PI0006000 (gene) Melon (PI 482460) v1

Overview


Name	PI0006000
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4
Location	chr02: 23904125 .. 23907257 (+)
RNA-Seq Expression	PI0006000
Synteny	PI0006000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCAGACATTCCAAGAATAATGGATAAAATCAATTTCATCGACTTTCCCAAAAAAGAAAAAAAAAAAAAAACCAATTATTTATTATTATTATTTAAAATAAAATAAAAAGAAAAAGAAAAAGAAAAAACTCAATCGGGAAATGCCAAAGCTGGCCCACGCTGCCGAACCGATGCAATAGCTGTCCTCTTCTTCTTCTTCAATACGGAAATGATTCACATCTTTCTCCGACGAACAACCACAGCCAGAAAATTAACCCACCGCCGGCAATGGAAACTTCTAACTTTATGACCAACAAATGGATTGCAACCGCCCTCGGAATTTGGATTCAATGCATCTGTGGCGCTTCCTACACCTTTAGCATCTATTCTTCCGCTCTCAAATCCACCCAGTCCTACGACCAATCCACCCTCGACACCGTTTCCGTTTTCAAAGACATCGGCGCCAACGCCGGCATAATTTCCGGTTTTCTTTACTCCGCCGTCACTCCCCTTAACCCCCGTCGTGCCTTCGCCGGACCATGGATGGTTCACGCTGCGGGTGCGATTCAGTGGTTCCTTGGATATATTTTCATCTGGGCCGCTGTTTCTGGAGTGATCGATCGACCACCGGTGCCGGCGATGTGCTTCTTTATGTTTCTGGCTGCACATGCGCAAACGTTTTTTAATACGGCGAATGTGGTGACTGGTGTGCATAATTTTGCGAATTATAGTGGGACGATTGTGGGCATCATGAAGGTACCTACAAAACCCTTTATTGTTATTATGCAGATTGTTGGGGCTATGGACTTTTCTGAACCCTGATTGTATTTTTCTCTGTGGGAGATTTGGTTATGGTGAGTACTATTTAGATCTATGTCCCTTGAGTGTTTAAGAAATGGAGATGGACTTTGGTTAGTTGGATTCATTCTTTACAATAAATTTTATCTTGAAGCATAGAAATTGAAGAAGATGGTGGAACTACCAAACTAATTTGTCTTTCCTTATTGCTCTGTTCATCTCTGCCATATTCTAATTTTGATCAAGAACGTGACTAGATCAAAACCTCAAGTAATTGTACCACGGTGATCCTGGAAATAAGTGATCAAACGTGTCATAGTCAGTTAAGTTGAACCTATAAACATACTTGTTGTTAAAAAACAAATAGTTATCAAACAGGTCTAGTTTACAAGGAAGTTGTTAGTATTAGTTTTGTTTTTGTACTAATTTCTTCTAAGCCCAGAAATTGATGGTGTTAAAGCTATATAGAAGAAGAAGATTAAATATGTGCTGAACTATTAACTTTATAGGAGTCTAACAGTAACTTTCTACATCTGATTCTCTTTTATTCTGTGGAAAAGTGGGAATCAGTATCTGAATGGTTTCAAGGTTCTTATTCGGCAAAGTGGGGATCACTACTGCTTTCCTTTGGTGGAAAACTAAGTACTTTTTTTCTCACTTTAGCTTTTCATATTTATTCTCCAATTGGAGGCTTTTATTGTTGTAACTTTTTGGTTTTGGGTTTATTCTGTTATTTCTTTCATCAATGGAATGTTTCTATTTTAAAAAAAAGGTTTTATTTGACTAAGCTGTAGTTGCCTTTGGTGAATCTGAGAGCAGATTCCAAAAATTTTCCCCAGACTTTCTGTCTTGAATGTGCTATTTGTGAGTTCATATCATGTGTCTCCTTTTGCTTGGATAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTATATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCGGTTTTGCCCACAGTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCAAGTAACGAGATGAAACATTTGAACTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTCATGGTTGTCATCATCTTGAACAACACTTTCTCCCTTTCATCATGGACTCGTTTCTTTACATTCTCCATACTCTTGATTCTTCTTGCTGCTCCGCTTGTAATCGCCATTAATGCTCAAAAGGAGGACTTTAGAGGGTCATCTCCATCATTAATCACCGAAAAAAGTCATGTCGTTAACAAACCTGAGTCAATAGATGCTGAGGATTCTGTTGAATATCATGAGTTGCCCAGGGAAGAGAACCAGATAATGGTTGTTTCCAACTCTAGAGCCCCTCAAACCATGAATGTCTTGGAAGCTATACGTACTATAAATTTCTGGTTGTTGTTTCTAGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGAGCCAACTTGGTCAGTCGCTTGGTTACACAGCAACAGAAACGAAGACATTTGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACACCTCAGATTTTCTTCTCCACACATACGGTTGGGCAAGGCCATTGTTAATGGCAATTACTCTACTTATAATGAGCGGCGGCCACATCGTGATCGCATCCGGATTCTCTGGGAATCTGTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAATGGTCTTTGATGCCTACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATAGCCAGCCCTTTGGGATCTTACATTTTTTCTGTTCGAGTTATTGGTTACATTTACGACCGAGAAGCTGCCGGAGAACATGGCGCCTGCTCTGGCACACACTGTTTTGTGGTATCATTTCTTGTTATGGCCATCGTGGCTTTCTTGGGATTTCTTGTTGCTGCTGCACTTTTCTTTCGAACCCGGAGATTTTATCGACTGGCTTCGCAAAGAAGAGTGGAATGTCTTAGTTAAAGGTGTAAGAGAGAATTAGGACAGGTATGATGATTGCTTTTATAAAATCATGGAAGGATTTTGATGAGTGTAAAGAAATTTGTTGTCTTGCTTTGTTGAAGTTTTATACTTTTAAACACCATATCAGAGTGAGATGGAGTTGTATTTGTAAACTTTGTACTGTATCATAGGCTGATCGAGAGACCCTATCATTTTTAGCACAAATGTTTGTTAAAACAGAATTGTTAGATAATGTGTAATATGTTGGTCCTTATGTTATAATATAAACGTAAAATTAGCAGAAAGTAAAAA

mRNA sequence

GTCAGACATTCCAAGAATAATGGATAAAATCAATTTCATCGACTTTCCCAAAAAAGAAAAAAAAAAAAAAACCAATTATTTATTATTATTATTTAAAATAAAATAAAAAGAAAAAGAAAAAGAAAAAACTCAATCGGGAAATGCCAAAGCTGGCCCACGCTGCCGAACCGATGCAATAGCTGTCCTCTTCTTCTTCTTCAATACGGAAATGATTCACATCTTTCTCCGACGAACAACCACAGCCAGAAAATTAACCCACCGCCGGCAATGGAAACTTCTAACTTTATGACCAACAAATGGATTGCAACCGCCCTCGGAATTTGGATTCAATGCATCTGTGGCGCTTCCTACACCTTTAGCATCTATTCTTCCGCTCTCAAATCCACCCAGTCCTACGACCAATCCACCCTCGACACCGTTTCCGTTTTCAAAGACATCGGCGCCAACGCCGGCATAATTTCCGGTTTTCTTTACTCCGCCGTCACTCCCCTTAACCCCCGTCGTGCCTTCGCCGGACCATGGATGGTTCACGCTGCGGGTGCGATTCAGTGGTTCCTTGGATATATTTTCATCTGGGCCGCTGTTTCTGGAGTGATCGATCGACCACCGGTGCCGGCGATGTGCTTCTTTATGTTTCTGGCTGCACATGCGCAAACGTTTTTTAATACGGCGAATGTGGTGACTGGTGTGCATAATTTTGCGAATTATAGTGGGACGATTGTGGGCATCATGAAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTATATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCGGTTTTGCCCACAGTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCAAGTAACGAGATGAAACATTTGAACTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTCATGGTTGTCATCATCTTGAACAACACTTTCTCCCTTTCATCATGGACTCGTTTCTTTACATTCTCCATACTCTTGATTCTTCTTGCTGCTCCGCTTGTAATCGCCATTAATGCTCAAAAGGAGGACTTTAGAGGGTCATCTCCATCATTAATCACCGAAAAAAGTCATGTCGTTAACAAACCTGAGTCAATAGATGCTGAGGATTCTGTTGAATATCATGAGTTGCCCAGGGAAGAGAACCAGATAATGGTTGTTTCCAACTCTAGAGCCCCTCAAACCATGAATGTCTTGGAAGCTATACGTACTATAAATTTCTGGTTGTTGTTTCTAGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGAGCCAACTTGGTCAGTCGCTTGGTTACACAGCAACAGAAACGAAGACATTTGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACACCTCAGATTTTCTTCTCCACACATACGGTTGGGCAAGGCCATTGTTAATGGCAATTACTCTACTTATAATGAGCGGCGGCCACATCGTGATCGCATCCGGATTCTCTGGGAATCTGTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAATGGTCTTTGATGCCTACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATAGCCAGCCCTTTGGGATCTTACATTTTTTCTGTTCGAGTTATTGGTTACATTTACGACCGAGAAGCTGCCGGAGAACATGGCGCCTGCTCTGGCACACACTGTTTTGTGGTATCATTTCTTGTTATGGCCATCGTGGCTTTCTTGGGATTTCTTGTTGCTGCTGCACTTTTCTTTCGAACCCGGAGATTTTATCGACTGGCTTCGCAAAGAAGAGTGGAATGTCTTAGTTAAAGGTGTAAGAGAGAATTAGGACAGGTATGATGATTGCTTTTATAAAATCATGGAAGGATTTTGATGAGTGTAAAGAAATTTGTTGTCTTGCTTTGTTGAAGTTTTATACTTTTAAACACCATATCAGAGTGAGATGGAGTTGTATTTGTAAACTTTGTACTGTATCATAGGCTGATCGAGAGACCCTATCATTTTTAGCACAAATGTTTGTTAAAACAGAATTGTTAGATAATGTGTAATATGTTGGTCCTTATGTTATAATATAAACGTAAAATTAGCAGAAAGTAAAAA

Coding sequence (CDS)

ATGGAAACTTCTAACTTTATGACCAACAAATGGATTGCAACCGCCCTCGGAATTTGGATTCAATGCATCTGTGGCGCTTCCTACACCTTTAGCATCTATTCTTCCGCTCTCAAATCCACCCAGTCCTACGACCAATCCACCCTCGACACCGTTTCCGTTTTCAAAGACATCGGCGCCAACGCCGGCATAATTTCCGGTTTTCTTTACTCCGCCGTCACTCCCCTTAACCCCCGTCGTGCCTTCGCCGGACCATGGATGGTTCACGCTGCGGGTGCGATTCAGTGGTTCCTTGGATATATTTTCATCTGGGCCGCTGTTTCTGGAGTGATCGATCGACCACCGGTGCCGGCGATGTGCTTCTTTATGTTTCTGGCTGCACATGCGCAAACGTTTTTTAATACGGCGAATGTGGTGACTGGTGTGCATAATTTTGCGAATTATAGTGGGACGATTGTGGGCATCATGAAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTATATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCGGTTTTGCCCACAGTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCAAGTAACGAGATGAAACATTTGAACTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTCATGGTTGTCATCATCTTGAACAACACTTTCTCCCTTTCATCATGGACTCGTTTCTTTACATTCTCCATACTCTTGATTCTTCTTGCTGCTCCGCTTGTAATCGCCATTAATGCTCAAAAGGAGGACTTTAGAGGGTCATCTCCATCATTAATCACCGAAAAAAGTCATGTCGTTAACAAACCTGAGTCAATAGATGCTGAGGATTCTGTTGAATATCATGAGTTGCCCAGGGAAGAGAACCAGATAATGGTTGTTTCCAACTCTAGAGCCCCTCAAACCATGAATGTCTTGGAAGCTATACGTACTATAAATTTCTGGTTGTTGTTTCTAGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGAGCCAACTTGGTCAGTCGCTTGGTTACACAGCAACAGAAACGAAGACATTTGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACACCTCAGATTTTCTTCTCCACACATACGGTTGGGCAAGGCCATTGTTAATGGCAATTACTCTACTTATAATGAGCGGCGGCCACATCGTGATCGCATCCGGATTCTCTGGGAATCTGTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAATGGTCTTTGATGCCTACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATAGCCAGCCCTTTGGGATCTTACATTTTTTCTGTTCGAGTTATTGGTTACATTTACGACCGAGAAGCTGCCGGAGAACATGGCGCCTGCTCTGGCACACACTGTTTTGTGGTATCATTTCTTGTTATGGCCATCGTGGCTTTCTTGGGATTTCTTGTTGCTGCTGCACTTTTCTTTCGAACCCGGAGATTTTATCGACTGGCTTCGCAAAGAAGAGTGGAATGTCTTAGTTAA

Protein sequence

METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEYHELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVECLS

Homology

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BLAST of PI0006000 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 2.0e-31
Identity = 145/560 (25.89%), Postives = 249/560 (44.46%), Query Frame = 0


Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGIISGFLY 69
           KW      IWIQ   G ++ FS YSS LKS     Q  L+ ++V  D+G   G  SG + 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSG-IA 102

Query: 70  SAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQ 129
               PL+         +V  A A   F+GY   W  ++ +I   P   +     LA  + 
Sbjct: 103 LGYFPLS---------VVLFAAAAMGFVGYGVQWLVITNIITL-PYSLVFLCCLLAGLSI 162

Query: 130 TFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLMLAVLP 189
            +FNTA  +  + +F N     + +   + G+S AL    +N       + +LL+ +++P
Sbjct: 163 CWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVP 222

Query: 190 TVLSIMFMWFV----RIDKTESSNEMKH----LNSLSALAVIVAFYLMVVIILNNTFSLS 249
            V+S   ++ V     +D T   +  +H       L+ LAVI +F+L    +L+++ + S
Sbjct: 223 LVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHL----LLSSSSTSS 282

Query: 250 SWTRFFTFSILLIL-LAAPLVI--------AINAQKEDFRGSSPSLITEKSHVVNKPESI 309
           +   F    +LL+  L APL++         INA+      SS  ++     + N+  S+
Sbjct: 283 ARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARLN--HESSGYVMLNIDELKNQKTSV 342

Query: 310 DAEDSVEYHELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATIN 369
            ++   E+    +E N +      R     +    I  + FWL ++A  CG   GL   N
Sbjct: 343 SSKTGYEHMGTAKEGNTV------RLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSN 402

Query: 370 NMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIM 429
           N+ Q+ QSLG  +T   T V+++S ++F GR       DF+   +   R    AI LL  
Sbjct: 403 NLGQIAQSLGQNST---TLVTIYSSFSFFGRL-LSAAPDFMHKRFRLTRTGWFAIALLPT 462

Query: 430 SGGHIVIASGFSGN--LYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASP 489
                ++A   S    L   + L+G+  G  ++   +ITS++FG   +G   N +    P
Sbjct: 463 PIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIP 522

Query: 490 LGSYIFSVRVIGYIYDREAA-------GEHGACSGTHCFVVSFLVMAIVAFLGFLVAAAL 544
           +GS ++   +   IY+  A+        +   C G  C+  +F+    ++ LG + + +L
Sbjct: 523 IGSLLYGY-IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSL 574

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BLAST of PI0006000 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.3e-10
Identity = 60/208 (28.85%), Postives = 112/208 (53.85%), Query Frame = 0


Query: 334 WLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTF---VSLWSIWNFLGRFGAGYTS 393
           WL  L  V   G      NNM  +  ++     ++ +F   VSL++ ++ + R   G++S
Sbjct: 275 WLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSS 334

Query: 394 DFLLHTYGWARPLLMAITLLIMSGGHIVIASG----FSGNLY--VGSILVGICYGSQWSL 453
           + +      +RP+L+++  L+ +  H+++ SG    F    Y  V +I+ G  YGS ++L
Sbjct: 335 EAMESHV--SRPVLLSVIALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTL 394

Query: 454 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHG------ACSG 513
           +PTI ++++G+ ++GTI+ +  +A  +GS  + + +   +YD  AA E G       CSG
Sbjct: 395 VPTIVTKVWGIANLGTIWGSFILALAVGSLGYGL-LFAKVYD--AASEVGVGSMSQVCSG 454

Query: 514 THCFVVSFLVMAI-VAFLGFLVAAALFF 526
            HC+ ++F++    +AF     AAA+FF
Sbjct: 455 VHCYGLTFVITGTGLAF----AAAAVFF 473

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BLAST of PI0006000 vs. ExPASy Swiss-Prot
Match: Q96TW9 (Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 9.1e-08
Identity = 54/203 (26.60%), Postives = 98/203 (48.28%), Query Frame = 0


Query: 307 ENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTAT 366
           EN   +V N ++      L+ I+ I+ ++L  +++  +G     I NM  L +++   + 
Sbjct: 242 ENNDDLVPNHKS----KFLKFIKDISTYVLLFSLLLSIGPSEMYITNMGSLVKAITPNSL 301

Query: 367 ETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIASG--FS 426
            +   V++ ++++ L R   G  SDFL+  Y  +R  L+   +++     I IA+     
Sbjct: 302 ISDQ-VAIHAVFSTLSRLSLGALSDFLVTNYQISRSWLLLSIIVLGFFTQIFIATSTFVK 361

Query: 427 GNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYI 486
              Y+ S L G  YG  ++L PT+   I+G E  G+ + +  IA  +GS  F + V G +
Sbjct: 362 DQYYIISALSGFSYGGLFTLYPTVIFSIWGPEIFGSAWGSFMIAPAIGSTTFGM-VFGLV 421

Query: 487 YDREAAGEHGACSGTHCFVVSFL 508
           YD  A G     +  +C  + FL
Sbjct: 422 YD-SACGVFAESTTGNCVSLVFL 437

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BLAST of PI0006000 vs. ExPASy Swiss-Prot
Match: Q6FWD4 (Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.5e-07
Identity = 51/211 (24.17%), Postives = 99/211 (46.92%), Query Frame = 0


Query: 294 AEDSVEYHELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINN 353
           +++ VE H    E+++++  +++   Q M +++  R    ++    + C +G     I N
Sbjct: 244 SDNDVENHYDDNEQSRLLHATHA---QQMTLMKVFRDPVLYIFGATIFCALGPLEMFIAN 303

Query: 354 MSQLGQSL--GYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH---TYGWARPLLMAIT 413
           M  L   L  G+    +   +S++++ + L R G G T D+      +  W   L + + 
Sbjct: 304 MGSLTNVLAGGHEPAMSSALLSIYALTSTLTRLGTGLTVDYFNKRQLSVKWILLLFLVVG 363

Query: 414 LLIMSGGHIVIASGFSGN---------LYVGSILVGICYGSQWSLMPTITSEIFGLEHMG 473
           L+     +++  S    +          Y+G I+ GI YG  +++ PTIT  ++G +  G
Sbjct: 364 LVTQGKIYMLSMSSLDHSHMVTINRKLFYIG-IMQGIAYGGLFTIYPTITLMVWGEKMFG 423

Query: 474 TIFNTIAIASPLGSYIFSVRVIGYIYDREAA 491
           T + T+ IA  LGS + S  +   +YD E A
Sbjct: 424 TAYGTLMIAPALGSAL-SCLIYADVYDSECA 449

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BLAST of PI0006000 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 6.0e-297
Identity = 527/543 (97.05%), Postives = 530/543 (97.61%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           ME SNFM NKW AT LGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPTVLSIMFMWFVRIDKTES NEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQKEDFRGS PSLITE+SHVVNKPE IDAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPREE+QIMVVSNSRAPQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL HTYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLA QRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
           CLS
Sbjct: 541 CLS 543

[Close]

BLAST of PI0006000 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 6.0e-297
Identity = 527/543 (97.05%), Postives = 530/543 (97.61%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           ME SNFM NKW AT LGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPTVLSIMFMWFVRIDKTES NEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQKEDFRGS PSLITE+SHVVNKPE IDAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPREE+QIMVVSNSRAPQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL HTYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLA QRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
           CLS
Sbjct: 541 CLS 543

[Close]

BLAST of PI0006000 vs. ExPASy TrEMBL
Match: A0A0A0LUE8 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G008470 PE=4 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.4e-293
Identity = 521/543 (95.95%), Postives = 528/543 (97.24%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           M+ SNFM+NKWIAT LGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN
Sbjct: 1   MDPSNFMSNKWIATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAVTP NPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPFNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNEDPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPTVLS+MFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSVMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQKEDFRGSS SLI EKSHVVNKPESIDAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSSSSLIAEKSHVVNKPESIDAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPREENQIMVVSN+RAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREENQIMVVSNTRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYT TETKTFVSLWSIWNFLGRFGAGYTSDFL HTYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTETETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAA EHGACSG HCFVVSF VMAIVAFLGFLVAAALFFRTRRFY+LASQRRV 
Sbjct: 481 IGYIYDREAAREHGACSGIHCFVVSFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRVN 540

Query: 541 CLS 544
            +S
Sbjct: 541 RVS 543

[Close]

BLAST of PI0006000 vs. ExPASy TrEMBL
Match: A0A6J1IA87 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471070 PE=4 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 2.1e-273
Identity = 484/543 (89.13%), Postives = 512/543 (94.29%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           ME   FMTNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP + RR+  GPW+VHAAGAIQ FLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA++SGTIVGIMKGYLGLSGALLIQVYNT CNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGDPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           F+LMLAV PT+LSIMFMWFVRID+TES NEMKHLNSLSALAVIVAFYLMVVII NNTFSL
Sbjct: 181 FVLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQ+EDFR SSPS  TE++ V+NKP+S +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDFRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E Q M  +NSRAP+ MNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAA +HG+CSGTHCFVVSFLVMAIVAFLG LVAAALFFRTRRFYRLA QRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
            LS
Sbjct: 541 RLS 543

[Close]

BLAST of PI0006000 vs. ExPASy TrEMBL
Match: A0A6J1F604 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111441144 PE=4 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 7.4e-271
Identity = 481/543 (88.58%), Postives = 507/543 (93.37%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           ME   FMTNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP   RR+ AGPW+VHAAGAIQ FLGYIFIWAAVSG IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPTTRRRSSAGPWVVHAAGAIQCFLGYIFIWAAVSGFIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA++SGTIVGIMKGYLGLSGALLIQVYNT CNG+PSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGEPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAV PT+LSI FMWFVRID+TES NEMKHLNSLSALAVIVAFYLMV II NNTFSL
Sbjct: 181 FLLMLAVFPTLLSITFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVAIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQ+ED R SSPS  TE++ V+NKP+S +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDLRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E Q M  +NSRAP+ MNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SG SGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGLSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAA +HG+CSGTHCFVVSFLVMAIVAFLG LVAAALFFRTRRFYRLA QRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
            LS
Sbjct: 541 RLS 543

[Close]

BLAST of PI0006000 vs. NCBI nr
Match: XP_008453166.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo] >KAA0057917.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ98605.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1029.2 bits (2660), Expect = 1.2e-296
Identity = 527/543 (97.05%), Postives = 530/543 (97.61%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           ME SNFM NKW AT LGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPTVLSIMFMWFVRIDKTES NEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQKEDFRGS PSLITE+SHVVNKPE IDAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPREE+QIMVVSNSRAPQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL HTYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLA QRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
           CLS
Sbjct: 541 CLS 543

[Close]

BLAST of PI0006000 vs. NCBI nr
Match: XP_011659929.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN63641.1 hypothetical protein Csa_013553 [Cucumis sativus])

HSP 1 Score: 1017.3 bits (2629), Expect = 4.9e-293
Identity = 521/543 (95.95%), Postives = 528/543 (97.24%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           M+ SNFM+NKWIAT LGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN
Sbjct: 1   MDPSNFMSNKWIATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAVTP NPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPFNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNEDPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPTVLS+MFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSVMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQKEDFRGSS SLI EKSHVVNKPESIDAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSSSSLIAEKSHVVNKPESIDAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPREENQIMVVSN+RAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREENQIMVVSNTRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYT TETKTFVSLWSIWNFLGRFGAGYTSDFL HTYGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTETETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAA EHGACSG HCFVVSF VMAIVAFLGFLVAAALFFRTRRFY+LASQRRV 
Sbjct: 481 IGYIYDREAAREHGACSGIHCFVVSFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRVN 540

Query: 541 CLS 544
            +S
Sbjct: 541 RVS 543

[Close]

BLAST of PI0006000 vs. NCBI nr
Match: XP_038879631.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 964.1 bits (2491), Expect = 4.9e-277
Identity = 499/543 (91.90%), Postives = 516/543 (95.03%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           ME S FM NKWIATALGIWIQCICGASYTFSIYS ALKSTQ+YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEISGFMPNKWIATALGIWIQCICGASYTFSIYSPALKSTQAYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGIISGFLYSAVT   P R FAGPW+VHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTTPAP-RPFAGPWVVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNF +YSGT+VGIMKGYLGLSGAL+IQVY TTCNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFEHYSGTVVGIMKGYLGLSGALIIQVY-TTCNGDPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPT+LSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL
Sbjct: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           S+WTRFFTFSILLILLAAPL IAINAQKEDFR  SPS I+EKS  VNK ES+DAEDSVEY
Sbjct: 241 SAWTRFFTFSILLILLAAPLGIAINAQKEDFREPSPSFISEKSQ-VNKSESMDAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPREENQIM V+NSR PQ +NVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQ+GQS
Sbjct: 301 HELPREENQIMAVANSRTPQPLNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQIGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH+YGWARPLLMAITLL MSGGHI+IA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLGMSGGHIMIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREA  EHG+C+GTHCFVVSFLVMA+VAF G LVAAALFFRTRRFYRLA QRRVE
Sbjct: 481 IGYIYDREATEEHGSCNGTHCFVVSFLVMAVVAFFGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
           CLS
Sbjct: 541 CLS 540

[Close]

BLAST of PI0006000 vs. NCBI nr
Match: XP_022972528.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 951.0 bits (2457), Expect = 4.3e-273
Identity = 484/543 (89.13%), Postives = 512/543 (94.29%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           ME   FMTNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP + RR+  GPW+VHAAGAIQ FLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA++SGTIVGIMKGYLGLSGALLIQVYNT CNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGDPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           F+LMLAV PT+LSIMFMWFVRID+TES NEMKHLNSLSALAVIVAFYLMVVII NNTFSL
Sbjct: 181 FVLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQ+EDFR SSPS  TE++ V+NKP+S +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDFRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E Q M  +NSRAP+ MNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAA +HG+CSGTHCFVVSFLVMAIVAFLG LVAAALFFRTRRFYRLA QRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
            LS
Sbjct: 541 RLS 543

[Close]

BLAST of PI0006000 vs. NCBI nr
Match: XP_023531919.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 945.7 bits (2443), Expect = 1.8e-271
Identity = 482/543 (88.77%), Postives = 510/543 (93.92%), Query Frame = 0


Query: 1   METSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60
           ME   FMTNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP   RR+  GPW+VHAAGAIQ FLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPAARRRSSLGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA++SGTIVGIMKGYLGLSGALLIQVYNT CNG+PSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGEPSN 180

Query: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAV PT+LSIMFMWFVRID+TES NEMKHLNSLSALAVIVAFY+MVVII NNTFSL
Sbjct: 181 FLLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYVMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQ+ED R SSPS  TE++ V+NKP+S +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDLRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP +E Q M  +NSRAP+ MNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH+YGWARPLLMAITLLIMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRVE 540
           IGYIYDREAA +HG+CSGTHCFVVSFLVMAIVAFLG LVAAALFFRTRRFYRLA QRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CLS 544
            LS
Sbjct: 541 RLS 543

[Close]

BLAST of PI0006000 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 622.5 bits (1604), Expect = 3.3e-178
Identity = 320/543 (58.93%), Postives = 400/543 (73.66%), Query Frame = 0


Query: 7   MTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGIISG 66
           +  KW+A    IWIQC  GASYTF IYS+ LKSTQSYDQSTLDTVSVFKDIGANAG+ SG
Sbjct: 4   LRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSG 63

Query: 67  FLYSAVTPLNPR------RAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 126
            LY+  T    R          GPW+V A GAIQ F GY  IWA+V+G+I +PPVP MC 
Sbjct: 64  LLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCL 123

Query: 127 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 186
           FMFLAA +QTFFNTANVV+ V NFA+Y GT VGIMKG+LGLSGA+LIQ+Y T C GDP++
Sbjct: 124 FMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPAS 183

Query: 187 FLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 246
           F+L+LAV PTVLS++ M  VRI +T  +++ KHLN LSA+++I+A YLM++IIL NTF L
Sbjct: 184 FILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGL 243

Query: 247 SSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSP----SLITEKSHVVNKPESIDAED 306
           SSW    T   LL++LA PL+IA  AQ++    + P     LI+      +  +S + + 
Sbjct: 244 SSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYSPLISSPKATTSGNQSSEGDS 303

Query: 307 SVEYHELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQ 366
            VE                +   + +N+L+A++ ++FWLLFLAM+CGMGSGL+TINN+ Q
Sbjct: 304 KVE----------------AGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQ 363

Query: 367 LGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGH 426
           +G+SL Y++ E  + VSLWSIWNFLGRFGAGY SD LLH  GW RPLLMA TL  MS GH
Sbjct: 364 IGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGH 423

Query: 427 IVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIF 486
           ++IASGF GNLYVGS++VG+CYGSQWSLMPTITSE+FG+ HMGTIFNTI++ASP+GSYIF
Sbjct: 424 LIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIF 483

Query: 487 SVRVIGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQ 540
           SVR+IGYIYD+ A+GE   C G+HCF +SF++MA VAF GFLVA  LFFRT+  YR    
Sbjct: 484 SVRLIGYIYDKTASGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILV 530

[Close]

BLAST of PI0006000 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 578.9 bits (1491), Expect = 4.1e-165
Identity = 294/533 (55.16%), Postives = 387/533 (72.61%), Query Frame = 0


Query: 4   SNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGI 63
           +  +  KW+A    IWIQC  G SYTF IYS+ LKSTQSYDQSTLDTVSVFKDIG N G+
Sbjct: 3   TEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGV 62

Query: 64  ISGFLYSAVTPLNPRR----AFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMC 123
           +SG +Y+A T    RR       GPW+V   GAI  F GY  +WA+V+G+I RPPVP MC
Sbjct: 63  LSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMC 122

Query: 124 FFMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPS 183
            FMF+AA + TF NTANVV+ + NFA+Y GT VGIMKG++GLSGA+LIQ+Y   C GDP 
Sbjct: 123 LFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPK 182

Query: 184 NFLLMLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFS 243
            F+L+LA++P++LS++ M  VR+ KT + +E KHL+ LS L++I+A YLM+ IIL +T S
Sbjct: 183 TFILLLAIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 242

Query: 244 LSSWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSLITEKSHVVNKPESIDAEDSVE 303
           L SW    T ++LL+LL++PL++A+ A ++                + KP S      V+
Sbjct: 243 LPSWANAVTLAVLLVLLSSPLLVAVRAHRDS---------------IEKPLSSVYSPLVD 302

Query: 304 YHELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQ 363
             E       +M+  +    +++N+L+A+  ++FWLLFLAM+CGMGSG++TINN+ Q+G+
Sbjct: 303 NLEATTSGEILMLDED----KSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGE 362

Query: 364 SLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVI 423
           SL YT+ E  + ++LW+IWNF+GRFG GY SD+LLH  GW RPLLMA TL  M+ GH++I
Sbjct: 363 SLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLII 422

Query: 424 ASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVR 483
           ASGF GNLY GSI+VGICYGSQWSLMPTITSE+FG++HMGTI+NTI+IASP+GSYIFSVR
Sbjct: 423 ASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVR 482

Query: 484 VIGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYR 533
           +IGYIYDR   GE   C G HCF ++++V+A VAFLGFLV+  L FRT+  YR
Sbjct: 483 LIGYIYDRTIIGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYR 516

[Close]

BLAST of PI0006000 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 578.9 bits (1491), Expect = 4.1e-165
Identity = 302/538 (56.13%), Postives = 388/538 (72.12%), Query Frame = 0


Query: 6   FMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGIIS 65
           F   KW+A A  IWIQ   GASYTF IYSS LKS+QSYDQSTLDTVSV+KDIGAN GI+S
Sbjct: 3   FANTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILS 62

Query: 66  GFLYSAVTPLNPRRA--FAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCFFMF 125
           G  Y+AV          F+GPW+V   G +QWF+GY FIW A SGVI RPPV  MC FMF
Sbjct: 63  GLFYTAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMF 122

Query: 126 LAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLL 185
            A H Q FFNTA VVT V NF++Y GT VGIMKGYLGLSGA+L+Q+Y+  C GDP N++L
Sbjct: 123 FAGHCQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYIL 182

Query: 186 MLAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSW 245
           +LAV+P++L +  M FVR   T  + + KHLN LSA+++I+  YLMVVI++ N   +S  
Sbjct: 183 LLAVVPSLLILTLMPFVRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMP 242

Query: 246 TRFFTFSILLILLAAPLVIAINAQKEDFRG--SSPSLITEKSHVVNKPESIDAEDSVEYH 305
            +  +F+ LL+LLA+PL++A+ AQ+E+     S    +TE++ +++ P+   + D  +  
Sbjct: 243 MKICSFTFLLLLLASPLLVAVRAQREEEHRFLSLDFPVTERTTLLDSPKLNSSSDVKD-- 302

Query: 306 ELPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSL 365
                     V++N      MNVLEAI T NFWLLF+AM+CGMGSGLATINN+ Q+G+SL
Sbjct: 303 ----------VMTND-----MNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESL 362

Query: 366 GYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIAS 425
            Y+  +  + VSLWSIWNFLGRFG+GY SD  LH++GW RP+ MAITL +M+ GHIV+AS
Sbjct: 363 RYSTVQLNSLVSLWSIWNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMAS 422

Query: 426 GFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVI 485
           G  G+LY+GS+LVG+ YGSQWSLMPTITSEIFG+ HMGTIF TI+IASP+GSY FSV+VI
Sbjct: 423 GLLGSLYIGSLLVGLAYGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVI 482

Query: 486 GYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLASQRRV 540
           GY+YD+ A+ +  +C G HCF  SFL+MA +A LG LVA  L  RT++FY     +R+
Sbjct: 483 GYLYDKVASEDDHSCYGNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLVAKRI 523

[Close]

BLAST of PI0006000 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 563.9 bits (1452), Expect = 1.4e-160
Identity = 288/539 (53.43%), Postives = 387/539 (71.80%), Query Frame = 0


Query: 7   MTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAGIISG 66
           +  KW+A A  IWIQ   GA+YTF+IYSS LKS+QSYDQSTLD VSVFKDIG   GIISG
Sbjct: 4   INTKWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISG 63

Query: 67  FLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAA 126
           FLY+A+T  +  R F GPW+V   G +QWF+G+ FIWA+V G+I  PPVP MC F+FLA 
Sbjct: 64  FLYTAMT--SKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAG 123

Query: 127 HAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGD--PSNFLLM 186
           H+  FFNTANVVT   NF+ Y GT VGIM+G+LGLSGA+LIQ+Y+  C G+  P+ F+L+
Sbjct: 124 HSLPFFNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILL 183

Query: 187 LAVLPTVLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSWT 246
           LA++PT++  + M FVR+ +T + ++ KHL+ LSA+++I+A YLMVVI + N   LS   
Sbjct: 184 LAIVPTLVMFLAMPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSM 243

Query: 247 RFFTFSILLILLAAPLVIAINA--QKEDFRGSSPSLITEKSHVVNKPESIDAEDSVEYHE 306
           + F+F ++L+LLA+PL++A+ A  +K     S    + + S +++ P S           
Sbjct: 244 QIFSFILVLLLLASPLLVAVRALREKRQTLSSLDGPVLDTSALLDPPSS----------- 303

Query: 307 LPREENQIMVVSNSRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLG 366
                  I    +    +  N+LEA+ T+NFWLLFLAM+CGMGSG AT+NNM Q+G+SL 
Sbjct: 304 ------NIFPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLR 363

Query: 367 YTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLIMSGGHIVIASG 426
           Y++ +  + VSLWSIWNFLGRFGAGY SD  LH + W RP+ MAITL +M+ GHI++ASG
Sbjct: 364 YSSVQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASG 423

Query: 427 FSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIG 486
             G+LY GS+L+G+ YGSQWSLMPTITSEIFG+ HMGTI+ TI+IA P+GSYI SV+VIG
Sbjct: 424 VQGSLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIG 483

Query: 487 YIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYR-LASQRRVE 541
           Y YD+ A+ +  +C G+ CF  SF++MA VA  G LVA+ LFFRT +FY+ L ++R ++
Sbjct: 484 YFYDKVASEDDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKRNLK 523

[Close]

BLAST of PI0006000 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 3.5e-95
Identity = 203/553 (36.71%), Postives = 320/553 (57.87%), Query Frame = 0


Query: 3   TSNFMTNKWIATALGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGANAG 62
           T   +T +W      + I    GA+Y F IYS  +K T  YDQ+TL+ +S FKD+GAN G
Sbjct: 14  TIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVG 73

Query: 63  IISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCFFM 122
           +++G L + VTP         PW +   GAI  F GY  IW AV+  I +P V  MC ++
Sbjct: 74  VLAGLL-NEVTP---------PWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYI 133

Query: 123 FLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFL 182
            + A++Q+F NT ++VT V NF    G ++GI+KGY+GLSGA++ Q+Y      D    +
Sbjct: 134 CVGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELI 193

Query: 183 LMLAVLPTVLSIMFMWFVRIDKTE-SSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLS 242
           LM+  LP ++S  F+  +RI K +  +NE+K   +   +++ +A +LMVVII+N     +
Sbjct: 194 LMIGWLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFT 253

Query: 243 SWTRFFTFSILLILLAAPLVIAINAQKEDFRGSSPSL--------ITEKSHVVNKP-ESI 302
                 + +++++LL  P+++ I  +K+ ++    +L        +TEK  + +   +  
Sbjct: 254 QSEFGGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDD 313

Query: 303 DAEDSVEYHELPREENQIMVVSN-SRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATI 362
           D E+S E  E  +  +    V N         +L+A+ +++  +LFLA +CG+G  L  I
Sbjct: 314 DGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAI 373

Query: 363 NNMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHTYGWARPLLMAITLLI 422
           +N+ Q+G SLGY      TFVSL SIWN+ GR  +G  S+  L  Y + RPL++ + LL+
Sbjct: 374 DNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLL 433

Query: 423 MSGGHIVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPL 482
              GH++IA    G LYV S+++G C+G+QW L+  I SEIFGL++  T++N  ++ASP+
Sbjct: 434 SCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPI 493

Query: 483 GSYIFSVRVIGYIYDREAAGEHGA------------CSGTHCFVVSFLVMAIVAFLGFLV 533
           GSY+ +VRV GY+YD EA  ++ A            C GT CF +SF+++A V   G LV
Sbjct: 494 GSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLV 553

The following BLAST results are available for this feature:

BLAST of PI0006000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	2.0e-31	25.89	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
Q6CGU8	3.3e-10	28.85	Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28...	[more]
Q96TW9	9.1e-08	26.60	Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1	[more]
Q6FWD4	4.5e-07	24.17	Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM ...	[more]

BLAST of PI0006000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UT75	6.0e-297	97.05	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BWC2	6.0e-297	97.05	protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 ...	[more]
A0A0A0LUE8	2.4e-293	95.95	Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G00847...	[more]
A0A6J1IA87	2.1e-273	89.13	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]
A0A6J1F604	7.4e-271	88.58	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of PI0006000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008453166.1	1.2e-296	97.05	PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo] >KAA0057917.1 prote...	[more]
XP_011659929.1	4.9e-293	95.95	protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN63641.1 hypothetical pr...	[more]
XP_038879631.1	4.9e-277	91.90	protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida]	[more]
XP_022972528.1	4.3e-273	89.13	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima]	[more]
XP_023531919.1	1.8e-271	88.77	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0006000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74780.1	3.3e-178	58.93	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G18940.1	4.1e-165	55.16	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34350.1	4.1e-165	56.13	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34355.1	1.4e-160	53.43	Major facilitator superfamily protein	[more]
AT2G39210.1	3.5e-95	36.71	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 323..533 e-value: 4.2E-10 score: 41.3
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 216..529
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 335..529 e-value: 2.5E-10 score: 39.8
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 10..264 e-value: 1.3E-64 score: 218.1
None	No IPR available	PANTHER	PTHR21576:SF22	F25A4.25 PROTEIN	coord: 6..540
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 6..540
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 10..516 e-value: 1.55306E-117 score: 350.783

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0006000.1	PI0006000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity