSYT G  A ETLT+G  ++++VA+GCGH+N+G+F+GAAG
Sbjct: 212 ALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCGHSNEGLFVGAAG 271

Query: 272 LLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATWISLIRNPRA 331
           LLGLGGG ++   QL      +FSYCLV R + S  T++FG    P  A    L+RN + 
Sbjct: 272 LLGLGGGLLALPSQLNTT---SFSYCLVDRDSDSASTVDFGTSLSP-DAVVAPLLRNHQL 331

Query: 332 PSFYYIGLAGIGVGGARVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTA 391
            +FYY+GL GI VGG  + IP+ +F++ E G+ G+++D+GTAVTRL T  Y + RDSF  
Sbjct: 332 DTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVK 391

Query: 392 QTSNLPRAAGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCL 451
            T +L +AAGV++FDTCY+L+   +V VPTV+F+F  G +L LPAKN++IPVD  GTFCL
Sbjct: 392 GTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCL 451

Query: 452 AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
           AFAP+ S L+IIGN+QQ+G +++FD AN  +GF  N C
Sbjct: 452 AFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

BLAST of PI0007530 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 389.0 bits (998), Expect = 5.4e-108
Identity = 191/399 (47.87%), Postives = 270/399 (67.67%), Query Frame = 0

Query: 92  RMKRDAIRVATLVRRLSHG--GAHGA----AAVEDSRYKVANFATDVISGMEEGSGEYFV 151
           R++RD+ RVA +V ++     G   +       ED+RY+  +  T V+SG  +GSGEYF 
Sbjct: 105 RLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFS 164

Query: 152 RIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAGVSCGSAVCDR 211
           RIGVG+P +  Y+V+D+GSD+ W+QC+PC+ CYQQSDPVF+P  SS++  ++C +  C  
Sbjct: 165 RIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSL 224

Query: 212 LENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGEV-MIRDVAIGCGHTNQGMFIGAAG 271
           LE + C + +C Y+VSYGDGS+T G LA +T+T G    I +VA+GCGH N+G+F GAAG
Sbjct: 225 LETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAG 284

Query: 272 LLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATWISLIRNPRA 331
           LLGLGGG +S   Q+      +FSYCLV R +G + +L+F    L  G     L+RN + 
Sbjct: 285 LLGLGGGVLSITNQM---KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKI 344

Query: 332 PSFYYIGLAGIGVGGARVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTA 391
            +FYY+GL+G  VGG +V +P+  F +   G+ GV++D GTAVTRL T AY + RD+F  
Sbjct: 345 DTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLK 404

Query: 392 QTSNLPR-AAGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFC 451
            T NL + ++ +S+FDTCYD +   +V+VPTV+F+F+ G  L LPAKN+LIPVD  GTFC
Sbjct: 405 LTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFC 464

Query: 452 LAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 483
            AFAP+ S LSIIGN+QQ+G +I++D +   +G   N C
Sbjct: 465 FAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of PI0007530 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 381.7 bits (979), Expect = 8.6e-106
Identity = 219/489 (44.79%), Postives = 295/489 (60.33%), Query Frame = 0

Query: 6   LSSLLFFLAVVVAAVARPSSPTKFQNLNVK--ATKLDF---NDGQILHTLNFSDGHRQVS 65
           L SL FF   + +  + PS  T F N +    A+ + F   +D + L    F  G    S
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSES 68

Query: 66  GHKSHNNTFKLNLLHRDKLSHVHGHRHGFNDRMKRDAIRV---ATLVRRLSHGGAHGAAA 125
                +++  LNL H D LS        F+ R++RD+ RV   ATL  ++       A  
Sbjct: 69  -----SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP- 128

Query: 126 VEDSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRC 185
                 +   F++ V+SG+ +GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC RC
Sbjct: 129 ------RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC 188

Query: 186 YQQSDPVFDPADSSSFAGVSCGSAVCDRLENTGCNAGR--CRYEVSYGDGSYTKGTLALE 245
           Y QSDP+FDP  S ++A + C S  C RL++ GCN  R  C Y+VSYGDGS+T G  + E
Sbjct: 189 YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTE 248

Query: 246 TLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRG 305
           TLT     ++ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R 
Sbjct: 249 TLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRS 308

Query: 306 TGS-TGTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARV-SIPEETFQLTE 365
             S   ++ FG  A+   A +  L+ NP+  +FYY+GL GI VGG RV  +    F+L +
Sbjct: 309 ASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQ 368

Query: 366 FGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFESVRVP 425
            G  GV++D+GT+VTRL   AY+A RD+F      L RA   S+FDTC+DL+    V+VP
Sbjct: 369 IGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVP 428

Query: 426 TVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANG 483
           TV  +F  G  ++LPA N+LIPVD  G FC AFA +  GLSIIGNIQQ+G ++ +D A+ 
Sbjct: 429 TVVLHFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASS 484

BLAST of PI0007530 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 360.5 bits (924), Expect = 2.1e-99
Identity = 210/493 (42.60%), Postives = 295/493 (59.84%), Query Frame = 0

Query: 4   KQLSSLLFFLAVVVAAVARPSS--PTKFQNLNVKATKLDFNDGQILHTLNFSDGHRQVSG 63
           K L++L F +  V+   +  SS   T   N    +  L + + + L   + S+    +S 
Sbjct: 4   KVLNTLAFSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTSLSV 63

Query: 64  HKSHNNTFKLNLLHRDKLSHVHGHRHG--FNDRMKRDAIRVATLVRRLSHGGAHGAAAVE 123
           H          L H D LS          FN R++RD++RV ++    +   + G  A +
Sbjct: 64  H----------LSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAA--VSTGRNATK 123

Query: 124 DSRYKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQ 183
            +      F+  VISG+ +GSGEYF+R+GVG+P  + YMV+D+GSD+VW+QC PC  CY 
Sbjct: 124 RTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYN 183

Query: 184 QSDPVFDPADSSSFAGVSCGSAVCDRLENTG-CNAGR---CRYEVSYGDGSYTKGTLALE 243
           Q+D +FDP  S +FA V CGS +C RL+++  C   R   C Y+VSYGDGS+T+G  + E
Sbjct: 184 QTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTE 243

Query: 244 TLTVGEVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSR- 303
           TLT     +  V +GCGH N+G+F+GAAGLLGLG G +SF  Q   +  G FSYCLV R 
Sbjct: 244 TLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRT 303

Query: 304 GTGST----GTLEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGARV-SIPEETF 363
            +GS+     T+ FG  A+P  + +  L+ NP+  +FYY+ L GI VGG+RV  + E  F
Sbjct: 304 SSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQF 363

Query: 364 QLTEFGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAAGVSIFDTCYDLNGFES 423
           +L   G  GV++D+GT+VTRL   AYVA RD+F    + L RA   S+FDTC+DL+G  +
Sbjct: 364 KLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTT 423

Query: 424 VRVPTVSFYFSDGPVLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFD 483
           V+VPTV F+F  G V +LPA N+LIPV+  G FC AFA +   LSIIGNIQQ+G ++++D
Sbjct: 424 VKVPTVVFHFGGGEV-SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYD 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHE33.9e-18068.43Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LS407.6e-10747.87Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ31.2e-10444.79Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q8S9J64.4e-7042.02Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LEW35.3e-6836.83Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BEG72.3e-26395.88protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KPR44.1e-25793.61Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G57906... [more]
A0A5A7SVY21.4e-25292.99Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1KA061.3e-23183.47protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GXH52.1e-22982.15protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
XP_008445900.14.7e-26395.88PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo][more]
XP_004147103.18.5e-25793.61protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KGN51568.1 hypothet... [more]
XP_038893071.14.0e-25492.55protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida][more]
KAA0034086.12.8e-25292.99protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa][more]
XP_023547721.15.0e-23383.64protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G20015.12.8e-18168.43Eukaryotic aspartyl protease family protein [more]
AT1G25510.13.4e-11048.99Eukaryotic aspartyl protease family protein [more]
AT3G18490.15.4e-10847.87Eukaryotic aspartyl protease family protein [more]
AT1G01300.18.6e-10644.79Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.1e-9942.60Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 118..305
e-value: 7.2E-51
score: 174.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 312..482
e-value: 1.5E-45
score: 157.1
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 139..482
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 327..478
e-value: 2.1E-33
score: 115.4
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 143..305
e-value: 1.9E-49
score: 168.3
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 56..482
NoneNo IPR availablePANTHERPTHR13683:SF265PROTEIN ASPARTIC PROTEASE IN GUARD CELL 2coord: 56..482
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 143..478
score: 45.799305
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 142..482
e-value: 3.08305E-133
score: 385.086

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0007530.1PI0007530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity