PI0005945 (gene) Melon (PI 482460) v1

Overview
NamePI0005945
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAuxin efflux carrier component
Locationchr04: 8871432 .. 8874095 (+)
RNA-Seq ExpressionPI0005945
SyntenyPI0005945
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAGTGCTTTATTAACAAGGAATTTCTATAGAGAATACGAAAATTTTTCAATCCTTCTTCTGCTGGTCACCATGATTTCTTTGTCTGATGTTTATCATGTTGTGGCAGCCACAGTCCCTCTGTATGTAGCCATGATGTTAGCTTACATATCTGTAAAATGGTGGAAGTTCTTCACAGCTGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCACTGTTATCTTTTCAAGTCATTTCAGACAACAATCTCTACAAAATGAACCTCCAGTTGATATTGGCTGACTTTCTTCAGAAAATATTAGCTATTATTCTGTTGGGAGCAGTCACTAAAATCACCTCTCGTGGAGGTTTGAATTGGATTATCACTGGTCTGTCTCTGTCAACCATGCCAAATACTCTAATTCTAGGATTGCCAGTATTGAAGGCCATGTATGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTGCAGAGCATTCTTTGGTACAACATATTACTGTTTCTCTTTGAGTTCACTACCACCAAAGCAGCTTCTTTAGCTCCAGCATCAGAAGCCACAGGTAGGCATTGAAATTTGCTTTTCAAAAATAGTAGAGAATCAAATCACCTCAACTCAACTTTATAAGTCATTTATGTCTTTGCTTAGGACTTCCCGAAACAAAAATGGTCATATTCATACCAATGCCATGAACTTCAGTAACCATCCCAGTTTAAGTTTTCATGTATAACATTAGACTTCCCTCAACTGAAATGGCTGTATTCGTGCTATCAACCTCAATAACATTCCTTCATCCTTGTAAGGCCAAGAATGGAAACTTGAAAAATTTTCTATTGATTGCTTTTTTCCCTATTTCCTAAACTTTTATTCTCTTTCTACATCCATAGGTCACGAGTGCTATACTTAGGTAACTGTATTTGGGAGAAAGAATACTCAACATAGCTGTTCGAATGCCTTGAAGCTCTTCAGTTCTCATGTTGTTGAGCATATGTACTTGTTTATGAGTTATTTACTATAACACTAGGGTTTAGAGAAGTGCAATATATAAACTTTCTAACTTAGTGGTGCCGTTTTGTGTTCTGAAAATATTCTCTCTAATAATATTGTTCTCCTTTGCTCCGTAGACGTAACTAACATAATGTTAGTGAACCACGTATATCTGTATGTCGATCTCTATACGTTTATTGTATTGCTTAATTATTGATTTCGTAACAATAGTTTCACATAAATTCACATAACAAAAATGTGTAAACTAACTTTCTTCTATTCATTCATCCTAGTTATCGTGTTTGTCTTTTTGAAAAGCAGAAATTGAAATAACTCAAGAAGGACGAGCGAAAGAAGAGATTGAGGAGGCAAGAGATAGAAATGGAAGGAGTCTCAAAACCAAGTCCATTCTCTTAACAGTTGCTAGGAAGCTCATCACCAATCCCAATACTCATGCAACTATATTGGGACTCATTTGGGCAAGCATAAGATTCAGGTAAATTAGAGATGATAGCATTCGCTTTGAAAAAAGCTATAGATGTATTGGGATCATGGAACTAACATTCTCTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGAGGACTTGGTATGGCTATGTTCAGCTTGGGTCTTTTCATGGGCTCACGGACTAGCATTATAGCATGTGGCACAAAGATGACACTAGTGGCCATGGGAATGAAATTCTTAATGGGGCCTGCACTTATGGCAGCATGCTCACTTCCTCTAGGACTTAGGGGCAGATTGCTCAGAGTGGCAATTGTGCAGGTTTGTTGTAATTTGTATTACTAACAAGTCCACATCCAGGAATTGGCAATTCTGTTTAACGTGTTTGTCTCTCGATCTTTCTTCTTCTTCTGCATTGGCACAGGCAGCACTTCCTCAAGGAATTGTTCCATTTGTCTTTTCCAAAGAGTATAACATACACCCAGACGTTTTAAGCACAGGGTAACTATTGTAATTTTCATCTCTTATGTCAATTTTTAAACAAGATATTGATCCTCTCTAGTATTTAAAATGGGCTCAGATCATATAGTATAGGCTCAGGGACTCACCAACTTTATGTTCTTTATATAAATTATTATATAAAAAATTGAAGTAATACTAATGTTAGTGCTTAACTTAGTGGTAAAACTGTCATCTAACCCCCTCTAGACCCCATTCCTTATATTATTTTCCACATTATTTCTATTGAACTATAAATCTTCCATCAACAAAATTTCTGGATTTACTGCTCGCTTAGATGATTGAACTTTGCTTTCAAGCAGTTTAAGTAGAGCATTCTAGTTACATAGCTGAAGTACATACTGAAATGGGGTTTTCTTTTGGTTACAGGGTACTTCTGGGGTTGCTCATTGCACTGCCAGTAGCACTGGCCTATTACTACTTGCTGTCATTGTAAAGCATCAATGCAGCCAATGCCACCACAGGATAACAAGAAATTTAGGACATTGACGCTAAACATCAGTAAAAGAACACATGAATATATGAAGTTTTTGAGTTAAAAATTTTTTTGCCATTTTTTTTCCCGAAATGGTATACTGCTGATGATTTTAAGAAGCATGTAAAGTTTGTGCTAAAGATATCAATGACGGATGATGATATGCAAT

mRNA sequence

GGAAAGTGCTTTATTAACAAGGAATTTCTATAGAGAATACGAAAATTTTTCAATCCTTCTTCTGCTGGTCACCATGATTTCTTTGTCTGATGTTTATCATGTTGTGGCAGCCACAGTCCCTCTGTATGTAGCCATGATGTTAGCTTACATATCTGTAAAATGGTGGAAGTTCTTCACAGCTGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCACTGTTATCTTTTCAAGTCATTTCAGACAACAATCTCTACAAAATGAACCTCCAGTTGATATTGGCTGACTTTCTTCAGAAAATATTAGCTATTATTCTGTTGGGAGCAGTCACTAAAATCACCTCTCGTGGAGGTTTGAATTGGATTATCACTGGTCTGTCTCTGTCAACCATGCCAAATACTCTAATTCTAGGATTGCCAGTATTGAAGGCCATGTATGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTGCAGAGCATTCTTTGGTACAACATATTACTGTTTCTCTTTGAGTTCACTACCACCAAAGCAGCTTCTTTAGCTCCAGCATCAGAAGCCACAGAAATTGAAATAACTCAAGAAGGACGAGCGAAAGAAGAGATTGAGGAGGCAAGAGATAGAAATGGAAGGAGTCTCAAAACCAAGTCCATTCTCTTAACAGTTGCTAGGAAGCTCATCACCAATCCCAATACTCATGCAACTATATTGGGACTCATTTGGGCAAGCATAAGATTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGAGGACTTGGTATGGCTATGTTCAGCTTGGGTCTTTTCATGGGCTCACGGACTAGCATTATAGCATGTGGCACAAAGATGACACTAGTGGCCATGGGAATGAAATTCTTAATGGGGCCTGCACTTATGGCAGCATGCTCACTTCCTCTAGGACTTAGGGGCAGATTGCTCAGAGTGGCAATTGTGCAGGCAGCACTTCCTCAAGGAATTGTTCCATTTGTCTTTTCCAAAGAGTATAACATACACCCAGACGTTTTAAGCACAGGGGTACTTCTGGGGTTGCTCATTGCACTGCCAGTAGCACTGGCCTATTACTACTTGCTGTCATTGTAAAGCATCAATGCAGCCAATGCCACCACAGGATAACAAGAAATTTAGGACATTGACGCTAAACATCAGTAAAAGAACACATGAATATATGAAGTTTTTGAGTTAAAAATTTTTTTGCCATTTTTTTTCCCGAAATGGTATACTGCTGATGATTTTAAGAAGCATGTAAAGTTTGTGCTAAAGATATCAATGACGGATGATGATATGCAAT

Coding sequence (CDS)

ATGATTTCTTTGTCTGATGTTTATCATGTTGTGGCAGCCACAGTCCCTCTGTATGTAGCCATGATGTTAGCTTACATATCTGTAAAATGGTGGAAGTTCTTCACAGCTGAGCAATGTTCTGGAATAAACAAGTTTGTGGCAAAATTTTCAATCCCACTGTTATCTTTTCAAGTCATTTCAGACAACAATCTCTACAAAATGAACCTCCAGTTGATATTGGCTGACTTTCTTCAGAAAATATTAGCTATTATTCTGTTGGGAGCAGTCACTAAAATCACCTCTCGTGGAGGTTTGAATTGGATTATCACTGGTCTGTCTCTGTCAACCATGCCAAATACTCTAATTCTAGGATTGCCAGTATTGAAGGCCATGTATGGCAGTGAAGCAGATGTGCTTCTTGCCCAGATAGTTGTCTTGCAGAGCATTCTTTGGTACAACATATTACTGTTTCTCTTTGAGTTCACTACCACCAAAGCAGCTTCTTTAGCTCCAGCATCAGAAGCCACAGAAATTGAAATAACTCAAGAAGGACGAGCGAAAGAAGAGATTGAGGAGGCAAGAGATAGAAATGGAAGGAGTCTCAAAACCAAGTCCATTCTCTTAACAGTTGCTAGGAAGCTCATCACCAATCCCAATACTCATGCAACTATATTGGGACTCATTTGGGCAAGCATAAGATTCAGGTGGGGAGTAAAACTGCCAGAAGTTATTGACAGATCAATATCAATTCTATCAACTGGAGGACTTGGTATGGCTATGTTCAGCTTGGGTCTTTTCATGGGCTCACGGACTAGCATTATAGCATGTGGCACAAAGATGACACTAGTGGCCATGGGAATGAAATTCTTAATGGGGCCTGCACTTATGGCAGCATGCTCACTTCCTCTAGGACTTAGGGGCAGATTGCTCAGAGTGGCAATTGTGCAGGCAGCACTTCCTCAAGGAATTGTTCCATTTGTCTTTTCCAAAGAGTATAACATACACCCAGACGTTTTAAGCACAGGGGTACTTCTGGGGTTGCTCATTGCACTGCCAGTAGCACTGGCCTATTACTACTTGCTGTCATTGTAA

Protein sequence

MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVISDNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAKEEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRGRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Homology
BLAST of PI0005945 vs. ExPASy Swiss-Prot
Match: Q9LFP6 (Auxin efflux carrier component 8 OS=Arabidopsis thaliana OX=3702 GN=PIN8 PE=2 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.1e-106
Identity = 212/367 (57.77%), Postives = 276/367 (75.20%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS  D+YHVV+ATVPLYV+M L ++S +  K F+ EQC+GINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKI----TSRGG-LNWIITGLSLSTMPNTLI 120
           +NN +KM+ +LIL+D LQK L +++L  V +       RGG L W+ITGLS+S +PNTLI
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 LGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPAS------EAT 180
           LG+P+L A+YG EA  +L QIVVLQS++WY ILLFLFE    +A   + AS      +  
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARALPSSGASLEHTGNDQE 180

Query: 181 EIEITQEGRAKEEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRW 240
           E  I  E + +E+ EE      RS+ T  ILL   RKLI NPNT+AT++G+IWA++ FR 
Sbjct: 181 EANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWATLHFRL 240

Query: 241 GVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALM 300
           G  LPE+ID+SI +LS GGLGMAMFSLGLFM S++SIIACGTKM ++ M +KF++GPALM
Sbjct: 241 GWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFVLGPALM 300

Query: 301 AACSLPLGLRGRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALA 357
            A +  + L+  L +VAI+QAALPQG+VPFVF+KEYN+HP+++STGV+ G+LIALP  LA
Sbjct: 301 IASAYCIRLKSTLFKVAILQAALPQGVVPFVFAKEYNLHPEIISTGVIFGMLIALPTTLA 360

BLAST of PI0005945 vs. ExPASy Swiss-Prot
Match: Q0JJV0 (Probable auxin efflux carrier component 8 OS=Oryza sativa subsp. japonica OX=39947 GN=PIN8 PE=2 SV=2)

HSP 1 Score: 339.7 bits (870), Expect = 3.9e-92
Identity = 183/354 (51.69%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           M+S  D+Y V+ ATVPLYVAM+LAY+S+KWWK FT EQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MVSWKDIYLVLEATVPLYVAMILAYLSIKWWKLFTPEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
             + Y MN++LI +D LQK LA++   A++K       +W+ITG SLST+PNTLI+G+P+
Sbjct: 61  TTDPYDMNIKLIYSDILQKSLALLGFAAISKACCAEKFDWLITGFSLSTLPNTLIVGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180
           LK MYG +A  LL+QIVVLQS++WY +LLFLFE       +   +SE T           
Sbjct: 121 LKGMYGEQAGKLLSQIVVLQSLIWYTLLLFLFELRAANGMATTTSSETT----------- 180

Query: 181 EEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
                                                 GLIWA + FRW ++LP ++  S
Sbjct: 181 --------------------------------------GLIWALVGFRWHIRLPLIVSNS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRG 300
           I +LS GGLGMAMFSLGLF   +T IIACG K  L+A+ ++F +GPALM   S  +G+RG
Sbjct: 241 IRMLSDGGLGMAMFSLGLFTALQTKIIACGAKRMLLALAIRFFLGPALMGMSSYAIGMRG 300

Query: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLL 355
            LL++AIVQAALPQGIVPFVF+KEYN+  D+LST +++G+++A+PVALAYY+ +
Sbjct: 301 VLLKIAIVQAALPQGIVPFVFAKEYNVQADILSTAIIVGMMVAVPVALAYYFAM 305

BLAST of PI0005945 vs. ExPASy Swiss-Prot
Match: P0C0X5 (Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1B PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 6.9e-81
Identity = 201/554 (36.28%), Postives = 275/554 (49.64%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI++ D+YHV+ A VPLYVAM LAY SV+WW+ F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN + MNL+ + AD LQK++ + LL    ++++RG L+W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADV----LLAQIVVLQSILWYNILLFLFEFTTTK----------AASL---- 180
           LK MY + ADV    L+ QIVVLQ I+WY ++LFLFE+   +          AAS+    
Sbjct: 121 LKGMYAAAADVDSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIVSFR 180

Query: 181 -------------APASEATEIEITQEGRAKEEIEEARDR-------------------- 240
                          A    E E+  +GR +  + ++                       
Sbjct: 181 VDSDVVSLAGGGGGAAELQAEAEVGDDGRMRVTVRKSTSSRSEAACSHGTQSHSQSMQPR 240

Query: 241 -----------------------------------NGR---------------------- 300
                                              NG+                      
Sbjct: 241 VSNLSGVEIYSLQSSRNPTPRGSSFNHAEFFNIVGNGKQGDEEKGAAGGGGHSPQPVVGK 300

Query: 301 ------------------------------------------------------------ 357
                                                                       
Sbjct: 301 RKDLHMFVWSSSASPVSERAAAAAAGAVHVFGGGGADHGDAKGAQAYDEYSFGNKNEKDG 360

BLAST of PI0005945 vs. ExPASy Swiss-Prot
Match: Q0IQA5 (Probable auxin efflux carrier component 1d OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1D PE=2 SV=2)

HSP 1 Score: 297.7 bits (761), Expect = 1.7e-79
Identity = 199/558 (35.66%), Postives = 275/558 (49.28%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI++ D+YHV+ A VPLYVAM LAY SV+WW+ F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN + MNL+ + AD LQK++ + LL    ++++RG L+W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMY-------GSEADVLLAQIVVLQSILWYNILLFLFEFTTTK----------AASL- 180
           LK MY       G+++  L+ QIVVLQ I+WY ++LFLFE+   +          AAS+ 
Sbjct: 121 LKGMYAAAGAAAGADSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIV 180

Query: 181 ----------------APASEATEIEITQEGRAKEEIEEAR------------------- 240
                             A    E E+  +G+ +  + ++                    
Sbjct: 181 SFRVDSDVVSLAGGGGGAAELQAEAEVGDDGKMRVTVRKSTSSRSEAACSHGTQSHSQSM 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 QPRVSNLSGVEIYSLQSSRNPTPRGSSFNHAEFFNIVGNGKHGDEEKGAAGGGGHSPQPV 300

Query: 301 ------------------------------------------------------------ 357
                                                                       
Sbjct: 301 VGKRKDLHMFVWSSSASPVSERAAAAAAAGAVHVFGGGGADHGDAKGAQAYDEYSFGNKN 360

BLAST of PI0005945 vs. ExPASy Swiss-Prot
Match: Q5SMQ9 (Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1A PE=2 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.5e-75
Identity = 202/595 (33.95%), Postives = 269/595 (45.21%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+ +D YHV+ A VPLYVAM+LAY SVKWW+ FT +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ I AD LQK++ + +L A + ++ RG L W IT  SLST+PNTL++G+P+
Sbjct: 61  TNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEF-------------TTTKAASLAPASE 180
           LK MYG  +  L+ QIVVLQ I+WY ++LF+FE+             T    AS+    +
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARMLITEQFPDTAANIASIVVDPD 180

Query: 181 A-----------TEIEITQEGR-------------------------------------- 240
                       TE E+ ++GR                                      
Sbjct: 181 VVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIYSRRSMGFSSTTPRPSNLTNAEI 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 YSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADAFGVRTGATPRPSNYEDDASKPKYP 300

Query: 301 ------------------------------------------------------------ 357
                                                                       
Sbjct: 301 LPASNAAPMAGHYPAPNPAVSSAPKGAKKAATNGQAKGEDLHMFVWSSSASPVSDVFGGG 360

BLAST of PI0005945 vs. ExPASy TrEMBL
Match: A0A5D3DQ58 (Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G008260 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.2e-182
Identity = 351/356 (98.60%), Postives = 353/356 (99.16%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSLKTKSILLTVARKLI NPNTHATILGLIW+SIRFRWGVKLP VIDRS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSL LGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of PI0005945 vs. ExPASy TrEMBL
Match: A0A1S3B8S2 (Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103487029 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.2e-182
Identity = 351/356 (98.60%), Postives = 353/356 (99.16%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSLKTKSILLTVARKLI NPNTHATILGLIW+SIRFRWGVKLP VIDRS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSL LGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of PI0005945 vs. ExPASy TrEMBL
Match: A0A5A7UJS2 (Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001190 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.0e-180
Identity = 351/357 (98.32%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT-EIEITQEGRA 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT EIEITQEGRA
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATAEIEITQEGRA 180

Query: 181 KEEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEEIEEARDRNGRSLKTKSILLTVARKLI NPNTHATILGLIW+SIRFRWGVKLP VIDR
Sbjct: 181 KEEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDR 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLR 300
           SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSL LGLR
Sbjct: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLR 300

Query: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357

BLAST of PI0005945 vs. ExPASy TrEMBL
Match: A0A0A0LFB7 (Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_3G827360 PE=3 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.7e-180
Identity = 347/356 (97.47%), Postives = 351/356 (98.60%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEIT EGR K
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180

Query: 181 EEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSL+TKSILLTVARKLI NPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSL LGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           +LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of PI0005945 vs. ExPASy TrEMBL
Match: A0A6J1J3G0 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111482319 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.8e-177
Identity = 339/356 (95.22%), Postives = 352/356 (98.88%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFLQKILAI+LLGA+TKITSRGGL+WIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIVLLGAITKITSRGGLHWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180
           LKAMYG+EADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEI+ITQE RAK
Sbjct: 121 LKAMYGTEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQEERAK 180

Query: 181 EEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDR+ RSLKT++ILLTVARKLI+NPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRSRRSLKTRTILLTVARKLISNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRG 300
           I+ILSTGGLGMAMFSLGLFMGSR+SIIACGTKMTLVAMGMKFLMGPALMAACS PLGLRG
Sbjct: 241 ITILSTGGLGMAMFSLGLFMGSRSSIIACGTKMTLVAMGMKFLMGPALMAACSFPLGLRG 300

Query: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           RLL+VAIVQAALPQGIVPFVF+KEYNIHPDVLSTGVLLGLLIALPVAL YYYLLSL
Sbjct: 301 RLLKVAIVQAALPQGIVPFVFAKEYNIHPDVLSTGVLLGLLIALPVALVYYYLLSL 356

BLAST of PI0005945 vs. NCBI nr
Match: XP_008443438.1 (PREDICTED: putative auxin efflux carrier component 5 [Cucumis melo] >TYK25662.1 putative auxin efflux carrier component 5 [Cucumis melo var. makuwa])

HSP 1 Score: 647.1 bits (1668), Expect = 8.7e-182
Identity = 351/356 (98.60%), Postives = 353/356 (99.16%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180

Query: 181 EEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSLKTKSILLTVARKLI NPNTHATILGLIW+SIRFRWGVKLP VIDRS
Sbjct: 181 EEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSL LGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of PI0005945 vs. NCBI nr
Match: XP_038904643.1 (auxin efflux carrier component 8 [Benincasa hispida])

HSP 1 Score: 646.0 bits (1665), Expect = 1.9e-181
Identity = 348/356 (97.75%), Postives = 354/356 (99.44%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFLQKILAI+LLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIVLLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEI+ITQ+GRAK
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIDITQDGRAK 180

Query: 181 EEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSLKT+SILLTVARKLI NPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRNGRSLKTRSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRG 300
           I+ILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSL LGLRG
Sbjct: 241 INILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of PI0005945 vs. NCBI nr
Match: KAA0053741.1 (putative auxin efflux carrier component 5 [Cucumis melo var. makuwa])

HSP 1 Score: 642.5 bits (1656), Expect = 2.1e-180
Identity = 351/357 (98.32%), Postives = 353/357 (98.88%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           DNNLYKMNLQLILADFLQKILAIILLGA+TKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  DNNLYKMNLQLILADFLQKILAIILLGAITKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT-EIEITQEGRA 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT EIEITQEGRA
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATAEIEITQEGRA 180

Query: 181 KEEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEEIEEARDRNGRSLKTKSILLTVARKLI NPNTHATILGLIW+SIRFRWGVKLP VIDR
Sbjct: 181 KEEIEEARDRNGRSLKTKSILLTVARKLIINPNTHATILGLIWSSIRFRWGVKLPVVIDR 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLR 300
           SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSL LGLR
Sbjct: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLALGLR 300

Query: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357

BLAST of PI0005945 vs. NCBI nr
Match: XP_011652244.1 (auxin efflux carrier component 8 isoform X2 [Cucumis sativus] >KGN59594.1 hypothetical protein Csa_001720 [Cucumis sativus])

HSP 1 Score: 639.8 bits (1649), Expect = 1.4e-179
Identity = 347/356 (97.47%), Postives = 351/356 (98.60%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITQEGRAK 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEIT EGR K
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATEIEITHEGRPK 180

Query: 181 EEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDRS 240
           EEIEEARDRNGRSL+TKSILLTVARKLI NPNTHATILGLIWASIRFRWGVKLPEVIDRS
Sbjct: 181 EEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDRS 240

Query: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLRG 300
           ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSL LGLRG
Sbjct: 241 ISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLRG 300

Query: 301 RLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           +LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 KLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 356

BLAST of PI0005945 vs. NCBI nr
Match: XP_031738517.1 (auxin efflux carrier component 8 isoform X1 [Cucumis sativus])

HSP 1 Score: 635.2 bits (1637), Expect = 3.4e-178
Identity = 347/357 (97.20%), Postives = 351/357 (98.32%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS
Sbjct: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
           +NNLYKMN QLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV
Sbjct: 61  ENNLYKMNRQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT-EIEITQEGRA 180
           LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEAT EIEIT EGR 
Sbjct: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPASEATAEIEITHEGRP 180

Query: 181 KEEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRWGVKLPEVIDR 240
           KEEIEEARDRNGRSL+TKSILLTVARKLI NPNTHATILGLIWASIRFRWGVKLPEVIDR
Sbjct: 181 KEEIEEARDRNGRSLRTKSILLTVARKLIINPNTHATILGLIWASIRFRWGVKLPEVIDR 240

Query: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALMAACSLPLGLR 300
           SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFL+GPALMAACSL LGLR
Sbjct: 241 SISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLVGPALMAACSLALGLR 300

Query: 301 GRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357
           G+LLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL
Sbjct: 301 GKLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALAYYYLLSL 357

BLAST of PI0005945 vs. TAIR 10
Match: AT5G15100.1 (Auxin efflux carrier family protein )

HSP 1 Score: 387.1 bits (993), Expect = 1.5e-107
Identity = 212/367 (57.77%), Postives = 276/367 (75.20%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MIS  D+YHVV+ATVPLYV+M L ++S +  K F+ EQC+GINKFVAKFSIPLLSFQ+IS
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKI----TSRGG-LNWIITGLSLSTMPNTLI 120
           +NN +KM+ +LIL+D LQK L +++L  V +       RGG L W+ITGLS+S +PNTLI
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 LGLPVLKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTKAASLAPAS------EAT 180
           LG+P+L A+YG EA  +L QIVVLQS++WY ILLFLFE    +A   + AS      +  
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARALPSSGASLEHTGNDQE 180

Query: 181 EIEITQEGRAKEEIEEARDRNGRSLKTKSILLTVARKLITNPNTHATILGLIWASIRFRW 240
           E  I  E + +E+ EE      RS+ T  ILL   RKLI NPNT+AT++G+IWA++ FR 
Sbjct: 181 EANIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIGIIWATLHFRL 240

Query: 241 GVKLPEVIDRSISILSTGGLGMAMFSLGLFMGSRTSIIACGTKMTLVAMGMKFLMGPALM 300
           G  LPE+ID+SI +LS GGLGMAMFSLGLFM S++SIIACGTKM ++ M +KF++GPALM
Sbjct: 241 GWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITMLLKFVLGPALM 300

Query: 301 AACSLPLGLRGRLLRVAIVQAALPQGIVPFVFSKEYNIHPDVLSTGVLLGLLIALPVALA 357
            A +  + L+  L +VAI+QAALPQG+VPFVF+KEYN+HP+++STGV+ G+LIALP  LA
Sbjct: 301 IASAYCIRLKSTLFKVAILQAALPQGVVPFVFAKEYNLHPEIISTGVIFGMLIALPTTLA 360

BLAST of PI0005945 vs. TAIR 10
Match: AT2G01420.1 (Auxin efflux carrier family protein )

HSP 1 Score: 272.7 bits (696), Expect = 4.2e-73
Identity = 201/612 (32.84%), Postives = 267/612 (43.63%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A VPLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            N+ Y MN + + AD LQKI+ ++LL     +T  G L W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTK---------------------- 180
           L AMYG+ A  L+ Q+VVLQ I+WY +LLFLFE+   K                      
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLMMTPRPSNLTGAEIYSLSSTPRGS 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYNNTNSSV 300

Query: 301 -----------------------------------------------------AASLAPA 357
                                                                ++S +P 
Sbjct: 301 PAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWSSSASPV 360

BLAST of PI0005945 vs. TAIR 10
Match: AT2G01420.2 (Auxin efflux carrier family protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-72
Identity = 201/616 (32.63%), Postives = 267/616 (43.34%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A VPLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            N+ Y MN + + AD LQKI+ ++LL     +T  G L W+IT  SLST+PNTL++G+P+
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEFTTTK---------------------- 180
           L AMYG+ A  L+ Q+VVLQ I+WY +LLFLFE+   K                      
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLMMTPRPSNLTGAEIYSLSSTPRGS 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYNNTNSSV 300

Query: 301 -----------------------------------------------------AASLAPA 357
                                                                ++S +P 
Sbjct: 301 PAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWSSSASPV 360

BLAST of PI0005945 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.7e-72
Identity = 205/615 (33.33%), Postives = 265/615 (43.09%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A +PLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ I AD LQK++ + LL      T  G L W IT  SLST+PNTL++G+P+
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEF-------------------------- 180
           L AMYG  +  L+ QIVVLQ I+WY +LLFLFE+                          
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSLN 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 TTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMASSPRF 300

Query: 301 -------------------TTTKAASLAP-----------ASEATEIEI----------- 357
                              T  K  S AP           +++A E+ +           
Sbjct: 301 GYYPGGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNGSPVS 360

BLAST of PI0005945 vs. TAIR 10
Match: AT1G23080.1 (Auxin efflux carrier family protein )

HSP 1 Score: 268.9 bits (686), Expect = 6.0e-72
Identity = 207/619 (33.44%), Postives = 266/619 (42.97%), Query Frame = 0

Query: 1   MISLSDVYHVVAATVPLYVAMMLAYISVKWWKFFTAEQCSGINKFVAKFSIPLLSFQVIS 60
           MI+  D+Y V+ A +PLYVAM+LAY SV+WWK F+ +QCSGIN+FVA F++PLLSF  IS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  DNNLYKMNLQLILADFLQKILAIILLGAVTKITSRGGLNWIITGLSLSTMPNTLILGLPV 120
            NN Y MNL+ I AD LQK++ + LL      T  G L W IT  SLST+PNTL++G+P+
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKAMYGSEADVLLAQIVVLQSILWYNILLFLFEF-------------------------- 180
           L AMYG  +  L+ QIVVLQ I+WY +LLFLFE+                          
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSLN 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 TTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMASSPRF 300

Query: 301 -------------------TTTKAASLAP-----------ASEATEIEI----------- 357
                              T  K  S AP           +++A E+ +           
Sbjct: 301 GYYPGGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNGSPVS 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFP62.1e-10657.77Auxin efflux carrier component 8 OS=Arabidopsis thaliana OX=3702 GN=PIN8 PE=2 SV... [more]
Q0JJV03.9e-9251.69Probable auxin efflux carrier component 8 OS=Oryza sativa subsp. japonica OX=399... [more]
P0C0X56.9e-8136.28Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39... [more]
Q0IQA51.7e-7935.66Probable auxin efflux carrier component 1d OS=Oryza sativa subsp. japonica OX=39... [more]
Q5SMQ92.5e-7533.95Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PI... [more]
Match NameE-valueIdentityDescription
A0A5D3DQ584.2e-18298.60Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3B8S24.2e-18298.60Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103487029 PE=3 SV=1[more]
A0A5A7UJS21.0e-18098.32Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A0A0LFB76.7e-18097.47Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_3G827360 PE=3 S... [more]
A0A6J1J3G01.8e-17795.22Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111482319 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_008443438.18.7e-18298.60PREDICTED: putative auxin efflux carrier component 5 [Cucumis melo] >TYK25662.1 ... [more]
XP_038904643.11.9e-18197.75auxin efflux carrier component 8 [Benincasa hispida][more]
KAA0053741.12.1e-18098.32putative auxin efflux carrier component 5 [Cucumis melo var. makuwa][more]
XP_011652244.11.4e-17997.47auxin efflux carrier component 8 isoform X2 [Cucumis sativus] >KGN59594.1 hypoth... [more]
XP_031738517.13.4e-17897.20auxin efflux carrier component 8 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G15100.11.5e-10757.77Auxin efflux carrier family protein [more]
AT2G01420.14.2e-7332.84Auxin efflux carrier family protein [more]
AT2G01420.21.2e-7232.63Auxin efflux carrier family protein [more]
AT1G23080.32.7e-7233.33Auxin efflux carrier family protein [more]
AT1G23080.16.0e-7233.44Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..356
e-value: 8.1E-98
score: 325.6
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 9..351
e-value: 7.6E-100
score: 334.0
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..355
NoneNo IPR availablePANTHERPTHR31752:SF40AUXIN EFFLUX CARRIER COMPONENT 8coord: 1..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005945.1PI0005945.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity