PI0007055 (gene) Melon (PI 482460) v1

Overview
NamePI0007055
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptiontolB protein-related
Locationchr01: 25650222 .. 25652170 (+)
RNA-Seq ExpressionPI0007055
SyntenyPI0007055
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAACCCTACCGGCGCCGTCCTCTTCACCACCATTGGCCTCAACCAATACGGTTTCGATATCTTCTCTGTTCCACTCAATTCCCCAACCGTCGAACGCCGACTCACCGATGGAATCTCTGTCAATTTCAACGCCCAATTCCTTAATAACCAACTTTCTGTTGTCTTCATCTCTGAACGCTCCGGCTCTTCTCGAATTTACCTCTCCGATTCCCCTAATTCTTCCCCTAAATTGCTCGCTTCCGCCCCCAGAAGCTGCTTTCATGACCGACCCATCGTTAGAAATGACCGACTTTTTTTCATCTCTGCTCATGAAAACCCCATAAGCCCTTCACCAGCTGGGCTGCTCTGTATTCCACTCCGTTGGACGGCGAAGATTCGGTTACCCGGCTGACTCCTCTCGGCTCCGTCGATTTCAGTCCAGCGGTTTCCGAAAGTGGGAAGTTTGTGGCGGTTGCTTCTTATGGCTCTCGTTCTTGGGGCGGCGAATTTCATGAGCTAAACACAGAAATTGTTGTGTTTAAATCTTCTGACCCGGATCGGAGGGTTGTGGTTGCCGGTCGGGGTGGATGGCCGTCGTGGTCCGGCGACTCCACGGTCTTCTTCCACCGGAAGGCCGACGATGGGTGGTGGAGCATTTTCAAAGTGGAGATTCCTGAAAATCTTGATTCCTATGTTTCCCCTGTTCCAATCCGGGTCACTCCGGCGGGTCTTCATTGCTTTACTCCGGCTTCCATGAACGACAGCAGACGAGTTGTCGTCGCTACACGAAGAGCCGACAGTAAATTCCAACACAGTAAATTCCGACACATCGAGATTTTCGATTCTGTATTGGAAGAATTCATCCCAATTACCCAGAAATTGAATCCCGATTTCCATCACTACAATCCGTTTGTCTCACCGGATTCCAATTTCATCGGGTACCACCGATTTCGCGGCGAGTCAGCTCAGGGCGAATTGATTCCATATCTCAAGCCGGTAATTTCCCCAATTAAAGAACTCCAAATGATCCGAATAAACGGTTCGTTCCCAACGCCGTCACCTGACGGCGATTTAATCGCGTTTAATCCCGATTTCAATGGATTACAAATCGTTAAATTTGACGGCTCGAAATGTCGAACCGTTTTAAAAGATAGAACCGCTTTCTACAATTCGTGGAGCCCAACGGAGAAAAACGTGATTTACACTTCGTTAGGACCCATTTTCGGGGCGGTAAGAGCGACGGTCCAGATCGCTCGGATCACGATCAACTCTGATGATCTCATCAGTGGTGATAGCGATGAGGTTGCCAGTGAAGTAAAGATTCTTACAAAAGACGATACCGGAAATAATGCCTTTCCGGCGTGCTCACCAGACGGTAAGTTTCTGGTCTTCCGGTCCGGGCGATCAGGACACAAGAATCTTTACATCGTCGACGCCGCGAACGGCGAGTTTGACGGCGAATTACGACGGTTGACCGATGGACCGTGGATCGACACGATGCCTAACTGGTCGCCGAGAGGAGATCTCATAGTATTTTCTTCAAATATGCATAATCCAAAGAACACTGAAGCATTTAGTATCTACGTCATCAGACCGGACGGTTCGGGGTTGAGGAGAGTATACGTGGCTGGACCGGAAGGGTCTAGTGACGTGGATAGAGAGAGGATTAATCACGTGTGTTTTAGTAGGGACGGTAAATGGCTTTTATTTACGGCAAATTTGAGTGGGGTGACAGCGGAGCCGGTGTCATTACCCAATCAGTTTCAGCCATATGGCGATTTGTTTGTGGTGCGATTGGATGGGACCGGGTTGAGGAGGCTGACGTGGAATGCATATGAAAATGGGACTCCCACGTGGTATTACGGGAGTGAGCTGGCACTTTCTGGTTTGAGTTTGAAAGATGAGGTGGTTGGTCAAAAGTTGAAAGGAGAATTTGATGAACCGCTTTGGATAACGTTTGACTGA

mRNA sequence

ATGGATAACCCTACCGGCGCCGTCCTCTTCACCACCATTGGCCTCAACCAATACGGTTTCGATATCTTCTCTGTTCCACTCAATTCCCCAACCGTCGAACGCCGACTCACCGATGGAATCTCTGTCAATTTCAACGCCCAATTCCTTAATAACCAACTTTCTGTTGTCTTCATCTCTGAACGCTCCGGCTCTTCTCGAATTTACCTCTCCGATTCCCCTAATTCTTCCCCTAAATTGCTCGCTTCCGCCCCCAGAAGCTGCTTTCATGACCGACCCATCCCCTTCACCAGCTGGGCTGCTCTGTATTCCACTCCGTTGGACGGCGAAGATTCGGTTACCCGGCTGACTCCTCTCGGCTCCGTCGATTTCAGTCCAGCGGTTTCCGAAAGTGGGAAGTTTGTGGCGGTTGCTTCTTATGGCTCTCGTTCTTGGGGCGGCGAATTTCATGAGCTAAACACAGAAATTGTTGTGTTTAAATCTTCTGACCCGGATCGGAGGGTTGTGGTTGCCGGTCGGGGTGGATGGCCGTCGTGGTCCGGCGACTCCACGGTCTTCTTCCACCGGAAGGCCGACGATGGGTGGTGGAGCATTTTCAAAGTGGAGATTCCTGAAAATCTTGATTCCTATGTTTCCCCTGTTCCAATCCGGGTCACTCCGGCGGGTCTTCATTGCTTTACTCCGGCTTCCATGAACGACAGCAGACGAGTTGTCGTCGCTACACGAAGAGCCGACAGTAAATTCCAACACAGTAAATTCCGACACATCGAGATTTTCGATTCTGTATTGGAAGAATTCATCCCAATTACCCAGAAATTGAATCCCGATTTCCATCACTACAATCCGTTTGTCTCACCGGATTCCAATTTCATCGGGTACCACCGATTTCGCGGCGAGTCAGCTCAGGGCGAATTGATTCCATATCTCAAGCCGGTAATTTCCCCAATTAAAGAACTCCAAATGATCCGAATAAACGGTTCGTTCCCAACGCCGTCACCTGACGGCGATTTAATCGCGTTTAATCCCGATTTCAATGGATTACAAATCGTTAAATTTGACGGCTCGAAATGTCGAACCGTTTTAAAAGATAGAACCGCTTTCTACAATTCGTGGAGCCCAACGGAGAAAAACGTGATTTACACTTCGTTAGGACCCATTTTCGGGGCGGTAAGAGCGACGGTCCAGATCGCTCGGATCACGATCAACTCTGATGATCTCATCAGTGGTGATAGCGATGAGGTTGCCAGTGAAGTAAAGATTCTTACAAAAGACGATACCGGAAATAATGCCTTTCCGGCGTGCTCACCAGACGGTAAGTTTCTGGTCTTCCGGTCCGGGCGATCAGGACACAAGAATCTTTACATCGTCGACGCCGCGAACGGCGAGTTTGACGGCGAATTACGACGGTTGACCGATGGACCGTGGATCGACACGATGCCTAACTGGTCGCCGAGAGGAGATCTCATAGTATTTTCTTCAAATATGCATAATCCAAAGAACACTGAAGCATTTAGTATCTACGTCATCAGACCGGACGGTTCGGGGTTGAGGAGAGTATACGTGGCTGGACCGGAAGGGTCTAGTGACGTGGATAGAGAGAGGATTAATCACGTGTGTTTTAGTAGGGACGGTAAATGGCTTTTATTTACGGCAAATTTGAGTGGGGTGACAGCGGAGCCGGTGTCATTACCCAATCAGTTTCAGCCATATGGCGATTTGTTTGTGGTGCGATTGGATGGGACCGGGTTGAGGAGGCTGACGTGGAATGCATATGAAAATGGGACTCCCACGTGGTATTACGGGAGTGAGCTGGCACTTTCTGGTTTGAGTTTGAAAGATGAGGTGGTTGGTCAAAAGTTGAAAGGAGAATTTGATGAACCGCTTTGGATAACGTTTGACTGA

Coding sequence (CDS)

ATGGATAACCCTACCGGCGCCGTCCTCTTCACCACCATTGGCCTCAACCAATACGGTTTCGATATCTTCTCTGTTCCACTCAATTCCCCAACCGTCGAACGCCGACTCACCGATGGAATCTCTGTCAATTTCAACGCCCAATTCCTTAATAACCAACTTTCTGTTGTCTTCATCTCTGAACGCTCCGGCTCTTCTCGAATTTACCTCTCCGATTCCCCTAATTCTTCCCCTAAATTGCTCGCTTCCGCCCCCAGAAGCTGCTTTCATGACCGACCCATCCCCTTCACCAGCTGGGCTGCTCTGTATTCCACTCCGTTGGACGGCGAAGATTCGGTTACCCGGCTGACTCCTCTCGGCTCCGTCGATTTCAGTCCAGCGGTTTCCGAAAGTGGGAAGTTTGTGGCGGTTGCTTCTTATGGCTCTCGTTCTTGGGGCGGCGAATTTCATGAGCTAAACACAGAAATTGTTGTGTTTAAATCTTCTGACCCGGATCGGAGGGTTGTGGTTGCCGGTCGGGGTGGATGGCCGTCGTGGTCCGGCGACTCCACGGTCTTCTTCCACCGGAAGGCCGACGATGGGTGGTGGAGCATTTTCAAAGTGGAGATTCCTGAAAATCTTGATTCCTATGTTTCCCCTGTTCCAATCCGGGTCACTCCGGCGGGTCTTCATTGCTTTACTCCGGCTTCCATGAACGACAGCAGACGAGTTGTCGTCGCTACACGAAGAGCCGACAGTAAATTCCAACACAGTAAATTCCGACACATCGAGATTTTCGATTCTGTATTGGAAGAATTCATCCCAATTACCCAGAAATTGAATCCCGATTTCCATCACTACAATCCGTTTGTCTCACCGGATTCCAATTTCATCGGGTACCACCGATTTCGCGGCGAGTCAGCTCAGGGCGAATTGATTCCATATCTCAAGCCGGTAATTTCCCCAATTAAAGAACTCCAAATGATCCGAATAAACGGTTCGTTCCCAACGCCGTCACCTGACGGCGATTTAATCGCGTTTAATCCCGATTTCAATGGATTACAAATCGTTAAATTTGACGGCTCGAAATGTCGAACCGTTTTAAAAGATAGAACCGCTTTCTACAATTCGTGGAGCCCAACGGAGAAAAACGTGATTTACACTTCGTTAGGACCCATTTTCGGGGCGGTAAGAGCGACGGTCCAGATCGCTCGGATCACGATCAACTCTGATGATCTCATCAGTGGTGATAGCGATGAGGTTGCCAGTGAAGTAAAGATTCTTACAAAAGACGATACCGGAAATAATGCCTTTCCGGCGTGCTCACCAGACGGTAAGTTTCTGGTCTTCCGGTCCGGGCGATCAGGACACAAGAATCTTTACATCGTCGACGCCGCGAACGGCGAGTTTGACGGCGAATTACGACGGTTGACCGATGGACCGTGGATCGACACGATGCCTAACTGGTCGCCGAGAGGAGATCTCATAGTATTTTCTTCAAATATGCATAATCCAAAGAACACTGAAGCATTTAGTATCTACGTCATCAGACCGGACGGTTCGGGGTTGAGGAGAGTATACGTGGCTGGACCGGAAGGGTCTAGTGACGTGGATAGAGAGAGGATTAATCACGTGTGTTTTAGTAGGGACGGTAAATGGCTTTTATTTACGGCAAATTTGAGTGGGGTGACAGCGGAGCCGGTGTCATTACCCAATCAGTTTCAGCCATATGGCGATTTGTTTGTGGTGCGATTGGATGGGACCGGGTTGAGGAGGCTGACGTGGAATGCATATGAAAATGGGACTCCCACGTGGTATTACGGGAGTGAGCTGGCACTTTCTGGTTTGAGTTTGAAAGATGAGGTGGTTGGTCAAAAGTTGAAAGGAGAATTTGATGAACCGCTTTGGATAACGTTTGACTGA

Protein sequence

MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISERSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPIPFTSWAALYSTPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDPDRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYVSPVPIRVTPAGLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYNPFVSPDSNFIGYHRFRGESAQGELIPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFNPDFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITINSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANGEFDGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD
Homology
BLAST of PI0007055 vs. ExPASy Swiss-Prot
Match: Q39PS9 (Tol-Pal system protein TolB OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15) OX=269799 GN=tolB PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 6.2e-16
Identity = 79/277 (28.52%), Postives = 127/277 (45.85%), Query Frame = 0

Query: 348 IVKFDGSKCRTVLKDRTAFYN-SWSPTEKNVIYTSL----GPIFGAVRATVQIARIT--- 407
           ++ +DG   + + K+R+   N  +SP+ K +I+TS       +F     T   ARI+   
Sbjct: 184 LMDYDGHNVQQLTKNRSINLNPDFSPSGKEIIFTSYRQGNPDLFRRELFTGAEARISSYR 243

Query: 408 -INSDDLISGDSDEVA--------SEVKILTKDD------TGNNAF---PACSPDGKFLV 467
            IN+    S D   +A        SE+  +++D       T  +A    PA SPD + +V
Sbjct: 244 GINATGAWSPDGKTIALTLSKDGNSEIYTISRDGKNPHRLTNTSAIEVSPAWSPDVRKIV 303

Query: 468 FRSGRSGHKNLYIVDAANGEFDGE-LRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNT 527
           F S R G   ++I++A     DG  +RRLT     +  P WSP+GD IV+S      +  
Sbjct: 304 FVSDRLGKPQIFIMNA-----DGTGVRRLTTSGNYNVSPRWSPKGDRIVYS------RQE 363

Query: 528 EAFSIYVIRPDGSGLRRVYVAGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPV 587
             F IY I PDG+   R+     EGS++       H  +S DG+++ F++   G      
Sbjct: 364 GGFQIYAINPDGTNDTRL---TSEGSNE-------HPRWSPDGRFITFSSTRGGGEG--- 423

Query: 588 SLPNQFQPYGDLFVVRLDGTGLRRLTWNAYENGTPTW 598
                      ++V+R DG+G  R++     +  PTW
Sbjct: 424 -----------IYVMRADGSGQTRVSGGKGRDSHPTW 425

BLAST of PI0007055 vs. ExPASy Swiss-Prot
Match: Q74H67 (Tol-Pal system protein TolB OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=tolB PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 5.2e-15
Identity = 79/280 (28.21%), Postives = 125/280 (44.64%), Query Frame = 0

Query: 346 LQIVKFDGSKCRTVLKDRTAFYN-SWSPTEKNVIYTSL---------GPIFGAVRATVQI 405
           + ++ +DG   + + K+R+   N  +SP  + + YTS            IF    A V  
Sbjct: 185 IYLMDYDGHNVQRLTKNRSINLNPDFSPNGRELAYTSYRRGNPDLFRREIFTGTEAVVSS 244

Query: 406 ARITINSDDLISGDSDEVA--------SEVKILTKDD------TGNNAF---PACSPDGK 465
            R  IN     S D  ++A        SE+  +++D       T + A    PA SPDGK
Sbjct: 245 HR-GINVTGTWSPDGKQLALAMSRDGNSEIYAISRDGRDPRRLTTHQAIDVSPAWSPDGK 304

Query: 466 FLVFRSGRSGHKNLYIVDAANGEFDG-ELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNP 525
            + F S R G   ++I++A     DG ++RRLT     +  P WSP+GD +V+       
Sbjct: 305 RIAFVSDRLGKPQVFIMNA-----DGSDVRRLTTSGAYNVSPRWSPKGDRLVYC------ 364

Query: 526 KNTEAFSIYVIRPDGSGLRRVYVAGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTA 585
           +    F IY I  DG+G  R+     EGS++       H  +S DG++L F++   G  A
Sbjct: 365 RQEGGFQIYSIATDGTGDTRL---TSEGSNE-------HPRWSPDGRFLTFSSTRDGGEA 424

Query: 586 EPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNAYENGTPTW 598
                         ++V+R DG+G  R+  +  +   PTW
Sbjct: 425 --------------IYVMRSDGSGQTRVYRSKGKASHPTW 428

BLAST of PI0007055 vs. ExPASy Swiss-Prot
Match: A8LHQ6 (Tol-Pal system protein TolB OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) OX=398580 GN=tolB PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 7.6e-14
Identity = 55/155 (35.48%), Postives = 76/155 (49.03%), Query Frame = 0

Query: 431 PACSPDGKFLVFRSGRSGHKNLYIVDAANGEFDGELRRLTDGPWIDTMPNWSPRGDLIVF 490
           P+ SPDG+ +VF S RSG + LY++ A      GE RR++ GP     P WSPRGDLI F
Sbjct: 306 PSFSPDGRQIVFESDRSGSQQLYVMSATG----GEARRISFGPGRYGTPVWSPRGDLIAF 365

Query: 491 SSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGPEGSSDVDRERINHVCFSRDGKWLLFTA 550
           +      +N   F I V+R DGS  R +       SS +D        +S +G+ ++FT 
Sbjct: 366 TK-----QNQGRFHIGVMRTDGSEERLL------TSSFLDES----PTWSPNGRVIMFTR 425

Query: 551 NLSGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRL 586
             SG    P            L+ V + G  LRR+
Sbjct: 426 ETSGAGGAP-----------SLYSVDISGRNLRRV 430

BLAST of PI0007055 vs. ExPASy Swiss-Prot
Match: Q3APB5 (Protein TolB homolog OS=Chlorobium chlorochromatii (strain CaD3) OX=340177 GN=tolB PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.2e-13
Identity = 55/184 (29.89%), Postives = 83/184 (45.11%), Query Frame = 0

Query: 414 ASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANGEFDGELRRLTDGP 473
           A  VK      +G +  P  SPDG+ + F S RSG+  +++ D ++    G+ +RLT   
Sbjct: 283 AGAVKQRLTSSSGIDLSPTFSPDGRKMAFVSARSGNPQIFVYDFSS----GKSQRLTFSG 342

Query: 474 WIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGPEGSSDVDRER 533
             +T P WSP GD I FS+     ++    +I+VI  DGSGL ++     E  S      
Sbjct: 343 RYNTQPAWSPIGDKIAFST----WESGGEINIFVINTDGSGLTQLTTQSGENESP----- 402

Query: 534 INHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGTGLRRLTWNAYENG 593
                +S DG+ ++F +N  GV                L+V+  DG   R L     E  
Sbjct: 403 ----SWSPDGRMIVFASNRQGVK--------------KLYVMMADGKNQRPLLAIGGEQT 435

Query: 594 TPTW 598
            P+W
Sbjct: 463 QPSW 435

BLAST of PI0007055 vs. ExPASy Swiss-Prot
Match: Q167Z6 (Tol-Pal system protein TolB OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) OX=375451 GN=tolB PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 6.4e-13
Identity = 52/139 (37.41%), Postives = 75/139 (53.96%), Query Frame = 0

Query: 431 PACSPDGKFLVFRSGRSGHKNLYIVDAANGEFDGELRRLTDGPWIDTMPNWSPRGDLIVF 490
           P+ SPDG  +VF S RSG + LY++  ANG   GE RR++ GP     P WSPRGDL+ F
Sbjct: 303 PSYSPDGTQIVFESDRSGSQQLYVM-PANG---GEARRISFGPGRYGTPVWSPRGDLVAF 362

Query: 491 SSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAG-PEGSSDVDRERINHVCFSRD-----GK 550
           +      +N   F I V+R DGS  R +  +   EG +     R+  + FSR+     G+
Sbjct: 363 TK-----QNAGRFHIGVMRLDGSEERLLTASFLDEGPTWSPNGRV--IMFSRETQGAQGR 422

Query: 551 WLLFTANLSGVTAEPVSLP 564
             L++ +++G    PV  P
Sbjct: 423 ATLYSVDITGRNLRPVRTP 430

BLAST of PI0007055 vs. ExPASy TrEMBL
Match: A0A5A7URS2 (TolB protein-related isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00020 PE=4 SV=1)

HSP 1 Score: 1221.8 bits (3160), Expect = 0.0e+00
Identity = 597/652 (91.56%), Postives = 608/652 (93.25%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           MDNPTGAVLFTTIGLNQYGFDIFSV LNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE
Sbjct: 1   MDNPTGAVLFTTIGLNQYGFDIFSVALNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGSSRIYLSDSPNSSPKLLASAP SCFHDRPI                 PFTSWAALYS
Sbjct: 61  RSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVRNGRLLFISAHENPHKPFTSWAALYS 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           TPLDG+DS+TRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELN EIVVFKSSDP
Sbjct: 121 TPLDGDDSITRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYV---SPVPIRVTPA 240
           DRRVVVA RGGWPSWSGDSTVFFHRKA+DGWWSIFKVEIPENLDS +   SPVPIRVTPA
Sbjct: 181 DRRVVVASRGGWPSWSGDSTVFFHRKAEDGWWSIFKVEIPENLDSSMSSDSPVPIRVTPA 240

Query: 241 GLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYN 300
           GLHCFTPA+MNDSR VVVATRRAD     SKFRHIEIFDS LEEFIPITQKLNPDFHHYN
Sbjct: 241 GLHCFTPAAMNDSRLVVVATRRAD-----SKFRHIEIFDSELEEFIPITQKLNPDFHHYN 300

Query: 301 PFVSPDSNFIGYHRFRGESAQGELIPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFN 360
           PFVSPDSNFIGYHRFRGES Q ELIPYL PVISPIKELQMIRINGSFPTPSPDGDLIAFN
Sbjct: 301 PFVSPDSNFIGYHRFRGESTQSELIPYLSPVISPIKELQMIRINGSFPTPSPDGDLIAFN 360

Query: 361 PDFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITI 420
           P+FNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAV ATVQIARITI
Sbjct: 361 PNFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVTATVQIARITI 420

Query: 421 NSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANG 480
           NSDDL  GDSDEV+SEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDA  G
Sbjct: 421 NSDDLKDGDSDEVSSEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVKG 480

Query: 481 EFDGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540
           EF+GELRRLTDG WIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV
Sbjct: 481 EFEGELRRLTDGAWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540

Query: 541 AGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT 600
           AGPEGSS+VDRERINHVCFSRDGKWLLFTANLSGVTAEPVS PNQFQPYGDLFVVRLDGT
Sbjct: 541 AGPEGSSEVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSWPNQFQPYGDLFVVRLDGT 600

Query: 601 GLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           GLRRLTWN YENGTPTWYYGSE+ALSGLSLKDEVVG+KLKG FDEPLWITFD
Sbjct: 601 GLRRLTWNGYENGTPTWYYGSEVALSGLSLKDEVVGEKLKGNFDEPLWITFD 647

BLAST of PI0007055 vs. ExPASy TrEMBL
Match: A0A1S3CEJ8 (uncharacterized protein LOC103499982 OS=Cucumis melo OX=3656 GN=LOC103499982 PE=4 SV=1)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 594/652 (91.10%), Postives = 605/652 (92.79%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           MDNPTGAVLFTTIGLNQYGFDIFSV LNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE
Sbjct: 1   MDNPTGAVLFTTIGLNQYGFDIFSVALNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGSSRIYLSDSPNSSPKLLASAP SCFHDRPI                 PFTSWAALYS
Sbjct: 61  RSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVRNGRLLFISAHENPHKPFTSWAALYS 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           TPLDG+DS+TRLTPLGSVDFSPAVS SGKFVAVASYGSRSWGGEFHELN EIVVFKSSDP
Sbjct: 121 TPLDGDDSITRLTPLGSVDFSPAVSGSGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYV---SPVPIRVTPA 240
           DRRVVVA RGGWPSWSGDSTVFFHRKA+DGWWSIFKVEIPENLDS +   SPVPIRVTPA
Sbjct: 181 DRRVVVASRGGWPSWSGDSTVFFHRKAEDGWWSIFKVEIPENLDSSMSSDSPVPIRVTPA 240

Query: 241 GLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYN 300
           GLHCFTPA+MNDSR VVVATRRAD     SKFRHIEIFDS LEEFIPITQKLNPDFHHYN
Sbjct: 241 GLHCFTPAAMNDSRLVVVATRRAD-----SKFRHIEIFDSELEEFIPITQKLNPDFHHYN 300

Query: 301 PFVSPDSNFIGYHRFRGESAQGELIPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFN 360
           PFVSPDSNFIGYHRFRGES Q ELIPYL PVISPIKELQMIRINGSFP PSPDGDLIAFN
Sbjct: 301 PFVSPDSNFIGYHRFRGESTQSELIPYLSPVISPIKELQMIRINGSFPAPSPDGDLIAFN 360

Query: 361 PDFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITI 420
           P+FNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAV ATVQIARITI
Sbjct: 361 PNFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVTATVQIARITI 420

Query: 421 NSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANG 480
           NSDDL  GDSDEV+SEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDA  G
Sbjct: 421 NSDDLKDGDSDEVSSEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVKG 480

Query: 481 EFDGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540
           EF+GELRRLTDG WIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV
Sbjct: 481 EFEGELRRLTDGAWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540

Query: 541 AGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT 600
           AGPEGSS+VDRERINHVCFSRDGKWLLFTANLSGVTAEPVS PNQFQPYGDLFVVRLDGT
Sbjct: 541 AGPEGSSEVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSWPNQFQPYGDLFVVRLDGT 600

Query: 601 GLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           GL RLTWN YENGTPTWYYGSE+ALSGLSLKDEVVG+KLKG FDEPLWITFD
Sbjct: 601 GLSRLTWNGYENGTPTWYYGSEVALSGLSLKDEVVGEKLKGNFDEPLWITFD 647

BLAST of PI0007055 vs. ExPASy TrEMBL
Match: A0A0A0K6G2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G419590 PE=4 SV=1)

HSP 1 Score: 1187.2 bits (3070), Expect = 0.0e+00
Identity = 584/652 (89.57%), Postives = 601/652 (92.18%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           MDNPTGAVLFTT+GLNQYGFDIFSVPLNS TVER+LTDGISVNFNAQFLNNQLSVVFISE
Sbjct: 1   MDNPTGAVLFTTVGLNQYGFDIFSVPLNSLTVERQLTDGISVNFNAQFLNNQLSVVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGSSRIYLSDSPNSSPKLLASAP SCFHDRPI                 PFTSWAALYS
Sbjct: 61  RSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVTNGRLLFISAHENPHKPFTSWAALYS 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           TPLDG DSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELN EIVVFKSSDP
Sbjct: 121 TPLDGHDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLD---SYVSPVPIRVTPA 240
            +RVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLD   S VSPV IRVTPA
Sbjct: 181 GQRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSSRSSVSPVAIRVTPA 240

Query: 241 GLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYN 300
           GLHCFTPA+MND RRVVVATRRAD     SK+RHIEIFDS LEEFIPITQKLNP+FHHYN
Sbjct: 241 GLHCFTPAAMNDGRRVVVATRRAD-----SKYRHIEIFDSELEEFIPITQKLNPEFHHYN 300

Query: 301 PFVSPDSNFIGYHRFRGESAQGELIPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFN 360
           PFVSPDSNFIGYHRFRGES Q ELIPYL PVISPIKELQ+IR+NGSFPTPSPDGDLIAFN
Sbjct: 301 PFVSPDSNFIGYHRFRGESTQSELIPYLYPVISPIKELQIIRVNGSFPTPSPDGDLIAFN 360

Query: 361 PDFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITI 420
           P F GLQIVKFDGSKCRTVLKDRTAF NSWSPTEKNVIYTSLGPIFGAV ATVQIARITI
Sbjct: 361 PGFIGLQIVKFDGSKCRTVLKDRTAFCNSWSPTEKNVIYTSLGPIFGAVTATVQIARITI 420

Query: 421 NSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANG 480
           N     SGDSDEV++EVKILTKD+TGNNAFPACSPDGKFLVFRSGR+GHKNLYIVDA  G
Sbjct: 421 N-----SGDSDEVSNEVKILTKDNTGNNAFPACSPDGKFLVFRSGRTGHKNLYIVDAMKG 480

Query: 481 EFDGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540
           EF+GELR+LTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV
Sbjct: 481 EFEGELRQLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540

Query: 541 AGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT 600
           AGPEGSS+VDRERINHVCFSRDG WLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT
Sbjct: 541 AGPEGSSEVDRERINHVCFSRDGNWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT 600

Query: 601 GLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           GLRRLT NAYENGTPTWYYGSELALSGLSLKDEVVG+KLKG+FDEPLWI FD
Sbjct: 601 GLRRLTCNAYENGTPTWYYGSELALSGLSLKDEVVGEKLKGDFDEPLWIKFD 642

BLAST of PI0007055 vs. ExPASy TrEMBL
Match: A0A6J1GRK1 (uncharacterized protein LOC111456480 OS=Cucurbita moschata OX=3662 GN=LOC111456480 PE=4 SV=1)

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 531/650 (81.69%), Postives = 580/650 (89.23%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           M NPTG V+FTT+G  QYGFD FSVPL+SPT E  LTDGISVNFNAQF++NQ S+VFISE
Sbjct: 1   MTNPTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGS R+YLS+SPNS+PKLL SAP SCFHDRPI                 PFTSW+ALY 
Sbjct: 61  RSGSPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYF 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           T LDG DSVTRLTP GSVDFSPAVSESGKFVAVASYGSRSWGGEF EL+TEIVVF+SSDP
Sbjct: 121 TGLDGSDSVTRLTPCGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYVSPVPIRVTPAGLH 240
           DRRVVV+GRGGWPSWSGDSTV+FHR+A+DGWWSIF+VEIPENLDS V PVPIRVTPAGLH
Sbjct: 181 DRRVVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVPIRVTPAGLH 240

Query: 241 CFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYNPFV 300
           CFTPA+MND +RVVVATRR D     +KFRHIEI++S  +EF PITQKLNP FHHYNPFV
Sbjct: 241 CFTPAAMNDRKRVVVATRRPD-----NKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFV 300

Query: 301 SPDSNFIGYHRFRGESAQGEL-IPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFNPD 360
           SPDS +IGYHRFRGES+ GEL IP+ + +ISPI EL++IRINGSFPT SPDG+ IAFNPD
Sbjct: 301 SPDSKYIGYHRFRGESSHGELTIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPD 360

Query: 361 FNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITINS 420
           F GL+IVK DGSKC TVLKDRTAFYNSWSPTEKNVIY+SLGPIFG  RATVQIAR TINS
Sbjct: 361 FVGLKIVKADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINS 420

Query: 421 DDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANGEF 480
           DDL +GDSDEVA EVKILTK+DTGNNAFPACSPDGKFLVFRSGRSGHKNLYI+DA NG+F
Sbjct: 421 DDLNNGDSDEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDF 480

Query: 481 DGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAG 540
           +GE RRLTDGPWIDTMP+WSP GDLI FSSNMHNP+NTE FSIYVIRPDGS LRRV+VAG
Sbjct: 481 NGEARRLTDGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAG 540

Query: 541 PEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGTGL 600
           PEGSSDVD+ERINHVCFSRDG+WLLFT+NL GV+AEPVS+PNQFQPYGDLFVVRLDGTGL
Sbjct: 541 PEGSSDVDKERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGL 600

Query: 601 RRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           RRLTW+AYENGTPTWYYGSELALSGLSLKDEVVG+KLKGEFDEPLWITF+
Sbjct: 601 RRLTWSAYENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of PI0007055 vs. ExPASy TrEMBL
Match: A0A6J1JTR3 (uncharacterized protein LOC111488250 OS=Cucurbita maxima OX=3661 GN=LOC111488250 PE=4 SV=1)

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 525/650 (80.77%), Postives = 579/650 (89.08%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           M NPTG V+FTT+G  QYGFD FSVPL+SPT E  LTDGISVNFNAQF++NQ S+VFISE
Sbjct: 1   MTNPTGTVIFTTVGRTQYGFDTFSVPLSSPTTEHCLTDGISVNFNAQFVDNQQSIVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGS R+YLS+SPNS+PKLL SAP SCFHDRPI                 PFTSW+ALY 
Sbjct: 61  RSGSPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYV 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           T LDG  SVTRLTP GSVDFSPAVSESGKFVAVASYGSRSWGGEF EL+TEIVVF+SSDP
Sbjct: 121 TGLDGSVSVTRLTPRGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYVSPVPIRVTPAGLH 240
           DRRV+V+GRGGWPSWSGDSTVFFHR+A+DGWWSIF+ EIPENLDS V PVPIRVTPAGLH
Sbjct: 181 DRRVIVSGRGGWPSWSGDSTVFFHRQAEDGWWSIFRGEIPENLDSSVPPVPIRVTPAGLH 240

Query: 241 CFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYNPFV 300
           CFTPA+MND +RVVVATRR D     +KFRHIEI++S  +EF PITQKLNP FHHYNPFV
Sbjct: 241 CFTPAAMNDRKRVVVATRRPD-----NKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFV 300

Query: 301 SPDSNFIGYHRFRGESAQGEL-IPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFNPD 360
           SPDSN+IGYHRFRGES+ GE+ IP+ + +ISPI EL++IRINGSFPT SPDG+ IAFNPD
Sbjct: 301 SPDSNYIGYHRFRGESSHGEITIPHFERIISPINELRLIRINGSFPTQSPDGNFIAFNPD 360

Query: 361 FNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITINS 420
           F GL+IVK DGSKC TVLKDRTAFYNSWSPTEKNVIY+SLGPIFG  RATVQIAR TINS
Sbjct: 361 FVGLKIVKADGSKCWTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINS 420

Query: 421 DDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANGEF 480
           DDL +GD+DEV+ EVKILTK+DTGNNAFPACSPDGKFLVFRSGRSGHKNLYI+DA NG+F
Sbjct: 421 DDLNNGDNDEVSGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDF 480

Query: 481 DGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAG 540
           +GE R+LT+GPWIDTMP+WSP GDLI FSSNMHNP+NTE FSIYVIRPDGS LRRV+VAG
Sbjct: 481 NGEARQLTNGPWIDTMPSWSPAGDLIAFSSNMHNPENTETFSIYVIRPDGSNLRRVHVAG 540

Query: 541 PEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGTGL 600
           PEGSSDVD+ERINHVCFSRDG+WLLFT+NL GV+AEPVS+PNQFQPYGDLFVVRLDGTGL
Sbjct: 541 PEGSSDVDKERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGL 600

Query: 601 RRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           RRLTW+AYENGTPTWYYGSELALSGLSLKDEVVG+KLKGEFDEPLWITF+
Sbjct: 601 RRLTWSAYENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of PI0007055 vs. NCBI nr
Match: KAA0058562.1 (TolB protein-related isoform 1 [Cucumis melo var. makuwa] >TYK10360.1 TolB protein-related isoform 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1221.8 bits (3160), Expect = 0.0e+00
Identity = 597/652 (91.56%), Postives = 608/652 (93.25%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           MDNPTGAVLFTTIGLNQYGFDIFSV LNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE
Sbjct: 1   MDNPTGAVLFTTIGLNQYGFDIFSVALNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGSSRIYLSDSPNSSPKLLASAP SCFHDRPI                 PFTSWAALYS
Sbjct: 61  RSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVRNGRLLFISAHENPHKPFTSWAALYS 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           TPLDG+DS+TRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELN EIVVFKSSDP
Sbjct: 121 TPLDGDDSITRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYV---SPVPIRVTPA 240
           DRRVVVA RGGWPSWSGDSTVFFHRKA+DGWWSIFKVEIPENLDS +   SPVPIRVTPA
Sbjct: 181 DRRVVVASRGGWPSWSGDSTVFFHRKAEDGWWSIFKVEIPENLDSSMSSDSPVPIRVTPA 240

Query: 241 GLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYN 300
           GLHCFTPA+MNDSR VVVATRRAD     SKFRHIEIFDS LEEFIPITQKLNPDFHHYN
Sbjct: 241 GLHCFTPAAMNDSRLVVVATRRAD-----SKFRHIEIFDSELEEFIPITQKLNPDFHHYN 300

Query: 301 PFVSPDSNFIGYHRFRGESAQGELIPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFN 360
           PFVSPDSNFIGYHRFRGES Q ELIPYL PVISPIKELQMIRINGSFPTPSPDGDLIAFN
Sbjct: 301 PFVSPDSNFIGYHRFRGESTQSELIPYLSPVISPIKELQMIRINGSFPTPSPDGDLIAFN 360

Query: 361 PDFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITI 420
           P+FNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAV ATVQIARITI
Sbjct: 361 PNFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVTATVQIARITI 420

Query: 421 NSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANG 480
           NSDDL  GDSDEV+SEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDA  G
Sbjct: 421 NSDDLKDGDSDEVSSEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVKG 480

Query: 481 EFDGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540
           EF+GELRRLTDG WIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV
Sbjct: 481 EFEGELRRLTDGAWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540

Query: 541 AGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT 600
           AGPEGSS+VDRERINHVCFSRDGKWLLFTANLSGVTAEPVS PNQFQPYGDLFVVRLDGT
Sbjct: 541 AGPEGSSEVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSWPNQFQPYGDLFVVRLDGT 600

Query: 601 GLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           GLRRLTWN YENGTPTWYYGSE+ALSGLSLKDEVVG+KLKG FDEPLWITFD
Sbjct: 601 GLRRLTWNGYENGTPTWYYGSEVALSGLSLKDEVVGEKLKGNFDEPLWITFD 647

BLAST of PI0007055 vs. NCBI nr
Match: XP_008461387.1 (PREDICTED: uncharacterized protein LOC103499982 [Cucumis melo])

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 594/652 (91.10%), Postives = 605/652 (92.79%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           MDNPTGAVLFTTIGLNQYGFDIFSV LNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE
Sbjct: 1   MDNPTGAVLFTTIGLNQYGFDIFSVALNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGSSRIYLSDSPNSSPKLLASAP SCFHDRPI                 PFTSWAALYS
Sbjct: 61  RSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVRNGRLLFISAHENPHKPFTSWAALYS 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           TPLDG+DS+TRLTPLGSVDFSPAVS SGKFVAVASYGSRSWGGEFHELN EIVVFKSSDP
Sbjct: 121 TPLDGDDSITRLTPLGSVDFSPAVSGSGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYV---SPVPIRVTPA 240
           DRRVVVA RGGWPSWSGDSTVFFHRKA+DGWWSIFKVEIPENLDS +   SPVPIRVTPA
Sbjct: 181 DRRVVVASRGGWPSWSGDSTVFFHRKAEDGWWSIFKVEIPENLDSSMSSDSPVPIRVTPA 240

Query: 241 GLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYN 300
           GLHCFTPA+MNDSR VVVATRRAD     SKFRHIEIFDS LEEFIPITQKLNPDFHHYN
Sbjct: 241 GLHCFTPAAMNDSRLVVVATRRAD-----SKFRHIEIFDSELEEFIPITQKLNPDFHHYN 300

Query: 301 PFVSPDSNFIGYHRFRGESAQGELIPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFN 360
           PFVSPDSNFIGYHRFRGES Q ELIPYL PVISPIKELQMIRINGSFP PSPDGDLIAFN
Sbjct: 301 PFVSPDSNFIGYHRFRGESTQSELIPYLSPVISPIKELQMIRINGSFPAPSPDGDLIAFN 360

Query: 361 PDFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITI 420
           P+FNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAV ATVQIARITI
Sbjct: 361 PNFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVTATVQIARITI 420

Query: 421 NSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANG 480
           NSDDL  GDSDEV+SEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDA  G
Sbjct: 421 NSDDLKDGDSDEVSSEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAVKG 480

Query: 481 EFDGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540
           EF+GELRRLTDG WIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV
Sbjct: 481 EFEGELRRLTDGAWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540

Query: 541 AGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT 600
           AGPEGSS+VDRERINHVCFSRDGKWLLFTANLSGVTAEPVS PNQFQPYGDLFVVRLDGT
Sbjct: 541 AGPEGSSEVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSWPNQFQPYGDLFVVRLDGT 600

Query: 601 GLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           GL RLTWN YENGTPTWYYGSE+ALSGLSLKDEVVG+KLKG FDEPLWITFD
Sbjct: 601 GLSRLTWNGYENGTPTWYYGSEVALSGLSLKDEVVGEKLKGNFDEPLWITFD 647

BLAST of PI0007055 vs. NCBI nr
Match: XP_004135965.1 (uncharacterized protein LOC101214858 [Cucumis sativus] >KGN45063.1 hypothetical protein Csa_015982 [Cucumis sativus])

HSP 1 Score: 1187.2 bits (3070), Expect = 0.0e+00
Identity = 584/652 (89.57%), Postives = 601/652 (92.18%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           MDNPTGAVLFTT+GLNQYGFDIFSVPLNS TVER+LTDGISVNFNAQFLNNQLSVVFISE
Sbjct: 1   MDNPTGAVLFTTVGLNQYGFDIFSVPLNSLTVERQLTDGISVNFNAQFLNNQLSVVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGSSRIYLSDSPNSSPKLLASAP SCFHDRPI                 PFTSWAALYS
Sbjct: 61  RSGSSRIYLSDSPNSSPKLLASAPGSCFHDRPIVTNGRLLFISAHENPHKPFTSWAALYS 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           TPLDG DSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELN EIVVFKSSDP
Sbjct: 121 TPLDGHDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNLEIVVFKSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLD---SYVSPVPIRVTPA 240
            +RVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLD   S VSPV IRVTPA
Sbjct: 181 GQRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSSRSSVSPVAIRVTPA 240

Query: 241 GLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYN 300
           GLHCFTPA+MND RRVVVATRRAD     SK+RHIEIFDS LEEFIPITQKLNP+FHHYN
Sbjct: 241 GLHCFTPAAMNDGRRVVVATRRAD-----SKYRHIEIFDSELEEFIPITQKLNPEFHHYN 300

Query: 301 PFVSPDSNFIGYHRFRGESAQGELIPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFN 360
           PFVSPDSNFIGYHRFRGES Q ELIPYL PVISPIKELQ+IR+NGSFPTPSPDGDLIAFN
Sbjct: 301 PFVSPDSNFIGYHRFRGESTQSELIPYLYPVISPIKELQIIRVNGSFPTPSPDGDLIAFN 360

Query: 361 PDFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITI 420
           P F GLQIVKFDGSKCRTVLKDRTAF NSWSPTEKNVIYTSLGPIFGAV ATVQIARITI
Sbjct: 361 PGFIGLQIVKFDGSKCRTVLKDRTAFCNSWSPTEKNVIYTSLGPIFGAVTATVQIARITI 420

Query: 421 NSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANG 480
           N     SGDSDEV++EVKILTKD+TGNNAFPACSPDGKFLVFRSGR+GHKNLYIVDA  G
Sbjct: 421 N-----SGDSDEVSNEVKILTKDNTGNNAFPACSPDGKFLVFRSGRTGHKNLYIVDAMKG 480

Query: 481 EFDGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540
           EF+GELR+LTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV
Sbjct: 481 EFEGELRQLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYV 540

Query: 541 AGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT 600
           AGPEGSS+VDRERINHVCFSRDG WLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT
Sbjct: 541 AGPEGSSEVDRERINHVCFSRDGNWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGT 600

Query: 601 GLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           GLRRLT NAYENGTPTWYYGSELALSGLSLKDEVVG+KLKG+FDEPLWI FD
Sbjct: 601 GLRRLTCNAYENGTPTWYYGSELALSGLSLKDEVVGEKLKGDFDEPLWIKFD 642

BLAST of PI0007055 vs. NCBI nr
Match: XP_038899648.1 (uncharacterized protein LOC120086912 [Benincasa hispida])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 572/653 (87.60%), Postives = 595/653 (91.12%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           MDNPTGAVLFTTIGL QYGFDIFSVPLNSPT E RLTDGISVNFNAQFL+NQLSVVFISE
Sbjct: 1   MDNPTGAVLFTTIGLQQYGFDIFSVPLNSPTTEHRLTDGISVNFNAQFLDNQLSVVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGSSRIYLSDSPNS+PKLL SAP S FHDRPI                 PFTSW+ALYS
Sbjct: 61  RSGSSRIYLSDSPNSAPKLLPSAPGSFFHDRPIVRNDRLFFISAHENPQKPFTSWSALYS 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           T LDG DS+TRLTP GSVDFSPAVS+SGKFVAVASY SRSWGGEFHEL+T IVVFKSSDP
Sbjct: 121 TSLDGGDSITRLTPPGSVDFSPAVSKSGKFVAVASYESRSWGGEFHELHTAIVVFKSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLD---SYVSPVPIRVTPA 240
           +RRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPE LD   S VSPVPIRVTPA
Sbjct: 181 NRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPEKLDSSVSSVSPVPIRVTPA 240

Query: 241 GLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYN 300
           GLHCFTPA++NDS+R+ VATRRAD     SKFRHIEIF+S  +EFIPITQKLNPDFHHYN
Sbjct: 241 GLHCFTPAALNDSKRLAVATRRAD-----SKFRHIEIFNSESDEFIPITQKLNPDFHHYN 300

Query: 301 PFVSPDSNFIGYHRFRGESAQGEL-IPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAF 360
           PF+SPDSN IGYHRFRGESAQ EL IPYL PVISPIKEL+MIRINGSFPTPSPDGDLIAF
Sbjct: 301 PFISPDSNSIGYHRFRGESAQSELTIPYLDPVISPIKELRMIRINGSFPTPSPDGDLIAF 360

Query: 361 NPDFNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARIT 420
           NP+FNGLQI+K DGSKC TVLKDRTAFYNSWSP+EKNVIYTSLGPIFG V  TVQIARIT
Sbjct: 361 NPNFNGLQIIKSDGSKCWTVLKDRTAFYNSWSPSEKNVIYTSLGPIFGEVTVTVQIARIT 420

Query: 421 INSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAAN 480
           INSDDL SGD DEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYI+DA N
Sbjct: 421 INSDDLNSGDGDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVN 480

Query: 481 GEFDGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVY 540
           GEFDGELRRLTDGPWIDTMP+WSP GDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRV+
Sbjct: 481 GEFDGELRRLTDGPWIDTMPSWSPTGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVH 540

Query: 541 VAGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDG 600
           VAGPEGSSDV RERINHVCFSRDG+WLLFTANLSGVTAEPVS PNQFQPYGDLFVVRLDG
Sbjct: 541 VAGPEGSSDVGRERINHVCFSRDGEWLLFTANLSGVTAEPVSFPNQFQPYGDLFVVRLDG 600

Query: 601 TGLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           TGLRRLTWN YENGTPTWYYGSELAL GLSLKDEVVG+KLKGEFDEPLWITFD
Sbjct: 601 TGLRRLTWNGYENGTPTWYYGSELALYGLSLKDEVVGEKLKGEFDEPLWITFD 648

BLAST of PI0007055 vs. NCBI nr
Match: XP_023547812.1 (uncharacterized protein LOC111806666 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 537/650 (82.62%), Postives = 582/650 (89.54%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQLSVVFISE 60
           M NPTG V+FTT+G  QYGFD FSVPLNSPT E  LTDGISVNFNAQF++NQLS+VFISE
Sbjct: 1   MTNPTGTVIFTTVGRTQYGFDTFSVPLNSPTTEHCLTDGISVNFNAQFVDNQLSIVFISE 60

Query: 61  RSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI-----------------PFTSWAALYS 120
           RSGS R+YLS+SPNS+PKLL SAP SCFHDRPI                 PFTSW+ALY 
Sbjct: 61  RSGSPRVYLSNSPNSAPKLLPSAPGSCFHDRPIIRNDRLYFISAHENPHKPFTSWSALYF 120

Query: 121 TPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDP 180
           T LDG DSVTRLTP GSVDFSPAVSESGKFVAVASYGSRSWGGEF EL+TEIVVF+SSDP
Sbjct: 121 TGLDGSDSVTRLTPRGSVDFSPAVSESGKFVAVASYGSRSWGGEFQELHTEIVVFRSSDP 180

Query: 181 DRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYVSPVPIRVTPAGLH 240
           DRRVVV+GRGGWPSWSGDSTV+FHR+A+DGWWSIF+VEIPENLDS V PVPIRVTPAGLH
Sbjct: 181 DRRVVVSGRGGWPSWSGDSTVYFHRQAEDGWWSIFRVEIPENLDSSVPPVPIRVTPAGLH 240

Query: 241 CFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYNPFV 300
           CFTPA+MND +RVVVATRR D     +KFRHIEI++S  +EF PITQKLNP FHHYNPFV
Sbjct: 241 CFTPAAMNDRKRVVVATRRPD-----NKFRHIEIYNSGTDEFDPITQKLNPSFHHYNPFV 300

Query: 301 SPDSNFIGYHRFRGESAQGEL-IPYLKPVISPIKELQMIRINGSFPTPSPDGDLIAFNPD 360
           SPDSN+IGYHRFRGES+ GEL IP+ + +ISPI EL+MIRINGSFPT SPDG+ IAFNPD
Sbjct: 301 SPDSNYIGYHRFRGESSHGELTIPHFERIISPINELRMIRINGSFPTQSPDGNFIAFNPD 360

Query: 361 FNGLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITINS 420
           F GL+IVK DGSKC TVLKDRTAFYNSWSPTEKNVIY+SLGPIFG  RATVQIAR TINS
Sbjct: 361 FVGLRIVKADGSKCVTVLKDRTAFYNSWSPTEKNVIYSSLGPIFGPARATVQIARTTINS 420

Query: 421 DDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANGEF 480
           DDL +GDSDEVA EVKILTK+DTGNNAFPACSPDGKFLVFRSGRSGHKNLYI+DA NG+F
Sbjct: 421 DDLNNGDSDEVAGEVKILTKEDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIIDAVNGDF 480

Query: 481 DGELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAG 540
            GE RRLTDGPWIDTMP+WSP GDLI FSSNMHNPKNTEAFSIYVIRPDGS LRRV+VAG
Sbjct: 481 GGEARRLTDGPWIDTMPSWSPAGDLIAFSSNMHNPKNTEAFSIYVIRPDGSNLRRVHVAG 540

Query: 541 PEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLFVVRLDGTGL 600
           PEGSSDVD+ERINHVCFSRDG+WLLFT+NL GV+AEPVS+PNQFQPYGDLFVVRLDGTGL
Sbjct: 541 PEGSSDVDKERINHVCFSRDGEWLLFTSNLGGVSAEPVSMPNQFQPYGDLFVVRLDGTGL 600

Query: 601 RRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           RRLTW+AYENGTPTWYYGSELALSGLSLKDEVVG+KLKGEFDEPLWITF+
Sbjct: 601 RRLTWSAYENGTPTWYYGSELALSGLSLKDEVVGEKLKGEFDEPLWITFN 645

BLAST of PI0007055 vs. TAIR 10
Match: AT4G01870.1 (tolB protein-related )

HSP 1 Score: 848.6 bits (2191), Expect = 3.3e-246
Identity = 407/659 (61.76%), Postives = 511/659 (77.54%), Query Frame = 0

Query: 1   MDNPTGAVLFTTIGLNQYGFDIFSVPLNSPTVERRLTDGISVNFNAQFLNNQL-SVVFIS 60
           M+ P G ++FTT+G   YGFD+FS+ + + +VERRLTDG+SVNFNAQF+N++   VVF+S
Sbjct: 1   METPKGTIIFTTVGRTHYGFDVFSLNI-ATSVERRLTDGVSVNFNAQFVNDKSDDVVFVS 60

Query: 61  ERSGSSRIYLSDSPNSSPKLLASAPRSCFHDRPI------------------PFTSWAAL 120
           ER+GS+RIY + S  S P+ +  AP S FHDRPI                   F +W+AL
Sbjct: 61  ERNGSARIYKTRSGISKPEQIPGAPESYFHDRPIITQNNRLYFISAHEQPDRYFKNWSAL 120

Query: 121 YSTPLD-GEDSVTRLTPLGSVDFSPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKS 180
           Y+  L+  +  VTR+TP  + DFSPAVS+SG F+AVASYG+RSWGGEFHE+NT+I VFK+
Sbjct: 121 YTVELNSAKREVTRVTPPDTADFSPAVSQSGDFLAVASYGTRSWGGEFHEINTDITVFKA 180

Query: 181 SDPDRRVVVAGRGGWPSWSGDSTVFFHRKADDGWWSIFKVEIPENLDSYVS-PV-PIRVT 240
           S P+ RVV+  RGGWP+WSGDSTVFFH +ADDGWWSIF+V+IPEN   Y   P+ PIRVT
Sbjct: 181 SKPETRVVICERGGWPTWSGDSTVFFHHQADDGWWSIFRVDIPENFTEYTDFPITPIRVT 240

Query: 241 PAGLHCFTPASMNDSRRVVVATRRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHH 300
           P+GLHCFTPA+  D +R+ +ATRR     +    RHIEI+D     F P+T+ LNP FHH
Sbjct: 241 PSGLHCFTPAAFRDGKRIALATRR-----RGVNHRHIEIYDLENTTFQPVTESLNPSFHH 300

Query: 301 YNPFVSPDSNFIGYHRFRGESAQGE-LIPYLKPVISPIKELQMIRINGSFPTPSPDGDLI 360
           YNPFVSPDS F+GYHRFRGES QGE ++P ++ ++SPIK L+++RINGSFP+ SP+GDLI
Sbjct: 301 YNPFVSPDSEFLGYHRFRGESTQGESIVPNIESIVSPIKTLRLLRINGSFPSSSPNGDLI 360

Query: 361 AFNPDFN---GLQIVKFDGSKCRTVLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQ 420
           A N DF+   G+++ K DGSK  T++KDRTAFYNSWSPTE++VIYTSLGPIF   R  VQ
Sbjct: 361 ALNSDFDINGGIKVSKSDGSKRWTLIKDRTAFYNSWSPTERHVIYTSLGPIFSPARIAVQ 420

Query: 421 IARITINSDDLISGDSDEVASEVKILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYI 480
           IARI  +  DL + D +++  +VKILT ++TGNNAFP+CSPDGK +VFRSGRSGHKNLYI
Sbjct: 421 IARIKFDPSDL-TADKEDLPCDVKILTLENTGNNAFPSCSPDGKSIVFRSGRSGHKNLYI 480

Query: 481 VDAANGEFD-GELRRLTDGPWIDTMPNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGS 540
           VDA NGE + G +RRLTDGPWIDTMP WSP+GDLI FSSN HNP+NT  F  YV+RPDG+
Sbjct: 481 VDAVNGESNGGGIRRLTDGPWIDTMPCWSPKGDLIGFSSNRHNPENTAVFGAYVVRPDGT 540

Query: 541 GLRRVYVAGPEGSSDVDRERINHVCFSRDGKWLLFTANLSGVTAEPVSLPNQFQPYGDLF 600
           GLRR+ ++GPEGS +  RER+NHV F++DG WL+F ANLSGVTAEPV++PNQFQPYGDL+
Sbjct: 541 GLRRIQISGPEGSEEAARERVNHVSFNKDGDWLVFAANLSGVTAEPVTMPNQFQPYGDLY 600

Query: 601 VVRLDGTGLRRLTWNAYENGTPTWYYGSELALSGLSLKDEVVGQKLKGEFDEPLWITFD 633
           VV+LDGTGLRRLTWN YE+GTPTW+   EL LS L+L  +  G KL+G+F+EPLWI+ D
Sbjct: 601 VVKLDGTGLRRLTWNGYEDGTPTWHTADELDLSQLNLNGQ-DGDKLEGQFEEPLWISCD 651

BLAST of PI0007055 vs. TAIR 10
Match: AT1G21680.1 (DPP6 N-terminal domain-like protein )

HSP 1 Score: 465.3 bits (1196), Expect = 7.8e-131
Identity = 273/659 (41.43%), Postives = 376/659 (57.06%), Query Frame = 0

Query: 8   VLFTTIGLNQYGFDIFSVPLNSP---TVERRLTDGISVNFNAQFL--------------- 67
           ++FTT+G + Y FDIF++    P   + E R+TDG SVNFN  F                
Sbjct: 34  IIFTTLGRSHYEFDIFALSTTQPPSVSGELRITDGESVNFNGYFPSPSPALLSLLPDETL 93

Query: 68  -----NNQLSVVFISERSGSSRIYL-------SDSPNSSPKLLASAPR------------ 127
                ++ L +++++ER+G+S +Y        SD       +L +  R            
Sbjct: 94  IQMEDSSPLHLIYVTERNGTSSLYYDLVYGGNSDFKTKRRSVLEAPSRVQVPLLSRFDHL 153

Query: 128 -----SCFHDRPI-----------------PFTSWAALYSTPLDGEDSVTRLTPLGSVDF 187
                + F D+P                  P  SW A+YST L       RLTP G  DF
Sbjct: 154 SGMTVNSFKDKPSLSGEFIVYVSTHESSGEPRASWTAVYSTELK-TGLTRRLTPSGVADF 213

Query: 188 SPAVSESGKFVAVASYGSRSWGGEFHELNTEIVVFKSSDPDRRVVVAGRGGWPSWSGDST 247
           SPAVS SG   AVASYG R W GE  EL T+I VF + D   RV V   GGWP W  +ST
Sbjct: 214 SPAVSPSGNLTAVASYGERGWTGEVEELRTDIYVFLTRDGSHRVKVVEHGGWPCWVDEST 273

Query: 248 VFFHRKA-DDGWWSIFKVEIPENLDSYVSPVPI-RVTPAGLHCFTPA-SMNDSRRVVVAT 307
           ++FHR++ +DGW S+++  +PEN       V I RVTP G+H FTPA S N+   V VAT
Sbjct: 274 LYFHRRSEEDGWISVYRAILPENGPLTTESVTIQRVTPPGVHAFTPATSPNNHEFVAVAT 333

Query: 308 RRADSKFQHSKFRHIEIFDSVLEEFIPITQKLNPDFHHYNPFVSPDSNFIGYHRFRGESA 367
           RR       S +RH+E+FD    EFI +T+ + P  HH NPF+SPDS+ +GYH  RG+ A
Sbjct: 334 RRPG-----SDYRHVELFDLKRNEFIELTRLVAPKSHHLNPFLSPDSSRVGYHSCRGD-A 393

Query: 368 QGELIP--YLKPVISPIKELQMIRINGSFPTPSPDGDLIAFNPDFNGLQIVKFDGSKCRT 427
            G   P  +L+ + +  ++L + RI+GSFP+ SP GD IA+     G+ +VK DGS  R 
Sbjct: 394 NGRRSPLLFLENIQTTTRDLSLFRIDGSFPSFSPGGDRIAY-VKMPGVFVVKPDGSGQRE 453

Query: 428 VLKDRTAFYNSWSPTEKNVIYTSLGPIFGAVRATVQIARITINSDDLISGDSDEVASEVK 487
           V K   AF  +W P    ++Y+S GP F   R  V +  I +        D+ + +S V+
Sbjct: 454 VYKG-MAFSTAWDPVRPGIVYSSSGPTFATERTEVDVISIDV--------DAADKSSSVR 513

Query: 488 ILTKDDTGNNAFPACSPDGKFLVFRSGRSGHKNLYIVDAANGEFDGELRRLTDGPWIDTM 547
            LT +   NNAFP  SPDGK +VFRSGR+GHKNLYI+DA  GE  G L RLT+G W DTM
Sbjct: 514 RLTTNGK-NNAFPWPSPDGKRIVFRSGRTGHKNLYIMDAEKGE-SGGLWRLTEGAWTDTM 573

Query: 548 PNWSPRGDLIVFSSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGPEGSSDVDRERINHVC 598
            NWSP G+ I F+S+  +P  + +F +++I P+G+GLR++  +G  G       R NH  
Sbjct: 574 CNWSPDGEWIAFASDRESP-GSGSFELFLIHPNGTGLRKLIQSGTGG-------RTNHPI 633

BLAST of PI0007055 vs. TAIR 10
Match: AT1G21670.1 (LOCATED IN: cell wall, plant-type cell wall; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: WD40-like Beta Propeller (InterPro:IPR011659), Six-bladed beta-propeller, TolB-like (InterPro:IPR011042); BEST Arabidopsis thaliana protein match is: DPP6 N-terminal domain-like protein (TAIR:AT1G21680.1); Has 8461 Blast hits to 5060 proteins in 1257 species: Archae - 79; Bacteria - 5567; Metazoa - 37; Fungi - 70; Plants - 117; Viruses - 0; Other Eukaryotes - 2591 (source: NCBI BLink). )

HSP 1 Score: 421.4 bits (1082), Expect = 1.3e-117
Identity = 259/648 (39.97%), Postives = 363/648 (56.02%), Query Frame = 0

Query: 8   VLFTTIGLNQYGFDIFSVPLN----SPTVERRLTDGISVNFNAQFLN------------- 67
           +LFTTIG   + FDIF++P +    SP  E RLTDG S+NFN  F +             
Sbjct: 34  ILFTTIGRPTFEFDIFTLPTSHRPPSPADEHRLTDGKSINFNGYFASPSTALISLLPKRT 93

Query: 68  ----NQLSVVFISERSGS--------------SRIYLS-------------DSPNSSPKL 127
                 + +++++ER+G+              SRI +              +S   +P L
Sbjct: 94  QIQPQDVHLIYVTERAGTPSLNYDVVHSDNVGSRIQVPLFSGEEQQSGMNVNSMKDTPVL 153

Query: 128 L-ASAPRSCFHDRP-IPFTSWAALYSTPLDGEDSVTRLTPLGSVDFSPAVSESGKFVAVA 187
                     H+ P  P  SWAA+YST L  + S  RLTPLG  DFSPAVS SGK+ AVA
Sbjct: 154 TNGYLVHVSTHENPGKPMASWAAVYSTELRTK-STRRLTPLGIADFSPAVSPSGKWTAVA 213

Query: 188 SYGSRSWGGEF--HELNTEIVVFKSSDPDRRVVVAGRGGWPSWSGDSTVFFHRKADDGWW 247
           S+G + W       E+++++ VF + D  +RV V  +GGWP W  DST++FHRK+DDGW 
Sbjct: 214 SFGEKGWTWSMVEKEISSDVYVFLTQDGTQRVKVVEQGGWPRWVDDSTLYFHRKSDDGWI 273

Query: 248 SIFKVEIPENLDSYVSPVPI-RVTPAGLHCFTPA-SMNDSRRVVVATRRADSKFQHSKFR 307
           S+++  +P+        V I RVTP GLH FTPA S N++  + VATRR       S+ R
Sbjct: 274 SVYRAILPKTGPVTTKSVTIQRVTPPGLHAFTPATSPNNNNFIAVATRRPG-----SEIR 333

Query: 308 HIEIFDSVLEEFIPITQKLNPDFHHYNPFVSPDSNFIGYHRFRGESAQGELIP--YLKPV 367
           H+E+FD    EF+ +T+ ++P  HH+NPF+SPDS+ +GYH  RG+ A G   P   L+ +
Sbjct: 334 HVELFDLKKNEFVELTRLVSPKSHHFNPFLSPDSSRVGYHSCRGD-ATGRKTPRNLLQSL 393

Query: 368 ISPIKELQMIRINGSFPTPSPDGDLIAFNPDFNGLQIVKFDGSKCRTVLKDRTAFYNSWS 427
            +   +L + R +G+FP+ SP+GD  AF   F G+ +V  DGS  R +L  +  F   W 
Sbjct: 394 KTTSNDLSLFRFDGAFPSISPEGDRFAF-VSFTGVFVVNPDGSGLRQLL-PQMGFGTVWD 453

Query: 428 PTEKNVIYTSLGPIFGAVRATVQIARITINSDDLISGDSDEVASEVKILTKDDTG-NNAF 487
           P    ++YTS GP     ++ + I  I +        D+   A+ VK LT   TG NNAF
Sbjct: 454 PIRHGIVYTSSGPALAPGKSQIDILAINV--------DAPSPATAVKKLT--TTGENNAF 513

Query: 488 PACSPDGKFLVFRSGRSGHKNLYIVDAANGEFDGELRRLTDGPWIDTMPNWSPRGDLIVF 547
           P  SPDGK +VFRS RSG KNLYI+DA  GE  G L RLT+G W DT+  WSP G+ IVF
Sbjct: 514 PWPSPDGKRIVFRSARSGTKNLYIMDAEKGE-SGGLFRLTNGNWNDTIATWSPDGNWIVF 573

Query: 548 SSNMHNPKNTEAFSIYVIRPDGSGLRRVYVAGPEGSSDVDRERINHVCFSRDGKWLLFTA 599
           +SN   P  T   +IYV+ PDG+GLR++       + ++      H  FS D K ++FT 
Sbjct: 574 ASNREFP-GTLLMNIYVVHPDGTGLRKL-------AQNLTGLVSMHPMFSPDSKRIVFTT 633

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q39PS96.2e-1628.52Tol-Pal system protein TolB OS=Geobacter metallireducens (strain ATCC 53774 / DS... [more]
Q74H675.2e-1528.21Tol-Pal system protein TolB OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM... [more]
A8LHQ67.6e-1435.48Tol-Pal system protein TolB OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB ... [more]
Q3APB52.2e-1329.89Protein TolB homolog OS=Chlorobium chlorochromatii (strain CaD3) OX=340177 GN=to... [more]
Q167Z66.4e-1337.41Tol-Pal system protein TolB OS=Roseobacter denitrificans (strain ATCC 33942 / OC... [more]
Match NameE-valueIdentityDescription
A0A5A7URS20.0e+0091.56TolB protein-related isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CEJ80.0e+0091.10uncharacterized protein LOC103499982 OS=Cucumis melo OX=3656 GN=LOC103499982 PE=... [more]
A0A0A0K6G20.0e+0089.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G419590 PE=4 SV=1[more]
A0A6J1GRK10.0e+0081.69uncharacterized protein LOC111456480 OS=Cucurbita moschata OX=3662 GN=LOC1114564... [more]
A0A6J1JTR30.0e+0080.77uncharacterized protein LOC111488250 OS=Cucurbita maxima OX=3661 GN=LOC111488250... [more]
Match NameE-valueIdentityDescription
KAA0058562.10.0e+0091.56TolB protein-related isoform 1 [Cucumis melo var. makuwa] >TYK10360.1 TolB prote... [more]
XP_008461387.10.0e+0091.10PREDICTED: uncharacterized protein LOC103499982 [Cucumis melo][more]
XP_004135965.10.0e+0089.57uncharacterized protein LOC101214858 [Cucumis sativus] >KGN45063.1 hypothetical ... [more]
XP_038899648.10.0e+0087.60uncharacterized protein LOC120086912 [Benincasa hispida][more]
XP_023547812.10.0e+0082.62uncharacterized protein LOC111806666 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G01870.13.3e-24661.76tolB protein-related [more]
AT1G21680.17.8e-13141.43DPP6 N-terminal domain-like protein [more]
AT1G21670.11.3e-11739.97LOCATED IN: cell wall, plant-type cell wall; EXPRESSED IN: 23 plant structures; ... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 90..311
e-value: 2.2E-10
score: 42.3
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 312..529
e-value: 4.1E-33
score: 116.8
IPR011042Six-bladed beta-propeller, TolB-likeGENE3D2.120.10.30coord: 530..607
e-value: 1.7E-6
score: 29.5
IPR011659WD40-like Beta PropellerPFAMPF07676PD40coord: 466..493
e-value: 0.058
score: 13.4
coord: 425..454
e-value: 3.4E-4
score: 20.5
NoneNo IPR availablePANTHERPTHR32161DPP6 N-TERMINAL DOMAIN-LIKE PROTEINcoord: 3..632
NoneNo IPR availablePANTHERPTHR32161:SF9TOLB PROTEIN-LIKE PROTEINcoord: 3..632
NoneNo IPR availableSUPERFAMILY82171DPP6 N-terminal domain-likecoord: 176..593
NoneNo IPR availableSUPERFAMILY69304Tricorn protease N-terminal domaincoord: 6..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0007055.1PI0007055.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis