PI0005873 (gene) Melon (PI 482460) v1

Overview
NamePI0005873
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionHydroxymethylglutaryl-CoA lyase
Locationchr04: 8178217 .. 8183966 (-)
RNA-Seq ExpressionPI0005873
SyntenyPI0005873
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATGGACGTATCGGGTTATGATCTTCATCTTCATCTTCATCTTGCTTATAGGTTTCTTACTGGGCTTTCCTTCCTGGGATTATGCAAGCTGCAAAGCTATCTGCTAAATTGACTCGACAAGGTTTTCCTTGGCGCCATGCATATAATCTATCTATGGAAACTCCCACCCGATTTCTTGTTTCACCTTCCGCTACGGATGTAGGCGTCAACTCTATCAGAAGGCTTGGCATAAAACGAGAATTTGATAATTGCAGGTATAGACGAAATGATTCTGATTAATGATTATAGGGAGGATTACTCGTACCCTTTTGATTTTGAAACTTTAGTTTTGTATTAAACCACTCGATTATCGTTGCAATAGCATTTGCGGAAAATGTGCTGTAGAATTCTTTCAATCCTTTAGGGATGAGTCATGGAATATGCATCGTGAAATTAGGGCATTTTTGTTTTATAAATTTATGATGAGAAATCTACTTCATCTACTTTGTGGGGGTCCTTTCAAGAGTAGAGCTAATAATTTGATTGGGTTTGGAGAGAATAGTTTGATTAATTTCAATTTGCAATCTTCGCTTTCAGTTTCTATACACGGTGAATGTAGCAATTATCCCTTTGGATTTAATAGAGATCAGGAGGTTCTTGAAAGCTCTGATGGGGAGCATGGTCCCTCGTAGGCTATACTTGGCTTGGTCTGTTGGACTTTCTTAGTAACAGCACAGAACTTGTGTAATAGAATCTAGAAGTATTATAACTTATCTAATGATGTATACATTTGAATTAACTGTGGTATCTATTTATGATTTTCTTTAAGTCTCGTATTAAGTTTGAATGTAATGCAACATTCCATTTCTTTCTCTCCAGTCAACAAGATGGAAGAGATGCAGTCCTTTCGAGAGAAACTAAGGGGGGATTGTCTCAGGTGATGTTCTAAAATGGATTGTTCTTAGTTTAAGATGTTAAAACTGGGAGTTGAGTATTCTTCATCAGGTCGAGCATTTACCGAATTGTTAGTATGGCTCCCACAATTGTAAATTGTCTATGGAAAACACAACAAAGGCGTTTCAAATGTTAAAATAATTTTTTCCCCTTGTGCATAATTGGGTTCTGTTTCCCGTCAAGATCTTATGATATGATTTGAAAAAGTCTTTTTGTCTTTTGTTTTTTTTTTTAGTTTTAGTTTTTCTGTTTGGTTTTTTTCCTCTTCTTTTTTTTACTGTAATCTTATCATAACTATTTGCAATATTGTTTTCAATTATAATGGTTATTTAATATATTCCGTGGTTTCTGTTTCTTATTAAAAAAAAAAAGAAAAAAAAGAAATAAATATATTGAATTGTTTGATGAAAGTCTTTTTCTTCCTTTGTAGTATTTATGCCGTATTCCACCATTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCTACTGCTGTTAAGGTTGATTTAATAAAGATGCTAGTCTCTTCTGGGTTGCCTGTTGTTGAAGCCACAAGTTTTGTTTCTCCAAAATGGGTACCGCAGGTAACGTAGCTTGATACTATGAAAATGTTGGTTGGTTTATTTTTCTATTCCTAAAACAGTTACTTTGTCTCATTCTTCATACTGCTGGCCTTCAACTTTTCATGTGCTCTGCCTTTGTGTCTGAGTTTCTTGTATGTTTACTTGTTAATCTCCTTTCCTCATATTTTCCTCAGTTAGCAGATGCAAACAATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTATTGACCCCAAATCTTAAAGTACGAGCTTTAACACAGAAACACAAAATTAGGTGTATTAAAAAAAACAAAAACAAAATTAGGACTAATCTGGAAGGCATTTTGTGATTTTTGGACTTTTGAGATTTTTGACTTCTTTGTCTAGGGTTTTGAAGCAGCTGTAGCAGCTGGAGCAAAGGAAGTAGCCATCTTTGCTTCAGCATCTGAATCCTTCTCGAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTTCATCGCTACCGCCAAGTTGCTGATGCGGCAAGGAAGCACTCACTTCCAGTTCGTGGGTAGGTATATGAACCAGAAAGCTGAATAATTTTAACAATCATACATCTTGCTTGATTTTGTTACTTTTAGTGTTGAGGCTATAATACTTCGATCCCTTTATGTCTACAAAATAATACTGTTGATACGCTCATAAGCCAACAGGCATATCATCTATAAAAAGTCTACCTCTATCTGGCATAAGAGCACTTTGGTTGGTTTAAATGATCCGATCCAATACCCTTCTCAAATGGTTAGCAAGAATGTTTGACATAATTTTGTAGATCAGTGATCACATTACATGTACTTGTTGTCTGAAAGACAGATTCAATTCATTAATATTGGGCTTATGGAGTTTGATTGAAGGATCATGCAATTTTCATTGAAATCAACACCTCTAGGAAAGCCCCCTCTACGTCGATTTCGTCTCAGAATTTTCAGAGCTTTTGATTGAACACTGCAAGCAAACCATCTAGATTTGAACCTTCACTAGGGCACATGTTACTACAATTGGAGGTTCCTCTCCATCAATCCAAATTGGTGTTGGTAATGCCTCTTTTTATTAGAAACCAAACTTTCATTGGGAAGAAAAACAAAAGAATAAAAGAGGCATACAAAAAAGCCAACCCTAAAAAGGAGCCAACCTGATGTAAATGGCTCCAAAAAAGTATGAATGAGAACATGTAATGGTTACTTACTAATTATAAAGTCCTTTCACACTAAAGCCCAAAGGGAGGTACAGAACCTAGCAGGTATGGATCCAACTTTCTCCTCAGATCTCTTAAACTCCTACGAGAACTCTATTGGTTCCTCTCACCCTGAAGACCTCAAAACATTTTCATCTATTTTGTAACTTTTTTAGGAACTCTTGTGTTTCAACAGCTTTTGCCTTCAATAGAGGATGTTGAAAATCTTTTTGAACGTTTGAGTATAATTAACATCAATGCCAGCCTCTTCCCTTCTTTTGATTTGATGCTCTGCTGTGAAATCAATCCTCCATTAACCTTTGCTTTTAGCTGGTTTTCTTCGTTCCAAAATATTTTCTAGCTTTCTCAGGACCTAAGGTGAAGGTGCTTTGGTTTAACGCCTGAAGTGTTCTTCTTTGGAATGTGTGGTTGGAATGTATAGGAGATGTTTCAGAGACAAGGATAGTTCAAGAGAGCAAATTTTGAAATAAGTTATTTCCTTTTTGTTGTTTTAGTTTCTAAATTCTAAAAACGTCTGTAATTATAGTCTTACATTGTTGACCTTTGTAGAGCAATTTTGGCGTTCTTTGTAGTTTCTCTTTCTTATTAAAAAAAAGAGATAATTGTAGTCGGACGTTATTACCAAAATATTTTTTTAATGTTTTAATTGCATCTGCCTCCTTTTACTATTGGAGGTGGGAGGATGTCCAGCCCTCCCATCTTATATGTGTTTCCTATAAATTAAAATGTAATTACGAACGATAATTATATGTTTATTTAGATTTAAAATCTTTTGGGGGAGTGATATGATTCTCTTTTTATATTTTGAAAATGTCTCCTATAAACATGTTAGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCGGCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCAGAGATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGTAACCTCTAGGACAATCATCTTTCAAGCATTTTTTGTACTTTGCTAAGTGAGTGAGATCAGGAGGGGCTCTCAGATCTCTATGGCTTGTATGATTATTGGCATAATTTTATCATAATTTTTAAGAGAGAATTTCCCATAGAACACTTATATTCTATTCAATTCTTATAATCATCTTATTGAAAGCATCAAGTTATGAACTTAAAAAAAGTATCATTATTTAGATATTGCATCATAAATTTTTTTGGTCAGGAATATTGCTTGGCAAAGCTTGCTTTCTTCTTCTTCTTCTTCTTCTTCTTTAACTTTAGTTTTTTGCAACTGCAGGAACTGTGATTCCAATGCTTGAATCTGTAGTTAATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTACGGGCAAGCTCTGTCCAATATTCTAGTCTCACTCCAGGTATTCTCTTCTTTTCCATCTTGGTTTTAATTGCTCGGAACCTACAAGTCCTCAAGCAATATATACCAATGAATTGGTATTTATTTGGCGAGTATCTGATAGAACCACTGTATATATTCAGAGAATCCAAGGCACAATTTAGGGACATCAAAGAGACCTTATCTCTCGTACGCTACCTTGCAGTTGCACTTAGCAAGAATCCTTGTACCCGATCCCTTTCTAGCGTCCTATATACTCCTAGGCAACCTACTCACACAACTATCTTATAGCGGCCCACAAATTGAAATTTTGTCGTCTTTTTAGACATGAAATTCAATGTTATGTTTAACGATCATTTTAACACTTTAGAAAATGCCAAGTAAAATTTTTTAAATACAACTTAATAGTTTAAGAACTAGATCTTGAACAACAAGTGTTGCTGACGGATGTGTCAAATAGTAAAGGGAAGGTACTATGTACTACCATAGTACTGAAGATTTTCCCCCTTCACTTGCTTTCTCTTGGGTTTTATTAAGAGGAAAAATATACAAAGCACCTAGTTACCAATATATATACACATATAGGGTTCAAAAAAGACAAAATGAAGACCTTGTCCTAGAATTTTTAAACTTAGAATTAGATTTTTGTGAAAGAAGCATATTTTTATAGTCATGGTTTTTTAAAAATAAGCTTATTAAACACTACTCCCACTCCACCTACGAATTACACTATTTAATTGTCACTGGTAACAAAAACGATTATGTTACACTTGCATGAAACGGATACACTAATGTTGAAAAACATTTTATCTGGTTATCAGATGGGGATCAGCACGGTGGATTCTTCGGTTTCGGGTCTTGGAGGTTGTCCATATGCTAAGGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGGGTGAAAACCAATGTAGATCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTGCAAGCACTTGGGGCGTCCTTCAGCTTCAAAAGTAACAATTGCATTGCGGAAAGTTGCCTCCAACGCCAATAAATGCACAGCCACTGCTTCCAAGTTATGAGGTACATTTTACCAAAGCTGTAAAAAATCTGCATTCTCCACTCTATCTTCTCTGATATTGTTCCCCAATACCCATAATTTGTATACCTCTTTTGAGCTTCTTCTAATTTTTAGGTGCAAAACTACTTAGTGTTTGCTGGTCTCATATTACATGAAATGAAAGAATAGATTAAGAATAAATTATGAGTATATGAAGAATCGTACTTTCATTTAGAAAAGAAGTGGGATAGAAAAAGAGAGCCACAAAAGGGAGACACGTTGAACAAAATACAAGATCAAACCTCATATGGATTCCTCGAACCCTCTAATTGAAGAGGGTATTAGCCAATCATTTTGACACAACTCATCATTCAGTTTAGGTGAAAGGAACATTTCTTGTTTATCTAATCATGGGCATGTTTGTGAAGAAAATTCTGGCTTTGACATAGTGAATGCCGAATTGACGAAGTTGATGGAAAATGGACATGTGAAGAAATAATGGAGTCGGTTTCAGATATTGTTT

mRNA sequence

GATGGACGTATCGGGTTATGATCTTCATCTTCATCTTCATCTTGCTTATAGGTTTCTTACTGGGCTTTCCTTCCTGGGATTATGCAAGCTGCAAAGCTATCTGCTAAATTGACTCGACAAGGTTTTCCTTGGCGCCATGCATATAATCTATCTATGGAAACTCCCACCCGATTTCTTGTTTCACCTTCCGCTACGGATGTAGGCGTCAACTCTATCAGAAGGCTTGGCATAAAACGAGAATTTGATAATTGCAGTCAACAAGATGGAAGAGATGCAGTCCTTTCGAGAGAAACTAAGGGGGGATTGTCTCAGTATTTATGCCGTATTCCACCATTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCTACTGCTGTTAAGGTTGATTTAATAAAGATGCTAGTCTCTTCTGGGTTGCCTGTTGTTGAAGCCACAAGTTTTGTTTCTCCAAAATGGGTACCGCAGTTAGCAGATGCAAACAATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTATTGACCCCAAATCTTAAAGGTTTTGAAGCAGCTGTAGCAGCTGGAGCAAAGGAAGTAGCCATCTTTGCTTCAGCATCTGAATCCTTCTCGAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTTCATCGCTACCGCCAAGTTGCTGATGCGGCAAGGAAGCACTCACTTCCAGTTCGTGGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCGGCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCAGAGATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGAACTGTGATTCCAATGCTTGAATCTGTAGTTAATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTACGGGCAAGCTCTGTCCAATATTCTAGTCTCACTCCAGATGGGGATCAGCACGGTGGATTCTTCGGTTTCGGGTCTTGGAGGTTGTCCATATGCTAAGGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGGGTGAAAACCAATGTAGATCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTGCAAGCACTTGGGGCGTCCTTCAGCTTCAAAAGTAACAATTGCATTGCGGAAAGTTGCCTCCAACGCCAATAAATGCACAGCCACTGCTTCCAAGTTATGAGGTACATTTTACCAAAGCTGTAAAAAATCTGCATTCTCCACTCTATCTTCTCTGATATTGTTCCCCAATACCCATAATTTGTATACCTCTTTTGAGCTTCTTCTAATTTTTAGGTGCAAAACTACTTAGTGTTTGCTGGTCTCATATTACATGAAATGAAAGAATAGATTAAGAATAAATTATGAGTATATGAAGAATCGTACTTTCATTTAGAAAAGAAGTGGGATAGAAAAAGAGAGCCACAAAAGGGAGACACGTTGAACAAAATACAAGATCAAACCTCATATGGATTCCTCGAACCCTCTAATTGAAGAGGGTATTAGCCAATCATTTTGACACAACTCATCATTCAGTTTAGGTGAAAGGAACATTTCTTGTTTATCTAATCATGGGCATGTTTGTGAAGAAAATTCTGGCTTTGACATAGTGAATGCCGAATTGACGAAGTTGATGGAAAATGGACATGTGAAGAAATAATGGAGTCGGTTTCAGATATTGTTT

Coding sequence (CDS)

ATGCAAGCTGCAAAGCTATCTGCTAAATTGACTCGACAAGGTTTTCCTTGGCGCCATGCATATAATCTATCTATGGAAACTCCCACCCGATTTCTTGTTTCACCTTCCGCTACGGATGTAGGCGTCAACTCTATCAGAAGGCTTGGCATAAAACGAGAATTTGATAATTGCAGTCAACAAGATGGAAGAGATGCAGTCCTTTCGAGAGAAACTAAGGGGGGATTGTCTCAGTATTTATGCCGTATTCCACCATTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCTACTGCTGTTAAGGTTGATTTAATAAAGATGCTAGTCTCTTCTGGGTTGCCTGTTGTTGAAGCCACAAGTTTTGTTTCTCCAAAATGGGTACCGCAGTTAGCAGATGCAAACAATGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTATTGACCCCAAATCTTAAAGGTTTTGAAGCAGCTGTAGCAGCTGGAGCAAAGGAAGTAGCCATCTTTGCTTCAGCATCTGAATCCTTCTCGAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTTCATCGCTACCGCCAAGTTGCTGATGCGGCAAGGAAGCACTCACTTCCAGTTCGTGGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCGGCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCAGAGATTTCCCTTGGCGATACAATTGGTGTTGGTACTCCAGGAACTGTGATTCCAATGCTTGAATCTGTAGTTAATGTTGTTCCAATTGAAAATCTTGCTGTTCATTTCCATGACACTTACGGGCAAGCTCTGTCCAATATTCTAGTCTCACTCCAGATGGGGATCAGCACGGTGGATTCTTCGGTTTCGGGTCTTGGAGGTTGTCCATATGCTAAGGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGGGTGAAAACCAATGTAGATCTTGAAAAGCTCATGTTGGCTGGAGATTTCATCTGCAAGCACTTGGGGCGTCCTTCAGCTTCAAAAGTAACAATTGCATTGCGGAAAGTTGCCTCCAACGCCAATAAATGCACAGCCACTGCTTCCAAGTTATGA

Protein sequence

MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQDGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILVSLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPSASKVTIALRKVASNANKCTATASKL
Homology
BLAST of PI0005873 vs. ExPASy Swiss-Prot
Match: O81027 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HMGCL PE=1 SV=2)

HSP 1 Score: 483.0 bits (1242), Expect = 3.1e-135
Identity = 255/378 (67.46%), Postives = 302/378 (79.89%), Query Frame = 0

Query: 12  RQGFPW-RHAYNLSM-ETPTRFLVSPSATDVGVNSIRRLGIKRE--FDNCSQQDGRDAVL 71
           ++ FPW RH   LS  E   R +     T     ++R     +E  +   S ++G   + 
Sbjct: 98  KESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTSHIS 157

Query: 72  SRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEAT 131
           ++ +KG        IP FVKIVEVGPRDGLQNEK+IVPT+VKV+LI+ LVSSGLPVVEAT
Sbjct: 158 NKISKG--------IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEAT 217

Query: 132 SFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESF 191
           SFVSPKWVPQLADA +VM+ +  ++GAR PVLTPNLKGF+AAV+AGAKEVAIFASASESF
Sbjct: 218 SFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESF 277

Query: 192 SKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYS 251
           S SNINC+IEESL RYR VA AA++HS+PVRGYVSCVVGCP+EG V  S VAYV KELY 
Sbjct: 278 SLSNINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYD 337

Query: 252 MGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILVSLQMGIS 311
           MGC EISLGDTIG+GTPG+V+PMLE+V+ VVP + LAVHFHDTYGQAL+NILVSLQMGIS
Sbjct: 338 MGCFEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGIS 397

Query: 312 TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPS 371
            VDSS++GLGGCPYAKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFI KHLGRP+
Sbjct: 398 IVDSSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPN 457

Query: 372 ASKVTIAL-RKVASNANK 385
            SK  +AL R++ ++A+K
Sbjct: 458 GSKAAVALNRRITADASK 467

BLAST of PI0005873 vs. ExPASy Swiss-Prot
Match: P35915 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL PE=1 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.6e-110
Identity = 199/292 (68.15%), Postives = 241/292 (82.53%), Query Frame = 0

Query: 83  PPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADAN 142
           P  VK+VEVGPRDGLQNEK +VPT VK+ LI ML  +GLPV+EATSFVSP+WVPQ+AD  
Sbjct: 3   PQRVKVVEVGPRDGLQNEKSVVPTPVKIRLIDMLSETGLPVIEATSFVSPRWVPQMADHA 62

Query: 143 NVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHR 202
            VM+GI  + G  +PVLTPNLKGF+AAVAAGAKEV+IF +ASE F+K NINCSIEESL R
Sbjct: 63  EVMQGINKLPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLER 122

Query: 203 YRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVG 262
           + +V +AAR  S+PVRGYVSCV+GCP EG + A+ VA V+K++YSMGC EISLGD IG+G
Sbjct: 123 FSEVMNAARAASIPVRGYVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIG 182

Query: 263 TPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILVSLQMGISTVDSSVSGLGGCPYA 322
           TPG++  ML +V+  VP+  LAVH HDTYGQAL+NILV+LQMG+S VD+SV+GLGGCPYA
Sbjct: 183 TPGSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDASVAGLGGCPYA 242

Query: 323 KGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPSASKVTIA 375
           +GASGNVATED+VYMLNG+G+ T VDL+KLM  G FIC  L R + SKV+ A
Sbjct: 243 QGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKVSQA 294

BLAST of PI0005873 vs. ExPASy Swiss-Prot
Match: P97519 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Hmgcl PE=2 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 3.0e-109
Identity = 199/297 (67.00%), Postives = 241/297 (81.14%), Query Frame = 0

Query: 82  IPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADA 141
           +P  VKIVEVGPRDGLQNEK IVPT VK+ LI ML  +GLPV+EATSFVSPKWVPQ+AD 
Sbjct: 29  LPKRVKIVEVGPRDGLQNEKSIVPTPVKIKLIDMLSEAGLPVIEATSFVSPKWVPQMADH 88

Query: 142 NNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLH 201
           ++V++GI+   G  +PVLTPN+KGFE AVAAGAKEV+IF +ASE F++ N+NCSIEES  
Sbjct: 89  SDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSIFGAASELFTRKNVNCSIEESFQ 148

Query: 202 RYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGV 261
           R+  V  AAR  S+ VRGYVSC +GCP EG V  + VA VAK+LYSMGC EISLGDTIGV
Sbjct: 149 RFDGVMQAARAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGV 208

Query: 262 GTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILVSLQMGISTVDSSVSGLGGCPY 321
           GTPG +  ML +V++ VP+  LAVH HDTYGQAL+N LV+LQMG+S VDSSV+GLGGCPY
Sbjct: 209 GTPGLMKDMLTAVLHEVPVAALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPY 268

Query: 322 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPSASKVTIALRKV 379
           AKGASGN+ATED+VYML G+G+ T V+L+KL+ AGDFIC+ L R ++SKV  A  K+
Sbjct: 269 AKGASGNLATEDLVYMLTGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL 325

BLAST of PI0005873 vs. ExPASy Swiss-Prot
Match: P38060 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcl PE=1 SV=2)

HSP 1 Score: 394.0 bits (1011), Expect = 1.9e-108
Identity = 197/297 (66.33%), Postives = 240/297 (80.81%), Query Frame = 0

Query: 82  IPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADA 141
           +P  VKIVEVGPRDGLQNEK IVPT VK+ LI ML  +GLPV+EATSFVSPKWVPQ+AD 
Sbjct: 29  LPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADH 88

Query: 142 NNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLH 201
           ++V++GI+   G  +PVLTPN+KGFE AVAAGAKEV++F + SE F++ N NCSIEES  
Sbjct: 89  SDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQ 148

Query: 202 RYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGV 261
           R+  V  AA+  S+ VRGYVSC +GCP EG V  + VA VAK+LYSMGC EISLGDTIGV
Sbjct: 149 RFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGV 208

Query: 262 GTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILVSLQMGISTVDSSVSGLGGCPY 321
           GTPG +  ML +V++ VP+  LAVH HDTYGQAL+N LV+LQMG+S VDSSV+GLGGCPY
Sbjct: 209 GTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPY 268

Query: 322 AKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPSASKVTIALRKV 379
           AKGASGN+ATED+VYMLNG+G+ T V+L+KL+ AGDFIC+ L R ++SKV  A  K+
Sbjct: 269 AKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL 325

BLAST of PI0005873 vs. ExPASy Swiss-Prot
Match: A8WG57 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Danio rerio OX=7955 GN=hmgcll1 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 7.3e-108
Identity = 193/298 (64.77%), Postives = 238/298 (79.87%), Query Frame = 0

Query: 79  LCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQL 138
           L ++P +VKIVEVGPRDGLQNEK IVPT VK+ LI +L  +GLPV+EATSFVS KWV Q+
Sbjct: 36  LPKLPVYVKIVEVGPRDGLQNEKEIVPTEVKIQLIDLLSQTGLPVIEATSFVSSKWVAQM 95

Query: 139 ADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEE 198
           AD   V++GI+     R+PVLTPN++GF+AAVAAGA EVA+F SASE+FS+ NINCSIEE
Sbjct: 96  ADHTAVLKGIKRSPDVRYPVLTPNIQGFQAAVAAGANEVAVFGSASETFSRKNINCSIEE 155

Query: 199 SLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDT 258
           SL R+ QV  AA++  +PVRGYVSC +GCP EG V  S V  VAK L+ +GC E+SLGDT
Sbjct: 156 SLQRFEQVVSAAKQEGIPVRGYVSCALGCPYEGQVKPSQVTKVAKRLFELGCYEVSLGDT 215

Query: 259 IGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILVSLQMGISTVDSSVSGLGG 318
           IGVGT G++  ML  V+  VP   LAVH HDTYGQAL NIL++LQMG+S VD+SV+GLGG
Sbjct: 216 IGVGTAGSMAEMLSDVLTEVPAGALAVHCHDTYGQALPNILIALQMGVSVVDASVAGLGG 275

Query: 319 CPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPSASKVTIALR 377
           CP+AKGASGNV+TED++YML+G+G++T VDL K+M AGDFICK L R + SKV+ A R
Sbjct: 276 CPFAKGASGNVSTEDLLYMLHGLGIETGVDLLKVMEAGDFICKALNRKTNSKVSQATR 333

BLAST of PI0005873 vs. ExPASy TrEMBL
Match: A0A0A0LHM6 (Hydroxymethylglutaryl-CoA lyase OS=Cucumis sativus OX=3659 GN=Csa_3G823050 PE=3 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 1.3e-205
Identity = 372/392 (94.90%), Postives = 374/392 (95.41%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAKLSAK TRQ F WRHAYNLSMETPT FLVSPSATDVGV+SIR  GI+REF NCSQQ
Sbjct: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRE KGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADA  VMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV NVVP ENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           K LGRPSASKV IALRKVASN NKCTATASKL
Sbjct: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392

BLAST of PI0005873 vs. ExPASy TrEMBL
Match: A0A5A7UJY0 (Hydroxymethylglutaryl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007370 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 6.9e-202
Identity = 368/392 (93.88%), Postives = 372/392 (94.90%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAKLSAK TRQ F WRHAYNLSM+T    LVSPSA DVGVNSI   GI+REF NCSQQ
Sbjct: 1   MQAAKLSAKFTRQAFSWRHAYNLSMKT----LVSPSAADVGVNSITTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRE KGGLSQ LCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLV SG
Sbjct: 61  DGRDAVLSRENKGGLSQNLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVFSG 120

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKDVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV+NVVPIENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVINVVPIENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDLEKLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLEKLMLAGDFIC 360

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           KHLGRPSASKV IALRKV SNANKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVTSNANKCTATASKL 388

BLAST of PI0005873 vs. ExPASy TrEMBL
Match: A0A1S3B7B2 (Hydroxymethylglutaryl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103486948 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 6.9e-202
Identity = 368/392 (93.88%), Postives = 372/392 (94.90%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAKLSAK TRQ F WRHAYNLSM+T    LVSPSA DVGVNSI   GI+REF NCSQQ
Sbjct: 1   MQAAKLSAKFTRQAFSWRHAYNLSMKT----LVSPSAADVGVNSITTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRE KGGLSQ LCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLV SG
Sbjct: 61  DGRDAVLSRENKGGLSQNLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVFSG 120

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKDVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV+NVVPIENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVINVVPIENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDLEKLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLEKLMLAGDFIC 360

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           KHLGRPSASKV IALRKV SNANKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVTSNANKCTATASKL 388

BLAST of PI0005873 vs. ExPASy TrEMBL
Match: A0A6J1F7V0 (Hydroxymethylglutaryl-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111441653 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 3.1e-186
Identity = 343/392 (87.50%), Postives = 357/392 (91.07%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAK+SAKLTR+G  WRH YN +M   +RFL S SA+ VGV+S   LGI+ E DNC QQ
Sbjct: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DG D VLSRE K  LSQYL  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 61  DGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           L VVEATSFVSPKWVPQLADAN+VMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 121 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESVV+VVPIENLAVHFHDTYGQALSNIL+
Sbjct: 241 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVD SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFI 
Sbjct: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           KHLGRPSASKV IAL+KVAS  NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 392

BLAST of PI0005873 vs. ExPASy TrEMBL
Match: A0A6J1J8Q9 (Hydroxymethylglutaryl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111482306 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.6e-185
Identity = 342/392 (87.24%), Postives = 357/392 (91.07%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAK+SAKLTR+G  WRH YN +M   +RFL S SA+ VGV+S   LGI+ E  NC QQ
Sbjct: 2   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVHNCCQQ 61

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DG DAVLSRE K  LSQYL  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 62  DGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 121

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           L +VEATSFVSPKWVPQLADAN+VMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 122 LRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 181

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESL RYR+VA AARK SLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 182 ASASESFSKSNINCSIEESLRRYREVAVAARKQSLPVRGYVSCVVGCPIEGMVPPSNVAY 241

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVV+VVPIENLAVHFHDTYGQALSNIL+
Sbjct: 242 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 301

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVD SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFI 
Sbjct: 302 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 361

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           KHLGRPSASKV IAL+KVAS  NKCTATASKL
Sbjct: 362 KHLGRPSASKVAIALQKVASKPNKCTATASKL 393

BLAST of PI0005873 vs. NCBI nr
Match: XP_038905646.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 726.1 bits (1873), Expect = 1.6e-205
Identity = 370/392 (94.39%), Postives = 378/392 (96.43%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQ AKLSAKLTRQGF WR+AYNLSMETP RFLV P ATDVGVNSIRRLGI+RE  NCSQQ
Sbjct: 1   MQTAKLSAKLTRQGFSWRYAYNLSMETPCRFLVPPLATDVGVNSIRRLGIRREVGNCSQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRE KGGLSQYLC IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LP+VEATSFVSPKWVPQLADA +VMEGI+NVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPIVEATSFVSPKWVPQLADAKDVMEGIQNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYR+VADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYREVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYRMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKT+VDLEKLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTDVDLEKLMLAGDFIC 360

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           KHLGRPSASKV IALRKVASNANKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVASNANKCTATASKL 392

BLAST of PI0005873 vs. NCBI nr
Match: XP_011652210.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus] >XP_011652211.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus] >XP_031738871.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus])

HSP 1 Score: 725.3 bits (1871), Expect = 2.8e-205
Identity = 372/392 (94.90%), Postives = 374/392 (95.41%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAKLSAK TRQ F WRHAYNLSMETPT FLVSPSATDVGV+SIR  GI+REF NCSQQ
Sbjct: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRE KGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADA  VMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV NVVP ENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           K LGRPSASKV IALRKVASN NKCTATASKL
Sbjct: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392

BLAST of PI0005873 vs. NCBI nr
Match: XP_008443338.1 (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Cucumis melo] >KAA0053821.1 hydroxymethylglutaryl-CoA lyase [Cucumis melo var. makuwa] >TYK25580.1 hydroxymethylglutaryl-CoA lyase [Cucumis melo var. makuwa])

HSP 1 Score: 713.0 bits (1839), Expect = 1.4e-201
Identity = 368/392 (93.88%), Postives = 372/392 (94.90%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAKLSAK TRQ F WRHAYNLSM+T    LVSPSA DVGVNSI   GI+REF NCSQQ
Sbjct: 1   MQAAKLSAKFTRQAFSWRHAYNLSMKT----LVSPSAADVGVNSITTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRE KGGLSQ LCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLV SG
Sbjct: 61  DGRDAVLSRENKGGLSQNLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVFSG 120

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKDVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV+NVVPIENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVINVVPIENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDLEKLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLEKLMLAGDFIC 360

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           KHLGRPSASKV IALRKV SNANKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVTSNANKCTATASKL 388

BLAST of PI0005873 vs. NCBI nr
Match: KAE8651197.1 (hypothetical protein Csa_002494 [Cucumis sativus])

HSP 1 Score: 711.1 bits (1834), Expect = 5.4e-201
Identity = 372/418 (89.00%), Postives = 374/418 (89.47%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAKLSAK TRQ F WRHAYNLSMETPT FLVSPSATDVGV+SIR  GI+REF NCSQQ
Sbjct: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRE KGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQ--------------------------LADANNVMEGIRNVEGA 180
           LPVVEATSFVSPKWVPQ                          LADA  VMEGIRNVEGA
Sbjct: 121 LPVVEATSFVSPKWVPQLPCLILPSPDLQLFMCSVFVFEFLVRLADAKEVMEGIRNVEGA 180

Query: 181 RFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHS 240
           RFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHS
Sbjct: 181 RFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHS 240

Query: 241 LPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV 300
           LPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV
Sbjct: 241 LPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV 300

Query: 301 VNVVPIENLAVHFHDTYGQALSNILVSLQMGISTVDSSVSGLGGCPYAKGASGNVATEDV 360
            NVVP ENLAVHFHDTYGQALSNILVSLQMGI TVDSSVSGLGGCPYAKGASGNVATEDV
Sbjct: 301 ANVVPTENLAVHFHDTYGQALSNILVSLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDV 360

Query: 361 VYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPSASKVTIALRKVASNANKCTATASKL 393
           VYMLNGIG KTNVDL KLMLAGDFICK LGRPSASKV IALRKVASN NKCTATASKL
Sbjct: 361 VYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIALRKVASNTNKCTATASKL 418

BLAST of PI0005873 vs. NCBI nr
Match: XP_023526450.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526451.1 hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 663.3 bits (1710), Expect = 1.3e-186
Identity = 344/392 (87.76%), Postives = 358/392 (91.33%), Query Frame = 0

Query: 1   MQAAKLSAKLTRQGFPWRHAYNLSMETPTRFLVSPSATDVGVNSIRRLGIKREFDNCSQQ 60
           MQAAK+SAKLTR+G  WRH YN +M   +RFL S SA+ VGV S   LGI+ E DNC QQ
Sbjct: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVTSTNGLGIQGEVDNCCQQ 60

Query: 61  DGRDAVLSRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DG DAVLSRE K  LSQYL  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 61  DGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           L +VEATSFVSPKWVPQLADAN+VMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 121 LRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVV+VVPIENLAVHFHDTYGQALSNIL+
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300

Query: 301 SLQMGISTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIC 360
           SLQMGI TVD SVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFI 
Sbjct: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360

Query: 361 KHLGRPSASKVTIALRKVASNANKCTATASKL 393
           KHLGRPSASKV IAL+KVAS  NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 392

BLAST of PI0005873 vs. TAIR 10
Match: AT2G26800.1 (Aldolase superfamily protein )

HSP 1 Score: 483.0 bits (1242), Expect = 2.2e-136
Identity = 255/378 (67.46%), Postives = 302/378 (79.89%), Query Frame = 0

Query: 12  RQGFPW-RHAYNLSM-ETPTRFLVSPSATDVGVNSIRRLGIKRE--FDNCSQQDGRDAVL 71
           ++ FPW RH   LS  E   R +     T     ++R     +E  +   S ++G   + 
Sbjct: 63  KESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTSHIS 122

Query: 72  SRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEAT 131
           ++ +KG        IP FVKIVEVGPRDGLQNEK+IVPT+VKV+LI+ LVSSGLPVVEAT
Sbjct: 123 NKISKG--------IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEAT 182

Query: 132 SFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESF 191
           SFVSPKWVPQLADA +VM+ +  ++GAR PVLTPNLKGF+AAV+AGAKEVAIFASASESF
Sbjct: 183 SFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESF 242

Query: 192 SKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYS 251
           S SNINC+IEESL RYR VA AA++HS+PVRGYVSCVVGCP+EG V  S VAYV KELY 
Sbjct: 243 SLSNINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYD 302

Query: 252 MGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILVSLQMGIS 311
           MGC EISLGDTIG+GTPG+V+PMLE+V+ VVP + LAVHFHDTYGQAL+NILVSLQMGIS
Sbjct: 303 MGCFEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGIS 362

Query: 312 TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPS 371
            VDSS++GLGGCPYAKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFI KHLGRP+
Sbjct: 363 IVDSSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPN 422

Query: 372 ASKVTIAL-RKVASNANK 385
            SK  +AL R++ ++A+K
Sbjct: 423 GSKAAVALNRRITADASK 432

BLAST of PI0005873 vs. TAIR 10
Match: AT2G26800.2 (Aldolase superfamily protein )

HSP 1 Score: 483.0 bits (1242), Expect = 2.2e-136
Identity = 255/378 (67.46%), Postives = 302/378 (79.89%), Query Frame = 0

Query: 12  RQGFPW-RHAYNLSM-ETPTRFLVSPSATDVGVNSIRRLGIKRE--FDNCSQQDGRDAVL 71
           ++ FPW RH   LS  E   R +     T     ++R     +E  +   S ++G   + 
Sbjct: 98  KESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTSHIS 157

Query: 72  SRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEAT 131
           ++ +KG        IP FVKIVEVGPRDGLQNEK+IVPT+VKV+LI+ LVSSGLPVVEAT
Sbjct: 158 NKISKG--------IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEAT 217

Query: 132 SFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESF 191
           SFVSPKWVPQLADA +VM+ +  ++GAR PVLTPNLKGF+AAV+AGAKEVAIFASASESF
Sbjct: 218 SFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESF 277

Query: 192 SKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYS 251
           S SNINC+IEESL RYR VA AA++HS+PVRGYVSCVVGCP+EG V  S VAYV KELY 
Sbjct: 278 SLSNINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYD 337

Query: 252 MGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILVSLQMGIS 311
           MGC EISLGDTIG+GTPG+V+PMLE+V+ VVP + LAVHFHDTYGQAL+NILVSLQMGIS
Sbjct: 338 MGCFEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGIS 397

Query: 312 TVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFICKHLGRPS 371
            VDSS++GLGGCPYAKGASGNVATEDVVYMLNG+GV TNVDL KL+ AGDFI KHLGRP+
Sbjct: 398 IVDSSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPN 457

Query: 372 ASKVTIAL-RKVASNANK 385
            SK  +AL R++ ++A+K
Sbjct: 458 GSKAAVALNRRITADASK 467

BLAST of PI0005873 vs. TAIR 10
Match: AT2G26800.3 (Aldolase superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.7e-83
Identity = 165/261 (63.22%), Postives = 196/261 (75.10%), Query Frame = 0

Query: 12  RQGFPW-RHAYNLSM-ETPTRFLVSPSATDVGVNSIRRLGIKRE--FDNCSQQDGRDAVL 71
           ++ FPW RH   LS  E   R +     T     ++R     +E  +   S ++G   + 
Sbjct: 98  KESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTSHIS 157

Query: 72  SRETKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEAT 131
           ++ +KG        IP FVKIVEVGPRDGLQNEK+IVPT+VKV+LI+ LVSSGLPVVEAT
Sbjct: 158 NKISKG--------IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEAT 217

Query: 132 SFVSPKWVPQLADANNVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESF 191
           SFVSPKWVPQLADA +VM+ +  ++GAR PVLTPNLKGF+AAV+AGAKEVAIFASASESF
Sbjct: 218 SFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESF 277

Query: 192 SKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYS 251
           S SNINC+IEESL RYR VA AA++HS+PVRGYVSCVVGCP+EG V  S VAYV KELY 
Sbjct: 278 SLSNINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYD 337

Query: 252 MGCSEISLGDTIGVGTPGTVI 269
           MGC EISLGDTIG+GTPG  +
Sbjct: 338 MGCFEISLGDTIGIGTPGNFV 350

BLAST of PI0005873 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 47.0 bits (110), Expect = 4.1e-05
Identity = 61/301 (20.27%), Postives = 119/301 (39.53%), Query Frame = 0

Query: 83  PPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADAN 142
           P +V+I +   RDG Q+    + +  K+D+ + L   G+ ++EA    + K         
Sbjct: 84  PNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASK--DDFEAVK 143

Query: 143 NVMEGIRNV--EGARFPVLTP----NLK----GFEAAVAAGAKEVAIFASASESFSKSNI 202
            + E + N   E    PV+      N K     +EA   A    +  F + S+   K  +
Sbjct: 144 TIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKL 203

Query: 203 NCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSE 262
             S EE +   R +   AR       G           G      +  +  E+   G + 
Sbjct: 204 KKSKEEVIEIARNMVRFARS-----LGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATT 263

Query: 263 ISLGDTIGVGTPGTVIPMLESV-VNVVPIEN--LAVHFHDTYGQALSNILVSLQMGISTV 322
           +++ DT+G+  P     ++  +  N   I+N  ++ H  +  G + +N L     G   V
Sbjct: 264 LNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQV 323

Query: 323 DSSVSGLGGCPYAKGASGNVATEDVVYMLNGI------GVKTNVDLEKLMLAGDFICKHL 365
           + +++G+G        +GN + E+VV  +         G+ T +D   +++    + ++ 
Sbjct: 324 EVTINGIG------ERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYT 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O810273.1e-13567.46Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 G... [more]
P359151.6e-11068.15Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL... [more]
P975193.0e-10967.00Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus OX=10116 GN=... [more]
P380601.9e-10866.33Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcl... [more]
A8WG577.3e-10864.773-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Danio rerio OX=7955 GN=hmgc... [more]
Match NameE-valueIdentityDescription
A0A0A0LHM61.3e-20594.90Hydroxymethylglutaryl-CoA lyase OS=Cucumis sativus OX=3659 GN=Csa_3G823050 PE=3 ... [more]
A0A5A7UJY06.9e-20293.88Hydroxymethylglutaryl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3B7B26.9e-20293.88Hydroxymethylglutaryl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103486948 PE=3 SV=... [more]
A0A6J1F7V03.1e-18687.50Hydroxymethylglutaryl-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111441653 PE... [more]
A0A6J1J8Q92.6e-18587.24Hydroxymethylglutaryl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111482306 PE=3... [more]
Match NameE-valueIdentityDescription
XP_038905646.11.6e-20594.39hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Benincasa hispida][more]
XP_011652210.12.8e-20594.90hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus] >XP_011652211.1... [more]
XP_008443338.11.4e-20193.88PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Cucumis melo] >K... [more]
KAE8651197.15.4e-20189.00hypothetical protein Csa_002494 [Cucumis sativus][more]
XP_023526450.11.3e-18687.76hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo s... [more]
Match NameE-valueIdentityDescription
AT2G26800.12.2e-13667.46Aldolase superfamily protein [more]
AT2G26800.22.2e-13667.46Aldolase superfamily protein [more]
AT2G26800.33.7e-8363.22Aldolase superfamily protein [more]
AT1G74040.14.1e-0520.272-isopropylmalate synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 80..378
e-value: 5.8E-114
score: 382.4
IPR000891Pyruvate carboxyltransferasePFAMPF00682HMGL-likecoord: 86..358
e-value: 2.2E-55
score: 188.0
IPR000891Pyruvate carboxyltransferasePROSITEPS50991PYR_CTcoord: 86..353
score: 35.208355
IPR043594HMG-CoA lyasePANTHERPTHR42738HYDROXYMETHYLGLUTARYL-COA LYASEcoord: 50..384
NoneNo IPR availablePANTHERPTHR42738:SF15HYDROXYMETHYLGLUTARYL-COA LYASEcoord: 50..384
NoneNo IPR availableCDDcd07938DRE_TIM_HMGLcoord: 88..361
e-value: 5.28096E-174
score: 484.204
NoneNo IPR availableSUPERFAMILY51569Aldolasecoord: 81..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005873.1PI0005873.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046951 ketone body biosynthetic process
biological_process GO:0006552 leucine catabolic process
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004419 hydroxymethylglutaryl-CoA lyase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016833 oxo-acid-lyase activity