PI0006078 (gene) Melon (PI 482460) v1

Overview


Name	PI0006078
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Kunitz-type trypsin inhibitor
Location	chr05: 3127879 .. 3128847 (-)
RNA-Seq Expression	PI0006078
Synteny	PI0006078

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGACTTTGGTCATTTTCATCTCCTCCATGTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATCCTTTTTCAAACAAAACTAAATCACTCTTATTGAAGAATCAAAAGATGATGAGGTTCCATATCTCACTTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATATCCTCTACTGCCCAATTTGATGGTCTTCCGGTGCTCGACACCGAAGGCCGACCTTTGCAGCGCGATGTCGAGTATTTCGTCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAGAAGGTGATCAGTGCCCGTTTTACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTATGTATTACTAAACTCAATTGATTCAATAAGTTATGTGATATTTGTTAGAGTGCTGATATAACAGTATTAAATTTACCTACACTCACTGTTTAAGCTGATAGATTATGATATATTGAATTCTTAACAATAGGTTTTGGGGTGACCTTCGCACCTTATCGAGAGGGTGAAGATACGATCAGAGAAGGCAGAGATTTGAAGGTCGTGTTTCAAGCGTTCACAATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGACCGGAAGGAGGTTTGTAAAGCTCGGAAACGATAACACTGCCACCGGATACTTTAGGATAGACACGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAAAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCTGCTTTTCCTTTTGAGTTTGTGAGAGTTGATTCTGTAAAAGAGCTTGGTTCTATCCTGCAAGAGAAAAAATAATGTGTAAATTTGTTGTAATTGTATACACACTCAATTAAAATTAAATAAAACTCTTTGTAAGGATTTACTTTA

mRNA sequence

AGACTTTGGTCATTTTCATCTCCTCCATGTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATCCTTTTTCAAACAAAACTAAATCACTCTTATTGAAGAATCAAAAGATGATGAGGTTCCATATCTCACTTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATATCCTCTACTGCCCAATTTGATGGTCTTCCGGTGCTCGACACCGAAGGCCGACCTTTGCAGCGCGATGTCGAGTATTTCGTCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAGAAGGTGATCAGTGCCCGTTTTACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTTTTGGGGTGACCTTCGCACCTTATCGAGAGGGTGAAGATACGATCAGAGAAGGCAGAGATTTGAAGGTCGTGTTTCAAGCGTTCACAATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGACCGGAAGGAGGTTTGTAAAGCTCGGAAACGATAACACTGCCACCGGATACTTTAGGATAGACACGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAAAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCTGCTTTTCCTTTTGAGTTTGTGAGAGTTGATTCTGTAAAAGAGCTTGGTTCTATCCTGCAAGAGAAAAAATAATGTGTAAATTTGTTGTAATTGTATACACACTCAATTAAAATTAAATAAAACTCTTTGTAAGGATTTACTTTA

Coding sequence (CDS)

ATGTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATCCTTTTTCAAACAAAACTAAATCACTCTTATTGAAGAATCAAAAGATGATGAGGTTCCATATCTCACTTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATATCCTCTACTGCCCAATTTGATGGTCTTCCGGTGCTCGACACCGAAGGCCGACCTTTGCAGCGCGATGTCGAGTATTTCGTCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAGAAGGTGATCAGTGCCCGTTTTACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTTTTGGGGTGACCTTCGCACCTTATCGAGAGGGTGAAGATACGATCAGAGAAGGCAGAGATTTGAAGGTCGTGTTTCAAGCGTTCACAATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGACCGGAAGGAGGTTTGTAAAGCTCGGAAACGATAACACTGCCACCGGATACTTTAGGATAGACACGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAAAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCTGCTTTTCCTTTTGAGTTTGTGAGAGTTGATTCTGTAAAAGAGCTTGGTTCTATCCTGCAAGAGAAAAAATAA

Protein sequence

MLAIKWHYTFASQNHPFSNKTKSLLLKNQKMMRFHISLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLITREGDQCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGTQWKVGEEDPKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPFEFVRVDSVKELGSILQEKK

Homology

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BLAST of PI0006078 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 5.0e-38
Identity = 88/202 (43.56%), Postives = 119/202 (58.91%), Query Frame = 0


Query: 34  FHISLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLITR 93
           F I +    WL   +  +S AQF    V+DT G P++ D +YF++PAIT   G+LTL+TR
Sbjct: 6   FTIFILANVWL--LVVTTSIAQF----VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR 65

Query: 94  EGDQCPFYVGQVPLLSQDIGFGVTFAPY--REGEDTIREGRDLKVVFQAFTICITGTQWK 153
             + CPF VG  P   Q  GF V  +P+     ED +R GRDL+V+FQA T C   T+W+
Sbjct: 66  --NSCPFNVGLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWR 125

Query: 154 VGEEDPKTGRRFVKLGNDNTATG----YFRI-DTSDLGVYNIGWCPSDVPIKGRPRCGSA 213
           +GE D  TGRRF+  G D++  G    +FRI  T   G++NI WCP++V    +  CG+ 
Sbjct: 126 LGERDATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTV 185

Query: 214 GILIEKGVRFLALDGPAFPFEF 229
           GI+ E G   LALDG A P  F
Sbjct: 186 GIVRENGKILLALDGSALPVAF 197

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BLAST of PI0006078 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.4e-34
Identity = 79/205 (38.54%), Postives = 118/205 (57.56%), Query Frame = 0


Query: 32  MRFHI-SLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTL 91
           MR  I +L + + +C F+  ++ AQF    VLDT G P++ D EYF++P IT+  G  T+
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRSTM 62

Query: 92  ITREGDQCPFYVGQVPLLSQDIGFGVTFAPY--REGEDTIREGRDLKVVFQAFTICITGT 151
           ++R  + CP +VG + L     G  V F P+      D +R  RDL++ FQA + C+  T
Sbjct: 63  VSR-NESCPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQST 122

Query: 152 QWKVGEEDPKTGRRFVKLGNDNTATG----YFRIDTSDL-GVYNIGWCPSDVPIKGRPRC 211
           +W++GE+D K+GRR +  G D+   G    +FRI  + L G+YNI WCP++V    +  C
Sbjct: 123 EWRLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFEC 182

Query: 212 GSAGILIEKGVRFLALDGPAFPFEF 229
           G+  +L E G   LALDG   P  F
Sbjct: 183 GTVDMLNENGKILLALDGGPLPLVF 201

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BLAST of PI0006078 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.2e-33
Identity = 77/199 (38.69%), Postives = 111/199 (55.78%), Query Frame = 0


Query: 36  ISLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLITREG 95
           +++ + + +   MA +S AQF    V+DT G P++ D EYF++PAIT   G  TL+T  G
Sbjct: 6   LTIFILAHVWLLMATTSIAQF----VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG 65

Query: 96  DQCPFYVGQVPLLSQDIGFGVTFAPY--REGEDTIREGRDLKVVFQAFTICITGTQWKVG 155
             CP +VG +      +G  V F P+  +  +D +R  RDL+V F   T C   T W++G
Sbjct: 66  -PCPLHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLG 125

Query: 156 EEDPKTGRRFVKLGNDNTA---TGYFRI-DTSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 215
           E+D  +GRR +  G DN A     +FRI  T   G YNI WCP++     + +CG+ G++
Sbjct: 126 EKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVI 185

Query: 216 IEKGVRFLALDGPAFPFEF 229
            E G   LALDG A P  F
Sbjct: 186 RENGKNLLALDGDALPVVF 198

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BLAST of PI0006078 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 7.0e-16
Identity = 60/187 (32.09%), Postives = 86/187 (45.99%), Query Frame = 0


Query: 61  VLDTEGRPLQRDVEYFVKPAITDVAGNLTLITREGDQCPFYVGQVPLLSQDIGFGVTFAP 120
           V+D +G  +  +  Y+V P I    G LTL  R G  CP+ + Q      D G  V F+ 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ-ESSEVDEGIPVKFSN 89

Query: 121 YREGEDTIREGRDLKVVFQ-AFTICITGTQWKVGEEDPKTGRRFVKLG------NDNTAT 180
           +R     + E ++L +      TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 181 GYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 240
            +F+I+ S    Y   +CP      G P+C   GI I E GVR LAL    F   F +  
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKA- 209

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BLAST of PI0006078 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 9.4e-13
Identity = 52/136 (38.24%), Postives = 66/136 (48.53%), Query Frame = 0


Query: 36  ISLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLITREG 95
           +SL L   L   +AIS +      PV DTEG  L  D  Y+V PA     G LT+  R  
Sbjct: 2   VSLRLPLILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV- 61

Query: 96  DQCPFYVGQVPLLSQDIGFGVTFAPY----REGEDTIREGRDLKVVFQAFTICITGTQWK 155
             CP  V Q     +  GF V F P+       + TIR   D+++ F A TIC+  T+W 
Sbjct: 62  LPCPLLVAQ-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWH 121

Query: 156 VGEEDPKTGRRFVKLG 168
           VG+E P TG R V  G
Sbjct: 122 VGDE-PLTGARRVVTG 134

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BLAST of PI0006078 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 9.2e-112
Identity = 196/216 (90.74%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 31  MMRFHISL-ALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLT 90
           MMRFH SL ALFSWLCFFMAI STAQFDG PVLDTEGRPLQRDVEYF+KPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 91  LITREGDQCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGTQ 150
           LITR+GDQCPFYVGQVPLLSQ+IGFGVT  PY EG+DTIREGRDLK VFQAFTICITGTQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 151 WKVGEEDPKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 210
           WKVGEEDPK+GRRFVKLGNDNTATGYFRID S+LGVYNIGWCPSDVP KGRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 211 IEKGVRFLALDGPAFPFEFVRVDSVKELGSILQEKK 246
           IEKGVRFLALDGPAFPFEFVRVD+ +ELGSIL+EKK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

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BLAST of PI0006078 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 9.5e-109
Identity = 195/217 (89.86%), Postives = 201/217 (92.63%), Query Frame = 0


Query: 31  MMRFHISL-ALFSWLCFFMAISSTA-QFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNL 90
           MMRFH SL A+FS LCFFMAI STA QFDG PVLDTEGRPLQRD EYF+KPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 91  TLITREGDQCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGT 150
           TLITR+GDQCPFYVGQVPL SQ+ GFGV+ APYREGEDTIREGRDLK VFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 151 QWKVGEEDPKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGI 210
           QWKVGE DPKTGRRFVKLG DNTATGYFRID SDLGVYNIGWCPSDVPIKGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 211 LIEKGVRFLALDGPAFPFEFVRVDSVKELGSILQEKK 246
           LIEKGVRFLALDGPAFPFEFVRVD V+ELGSILQEKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEKK 217

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BLAST of PI0006078 vs. ExPASy TrEMBL
Match: A0A1S3C0M7 (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.4e-107
Identity = 193/216 (89.35%), Postives = 200/216 (92.59%), Query Frame = 0


Query: 31  MMRFHISL-ALFSWLCFFMAISSTA-QFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNL 90
           MMRFH SL ++FS LCFFMAI STA QFDG PVLDTEGRPLQRD EYF+KPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 91  TLITREGDQCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGT 150
           TLITR+GDQCPFYVGQVPLLSQ+ GF V+ APYREGEDTIREGRDLK VFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 151 QWKVGEEDPKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGI 210
           QWKVGE DPKTGRRFVKLG DNTATGYFRID SDLGVYNIGWCPSDVPIKGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 211 LIEKGVRFLALDGPAFPFEFVRVDSVKELGSILQEK 245
           LIEKGVRFLALDGPAFPFEFVRVD V+ELGSILQEK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of PI0006078 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 6.7e-86
Identity = 152/194 (78.35%), Postives = 169/194 (87.11%), Query Frame = 0


Query: 37  SLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLITREGD 96
           SL  FS+LC  MA+SS AQ    PVLDTEGRPLQ +VEYF+KPA+TDVAGNLTL+TR+GD
Sbjct: 6   SLVAFSYLCLVMAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGD 65

Query: 97  QCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGTQWKVGEED 156
           QCPF+VGQVPL SQ+IGFGVTF PY  GEDTIREGRDLKVVFQAF+ICIT T W+VGE D
Sbjct: 66  QCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETD 125

Query: 157 PKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRF 216
           P TGRRFV+ G+D+TATGYFRID  + GVYNIGWCPSDVPI+GRPRCG AGILIEKG+R 
Sbjct: 126 PTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRL 185

Query: 217 LALDGPAFPFEFVR 231
           LALDG AFPFEFVR
Sbjct: 186 LALDGSAFPFEFVR 197

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BLAST of PI0006078 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 4.0e-83
Identity = 149/194 (76.80%), Postives = 165/194 (85.05%), Query Frame = 0


Query: 37  SLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLITREGD 96
           SL  FS+LC  MA SS AQ    PVLD EGRPL+ +VEYF+KPAITDVAGNLTL+TR+GD
Sbjct: 6   SLVAFSYLCLVMAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGD 65

Query: 97  QCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGTQWKVGEED 156
           QCPF+VGQVPL SQ+IGFGVTF PY   ED IREGRDLKVVFQAF+ICIT T W+VGE D
Sbjct: 66  QCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEAD 125

Query: 157 PKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRF 216
           P TGRRFV+ G+D+TATGYFRID  + GVYNIGWCPSDVPI+GRPRCG AGILIEKG+R 
Sbjct: 126 PTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRL 185

Query: 217 LALDGPAFPFEFVR 231
           LALDG AFPFEFVR
Sbjct: 186 LALDGSAFPFEFVR 197

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BLAST of PI0006078 vs. NCBI nr
Match: XP_004139193.1 (endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus])

HSP 1 Score: 412.9 bits (1060), Expect = 1.9e-111
Identity = 196/216 (90.74%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 31  MMRFHISL-ALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLT 90
           MMRFH SL ALFSWLCFFMAI STAQFDG PVLDTEGRPLQRDVEYF+KPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 91  LITREGDQCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGTQ 150
           LITR+GDQCPFYVGQVPLLSQ+IGFGVT  PY EG+DTIREGRDLK VFQAFTICITGTQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 151 WKVGEEDPKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 210
           WKVGEEDPK+GRRFVKLGNDNTATGYFRID S+LGVYNIGWCPSDVP KGRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 211 IEKGVRFLALDGPAFPFEFVRVDSVKELGSILQEKK 246
           IEKGVRFLALDGPAFPFEFVRVD+ +ELGSIL+EKK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of PI0006078 vs. NCBI nr
Match: KAA0063688.1 (endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >TYK18339.1 endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 402.9 bits (1034), Expect = 2.0e-108
Identity = 195/217 (89.86%), Postives = 201/217 (92.63%), Query Frame = 0


Query: 31  MMRFHISL-ALFSWLCFFMAISSTA-QFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNL 90
           MMRFH SL A+FS LCFFMAI STA QFDG PVLDTEGRPLQRD EYF+KPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 91  TLITREGDQCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGT 150
           TLITR+GDQCPFYVGQVPL SQ+ GFGV+ APYREGEDTIREGRDLK VFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 151 QWKVGEEDPKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGI 210
           QWKVGE DPKTGRRFVKLG DNTATGYFRID SDLGVYNIGWCPSDVPIKGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 211 LIEKGVRFLALDGPAFPFEFVRVDSVKELGSILQEKK 246
           LIEKGVRFLALDGPAFPFEFVRVD V+ELGSILQEKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEKK 217

[Close]

BLAST of PI0006078 vs. NCBI nr
Match: XP_008455014.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo])

HSP 1 Score: 399.1 bits (1024), Expect = 2.8e-107
Identity = 193/216 (89.35%), Postives = 200/216 (92.59%), Query Frame = 0


Query: 31  MMRFHISL-ALFSWLCFFMAISSTA-QFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNL 90
           MMRFH SL ++FS LCFFMAI STA QFDG PVLDTEGRPLQRD EYF+KPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 91  TLITREGDQCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGT 150
           TLITR+GDQCPFYVGQVPLLSQ+ GF V+ APYREGEDTIREGRDLK VFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 151 QWKVGEEDPKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGI 210
           QWKVGE DPKTGRRFVKLG DNTATGYFRID SDLGVYNIGWCPSDVPIKGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 211 LIEKGVRFLALDGPAFPFEFVRVDSVKELGSILQEK 245
           LIEKGVRFLALDGPAFPFEFVRVD V+ELGSILQEK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of PI0006078 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 392.1 bits (1006), Expect = 3.5e-105
Identity = 189/216 (87.50%), Postives = 198/216 (91.67%), Query Frame = 0


Query: 31  MMRFHISLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTL 90
           MM FH SLA+FS LCFFMAISS AQFDG PVLDTEGRPLQRDVEYF+KPAITDVAGNLTL
Sbjct: 1   MMMFHKSLAIFSSLCFFMAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 60

Query: 91  ITREGDQCPFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGTQW 150
           ITR+GDQCPFYVGQVPLLSQDIGFGVTF PY  GEDTIREGRDLKVVFQAFTICIT TQW
Sbjct: 61  ITRKGDQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQW 120

Query: 151 KVGEEDPKTGRRFVKLGNDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGILI 210
           KVGE DP TGRRFVKLGND+TATGYFRID SDLGVYNIGWCPSDVPI+GRPRCGSAGILI
Sbjct: 121 KVGEADPITGRRFVKLGNDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILI 180

Query: 211 EKGVRFLALDGPAFPFEFVRVD-SVKELGSILQEKK 246
           EKG+R LALDG AFPFEFVRV+ + K+L SI QEKK
Sbjct: 181 EKGLRLLALDGSAFPFEFVRVEIAGKKLDSIFQEKK 216

[Close]

BLAST of PI0006078 vs. NCBI nr
Match: KAE8651587.1 (hypothetical protein Csa_023465 [Cucumis sativus])

HSP 1 Score: 386.0 bits (990), Expect = 2.5e-103
Identity = 180/198 (90.91%), Postives = 189/198 (95.45%), Query Frame = 0


Query: 48  MAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLITREGDQCPFYVGQVPL 107
           MAI STAQFDG PVLDTEGRPLQRDVEYF+KPAITDVAGNLTLITR+GDQCPFYVGQVPL
Sbjct: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60

Query: 108 LSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFTICITGTQWKVGEEDPKTGRRFVKLG 167
           LSQ+IGFGVT  PY EG+DTIREGRDLK VFQAFTICITGTQWKVGEEDPK+GRRFVKLG
Sbjct: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120

Query: 168 NDNTATGYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPFE 227
           NDNTATGYFRID S+LGVYNIGWCPSDVP KGRPRCGSAGILIEKGVRFLALDGPAFPFE
Sbjct: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180

Query: 228 FVRVDSVKELGSILQEKK 246
           FVRVD+ +ELGSIL+EKK
Sbjct: 181 FVRVDTGEELGSILKEKK 198

[Close]

BLAST of PI0006078 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 85.9 bits (211), Expect = 5.0e-17
Identity = 60/187 (32.09%), Postives = 86/187 (45.99%), Query Frame = 0


Query: 61  VLDTEGRPLQRDVEYFVKPAITDVAGNLTLITREGDQCPFYVGQVPLLSQDIGFGVTFAP 120
           V+D +G  +  +  Y+V P I    G LTL  R G  CP+ + Q      D G  V F+ 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ-ESSEVDEGIPVKFSN 89

Query: 121 YREGEDTIREGRDLKVVFQ-AFTICITGTQWKVGEEDPKTGRRFVKLG------NDNTAT 180
           +R     + E ++L +      TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 181 GYFRIDTSDLGVYNIGWCPSDVPIKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 240
            +F+I+ S    Y   +CP      G P+C   GI I E GVR LAL    F   F +  
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKA- 209

[Close]

BLAST of PI0006078 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 50.1 bits (118), Expect = 3.0e-06
Identity = 50/200 (25.00%), Postives = 80/200 (40.00%), Query Frame = 0


Query: 29  QKMMRFHISLALFSWLCFFMAISSTAQFDGLPVLDTEGRPLQRDVEYFVKPAITDVAGNL 88
           +K+    I+L + S    F A S+        VLD  G P+Q +V+Y++ PA     G L
Sbjct: 2   EKLTLSFITLTVLS--AIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGL 61

Query: 89  TLITRE---GDQC---PFYVGQVPLLSQDIGFGVTFAPYREGEDTIREGRDLKVVFQAFT 148
               R     D C          P +S   G  VTF+P       ++    L + F +  
Sbjct: 62  IPSNRNLSTQDLCLNLDIVQSSSPFVS---GLPVTFSPLNTKVKHVQLSASLNLEFDSTV 121

Query: 149 -ICITGTQWKVGEEDPKTGRRFVKLGND-NTATGYFRIDTSDLGVYNIGWCPSDVPIKGR 208
            +C     W++ +   +  + FV +G        +F+I   D   Y + +C    PI   
Sbjct: 122 WLCPDSKVWRI-DHSVQLRKSFVSIGGQKGKGNSWFQIQ-EDGDAYKLMYC----PISSI 181

Query: 209 PRCGSAGILI-EKGVRFLAL 220
             C +  + I + GVR L L
Sbjct: 182 VACINVSLEIDDHGVRRLVL 190

The following BLAST results are available for this feature:

BLAST of PI0006078 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	5.0e-38	43.56	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	4.4e-34	38.54	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	2.2e-33	38.69	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
Q8RXD5	7.0e-16	32.09	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P29421	9.4e-13	38.24	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of PI0006078 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGB4	9.2e-112	90.74	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A5A7V7Y2	9.5e-109	89.86	Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C0M7	1.4e-107	89.35	endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1FD70	6.7e-86	78.35	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLP6	4.0e-83	76.80	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]

BLAST of PI0006078 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139193.1	1.9e-111	90.74	endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus]	[more]
KAA0063688.1	2.0e-108	89.86	endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >T...	[more]
XP_008455014.1	2.8e-107	89.35	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo]	[more]
XP_038888505.1	3.5e-105	87.50	kunitz type trypsin inhibitor 111-like [Benincasa hispida]	[more]
KAE8651587.1	2.5e-103	90.91	hypothetical protein Csa_023465 [Cucumis sativus]	[more]

BLAST of PI0006078 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73260.1	5.0e-17	32.09	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	3.0e-06	25.00	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 60..232 e-value: 3.4E-27 score: 106.4
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 61..229 e-value: 9.4E-37 score: 126.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 37..231
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 61..77
None	No IPR available	GENE3D	2.80.10.50		coord: 59..233 e-value: 2.3E-38 score: 133.6
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 37..231
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 60..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0006078.1	PI0006078.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity