PI0007420 (gene) Melon (PI 482460) v1

Overview
NamePI0007420
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionMechanosensitive ion channel protein
Locationchr07: 23533050 .. 23535906 (+)
RNA-Seq ExpressionPI0007420
SyntenyPI0007420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTGAACGGGAATAAACCTATTCGAAGAAGTTCGTCTCAGAAGGAGAGTGAAAATGGAGGTGAAGTTGTGGTTCAGCTTAGCGGCGTTGAAAATGAGTACTCTGTTCCGAAGCAAAACAGAGTCGATTCACCGACCAAAGAGCCGACCGGGTCGAGCGTTGGCTATGGTGATGCTTCACCACTCGCTCCTACTGCAAATAAGCCACCGAAAATCCCAATTTCAAATGGAACCCTCACGCCAAGAAGGTCTCTGAGAAGATCAACTCTCTCCAAACCCAAATCCAGATTTGGGGAACAGCCAGGTTATATCGATTCAGACATGTTTGAGGAGGAGAATCATGTGTCGTTGAGAGAACAAATCGGAACTTCATCCAGAAGCTCAAATCTCAACACGCCGAAAGCCCACTGAGGGAGAAGATGATGGGAATGTGTGGGTTGAATAAAAAGCACAAGAAAGTGAAGGTGAAGACAGTGTTTAAGTGGATTGGAGTTTTTTGCATAATTAGTTGCTTGGTGGCTAGCTTGACTGTCAACCCTTTGAAGAATCGGTTCCTTTGGGGTTTGAAGGTTTGGAAATGGTGTTTACTTGCTACTGTGATTTTGTGTGGATTGATATTTACTCGTTGGGTTATGAATGTGGTTGTCTTTTTGATAGAGAGGAACTTTTTGCTTAAGAAAAAAGTACTTTATTTTGTTCATGGTTGAAGAAGAGTGTCCAAGTTACCCTTTGGTTGTCATTGGTTCTTGCTACATGGGGGTCCTTGTTCGATCGAAGAAACCATATGATTTCAAGTTCGAGGATCACTGCTAAGATTTTGGATGCTGTTACATGGACTTTGGCAAGCCTTCTTATAGGGGCATTCTTGTGGTTGATTAAAACATTGTTGCTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATGTCTTTTCCTTCATCATGTTCTACAAACCCTCTTGAGGCCTCTTCCAGTGGAGGATGAAAGTACTGCCAAGTTCAGGTGTTGTCGATTTTGTTTTGAGAGTAAAAAATCAGACCGTAAAAGGTGATTGACATGGAAAGATTCACCAGCTGGAGCGAGAGAAGGTTTCAGCTTGGACAATGAAGGTATTGGTTGATGCAGTTACTAGTTCAGAGATGTCGATCTCACAAATTCTTGATGATGAAAGCTACAGAGATGTCGCCGATGATGAGATCACAAACGAGATGAAAGTTGCCAAAGAAGCTGCTAAAGAGATCTTCAAAAATGTTGCTCTTCCTGGAAACAAGTGAGAAACTTTCCTTTTTGCTTGTGATCTTTTGTTCAAACTCATGTTAATATTTTACCATTTCCCTTTTGTTAGGTTCATAGAGGAAAGGGATCTTCAGGTATTCATGATTGCAGAAGAAGTTAAACTTGTGTGGCCACACTTTGAGGTAGATAAGACAAGAAGATTGACATGAAAGCTTTAACAAATTGGGTGGTGAGACCTTGTATCTGTTATTTATCTTTATTCATATTGAGTCATCTAGCCACTTGAACCTATTAAAGTGTCACTCGCTTAGGCTCCACTTACTGAATTTGAATAGAAACGAATCAAAGGAGAGTTTCCATTAAAATTAAGAGTCGTTTTGGATTTTGCTGTAGGATACACAAGTTCAAAAGCATTCTTATATTAGACAACAATTAGAATTATTTCTCGTTTGCTCATCTCCTAATAAGCAAACAAAGTCTTGGAATACAATAAATACATAACATAGACTTCTTATGGTCAAATGATTCAGAATGCTTAATTGGTTTGGCCAAACATTTAGTTCCTCTTACTTCCTTGTCTTCAATAATTTTCTAAAACATGTTTGAAGAACCAAAGCATGTTGATAATGTTCTATAGCACGTAGTAACTTCTTTCGTGCAGATGAAGGTTTATCAAGGGAGGAAAACTCTAGCACATGCCTTGAAAGACACTAAAACAGCAGTGAAGCAATTGAACAATTTAATAACAGCGCTTATTATAATAGTAACCGCTGTTATTTGGCTTCTGTTGATGGAAATTGCTACACCAAAGTACTTGTCTTCCTTCTAACTCAGCTTGCAGTGGCAGCTTTCATGTTTGGAAACACTTGCAAGACTGCATTTGAAGCTCTAATCTTTGTGTTTGTGATGCATCCATTTGATGTCGGGGACCGATGTGTTGTCGATGGCGTCCAGGTAAGCTTCTTAACTTACAGTCATCAAAGTCTCCTGTTTCTTTCCACCTTTAACTTGTGGCAATCGGCAGCTGTTGGTTGAAGAAATGAATATCTTGACAACAGTCTTCTTGAAACTCAACAATGAGAAGGTTTATTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAACTACTACAGAAGTCCAGACATGGGTGACACCATTCAATTCTCAATCGGTTTCGGAACACCATTGGAGAAGATTGGGGCCATGAAAGAACAAATAAAGAGGTGAGTGGAAGTGATACAAATAATGAAGATTTTGAATGCAGCTTTACAATATTTTGATCGTTCCAAAACAAAGAAAAATCATTGTTTCCAAATATATGATTTGATCCTTATCAATGGCTGCAGGTATTTGGAGGAGAATCCACAACACTGGTATCCAAATCATAGTGTGGTGGTGCAGGAGATCGAAAACGTGAATAAGATAAAGATCGCTCTTTATACGAACCACACCATGAATTTTCAAGACTGGGCTGAGAAGAACCGACGAAGAACCGAGCTTGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACAATCTTCTGCCTCAAACAGTTCATCTCTTCCCAGTTGAGGGGCACTGA

mRNA sequence

ATGGATGTGAACGGGAATAAACCTATTCGAAGAAGTTCGTCTCAGAAGGAGAGTGAAAATGGAGGTGAAGTTGTGGTTCAGCTTAGCGGCGTTGAAAATGAGTACTCTGTTCCGAAGCAAAACAGAGTCGATTCACCGACCAAAGAGCCGACCGGGTCGAGCGTTGGCTATGGTGATGCTTCACCACTCGCTCCTACTGCAAATAAGCCACCGAAAATCCCAATTTCAAATGGAACCCTCACGCCAAGAAGGTCTCTGAGAAGATCAACTCTCTCCAAACCCAAATCCAGATTTGGGGAACAGCCAGGTTATATCGATTCAGACATGTTTGAGGAGGAGAATCATAAGCTCAAATCTCAACACGCCGAAAGCCCACTGAGGGAGAAGATGATGGGAATGTGTGGGTTGAATAAAAAGCACAAGAAAGTGAAGGTGAAGACAGTGTTTAAGTGGATTGGAGTTTTTTGCATAATTAGTTGCTTGGTGGCTAGCTTGACTGTCAACCCTTTGAAGAATCGGTTCCTTTGGGGTTTGAAGGTTTGGAAATGGTGTTTACTTGCTACTGTGATTTTGTGTGGATTGATATTTACTCGTTGGGTTATGAATGTGGTTGTCTTTTTGATAGAGAGGAACTTTTTGCTTAAGAAAAAAAGTGTCCAAGTTACCCTTTGGTTGTCATTGGTTCTTGCTACATGGGGGTCCTTGTTCGATCGAAGAAACCATATGATTTCAAGTTCGAGGATCACTGCTAAGATTTTGGATGCTGTTACATGGACTTTGGCAAGCCTTCTTATAGGGGCATTCTTGTGGTTGATTAAAACATTGTTGCTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATGTCTTTTCCTTCATCATGTTCTACAAACCCTCTTGAGGCCTCTTCCAGTGGAGGATGAAAGTACTGCCAAGTTCAGGTGTTGTCGATTTTGTTTTGAGAGTAAAAAATCAGACCAGAAGGTTTCAGCTTGGACAATGAAGGTATTGGTTGATGCAGTTACTAGTTCAGAGATGTCGATCTCACAAATTCTTGATGATGAAAGCTACAGAGATGTCGCCGATGATGAGATCACAAACGAGATGAAAGTTGCCAAAGAAGCTGCTAAAGAGATCTTCAAAAATGTTGCTCTTCCTGGAAACAAGTTCATAGAGGAAAGGGATCTTCAGGTATTCATGATTGCAGAAGAAGTTAAACTTGTGTGGCCACACTTTGAGATGAAGGTTTATCAAGGGAGGAAAACTCTAGCACATGCCTTGAAAGACACTAAAACAGCAGTGAAGCAATTGAACAATTTAATAACAGCGCTTATTATAATAGTAACCGCTCTTGCAGTGGCAGCTTTCATGTTTGGAAACACTTGCAAGACTGCATTTGAAGCTCTAATCTTTGTGTTTGTGATGCATCCATTTGATGTCGGGGACCGATGTGTTGTCGATGGCGTCCAGCTGTTGGTTGAAGAAATGAATATCTTGACAACAGTCTTCTTGAAACTCAACAATGAGAAGGTTTATTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAACTACTACAGAAGTCCAGACATGGGTGACACCATTCAATTCTCAATCGGTTTCGGAACACCATTGGAGAAGATTGGGGCCATGAAAGAACAAATAAAGAGGTATTTGGAGGAGAATCCACAACACTGGTATCCAAATCATAGTGTGGTGGTGCAGGAGATCGAAAACGTGAATAAGATAAAGATCGCTCTTTATACGAACCACACCATGAATTTTCAAGACTGGGCTGAGAAGAACCGACGAAGAACCGAGCTTGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACAATCTTCTGCCTCAAACAGTTCATCTCTTCCCAGTTGAGGGGCACTGA

Coding sequence (CDS)

ATGGATGTGAACGGGAATAAACCTATTCGAAGAAGTTCGTCTCAGAAGGAGAGTGAAAATGGAGGTGAAGTTGTGGTTCAGCTTAGCGGCGTTGAAAATGAGTACTCTGTTCCGAAGCAAAACAGAGTCGATTCACCGACCAAAGAGCCGACCGGGTCGAGCGTTGGCTATGGTGATGCTTCACCACTCGCTCCTACTGCAAATAAGCCACCGAAAATCCCAATTTCAAATGGAACCCTCACGCCAAGAAGGTCTCTGAGAAGATCAACTCTCTCCAAACCCAAATCCAGATTTGGGGAACAGCCAGGTTATATCGATTCAGACATGTTTGAGGAGGAGAATCATAAGCTCAAATCTCAACACGCCGAAAGCCCACTGAGGGAGAAGATGATGGGAATGTGTGGGTTGAATAAAAAGCACAAGAAAGTGAAGGTGAAGACAGTGTTTAAGTGGATTGGAGTTTTTTGCATAATTAGTTGCTTGGTGGCTAGCTTGACTGTCAACCCTTTGAAGAATCGGTTCCTTTGGGGTTTGAAGGTTTGGAAATGGTGTTTACTTGCTACTGTGATTTTGTGTGGATTGATATTTACTCGTTGGGTTATGAATGTGGTTGTCTTTTTGATAGAGAGGAACTTTTTGCTTAAGAAAAAAAGTGTCCAAGTTACCCTTTGGTTGTCATTGGTTCTTGCTACATGGGGGTCCTTGTTCGATCGAAGAAACCATATGATTTCAAGTTCGAGGATCACTGCTAAGATTTTGGATGCTGTTACATGGACTTTGGCAAGCCTTCTTATAGGGGCATTCTTGTGGTTGATTAAAACATTGTTGCTGAAAATACTGGCCTCCAAGTTCCATATGAACCGATTTTTCGACAGAATTCAGGAATGTCTTTTCCTTCATCATGTTCTACAAACCCTCTTGAGGCCTCTTCCAGTGGAGGATGAAAGTACTGCCAAGTTCAGGTGTTGTCGATTTTGTTTTGAGAGTAAAAAATCAGACCAGAAGGTTTCAGCTTGGACAATGAAGGTATTGGTTGATGCAGTTACTAGTTCAGAGATGTCGATCTCACAAATTCTTGATGATGAAAGCTACAGAGATGTCGCCGATGATGAGATCACAAACGAGATGAAAGTTGCCAAAGAAGCTGCTAAAGAGATCTTCAAAAATGTTGCTCTTCCTGGAAACAAGTTCATAGAGGAAAGGGATCTTCAGGTATTCATGATTGCAGAAGAAGTTAAACTTGTGTGGCCACACTTTGAGATGAAGGTTTATCAAGGGAGGAAAACTCTAGCACATGCCTTGAAAGACACTAAAACAGCAGTGAAGCAATTGAACAATTTAATAACAGCGCTTATTATAATAGTAACCGCTCTTGCAGTGGCAGCTTTCATGTTTGGAAACACTTGCAAGACTGCATTTGAAGCTCTAATCTTTGTGTTTGTGATGCATCCATTTGATGTCGGGGACCGATGTGTTGTCGATGGCGTCCAGCTGTTGGTTGAAGAAATGAATATCTTGACAACAGTCTTCTTGAAACTCAACAATGAGAAGGTTTATTATCCCAACTCAGTTTTGGCAACAAAGCCCATCACTAACTACTACAGAAGTCCAGACATGGGTGACACCATTCAATTCTCAATCGGTTTCGGAACACCATTGGAGAAGATTGGGGCCATGAAAGAACAAATAAAGAGGTATTTGGAGGAGAATCCACAACACTGGTATCCAAATCATAGTGTGGTGGTGCAGGAGATCGAAAACGTGAATAAGATAAAGATCGCTCTTTATACGAACCACACCATGAATTTTCAAGACTGGGCTGAGAAGAACCGACGAAGAACCGAGCTTGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACAATCTTCTGCCTCAAACAGTTCATCTCTTCCCAGTTGAGGGGCACTGA

Protein sequence

MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDASPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHKLKSQHAESPLREKMMGMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFRCCRFCFESKKSDQKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFEMKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTALAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSVVVQEIENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEELKINYNLLPQTVHLFPVEGH
Homology
BLAST of PI0007420 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.9e-145
Identity = 330/735 (44.90%), Postives = 440/735 (59.86%), Query Frame = 0

Query: 13  SSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKE---PTGSSVGYGDASPLAPTANK 72
           + QK S  GG     +  V  E +  +   + SP  E   P   S    + S L  + NK
Sbjct: 2   AEQKSSNGGGGGGDVVINVPVEEASRRSKEMASPESEKGVPFSKSPS-PEISKLVGSPNK 61

Query: 73  PPKIPISNGT-LTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEE--------------- 132
           PP+ P  N   LT R+S  RS  SKPKSRF +    +D+ + EEE               
Sbjct: 62  PPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQLGAGFSFSRAS 121

Query: 133 --NHKLKSQHAESPLR-----------EKMMGMCGLNKK-HKKVKVKTVFKWIGVFCIIS 192
             N   +S  + +P+            E++     LN++   K+    + +      I+S
Sbjct: 122 PNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAFFVVILS 181

Query: 193 CLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK---- 252
            LVASLT+N LK+   WGL+VWKWC+L  VI  G++ T W M ++VFLIE NFLL+    
Sbjct: 182 ALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVL 241

Query: 253 ------KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFL 312
                 KKSVQV +WL L+L  W  LF   NH +  S    K+L  +T TL S+L GAF 
Sbjct: 242 YFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISILTGAFF 301

Query: 313 WLIKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFR-----CCR 372
           WL+KTLLLKILA+ F++N FFDRIQ+ +F  +VLQT L  LP+ +E+    R        
Sbjct: 302 WLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQT-LSGLPLMEEAERVGREPSTGHLS 361

Query: 373 FCFESKKSD-----------------QKVSAWTMKVLVDAVTSSEMS-ISQILDDESY-- 432
           F    KK                   +KVSAWTM+VL++AV +S +S IS  LD+ +Y  
Sbjct: 362 FATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGE 421

Query: 433 -RDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE--- 492
            ++ AD EIT+EM+ A  AA  +F+NVA P   +IEE DL  FMI EEV LV+P F+   
Sbjct: 422 GKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAA 481

Query: 493 --------------MKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTA--------- 552
                         +KVY  R+ LAH+L DTKTAVKQLN L+TA++++VT          
Sbjct: 482 ETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEV 541

Query: 553 ------------LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 612
                       L   AF+ G+TCK  FE+++FVFVMHP+DVGDRCVVDGV +LVEEMN+
Sbjct: 542 ATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNL 601

Query: 613 LTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKR 641
           LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+T++FSI F TP+ KI  +KE+I  
Sbjct: 602 LTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAE 661

BLAST of PI0007420 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 1.3e-133
Identity = 314/744 (42.20%), Postives = 423/744 (56.85%), Query Frame = 0

Query: 14  SQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDASPLAP-------- 73
           +++   NG EVV+ +S  E+    P+ +   +P   P  S  G   + P+ P        
Sbjct: 2   AERRVSNGEEVVINVSDKEDSKD-PRASPSFNPLASP-DSDAGIEKSKPVPPISIPTPEI 61

Query: 74  -----TANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGY-IDSDMFEEENHKLKS 133
                + +KPPKIP   G L  R+SL RS  SKPKSRFGEQ  +  DS   E     L+ 
Sbjct: 62  YKFSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLRE 121

Query: 134 QHAESPLREKMMGMCGLNKK------------------------HKKVKVKTV------- 193
           Q                N K                        +KKVK+  V       
Sbjct: 122 QFGAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMKP 181

Query: 194 --FKWIGVF-CIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVV 253
             F  + VF  I+  L+ SLT++ +    +WGL+ WKWC+L  V L G++ T W M+ VV
Sbjct: 182 LAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVV 241

Query: 254 FLIERNFLLK----------KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDA 313
           F+IE+N+LL+          KK+VQV +W SLVL  W  LFD     +  +R T + LD 
Sbjct: 242 FIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGD---VKRTRKTKRFLDF 301

Query: 314 VTWTLASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVED- 373
           +TWT+ SLL+G+ L+L+KT  LK+LASKF++  FF+RIQE +F  +VLQTL  P  +E+ 
Sbjct: 302 ITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEA 361

Query: 374 ------ESTAKFRCCRF---CFESKK----------SDQKVSAWTMKVLVDAV-TSSEMS 433
                  ST      R      + KK            +KVSAWTM+VL++AV TS   +
Sbjct: 362 ENVGRVPSTGHLSFTRTKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSGIST 421

Query: 434 ISQILDD-ESYRDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVK 493
           IS  LD+  + ++  D EITNEM+ A  AA ++F NVA P + +IEE DL  FMI EEV 
Sbjct: 422 ISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKEEVD 481

Query: 494 LVWPHFE----------------MKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVT- 553
           LV P  E                + VY  RKT+ H+L DTKTAVKQL+ LIT ++ ++T 
Sbjct: 482 LVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTVITF 541

Query: 554 -----ALAVA---------------AFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGV 613
                 L +A               AFM G+TCK  FE+ +FVFVMHP+DVGDRCVVDGV
Sbjct: 542 IVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVVDGV 601

Query: 614 QLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKI 641
            LLVEE+++LTTVFLK++NEKV+YPNSVL +KPI+N+YRSPDMGD + F I F TP EKI
Sbjct: 602 MLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPAEKI 661

BLAST of PI0007420 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.3e-97
Identity = 254/731 (34.75%), Postives = 372/731 (50.89%), Query Frame = 0

Query: 10  RRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDASPLAPTANK 69
           RR S    + N    VV+ SG     + P Q    +  K  T S +    + P  PT   
Sbjct: 136 RRDSRSNTNNNDDGEVVKCSG----NNAPIQRSSSTLLKMRTRSRL----SDP--PTPQL 195

Query: 70  PPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHKLKSQHAESPLREK 129
           PP+         P+    +S        FG+ P    +   EEE+    ++      R+ 
Sbjct: 196 PPQTADMKSGRIPKSGQMKSGF------FGKSP---KTQGEEEEDDPFAAEDLPEEYRK- 255

Query: 130 MMGMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATV 189
                       K+ +  V +W+ +  II+  V +L +  L+ + LW L++WKW  +  V
Sbjct: 256 -----------DKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLV 315

Query: 190 ILCGLIFTRWVMNVVVFLIERNFLLKK----------KSVQVTLWLSLVLATWGSLFDRR 249
           ++CG + + W++ +VVF IERNFLL+K          K+VQ  LWL LVL  W  LFD +
Sbjct: 316 LICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEK 375

Query: 250 NHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQECLFL 309
                +     K L  VT     LL+G  LWL+KTLL+K+LAS FHM+ +FDRIQE LF 
Sbjct: 376 ----VAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFT 435

Query: 310 HHVLQTLLRPLPVEDESTAKFRCCRFCFESKK---------------------------- 369
            +V++TL  P  +E +   +    R   E KK                            
Sbjct: 436 QYVIETLSGPPLIEIQKNEEEE-ERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSH 495

Query: 370 -----------------------SDQKVSAWTMKVLVDAVTSSEM-SISQILDDESYRDV 429
                                  + + VSAW MK L++ + +  + ++ + L D S  D 
Sbjct: 496 VLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDD 555

Query: 430 ADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE------- 489
             ++I +E + AK AA++IF NVA PG+KFI   D+  F+  +E       FE       
Sbjct: 556 KGNQIRSEFE-AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNR 615

Query: 490 ----------MKVYQGRKTLAHALKDTKTAVKQLN---NLITALIIIV------------ 549
                     +  ++ R+ LA  L DTKTAV +L+   N++  +II+V            
Sbjct: 616 ISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTK 675

Query: 550 ------TALAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTV 609
                 + + V AF+FGN CK  FE++I++FV+HPFDVGDRC +DGVQ++VEEMNILTTV
Sbjct: 676 FLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTV 735

Query: 610 FLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEE 641
           FL+ +N+KV YPNS+L TK I NYYRSPDMGD I+FSI   TP EKI  +K++I  Y+E 
Sbjct: 736 FLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEG 795

BLAST of PI0007420 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 6.8e-95
Identity = 221/631 (35.02%), Postives = 333/631 (52.77%), Query Frame = 0

Query: 120 QHAESPLREKMMGMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTVNPLKNRFLWGLK 179
           +  E P  E+ +   GL K  +K+ V  + +WI +  II+ L+ SL +  L+ + LW L 
Sbjct: 235 EEEEDPFSEEDLPE-GLRK--EKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLA 294

Query: 180 VWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKK----------KSVQVTLWLSLVL 239
           +WKW ++  V++CG + + W++ + V+ +E NFL +K          K VQ  LWL LVL
Sbjct: 295 LWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVL 354

Query: 240 ATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLLKILASKFHMNRF 299
             W  LFD++        + + +L  VT  L  LL+   +WLIKTLL+K+LAS FHM+ +
Sbjct: 355 IAWHFLFDKK----VEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTY 414

Query: 300 FDRIQECLFLHHVLQTLLRP----LPVEDESTAK-------------------------- 359
           FDRIQE LF  +V++TL  P    + +E+E  A                           
Sbjct: 415 FDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQV 474

Query: 360 ------------------------------FRCCRFCFESKKSDQKVSAWTMKVLVDAVT 419
                                             R     + + + VSAW MK L++ + 
Sbjct: 475 TVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIK 534

Query: 420 SSEMSI--SQILDDESYRDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFM 479
              +S    QI D  +  D    +I +E + AK AA++IF+NVA PG+++I   D   F+
Sbjct: 535 KGTLSTLDEQIQDTTTQEDDKATQIRSEFE-AKLAARKIFQNVAEPGSRYIYMEDFMRFL 594

Query: 480 IAEEVKLVWPHFE-----------------MKVYQGRKTLAHALKDTKTAVKQLNNLITA 539
             +E +     FE                 +  ++ R+ LA  L DTKTAV +L+ ++  
Sbjct: 595 SEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDV 654

Query: 540 LIIIV---------------------TALAVAAFMFGNTCKTAFEALIFVFVMHPFDVGD 599
           L+ IV                     + L +  F+FGN+CKT FEA+IFVFVMHPFDVGD
Sbjct: 655 LVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGD 714

Query: 600 RCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGF 641
           RC +DGVQ++VEEMNILTTVFL+ +N+K+ YPNS+L TKPI NYYRSPDM D I+F +  
Sbjct: 715 RCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHI 774

BLAST of PI0007420 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.2e-94
Identity = 251/736 (34.10%), Postives = 373/736 (50.68%), Query Frame = 0

Query: 6   NKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDASPLAP 65
           N+     SS +E  N  E  V   G  ++  +  +N+  S  ++P          +P  P
Sbjct: 159 NRTSLGGSSDEEGRNRDEAEVLKCG--SKKPMLSRNKTKSRLQDP---------PTPTHP 218

Query: 66  TANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQP--GYIDSDMFEEENHKLKSQHAE 125
             +K           T  +S RRS + K     G+ P  G    + FEEE    +    +
Sbjct: 219 AIDK-----------TEMKSGRRSGIFK-SGFLGKSPKAGTPGRNGFEEEEE--EDPFLD 278

Query: 126 SPLREKMMGMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKW 185
             L E+         K  K+      +WI +  I++ LV SLT++ L+ +  W L +WKW
Sbjct: 279 EDLPEEF--------KRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKW 338

Query: 186 CLLATVILCGLIFTRWVMNVVVFLIERNFLLKK----------KSVQVTLWLSLVLATWG 245
            +   V++CG + + W++ ++VFL+E+NF  +K          KSVQ  LWL LVL  W 
Sbjct: 339 EVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWH 398

Query: 246 SLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLLKILASKFHMNRFFDRI 305
            LFD++  +   +R TA  L  VT  L  LL+   +WL+KT+L+K+LAS FHM+ +FDRI
Sbjct: 399 FLFDKK--VERETRSTA--LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRI 458

Query: 306 QECLFLHHVLQTLLRP-----------------------------LPVEDESTAKF---- 365
           QE LF  +V++TL  P                             LP   ++T K     
Sbjct: 459 QESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKV 518

Query: 366 -----------------RCCRFCFESKKSDQKVSAWTMKVLVDAVTSSEMS-ISQILDDE 425
                               R     + + + VSAW MK L++ +    +S + Q + D 
Sbjct: 519 GKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDT 578

Query: 426 SYRDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE-- 485
           +  D     I +E + AK AA++IF NV  PG+++I   D   F+  EE +     FE  
Sbjct: 579 TQEDEDATHIRSEYE-AKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGA 638

Query: 486 ---------------MKVYQGRKTLAHALKDTKTAVKQLNNLITA--------------- 545
                          +K ++ R+ LA  L DTKTAV +L+ +I                 
Sbjct: 639 SESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILG 698

Query: 546 ------LIIIVTALAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMN 605
                 L+++ + L + AF+FGN+CKT FEA+IF+FVMHPFDVGDRC +DGVQL+VEEMN
Sbjct: 699 IATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMN 758

Query: 606 ILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIK 641
           ILTTVFL+ +N+K+ YPNSVL TKPI NYYRSPDMGD ++F +   TP EKI A+K++I 
Sbjct: 759 ILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRIL 818

BLAST of PI0007420 vs. ExPASy TrEMBL
Match: A0A1S4DZ48 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=3 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 603/721 (83.63%), Postives = 619/721 (85.85%), Query Frame = 0

Query: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDA 60
           MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDS TKEPTGSSVGYG+A
Sbjct: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGYGNA 60

Query: 61  SPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH-KLKS 120
           S LAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH  L+ 
Sbjct: 61  SQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVSLRE 120

Query: 121 QHAESPLREKMMGM------------CGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTV 180
           Q   +  R   +               GLN+KHKK KVKTVFKWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDGRNMGLNEKHKKAKVKTVFKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK----------KK 240
            PLKNRFLWGLKVWKWCLLATVI CGLIFTRW+MNVVVFLIERNFLLK          KK
Sbjct: 181 KPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFRCCRFCFESKKSD---- 360
           KILASKFHMNRFFDRIQE LFLHHVLQTLLRP PVEDESTAKFRCCRFCFESK+SD    
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDESTAKFRCCRFCFESKESDRKKV 360

Query: 361 -----------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNEMKVAKEA 420
                      +KVSAWTMKVLVDAVTSSEMSISQILDDESYRDVAD +ITNEM VAKEA
Sbjct: 361 IDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITNEMNVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE----------------MKVYQG 480
           AKEIFKNVALPGNKFIEERDL  FMI EEV LVWPHFE                +KVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------LAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALIIIVTA                     LAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSVVVQEI 647
           ATKPITNYYRSPDMGDT++FSIGF TP+E+IGAMKEQIKRYLEENPQHWYPNH+VVV+EI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

BLAST of PI0007420 vs. ExPASy TrEMBL
Match: A0A5D3D991 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001820 PE=3 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 603/721 (83.63%), Postives = 619/721 (85.85%), Query Frame = 0

Query: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDA 60
           MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDS TKEPTGSSVGYG+A
Sbjct: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGYGNA 60

Query: 61  SPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH-KLKS 120
           S LAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH  L+ 
Sbjct: 61  SQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVSLRE 120

Query: 121 QHAESPLREKMMGM------------CGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTV 180
           Q   +  R   +               GLN+KHKK KVKTVFKWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDGRNMGLNEKHKKAKVKTVFKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK----------KK 240
            PLKNRFLWGLKVWKWCLLATVI CGLIFTRW+MNVVVFLIERNFLLK          KK
Sbjct: 181 KPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFRCCRFCFESKKSD---- 360
           KILASKFHMNRFFDRIQE LFLHHVLQTLLRP PVEDESTAKFRCCRFCFESK+SD    
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDESTAKFRCCRFCFESKESDRKKV 360

Query: 361 -----------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNEMKVAKEA 420
                      +KVSAWTMKVLVDAVTSSEMSISQILDDESYRDVAD +ITNEM VAKEA
Sbjct: 361 IDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITNEMNVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE----------------MKVYQG 480
           AKEIFKNVALPGNKFIEERDL  FMI EEV LVWPHFE                +KVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------LAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALIIIVTA                     LAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSVVVQEI 647
           ATKPITNYYRSPDMGDT++FSIGF TP+E+IGAMKEQIKRYLEENPQHWYPNH+VVV+EI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

BLAST of PI0007420 vs. ExPASy TrEMBL
Match: A0A0A0L5E6 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_4G647440 PE=3 SV=1)

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 591/721 (81.97%), Postives = 609/721 (84.47%), Query Frame = 0

Query: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDA 60
           MDV+GNK IRR SSQKES NGGEVVVQLSGVENE SVPKQNR DS TKEPTGSSVGY +A
Sbjct: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60

Query: 61  SPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH-KLKS 120
           SPLAPT NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQ  +IDSDM EEENH  L+ 
Sbjct: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120

Query: 121 QHAESPLREKMM------------GMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTV 180
           Q   +  R   +            G  G N KHKKVKVKTV KWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK----------KK 240
           NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK          KK
Sbjct: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLAS LIGAFLWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFRCCRFCFESKKSD---- 360
           KILASKFHMNRFFDRIQE LFLHHVLQTLLRP  VEDESTAKFRCCRFCFESKK D    
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360

Query: 361 -----------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNEMKVAKEA 420
                      +KVSAWTMKVLVDAVTSSEMS+SQILDDESYRDVAD +ITNEMKVAKEA
Sbjct: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE----------------MKVYQG 480
           AKEIFKNVALPGNKFIEERDL  FMI EEV LVWPHFE                +KVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------LAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALII+VTA                     LAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSVVVQEI 647
           ATKPITNYYRSPDMGDT++FSIGF TP+E+IGAMKEQIKRYLEENPQHWYPNH+VVV+EI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

BLAST of PI0007420 vs. ExPASy TrEMBL
Match: A0A5D3D919 (Mechanosensitive ion channel protein 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001770 PE=4 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.8e-255
Identity = 504/714 (70.59%), Postives = 552/714 (77.31%), Query Frame = 0

Query: 1   MDVNGNKP---IRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGY 60
           MDVN + P   + RSS  KESE+GG+ VV+LS +EN  SVP+QN + S T E   SS+ Y
Sbjct: 2   MDVNISNPSKVVPRSSFPKESEDGGQFVVELSSIENGCSVPEQN-LGSQTNEFIDSSISY 61

Query: 61  GDASPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH-K 120
            + S L   ANKP  IP SNG LT    LRR+ LSK KSRFG QP Y DS+M EEEN+  
Sbjct: 62  DNDSQL---ANKPQNIPSSNGNLT----LRRAILSKSKSRFGVQPVYTDSNMCEEENYPS 121

Query: 121 LKSQHAESPLRE-----KMMGMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTVNPLK 180
            + Q  E+  R      +       ++KHKKVKVKTV KWIGVFCIISCLVASLTV+PLK
Sbjct: 122 SREQIGETSSRSFTHNTQKATPERKDEKHKKVKVKTVIKWIGVFCIISCLVASLTVDPLK 181

Query: 181 NRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK----------KKSVQV 240
           NRFLWGLKVWKWCLLATVILCGL+FTRWVMNVVVFLIE+NFLLK          KKSVQV
Sbjct: 182 NRFLWGLKVWKWCLLATVILCGLLFTRWVMNVVVFLIEKNFLLKKKVLYFVHGLKKSVQV 241

Query: 241 TLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLLKILA 300
           TLWL+LV ATW SLFDRRNH +S+SRIT+K+LD VTWTL SLLIGAFLWLIKTLLLKILA
Sbjct: 242 TLWLTLVFATWESLFDRRNHTVSNSRITSKVLDFVTWTLVSLLIGAFLWLIKTLLLKILA 301

Query: 301 SKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFRCCRFCFESKKSD-------- 360
           SKFHMNRFFDRIQE LF HH+LQ LL     EDES A+FRCCRF  ESKKSD        
Sbjct: 302 SKFHMNRFFDRIQESLFHHHILQMLLMARTQEDESYAEFRCCRFPSESKKSDCQKVIDIE 361

Query: 361 -------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNEMKVAKEAAKEI 420
                  +KVSAW MK LVDAVTSSEMSIS+ L DESYR+ AD EIT+EMKVAK+AAK+I
Sbjct: 362 KIHQLKREKVSAWKMKTLVDAVTSSEMSISKAL-DESYRNAADGEITDEMKVAKQAAKKI 421

Query: 421 FKNVALPGNKFIEERDLQVFMIAE-EVKLVWPHFE----------------MKVYQGRKT 480
           FKNVA PG KF+EE+DL  FMI E EV L+WPHFE                +KVYQGRKT
Sbjct: 422 FKNVA-PGKKFLEEKDLLKFMIDEAEVNLLWPHFEVDKTKKIDMKALTNWVVKVYQGRKT 481

Query: 481 LAHALKDTKTAVKQLNNLITALIIIVTAL---------------------AVAAFMFGNT 540
           LAHALKDTKTAVKQL+NL+ ALI+IVTA+                     AVAAFMFGNT
Sbjct: 482 LAHALKDTKTAVKQLDNLVAALIVIVTAVIWLLLMEIATTKVLVFLLTQFAVAAFMFGNT 541

Query: 541 CKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATK 600
           CK  FE LIFVFVMHPFDVGD CVVDG+QLLVEEMNILTTVFLKLNNEKVYYPNSVLATK
Sbjct: 542 CKNTFEGLIFVFVMHPFDVGDLCVVDGIQLLVEEMNILTTVFLKLNNEKVYYPNSVLATK 601

Query: 601 PITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSVVVQEIENV 643
           PITNYYRSPDMGDTI+FSI F TPLEKIG MKE+IKRYLE+NPQHWYPNHSVVV+EIENV
Sbjct: 602 PITNYYRSPDMGDTIEFSISFTTPLEKIGIMKEKIKRYLEDNPQHWYPNHSVVVKEIENV 661

BLAST of PI0007420 vs. ExPASy TrEMBL
Match: A0A6J1J491 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111481199 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 5.4e-252
Identity = 490/728 (67.31%), Postives = 549/728 (75.41%), Query Frame = 0

Query: 1   MDVNGN---KPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGY 60
           MD+NGN   KPIRRSSSQKESENG +VVV++S  EN +SVPKQNRVDS TKEP  SS+GY
Sbjct: 1   MDLNGNTPLKPIRRSSSQKESENGSQVVVEISRDENGHSVPKQNRVDSQTKEPMDSSIGY 60

Query: 61  GDASPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHKL 120
           G  S L PTANKPPKIP S GTLTPR+SL+RS LSKPKSRFGEQ   IDSD FEE     
Sbjct: 61  GYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSVIDSDTFEENRLSF 120

Query: 121 KSQHAESPLREKMMGM------------CGLNK----KHKKVKVKTVFKWIGVFCIISCL 180
           + Q + +  R   + M              + K    KHKKVK  T+ KW+G FCII CL
Sbjct: 121 RDQISANSSRRSTLNMPKEHHEEGDDEFFKIEKFKKVKHKKVKAITLIKWVGAFCIIGCL 180

Query: 181 VASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK------ 240
           VASLT+  LKN FLWG+++WKWCLLATVILCG+IFT  VMNV+VFLIERNFLL+      
Sbjct: 181 VASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLIERNFLLQKKVFYF 240

Query: 241 ----KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWL 300
               KKSVQVTLWL+LVL TW SLFDR +H +  S+I+ KILDA+TWTL +LLIGAFLWL
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITWTLITLLIGAFLWL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPV-EDESTAKFRCCRFCFESK 360
           IKTLLLKILASKFHMNRFFDRIQE +F HHVLQTLL P P+   EST +    R  F+ K
Sbjct: 301 IKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPESTVESNSGRLSFKGK 360

Query: 361 KSD---------------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNE 420
           KSD               +KVSAWTMKVLVDAV SSEMSISQ+L DESY+ VAD EI +E
Sbjct: 361 KSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLL-DESYQ-VADGEIADE 420

Query: 421 MKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE--------------- 480
           M+VA  AA +IF N+A+PGN FIEE DL+  MI EEV LV P FE               
Sbjct: 421 MEVAGYAAYKIFDNIAVPGNDFIEEEDLRRVMIKEEVDLVLPLFEVDETRRIDWKSLTNW 480

Query: 481 -MKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------L 540
            +KVY+ RKTLAHALKDTKTAVKQLNNL+TAL+IIVTA                     L
Sbjct: 481 VLKVYKERKTLAHALKDTKTAVKQLNNLVTALLIIVTAIIWLLLMEIATTKVLVFLLSQL 540

Query: 541 AVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKV 600
           AVA FMFGNTCKT FEAL+FVFVMHPFDVGDRC V+GV L+VEEMNILTTVFLKLNNEKV
Sbjct: 541 AVAVFMFGNTCKTIFEALVFVFVMHPFDVGDRCAVEGVPLMVEEMNILTTVFLKLNNEKV 600

Query: 601 YYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNH 647
           YYPNSVLATK ITNYYRSPDM DT++FSIGF TPLE+IG MK++IKRYLE+NPQHW+PNH
Sbjct: 601 YYPNSVLATKSITNYYRSPDMSDTVEFSIGFATPLERIGLMKDRIKRYLEKNPQHWHPNH 660

BLAST of PI0007420 vs. NCBI nr
Match: XP_008452779.1 (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_016901266.1 PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >KAA0064530.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa] >TYK20060.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 603/721 (83.63%), Postives = 619/721 (85.85%), Query Frame = 0

Query: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDA 60
           MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDS TKEPTGSSVGYG+A
Sbjct: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSQTKEPTGSSVGYGNA 60

Query: 61  SPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH-KLKS 120
           S LAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH  L+ 
Sbjct: 61  SQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHVSLRE 120

Query: 121 QHAESPLREKMMGM------------CGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTV 180
           Q   +  R   +               GLN+KHKK KVKTVFKWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDGRNMGLNEKHKKAKVKTVFKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK----------KK 240
            PLKNRFLWGLKVWKWCLLATVI CGLIFTRW+MNVVVFLIERNFLLK          KK
Sbjct: 181 KPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFRCCRFCFESKKSD---- 360
           KILASKFHMNRFFDRIQE LFLHHVLQTLLRP PVEDESTAKFRCCRFCFESK+SD    
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDESTAKFRCCRFCFESKESDRKKV 360

Query: 361 -----------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNEMKVAKEA 420
                      +KVSAWTMKVLVDAVTSSEMSISQILDDESYRDVAD +ITNEM VAKEA
Sbjct: 361 IDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADGDITNEMNVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE----------------MKVYQG 480
           AKEIFKNVALPGNKFIEERDL  FMI EEV LVWPHFE                +KVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------LAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALIIIVTA                     LAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSVVVQEI 647
           ATKPITNYYRSPDMGDT++FSIGF TP+E+IGAMKEQIKRYLEENPQHWYPNH+VVV+EI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

BLAST of PI0007420 vs. NCBI nr
Match: KAE8649918.1 (hypothetical protein Csa_012502 [Cucumis sativus])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 591/721 (81.97%), Postives = 609/721 (84.47%), Query Frame = 0

Query: 1    MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDA 60
            MDV+GNK IRR SSQKES NGGEVVVQLSGVENE SVPKQNR DS TKEPTGSSVGY +A
Sbjct: 738  MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 797

Query: 61   SPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH-KLKS 120
            SPLAPT NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQ  +IDSDM EEENH  L+ 
Sbjct: 798  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 857

Query: 121  QHAESPLREKMM------------GMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTV 180
            Q   +  R   +            G  G N KHKKVKVKTV KWIGVFCIISCLVASLTV
Sbjct: 858  QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 917

Query: 181  NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK----------KK 240
            NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK          KK
Sbjct: 918  NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 977

Query: 241  SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300
            SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLAS LIGAFLWLIKTLLL
Sbjct: 978  SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 1037

Query: 301  KILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFRCCRFCFESKKSD---- 360
            KILASKFHMNRFFDRIQE LFLHHVLQTLLRP  VEDESTAKFRCCRFCFESKK D    
Sbjct: 1038 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 1097

Query: 361  -----------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNEMKVAKEA 420
                       +KVSAWTMKVLVDAVTSSEMS+SQILDDESYRDVAD +ITNEMKVAKEA
Sbjct: 1098 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 1157

Query: 421  AKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE----------------MKVYQG 480
            AKEIFKNVALPGNKFIEERDL  FMI EEV LVWPHFE                +KVYQG
Sbjct: 1158 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 1217

Query: 481  RKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------LAVAAFMF 540
            RKTLAHALKDTKTAVKQLNNLITALII+VTA                     LAVAAFMF
Sbjct: 1218 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 1277

Query: 541  GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
            GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 1278 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 1337

Query: 601  ATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSVVVQEI 647
            ATKPITNYYRSPDMGDT++FSIGF TP+E+IGAMKEQIKRYLEENPQHWYPNH+VVV+EI
Sbjct: 1338 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 1397

BLAST of PI0007420 vs. NCBI nr
Match: XP_004141433.2 (mechanosensitive ion channel protein 10 [Cucumis sativus])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 591/721 (81.97%), Postives = 609/721 (84.47%), Query Frame = 0

Query: 1   MDVNGNKPIRRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDA 60
           MDV+GNK IRR SSQKES NGGEVVVQLSGVENE SVPKQNR DS TKEPTGSSVGY +A
Sbjct: 1   MDVSGNKGIRRGSSQKESGNGGEVVVQLSGVENECSVPKQNRADSQTKEPTGSSVGYDNA 60

Query: 61  SPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENH-KLKS 120
           SPLAPT NKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQ  +IDSDM EEENH  L+ 
Sbjct: 61  SPLAPTPNKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQSCFIDSDMLEEENHVSLRE 120

Query: 121 QHAESPLREKMM------------GMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTV 180
           Q   +  R   +            G  G N KHKKVKVKTV KWIGVFCIISCLVASLTV
Sbjct: 121 QIGATSSRSSNLNTPKAQPEGEDDGNMGSNAKHKKVKVKTVIKWIGVFCIISCLVASLTV 180

Query: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK----------KK 240
           NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK          KK
Sbjct: 181 NPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFVHGLKK 240

Query: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLL 300
           SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLAS LIGAFLWLIKTLLL
Sbjct: 241 SVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLIKTLLL 300

Query: 301 KILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFRCCRFCFESKKSD---- 360
           KILASKFHMNRFFDRIQE LFLHHVLQTLLRP  VEDESTAKFRCCRFCFESKK D    
Sbjct: 301 KILASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDESTAKFRCCRFCFESKKPDRKKV 360

Query: 361 -----------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNEMKVAKEA 420
                      +KVSAWTMKVLVDAVTSSEMS+SQILDDESYRDVAD +ITNEMKVAKEA
Sbjct: 361 IDMGKIHELKREKVSAWTMKVLVDAVTSSEMSVSQILDDESYRDVADGDITNEMKVAKEA 420

Query: 421 AKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE----------------MKVYQG 480
           AKEIFKNVALPGNKFIEERDL  FMI EEV LVWPHFE                +KVYQG
Sbjct: 421 AKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKIDMKALTNWVVKVYQG 480

Query: 481 RKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------LAVAAFMF 540
           RKTLAHALKDTKTAVKQLNNLITALII+VTA                     LAVAAFMF
Sbjct: 481 RKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKVLVFLLTQLAVAAFMF 540

Query: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600
           GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL
Sbjct: 541 GNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVL 600

Query: 601 ATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSVVVQEI 647
           ATKPITNYYRSPDMGDT++FSIGF TP+E+IGAMKEQIKRYLEENPQHWYPNH+VVV+EI
Sbjct: 601 ATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEENPQHWYPNHNVVVKEI 660

BLAST of PI0007420 vs. NCBI nr
Match: XP_038899727.1 (mechanosensitive ion channel protein 10-like isoform X1 [Benincasa hispida] >XP_038899728.1 mechanosensitive ion channel protein 10-like isoform X1 [Benincasa hispida])

HSP 1 Score: 942.2 bits (2434), Expect = 2.4e-270
Identity = 526/728 (72.25%), Postives = 568/728 (78.02%), Query Frame = 0

Query: 1   MDVNGNKPIR---RSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGY 60
           MDVN N+ ++    S SQ ESENGG+V V +S VEN YSVPKQNRVDS TKEP  SS+GY
Sbjct: 1   MDVNSNRALKVVGGSLSQMESENGGQVAVNISSVENGYSVPKQNRVDSQTKEPIDSSIGY 60

Query: 61  GDASPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHKL 120
              S LAPTANKPPKIP S GTLTPRRSLRR  LSK KSRFGEQPGYIDSD+FEE +  L
Sbjct: 61  SYDSQLAPTANKPPKIPSSKGTLTPRRSLRRPILSKSKSRFGEQPGYIDSDVFEENHVSL 120

Query: 121 KSQHAESPLREKMMG----------------MCGLNKKHKKVKVKTVFKWIGVFCIISCL 180
           + Q   +  R   +                 +  LN KHKKVKVKTV +WIGVFCII CL
Sbjct: 121 REQIGSTSSRSSTVNTPKAQPEEEDDAGIFEIEQLNNKHKKVKVKTVIEWIGVFCIIGCL 180

Query: 181 VASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK------ 240
           VASLTVNP KNRFLWGL+VWKW LLATVILCG+IFTRWVMNV+VFLIERNFLLK      
Sbjct: 181 VASLTVNPWKNRFLWGLRVWKWLLLATVILCGVIFTRWVMNVIVFLIERNFLLKKKVLYF 240

Query: 241 ----KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWL 300
               KKSVQVTLWL+LVL TWGSLFD+RNH I SSRITAKILD+VTWTL SLLIGA L L
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWGSLFDQRNHKI-SSRITAKILDSVTWTLVSLLIGAVLLL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPV-EDESTAKFRCCRFCFESK 360
           +KTLLLKILASKFHMNRFFDR+QE +FLH+VLQTL R + + E  +T+K    R   +SK
Sbjct: 301 VKTLLLKILASKFHMNRFFDRVQESIFLHYVLQTLSRGVFIGEAGTTSKSSSGRLSLKSK 360

Query: 361 KSD---------------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNE 420
           KSD               +KVSAWTMKVLVDAVTSSEMS+SQIL DESY+DVAD EI +E
Sbjct: 361 KSDRKEVIDLGKIHQLKREKVSAWTMKVLVDAVTSSEMSVSQIL-DESYQDVADGEIADE 420

Query: 421 MKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE--------------- 480
           M+VAK AA +IF NVALPGNKFIEE DL  FMI EEV LV PHFE               
Sbjct: 421 MEVAKAAAYKIFNNVALPGNKFIEEEDLLKFMIKEEVDLVLPHFEIDDTRRIDKKALTNW 480

Query: 481 -MKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------L 540
            +KVYQGRKTLAHALKDTKTAVKQLNNL+TALIIIVTA                     L
Sbjct: 481 VVKVYQGRKTLAHALKDTKTAVKQLNNLVTALIIIVTAVIWLLLMEIATTKVLVFLLTQL 540

Query: 541 AVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKV 600
           AVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKV
Sbjct: 541 AVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKV 600

Query: 601 YYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNH 647
           YYPNSVLATKPITNYYRSPDMG+TI+FSIGF TPLE+IG MK++IKRYLE+N QHWYPNH
Sbjct: 601 YYPNSVLATKPITNYYRSPDMGETIEFSIGFTTPLERIGVMKDKIKRYLEKNSQHWYPNH 660

BLAST of PI0007420 vs. NCBI nr
Match: XP_038899729.1 (mechanosensitive ion channel protein 10-like isoform X2 [Benincasa hispida])

HSP 1 Score: 940.6 bits (2430), Expect = 6.9e-270
Identity = 525/726 (72.31%), Postives = 567/726 (78.10%), Query Frame = 0

Query: 1   MDVNGNKPIR---RSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGY 60
           MDVN N+ ++    S SQ ESENGG+V V +S VEN YSVPKQNRVDS TKEP  SS+GY
Sbjct: 1   MDVNSNRALKVVGGSLSQMESENGGQVAVNISSVENGYSVPKQNRVDSQTKEPIDSSIGY 60

Query: 61  GDASPLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHKL 120
              S LAPTANKPPKIP S GTLTPRRSLRR  LSK KSRFGEQPGYIDSD+FEE +  L
Sbjct: 61  SYDSQLAPTANKPPKIPSSKGTLTPRRSLRRPILSKSKSRFGEQPGYIDSDVFEENHVSL 120

Query: 121 KSQHAESPLREKMMG----------------MCGLNKKHKKVKVKTVFKWIGVFCIISCL 180
           + Q   +  R   +                 +  LN KHKKVKVKTV +WIGVFCII CL
Sbjct: 121 REQIGSTSSRSSTVNTPKAQPEEEDDAGIFEIEQLNNKHKKVKVKTVIEWIGVFCIIGCL 180

Query: 181 VASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK------ 240
           VASLTVNP KNRFLWGL+VWKW LLATVILCG+IFTRWVMNV+VFLIERNFLLK      
Sbjct: 181 VASLTVNPWKNRFLWGLRVWKWLLLATVILCGVIFTRWVMNVIVFLIERNFLLKKKVLYF 240

Query: 241 ----KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFLWL 300
               KKSVQVTLWL+LVL TWGSLFD+RNH I SSRITAKILD+VTWTL SLLIGA L L
Sbjct: 241 VHGLKKSVQVTLWLTLVLVTWGSLFDQRNHKI-SSRITAKILDSVTWTLVSLLIGAVLLL 300

Query: 301 IKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPV-EDESTAKFRCCRFCFESK 360
           +KTLLLKILASKFHMNRFFDR+QE +FLH+VLQTL R + + E  +T+K    R   +SK
Sbjct: 301 VKTLLLKILASKFHMNRFFDRVQESIFLHYVLQTLSRGVFIGEAGTTSKSSSGRLSLKSK 360

Query: 361 KSD---------------QKVSAWTMKVLVDAVTSSEMSISQILDDESYRDVADDEITNE 420
           KSD               +KVSAWTMKVLVDAVTSSEMS+SQIL DESY+DVAD EI +E
Sbjct: 361 KSDRKEVIDLGKIHQLKREKVSAWTMKVLVDAVTSSEMSVSQIL-DESYQDVADGEIADE 420

Query: 421 MKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFEMK------------- 480
           M+VAK AA +IF NVALPGNKFIEE DL  FMI EEV LV PHFE+              
Sbjct: 421 MEVAKAAAYKIFNNVALPGNKFIEEEDLLKFMIKEEVDLVLPHFEIDDTRRIDKKALTNW 480

Query: 481 -VYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTA---------------------LAV 540
            VYQGRKTLAHALKDTKTAVKQLNNL+TALIIIVTA                     LAV
Sbjct: 481 VVYQGRKTLAHALKDTKTAVKQLNNLVTALIIIVTAVIWLLLMEIATTKVLVFLLTQLAV 540

Query: 541 AAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYY 600
           AAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYY
Sbjct: 541 AAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYY 600

Query: 601 PNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEENPQHWYPNHSV 647
           PNSVLATKPITNYYRSPDMG+TI+FSIGF TPLE+IG MK++IKRYLE+N QHWYPNHSV
Sbjct: 601 PNSVLATKPITNYYRSPDMGETIEFSIGFTTPLERIGVMKDKIKRYLEKNSQHWYPNHSV 660

BLAST of PI0007420 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 517.7 bits (1332), Expect = 1.3e-146
Identity = 330/735 (44.90%), Postives = 440/735 (59.86%), Query Frame = 0

Query: 13  SSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKE---PTGSSVGYGDASPLAPTANK 72
           + QK S  GG     +  V  E +  +   + SP  E   P   S    + S L  + NK
Sbjct: 2   AEQKSSNGGGGGGDVVINVPVEEASRRSKEMASPESEKGVPFSKSPS-PEISKLVGSPNK 61

Query: 73  PPKIPISNGT-LTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEE--------------- 132
           PP+ P  N   LT R+S  RS  SKPKSRF +    +D+ + EEE               
Sbjct: 62  PPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQLGAGFSFSRAS 121

Query: 133 --NHKLKSQHAESPLR-----------EKMMGMCGLNKK-HKKVKVKTVFKWIGVFCIIS 192
             N   +S  + +P+            E++     LN++   K+    + +      I+S
Sbjct: 122 PNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAFFVVILS 181

Query: 193 CLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK---- 252
            LVASLT+N LK+   WGL+VWKWC+L  VI  G++ T W M ++VFLIE NFLL+    
Sbjct: 182 ALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVL 241

Query: 253 ------KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFL 312
                 KKSVQV +WL L+L  W  LF   NH +  S    K+L  +T TL S+L GAF 
Sbjct: 242 YFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISILTGAFF 301

Query: 313 WLIKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFR-----CCR 372
           WL+KTLLLKILA+ F++N FFDRIQ+ +F  +VLQT L  LP+ +E+    R        
Sbjct: 302 WLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQT-LSGLPLMEEAERVGREPSTGHLS 361

Query: 373 FCFESKKSD-----------------QKVSAWTMKVLVDAVTSSEMS-ISQILDDESY-- 432
           F    KK                   +KVSAWTM+VL++AV +S +S IS  LD+ +Y  
Sbjct: 362 FATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGE 421

Query: 433 -RDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE--- 492
            ++ AD EIT+EM+ A  AA  +F+NVA P   +IEE DL  FMI EEV LV+P F+   
Sbjct: 422 GKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAA 481

Query: 493 --------------MKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTA--------- 552
                         +KVY  R+ LAH+L DTKTAVKQLN L+TA++++VT          
Sbjct: 482 ETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEV 541

Query: 553 ------------LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 612
                       L   AF+ G+TCK  FE+++FVFVMHP+DVGDRCVVDGV +LVEEMN+
Sbjct: 542 ATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNL 601

Query: 613 LTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKR 641
           LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+T++FSI F TP+ KI  +KE+I  
Sbjct: 602 LTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAE 661

BLAST of PI0007420 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 517.7 bits (1332), Expect = 1.3e-146
Identity = 330/735 (44.90%), Postives = 440/735 (59.86%), Query Frame = 0

Query: 13  SSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKE---PTGSSVGYGDASPLAPTANK 72
           + QK S  GG     +  V  E +  +   + SP  E   P   S    + S L  + NK
Sbjct: 2   AEQKSSNGGGGGGDVVINVPVEEASRRSKEMASPESEKGVPFSKSPS-PEISKLVGSPNK 61

Query: 73  PPKIPISNGT-LTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEE--------------- 132
           PP+ P  N   LT R+S  RS  SKPKSRF +    +D+ + EEE               
Sbjct: 62  PPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQLGAGFSFSRAS 121

Query: 133 --NHKLKSQHAESPLR-----------EKMMGMCGLNKK-HKKVKVKTVFKWIGVFCIIS 192
             N   +S  + +P+            E++     LN++   K+    + +      I+S
Sbjct: 122 PNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAFFVVILS 181

Query: 193 CLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK---- 252
            LVASLT+N LK+   WGL+VWKWC+L  VI  G++ T W M ++VFLIE NFLL+    
Sbjct: 182 ALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVL 241

Query: 253 ------KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFL 312
                 KKSVQV +WL L+L  W  LF   NH +  S    K+L  +T TL S+L GAF 
Sbjct: 242 YFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISILTGAFF 301

Query: 313 WLIKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFR-----CCR 372
           WL+KTLLLKILA+ F++N FFDRIQ+ +F  +VLQT L  LP+ +E+    R        
Sbjct: 302 WLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQT-LSGLPLMEEAERVGREPSTGHLS 361

Query: 373 FCFESKKSD-----------------QKVSAWTMKVLVDAVTSSEMS-ISQILDDESY-- 432
           F    KK                   +KVSAWTM+VL++AV +S +S IS  LD+ +Y  
Sbjct: 362 FATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGE 421

Query: 433 -RDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE--- 492
            ++ AD EIT+EM+ A  AA  +F+NVA P   +IEE DL  FMI EEV LV+P F+   
Sbjct: 422 GKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAA 481

Query: 493 --------------MKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTA--------- 552
                         +KVY  R+ LAH+L DTKTAVKQLN L+TA++++VT          
Sbjct: 482 ETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEV 541

Query: 553 ------------LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 612
                       L   AF+ G+TCK  FE+++FVFVMHP+DVGDRCVVDGV +LVEEMN+
Sbjct: 542 ATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNL 601

Query: 613 LTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKR 641
           LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+T++FSI F TP+ KI  +KE+I  
Sbjct: 602 LTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAE 661

BLAST of PI0007420 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 517.7 bits (1332), Expect = 1.3e-146
Identity = 330/735 (44.90%), Postives = 440/735 (59.86%), Query Frame = 0

Query: 13  SSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKE---PTGSSVGYGDASPLAPTANK 72
           + QK S  GG     +  V  E +  +   + SP  E   P   S    + S L  + NK
Sbjct: 2   AEQKSSNGGGGGGDVVINVPVEEASRRSKEMASPESEKGVPFSKSPS-PEISKLVGSPNK 61

Query: 73  PPKIPISNGT-LTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEE--------------- 132
           PP+ P  N   LT R+S  RS  SKPKSRF +    +D+ + EEE               
Sbjct: 62  PPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQLGAGFSFSRAS 121

Query: 133 --NHKLKSQHAESPLR-----------EKMMGMCGLNKK-HKKVKVKTVFKWIGVFCIIS 192
             N   +S  + +P+            E++     LN++   K+    + +      I+S
Sbjct: 122 PNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTLALIESAFFVVILS 181

Query: 193 CLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLK---- 252
            LVASLT+N LK+   WGL+VWKWC+L  VI  G++ T W M ++VFLIE NFLL+    
Sbjct: 182 ALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVL 241

Query: 253 ------KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASLLIGAFL 312
                 KKSVQV +WL L+L  W  LF   NH +  S    K+L  +T TL S+L GAF 
Sbjct: 242 YFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTLISILTGAFF 301

Query: 313 WLIKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVEDESTAKFR-----CCR 372
           WL+KTLLLKILA+ F++N FFDRIQ+ +F  +VLQT L  LP+ +E+    R        
Sbjct: 302 WLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQT-LSGLPLMEEAERVGREPSTGHLS 361

Query: 373 FCFESKKSD-----------------QKVSAWTMKVLVDAVTSSEMS-ISQILDDESY-- 432
           F    KK                   +KVSAWTM+VL++AV +S +S IS  LD+ +Y  
Sbjct: 362 FATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGE 421

Query: 433 -RDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE--- 492
            ++ AD EIT+EM+ A  AA  +F+NVA P   +IEE DL  FMI EEV LV+P F+   
Sbjct: 422 GKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAA 481

Query: 493 --------------MKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTA--------- 552
                         +KVY  R+ LAH+L DTKTAVKQLN L+TA++++VT          
Sbjct: 482 ETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEV 541

Query: 553 ------------LAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 612
                       L   AF+ G+TCK  FE+++FVFVMHP+DVGDRCVVDGV +LVEEMN+
Sbjct: 542 ATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNL 601

Query: 613 LTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKR 641
           LTTVFLKLNNEKVYYPN+VLATKPI+NY+RSP+MG+T++FSI F TP+ KI  +KE+I  
Sbjct: 602 LTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAE 661

BLAST of PI0007420 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 478.4 bits (1230), Expect = 9.1e-135
Identity = 314/744 (42.20%), Postives = 423/744 (56.85%), Query Frame = 0

Query: 14  SQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDASPLAP-------- 73
           +++   NG EVV+ +S  E+    P+ +   +P   P  S  G   + P+ P        
Sbjct: 2   AERRVSNGEEVVINVSDKEDSKD-PRASPSFNPLASP-DSDAGIEKSKPVPPISIPTPEI 61

Query: 74  -----TANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGY-IDSDMFEEENHKLKS 133
                + +KPPKIP   G L  R+SL RS  SKPKSRFGEQ  +  DS   E     L+ 
Sbjct: 62  YKFSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLRE 121

Query: 134 QHAESPLREKMMGMCGLNKK------------------------HKKVKVKTV------- 193
           Q                N K                        +KKVK+  V       
Sbjct: 122 QFGAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMKP 181

Query: 194 --FKWIGVF-CIISCLVASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVV 253
             F  + VF  I+  L+ SLT++ +    +WGL+ WKWC+L  V L G++ T W M+ VV
Sbjct: 182 LAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVV 241

Query: 254 FLIERNFLLK----------KKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDA 313
           F+IE+N+LL+          KK+VQV +W SLVL  W  LFD     +  +R T + LD 
Sbjct: 242 FIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGD---VKRTRKTKRFLDF 301

Query: 314 VTWTLASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQECLFLHHVLQTLLRPLPVED- 373
           +TWT+ SLL+G+ L+L+KT  LK+LASKF++  FF+RIQE +F  +VLQTL  P  +E+ 
Sbjct: 302 ITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEA 361

Query: 374 ------ESTAKFRCCRF---CFESKK----------SDQKVSAWTMKVLVDAV-TSSEMS 433
                  ST      R      + KK            +KVSAWTM+VL++AV TS   +
Sbjct: 362 ENVGRVPSTGHLSFTRTKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSGIST 421

Query: 434 ISQILDD-ESYRDVADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVK 493
           IS  LD+  + ++  D EITNEM+ A  AA ++F NVA P + +IEE DL  FMI EEV 
Sbjct: 422 ISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKEEVD 481

Query: 494 LVWPHFE----------------MKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVT- 553
           LV P  E                + VY  RKT+ H+L DTKTAVKQL+ LIT ++ ++T 
Sbjct: 482 LVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTVITF 541

Query: 554 -----ALAVA---------------AFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGV 613
                 L +A               AFM G+TCK  FE+ +FVFVMHP+DVGDRCVVDGV
Sbjct: 542 IVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVVDGV 601

Query: 614 QLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKI 641
            LLVEE+++LTTVFLK++NEKV+YPNSVL +KPI+N+YRSPDMGD + F I F TP EKI
Sbjct: 602 MLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPAEKI 661

BLAST of PI0007420 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 357.1 bits (915), Expect = 3.0e-98
Identity = 254/731 (34.75%), Postives = 372/731 (50.89%), Query Frame = 0

Query: 10  RRSSSQKESENGGEVVVQLSGVENEYSVPKQNRVDSPTKEPTGSSVGYGDASPLAPTANK 69
           RR S    + N    VV+ SG     + P Q    +  K  T S +    + P  PT   
Sbjct: 136 RRDSRSNTNNNDDGEVVKCSG----NNAPIQRSSSTLLKMRTRSRL----SDP--PTPQL 195

Query: 70  PPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDMFEEENHKLKSQHAESPLREK 129
           PP+         P+    +S        FG+ P    +   EEE+    ++      R+ 
Sbjct: 196 PPQTADMKSGRIPKSGQMKSGF------FGKSP---KTQGEEEEDDPFAAEDLPEEYRK- 255

Query: 130 MMGMCGLNKKHKKVKVKTVFKWIGVFCIISCLVASLTVNPLKNRFLWGLKVWKWCLLATV 189
                       K+ +  V +W+ +  II+  V +L +  L+ + LW L++WKW  +  V
Sbjct: 256 -----------DKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLV 315

Query: 190 ILCGLIFTRWVMNVVVFLIERNFLLKK----------KSVQVTLWLSLVLATWGSLFDRR 249
           ++CG + + W++ +VVF IERNFLL+K          K+VQ  LWL LVL  W  LFD +
Sbjct: 316 LICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEK 375

Query: 250 NHMISSSRITAKILDAVTWTLASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQECLFL 309
                +     K L  VT     LL+G  LWL+KTLL+K+LAS FHM+ +FDRIQE LF 
Sbjct: 376 ----VAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFT 435

Query: 310 HHVLQTLLRPLPVEDESTAKFRCCRFCFESKK---------------------------- 369
            +V++TL  P  +E +   +    R   E KK                            
Sbjct: 436 QYVIETLSGPPLIEIQKNEEEE-ERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSH 495

Query: 370 -----------------------SDQKVSAWTMKVLVDAVTSSEM-SISQILDDESYRDV 429
                                  + + VSAW MK L++ + +  + ++ + L D S  D 
Sbjct: 496 VLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDD 555

Query: 430 ADDEITNEMKVAKEAAKEIFKNVALPGNKFIEERDLQVFMIAEEVKLVWPHFE------- 489
             ++I +E + AK AA++IF NVA PG+KFI   D+  F+  +E       FE       
Sbjct: 556 KGNQIRSEFE-AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNR 615

Query: 490 ----------MKVYQGRKTLAHALKDTKTAVKQLN---NLITALIIIV------------ 549
                     +  ++ R+ LA  L DTKTAV +L+   N++  +II+V            
Sbjct: 616 ISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTK 675

Query: 550 ------TALAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTV 609
                 + + V AF+FGN CK  FE++I++FV+HPFDVGDRC +DGVQ++VEEMNILTTV
Sbjct: 676 FLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTV 735

Query: 610 FLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIQFSIGFGTPLEKIGAMKEQIKRYLEE 641
           FL+ +N+KV YPNS+L TK I NYYRSPDMGD I+FSI   TP EKI  +K++I  Y+E 
Sbjct: 736 FLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEG 795

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYG91.9e-14544.90Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10... [more]
Q84M971.3e-13342.20Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 P... [more]
Q9SYM14.3e-9734.75Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Q9LPG36.8e-9535.02Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
Q9LH741.2e-9434.10Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
Match NameE-valueIdentityDescription
A0A1S4DZ480.0e+0083.63Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=... [more]
A0A5D3D9910.0e+0083.63Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0L5E60.0e+0081.97Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_4G647440 ... [more]
A0A5D3D9191.8e-25570.59Mechanosensitive ion channel protein 10-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1J4915.4e-25267.31Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111481199... [more]
Match NameE-valueIdentityDescription
XP_008452779.10.0e+0083.63PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_01690... [more]
KAE8649918.10.0e+0081.97hypothetical protein Csa_012502 [Cucumis sativus][more]
XP_004141433.20.0e+0081.97mechanosensitive ion channel protein 10 [Cucumis sativus][more]
XP_038899727.12.4e-27072.25mechanosensitive ion channel protein 10-like isoform X1 [Benincasa hispida] >XP_... [more]
XP_038899729.16.9e-27072.31mechanosensitive ion channel protein 10-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G12080.11.3e-14644.90mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.21.3e-14644.90mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.31.3e-14644.90mechanosensitive channel of small conductance-like 10 [more]
AT5G19520.19.1e-13542.20mechanosensitive channel of small conductance-like 9 [more]
AT1G78610.13.0e-9834.75mechanosensitive channel of small conductance-like 6 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 484..533
e-value: 1.2E-11
score: 46.3
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 442..630
e-value: 1.1E-19
score: 70.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..54
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 72..87
NoneNo IPR availablePANTHERPTHR31618:SF7MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 20..640
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 20..640
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 473..533

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0007420.1PI0007420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0050982 detection of mechanical stimulus
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008381 mechanosensitive ion channel activity