PI0006945 (gene) Melon (PI 482460) v1

Overview
NamePI0006945
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionDNA-directed RNA polymerase
Locationchr04: 16153157 .. 16153618 (+)
RNA-Seq ExpressionPI0006945
SyntenyPI0006945
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACATCAAAAGCTTTCGGAATTGGGGATCTCTGGTACCAAAGAATGTGGAGAAACATTTGCAAAAGGGCTGCTTCAAGGAAAGCTACATTATTCTCCAAGTTGTCTTCTTCTAGCTCAAGAAAAGTTACTGAAGACTATATCTTTCTTGATCAAATTAGATCTTCTAGGTCGCTAAAAAGCTTGAACGCTTGTTGCCAATCAGGGGGTTCTAGTAGAATCGGCTTTCTCCATCCCCGCAAAGTTGGATTTACGAACTCCAATTTCCCACATTCAAGCAATCCCGTGGCGTTTTATGGGGTTCTAGACCATGCTAAAGGCTATGCTACTGCTGCCAAGGCAGCCATTGTCGAAGGGGATTTATTGGGTTTCGAAGAAATTCAGGAAATGATGGAGGAGCTCAGCAAACAAGATAAGGTGGAGCCTCACTTTAAGAAGCCTAGAGGATGGTCAATGGAATAG

mRNA sequence

ATGACATCAAAAGCTTTCGGAATTGGGGATCTCTGGTACCAAAGAATGTGGAGAAACATTTGCAAAAGGGCTGCTTCAAGGAAAGCTACATTATTCTCCAAGTTGTCTTCTTCTAGCTCAAGAAAAGTTACTGAAGACTATATCTTTCTTGATCAAATTAGATCTTCTAGGTCGCTAAAAAGCTTGAACGCTTGTTGCCAATCAGGGGGTTCTAGTAGAATCGGCTTTCTCCATCCCCGCAAAGTTGGATTTACGAACTCCAATTTCCCACATTCAAGCAATCCCGTGGCGTTTTATGGGGTTCTAGACCATGCTAAAGGCTATGCTACTGCTGCCAAGGCAGCCATTGTCGAAGGGGATTTATTGGGTTTCGAAGAAATTCAGGAAATGATGGAGGAGCTCAGCAAACAAGATAAGGTGGAGCCTCACTTTAAGAAGCCTAGAGGATGGTCAATGGAATAG

Coding sequence (CDS)

ATGACATCAAAAGCTTTCGGAATTGGGGATCTCTGGTACCAAAGAATGTGGAGAAACATTTGCAAAAGGGCTGCTTCAAGGAAAGCTACATTATTCTCCAAGTTGTCTTCTTCTAGCTCAAGAAAAGTTACTGAAGACTATATCTTTCTTGATCAAATTAGATCTTCTAGGTCGCTAAAAAGCTTGAACGCTTGTTGCCAATCAGGGGGTTCTAGTAGAATCGGCTTTCTCCATCCCCGCAAAGTTGGATTTACGAACTCCAATTTCCCACATTCAAGCAATCCCGTGGCGTTTTATGGGGTTCTAGACCATGCTAAAGGCTATGCTACTGCTGCCAAGGCAGCCATTGTCGAAGGGGATTTATTGGGTTTCGAAGAAATTCAGGAAATGATGGAGGAGCTCAGCAAACAAGATAAGGTGGAGCCTCACTTTAAGAAGCCTAGAGGATGGTCAATGGAATAG

Protein sequence

MTSKAFGIGDLWYQRMWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIGFLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELSKQDKVEPHFKKPRGWSME
Homology
BLAST of PI0006945 vs. ExPASy TrEMBL
Match: A0A5A7SYG2 (DNA-directed RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00320 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.4e-54
Identity = 111/133 (83.46%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRN+ KRAASRKATLFS+LSSS+SRKVTED+IFLDQIR SRSL++LN CCQSG SSRI 
Sbjct: 1   MWRNVLKRAASRKATLFSELSSSTSRKVTEDHIFLDQIRYSRSLETLNTCCQSGDSSRIS 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP+KVGFTNSNFPHSSNPVAFYGVL+HA+GYATAA+AAI EGDL G EEIQE+ME +S
Sbjct: 61  FLHPQKVGFTNSNFPHSSNPVAFYGVLNHAEGYATAAEAAISEGDLSGSEEIQEIMEGIS 120

Query: 136 KQDKVEPHFKKPR 149
           KQDKVEPHFKKP+
Sbjct: 121 KQDKVEPHFKKPK 133

BLAST of PI0006945 vs. ExPASy TrEMBL
Match: A0A1S3BF59 (DNA-directed RNA polymerase OS=Cucumis melo OX=3656 GN=LOC103489205 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.4e-54
Identity = 111/133 (83.46%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRN+ KRAASRKATLFS+LSSS+SRKVTED+IFLDQIR SRSL++LN CCQSG SSRI 
Sbjct: 1   MWRNVLKRAASRKATLFSELSSSTSRKVTEDHIFLDQIRYSRSLETLNTCCQSGDSSRIS 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP+KVGFTNSNFPHSSNPVAFYGVL+HA+GYATAA+AAI EGDL G EEIQE+ME +S
Sbjct: 61  FLHPQKVGFTNSNFPHSSNPVAFYGVLNHAEGYATAAEAAISEGDLSGSEEIQEIMEGIS 120

Query: 136 KQDKVEPHFKKPR 149
           KQDKVEPHFKKP+
Sbjct: 121 KQDKVEPHFKKPK 133

BLAST of PI0006945 vs. ExPASy TrEMBL
Match: A0A1S3BF55 (DNA-directed RNA polymerase OS=Cucumis melo OX=3656 GN=LOC103489205 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.4e-54
Identity = 111/133 (83.46%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRN+ KRAASRKATLFS+LSSS+SRKVTED+IFLDQIR SRSL++LN CCQSG SSRI 
Sbjct: 1   MWRNVLKRAASRKATLFSELSSSTSRKVTEDHIFLDQIRYSRSLETLNTCCQSGDSSRIS 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP+KVGFTNSNFPHSSNPVAFYGVL+HA+GYATAA+AAI EGDL G EEIQE+ME +S
Sbjct: 61  FLHPQKVGFTNSNFPHSSNPVAFYGVLNHAEGYATAAEAAISEGDLSGSEEIQEIMEGIS 120

Query: 136 KQDKVEPHFKKPR 149
           KQDKVEPHFKKP+
Sbjct: 121 KQDKVEPHFKKPK 133

BLAST of PI0006945 vs. ExPASy TrEMBL
Match: A0A0A0KR04 (DNA-directed RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_5G607420 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 3.0e-52
Identity = 109/134 (81.34%), Postives = 121/134 (90.30%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRN+ K AASRKA LFS+ SSS+SRKVTED+IFLDQIRSSRSL++LN CCQSGGSSRI 
Sbjct: 1   MWRNVFKTAASRKAKLFSEFSSSTSRKVTEDHIFLDQIRSSRSLETLNTCCQSGGSSRII 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP+KVGFTNSNFPHSSNPV FYGVL HAKGYATAA+AAI EGDL G EEIQE+ME L+
Sbjct: 61  FLHPQKVGFTNSNFPHSSNPVPFYGVLYHAKGYATAAEAAIFEGDLSGSEEIQEIMEGLN 120

Query: 136 KQDKVEPHFKKPRG 150
           KQDKVE HFK+P+G
Sbjct: 121 KQDKVELHFKQPKG 134

BLAST of PI0006945 vs. ExPASy TrEMBL
Match: A0A6J1HS83 (DNA-directed RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111467347 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 4.7e-37
Identity = 88/133 (66.17%), Postives = 101/133 (75.94%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRNI KRAASR+ T F       SRKV ED IF+++IRSSR+L SL +CC   GS  I 
Sbjct: 1   MWRNIVKRAASRRDTFF-------SRKVIEDSIFVEEIRSSRTLGSLKSCCLLEGSRGIN 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           F H R+VGF  SN  HSS P+AFYGVL+HAKGYATAA+AAI E DL G EEIQE+MEELS
Sbjct: 61  FFHSREVGFAKSNCAHSSEPLAFYGVLNHAKGYATAAEAAISEEDLSGSEEIQELMEELS 120

Query: 136 KQDKVEPHFKKPR 149
           KQDKVE HFK+P+
Sbjct: 121 KQDKVESHFKQPK 126

BLAST of PI0006945 vs. NCBI nr
Match: XP_008446483.1 (PREDICTED: DNA-directed RNA polymerase 1B, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 222.2 bits (565), Expect = 3.0e-54
Identity = 111/133 (83.46%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRN+ KRAASRKATLFS+LSSS+SRKVTED+IFLDQIR SRSL++LN CCQSG SSRI 
Sbjct: 1   MWRNVLKRAASRKATLFSELSSSTSRKVTEDHIFLDQIRYSRSLETLNTCCQSGDSSRIS 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP+KVGFTNSNFPHSSNPVAFYGVL+HA+GYATAA+AAI EGDL G EEIQE+ME +S
Sbjct: 61  FLHPQKVGFTNSNFPHSSNPVAFYGVLNHAEGYATAAEAAISEGDLSGSEEIQEIMEGIS 120

Query: 136 KQDKVEPHFKKPR 149
           KQDKVEPHFKKP+
Sbjct: 121 KQDKVEPHFKKPK 133

BLAST of PI0006945 vs. NCBI nr
Match: XP_008446482.1 (PREDICTED: DNA-directed RNA polymerase 1B, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 222.2 bits (565), Expect = 3.0e-54
Identity = 111/133 (83.46%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRN+ KRAASRKATLFS+LSSS+SRKVTED+IFLDQIR SRSL++LN CCQSG SSRI 
Sbjct: 1   MWRNVLKRAASRKATLFSELSSSTSRKVTEDHIFLDQIRYSRSLETLNTCCQSGDSSRIS 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP+KVGFTNSNFPHSSNPVAFYGVL+HA+GYATAA+AAI EGDL G EEIQE+ME +S
Sbjct: 61  FLHPQKVGFTNSNFPHSSNPVAFYGVLNHAEGYATAAEAAISEGDLSGSEEIQEIMEGIS 120

Query: 136 KQDKVEPHFKKPR 149
           KQDKVEPHFKKP+
Sbjct: 121 KQDKVEPHFKKPK 133

BLAST of PI0006945 vs. NCBI nr
Match: KAA0034491.1 (DNA-directed RNA polymerase 1B [Cucumis melo var. makuwa] >TYK09045.1 DNA-directed RNA polymerase 1B [Cucumis melo var. makuwa])

HSP 1 Score: 222.2 bits (565), Expect = 3.0e-54
Identity = 111/133 (83.46%), Postives = 124/133 (93.23%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRN+ KRAASRKATLFS+LSSS+SRKVTED+IFLDQIR SRSL++LN CCQSG SSRI 
Sbjct: 1   MWRNVLKRAASRKATLFSELSSSTSRKVTEDHIFLDQIRYSRSLETLNTCCQSGDSSRIS 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP+KVGFTNSNFPHSSNPVAFYGVL+HA+GYATAA+AAI EGDL G EEIQE+ME +S
Sbjct: 61  FLHPQKVGFTNSNFPHSSNPVAFYGVLNHAEGYATAAEAAISEGDLSGSEEIQEIMEGIS 120

Query: 136 KQDKVEPHFKKPR 149
           KQDKVEPHFKKP+
Sbjct: 121 KQDKVEPHFKKPK 133

BLAST of PI0006945 vs. NCBI nr
Match: XP_004135424.3 (DNA-directed RNA polymerase 1B, mitochondrial [Cucumis sativus] >KAE8648775.1 hypothetical protein Csa_008524 [Cucumis sativus])

HSP 1 Score: 214.5 bits (545), Expect = 6.2e-52
Identity = 109/134 (81.34%), Postives = 121/134 (90.30%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRN+ K AASRKA LFS+ SSS+SRKVTED+IFLDQIRSSRSL++LN CCQSGGSSRI 
Sbjct: 1   MWRNVFKTAASRKAKLFSEFSSSTSRKVTEDHIFLDQIRSSRSLETLNTCCQSGGSSRII 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP+KVGFTNSNFPHSSNPV FYGVL HAKGYATAA+AAI EGDL G EEIQE+ME L+
Sbjct: 61  FLHPQKVGFTNSNFPHSSNPVPFYGVLYHAKGYATAAEAAIFEGDLSGSEEIQEIMEGLN 120

Query: 136 KQDKVEPHFKKPRG 150
           KQDKVE HFK+P+G
Sbjct: 121 KQDKVELHFKQPKG 134

BLAST of PI0006945 vs. NCBI nr
Match: XP_038892390.1 (DNA-directed RNA polymerase 1B, mitochondrial [Benincasa hispida])

HSP 1 Score: 172.6 bits (436), Expect = 2.7e-39
Identity = 91/133 (68.42%), Postives = 103/133 (77.44%), Query Frame = 0

Query: 16  MWRNICKRAASRKATLFSKLSSSSSRKVTEDYIFLDQIRSSRSLKSLNACCQSGGSSRIG 75
           MWRNI KR ASRK T FS+LSSS              IRSSR+L+SLN CCQS  S RI 
Sbjct: 1   MWRNIVKRIASRKTTFFSELSSS--------------IRSSRTLESLNTCCQSAVSRRIS 60

Query: 76  FLHPRKVGFTNSNFPHSSNPVAFYGVLDHAKGYATAAKAAIVEGDLLGFEEIQEMMEELS 135
           FLHP KVGFTNS+FPHSSNPVAFYGVL+HA GYATAA+AAI E DL G EEIQE++EELS
Sbjct: 61  FLHPHKVGFTNSSFPHSSNPVAFYGVLNHANGYATAAEAAISEEDLSGSEEIQELLEELS 119

Query: 136 KQDKVEPHFKKPR 149
           KQ+K+E HFK+P+
Sbjct: 121 KQEKIESHFKQPK 119

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7SYG21.4e-5483.46DNA-directed RNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BF591.4e-5483.46DNA-directed RNA polymerase OS=Cucumis melo OX=3656 GN=LOC103489205 PE=3 SV=1[more]
A0A1S3BF551.4e-5483.46DNA-directed RNA polymerase OS=Cucumis melo OX=3656 GN=LOC103489205 PE=3 SV=1[more]
A0A0A0KR043.0e-5281.34DNA-directed RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_5G607420 PE=3 SV=1[more]
A0A6J1HS834.7e-3766.17DNA-directed RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111467347 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_008446483.13.0e-5483.46PREDICTED: DNA-directed RNA polymerase 1B, mitochondrial isoform X2 [Cucumis mel... [more]
XP_008446482.13.0e-5483.46PREDICTED: DNA-directed RNA polymerase 1B, mitochondrial isoform X1 [Cucumis mel... [more]
KAA0034491.13.0e-5483.46DNA-directed RNA polymerase 1B [Cucumis melo var. makuwa] >TYK09045.1 DNA-direct... [more]
XP_004135424.36.2e-5281.34DNA-directed RNA polymerase 1B, mitochondrial [Cucumis sativus] >KAE8648775.1 hy... [more]
XP_038892390.12.7e-3968.42DNA-directed RNA polymerase 1B, mitochondrial [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0006945.1PI0006945.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006390 mitochondrial transcription
cellular_component GO:0034245 mitochondrial DNA-directed RNA polymerase complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity