PI0005738 (gene) Melon (PI 482460) v1

Overview


Name	PI0005738
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Peptide chain release factor 2
Location	chr02: 405292 .. 410032 (+)
RNA-Seq Expression	PI0005738
Synteny	PI0005738

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCATTTTCACCTTCAGAAAACAAGTGTGCAATCGAATCTTCACCCTCTTCAGAAACGTATTGATCTCCAGGCATCGGTTTATCCCAGAACTTCAATGTTCATCAGCTACTGCTCAAAAGTTTGCCCTTTCGAATCATGGGATAGCTGATTTTAACAAGATTTTTGGGTTCCCCTTTACCACATTGCGCTTGTTTTCTACACAGATTGCGTTTGAACCCTCAACGTCTGATGGGCTTACTGTCGAAGGAATCCTTGGAAATCGATGGACGGTTCTGGACGAGAGCGAGAGTGATTGGAGGAGCCATGCTGCTGCAATTGCCCAATCCATTCATTTGATCAAGAAGCGTTTACAGGTTTTAAACATGATTTCCCATGTCTTTATTTTTCAACTAATTTCTGCTTTACGGCTTTTTTTTTCTTTCAAGTTTTAGTTTGATGTAAATCAGTTGTGTTATCAAAACGCTTGAAGTTTGATTATCGTTCTTGTATTGGCGATGGCAGTGGAAGAAGCTGATGATGAGATTGGAGTTGTTATCAGTGGAGTTGAATAAGGCTGATTTGTGGGATGATCCTGCACATGCAGGGAAGATAAGTCGGGAGCATGGTTCTATTATGAGTAAGATAAAAGAGATCAACGCATTCGAGAGAGAATTGCTTGAGCATATCGACATGATAAAGCTTGCCAGAGAAGAGAATGATACAGAGTTGGAATCGGTGGGCTCTAAATTCATCTTCTAGGCATGTATTCTCTCTTTATGTTTTTGTTGTTTTTAAATGTGTTCAATTTTGTGCATCCCTTCCTCTTAGTATGTGAGGTAAATTATGCAGTTTGTCGTTCCATAATCAAGAACAGAGTAAATAAGCATACCCTGAACATGGTATTTGGTTTGAAAGAAATGCAAATAGATATCTTTAGTAAGCCCCGTGGTAAAAACTTGGGATACTTGGAGATTGGAGTTCTGATAAAGCTCCCAAGCCCGAAACTAAAGTTTTCCATTTTGGTGTATATTTTGACAGAGATTGCTATTATTCAATATTTTAAATTGCAGCCTTGAGGAACCCCTTGAAAAGGCGACATGAGGCAAAAGCATCAATTTTTATTAGATAGGTTACACAAATCCTAATTTTGTGTGATGATAGTTATACAAAATTTGGAAAAAATACCCCTGTCAATTCCCATGTGAACAAAAATTCTAAAATAAAGGGGTCTTTAGCCTCACTGTAAGTGTAATAGTTCAAATTATTGCAATACAACTCAAACCTTTATGCTTTTGAAGTCTATGGGGTAAATTAATGGTAAAAAAGTACTTGTCTGAAAATTGTCTTATGAGGCATAAGCCTCAATGTGTATACTATACACAACAGAGGTTCAAAGAAGGCATACATGTTGAAGCCAAGCTAAAAATTAAAGCTTACCATCAAGGTGTAACCCCCGATAGTCATTGCTGTTTTTATTTCTTTGTGTAACTCTCAGGCTGTCATCTCGTCTCAATGAGACAAATGCTAATTTTCTTTTGAAATTAGAACAATTTTGGTAGGGAGTAGACAGATCTTTTTCTTTTGTTTTAATCCTGTATTATATGCATTGCCTTTTGCAGGAGTCACTAAATGCTCTACTCACAATGAGAAGAAATTCGAAAGAGAAAGAGCTTGAAGCTTTGTTATCTGGGGAGAACGATTCTTGCTCTTGTTACATTGAGGTCTGCTTAATTTGGTGCTGCTAATTTTTGTCTCTCTTGTGAATTTTATTGTATGTAATATTACTTTTTTCATTGCCGGATTCAAGTTTTGAGAAATGTTAATAGTGATTTGTATAGAGATACAGCCATCAAGCTTTTGAATCTTTGTTGGTCAAACAGGTCCAAGCTGGAGCTGGTGGAACTGAGAGCATGGACTGGGCAGCAATGGTCATGCAGATGTACATAATGTGGGCTCAAAGACGTGGTTTCAAGGTTACTGTTATGGAAGAAATGCCGGGTGAGATTGCAGGAATCAAGGTTCAATCTCTGCTAACTTCAATTGTATATTTCTTCATTGTGTTATCTCACAAAAAATGAGTGGGAGCTCAAATCTGCATGCGCTTGTTACTGCTAGCTAATATAATGTAATGTTTAGCAGTTGAAAATTCTTATGTCATTTAATCCTATTAATTGTATAGCTTGAGTTAAAATCGTCATATCTACTGTATTCACACTCTGGCTTGAGTGATAAAATGCCGGTTCTGACAAATTATTCTGTTATTCCAGCGAGCAACAATTAAGTTAGATGGTGAATATGCTTTTGGATATGCCAAATCGGAAGTGGGCGTACATAGATTGGTTCGCATCTCTCCTTTTGATAGTAACAAGCGTCGACATACTTCGTTTGCTGCTGTAGCTGTCATTCCAATAACTGGTGATGGTTCAATTCATGTTCAAATTAATGAATCAGATCTACGAATTGAGCGTTTCCGGTCTGGAGGAGCTGGTGGTCAGAGTGTTAACACCACCGATAGTGCCGTGAGGATAGTCCATATACCTACAGGGATTACAGCAACTTGTCAAAATGAAAGGTAAGGAGATTTAAAACTGGTTCATTTGATGAATACAACCACTGCTAGATCATTTCTCATTGAAATGGAAAATAACACAGATCACAACATCTAAACAAGGCCTCAGCAATGGCAGTACTCCAGTCTCGATTGAATCAGCTTGAGATGACACGGCAAGCTCAGATAAATGCACAGCATACGCAGAGTCTATCTGATATAACTTGGGGAAACCAAATTCGTAGTTATGTGCTCCATGTGAGTTGTTTTAAACGTAGTGTACTTTCAATTTACCTCGTGTAGTCAGCACTAACTAGTAACTACCAATTGTTTGATTTTTGCAGCCTTATCAAATGGTAAAAGATCTTCGTACCAATTATGAGGTTTCGGATCCTGATTCGGTGCTTGAGGGGGATCTCGACAGCTTTATTTTGAACTATTTGTCGACATCCTTGGACAAAGATGGAGATCAGCTGTAAAGTTGTACTACGATGGATGGTAATTTAGCAACTGAATTTTGCCAAACTAGATATTTAGAGCATAATGTATTTAACTGGAACAAGGGGAAGAAAAAATTGACATCTTAGTTCGCTTTAAATTAAAATTTGCTCACTCTGTGAAAATTTTGTAAGTCTTTAATGGTTAAATTATAGTTATAGTTCCTAAACTTTCAGGCTTGGGTCTAATATCTTTAAATTCTAAAATGTGTAATAAGTAGTAAGTTCTTAAACTTTGGTTTTTGTGTTTAATGGATATCTAGTTATAATTTG

mRNA sequence

ATGTCCATTTTCACCTTCAGAAAACAAGTGTGCAATCGAATCTTCACCCTCTTCAGAAACGTATTGATCTCCAGGCATCGGTTTATCCCAGAACTTCAATGTTCATCAGCTACTGCTCAAAAGTTTGCCCTTTCGAATCATGGGATAGCTGATTTTAACAAGATTTTTGGGTTCCCCTTTACCACATTGCGCTTGTTTTCTACACAGATTGCGTTTGAACCCTCAACGTCTGATGGGCTTACTGTCGAAGGAATCCTTGGAAATCGATGGACGGTTCTGGACGAGAGCGAGAGTGATTGGAGGAGCCATGCTGCTGCAATTGCCCAATCCATTCATTTGATCAAGAAGCGTTTACAGTGGAAGAAGCTGATGATGAGATTGGAGTTGTTATCAGTGGAGTTGAATAAGGCTGATTTGTGGGATGATCCTGCACATGCAGGGAAGATAAGTCGGGAGCATGGTTCTATTATGAGTAAGATAAAAGAGATCAACGCATTCGAGAGAGAATTGCTTGAGCATATCGACATGATAAAGCTTGCCAGAGAAGAGAATGATACAGAGTTGGAATCGGAGTCACTAAATGCTCTACTCACAATGAGAAGAAATTCGAAAGAGAAAGAGCTTGAAGCTTTGTTATCTGGGGAGAACGATTCTTGCTCTTGTTACATTGAGGTCCAAGCTGGAGCTGGTGGAACTGAGAGCATGGACTGGGCAGCAATGGTCATGCAGATGTACATAATGTGGGCTCAAAGACGTGGTTTCAAGGTTACTGTTATGGAAGAAATGCCGGGTGAGATTGCAGGAATCAAGCGAGCAACAATTAAGTTAGATGGTGAATATGCTTTTGGATATGCCAAATCGGAAGTGGGCGTACATAGATTGGTTCGCATCTCTCCTTTTGATAGTAACAAGCGTCGACATACTTCGTTTGCTGCTGTAGCTGTCATTCCAATAACTGGTGATGGTTCAATTCATGTTCAAATTAATGAATCAGATCTACGAATTGAGCGTTTCCGGTCTGGAGGAGCTGGTGGTCAGAGTGTTAACACCACCGATAGTGCCGTGAGGATAGTCCATATACCTACAGGGATTACAGCAACTTGTCAAAATGAAAGATCACAACATCTAAACAAGGCCTCAGCAATGGCAGTACTCCAGTCTCGATTGAATCAGCTTGAGATGACACGGCAAGCTCAGATAAATGCACAGCATACGCAGAGTCTATCTGATATAACTTGGGGAAACCAAATTCGTAGTTATGTGCTCCATCCTTATCAAATGGTAAAAGATCTTCGTACCAATTATGAGGTTTCGGATCCTGATTCGGTGCTTGAGGGGGATCTCGACAGCTTTATTTTGAACTATTTGTCGACATCCTTGGACAAAGATGGAGATCAGCTGTAAAGTTGTACTACGATGGATGGTAATTTAGCAACTGAATTTTGCCAAACTAGATATTTAGAGCATAATGTATTTAACTGGAACAAGGGGAAGAAAAAATTGACATCTTAGTTCGCTTTAAATTAAAATTTGCTCACTCTGTGAAAATTTTGTAAGTCTTTAATGGTTAAATTATAGTTATAGTTCCTAAACTTTCAGGCTTGGGTCTAATATCTTTAAATTCTAAAATGTGTAATAAGTAGTAAGTTCTTAAACTTTGGTTTTTGTGTTTAATGGATATCTAGTTATAATTTG

Coding sequence (CDS)

ATGTCCATTTTCACCTTCAGAAAACAAGTGTGCAATCGAATCTTCACCCTCTTCAGAAACGTATTGATCTCCAGGCATCGGTTTATCCCAGAACTTCAATGTTCATCAGCTACTGCTCAAAAGTTTGCCCTTTCGAATCATGGGATAGCTGATTTTAACAAGATTTTTGGGTTCCCCTTTACCACATTGCGCTTGTTTTCTACACAGATTGCGTTTGAACCCTCAACGTCTGATGGGCTTACTGTCGAAGGAATCCTTGGAAATCGATGGACGGTTCTGGACGAGAGCGAGAGTGATTGGAGGAGCCATGCTGCTGCAATTGCCCAATCCATTCATTTGATCAAGAAGCGTTTACAGTGGAAGAAGCTGATGATGAGATTGGAGTTGTTATCAGTGGAGTTGAATAAGGCTGATTTGTGGGATGATCCTGCACATGCAGGGAAGATAAGTCGGGAGCATGGTTCTATTATGAGTAAGATAAAAGAGATCAACGCATTCGAGAGAGAATTGCTTGAGCATATCGACATGATAAAGCTTGCCAGAGAAGAGAATGATACAGAGTTGGAATCGGAGTCACTAAATGCTCTACTCACAATGAGAAGAAATTCGAAAGAGAAAGAGCTTGAAGCTTTGTTATCTGGGGAGAACGATTCTTGCTCTTGTTACATTGAGGTCCAAGCTGGAGCTGGTGGAACTGAGAGCATGGACTGGGCAGCAATGGTCATGCAGATGTACATAATGTGGGCTCAAAGACGTGGTTTCAAGGTTACTGTTATGGAAGAAATGCCGGGTGAGATTGCAGGAATCAAGCGAGCAACAATTAAGTTAGATGGTGAATATGCTTTTGGATATGCCAAATCGGAAGTGGGCGTACATAGATTGGTTCGCATCTCTCCTTTTGATAGTAACAAGCGTCGACATACTTCGTTTGCTGCTGTAGCTGTCATTCCAATAACTGGTGATGGTTCAATTCATGTTCAAATTAATGAATCAGATCTACGAATTGAGCGTTTCCGGTCTGGAGGAGCTGGTGGTCAGAGTGTTAACACCACCGATAGTGCCGTGAGGATAGTCCATATACCTACAGGGATTACAGCAACTTGTCAAAATGAAAGATCACAACATCTAAACAAGGCCTCAGCAATGGCAGTACTCCAGTCTCGATTGAATCAGCTTGAGATGACACGGCAAGCTCAGATAAATGCACAGCATACGCAGAGTCTATCTGATATAACTTGGGGAAACCAAATTCGTAGTTATGTGCTCCATCCTTATCAAATGGTAAAAGATCTTCGTACCAATTATGAGGTTTCGGATCCTGATTCGGTGCTTGAGGGGGATCTCGACAGCTTTATTTTGAACTATTTGTCGACATCCTTGGACAAAGATGGAGATCAGCTGTAA

Protein sequence

MSIFTFRKQVCNRIFTLFRNVLISRHRFIPELQCSSATAQKFALSNHGIADFNKIFGFPFTTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLAREENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKDGDQL

Homology

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BLAST of PI0005738 vs. ExPASy Swiss-Prot
Match: F4I532 (Peptide chain release factor PrfB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PRFB2 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 5.3e-182
Identity = 332/453 (73.29%), Postives = 382/453 (84.33%), Query Frame = 0


Query: 20  NVLISRHRFIPELQCSSATAQKFALSNHG-IADFNKIFG--------FPFTTLRLFSTQI 79
           +VL+SR  F      SS  A  +   N+G I++ N+ FG        F  +T  L S  +
Sbjct: 33  SVLVSRRTF------SSTPALSYFGFNYGKISNLNQRFGNYAYSGSSFASSTRSLSSEAV 92

Query: 80  AFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQWKKLMMRLELL 139
           A   +T DGLTVE I+ N+W +LDE+E DW+SHAAAIAQSI +IK+RLQWKKL++RL++L
Sbjct: 93  AV-AATCDGLTVERIIANQWPILDENEGDWKSHAAAIAQSIQVIKRRLQWKKLLVRLKVL 152

Query: 140 SVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLAREENDTELES 199
           S ELNK+DLWDDP HAGKISREHGS+  K+K +  FERELLEHIDM+KLA+EEND+ELES
Sbjct: 153 SAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDMLKLAKEENDSELES 212

Query: 200 ESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQ 259
           E+L AL+ MRR SKEKELEALLS +ND CSCYIEVQAGAGGTES DWAAMVM+MY  WAQ
Sbjct: 213 ETLKALIDMRRVSKEKELEALLSADNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQ 272

Query: 260 RRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSF 319
           RR F VTV++E PGEIAGIKRATIK++GEYA+GYAK+EVGVHRLVRISPFDS KRRHTSF
Sbjct: 273 RRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSF 332

Query: 320 AAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQN 379
           AAVAVIPI GDGS  V+IN+SDLRIERFRSGGAGGQ  NTTDSAVRIVHIPTGITATCQN
Sbjct: 333 AAVAVIPILGDGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQN 392

Query: 380 ERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKD 439
           ERSQH NKASAMAVLQSRL+QLEM RQ  +NAQHTQSL++I+WGNQIR+YVLHPY+MVKD
Sbjct: 393 ERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQIRTYVLHPYRMVKD 452

Query: 440 LRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 464
           LRTNYEVSDPDSVLEGDLD FIL++LS+SLDKD
Sbjct: 453 LRTNYEVSDPDSVLEGDLDGFILSFLSSSLDKD 478

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BLAST of PI0005738 vs. ExPASy Swiss-Prot
Match: Q2W3F8 (Peptide chain release factor 2 OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=prfB PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 8.2e-90
Identity = 182/371 (49.06%), Postives = 243/371 (65.50%), Query Frame = 0


Query: 101 RSHAAAIAQSIH----LIKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSI 160
           R+   A+AQ I     L+K+ L W + +MRL+ L+      DLW+D   A KI RE   +
Sbjct: 2   RAEIEALAQDIRRSAALLKRHLNWDEALMRLDELNASAENPDLWNDAGAAQKIMRERNEL 61

Query: 161 MSKIKEINAFERELLEHIDMIKLAREENDTELESESLNALLTMRRNSKEKELEALLSGEN 220
            S I+   A EREL +  ++I+L   E D  +  ++   +  ++  + + ELE LLSGE 
Sbjct: 62  DSAIQGCRALERELADLAELIELGEMEGDQTVIDDAEEQVRALKERAAKMELETLLSGEA 121

Query: 221 DSCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKL 280
           D   CY+E+ AGAGGTES DWA M+++MY  WA++ G+KV  +EE  GE AGIK ATI++
Sbjct: 122 DHNDCYMEINAGAGGTESQDWAEMLLRMYTRWAEKHGYKVEWLEESAGEQAGIKSATIRI 181

Query: 281 DGEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIE 340
            G  A+G+ K+E GVHRLVRISP+DS  RRHTSF++  V P+  D +I +QINES+ RI+
Sbjct: 182 LGHNAYGWLKTESGVHRLVRISPYDSAARRHTSFSSAWVYPVI-DDTIDIQINESECRID 241

Query: 341 RFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEM-T 400
            +R+ GAGGQ +N TDSAVRI HIPTGI   CQ ERSQH N+A A  +L++RL + E+  
Sbjct: 242 TYRASGAGGQHINKTDSAVRITHIPTGIAVACQMERSQHQNRARAWDMLRARLYEAELQK 301

Query: 401 RQAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNY 460
           R+A   A   Q  +DI WG+QIRSYVL PYQMVKDLRTN E SD   VL+GDLD F+   
Sbjct: 302 REAAAQALEDQK-TDIGWGHQIRSYVLQPYQMVKDLRTNVETSDTQGVLDGDLDMFMAAS 361

Query: 461 LSTSLDKDGDQ 467
           L+  +    DQ
Sbjct: 362 LAARVQGQVDQ 370

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BLAST of PI0005738 vs. ExPASy Swiss-Prot
Match: Q165J6 (Peptide chain release factor 2 OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) OX=375451 GN=prfB PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.7e-87
Identity = 179/368 (48.64%), Postives = 250/368 (67.93%), Query Frame = 0


Query: 98  SDWRSHAAAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM 157
           +D ++    I +S+ L+ +RL  +    RLE  +  +    LWDDP  A K+ RE   ++
Sbjct: 3   ADVQNTIEKIQKSLDLLAQRLDVETAPYRLEEFNARVEDPTLWDDPDAAQKLMRERQMLV 62

Query: 158 SKIKEINAFERELLEHIDMIKLAREENDTELESESLNALLTMRRNSKEKELEALLSGEND 217
             +   ++ ++E+ ++I++I+L   E+D ++ S++ NAL  +   + +KELEALL GE D
Sbjct: 63  DAMATHDSIKQEMADNIELIELGEMEDDADVVSDAENALRALEETAAKKELEALLDGEAD 122

Query: 218 SCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLD 277
           S   ++EV AGAGGTES DWA+M+ +MY+ WA+++G+KV +     G+ AGIK AT K+ 
Sbjct: 123 SNDTFLEVNAGAGGTESCDWASMLARMYVRWAEKKGYKVELQSMSDGDEAGIKSATYKIT 182

Query: 278 GEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIER 337
           G  A+G+ KSE GVHRLVRISPFDS  +RHTSF++V V P+  D +I +++N +D+RI+ 
Sbjct: 183 GLNAYGWLKSESGVHRLVRISPFDSAAKRHTSFSSVWVYPVV-DDNIDIEVNPADIRIDT 242

Query: 338 FRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQ 397
           +RS GAGGQ VNTTDSAVRI H PTGI  T  +E+SQH N+  AM  L+SRL QLE+ R+
Sbjct: 243 YRSSGAGGQHVNTTDSAVRITHHPTGIVVT-SSEKSQHQNRDIAMKALKSRLYQLELDRR 302

Query: 398 -AQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYL 457
            A IN  H ++  D  WGNQIRSYVL PYQMVKDLRTN+E SD   VL+GDLD F+   L
Sbjct: 303 NAAINEAH-ENKGDAGWGNQIRSYVLQPYQMVKDLRTNFETSDTKGVLDGDLDGFMAATL 362

Query: 458 STSLDKDG 465
             +LD  G
Sbjct: 363 --ALDASG 365

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BLAST of PI0005738 vs. ExPASy Swiss-Prot
Match: Q1GIB5 (Peptide chain release factor 2 OS=Ruegeria sp. (strain TM1040) OX=292414 GN=prfB PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.7e-85
Identity = 167/349 (47.85%), Postives = 236/349 (67.62%), Query Frame = 0


Query: 105 AAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIN 164
           A I +S+ L+ +R+ W+    RLE  +  +   +LWDDPA A K+ R+  S++  +    
Sbjct: 10  ADIEKSLDLLAQRMNWETAEFRLEEFNARVEDPNLWDDPAAAQKLMRDRQSLVDAMDTYK 69

Query: 165 AFERELLEHIDMIKLAREENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIE 224
           +  ++L + +++I+L   E D E+  ++ + L  ++  + +KELEALL GE D    ++E
Sbjct: 70  SIRQDLQDSVELIELGEMEEDAEVVKDAEDTLKALKEKAAQKELEALLDGETDPNDAFLE 129

Query: 225 VQAGAGGTESMDWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGY 284
           ++AGAGGTE+ DWA+M+ +MY+ WA+ RGF V + EE  G  AGIK AT ++ G  A+G+
Sbjct: 130 IKAGAGGTEACDWASMLARMYVRWAESRGFSVELQEEQAGAEAGIKSATYQIKGHNAYGW 189

Query: 285 AKSEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAG 344
            KSE G HRLVRISPF     R TSFAAV   P+  D +I +++N +D+RI+ +RS GAG
Sbjct: 190 LKSESGTHRLVRISPFGKG-TRETSFAAVGAYPVI-DDNIEIEVNPADIRIDTYRSSGAG 249

Query: 345 GQSVNTTDSAVRIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQ-AQINAQ 404
           GQ VNTTDSAVRI H PTGI  T  +E+SQH N+  AM  L+SRL QLE+ R+ A++NA 
Sbjct: 250 GQHVNTTDSAVRITHEPTGIVVT-SSEKSQHQNRDIAMKALKSRLYQLELDRRNAEVNAL 309

Query: 405 HTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFI 453
           H ++  D  WGNQIRSYV+ PYQMVKDLRTN+E +D   VL+GDLD F+
Sbjct: 310 H-EAKGDAGWGNQIRSYVMQPYQMVKDLRTNHETADTKGVLDGDLDGFM 354

[Close]

BLAST of PI0005738 vs. ExPASy Swiss-Prot
Match: C5CGS4 (Peptide chain release factor 2 OS=Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1) OX=521045 GN=prfB PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.4e-84
Identity = 174/353 (49.29%), Postives = 234/353 (66.29%), Query Frame = 0


Query: 114 IKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEH 173
           IK  +   K+  +L  +  EL+   +W D   AGK+ ++  ++ S++  +N  + EL E+
Sbjct: 20  IKTTVDVDKIKEKLSRIEKELSDPSVWSDQRRAGKLGQQAQALRSQLDLLNKVQ-ELFEN 79

Query: 174 IDMIKLAREENDTELESESLNALLTMRRNS-KEKELEALLSGENDSCSCYIEVQAGAGGT 233
           ID+     EE+++ L  E+LN LL    +  KE EL  LLS   D+ + Y+ V  GAGGT
Sbjct: 80  IDIAVELSEEDESYL--ENLNELLKEAEDMVKEFELNILLSSPYDAYNAYLSVHPGAGGT 139

Query: 234 ESMDWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVH 293
           ES DWA+M+++MYI WA+R  +KVT +EE PGE AGIK ATI + G YA+G  K E GVH
Sbjct: 140 ESQDWASMLLRMYIRWAERNNYKVTTIEEQPGEEAGIKSATINIAGPYAYGKLKYEAGVH 199

Query: 294 RLVRISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTD 353
           RLVRISPFD+N RRHTSFA+V+V P   D  + + I   DL+I+ +R+GGAGGQ VN T+
Sbjct: 200 RLVRISPFDANHRRHTSFASVSVFP-EMDDDVEIDIRPEDLKIDTYRAGGAGGQHVNRTE 259

Query: 354 SAVRIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDIT 413
           SAVRI HIPTGI  TCQNERSQH NKA+AM +L+++L +LE+ ++ Q   +      DI 
Sbjct: 260 SAVRITHIPTGIVVTCQNERSQHQNKATAMKILKAKLFELELEKKRQEKMKLMGEQKDIA 319

Query: 414 WGNQIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYL--STSLDKD 464
           WGNQIRSYV  PY MVKD RTNYE  D  +V++G +D FI   L    S+D+D
Sbjct: 320 WGNQIRSYVFQPYTMVKDHRTNYETGDVQAVMDGYIDDFIEKELLFFASIDED 368

[Close]

BLAST of PI0005738 vs. ExPASy TrEMBL
Match: A0A1S4DY60 (peptide chain release factor PrfB2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492513 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 3.5e-253
Identity = 449/467 (96.15%), Postives = 458/467 (98.07%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRNVLISRHRFIPELQCSSATAQKFALSNHGIADFNKIFGFPF 60
           MSIFTFRKQVCNRIFTLFRN+LIS+HRF+PELQ SSATAQ F+L NHGIADFNKI G PF
Sbjct: 1   MSIFTFRKQVCNRIFTLFRNLLISKHRFVPELQYSSATAQNFSLWNHGIADFNKISGLPF 60

Query: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQW 120
           TTLRLFSTQIA EPSTSDGLTVEGILGNRWT+LDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIALEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESLNALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300
           VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF
Sbjct: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGDGS H+QINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHIQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQH NKA+AMAVLQSRLNQLEMTRQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKDGDQL 468
           VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLS SLDKDGDQL
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDKDGDQL 467

[Close]

BLAST of PI0005738 vs. ExPASy TrEMBL
Match: A0A5D3CEK4 (Peptide chain release factor PrfB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004440 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 3.5e-253
Identity = 449/467 (96.15%), Postives = 458/467 (98.07%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRNVLISRHRFIPELQCSSATAQKFALSNHGIADFNKIFGFPF 60
           MSIFTFRKQVCNRIFTLFRN+LIS+HRF+PELQ SSATAQ F+L NHGIADFNKI G PF
Sbjct: 1   MSIFTFRKQVCNRIFTLFRNLLISKHRFVPELQYSSATAQNFSLWNHGIADFNKISGLPF 60

Query: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQW 120
           TTLRLFSTQIA EPSTSDGLTVEGILGNRWT+LDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIALEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESLNALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300
           VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF
Sbjct: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGDGS H+QINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHIQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQH NKA+AMAVLQSRLNQLEMTRQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKDGDQL 468
           VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLS SLDKDGDQL
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDKDGDQL 467

[Close]

BLAST of PI0005738 vs. ExPASy TrEMBL
Match: A0A0A0LVX3 (RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G561950 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.1e-251
Identity = 448/467 (95.93%), Postives = 455/467 (97.43%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRNVLISRHRFIPELQCSSATAQKFALSNHGIADFNKIFGFPF 60
           MSIFTFRKQVCNRIFTL RNV IS+HRFIP LQ SSATAQ FAL N GI DFNKIFGFPF
Sbjct: 1   MSIFTFRKQVCNRIFTLVRNVFISKHRFIPVLQYSSATAQNFALWNRGITDFNKIFGFPF 60

Query: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQW 120
           TTLRLFSTQIAFEPSTSDGLTVEGILGNRWT+LDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESLNALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300
           VMQMYI+WAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF
Sbjct: 241 VMQMYILWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGDGS HVQINESDLRIER+RSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHVQINESDLRIERYRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQH NKA+AMAVLQSRLNQLEMTRQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKDGDQL 468
           VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLS SLD DGDQL
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDNDGDQL 467

[Close]

BLAST of PI0005738 vs. ExPASy TrEMBL
Match: A0A6J1EUC0 (peptide chain release factor PrfB2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111437810 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.7e-236
Identity = 421/468 (89.96%), Postives = 438/468 (93.59%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRN-----VLISRHRFIPELQCSSATAQKFALSNHGIADFNKI 60
           MS F   K++CNR++ LFRN     VLISR R   ELQCSSAT Q + L N G++DF  I
Sbjct: 1   MSTFALGKRLCNRVYALFRNSNHRGVLISRRRLFTELQCSSATPQNYVLFNCGVSDFKNI 60

Query: 61  FGFPFTTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIK 120
            GFPF+TLRLFSTQ+A EPST+DGLTVEGI+GNRWT+L+ESESDWRSHAAAIAQSIHLIK
Sbjct: 61  SGFPFSTLRLFSTQVAIEPSTADGLTVEGIIGNRWTILEESESDWRSHAAAIAQSIHLIK 120

Query: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHID 180
           KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM KIKEI AFERELLEHID
Sbjct: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHID 180

Query: 181 MIKLAREENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 240
           MIKLAREEND ELESESLNALL+MRRNSKEKELEALL+GENDSCSCYIEVQAGAGGTESM
Sbjct: 181 MIKLAREENDAELESESLNALLSMRRNSKEKELEALLAGENDSCSCYIEVQAGAGGTESM 240

Query: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLV 300
           DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLV
Sbjct: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300

Query: 301 RISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360
           RISPFDSNKRRHTSFAAVAVIPITGD S HVQINESDLRIERFRSGGAGGQSVNTTDSAV
Sbjct: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360

Query: 361 RIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGN 420
           RIVHIPTGITATCQNERSQH NKASAMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGN
Sbjct: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQMNAQHTQSLSDITWGN 420

Query: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 464
           QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD
Sbjct: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 468

[Close]

BLAST of PI0005738 vs. ExPASy TrEMBL
Match: A0A6J1JP75 (peptide chain release factor PrfB2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111486461 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 6.2e-234
Identity = 417/468 (89.10%), Postives = 437/468 (93.38%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRN-----VLISRHRFIPELQCSSATAQKFALSNHGIADFNKI 60
           MS F   K++CNR++ LFRN     VLISR R   ELQCSSAT Q + L N G++DF  I
Sbjct: 1   MSSFALGKRLCNRVYALFRNSNHRGVLISRRRLFTELQCSSATPQNYVLFNCGVSDFKNI 60

Query: 61  FGFPFTTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIK 120
            GFPF+TLRLFSTQ+A EPST+DGLTVEGI+GNRWT+L+ESESDWRSHAAAIAQSIHLIK
Sbjct: 61  SGFPFSTLRLFSTQVAIEPSTADGLTVEGIIGNRWTILEESESDWRSHAAAIAQSIHLIK 120

Query: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHID 180
           KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM KIKE+ AFERELLEHID
Sbjct: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEMKAFERELLEHID 180

Query: 181 MIKLAREENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 240
           MIKLAREEND ELESESLNALL+MRRNSKEKELEALL+GENDSCSCYIEVQAGAGGTESM
Sbjct: 181 MIKLAREENDAELESESLNALLSMRRNSKEKELEALLAGENDSCSCYIEVQAGAGGTESM 240

Query: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLV 300
           DWAAMVMQMYIMWAQR GFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLV
Sbjct: 241 DWAAMVMQMYIMWAQRHGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300

Query: 301 RISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360
           RISPFDSNKRRHTSFAAVAVIPI+GD S HVQINE+DLRIERFRSGGAGGQSVNTTDSAV
Sbjct: 301 RISPFDSNKRRHTSFAAVAVIPISGDASTHVQINEADLRIERFRSGGAGGQSVNTTDSAV 360

Query: 361 RIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGN 420
           RIVHIPTGITATCQNERSQH NKASAMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGN
Sbjct: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQMNAQHTQSLSDITWGN 420

Query: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 464
           QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD
Sbjct: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 468

[Close]

BLAST of PI0005738 vs. NCBI nr
Match: XP_016900916.1 (PREDICTED: peptide chain release factor PrfB2, chloroplastic [Cucumis melo] >XP_016900917.1 PREDICTED: peptide chain release factor PrfB2, chloroplastic [Cucumis melo] >KAA0050946.1 peptide chain release factor PrfB2 [Cucumis melo var. makuwa] >TYK10293.1 peptide chain release factor PrfB2 [Cucumis melo var. makuwa])

HSP 1 Score: 883.6 bits (2282), Expect = 7.3e-253
Identity = 449/467 (96.15%), Postives = 458/467 (98.07%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRNVLISRHRFIPELQCSSATAQKFALSNHGIADFNKIFGFPF 60
           MSIFTFRKQVCNRIFTLFRN+LIS+HRF+PELQ SSATAQ F+L NHGIADFNKI G PF
Sbjct: 1   MSIFTFRKQVCNRIFTLFRNLLISKHRFVPELQYSSATAQNFSLWNHGIADFNKISGLPF 60

Query: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQW 120
           TTLRLFSTQIA EPSTSDGLTVEGILGNRWT+LDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIALEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESLNALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300
           VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF
Sbjct: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGDGS H+QINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHIQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQH NKA+AMAVLQSRLNQLEMTRQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKDGDQL 468
           VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLS SLDKDGDQL
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDKDGDQL 467

[Close]

BLAST of PI0005738 vs. NCBI nr
Match: XP_004135582.1 (peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_011659591.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_011659596.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_031738430.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >KGN66015.1 hypothetical protein Csa_007463 [Cucumis sativus])

HSP 1 Score: 878.6 bits (2269), Expect = 2.3e-251
Identity = 448/467 (95.93%), Postives = 455/467 (97.43%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRNVLISRHRFIPELQCSSATAQKFALSNHGIADFNKIFGFPF 60
           MSIFTFRKQVCNRIFTL RNV IS+HRFIP LQ SSATAQ FAL N GI DFNKIFGFPF
Sbjct: 1   MSIFTFRKQVCNRIFTLVRNVFISKHRFIPVLQYSSATAQNFALWNRGITDFNKIFGFPF 60

Query: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQW 120
           TTLRLFSTQIAFEPSTSDGLTVEGILGNRWT+LDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESLNALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300
           VMQMYI+WAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF
Sbjct: 241 VMQMYILWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGDGS HVQINESDLRIER+RSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHVQINESDLRIERYRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQH NKA+AMAVLQSRLNQLEMTRQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKDGDQL 468
           VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLS SLD DGDQL
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDNDGDQL 467

[Close]

BLAST of PI0005738 vs. NCBI nr
Match: XP_038878322.1 (peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida] >XP_038878323.1 peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida] >XP_038878325.1 peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 848.2 bits (2190), Expect = 3.4e-242
Identity = 433/467 (92.72%), Postives = 447/467 (95.72%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRNVLISRHRFIPELQCSSATAQKFALSNHGIADFNKIFGFPF 60
           MSIFTF+K+VCNRIF++F N LISR RFIPELQCSSATAQ   L N G++DFN+I GFPF
Sbjct: 1   MSIFTFKKRVCNRIFSIFTNALISRRRFIPELQCSSATAQSCVLWNRGLSDFNRISGFPF 60

Query: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQW 120
           +TLRLFST +A EPSTSDGLTVEGILGNRWT+LDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  STLRLFSTLVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180
           KKLMMRLE LSVELNKADLWDDPAHAGKISREHGSIMSKIKEI AFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLEFLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIKAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELE ES+NALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELELESINALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300
           VMQMY MWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLVRISPF
Sbjct: 241 VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGD S HVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDASSHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQH NKASAMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHHNKASAMAVLQSRLNQLEMARQAQLNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGD-LDSFILNYLSTSLDKDGDQ 467
           VLHPYQMVKDLRTNYEVSDPDSVLEGD LDSFILNYLSTSLDKDGD+
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLLDSFILNYLSTSLDKDGDE 467

[Close]

BLAST of PI0005738 vs. NCBI nr
Match: XP_022931672.1 (peptide chain release factor PrfB2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 825.9 bits (2132), Expect = 1.8e-235
Identity = 421/468 (89.96%), Postives = 438/468 (93.59%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRN-----VLISRHRFIPELQCSSATAQKFALSNHGIADFNKI 60
           MS F   K++CNR++ LFRN     VLISR R   ELQCSSAT Q + L N G++DF  I
Sbjct: 1   MSTFALGKRLCNRVYALFRNSNHRGVLISRRRLFTELQCSSATPQNYVLFNCGVSDFKNI 60

Query: 61  FGFPFTTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIK 120
            GFPF+TLRLFSTQ+A EPST+DGLTVEGI+GNRWT+L+ESESDWRSHAAAIAQSIHLIK
Sbjct: 61  SGFPFSTLRLFSTQVAIEPSTADGLTVEGIIGNRWTILEESESDWRSHAAAIAQSIHLIK 120

Query: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHID 180
           KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM KIKEI AFERELLEHID
Sbjct: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHID 180

Query: 181 MIKLAREENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 240
           MIKLAREEND ELESESLNALL+MRRNSKEKELEALL+GENDSCSCYIEVQAGAGGTESM
Sbjct: 181 MIKLAREENDAELESESLNALLSMRRNSKEKELEALLAGENDSCSCYIEVQAGAGGTESM 240

Query: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLV 300
           DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLV
Sbjct: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300

Query: 301 RISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360
           RISPFDSNKRRHTSFAAVAVIPITGD S HVQINESDLRIERFRSGGAGGQSVNTTDSAV
Sbjct: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360

Query: 361 RIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGN 420
           RIVHIPTGITATCQNERSQH NKASAMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGN
Sbjct: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQMNAQHTQSLSDITWGN 420

Query: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 464
           QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD
Sbjct: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 468

[Close]

BLAST of PI0005738 vs. NCBI nr
Match: XP_023531900.1 (peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023531901.1 peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 823.5 bits (2126), Expect = 8.9e-235
Identity = 420/468 (89.74%), Postives = 437/468 (93.38%), Query Frame = 0


Query: 1   MSIFTFRKQVCNRIFTLFRN-----VLISRHRFIPELQCSSATAQKFALSNHGIADFNKI 60
           MS F   K++CNR++ LFRN     VLISR R   ELQCSSAT Q + L N G++DF  I
Sbjct: 1   MSTFALGKRLCNRLYALFRNSNHGGVLISRRRLFTELQCSSATPQNYVLFNCGVSDFKNI 60

Query: 61  FGFPFTTLRLFSTQIAFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIK 120
            GFPF+ LRLFSTQ+A EPST+DGLTVEGI+GNRWT+L+ESESDWRSHAAAIAQSIHLIK
Sbjct: 61  SGFPFSALRLFSTQVAIEPSTADGLTVEGIIGNRWTILEESESDWRSHAAAIAQSIHLIK 120

Query: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHID 180
           KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM KIKEI AFERELLEHID
Sbjct: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHID 180

Query: 181 MIKLAREENDTELESESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 240
           MIKLAREEND ELESESLNALL+MRRNSKEKELEALL+GENDSCSCYIEVQAGAGGTESM
Sbjct: 181 MIKLAREENDAELESESLNALLSMRRNSKEKELEALLAGENDSCSCYIEVQAGAGGTESM 240

Query: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLV 300
           DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLV
Sbjct: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300

Query: 301 RISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360
           RISPFDSNKRRHTSFAAVAVIPITGD S HVQINESDLRIERFRSGGAGGQSVNTTDSAV
Sbjct: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360

Query: 361 RIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGN 420
           RIVHIPTGITATCQNERSQH NKASAMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGN
Sbjct: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQMNAQHTQSLSDITWGN 420

Query: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 464
           QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD
Sbjct: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 468

[Close]

BLAST of PI0005738 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 638.6 bits (1646), Expect = 3.8e-183
Identity = 332/453 (73.29%), Postives = 382/453 (84.33%), Query Frame = 0


Query: 20  NVLISRHRFIPELQCSSATAQKFALSNHG-IADFNKIFG--------FPFTTLRLFSTQI 79
           +VL+SR  F      SS  A  +   N+G I++ N+ FG        F  +T  L S  +
Sbjct: 33  SVLVSRRTF------SSTPALSYFGFNYGKISNLNQRFGNYAYSGSSFASSTRSLSSEAV 92

Query: 80  AFEPSTSDGLTVEGILGNRWTVLDESESDWRSHAAAIAQSIHLIKKRLQWKKLMMRLELL 139
           A   +T DGLTVE I+ N+W +LDE+E DW+SHAAAIAQSI +IK+RLQWKKL++RL++L
Sbjct: 93  AV-AATCDGLTVERIIANQWPILDENEGDWKSHAAAIAQSIQVIKRRLQWKKLLVRLKVL 152

Query: 140 SVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLAREENDTELES 199
           S ELNK+DLWDDP HAGKISREHGS+  K+K +  FERELLEHIDM+KLA+EEND+ELES
Sbjct: 153 SAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDMLKLAKEENDSELES 212

Query: 200 ESLNALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQ 259
           E+L AL+ MRR SKEKELEALLS +ND CSCYIEVQAGAGGTES DWAAMVM+MY  WAQ
Sbjct: 213 ETLKALIDMRRVSKEKELEALLSADNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQ 272

Query: 260 RRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSF 319
           RR F VTV++E PGEIAGIKRATIK++GEYA+GYAK+EVGVHRLVRISPFDS KRRHTSF
Sbjct: 273 RRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSF 332

Query: 320 AAVAVIPITGDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQN 379
           AAVAVIPI GDGS  V+IN+SDLRIERFRSGGAGGQ  NTTDSAVRIVHIPTGITATCQN
Sbjct: 333 AAVAVIPILGDGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQN 392

Query: 380 ERSQHLNKASAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKD 439
           ERSQH NKASAMAVLQSRL+QLEM RQ  +NAQHTQSL++I+WGNQIR+YVLHPY+MVKD
Sbjct: 393 ERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQIRTYVLHPYRMVKD 452

Query: 440 LRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 464
           LRTNYEVSDPDSVLEGDLD FIL++LS+SLDKD
Sbjct: 453 LRTNYEVSDPDSVLEGDLDGFILSFLSSSLDKD 478

[Close]

BLAST of PI0005738 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 236.9 bits (603), Expect = 3.3e-62
Identity = 134/377 (35.54%), Postives = 212/377 (56.23%), Query Frame = 0


Query: 91  TVLDESE-SDW--------RSHAAAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWD 150
           +V +E+E S+W        R      +  +  I+     ++L   +  L  +      WD
Sbjct: 66  SVSNENETSEWLMQDFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWD 125

Query: 151 DPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLARE--ENDTELESESLNALLTM 210
           D   A +       +  +++ ++ F+  + +   ++KL  E    D  L  E++  +  +
Sbjct: 126 DRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKEL 185

Query: 211 RRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQRRGFKVTVM 270
            ++  + EL  LLSG  D     + + AGAGGT++ DWA M+++MY+ W +++ +K  V+
Sbjct: 186 NKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVV 245

Query: 271 EEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSFAAVAVIPIT 330
           E   GE AGIK AT++++G YA+GY   E G HR+VR SPF+S   R TSF+ V V+P+ 
Sbjct: 246 EMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLL 305

Query: 331 GDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHLNKA 390
            + ++ ++I E DL I   R+GG GGQ+VN  ++AVRI HIPTG+   C  ERSQ  NK 
Sbjct: 306 PEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKT 365

Query: 391 SAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSD 450
            A+  L+++L  +   ++A    +         WG QIR+YV HPY++VKD+RT +E SD
Sbjct: 366 RALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSD 425

Query: 451 PDSVLEGDLDSFILNYL 457
             SV++GDLD FI  YL
Sbjct: 426 ITSVMDGDLDPFIKAYL 442

[Close]

BLAST of PI0005738 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 236.9 bits (603), Expect = 3.3e-62
Identity = 134/377 (35.54%), Postives = 212/377 (56.23%), Query Frame = 0


Query: 91  TVLDESE-SDW--------RSHAAAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWD 150
           +V +E+E S+W        R      +  +  I+     ++L   +  L  +      WD
Sbjct: 65  SVSNENETSEWLMQDFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWD 124

Query: 151 DPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLARE--ENDTELESESLNALLTM 210
           D   A +       +  +++ ++ F+  + +   ++KL  E    D  L  E++  +  +
Sbjct: 125 DRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKEL 184

Query: 211 RRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQRRGFKVTVM 270
            ++  + EL  LLSG  D     + + AGAGGT++ DWA M+++MY+ W +++ +K  V+
Sbjct: 185 NKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVV 244

Query: 271 EEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSFAAVAVIPIT 330
           E   GE AGIK AT++++G YA+GY   E G HR+VR SPF+S   R TSF+ V V+P+ 
Sbjct: 245 EMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLL 304

Query: 331 GDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHLNKA 390
            + ++ ++I E DL I   R+GG GGQ+VN  ++AVRI HIPTG+   C  ERSQ  NK 
Sbjct: 305 PEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKT 364

Query: 391 SAMAVLQSRLNQLEMTRQAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSD 450
            A+  L+++L  +   ++A    +         WG QIR+YV HPY++VKD+RT +E SD
Sbjct: 365 RALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSD 424

Query: 451 PDSVLEGDLDSFILNYL 457
             SV++GDLD FI  YL
Sbjct: 425 ITSVMDGDLDPFIKAYL 441

[Close]

BLAST of PI0005738 vs. TAIR 10
Match: AT5G36170.3 (high chlorophyll fluorescent 109 )

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-42
Identity = 96/289 (33.22%), Postives = 159/289 (55.02%), Query Frame = 0


Query: 91  TVLDESE-SDW--------RSHAAAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWD 150
           +V +E+E S+W        R      +  +  I+     ++L   +  L  +      WD
Sbjct: 66  SVSNENETSEWLMQDFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWD 125

Query: 151 DPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLARE--ENDTELESESLNALLTM 210
           D   A +       +  +++ ++ F+  + +   ++KL  E    D  L  E++  +  +
Sbjct: 126 DRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKLTEEMDSTDVSLLEEAMGIIKEL 185

Query: 211 RRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQRRGFKVTVM 270
            ++  + EL  LLSG  D     + + AGAGGT++ DWA M+++MY+ W +++ +K  V+
Sbjct: 186 NKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVV 245

Query: 271 EEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSFAAVAVIPIT 330
           E   GE AGIK AT++++G YA+GY   E G HR+VR SPF+S   R TSF+ V V+P+ 
Sbjct: 246 EMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLL 305

Query: 331 GDGSIHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATC 369
            + ++ ++I E DL I   R+GG GGQ+VN  ++AVRI HIPTG+   C
Sbjct: 306 PEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRC 354

[Close]

BLAST of PI0005738 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 154.1 bits (388), Expect = 2.8e-37
Identity = 103/302 (34.11%), Postives = 167/302 (55.30%), Query Frame = 0


Query: 156 IMSKIKEINAFE---RELLEHIDMIKLAREENDTELESESLNALLTMRRNSKEKELEALL 215
           + +K KEI+  +    E  +  DM+ LA  E D  +E E     L ++          L 
Sbjct: 104 LRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAVEEEKRLQTLLLK--------SLLP 163

Query: 216 SGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRA 275
             E D   C +EV+AG GG E+  +A  + +MY  ++Q++G+K  +++    ++ G K A
Sbjct: 164 KDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKFDIVDITESDMKGYKEA 223

Query: 276 TIKLDGEYAFGYAKSEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDGSIHVQINESD 335
           +  + G   +G  K E G+HR+ RI   + + R HTS  +VA++P   +  + VQ+   D
Sbjct: 224 SAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAILPQADE--VDVQLRNED 283

Query: 336 LRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHLNKASAMAVLQSRLNQL 395
           LRI+ +RSGG+GGQ  NTT+SAVRI+H+PTG+  + Q+ERSQH+N+A A+ VL +RL ++
Sbjct: 284 LRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCARLYEI 343

Query: 396 EMTR-QAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGD-LDS 453
           E  R Q+  +   +  +       +IR+Y   P   V D R        + +++G+ LD 
Sbjct: 344 ERLRIQSSRSKLRSDQIGSGDRSGRIRTYNF-PQGRVTDHRVGITHHAIEDMMQGENLDM 394

The following BLAST results are available for this feature:

BLAST of PI0005738 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I532	5.3e-182	73.29	Peptide chain release factor PrfB2, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q2W3F8	8.2e-90	49.06	Peptide chain release factor 2 OS=Magnetospirillum magneticum (strain AMB-1 / AT...	[more]
Q165J6	1.7e-87	48.64	Peptide chain release factor 2 OS=Roseobacter denitrificans (strain ATCC 33942 /...	[more]
Q1GIB5	2.7e-85	47.85	Peptide chain release factor 2 OS=Ruegeria sp. (strain TM1040) OX=292414 GN=prfB...	[more]
C5CGS4	1.4e-84	49.29	Peptide chain release factor 2 OS=Kosmotoga olearia (strain ATCC BAA-1733 / DSM ...	[more]

BLAST of PI0005738 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4DY60	3.5e-253	96.15	peptide chain release factor PrfB2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5D3CEK4	3.5e-253	96.15	Peptide chain release factor PrfB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0LVX3	1.1e-251	95.93	RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G561950 P...	[more]
A0A6J1EUC0	8.7e-236	89.96	peptide chain release factor PrfB2, chloroplastic OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1JP75	6.2e-234	89.10	peptide chain release factor PrfB2, chloroplastic OS=Cucurbita maxima OX=3661 GN...	[more]

BLAST of PI0005738 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016900916.1	7.3e-253	96.15	PREDICTED: peptide chain release factor PrfB2, chloroplastic [Cucumis melo] >XP_...	[more]
XP_004135582.1	2.3e-251	95.93	peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_01165959...	[more]
XP_038878322.1	3.4e-242	92.72	peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida]...	[more]
XP_022931672.1	1.8e-235	89.96	peptide chain release factor PrfB2, chloroplastic [Cucurbita moschata]	[more]
XP_023531900.1	8.9e-235	89.74	peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo] >...	[more]

BLAST of PI0005738 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56350.1	3.8e-183	73.29	Peptide chain release factor 2	[more]
AT5G36170.1	3.3e-62	35.54	high chlorophyll fluorescent 109	[more]
AT5G36170.2	3.3e-62	35.54	high chlorophyll fluorescent 109	[more]
AT5G36170.3	1.3e-42	33.22	high chlorophyll fluorescent 109	[more]
AT2G47020.1	2.8e-37	34.11	Peptide chain release factor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 163..183
None	No IPR available	GENE3D	3.30.70.1660		coord: 223..452 e-value: 9.6E-91 score: 304.7
None	No IPR available	GENE3D	1.20.58.410	Release factor	coord: 98..209 e-value: 1.2E-12 score: 49.7
None	No IPR available	GENE3D	3.30.160.20		coord: 319..415 e-value: 9.6E-91 score: 304.7
None	No IPR available	PANTHER	PTHR43116:SF3	PEPTIDE CHAIN RELEASE FACTOR PRFB1, CHLOROPLASTIC-RELATED	coord: 94..465
None	No IPR available	PANTHER	PTHR43116	PEPTIDE CHAIN RELEASE FACTOR 2	coord: 94..465
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 124..459
IPR005139	Peptide chain release factor	SMART	SM00937	PCRF_a_2	coord: 178..288 e-value: 3.4E-42 score: 156.2
IPR005139	Peptide chain release factor	PFAM	PF03462	PCRF	coord: 126..313 e-value: 1.8E-51 score: 174.7
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 324..432 e-value: 1.1E-35 score: 122.0
IPR000352	Peptide chain release factor class I	PROSITE	PS00745	RF_PROK_I	coord: 339..355
IPR004374	Peptide chain release factor 2	TIGRFAM	TIGR00020	TIGR00020	coord: 99..457 e-value: 5.3E-131 score: 434.8
IPR004374	Peptide chain release factor 2	HAMAP	MF_00094	Rel_fac_2	coord: 96..460 score: 35.942841

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0005738.1	PI0005738.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006415	translational termination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016149	translation release factor activity, codon specific
molecular_function	GO:0003747	translation release factor activity