PI0006148 (gene) Melon (PI 482460) v1

Overview
NamePI0006148
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr01: 14637089 .. 14637862 (-)
RNA-Seq ExpressionPI0006148
SyntenyPI0006148
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGATTATAGGCCTATTTCGTGTTGTAATGTGGTTTACAAGTGCATTTCTAAGATTTTGGCGGATAGGCTTCATGTGTGGCTTCCTTTTTTTATCGGTGGTAATCAGTCGGCCTTTGTAGTTGGGAGGAGTATTGTTGATAACATTCTGCTTTGTCAGGAGATGGTGGGGATTATCATGGTTCTTCGGGTTCGCCTAGGTGTGCAATGAACGTGGATCTTCAGAAGGCTTATGATTCGGTGAATTGGGATTTTCTGTTCAGCTTGTTGCTTGCTATTGGCACTCCCCTGCATTTTGTGAGTTGGATTAGGGCGTGTGTTACTTCTCCTATGTTCTCTATCATGGTTAATGGTTCTGTCGAGGAGTTTTATCATGGTAGAAAAGGGTTGCGGCAGGGTGACCCCTTATCCCCCTTTTTGTTTGTGATGGTGATGGAGGTGCTTACTCATCTTTTGAATAGGCCCTCTCTAGATTTTAAGTATCATCATCTTTGTGAGAAGGTTTGTTTGACATCTTATGATTTTTTGTTATGCTGACACCGCTTCCATTGGCTTTGTGCGCGAGTCGGGGTTAATTGCTAATTTGGAGAAGAGTTCTATGTGTGTGGCAGGGGTTGACATTGAGGCTACTACTGTGTTGGCTAATAGTATGGGGTTTGCGCTGGGTACTTTGCCTGTTCGTTACCTGGGTGTTCCCTTACTCTCTGGTCGTATGCGTTCTTCGGATTGTGCTCCGCTCATCCAGCGTATTACTAGTCGGATTCAGTCTTAG

mRNA sequence

ATGGAGGATTATAGGCCTATTTCGTGTTGTAATGTGGTTTACAAGTGCATTTCTAAGATTTTGGCGGATAGGCTTCATGTGTGGCTTCCTTTTTTTATCGGTGGTAATCAGTCGGCCTTTGTAGTTGGGAGGAGTATTGTTGATAACATTCTGCTTTGTCAGGAGATGTCGGGGTTAATTGCTAATTTGGAGAAGAGTTCTATGTGTGTGGCAGGGGTTGACATTGAGGCTACTACTGTGTTGGCTAATAGTATGGGGTTTGCGCTGGGTACTTTGCCTGTTCGTTACCTGGGTGTTCCCTTACTCTCTGGTCGTATGCGTTCTTCGGATTGTGCTCCGCTCATCCAGCGTATTACTAGTCGGATTCAGTCTTAG

Coding sequence (CDS)

ATGGAGGATTATAGGCCTATTTCGTGTTGTAATGTGGTTTACAAGTGCATTTCTAAGATTTTGGCGGATAGGCTTCATGTGTGGCTTCCTTTTTTTATCGGTGGTAATCAGTCGGCCTTTGTAGTTGGGAGGAGTATTGTTGATAACATTCTGCTTTGTCAGGAGATGTCGGGGTTAATTGCTAATTTGGAGAAGAGTTCTATGTGTGTGGCAGGGGTTGACATTGAGGCTACTACTGTGTTGGCTAATAGTATGGGGTTTGCGCTGGGTACTTTGCCTGTTCGTTACCTGGGTGTTCCCTTACTCTCTGGTCGTATGCGTTCTTCGGATTGTGCTCCGCTCATCCAGCGTATTACTAGTCGGATTCAGTCTTAG

Protein sequence

MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQEMSGLIANLEKSSMCVAGVDIEATTVLANSMGFALGTLPVRYLGVPLLSGRMRSSDCAPLIQRITSRIQS
Homology
BLAST of PI0006148 vs. ExPASy TrEMBL
Match: A0A5A7V4C1 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00110 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 5.3e-39
Identity = 94/170 (55.29%), Postives = 108/170 (63.53%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQEM---- 60
           ME++RPISCCNV+YKCISKILADR+ VWLP FI  NQSAF+ GRSI+DNILLCQE+    
Sbjct: 197 MEEFRPISCCNVIYKCISKILADRICVWLPSFISSNQSAFMPGRSIIDNILLCQELVGGY 256

Query: 61  ------------------------------------------SGLIANLEKSSMCVAGVD 120
                                                     SGL ANL KSS+ VAGV 
Sbjct: 257 HLNFGKPRYTLKVDLQKAYDSVNWDFLFGLLIAIGTPLKFVTSGLFANLRKSSIFVAGVS 316

Query: 121 IEATTVLANSMGFALGTLPVRYLGVPLLSGRMRSSDCAPLIQRITSRIQS 125
            EA + LA SMGF LG LPVRYLG+PLL+GR+RS+DCAPLIQRITS I+S
Sbjct: 317 HEAASHLAASMGFGLGNLPVRYLGLPLLTGRLRSNDCAPLIQRITSWIRS 366

BLAST of PI0006148 vs. ExPASy TrEMBL
Match: A0A1S4E258 (uncharacterized protein LOC103496880 OS=Cucumis melo OX=3656 GN=LOC103496880 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.1e-36
Identity = 92/166 (55.42%), Postives = 106/166 (63.86%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQEMSG-- 60
           ME++RPISCCNV+YKCISKILADR+ VWLP FI  NQSAF+ GRSI+DNILLCQE+ G  
Sbjct: 197 MEEFRPISCCNVIYKCISKILADRICVWLPSFISSNQSAFMPGRSIIDNILLCQELVGGY 256

Query: 61  ------------------------------LIA----------NLEKSSMCVAGVDIEAT 120
                                         LIA             KSS+ VAGV  EA 
Sbjct: 257 HLNFGKPRYTLKVDLQKAYDSVNWDFLFGLLIAIGTPLKFERCKARKSSIFVAGVSHEAA 316

Query: 121 TVLANSMGFALGTLPVRYLGVPLLSGRMRSSDCAPLIQRITSRIQS 125
           + LA SMGF LG LPVRYLG+PLL+GR+RS+DCAPLIQRITS I+S
Sbjct: 317 SHLAASMGFGLGNLPVRYLGLPLLTGRLRSNDCAPLIQRITSWIRS 362

BLAST of PI0006148 vs. ExPASy TrEMBL
Match: A0A1S3BSI8 (uncharacterized protein LOC103493225 OS=Cucumis melo OX=3656 GN=LOC103493225 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.1e-32
Identity = 89/212 (41.98%), Postives = 106/212 (50.00%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQ------ 60
           +ED+RPISCCNV+YKCISKILADRL VWLP FI  NQSAF++GRSI++NILLCQ      
Sbjct: 399 LEDFRPISCCNVLYKCISKILADRLRVWLPSFISSNQSAFILGRSIIENILLCQELVGGY 458

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 459 HLNSGKPRCTLKVDLQKAYDSVNWDFLFGLQISISTPLKKGVRQGDPLSLFLFVMVMEVL 518

Query: 121 ----------------------EMSGLIANLEKSSMCVAGVDIEATTVLANSMGFALGTL 125
                                 E+SGL AN  KSS+ +AGV+ E  + LA  MGF  G L
Sbjct: 519 SRMLNKIPQSFQFHHRCEKRFGELSGLFANPRKSSIFIAGVNNENASRLAACMGFVRGNL 578

BLAST of PI0006148 vs. ExPASy TrEMBL
Match: A0A5A7V5J2 (Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G00210 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.8e-31
Identity = 91/201 (45.27%), Postives = 107/201 (53.23%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQEM---- 60
           ME++RPISCCNV+YKCISKILADRL VWLP FIG NQSAF+ GRSI+DNILLCQE+    
Sbjct: 753 MEEFRPISCCNVIYKCISKILADRLRVWLPSFIGSNQSAFIPGRSIIDNILLCQELVEGY 812

Query: 61  ---SG-----LIANLEKS------------------------------------------ 120
              SG     L  +L+K+                                          
Sbjct: 813 HLNSGKPRCTLKVDLQKAYDSVNWDFLKGVRQGDPLSPFLFVMVMEVLSRMLNKIPQSFK 872

Query: 121 --------------------SMCVA---GVDIEATTVLANSMGFALGTLPVRYLGVPLLS 125
                                 C A    +  EA + LA SMGF LG LPVRYLG+PLL+
Sbjct: 873 FHHRCEKVKLTHLTFADDLMIFCAADEPSLSNEAASHLAASMGFVLGNLPVRYLGLPLLT 932

BLAST of PI0006148 vs. ExPASy TrEMBL
Match: A0A5D3DSA8 (Putative non-LTR retroelement reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G002870 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.4e-30
Identity = 83/179 (46.37%), Postives = 98/179 (54.75%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQEMSGLI 60
           MED+RPISCCNV+YKCISKILADRL VWLP FI  NQSAF+ GRSI++NILLCQE+ GL+
Sbjct: 237 MEDFRPISCCNVLYKCISKILADRLRVWLPSFISSNQSAFIPGRSIIENILLCQELFGLL 296

Query: 61  ANL-------------------------------------------EKSSM--------- 120
             +                                           EK  +         
Sbjct: 297 IAIGTPLKKGVRQGDPLSPFLFVMMMEVLSCMLNKIPQSFLFHHRCEKVKLNHLTFADDL 356

Query: 121 ---CVAGVDIEATTVLANSMGFALGTLPVRYLGVPLLSGRMRSSDCAPLIQRITSRIQS 125
              C A        ++   +   LG LPVRYLG+PLLSGR+RSSDCAPLIQRITSRI+S
Sbjct: 357 MIFCAADESSLRLLLIWLLVWVVLGNLPVRYLGLPLLSGRLRSSDCAPLIQRITSRIRS 415

BLAST of PI0006148 vs. NCBI nr
Match: KAA0060725.1 (uncharacterized protein E6C27_scaffold72G00120 [Cucumis melo var. makuwa] >TYK01467.1 uncharacterized protein E5676_scaffold451G00110 [Cucumis melo var. makuwa])

HSP 1 Score: 170.2 bits (430), Expect = 1.1e-38
Identity = 94/170 (55.29%), Postives = 108/170 (63.53%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQEM---- 60
           ME++RPISCCNV+YKCISKILADR+ VWLP FI  NQSAF+ GRSI+DNILLCQE+    
Sbjct: 197 MEEFRPISCCNVIYKCISKILADRICVWLPSFISSNQSAFMPGRSIIDNILLCQELVGGY 256

Query: 61  ------------------------------------------SGLIANLEKSSMCVAGVD 120
                                                     SGL ANL KSS+ VAGV 
Sbjct: 257 HLNFGKPRYTLKVDLQKAYDSVNWDFLFGLLIAIGTPLKFVTSGLFANLRKSSIFVAGVS 316

Query: 121 IEATTVLANSMGFALGTLPVRYLGVPLLSGRMRSSDCAPLIQRITSRIQS 125
            EA + LA SMGF LG LPVRYLG+PLL+GR+RS+DCAPLIQRITS I+S
Sbjct: 317 HEAASHLAASMGFGLGNLPVRYLGLPLLTGRLRSNDCAPLIQRITSWIRS 366

BLAST of PI0006148 vs. NCBI nr
Match: XP_016902060.1 (PREDICTED: uncharacterized protein LOC103496880 [Cucumis melo])

HSP 1 Score: 162.5 bits (410), Expect = 2.3e-36
Identity = 92/166 (55.42%), Postives = 106/166 (63.86%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQEMSG-- 60
           ME++RPISCCNV+YKCISKILADR+ VWLP FI  NQSAF+ GRSI+DNILLCQE+ G  
Sbjct: 197 MEEFRPISCCNVIYKCISKILADRICVWLPSFISSNQSAFMPGRSIIDNILLCQELVGGY 256

Query: 61  ------------------------------LIA----------NLEKSSMCVAGVDIEAT 120
                                         LIA             KSS+ VAGV  EA 
Sbjct: 257 HLNFGKPRYTLKVDLQKAYDSVNWDFLFGLLIAIGTPLKFERCKARKSSIFVAGVSHEAA 316

Query: 121 TVLANSMGFALGTLPVRYLGVPLLSGRMRSSDCAPLIQRITSRIQS 125
           + LA SMGF LG LPVRYLG+PLL+GR+RS+DCAPLIQRITS I+S
Sbjct: 317 SHLAASMGFGLGNLPVRYLGLPLLTGRLRSNDCAPLIQRITSWIRS 362

BLAST of PI0006148 vs. NCBI nr
Match: XP_031745634.1 (uncharacterized protein LOC116406053 [Cucumis sativus])

HSP 1 Score: 152.9 bits (385), Expect = 1.8e-33
Identity = 79/149 (53.02%), Postives = 98/149 (65.77%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQEMSGLI 60
           +ED+ PISCC+V+YKCIS+ILADRL VWLP F+ GNQ AF+ GRSI+DNILLCQE+ G  
Sbjct: 639 LEDFSPISCCSVIYKCISRILADRLRVWLPSFVSGNQPAFIPGRSIIDNILLCQELVGAY 698

Query: 61  ANLEKSSMCVAGVDIE-------------------------ATTVLANSMGFALGTLPVR 120
                   C   VD++                          ++ LA +MGF++G LPVR
Sbjct: 699 HLHRGKPRCTMKVDLQKAYDYVNWDFLFGLLIAIGTPLRFFESSRLAANMGFSIGHLPVR 758

Query: 121 YLGVPLLSGRMRSSDCAPLIQRITSRIQS 125
           YLG+PLLS R+RSSDC PLIQRITSRI+S
Sbjct: 759 YLGLPLLSRRLRSSDCDPLIQRITSRIRS 787

BLAST of PI0006148 vs. NCBI nr
Match: XP_031740402.1 (uncharacterized protein LOC116403409 [Cucumis sativus] >XP_031740413.1 uncharacterized protein LOC116403417 [Cucumis sativus] >XP_031745405.1 uncharacterized protein LOC116405607 [Cucumis sativus] >XP_031745422.1 uncharacterized protein LOC116405638 [Cucumis sativus] >XP_031745640.1 uncharacterized protein LOC116406072 [Cucumis sativus])

HSP 1 Score: 149.8 bits (377), Expect = 1.5e-32
Identity = 85/189 (44.97%), Postives = 104/189 (55.03%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQ------ 60
           +ED+ PISCC+V+YKCIS+ILADRL VWLP F+ GNQ AF+ GRSI+DNILLCQ      
Sbjct: 302 LEDFSPISCCSVIYKCISRILADRLRVWLPSFVSGNQPAFIPGRSIIDNILLCQELVGAY 361

Query: 61  -----------------------------------------------------------E 120
                                                                      E
Sbjct: 362 HLHRGKPRCTMKVDLQKAYDYVNWDFLFGLLIAIDDLMIFCTADNHSMSFIKETIKRFGE 421

Query: 121 MSGLIANLEKSSMCVAGVDIEATTVLANSMGFALGTLPVRYLGVPLLSGRMRSSDCAPLI 125
           +SGL ANL KS + + GV+    + LA +MGF++G LPVRYLG+PLLS R+RSSDC PLI
Sbjct: 422 LSGLFANLAKSLIFLVGVNSSKASRLAANMGFSIGHLPVRYLGLPLLSRRLRSSDCDPLI 481

BLAST of PI0006148 vs. NCBI nr
Match: XP_008452126.1 (PREDICTED: uncharacterized protein LOC103493225 [Cucumis melo])

HSP 1 Score: 148.3 bits (373), Expect = 4.4e-32
Identity = 89/212 (41.98%), Postives = 106/212 (50.00%), Query Frame = 0

Query: 1   MEDYRPISCCNVVYKCISKILADRLHVWLPFFIGGNQSAFVVGRSIVDNILLCQ------ 60
           +ED+RPISCCNV+YKCISKILADRL VWLP FI  NQSAF++GRSI++NILLCQ      
Sbjct: 399 LEDFRPISCCNVLYKCISKILADRLRVWLPSFISSNQSAFILGRSIIENILLCQELVGGY 458

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 459 HLNSGKPRCTLKVDLQKAYDSVNWDFLFGLQISISTPLKKGVRQGDPLSLFLFVMVMEVL 518

Query: 121 ----------------------EMSGLIANLEKSSMCVAGVDIEATTVLANSMGFALGTL 125
                                 E+SGL AN  KSS+ +AGV+ E  + LA  MGF  G L
Sbjct: 519 SRMLNKIPQSFQFHHRCEKRFGELSGLFANPRKSSIFIAGVNNENASRLAACMGFVRGNL 578

BLAST of PI0006148 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 49.3 bits (116), Expect = 2.6e-06
Identity = 25/53 (47.17%), Postives = 35/53 (66.04%), Query Frame = 0

Query: 70  VAGVDIEATTVLANSMGFALGTLPVRYLGVPLLSGRMRSSDCAPLIQRITSRI 123
           +AGV       + +S  FA G LPVRYLG+PLL+ +M +SD  PL+++I  RI
Sbjct: 1   MAGVKDNDKADILHSFPFASGALPVRYLGLPLLTKKMTTSDYGPLVEKIRVRI 53

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7V4C15.3e-3955.29Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S4E2581.1e-3655.42uncharacterized protein LOC103496880 OS=Cucumis melo OX=3656 GN=LOC103496880 PE=... [more]
A0A1S3BSI82.1e-3241.98uncharacterized protein LOC103493225 OS=Cucumis melo OX=3656 GN=LOC103493225 PE=... [more]
A0A5A7V5J21.8e-3145.27Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. mak... [more]
A0A5D3DSA83.4e-3046.37Putative non-LTR retroelement reverse transcriptase OS=Cucumis melo var. makuwa ... [more]
Match NameE-valueIdentityDescription
KAA0060725.11.1e-3855.29uncharacterized protein E6C27_scaffold72G00120 [Cucumis melo var. makuwa] >TYK01... [more]
XP_016902060.12.3e-3655.42PREDICTED: uncharacterized protein LOC103496880 [Cucumis melo][more]
XP_031745634.11.8e-3353.02uncharacterized protein LOC116406053 [Cucumis sativus][more]
XP_031740402.11.5e-3244.97uncharacterized protein LOC116403409 [Cucumis sativus] >XP_031740413.1 uncharact... [more]
XP_008452126.14.4e-3241.98PREDICTED: uncharacterized protein LOC103493225 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G24255.12.6e-0647.17RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 2..76
e-value: 2.9E-10
score: 40.0
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 2..55
NoneNo IPR availablePANTHERPTHR33116:SF33OS01G0885550 PROTEINcoord: 2..55
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 56..124
NoneNo IPR availablePANTHERPTHR33116:SF33OS01G0885550 PROTEINcoord: 56..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0006148.1PI0006148.1mRNA