PI0006678 (gene) Melon (PI 482460) v1

BLAST of PI0006678 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 6.2e-174
Identity = 362/686 (52.77%), Postives = 474/686 (69.10%), Query Frame = 0

Query: 72  SHLPPTANKPLKIPSSNGT-LTPRRSLKRSLLSRPKSRFGEQPRYIDSEDMFEENHESSR 131
           S L  + NKP + P+ N   LT R+S  RS+ S+PKSRF +    +D+  + EE     R
Sbjct: 52  SKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VR 111

Query: 132 EQIGA--TSSRSSALN------------TP-KAQPEEEDEEDIVKTEQLNKKHKKWKVKT 191
           EQ+GA  + SR+S  N            TP K   E++++E+I K  +LN++ +  K+ T
Sbjct: 112 EQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRS-KIST 171

Query: 192 M----------------INLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV- 251
           +                 +LT+N LK+  FWGL+VWKWC+L  VI  G++ T+W M ++ 
Sbjct: 172 LALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIV 231

Query: 252 ----------RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAIT 311
                     RKVLYFV+GLKKSVQV +WL L+LV W+ LFN    RS     K+L  IT
Sbjct: 232 FLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAAT-KVLKCIT 291

Query: 312 WTLVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAES 371
            TL+++L GA  WLVKTLLLKILA+ F+ + FFDRIQ+S+FH +VLQTL G PLM+ AE 
Sbjct: 292 RTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAER 351

Query: 372 AAK---FSCCRFSLKSKKS--DHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMS-I 431
             +        F+   KK     KKVIDMGK+H+++REKVSAWTM+VL+EAV +S +S I
Sbjct: 352 VGREPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTI 411

Query: 432 SQILDESYYNVG----DGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEI 491
           S  LDE+ Y  G    D EI  EME A   A  + RNVA P   +I+EEDLL+FM+KEE+
Sbjct: 412 SDTLDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEV 471

Query: 492 DLVLPNFE-VDETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITV 551
           DLV P F+   ET +I +KA   WVVKVY  R+ LAH+LNDTKTAVKQLN LVTA+L+ V
Sbjct: 472 DLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVV 531

Query: 552 TAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVD 611
           T VIWLLL+E+AT+KVL+F  +QL   AF+ G+ CK  FE+++FVFVMHP+DVGDRCVVD
Sbjct: 532 TVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVD 591

Query: 612 GVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLE 671
           GV +LVEEMN+LTTVFLKLNNEKVYYPN+VLATKPISNY+RSP+M ET+EFSI F+TP+ 
Sbjct: 592 GVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVS 651

Query: 672 RIGAMKEKIKRYLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRS 704
           +I  +KE+I  YLE+NPQHW P H +VVKEIEN+N++K+ALYS HT+ +Q+  E+  RR+
Sbjct: 652 KIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRT 711

BLAST of PI0006678 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 5.5e-154
Identity = 333/740 (45.00%), Postives = 467/740 (63.11%), Query Frame = 0

Query: 17  SQKEGENEVVVEISTSSVVSSKETRDRDDYSVLNQNRVDS---QNKQSTGSSIGHGYDSH 76
           +++   N   V I+ S    SK+ R    ++ L     D+   ++K     SI       
Sbjct: 2   AERRVSNGEEVVINVSDKEDSKDPRASPSFNPLASPDSDAGIEKSKPVPPISIPTPEIYK 61

Query: 77  LPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDSEDMFEENHESSREQI 136
              + +KP KIPS  G L  R+SL RS+ S+PKSRFGEQ  +       E    S REQ 
Sbjct: 62  FSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQF 121

Query: 137 GATS-----------SRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVK-------- 196
           GA S           +  S  +   A   +  EE+  + E++ KK K  +VK        
Sbjct: 122 GAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMKPLA 181

Query: 197 -------------TMINLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV--- 256
                         +++LT++ +     WGL+ WKWC+L  V L G++ T+W M+ V   
Sbjct: 182 FLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVVFI 241

Query: 257 --------RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWT 316
                   +KVLYFV+GLKK+VQV +W +LVL+ W+ LF+    R+ +T  + LD ITWT
Sbjct: 242 IEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKT-KRFLDFITWT 301

Query: 317 LVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAA 376
           +V+LL+G++L+LVKT  LK+LASKF+   FF+RIQES+FH +VLQTL G PL++ AE+  
Sbjct: 302 IVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAENVG 361

Query: 377 KF-SCCRFSL---KSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAV-TSSAMSISQI 436
           +  S    S    K  K   KKVIDMGK+H++++EKVSAWTM+VL+EAV TS   +IS  
Sbjct: 362 RVPSTGHLSFTRTKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSGISTISST 421

Query: 437 LDE--SYYNVGDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLP 496
           LDE  +     D EI +EME A   A  +  NVA P   +I+E+DLL+FM+KEE+DLVLP
Sbjct: 422 LDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKEEVDLVLP 481

Query: 497 NFEVDETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWL 556
             E  +T KI +K    WVV VY  RKT+ H+LNDTKTAVKQL+ L+T +L  +T ++W+
Sbjct: 482 LIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTVITFIVWM 541

Query: 557 LLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLV 616
           +L++IA++K+L+   SQ    AFM G+ CK  FE+ +FVFVMHP+DVGDRCVVDGV LLV
Sbjct: 542 VLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVVDGVMLLV 601

Query: 617 EEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMK 676
           EE+++LTTVFLK++NEKV+YPNSVL +KPISN+YRSPDM + ++F I F+TP E+IG +K
Sbjct: 602 EEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPAEKIGCLK 661

Query: 677 EKIKRYLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMEL 704
            KI  YL  N QHW P   ++V+ IEN+N++ + +   HT+N+Q Y EK  RR+ L++ +
Sbjct: 662 GKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLRRTALIIAI 721

BLAST of PI0006678 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 6.5e-115
Identity = 262/675 (38.81%), Postives = 396/675 (58.67%), Query Frame = 0

Query: 98  KRSLLSRPKSRFGEQPRYIDSEDMFEENHESSREQIGATSS----RSSALNTPKAQP-EE 157
           K+ +LSR K++   Q     +    ++    S  + G   S    +S    TP     EE
Sbjct: 185 KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEE 244

Query: 158 EDEEDIVKTEQLNKKHKKWKVKTMI----------------NLTVNRLKNCFFWGLKVWK 217
           E+EED    E L ++ K+ K+   +                +LT++ L+   +W L +WK
Sbjct: 245 EEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWK 304

Query: 218 WCLLATVILCGLIFTHWAMNVV-----------RKVLYFVYGLKKSVQVTLWLTLVLVTW 277
           W +   V++CG + + W + ++           ++VLYFVYG++KSVQ  LWL LVL+ W
Sbjct: 305 WEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAW 364

Query: 278 VSLFNRSNHRSSRTIGKILDAITWTLVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQ 337
             LF++   R +R+    L  +T  LV LL+  ++WLVKT+L+K+LAS FH   +FDRIQ
Sbjct: 365 HFLFDKKVERETRSTA--LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQ 424

Query: 338 ESIFHHHVLQTLLGSPLMQ----------------------GAE--SAAKFSCCRF---- 397
           ES+F  +V++TL G PLM+                      GA+   A K +   F    
Sbjct: 425 ESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVG 484

Query: 398 ------SLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMS-ISQILDESY 457
                  + SK+ +  + I + ++ ++  + VSAW MK L+  +   A+S + Q + ++ 
Sbjct: 485 KSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTT 544

Query: 458 YNVGDG-EIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFE-VD 517
               D   I  E E A   A KI  NV  PG ++I  ED L+F+ +EE +  +  FE   
Sbjct: 545 QEDEDATHIRSEYE-AKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGAS 604

Query: 518 ETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEI 577
           E+ KI+K  L+NWVVK ++ER+ LA  LNDTKTAV +L+ ++  ++  +  +IWLL++ I
Sbjct: 605 ESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGI 664

Query: 578 ATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 637
           AT++ L+ L SQL + AF+FGN+CKT FEA+IF+FVMHPFDVGDRC +DGVQL+VEEMNI
Sbjct: 665 ATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNI 724

Query: 638 LTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKR 697
           LTTVFL+ +N+K+ YPNSVL TKPI+NYYRSPDM + +EF +  ATP E+I A+K++I  
Sbjct: 725 LTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILS 784

Query: 698 YLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFE 704
           Y++    +W P  ++V   ++++N +KIA++ TH MN+QD GE+  RR  L+ E+ +   
Sbjct: 785 YVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCR 844

BLAST of PI0006678 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.5e-114
Identity = 268/702 (38.18%), Postives = 399/702 (56.84%), Query Frame = 0

Query: 71  DSHLPPTANKPLKIPSSNGTLTP-RRSLKRSLLSRPKSRFGEQPRYIDSEDMFEENHESS 130
           DS      N   ++   +G   P +RS    L  R +SR  + P    +  +  +  +  
Sbjct: 138 DSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPP----TPQLPPQTADMK 197

Query: 131 REQIGATSSRSSAL--NTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI---------- 190
             +I  +    S     +PK Q EEE E+D    E L ++++K K+   I          
Sbjct: 198 SGRIPKSGQMKSGFFGKSPKTQGEEE-EDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILI 257

Query: 191 ------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV-----------RK 250
                  L +  L+    W L++WKW  +  V++CG + + W + +V           ++
Sbjct: 258 IAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKR 317

Query: 251 VLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWTLVALLIGAVL 310
           VLYFVYG++K+VQ  LWL LVL+ W  LF+    +++ T  K L  +T   V LL+G +L
Sbjct: 318 VLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANT--KALRVVTKIFVCLLVGFLL 377

Query: 311 WLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAKFSCCRFSLK 370
           WLVKTLL+K+LAS FH   +FDRIQES+F  +V++TL G PL++  ++  +    R S++
Sbjct: 378 WLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEE--RISVE 437

Query: 371 SKK------------------------------------SDHKKVIDMGKIHQLQREKVS 430
            KK                                        K I +  +H+L  + VS
Sbjct: 438 VKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVS 497

Query: 431 AWTMKVLVEAVTSSAMSI--SQILDESYYNVGDGEIADEMEIASIVASKILRNVALPGKK 490
           AW MK L+  + + +++    Q+ D S  +    +I  E E A + A KI  NVA PG K
Sbjct: 498 AWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSK 557

Query: 491 FIQEEDLLKFMVKEEIDLVLPNFE-VDETRKINKKALRNWVVKVYQERKTLAHALNDTKT 550
           FI   D+++F+  +E    L  FE   ET +I+K +L+NWVV  ++ER+ LA  LNDTKT
Sbjct: 558 FIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKT 617

Query: 551 AVKQLNNLVTALLITVTAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIF 610
           AV +L+ +V  ++  +  VIWL+++ I ++K LV + SQ+ V AF+FGN CK  FE++I+
Sbjct: 618 AVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIY 677

Query: 611 VFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPD 670
           +FV+HPFDVGDRC +DGVQ++VEEMNILTTVFL+ +N+KV YPNS+L TK I NYYRSPD
Sbjct: 678 LFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPD 737

Query: 671 MSETIEFSIGFATPLERIGAMKEKIKRYLEKNPQHWRPNHLMVVKEIENVNEIKIALYST 704
           M + IEFSI   TP E+I  +K++I  Y+E    HW P  ++V K++E++N ++IA++ T
Sbjct: 738 MGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPT 797

BLAST of PI0006678 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 3.7e-110
Identity = 267/736 (36.28%), Postives = 396/736 (53.80%), Query Frame = 0

Query: 51  QNRVDSQNKQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLK---RSLLSRPKS 110
           QNRV        G S     D       ++ +K  S+  T+  +  +K   RS L  P +
Sbjct: 139 QNRVTLSPSVKDGDS---SEDEENRVDGSEVVKCTSNRSTMRTKTLMKMKTRSRLMDPPT 198

Query: 111 RFGEQPRYIDSEDMFEENHESSREQIGATSSRSSALNTPK--AQPEEEDEEDIVKTEQLN 170
                P Y    DM       S       S R++   TP      + E+EED    E L 
Sbjct: 199 -----PTY---PDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLP 258

Query: 171 KKHKKWKV----------------KTMINLTVNRLKNCFFWGLKVWKWCLLATVILCGLI 230
           +  +K K+                  + +L +  L+    W L +WKW ++  V++CG +
Sbjct: 259 EGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRL 318

Query: 231 FTHWAMNVV-----------RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSR 290
            + W + +            +KVLYFVYG++K VQ  LWL LVL+ W  LF++   R  R
Sbjct: 319 VSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMR 378

Query: 291 TIGKILDAITWTLVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLL 350
           +   +L  +T  L+ LL+  ++WL+KTLL+K+LAS FH   +FDRIQES+F  +V++TL 
Sbjct: 379 S--TVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLS 438

Query: 351 GSP--------------------------------------------LMQGAESAAKFSC 410
           G P                                             +Q + S    S 
Sbjct: 439 GPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSP 498

Query: 411 CRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQILDESYYNVG 470
                 SKK   ++ I +  + ++  + VSAW MK L+  +    +S    LDE   +  
Sbjct: 499 VLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLS---TLDEQIQDTT 558

Query: 471 DGE------IADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFE-V 530
             E      I  E E A + A KI +NVA PG ++I  ED ++F+ ++E +  +  FE  
Sbjct: 559 TQEDDKATQIRSEFE-AKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGA 618

Query: 531 DETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLME 590
            E  KI+K  L+NWVV  ++ER+ LA  LNDTKTAV +L+ +V  L+  V  +IWLL++ 
Sbjct: 619 SECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILG 678

Query: 591 IATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMN 650
           IAT+K L+ + SQL +  F+FGN+CKT FEA+IFVFVMHPFDVGDRC +DGVQ++VEEMN
Sbjct: 679 IATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMN 738

Query: 651 ILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIK 704
           ILTTVFL+ +N+K+ YPNS+L TKPI+NYYRSPDM + IEF +  ATP E+  A++++I 
Sbjct: 739 ILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRIL 798

BLAST of PI0006678 vs. ExPASy TrEMBL
Match: A0A5A7VG97 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001830 PE=3 SV=1)

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 629/736 (85.46%), Postives = 665/736 (90.35%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGEN--EVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQN 60
           MDVNGNKP+KVVRRSSSQKEGEN  +VVV+ISTSSVV  KETRD ++YSVLNQNRVDSQN
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDS 120
           K+STGSSI HGYDSH PPTAN+PLKIPSSN    PRRSLKRS+LSRPKSRFGEQPR+IDS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 EDMFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI- 180
           EDMFEENHES REQIGATSSR+SA NTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI 
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMIK 180

Query: 181 -------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV------ 240
                        +LTVNRLKNCFF GL+VWKWCLLATVI CGLIF  WA NV       
Sbjct: 181 WVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVAVSLIEG 240

Query: 241 -----RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWTLVA 300
                +KVLYFV+GLKKSVQ TLWL  VL TW  LFN+SNHRSSRT G+ILDAITWTLVA
Sbjct: 241 NCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSRTTGRILDAITWTLVA 300

Query: 301 LLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAKFS 360
           LLIG+ LWL+KTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPL++GAESAAKFS
Sbjct: 301 LLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEGAESAAKFS 360

Query: 361 CCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQILDESYYNV 420
            C FSL+S KSDHKK+IDMGKIHQLQREKVSAWTMKVLVEAV+SSAMSISQILDESYYNV
Sbjct: 361 RCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQILDESYYNV 420

Query: 421 GDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDETRKI 480
           GDGEI  EMEIAS+VASKILRNVALPGKKFIQEEDL KF+VKEEID VLP+FEVDETR+I
Sbjct: 421 GDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHFEVDETRRI 480

Query: 481 NKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIATSKV 540
            KKAL+ WVVKVYQERKTLAHAL DTKTAVKQLNNLVTA++I V  VIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKRYLEKN 660
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSET EFSI FATPLERIGAMKEKIK YLEKN
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEKIKSYLEKN 660

Query: 661 PQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFEELKIN 710
           PQHWRPNHL+VV+EIENVNEIKIAL+STHTM+YQ+YGEK+KRRSELVMELKRIFEEL IN
Sbjct: 661 PQHWRPNHLLVVREIENVNEIKIALFSTHTMSYQEYGEKLKRRSELVMELKRIFEELNIN 720

BLAST of PI0006678 vs. ExPASy TrEMBL
Match: A0A1S3BU27 (mechanosensitive ion channel protein 10-like OS=Cucumis melo OX=3656 GN=LOC103493692 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 5.1e-296
Identity = 555/652 (85.12%), Postives = 585/652 (89.72%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGEN--EVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQN 60
           MDVNGNKP+KVVRRSSSQKEGEN  +VVV+ISTSSVV  KETRD ++YSVLNQNRVDSQN
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDS 120
           K+STGSSI HGYDSH PPTAN+PLKIPSSN    PRRSLKRS+LSRPKSRFGEQPR+IDS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 EDMFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI- 180
           EDMFEENHES REQIGATSSR+SA NTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI 
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMIK 180

Query: 181 -------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV------ 240
                        +LTVNRLKNCFF GL+VWKWCLLATVI CGLIF  WA NV       
Sbjct: 181 WVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVAVSLIEG 240

Query: 241 -----RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWTLVA 300
                +KVLYFV+GLKKSVQ TLWL  VL TW  LFN+SNHRSSRT G+ILDAITWTLVA
Sbjct: 241 NCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSRTTGRILDAITWTLVA 300

Query: 301 LLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAKFS 360
           LLIG+ LWL+KTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPL++GAESAAKFS
Sbjct: 301 LLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEGAESAAKFS 360

Query: 361 CCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQILDESYYNV 420
            C FSL+S KSDHKK+IDMGKIHQLQREKVSAWTMKVLVEAV+SSAMSISQILDESYYNV
Sbjct: 361 RCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQILDESYYNV 420

Query: 421 GDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDETRKI 480
           GDGEI  EMEIAS+VASKILRNVALPGKKFIQEEDL KF+VKEEID VLP+FEVDETR+I
Sbjct: 421 GDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHFEVDETRRI 480

Query: 481 NKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIATSKV 540
            KKAL+ WVVKVYQERKTLAHAL DTKTAVKQLNNLVTA++I V  VIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEK 626
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSET EFSI FATPLERIGAMKEK
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEK 652

BLAST of PI0006678 vs. ExPASy TrEMBL
Match: A0A6J1C8Z8 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009404 PE=3 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 4.5e-284
Identity = 555/751 (73.90%), Postives = 616/751 (82.02%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGENEVVVEISTSSVVSSKETRDRDDYSVLNQNR-VDSQNK 60
           MDVNGNKPLK VRRSSSQKE EN   V +  S VV SKETRD +  SV +QNR VDSQNK
Sbjct: 1   MDVNGNKPLKAVRRSSSQKESENGGQVVVEISGVVPSKETRDENGCSVPSQNRVVDSQNK 60

Query: 61  QSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGE-QPRYIDS 120
             T SSIG         +ANKP KIP+S+GTL PR+SLKRS+ S+PKSRFGE QP YIDS
Sbjct: 61  GPTDSSIG---------SANKPPKIPNSSGTLMPRKSLKRSIQSKPKSRFGEQQPYYIDS 120

Query: 121 EDMFEENHESSREQIGATSSR------SSALNTPKAQPEEEDEEDIVKTEQLNK-KHKKW 180
            DMFEE+  S REQIGATSSR       SAL TP+AQ EEEDE+ I KTEQL+K KHKK 
Sbjct: 121 -DMFEEDRLSLREQIGATSSRRASPGHKSALTTPRAQAEEEDEDAIWKTEQLSKEKHKKL 180

Query: 181 KVKTMI--------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNV 240
           K+KT++              +LTVNRL+NCF WGL++WKWCLLATVILCG+IFT W MNV
Sbjct: 181 KMKTLVKWVGVFCIIGCLVASLTVNRLRNCFLWGLEIWKWCLLATVILCGMIFTKWVMNV 240

Query: 241 V-----------RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHR--SSRTIGKIL 300
           +           +KVLYFV+GLKK VQVTLWLTLVL TW SLF+RSNHR   S+T GKIL
Sbjct: 241 IVFLIERNFLLKKKVLYFVHGLKKIVQVTLWLTLVLATWASLFDRSNHRVLRSKTAGKIL 300

Query: 301 DAITWTLVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQ 360
           DA TWTLV+LLIGA LWLVKTLLLKILASKFH +RFFDRIQESIFHHHVLQTL   PLM 
Sbjct: 301 DAGTWTLVSLLIGAFLWLVKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLSRPPLMG 360

Query: 361 GAESA--AKFSCCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSI 420
            AE A  AK S  R SLK KKSDHKKVIDMGKIHQL+REKVSAWTMKVLV+AVTSS +SI
Sbjct: 361 VAECAASAKSSSGRLSLKGKKSDHKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSELSI 420

Query: 421 SQILDESYYN----VGDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEI 480
           SQ+LDESY N    V D +I DEME+A   A +I  NVALPG KFI+EEDLLKFM+KEE+
Sbjct: 421 SQLLDESYQNGGAEVADSDITDEMEVARAAAYRIFNNVALPGNKFIEEEDLLKFMIKEEV 480

Query: 481 DLVLPNFEVDETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVT 540
           DLVLP FEV +TR+I++KAL NWVVKVYQ RKTLAHAL DTKTAVKQLNNLVTALLI VT
Sbjct: 481 DLVLPLFEVADTRQIDRKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLVTALLIIVT 540

Query: 541 AVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG 600
           AV+WLLLMEIAT+KVLVFLLSQLAVAAFMFGN CKTTFEALIFVFVMHPFDVGDRCVVDG
Sbjct: 541 AVVWLLLMEIATTKVLVFLLSQLAVAAFMFGNTCKTTFEALIFVFVMHPFDVGDRCVVDG 600

Query: 601 VQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLER 660
           V LLVEEMNILTTVFLKL+NEKVYYPNSVL+TKPI+NYYRSPDMS+T+EFSI F TPLER
Sbjct: 601 VPLLVEEMNILTTVFLKLSNEKVYYPNSVLSTKPITNYYRSPDMSDTVEFSISFTTPLER 660

Query: 661 IGAMKEKIKRYLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSE 710
           IGAMKE+IKRYLEKN QHW PNH +VVKEIE+VN+IKIALY  HT+N+Q++ EK +RR+E
Sbjct: 661 IGAMKERIKRYLEKNSQHWHPNHDVVVKEIEDVNKIKIALYVNHTLNFQEWAEKNRRRTE 720

BLAST of PI0006678 vs. ExPASy TrEMBL
Match: A0A1S4DZ48 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=3 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 4.9e-283
Identity = 543/739 (73.48%), Postives = 606/739 (82.00%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGEN--EVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQN 60
           MDVNGNKP   +RRSSSQKE EN  EVVV++S             ++YSV  QNRVDSQ 
Sbjct: 1   MDVNGNKP---IRRSSSQKESENGGEVVVQLSGV----------ENEYSVPKQNRVDSQT 60

Query: 61  KQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDS 120
           K+ TGSS+G+G  S L PTANKP KIP SNGTLTPRRSL+RS LS+PKSRFGEQP YIDS
Sbjct: 61  KEPTGSSVGYGNASQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDS 120

Query: 121 EDMFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI- 180
           +   EENH S REQIGATSSRSS LNTPKAQPE ED  ++     LN+KHKK KVKT+  
Sbjct: 121 DMFEEENHVSLREQIGATSSRSSNLNTPKAQPEGEDGRNM----GLNEKHKKAKVKTVFK 180

Query: 181 -------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV------ 240
                        +LTV  LKN F WGLKVWKWCLLATVI CGLIFT W MNVV      
Sbjct: 181 WIGVFCIISCLVASLTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIER 240

Query: 241 -----RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNH--RSSRTIGKILDAITWTL 300
                +KVLYFV+GLKKSVQVTLWL+LVL TW SLF+R NH   SSR   KILDA+TWTL
Sbjct: 241 NFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTL 300

Query: 301 VALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAK 360
            +LLIGA LWL+KTLLLKILASKFH +RFFDRIQES+F HHVLQTLL  P ++  ES AK
Sbjct: 301 ASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVED-ESTAK 360

Query: 361 FSCCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQIL-DESY 420
           F CCRF  +SK+SD KKVIDMGKIHQL+REKVSAWTMKVLV+AVTSS MSISQIL DESY
Sbjct: 361 FRCCRFCFESKESDRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESY 420

Query: 421 YNVGDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDET 480
            +V DG+I +EM +A   A +I +NVALPG KFI+E DLL FM+ EE++LV P+FEVD+T
Sbjct: 421 RDVADGDITNEMNVAKEAAKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKT 480

Query: 481 RKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIAT 540
           RKI+ KAL NWVVKVYQ RKTLAHAL DTKTAVKQLNNL+TAL+I VTA+IWLLLMEIAT
Sbjct: 481 RKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIAT 540

Query: 541 SKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILT 600
           +KVLVFLL+QLAVAAFMFGN CKT FEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILT
Sbjct: 541 TKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILT 600

Query: 601 TVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKRYL 660
           TVFLKLNNEKVYYPNSVLATKPI+NYYRSPDM +T+EFSIGF TP+ERIGAMKE+IKRYL
Sbjct: 601 TVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYL 660

Query: 661 EKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFEEL 710
           E+NPQHW PNH +VVKEIENVN+IKIALY+ HTMN+QD+ EK +RR+ELVMELKRIFEEL
Sbjct: 661 EENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEEL 720

BLAST of PI0006678 vs. ExPASy TrEMBL
Match: A0A5D3D991 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001820 PE=3 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 4.9e-283
Identity = 543/739 (73.48%), Postives = 606/739 (82.00%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGEN--EVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQN 60
           MDVNGNKP   +RRSSSQKE EN  EVVV++S             ++YSV  QNRVDSQ 
Sbjct: 1   MDVNGNKP---IRRSSSQKESENGGEVVVQLSGV----------ENEYSVPKQNRVDSQT 60

Query: 61  KQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDS 120
           K+ TGSS+G+G  S L PTANKP KIP SNGTLTPRRSL+RS LS+PKSRFGEQP YIDS
Sbjct: 61  KEPTGSSVGYGNASQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDS 120

Query: 121 EDMFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI- 180
           +   EENH S REQIGATSSRSS LNTPKAQPE ED  ++     LN+KHKK KVKT+  
Sbjct: 121 DMFEEENHVSLREQIGATSSRSSNLNTPKAQPEGEDGRNM----GLNEKHKKAKVKTVFK 180

Query: 181 -------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV------ 240
                        +LTV  LKN F WGLKVWKWCLLATVI CGLIFT W MNVV      
Sbjct: 181 WIGVFCIISCLVASLTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIER 240

Query: 241 -----RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNH--RSSRTIGKILDAITWTL 300
                +KVLYFV+GLKKSVQVTLWL+LVL TW SLF+R NH   SSR   KILDA+TWTL
Sbjct: 241 NFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTL 300

Query: 301 VALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAK 360
            +LLIGA LWL+KTLLLKILASKFH +RFFDRIQES+F HHVLQTLL  P ++  ES AK
Sbjct: 301 ASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVED-ESTAK 360

Query: 361 FSCCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQIL-DESY 420
           F CCRF  +SK+SD KKVIDMGKIHQL+REKVSAWTMKVLV+AVTSS MSISQIL DESY
Sbjct: 361 FRCCRFCFESKESDRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESY 420

Query: 421 YNVGDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDET 480
            +V DG+I +EM +A   A +I +NVALPG KFI+E DLL FM+ EE++LV P+FEVD+T
Sbjct: 421 RDVADGDITNEMNVAKEAAKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKT 480

Query: 481 RKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIAT 540
           RKI+ KAL NWVVKVYQ RKTLAHAL DTKTAVKQLNNL+TAL+I VTA+IWLLLMEIAT
Sbjct: 481 RKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIAT 540

Query: 541 SKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILT 600
           +KVLVFLL+QLAVAAFMFGN CKT FEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILT
Sbjct: 541 TKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILT 600

Query: 601 TVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKRYL 660
           TVFLKLNNEKVYYPNSVLATKPI+NYYRSPDM +T+EFSIGF TP+ERIGAMKE+IKRYL
Sbjct: 601 TVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYL 660

Query: 661 EKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFEEL 710
           E+NPQHW PNH +VVKEIENVN+IKIALY+ HTMN+QD+ EK +RR+ELVMELKRIFEEL
Sbjct: 661 EENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEEL 720

BLAST of PI0006678 vs. NCBI nr
Match: KAA0064529.1 (mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa] >TYK20061.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 629/736 (85.46%), Postives = 665/736 (90.35%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGEN--EVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQN 60
           MDVNGNKP+KVVRRSSSQKEGEN  +VVV+ISTSSVV  KETRD ++YSVLNQNRVDSQN
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDS 120
           K+STGSSI HGYDSH PPTAN+PLKIPSSN    PRRSLKRS+LSRPKSRFGEQPR+IDS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 EDMFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI- 180
           EDMFEENHES REQIGATSSR+SA NTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI 
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMIK 180

Query: 181 -------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV------ 240
                        +LTVNRLKNCFF GL+VWKWCLLATVI CGLIF  WA NV       
Sbjct: 181 WVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVAVSLIEG 240

Query: 241 -----RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWTLVA 300
                +KVLYFV+GLKKSVQ TLWL  VL TW  LFN+SNHRSSRT G+ILDAITWTLVA
Sbjct: 241 NCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSRTTGRILDAITWTLVA 300

Query: 301 LLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAKFS 360
           LLIG+ LWL+KTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPL++GAESAAKFS
Sbjct: 301 LLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEGAESAAKFS 360

Query: 361 CCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQILDESYYNV 420
            C FSL+S KSDHKK+IDMGKIHQLQREKVSAWTMKVLVEAV+SSAMSISQILDESYYNV
Sbjct: 361 RCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQILDESYYNV 420

Query: 421 GDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDETRKI 480
           GDGEI  EMEIAS+VASKILRNVALPGKKFIQEEDL KF+VKEEID VLP+FEVDETR+I
Sbjct: 421 GDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHFEVDETRRI 480

Query: 481 NKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIATSKV 540
            KKAL+ WVVKVYQERKTLAHAL DTKTAVKQLNNLVTA++I V  VIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKRYLEKN 660
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSET EFSI FATPLERIGAMKEKIK YLEKN
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEKIKSYLEKN 660

Query: 661 PQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFEELKIN 710
           PQHWRPNHL+VV+EIENVNEIKIAL+STHTM+YQ+YGEK+KRRSELVMELKRIFEEL IN
Sbjct: 661 PQHWRPNHLLVVREIENVNEIKIALFSTHTMSYQEYGEKLKRRSELVMELKRIFEELNIN 720

BLAST of PI0006678 vs. NCBI nr
Match: XP_004141432.2 (mechanosensitive ion channel protein 10 [Cucumis sativus])

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 626/736 (85.05%), Postives = 662/736 (89.95%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGEN--EVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQN 60
           MDVNGNKP KVVRRSSSQKEGEN  +VVVEIS SSVV SKETRD ++YSVL Q+RVDSQN
Sbjct: 1   MDVNGNKP-KVVRRSSSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60

Query: 61  KQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDS 120
           K+STGSSI HG+DSH+PPTAN+PLKIPSSN TLTPRRSLKRS+LSRPKSRFGEQ RY DS
Sbjct: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120

Query: 121 EDMFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI- 180
           +D FEE HES REQ GATSSRSS+LNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKT+I 
Sbjct: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTVIK 180

Query: 181 -------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV------ 240
                        +LTVNRLKNCFF GL++WKWCLLATVI CGLI THWAMNVV      
Sbjct: 181 WIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEG 240

Query: 241 -----RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWTLVA 300
                +KVLYFV+GLKKSVQVTLWL  VL TW  LFN+ NHRSSRT GKILDAITWTLVA
Sbjct: 241 NFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVA 300

Query: 301 LLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAKFS 360
           LLIG+ LWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQ LLG PLMQ  ESAAKFS
Sbjct: 301 LLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFS 360

Query: 361 CCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQILDESYYNV 420
            C FS ++KKSD KK+ID GKIH LQREKVS+WTMKVLVEAVTSSAMSISQILDESYYNV
Sbjct: 361 RCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNV 420

Query: 421 GDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDETRKI 480
            DGEI  EMEIAS+VASKILRNVALPGKKFIQEEDLL+F+VKEEIDLVLP+FEVDET++I
Sbjct: 421 DDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRI 480

Query: 481 NKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIATSKV 540
            KKAL+ WVVKV+QERKTLAHAL DTKTAVKQLNNLVTA++I V AVIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGV LLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVPLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKRYLEKN 660
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSET EFSI FATPLERIGAMKEKIKRYLEKN
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKN 660

Query: 661 PQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFEELKIN 710
           PQHWRP+HLMVVKEIENVNEIKIALYSTHTM+YQDYGEK+KRRSELVMELKRIFEELKIN
Sbjct: 661 PQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKIN 720

BLAST of PI0006678 vs. NCBI nr
Match: KAE8649918.1 (hypothetical protein Csa_012502 [Cucumis sativus])

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 623/734 (84.88%), Postives = 660/734 (89.92%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGEN--EVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQN 60
           MDVNGNKP KVVRRSSSQKEGEN  +VVVEIS SSVV SKETRD ++YSVL Q+RVDSQN
Sbjct: 1   MDVNGNKP-KVVRRSSSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60

Query: 61  KQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDS 120
           K+STGSSI HG+DSH+PPTAN+PLKIPSSN TLTPRRSLKRS+LSRPKSRFGEQ RY DS
Sbjct: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120

Query: 121 EDMFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI- 180
           +D FEE HES REQ GATSSRSS+LNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKT+I 
Sbjct: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTVIK 180

Query: 181 -------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV------ 240
                        +LTVNRLKNCFF GL++WKWCLLATVI CGLI THWAMNVV      
Sbjct: 181 WIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEG 240

Query: 241 -----RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWTLVA 300
                +KVLYFV+GLKKSVQVTLWL  VL TW  LFN+ NHRSSRT GKILDAITWTLVA
Sbjct: 241 NFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVA 300

Query: 301 LLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAKFS 360
           LLIG+ LWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQ LLG PLMQ  ESAAKFS
Sbjct: 301 LLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFS 360

Query: 361 CCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQILDESYYNV 420
            C FS ++KKSD KK+ID GKIH LQREKVS+WTMKVLVEAVTSSAMSISQILDESYYNV
Sbjct: 361 RCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNV 420

Query: 421 GDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDETRKI 480
            DGEI  EMEIAS+VASKILRNVALPGKKFIQEEDLL+F+VKEEIDLVLP+FEVDET++I
Sbjct: 421 DDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRI 480

Query: 481 NKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIATSKV 540
            KKAL+ WVVKV+QERKTLAHAL DTKTAVKQLNNLVTA++I V AVIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGV LLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVPLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKRYLEKN 660
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSET EFSI FATPLERIGAMKEKIKRYLEKN
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKN 660

Query: 661 PQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFEELKIN 708
           PQHWRP+HLMVVKEIENVNEIKIALYSTHTM+YQDYGEK+KRRSELVMELKRIFEELKIN
Sbjct: 661 PQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKIN 720

BLAST of PI0006678 vs. NCBI nr
Match: XP_038897085.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 594/733 (81.04%), Postives = 635/733 (86.63%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGENEVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQNKQ 60
           MDVNGN P K VRRSSS KE ENE  V +  SSV+SSKET+D + YSV  QNRVDSQ K+
Sbjct: 1   MDVNGNNPSKAVRRSSSLKESENEGHVVVEISSVLSSKETKDENGYSVTKQNRVDSQTKE 60

Query: 61  STGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDSED 120
            T SSIGH  DS+LPP AN+PLKIP SNGTLT RRSLKRS LSRPKSRFGEQPRY DS D
Sbjct: 61  PTDSSIGHSNDSYLPPIANEPLKIPYSNGTLTLRRSLKRSYLSRPKSRFGEQPRYTDS-D 120

Query: 121 MFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI--- 180
           MFEEN  S REQIGATSSRSS LN P AQ EEED EDIVKTE+ NKK KK KVKT+I   
Sbjct: 121 MFEENDVSLREQIGATSSRSSVLNPPNAQSEEEDGEDIVKTEKSNKKDKKVKVKTLIKLV 180

Query: 181 -----------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV-------- 240
                      +LTVNRLKNCFFWGLKVWKWCLLATVI CG IFT W M+VV        
Sbjct: 181 GAFCLIACLVASLTVNRLKNCFFWGLKVWKWCLLATVIFCGKIFTTWVMHVVVNLIERNF 240

Query: 241 ---RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWTLVALL 300
              + VLYFV+GL+KSV+VTLWLTLVLVTW SLFN +NHRSSRT+GKILDAITWTLVALL
Sbjct: 241 LLKKNVLYFVHGLRKSVRVTLWLTLVLVTWASLFNWNNHRSSRTVGKILDAITWTLVALL 300

Query: 301 IGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAKFSCC 360
           IGAVLWLVKTLLLKILASKFHK+RFFDRIQESIFHHHVLQTLL  PL +GAE  AKFSCC
Sbjct: 301 IGAVLWLVKTLLLKILASKFHKNRFFDRIQESIFHHHVLQTLLKCPLKEGAEGTAKFSCC 360

Query: 361 RFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQILDESYYNVGD 420
           RFSLKSKKSDHKKVID GKIHQLQREKVSAWT+KVL+EAVTSS MSISQILDES  NV D
Sbjct: 361 RFSLKSKKSDHKKVIDTGKIHQLQREKVSAWTIKVLIEAVTSSQMSISQILDES-SNVAD 420

Query: 421 GEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDETRKINK 480
           GEI DEMEIA  VA KI +N+A  GKKFIQEEDLLKFMVKEEIDLVLP+FEVDETRKI+K
Sbjct: 421 GEITDEMEIAIAVACKIFKNIA-HGKKFIQEEDLLKFMVKEEIDLVLPHFEVDETRKIDK 480

Query: 481 KALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIATSKVLV 540
           KAL NWVVKVYQERKTLAHALNDTKTAV++LNNL  AL+I VTAVIWLLLMEIATSKVLV
Sbjct: 481 KALINWVVKVYQERKTLAHALNDTKTAVEELNNLAIALIIIVTAVIWLLLMEIATSKVLV 540

Query: 541 FLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVFLK 600
           FLLSQLAVAAFMFGNACKT FEALIFVFVMHPFDVGD CVVDGVQLLVEEMNILTTVFLK
Sbjct: 541 FLLSQLAVAAFMFGNACKTAFEALIFVFVMHPFDVGDFCVVDGVQLLVEEMNILTTVFLK 600

Query: 601 LNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKRYLEKNPQ 660
           LNNEKVYYPNSVLATKPI+NYYRSP+M +TIEFSI FATPLERIGAMKEKIKRYLEKNPQ
Sbjct: 601 LNNEKVYYPNSVLATKPITNYYRSPNMFDTIEFSISFATPLERIGAMKEKIKRYLEKNPQ 660

Query: 661 HWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFEELKINYN 709
           HW PNH +VVKEIENVN+IKIAL++ HTMN+QD+ EK +RR+ELVMELKRIFEELKINYN
Sbjct: 661 HWHPNHSVVVKEIENVNKIKIALFAKHTMNFQDWSEKNRRRTELVMELKRIFEELKINYN 720

BLAST of PI0006678 vs. NCBI nr
Match: XP_008452774.1 (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo])

HSP 1 Score: 1026.5 bits (2653), Expect = 1.1e-295
Identity = 555/652 (85.12%), Postives = 585/652 (89.72%), Query Frame = 0

Query: 1   MDVNGNKPLKVVRRSSSQKEGEN--EVVVEISTSSVVSSKETRDRDDYSVLNQNRVDSQN 60
           MDVNGNKP+KVVRRSSSQKEGEN  +VVV+ISTSSVV  KETRD ++YSVLNQNRVDSQN
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KQSTGSSIGHGYDSHLPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDS 120
           K+STGSSI HGYDSH PPTAN+PLKIPSSN    PRRSLKRS+LSRPKSRFGEQPR+IDS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 EDMFEENHESSREQIGATSSRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI- 180
           EDMFEENHES REQIGATSSR+SA NTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMI 
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMIK 180

Query: 181 -------------NLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV------ 240
                        +LTVNRLKNCFF GL+VWKWCLLATVI CGLIF  WA NV       
Sbjct: 181 WVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVAVSLIEG 240

Query: 241 -----RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWTLVA 300
                +KVLYFV+GLKKSVQ TLWL  VL TW  LFN+SNHRSSRT G+ILDAITWTLVA
Sbjct: 241 NCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSRTTGRILDAITWTLVA 300

Query: 301 LLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAAKFS 360
           LLIG+ LWL+KTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPL++GAESAAKFS
Sbjct: 301 LLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEGAESAAKFS 360

Query: 361 CCRFSLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMSISQILDESYYNV 420
            C FSL+S KSDHKK+IDMGKIHQLQREKVSAWTMKVLVEAV+SSAMSISQILDESYYNV
Sbjct: 361 RCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQILDESYYNV 420

Query: 421 GDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFEVDETRKI 480
           GDGEI  EMEIAS+VASKILRNVALPGKKFIQEEDL KF+VKEEID VLP+FEVDETR+I
Sbjct: 421 GDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHFEVDETRRI 480

Query: 481 NKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEIATSKV 540
            KKAL+ WVVKVYQERKTLAHAL DTKTAVKQLNNLVTA++I V  VIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEK 626
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSET EFSI FATPLERIGAMKEK
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEK 652

BLAST of PI0006678 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 612.5 bits (1578), Expect = 4.4e-175
Identity = 362/686 (52.77%), Postives = 474/686 (69.10%), Query Frame = 0

Query: 72  SHLPPTANKPLKIPSSNGT-LTPRRSLKRSLLSRPKSRFGEQPRYIDSEDMFEENHESSR 131
           S L  + NKP + P+ N   LT R+S  RS+ S+PKSRF +    +D+  + EE     R
Sbjct: 52  SKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VR 111

Query: 132 EQIGA--TSSRSSALN------------TP-KAQPEEEDEEDIVKTEQLNKKHKKWKVKT 191
           EQ+GA  + SR+S  N            TP K   E++++E+I K  +LN++ +  K+ T
Sbjct: 112 EQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRS-KIST 171

Query: 192 M----------------INLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV- 251
           +                 +LT+N LK+  FWGL+VWKWC+L  VI  G++ T+W M ++ 
Sbjct: 172 LALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIV 231

Query: 252 ----------RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAIT 311
                     RKVLYFV+GLKKSVQV +WL L+LV W+ LFN    RS     K+L  IT
Sbjct: 232 FLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAAT-KVLKCIT 291

Query: 312 WTLVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAES 371
            TL+++L GA  WLVKTLLLKILA+ F+ + FFDRIQ+S+FH +VLQTL G PLM+ AE 
Sbjct: 292 RTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAER 351

Query: 372 AAK---FSCCRFSLKSKKS--DHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMS-I 431
             +        F+   KK     KKVIDMGK+H+++REKVSAWTM+VL+EAV +S +S I
Sbjct: 352 VGREPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTI 411

Query: 432 SQILDESYYNVG----DGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEI 491
           S  LDE+ Y  G    D EI  EME A   A  + RNVA P   +I+EEDLL+FM+KEE+
Sbjct: 412 SDTLDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEV 471

Query: 492 DLVLPNFE-VDETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITV 551
           DLV P F+   ET +I +KA   WVVKVY  R+ LAH+LNDTKTAVKQLN LVTA+L+ V
Sbjct: 472 DLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVV 531

Query: 552 TAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVD 611
           T VIWLLL+E+AT+KVL+F  +QL   AF+ G+ CK  FE+++FVFVMHP+DVGDRCVVD
Sbjct: 532 TVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVD 591

Query: 612 GVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLE 671
           GV +LVEEMN+LTTVFLKLNNEKVYYPN+VLATKPISNY+RSP+M ET+EFSI F+TP+ 
Sbjct: 592 GVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVS 651

Query: 672 RIGAMKEKIKRYLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRS 704
           +I  +KE+I  YLE+NPQHW P H +VVKEIEN+N++K+ALYS HT+ +Q+  E+  RR+
Sbjct: 652 KIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRT 711

BLAST of PI0006678 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 612.5 bits (1578), Expect = 4.4e-175
Identity = 362/686 (52.77%), Postives = 474/686 (69.10%), Query Frame = 0

Query: 72  SHLPPTANKPLKIPSSNGT-LTPRRSLKRSLLSRPKSRFGEQPRYIDSEDMFEENHESSR 131
           S L  + NKP + P+ N   LT R+S  RS+ S+PKSRF +    +D+  + EE     R
Sbjct: 52  SKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VR 111

Query: 132 EQIGA--TSSRSSALN------------TP-KAQPEEEDEEDIVKTEQLNKKHKKWKVKT 191
           EQ+GA  + SR+S  N            TP K   E++++E+I K  +LN++ +  K+ T
Sbjct: 112 EQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRS-KIST 171

Query: 192 M----------------INLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV- 251
           +                 +LT+N LK+  FWGL+VWKWC+L  VI  G++ T+W M ++ 
Sbjct: 172 LALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIV 231

Query: 252 ----------RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAIT 311
                     RKVLYFV+GLKKSVQV +WL L+LV W+ LFN    RS     K+L  IT
Sbjct: 232 FLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAAT-KVLKCIT 291

Query: 312 WTLVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAES 371
            TL+++L GA  WLVKTLLLKILA+ F+ + FFDRIQ+S+FH +VLQTL G PLM+ AE 
Sbjct: 292 RTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAER 351

Query: 372 AAK---FSCCRFSLKSKKS--DHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMS-I 431
             +        F+   KK     KKVIDMGK+H+++REKVSAWTM+VL+EAV +S +S I
Sbjct: 352 VGREPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTI 411

Query: 432 SQILDESYYNVG----DGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEI 491
           S  LDE+ Y  G    D EI  EME A   A  + RNVA P   +I+EEDLL+FM+KEE+
Sbjct: 412 SDTLDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEV 471

Query: 492 DLVLPNFE-VDETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITV 551
           DLV P F+   ET +I +KA   WVVKVY  R+ LAH+LNDTKTAVKQLN LVTA+L+ V
Sbjct: 472 DLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVV 531

Query: 552 TAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVD 611
           T VIWLLL+E+AT+KVL+F  +QL   AF+ G+ CK  FE+++FVFVMHP+DVGDRCVVD
Sbjct: 532 TVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVD 591

Query: 612 GVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLE 671
           GV +LVEEMN+LTTVFLKLNNEKVYYPN+VLATKPISNY+RSP+M ET+EFSI F+TP+ 
Sbjct: 592 GVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVS 651

Query: 672 RIGAMKEKIKRYLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRS 704
           +I  +KE+I  YLE+NPQHW P H +VVKEIEN+N++K+ALYS HT+ +Q+  E+  RR+
Sbjct: 652 KIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRT 711

BLAST of PI0006678 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 612.5 bits (1578), Expect = 4.4e-175
Identity = 362/686 (52.77%), Postives = 474/686 (69.10%), Query Frame = 0

Query: 72  SHLPPTANKPLKIPSSNGT-LTPRRSLKRSLLSRPKSRFGEQPRYIDSEDMFEENHESSR 131
           S L  + NKP + P+ N   LT R+S  RS+ S+PKSRF +    +D+  + EE     R
Sbjct: 52  SKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VR 111

Query: 132 EQIGA--TSSRSSALN------------TP-KAQPEEEDEEDIVKTEQLNKKHKKWKVKT 191
           EQ+GA  + SR+S  N            TP K   E++++E+I K  +LN++ +  K+ T
Sbjct: 112 EQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRS-KIST 171

Query: 192 M----------------INLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV- 251
           +                 +LT+N LK+  FWGL+VWKWC+L  VI  G++ T+W M ++ 
Sbjct: 172 LALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIV 231

Query: 252 ----------RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAIT 311
                     RKVLYFV+GLKKSVQV +WL L+LV W+ LFN    RS     K+L  IT
Sbjct: 232 FLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAAT-KVLKCIT 291

Query: 312 WTLVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAES 371
            TL+++L GA  WLVKTLLLKILA+ F+ + FFDRIQ+S+FH +VLQTL G PLM+ AE 
Sbjct: 292 RTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAER 351

Query: 372 AAK---FSCCRFSLKSKKS--DHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMS-I 431
             +        F+   KK     KKVIDMGK+H+++REKVSAWTM+VL+EAV +S +S I
Sbjct: 352 VGREPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTI 411

Query: 432 SQILDESYYNVG----DGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEI 491
           S  LDE+ Y  G    D EI  EME A   A  + RNVA P   +I+EEDLL+FM+KEE+
Sbjct: 412 SDTLDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEV 471

Query: 492 DLVLPNFE-VDETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITV 551
           DLV P F+   ET +I +KA   WVVKVY  R+ LAH+LNDTKTAVKQLN LVTA+L+ V
Sbjct: 472 DLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVV 531

Query: 552 TAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVD 611
           T VIWLLL+E+AT+KVL+F  +QL   AF+ G+ CK  FE+++FVFVMHP+DVGDRCVVD
Sbjct: 532 TVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVD 591

Query: 612 GVQLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLE 671
           GV +LVEEMN+LTTVFLKLNNEKVYYPN+VLATKPISNY+RSP+M ET+EFSI F+TP+ 
Sbjct: 592 GVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVS 651

Query: 672 RIGAMKEKIKRYLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRS 704
           +I  +KE+I  YLE+NPQHW P H +VVKEIEN+N++K+ALYS HT+ +Q+  E+  RR+
Sbjct: 652 KIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRT 711

BLAST of PI0006678 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 546.2 bits (1406), Expect = 3.9e-155
Identity = 333/740 (45.00%), Postives = 467/740 (63.11%), Query Frame = 0

Query: 17  SQKEGENEVVVEISTSSVVSSKETRDRDDYSVLNQNRVDS---QNKQSTGSSIGHGYDSH 76
           +++   N   V I+ S    SK+ R    ++ L     D+   ++K     SI       
Sbjct: 2   AERRVSNGEEVVINVSDKEDSKDPRASPSFNPLASPDSDAGIEKSKPVPPISIPTPEIYK 61

Query: 77  LPPTANKPLKIPSSNGTLTPRRSLKRSLLSRPKSRFGEQPRYIDSEDMFEENHESSREQI 136
              + +KP KIPS  G L  R+SL RS+ S+PKSRFGEQ  +       E    S REQ 
Sbjct: 62  FSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQF 121

Query: 137 GATS-----------SRSSALNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVK-------- 196
           GA S           +  S  +   A   +  EE+  + E++ KK K  +VK        
Sbjct: 122 GAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMKPLA 181

Query: 197 -------------TMINLTVNRLKNCFFWGLKVWKWCLLATVILCGLIFTHWAMNVV--- 256
                         +++LT++ +     WGL+ WKWC+L  V L G++ T+W M+ V   
Sbjct: 182 FLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFVVFI 241

Query: 257 --------RKVLYFVYGLKKSVQVTLWLTLVLVTWVSLFNRSNHRSSRTIGKILDAITWT 316
                   +KVLYFV+GLKK+VQV +W +LVL+ W+ LF+    R+ +T  + LD ITWT
Sbjct: 242 IEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKT-KRFLDFITWT 301

Query: 317 LVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLMQGAESAA 376
           +V+LL+G++L+LVKT  LK+LASKF+   FF+RIQES+FH +VLQTL G PL++ AE+  
Sbjct: 302 IVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAENVG 361

Query: 377 KF-SCCRFSL---KSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAV-TSSAMSISQI 436
           +  S    S    K  K   KKVIDMGK+H++++EKVSAWTM+VL+EAV TS   +IS  
Sbjct: 362 RVPSTGHLSFTRTKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSGISTISST 421

Query: 437 LDE--SYYNVGDGEIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLP 496
           LDE  +     D EI +EME A   A  +  NVA P   +I+E+DLL+FM+KEE+DLVLP
Sbjct: 422 LDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKEEVDLVLP 481

Query: 497 NFEVDETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWL 556
             E  +T KI +K    WVV VY  RKT+ H+LNDTKTAVKQL+ L+T +L  +T ++W+
Sbjct: 482 LIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTVITFIVWM 541

Query: 557 LLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLV 616
           +L++IA++K+L+   SQ    AFM G+ CK  FE+ +FVFVMHP+DVGDRCVVDGV LLV
Sbjct: 542 VLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVVDGVMLLV 601

Query: 617 EEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMK 676
           EE+++LTTVFLK++NEKV+YPNSVL +KPISN+YRSPDM + ++F I F+TP E+IG +K
Sbjct: 602 EEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPAEKIGCLK 661

Query: 677 EKIKRYLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMEL 704
            KI  YL  N QHW P   ++V+ IEN+N++ + +   HT+N+Q Y EK  RR+ L++ +
Sbjct: 662 GKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLRRTALIIAI 721

BLAST of PI0006678 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 416.4 bits (1069), Expect = 4.6e-116
Identity = 262/675 (38.81%), Postives = 396/675 (58.67%), Query Frame = 0

Query: 98  KRSLLSRPKSRFGEQPRYIDSEDMFEENHESSREQIGATSS----RSSALNTPKAQP-EE 157
           K+ +LSR K++   Q     +    ++    S  + G   S    +S    TP     EE
Sbjct: 185 KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEE 244

Query: 158 EDEEDIVKTEQLNKKHKKWKVKTMI----------------NLTVNRLKNCFFWGLKVWK 217
           E+EED    E L ++ K+ K+   +                +LT++ L+   +W L +WK
Sbjct: 245 EEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWK 304

Query: 218 WCLLATVILCGLIFTHWAMNVV-----------RKVLYFVYGLKKSVQVTLWLTLVLVTW 277
           W +   V++CG + + W + ++           ++VLYFVYG++KSVQ  LWL LVL+ W
Sbjct: 305 WEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAW 364

Query: 278 VSLFNRSNHRSSRTIGKILDAITWTLVALLIGAVLWLVKTLLLKILASKFHKDRFFDRIQ 337
             LF++   R +R+    L  +T  LV LL+  ++WLVKT+L+K+LAS FH   +FDRIQ
Sbjct: 365 HFLFDKKVERETRSTA--LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQ 424

Query: 338 ESIFHHHVLQTLLGSPLMQ----------------------GAE--SAAKFSCCRF---- 397
           ES+F  +V++TL G PLM+                      GA+   A K +   F    
Sbjct: 425 ESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVG 484

Query: 398 ------SLKSKKSDHKKVIDMGKIHQLQREKVSAWTMKVLVEAVTSSAMS-ISQILDESY 457
                  + SK+ +  + I + ++ ++  + VSAW MK L+  +   A+S + Q + ++ 
Sbjct: 485 KSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTT 544

Query: 458 YNVGDG-EIADEMEIASIVASKILRNVALPGKKFIQEEDLLKFMVKEEIDLVLPNFE-VD 517
               D   I  E E A   A KI  NV  PG ++I  ED L+F+ +EE +  +  FE   
Sbjct: 545 QEDEDATHIRSEYE-AKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGAS 604

Query: 518 ETRKINKKALRNWVVKVYQERKTLAHALNDTKTAVKQLNNLVTALLITVTAVIWLLLMEI 577
           E+ KI+K  L+NWVVK ++ER+ LA  LNDTKTAV +L+ ++  ++  +  +IWLL++ I
Sbjct: 605 ESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGI 664

Query: 578 ATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 637
           AT++ L+ L SQL + AF+FGN+CKT FEA+IF+FVMHPFDVGDRC +DGVQL+VEEMNI
Sbjct: 665 ATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNI 724

Query: 638 LTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETIEFSIGFATPLERIGAMKEKIKR 697
           LTTVFL+ +N+K+ YPNSVL TKPI+NYYRSPDM + +EF +  ATP E+I A+K++I  
Sbjct: 725 LTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILS 784

Query: 698 YLEKNPQHWRPNHLMVVKEIENVNEIKIALYSTHTMNYQDYGEKIKRRSELVMELKRIFE 704
           Y++    +W P  ++V   ++++N +KIA++ TH MN+QD GE+  RR  L+ E+ +   
Sbjct: 785 YVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCR 844