PI0006542 (gene) Melon (PI 482460) v1

Overview


Name	PI0006542
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	E3 ubiquitin-protein ligase RMA3
Location	chr07: 22086103 .. 22088789 (+)
RNA-Seq Expression	PI0006542
Synteny	PI0006542

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGTTCTTCCTCAAATTCTCTCCGTCAACATTTTCTTGATTTGATTTTGGTCGGAGATTGTCGTACGAAACCTTTCAGTTTCTTATTTAACGGTGAGTGTTTTTTTTTTTTTTTTTTTTTTTTAATGTTGATAATTTCTAATGTTACGTTTGGTTTGATTTTTTGTTTGAATTTGTGTTATGATTTTGAAGGAAATGTGTTAGGTGAATTGGGGTTTTGTTTGAATGAATGGGTATTTCTTTCTTTTTAAGAAAACGAGCGCTGGGTTTTGATCTATGGGTTATTGGGCTTTTGGTGATTTTGTTCTGACTTCATTTTAAACTGCTGCCCTTTTCTTATTTCGTTGTGATTCGATCTGGGTTGATTTTGCGGAGAAATTCTATTTTTATGGCGTTTGGTTTGTTTCCATTTTTTGTTTTTCCGCCTCAAGAAAATCTTTGAAGTTTCGAGATTTCTTCTAAAGACTTGCTTCCGATCATACACGTTTCTCTCTGTTCGCAATTTAGAGGATAAGTAGCGTAAACTACAAGCTGAAGGGGTCGATATTTTGGACGTCGATTCGACATTTTGATTGTTATTTATTTGGGATTCTTGCATTGTTGCTCTGCAATTGGTTTGCTAGCTTATGTGGTTACCATCTTTTGCCCAAAAATAAATGTTTTTCTTTGCCCCTTTCTTTATAGAGGGTGTTGTGAGCCACGATTGTGGTTTGAGACAGCTGGTTTCTTAATGGTCTGAATATTTGTATTTTACATTCATTGTTTGAGTTTATCTGTAGAAGTTTCTCAATGCAAGTCATTGGTATCTTACATTCAGCATTGATCCTCCCAGGTCTTGTGTCTATTGATCGACCCTTGAATCTTTGCTATCTGTGTTTTCTTTTAAGTTTTCAGTCCTTTTGGTTTCTTTTGGTTGGTAGTGGACAGAACAGAACTTTAGTTTTGTATACAGTTGTGACATCTTGTTCCTTAGTCCTCGATCAATAACCATTTCGTTTTTTAAAATTGTGCTTCTTTTCTAACATGAGAGCCCTCCAAGGAAACACTGCCAAATTCCAAAATCAGAAACAAAATTTTATAAATTACTCTTTTAAGTTTTCAAAACTTTCCTTGGATTTTGAAAACAAAGTTTTGAAAACATTCCTACAAAATCTATAACAATAGATAACAAAACATAGAATCTCAAAGTGTAAGAATTGTTTATGATCTTAGTTTTAAAAAAACTAAATGATTATTAAATGGGGCCGTTTATAAATGTTTCAATATTGATTACCAGTTGTTGGTGGTTGAATATTTGATTTTTATTGACTTCGTCTAATTAAAGTGATCTTTATCCTCTTGATTAGCCTCCCTGGGGAAAGTTCGTTGTTTGATCCTTCTTCCTTTGTGTGAAAGCTTCTGTCTTTCCTTGAAATTTGTTTTGCTTTTTTCTAATCCTTTTTTTTTTTTTTTTTTTTTGAGAAAACAACTGGCATAAGTGGCAATTTTATTGTATTTGCATTATTGCAAATGGGACATTGTTATTTGTGTCCAAATTGGTATCTGTCTATAATGTTCTTCAATCGTTTATAGCAGCTGATTTTATTCTTTAAGTTACTTATTTTCCGAGAATCTCATTGTCTTTTTCTTCTTTTAAACAGAACGCATCCCTCTTTCCATGGAACAAAACTACTCTGTACCTGAGGCATACTTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAATCTCAGAAGACGCAAATGGTTGCTTCGATTGCAACATTTGCTTAGACTCAGCAGCTGACCCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGCATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACGAACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACTCCCTCCTCGTTGGTTCCCCTCTATGGGCGTGGCACGTCGAACTCAGATTCCGAATCCAAGAAATCTCACTTGGGTATGGCTGTACCTCGAAGACCACCACCGTCAATGAACACACCACCCCATTCCAATTCATCCTCGGCATTGTATCCTAGCCAGGAGCTTCATTCAAATTATATCCGCTCGCCTTCACACCCGATCTATCACCAACAATATTTCCCCCAGGCCTACGGCAACTTTGCCTCATACGCACCATCTTATCTTGGTAATGCCGTGATAACGAGTCTGCTAAACCCAACTATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATATTTGGTAGCGTGGATGGGAACTTATTGCCATACCCGCCATACAACAACTCGATCTCAGGAAATGCCAGTACGAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATATGTCTTCTGTTATTTTGAAGTTGGGAATAGATGTTGTGTGGAGAAGCATATGATGATATACATACAGATAAGCAGCTAGAATTTTGGTAGATGACTTTACTGTGAGAAGTCATCTAATATGTACAGAAGGTTAAAGCTAATATCACATATTGTTTTGTAACTCCTTTTGGTGCTTTTGTGTGTATTGGCGTGTTTATAATACTTCCCTACGTTTTCCCACCCCCTAGAAGTCTAATAAATCATCGCCAAATAATATCAAGTCTAA

mRNA sequence

ATTGTTCTTCCTCAAATTCTCTCCGTCAACATTTTCTTGATTTGATTTTGGTCGGAGATTGTCGTACGAAACCTTTCAGTTTCTTATTTAACGAACGCATCCCTCTTTCCATGGAACAAAACTACTCTGTACCTGAGGCATACTTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAATCTCAGAAGACGCAAATGGTTGCTTCGATTGCAACATTTGCTTAGACTCAGCAGCTGACCCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGCATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACGAACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACTCCCTCCTCGTTGGTTCCCCTCTATGGGCGTGGCACGTCGAACTCAGATTCCGAATCCAAGAAATCTCACTTGGGTATGGCTGTACCTCGAAGACCACCACCGTCAATGAACACACCACCCCATTCCAATTCATCCTCGGCATTGTATCCTAGCCAGGAGCTTCATTCAAATTATATCCGCTCGCCTTCACACCCGATCTATCACCAACAATATTTCCCCCAGGCCTACGGCAACTTTGCCTCATACGCACCATCTTATCTTGGTAATGCCGTGATAACGAGTCTGCTAAACCCAACTATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATATTTGGTAGCGTGGATGGGAACTTATTGCCATACCCGCCATACAACAACTCGATCTCAGGAAATGCCAGTACGAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATATGTCTTCTGTTATTTTGAAGTTGGGAATAGATGTTGTGTGGAGAAGCATATGATGATATACATACAGATAAGCAGCTAGAATTTTGGTAGATGACTTTACTGTGAGAAGTCATCTAATATGTACAGAAGGTTAAAGCTAATATCACATATTGTTTTGTAACTCCTTTTGGTGCTTTTGTGTGTATTGGCGTGTTTATAATACTTCCCTACGTTTTCCCACCCCCTAGAAGTCTAATAAATCATCGCCAAATAATATCAAGTCTAA

Coding sequence (CDS)

ATGGAACAAAACTACTCTGTACCTGAGGCATACTTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAATCTCAGAAGACGCAAATGGTTGCTTCGATTGCAACATTTGCTTAGACTCAGCAGCTGACCCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGCATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACGAACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACTCCCTCCTCGTTGGTTCCCCTCTATGGGCGTGGCACGTCGAACTCAGATTCCGAATCCAAGAAATCTCACTTGGGTATGGCTGTACCTCGAAGACCACCACCGTCAATGAACACACCACCCCATTCCAATTCATCCTCGGCATTGTATCCTAGCCAGGAGCTTCATTCAAATTATATCCGCTCGCCTTCACACCCGATCTATCACCAACAATATTTCCCCCAGGCCTACGGCAACTTTGCCTCATACGCACCATCTTATCTTGGTAATGCCGTGATAACGAGTCTGCTAAACCCAACTATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATATTTGGTAGCGTGGATGGGAACTTATTGCCATACCCGCCATACAACAACTCGATCTCAGGAAATGCCAGTACGAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATATGTCTTCTGTTATTTTGA

Protein sequence

MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF

Homology

[Close]

BLAST of PI0006542 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 4.3e-48
Identity = 120/265 (45.28%), Postives = 163/265 (61.51%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISE-DANGCFDCNICLDSAADPVVTLCG 60
           ME N+ +      +  +  + +   +++T  T  + + +GCFDCNICLD+A DPVVTLCG
Sbjct: 1   MEGNFFIRSDAQRAHDNGFIAKQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVTLCG 60

Query: 61  HLYCWPCIYKWLHVQISS-NEPENTQNCPVCKASITPSSLVPLYGRGTSNSDS---ESKK 120
           HL+CWPCIYKWLHVQ+SS +  ++  NCPVCK++IT +SLVPLYGRG S+  S     K+
Sbjct: 61  HLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGRGMSSPSSTFGSKKQ 120

Query: 121 SHLGMAVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFAS 180
             L   +PRRP PS    P   S+S+L PS +   +   SPS   ++ QY P+    F +
Sbjct: 121 DALSTDIPRRPAPSALRNP-ITSASSLNPSLQ---HQTLSPS--FHNHQYSPR---GFTT 180

Query: 181 YAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQE 240
              + L NAV+ S L P IGMFG+ V+TRIFG+          + N+I   A     ++ 
Sbjct: 181 TESTDLANAVMMSFLYPVIGMFGDLVYTRIFGT----------FTNTI---AQPYQSQRM 240

Query: 241 MQLDKSLNRVSIFLFCCFIICLLLF 261
           MQ +KSLNRVSIF  CC I+CLLLF
Sbjct: 241 MQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of PI0006542 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 6.0e-42
Identity = 101/241 (41.91%), Postives = 137/241 (56.85%), Query Frame = 0


Query: 24  WKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNEPENT 83
           WKS++ +  + E+ +G FDCNICLD   +PV+TLCGHLYCWPCIYKW++ Q  S+E  + 
Sbjct: 24  WKSMNDE--VEENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQ 83

Query: 84  Q--NCPVCKASITPSSLVPLYGR-GTSNSDSESKKSHLGMAVPRRPPPSMNTPPHSNSSS 143
           Q   CPVCKA ++  +L+PLYGR G S   SE K  +LG+ +P+RPP      P      
Sbjct: 84  QQPQCPVCKAEVSEKTLIPLYGRGGQSTKPSEGKAPNLGIVIPQRPP-----SPRCGGHF 143

Query: 144 ALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSYLGNAVITSLLNPTIGMFGET 203
            L  +    S  ++   +    Q   P   G++ S      G A    L +    M GE 
Sbjct: 144 LLPTTDSNPSQLLQRRGYQQQSQTRQPAYQGSYMSSPMLSPGGATANMLQH---SMIGEV 203

Query: 204 VFTRIFGSVDGNLLPYP-PYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLL 261
            + RIFG+    +  YP  YN +IS  +S RMRRQ  Q D+SL R+  FLFCCF+ CL+L
Sbjct: 204 AYARIFGNSSTTMYTYPNSYNLAIS--SSPRMRRQLSQADRSLGRICFFLFCCFVTCLIL 252

[Close]

BLAST of PI0006542 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.7e-39
Identity = 103/240 (42.92%), Postives = 145/240 (60.42%), Query Frame = 0


Query: 26  SISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQ--ISSNEPENT 85
           ++S  S  ++D+N  FDCNICLDS  +PVVTLCGHL+CWPCI+KWL VQ   +S+E +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 86  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPP---SMNTPPHSNSSS 145
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P    +S+
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 146 ALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSYLGNAVITSLLNPTIGMFGET 205
            L  SQ +H N   SP      + Y+P + G  +S + SY      +++L+P + M GE 
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS-GVMSSNSLSY------SAVLDPVMVMVGEM 212

Query: 206 VFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 261
           V TR+FG+   +   YP   N ++G +  RMRR+ MQ DKSL R+  F  CC ++CLLLF
Sbjct: 213 VATRLFGTRVMDRFAYPDTYN-LAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of PI0006542 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.0e-28
Identity = 79/232 (34.05%), Postives = 107/232 (46.12%), Query Frame = 0


Query: 36  DANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNE-------PENTQNCPV 95
           D+ G FDCNICLD   DPVVTLCGHL+CWPCI+KW +   +S +             CPV
Sbjct: 14  DSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPV 73

Query: 96  CKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTPPHSNSSSALYPSQEL 155
           CK+ ++ ++LVP+YGRG        K    G  VP R                       
Sbjct: 74  CKSDVSEATLVPIYGRG-------QKAPQSGSNVPSR----------------------- 133

Query: 156 HSNYIRSPSHPIYHQQYFPQAYGNFASYAPSYLGNAVITSLLNPTIGMFGETVFTRIFGS 215
                  P+ P+Y  +   Q  G   S    Y        + +P +G+  E V+ R+FG 
Sbjct: 134 -------PTGPVYDLRGVGQRLGEGESQRYMY-------RMPDPVMGVVCEMVYRRLFGE 193

Query: 216 VDGNLLPYPPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 261
              N+ PY   N         R RR+ MQ ++SL+RV +FL C   +CL LF
Sbjct: 194 SSSNMAPYRDMN--------VRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of PI0006542 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.2e-19
Identity = 47/104 (45.19%), Postives = 65/104 (62.50%), Query Frame = 0


Query: 31  STISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCK 90
           S+ ++D +  F+CNICLD+A D VV+LCGHL+CWPC+ +WL  +       N Q CPVCK
Sbjct: 14  SSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTR------PNNQVCPVCK 73

Query: 91  ASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTPP 135
           ++I  + +VP+YGRG  +SD   K       VP RP    + PP
Sbjct: 74  SAIDGNKVVPIYGRGGDSSDPRKK-------VPPRPKGQRSEPP 104

[Close]

BLAST of PI0006542 vs. ExPASy TrEMBL
Match: A0A5A7VD79 (E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003900 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.0e-145
Identity = 257/260 (98.85%), Postives = 258/260 (99.23%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSIST+STISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTESTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180
           AVPRRPPPSMNTPP SNSSSA YPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY
Sbjct: 121 AVPRRPPPSMNTPPPSNSSSASYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180

Query: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240
           LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK
Sbjct: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240

Query: 241 SLNRVSIFLFCCFIICLLLF 261
           SLNRVSIFLFCCFIICLLLF
Sbjct: 241 SLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of PI0006542 vs. ExPASy TrEMBL
Match: A0A1S3BTX0 (E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo OX=3656 GN=LOC103493490 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.0e-145
Identity = 257/260 (98.85%), Postives = 258/260 (99.23%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSIST+STISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTESTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180
           AVPRRPPPSMNTPP SNSSSA YPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY
Sbjct: 121 AVPRRPPPSMNTPPPSNSSSASYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180

Query: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240
           LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK
Sbjct: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240

Query: 241 SLNRVSIFLFCCFIICLLLF 261
           SLNRVSIFLFCCFIICLLLF
Sbjct: 241 SLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of PI0006542 vs. ExPASy TrEMBL
Match: A0A0A0KZN0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637750 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.2e-143
Identity = 256/261 (98.08%), Postives = 257/261 (98.47%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQA-YGNFASYAPS 180
           AVPRRPPPSMNTP HSNSSS LYPSQELHSNYIRSPSHPIYHQQYFPQA YGNFASY+PS
Sbjct: 121 AVPRRPPPSMNTPSHSNSSSTLYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLD 240
           YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPY PYNNSISGNASTRMRRQEMQLD
Sbjct: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 261
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 261

[Close]

BLAST of PI0006542 vs. ExPASy TrEMBL
Match: A0A6J1J1I0 (E3 ubiquitin-protein ligase RMA3-like OS=Cucurbita maxima OX=3661 GN=LOC111480472 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.0e-129
Identity = 224/260 (86.15%), Postives = 241/260 (92.69%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           M+Q YS  EAYLESE DVSLKQNWKS+S QST+SEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MDQTYSASEAYLESEHDVSLKQNWKSMSAQSTVSEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           L+CWPCIYKW HVQISSNE ENT NCPVCKASIT SSLVPLYGRG SNSDSESKKSHLG+
Sbjct: 61  LFCWPCIYKWFHVQISSNETENTHNCPVCKASITLSSLVPLYGRGMSNSDSESKKSHLGI 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180
            +PRRPPPSMNTPPHSN++SAL+PS+ELH NYIRSP H IYHQQYFPQAYGNFA YAPSY
Sbjct: 121 TIPRRPPPSMNTPPHSNAASALHPSEELHPNYIRSPLHRIYHQQYFPQAYGNFAPYAPSY 180

Query: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240
           LGNA++TSLLNPTIG+ GETV+TRIFGS DGNLLPYPPYN+S++GN  TRMRRQEMQLDK
Sbjct: 181 LGNAMVTSLLNPTIGLIGETVYTRIFGSADGNLLPYPPYNHSVAGNIGTRMRRQEMQLDK 240

Query: 241 SLNRVSIFLFCCFIICLLLF 261
           SLNRVSIFLFCCFIICLLLF
Sbjct: 241 SLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of PI0006542 vs. ExPASy TrEMBL
Match: A0A6J1FI46 (E3 ubiquitin-protein ligase RMA3-like OS=Cucurbita moschata OX=3662 GN=LOC111445968 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.5e-128
Identity = 224/260 (86.15%), Postives = 241/260 (92.69%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           M+Q YS PEAYLESEQDVSLKQNWKS+S QST+SEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MDQTYSSPEAYLESEQDVSLKQNWKSMSAQSTVSEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           L+CWPCIYKW HVQISS E ENT NCPVCKASITPSSLVPLYGRG S SDSESKKSHLG+
Sbjct: 61  LFCWPCIYKWFHVQISSIETENTHNCPVCKASITPSSLVPLYGRGKSYSDSESKKSHLGI 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180
            VPRRPPPSMNTPPHSN++SAL+PS+ELH NYIRSP H IYHQQYFPQAYGNFA YAPSY
Sbjct: 121 TVPRRPPPSMNTPPHSNAASALHPSEELHPNYIRSPLHRIYHQQYFPQAYGNFAPYAPSY 180

Query: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240
           LGNA++TSLLNPTIG+ GETV+TRIFGS DG+LLPYPPYN+ ++GN  TRMRRQEMQLDK
Sbjct: 181 LGNAMVTSLLNPTIGLIGETVYTRIFGSADGSLLPYPPYNHLVAGNVGTRMRRQEMQLDK 240

Query: 241 SLNRVSIFLFCCFIICLLLF 261
           SLNRVSIFLFCCFIICLLLF
Sbjct: 241 SLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of PI0006542 vs. NCBI nr
Match: XP_008452467.1 (PREDICTED: E3 ubiquitin-protein ligase RMA3 [Cucumis melo] >KAA0064336.1 E3 ubiquitin-protein ligase RMA3 [Cucumis melo var. makuwa] >TYK20251.1 E3 ubiquitin-protein ligase RMA3 [Cucumis melo var. makuwa])

HSP 1 Score: 524.2 bits (1349), Expect = 6.2e-145
Identity = 257/260 (98.85%), Postives = 258/260 (99.23%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSIST+STISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTESTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180
           AVPRRPPPSMNTPP SNSSSA YPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY
Sbjct: 121 AVPRRPPPSMNTPPPSNSSSASYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180

Query: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240
           LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK
Sbjct: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240

Query: 241 SLNRVSIFLFCCFIICLLLF 261
           SLNRVSIFLFCCFIICLLLF
Sbjct: 241 SLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of PI0006542 vs. NCBI nr
Match: XP_004141226.1 (E3 ubiquitin-protein ligase RMA3 [Cucumis sativus] >KGN55135.1 hypothetical protein Csa_012256 [Cucumis sativus])

HSP 1 Score: 518.1 bits (1333), Expect = 4.5e-143
Identity = 256/261 (98.08%), Postives = 257/261 (98.47%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQA-YGNFASYAPS 180
           AVPRRPPPSMNTP HSNSSS LYPSQELHSNYIRSPSHPIYHQQYFPQA YGNFASY+PS
Sbjct: 121 AVPRRPPPSMNTPSHSNSSSTLYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLD 240
           YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPY PYNNSISGNASTRMRRQEMQLD
Sbjct: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 261
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 261

[Close]

BLAST of PI0006542 vs. NCBI nr
Match: XP_038899693.1 (E3 ubiquitin-protein ligase RMA3 [Benincasa hispida])

HSP 1 Score: 508.8 bits (1309), Expect = 2.7e-140
Identity = 248/264 (93.94%), Postives = 256/264 (96.97%), Query Frame = 0


Query: 1   MEQNYSVP----EAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVT 60
           MEQNYSVP    EAYLESEQDVSLKQNWKSISTQST+SEDANGCFDCNICLDSAA+PVVT
Sbjct: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120
           LCGHLYCWPCIYKWLHVQISSN PENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS
Sbjct: 61  LCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120

Query: 121 HLGMAVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASY 180
           HLGM VPRRPPPSMNTPPHSN++SAL+PSQELH NY+RSPSHPIYHQQYFPQAYG+FASY
Sbjct: 121 HLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASY 180

Query: 181 APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEM 240
           APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNN+I GNASTRMRRQEM
Sbjct: 181 APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQEM 240

Query: 241 QLDKSLNRVSIFLFCCFIICLLLF 261
           QLDKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 QLDKSLNRVSIFLFCCFIICLLLF 264

[Close]

BLAST of PI0006542 vs. NCBI nr
Match: XP_023521850.1 (E3 ubiquitin-protein ligase RMA3-like [Cucurbita pepo subsp. pepo] >XP_023521858.1 E3 ubiquitin-protein ligase RMA3-like [Cucurbita pepo subsp. pepo] >KAG6607875.1 E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037404.1 E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 476.5 bits (1225), Expect = 1.5e-130
Identity = 227/260 (87.31%), Postives = 243/260 (93.46%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           M+Q YS PEAYLESEQDVSLKQNWKS+S QST+SEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MDQTYSSPEAYLESEQDVSLKQNWKSMSAQSTVSEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           L+CWPCIYKW HVQISSNE ENT NCPVCKASITPSSLVPLYGRG S SDSESKKSHLG+
Sbjct: 61  LFCWPCIYKWFHVQISSNETENTHNCPVCKASITPSSLVPLYGRGKSYSDSESKKSHLGI 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180
            VPRRPPPSMNTPPHSN++SAL+PS+ELH NYIRSP H IYHQQYFPQAYGNFA YAPSY
Sbjct: 121 TVPRRPPPSMNTPPHSNAASALHPSEELHPNYIRSPLHRIYHQQYFPQAYGNFAPYAPSY 180

Query: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240
           LGNA++TSLLNPTIG+ GETV+TRIFGS DGNLLPYPPYN+S++GN  TRMRRQEMQLDK
Sbjct: 181 LGNAMVTSLLNPTIGLIGETVYTRIFGSADGNLLPYPPYNHSVAGNVGTRMRRQEMQLDK 240

Query: 241 SLNRVSIFLFCCFIICLLLF 261
           SLNRVSIFLFCCFIICLLLF
Sbjct: 241 SLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of PI0006542 vs. NCBI nr
Match: XP_022981299.1 (E3 ubiquitin-protein ligase RMA3-like [Cucurbita maxima])

HSP 1 Score: 470.7 bits (1210), Expect = 8.2e-129
Identity = 224/260 (86.15%), Postives = 241/260 (92.69%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           M+Q YS  EAYLESE DVSLKQNWKS+S QST+SEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MDQTYSASEAYLESEHDVSLKQNWKSMSAQSTVSEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           L+CWPCIYKW HVQISSNE ENT NCPVCKASIT SSLVPLYGRG SNSDSESKKSHLG+
Sbjct: 61  LFCWPCIYKWFHVQISSNETENTHNCPVCKASITLSSLVPLYGRGMSNSDSESKKSHLGI 120

Query: 121 AVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSY 180
            +PRRPPPSMNTPPHSN++SAL+PS+ELH NYIRSP H IYHQQYFPQAYGNFA YAPSY
Sbjct: 121 TIPRRPPPSMNTPPHSNAASALHPSEELHPNYIRSPLHRIYHQQYFPQAYGNFAPYAPSY 180

Query: 181 LGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDK 240
           LGNA++TSLLNPTIG+ GETV+TRIFGS DGNLLPYPPYN+S++GN  TRMRRQEMQLDK
Sbjct: 181 LGNAMVTSLLNPTIGLIGETVYTRIFGSADGNLLPYPPYNHSVAGNIGTRMRRQEMQLDK 240

Query: 241 SLNRVSIFLFCCFIICLLLF 261
           SLNRVSIFLFCCFIICLLLF
Sbjct: 241 SLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of PI0006542 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 193.0 bits (489), Expect = 3.1e-49
Identity = 120/265 (45.28%), Postives = 163/265 (61.51%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISE-DANGCFDCNICLDSAADPVVTLCG 60
           ME N+ +      +  +  + +   +++T  T  + + +GCFDCNICLD+A DPVVTLCG
Sbjct: 1   MEGNFFIRSDAQRAHDNGFIAKQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVTLCG 60

Query: 61  HLYCWPCIYKWLHVQISS-NEPENTQNCPVCKASITPSSLVPLYGRGTSNSDS---ESKK 120
           HL+CWPCIYKWLHVQ+SS +  ++  NCPVCK++IT +SLVPLYGRG S+  S     K+
Sbjct: 61  HLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGRGMSSPSSTFGSKKQ 120

Query: 121 SHLGMAVPRRPPPSMNTPPHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFAS 180
             L   +PRRP PS    P   S+S+L PS +   +   SPS   ++ QY P+    F +
Sbjct: 121 DALSTDIPRRPAPSALRNP-ITSASSLNPSLQ---HQTLSPS--FHNHQYSPR---GFTT 180

Query: 181 YAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQE 240
              + L NAV+ S L P IGMFG+ V+TRIFG+          + N+I   A     ++ 
Sbjct: 181 TESTDLANAVMMSFLYPVIGMFGDLVYTRIFGT----------FTNTI---AQPYQSQRM 240

Query: 241 MQLDKSLNRVSIFLFCCFIICLLLF 261
           MQ +KSLNRVSIF  CC I+CLLLF
Sbjct: 241 MQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of PI0006542 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 163.3 bits (412), Expect = 2.6e-40
Identity = 103/240 (42.92%), Postives = 145/240 (60.42%), Query Frame = 0


Query: 26  SISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQ--ISSNEPENT 85
           ++S  S  ++D+N  FDCNICLDS  +PVVTLCGHL+CWPCI+KWL VQ   +S+E +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 86  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPP---SMNTPPHSNSSS 145
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P    +S+
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 146 ALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSYLGNAVITSLLNPTIGMFGET 205
            L  SQ +H N   SP      + Y+P + G  +S + SY      +++L+P + M GE 
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS-GVMSSNSLSY------SAVLDPVMVMVGEM 212

Query: 206 VFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 261
           V TR+FG+   +   YP   N ++G +  RMRR+ MQ DKSL R+  F  CC ++CLLLF
Sbjct: 213 VATRLFGTRVMDRFAYPDTYN-LAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of PI0006542 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 163.3 bits (412), Expect = 2.6e-40
Identity = 103/240 (42.92%), Postives = 145/240 (60.42%), Query Frame = 0


Query: 26  SISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQ--ISSNEPENT 85
           ++S  S  ++D+N  FDCNICLDS  +PVVTLCGHL+CWPCI+KWL VQ   +S+E +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 86  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPP---SMNTPPHSNSSS 145
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P    +S+
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 146 ALYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSYLGNAVITSLLNPTIGMFGET 205
            L  SQ +H N   SP      + Y+P + G  +S + SY      +++L+P + M GE 
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS-GVMSSNSLSY------SAVLDPVMVMVGEM 212

Query: 206 VFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 261
           V TR+FG+   +   YP   N ++G +  RMRR+ MQ DKSL R+  F  CC ++CLLLF
Sbjct: 213 VATRLFGTRVMDRFAYPDTYN-LAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of PI0006542 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 126.3 bits (316), Expect = 3.5e-29
Identity = 79/232 (34.05%), Postives = 107/232 (46.12%), Query Frame = 0


Query: 36  DANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNE-------PENTQNCPV 95
           D+ G FDCNICLD   DPVVTLCGHL+CWPCI+KW +   +S +             CPV
Sbjct: 14  DSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPV 73

Query: 96  CKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTPPHSNSSSALYPSQEL 155
           CK+ ++ ++LVP+YGRG        K    G  VP R                       
Sbjct: 74  CKSDVSEATLVPIYGRG-------QKAPQSGSNVPSR----------------------- 133

Query: 156 HSNYIRSPSHPIYHQQYFPQAYGNFASYAPSYLGNAVITSLLNPTIGMFGETVFTRIFGS 215
                  P+ P+Y  +   Q  G   S    Y        + +P +G+  E V+ R+FG 
Sbjct: 134 -------PTGPVYDLRGVGQRLGEGESQRYMY-------RMPDPVMGVVCEMVYRRLFGE 193

Query: 216 VDGNLLPYPPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 261
              N+ PY   N         R RR+ MQ ++SL+RV +FL C   +CL LF
Sbjct: 194 SSSNMAPYRDMN--------VRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of PI0006542 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 6.9e-25
Identity = 80/241 (33.20%), Postives = 115/241 (47.72%), Query Frame = 0


Query: 26  SISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNEPENTQN 85
           S S  +  + D  G F+CNIC + A DP+VTLCGHL+CWPC+Y+WLH         ++Q 
Sbjct: 11  SYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLH------HHSHSQE 70

Query: 86  CPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRP----PPSMNTPPHSNSSSA 145
           CPVCKA +    LVPLYGRG + +D  SK+ + G+ +P RP    P +   PP   ++S 
Sbjct: 71  CPVCKAVVQDDKLVPLYGRGKNQTDPRSKR-YPGLRIPNRPTGQRPETAAPPPQPEAASN 130

Query: 146 LYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASYAPSYLGNAVITSLLNPTIGMFGETV 205
            +       NY       I        A   F +++  + G  ++ SL N     F +  
Sbjct: 131 FF-------NYGIGLMGGIMPM----MATTRFGNFSMGFGG--LLPSLFNFQFHGFHDAT 190

Query: 206 FTRIFGSVDGNLLPYPPYNNSISG----NASTRMRRQEMQLDKSLNRVSIFLFCCFIICL 259
              ++GS  G   PY  Y+N   G         M R   Q D  L  +  F+  C +I L
Sbjct: 191 ---LYGSTPG--YPYGGYHNGFRGVPPRGQERPMARGGNQSDAFLKNILFFVGICVVIFL 226

The following BLAST results are available for this feature:

BLAST of PI0006542 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GUK7	4.3e-48	45.28	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
Q6R567	6.0e-42	41.91	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
O64425	3.7e-39	42.92	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
P93030	5.0e-28	34.05	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q09463	3.2e-19	45.19	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]

BLAST of PI0006542 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VD79	3.0e-145	98.85	E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BTX0	3.0e-145	98.85	E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo OX=3656 GN=LOC103493490 PE=4 SV...	[more]
A0A0A0KZN0	2.2e-143	98.08	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637750 P...	[more]
A0A6J1J1I0	4.0e-129	86.15	E3 ubiquitin-protein ligase RMA3-like OS=Cucurbita maxima OX=3661 GN=LOC11148047...	[more]
A0A6J1FI46	1.5e-128	86.15	E3 ubiquitin-protein ligase RMA3-like OS=Cucurbita moschata OX=3662 GN=LOC111445...	[more]

BLAST of PI0006542 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008452467.1	6.2e-145	98.85	PREDICTED: E3 ubiquitin-protein ligase RMA3 [Cucumis melo] >KAA0064336.1 E3 ubiq...	[more]
XP_004141226.1	4.5e-143	98.08	E3 ubiquitin-protein ligase RMA3 [Cucumis sativus] >KGN55135.1 hypothetical prot...	[more]
XP_038899693.1	2.7e-140	93.94	E3 ubiquitin-protein ligase RMA3 [Benincasa hispida]	[more]
XP_023521850.1	1.5e-130	87.31	E3 ubiquitin-protein ligase RMA3-like [Cucurbita pepo subsp. pepo] >XP_023521858...	[more]
XP_022981299.1	8.2e-129	86.15	E3 ubiquitin-protein ligase RMA3-like [Cucurbita maxima]	[more]

BLAST of PI0006542 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27470.1	3.1e-49	45.28	RING membrane-anchor 3	[more]
AT4G03510.1	2.6e-40	42.92	RING membrane-anchor 1	[more]
AT4G03510.2	2.6e-40	42.92	RING membrane-anchor 1	[more]
AT4G28270.1	3.5e-29	34.05	RING membrane-anchor 2	[more]
AT2G23780.1	6.9e-25	33.20	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 43..89 e-value: 6.6E-10 score: 48.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 43..90 score: 12.872856
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 43..89 e-value: 1.1E-8 score: 34.8
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 3..106 e-value: 9.6E-18 score: 65.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 104..142
None	No IPR available	PANTHER	PTHR12313:SF69	E3 UBIQUITIN-PROTEIN LIGASE RMA3-LIKE	coord: 2..260
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 41..91 e-value: 1.86718E-25 score: 93.1375
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 23..96
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..260
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 58..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0006542.1	PI0006542.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity