PI0005671 (gene) Melon (PI 482460) v1

Overview
NamePI0005671
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPseudouridine synthase
Locationchr03: 4920590 .. 4925448 (-)
RNA-Seq ExpressionPI0005671
SyntenyPI0005671
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAAACAGCCGATAAGCGAAAACCCAAAAACGAAAAAATCGAAGACGGGAGAATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTTCTATTCACTTCTTTTTCCCTCGAAATCGTAGAAGAATCACTTCGAATGTTCTTCGTTTTTCCGCCAATTTCTCCACTAGATGCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCTGGAATTAGACTGGAAGAGACCGTGGACATCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATATCTTCCCGCATTAACGGCATTAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTCTCAGTCAATGGCCGAGTTATTGATAAGGTAGCGTCATTTTTCCATAGAGAAGCTACATTTGTTTACTTTTTTTTTTCCTTTCTATTTAGCTTCTTTCAGCTATTTTAGTATGATTATCAAGTGGTCTGTAATCCCGATGGTGAATTATTTTCAGCCTATATGTTCTGTTTTCGTTCTATATTTGAGTTCTTAATTTGTGTGTTCATTTGCAACTTCTATATATTTTTTTAATACTCAAGCAGGTTTCTCACAATGTAAAAGCTGGGGATATGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCCCACATGGTAATGATTCAAAAAAGTTTAATATTGTTATTATCAATACTACTGGAAGTTATTTGTGGATGCAAGATATCGATACTTGTGGATAAAATTAAACGCCCTCCCCTCCCCCGCCTGAAGTTTTTAGTAAGATTGAAATTTGTACGTATCTGTGTTTAGCGGTTTTCTTCATGATTCAGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAACGCCATTCTTAACCACTGCAGTCTCCCAATGGTTGCAACATCAACTGGGGAACATCAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGAAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCCGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGTGATTTGCTCTCCTTTTTTAAGCTTCCTTGTAGTTGTTACTGCTTATCTGAAGATTAAAGCAGACAATTCAAGTTTGGTGCTTCAAAAGTTTACTACTCAAACACTTGTAATATCATGTCTAACTTCTAAGTTTTTTGCATTTGAACTACAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGGCGTATTGAGATACCAATTTGCCGTGATCCAAACAATCGCATCCGCATGACTGCAATCTCTGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGGTAATTAGTTTTTCTGCATCTTTTATTTTATTTATGTACTTAATTATTTTTCAGTGATAGAAAATATTTAAATATCATTAATTTGGGCGTTCCTAATGCAAAGTTGAAACACTGAACGTTTCACAGGTACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACGGGCCGCACACATCAGGTGTGTAGTGTAAATGTCTTTTTCCTTAGGCTGCTTCAAATGACAAAAAAAAAAAAAAAGAATTATATTACAATCCATCAACAATGGCAGTTTAGAACATAAATTATATTCCCTAAACGCCACGTATATGTGCAAAGACATACATTTCTTTTATTTGGAAAAGATTTCTCTATGATACTGCTACTGTATTCCAGTTTTAATGTGTGCATATGTAAAATAAAATTTCAATATAATGAATCAGAGTTACACCTTGAGCATATAAGCAGCCACATTTACCATTCCACATCCGCTGCAATTTCAGTTATTTTCTTTTTTTGAAAATTGAAATGCAGTTTTACTTTACTCGGTCTTAGGAGTGAATATTTGTGAAGTAGATCCGTGCACATGCAAAGTATATGGGAATACCGTTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAACTCATTCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTAGAAAATAAATCTTTAAGAGCTCATTTAGTTACATTCTTATTTCATTTACTATCCATTTTTTGAGAAATATACGTGATTTTGACCTTTCTTCTTTATTTTATTTCATTTTTTCTTTTCTAAAATAGTTTATAATTTTCATCTCTTATTTCTAATGTTTTGAAACTAAATGGAATTTCTAATTATATTATTTCCTCTAATAATTATATGCAAAATATCAAAACATTAATTTGACATATATTCAATTCCAATTTTCAGTTAAAATTTCCACTTTTTGAGAAGAAACGTTGTAGAGAAAAATAAACGACAACGATTGCTAATCACATTTTTGTTCTTTTTTAATAAAAAAAAGGAGAAAAGCAAGAAATGTTATAAAAAACATGTTTTCACTTTTTACAATAAAAGAGACTGCAATCAAAAGTGAGAAACTAGAATAAGATCATTAAAAATGAATCTTTACTTGTTCATTTCTTCCGTAAACAACTAATTCAAGATCACTGAACAATTTATAGGTTTGTACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCAACAGATTTTGCTGAAATTTTGAGCCAGCTTCGTGAAATTGGCACAAAAAAGGTATCCCTTATTTTTATTTCTTTCATTTTATAAGTCGAGCCAAGCTCTTTGCATGGTAATTGGTTATTTTACAGGTTTAGTATTCATCCATCATCATGAAGAACAATATAAAAAATGGCCCGAGGGCTACAATTGACACAACCAACCTCATCCATAGCTACCTAGATAGTTAATACGTGTCACCTTTATGCCTCTTTTCTGGGCGCCCCCTCGCCATGTTATAGCTAATACCAGGTCTTCTCTATTTACTAGCTATATTTCACCTGGTTCTTGCGGTTTGGAGTGACTATTTATTGGTTTACTTTCCTTTTGTAATACATAAAATATATTTTCTAATTATTTAAATGATGTTTTTATACATGAAAACATTGGAGACATGATAAATATAACATAATAACTAAATAGCAGACAAACAAACATAGAAGTTCTCCTTTTACTTGCTTCTCTTATAAATAGTAATGAATATGGACCTTTGTTCTAGCTATTCTAGATTATCCATACAAATGGCATAATGGGGACAAATTCCCTGAACAATTTTAACTCTACATCATCAGGAAATTTCGCATCATATCTTGTGGATTAATCTTATGCAGTTCTAATGGTAGATAGCGCAAATTGAAATAATTTTGAGCTTTACAAACATATTAGATGTTGAACAATGGTTGCTACCATAGAATCTAGGTGAGATGTGGTTTCTACGGTGTTCCAGTTTAACCTATAACAGACTGTTGTCATAAAAGTTGCAGGTTATCAAATCTTAGCTGGGAGCCTCCAGTATCTGGCAAGTAGACTCATCGTCAAATGGCTCGAAAATGAGAGATCAAGAAGGTACATTAAGCCCTAACATACAACCTTTTAAGTTGTGATGTCTGATGCACAAATTTAAGTTCTGGATAACTTACATATTTTGTTTATTAGATTCATTTAATTGTTGAAATTTAAAATATTTTGTGAGTCGCACATTCCCAGACGTTGAATTCCACATATTTTAAATCTCTGCCATTAATATTGATTATTGAAACCTAGACATGACATTCATTTTATTAGAATCAATAATGTAAATACGGGTAATTACTTAGTCGTTACACTATGTACAGTTAATTCTAATACATTTCTTAAAATTTAGATGAAGAGAAATAATTGGATTCTTTCTTCTTGCCACCGCTCCTTTTATTTCATTTCCAACTTCAGCTACCTTTCCGCCTTCTTTTCGCTTCAATTCATAATAAGAATATGCTGATGTTTGGGTTGGTGTGTTTTGCTGTGGCTTTCCTTTGAAAATTCAACTCTCTAAATTTGCTTACAATAATATACACAGGAAATGCTCCGAATGCTCAATAA

mRNA sequence

AAACAAACAGCCGATAAGCGAAAACCCAAAAACGAAAAAATCGAAGACGGGAGAATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTTCTATTCACTTCTTTTTCCCTCGAAATCGTAGAAGAATCACTTCGAATGTTCTTCGTTTTTCCGCCAATTTCTCCACTAGATGCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCTGGAATTAGACTGGAAGAGACCGTGGACATCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATATCTTCCCGCATTAACGGCATTAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTCTCAGTCAATGGCCGAGTTATTGATAAGGTTTCTCACAATGTAAAAGCTGGGGATATGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCCCACATGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAACGCCATTCTTAACCACTGCAGTCTCCCAATGGTTGCAACATCAACTGGGGAACATCAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGAAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCCGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGGCGTATTGAGATACCAATTTGCCGTGATCCAAACAATCGCATCCGCATGACTGCAATCTCTGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGGTACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACGGGCCGCACACATCAGATCCGTGCACATGCAAAGTATATGGGAATACCGTTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAACTCATTCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTTGTACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCAACAGATTTTGCTGAAATTTTGAGCCAGCTTCGTGAAATTGGCACAAAAAAGCTGGGAGCCTCCAGTATCTGGCAAGTAGACTCATCGTCAAATGGCTCGAAAATGAGAGATCAAGAAGGAAATGCTCCGAATGCTCAATAA

Coding sequence (CDS)

ATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTTCTATTCACTTCTTTTTCCCTCGAAATCGTAGAAGAATCACTTCGAATGTTCTTCGTTTTTCCGCCAATTTCTCCACTAGATGCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCTGGAATTAGACTGGAAGAGACCGTGGACATCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATATCTTCCCGCATTAACGGCATTAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTCTCAGTCAATGGCCGAGTTATTGATAAGGTTTCTCACAATGTAAAAGCTGGGGATATGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCCCACATGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAACGCCATTCTTAACCACTGCAGTCTCCCAATGGTTGCAACATCAACTGGGGAACATCAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGAAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCCGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGGCGTATTGAGATACCAATTTGCCGTGATCCAAACAATCGCATCCGCATGACTGCAATCTCTGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGGTACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACGGGCCGCACACATCAGATCCGTGCACATGCAAAGTATATGGGAATACCGTTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAACTCATTCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTTGTACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCAACAGATTTTGCTGAAATTTTGAGCCAGCTTCGTGAAATTGGCACAAAAAAGCTGGGAGCCTCCAGTATCTGGCAAGTAGACTCATCGTCAAATGGCTCGAAAATGAGAGATCAAGAAGGAAATGCTCCGAATGCTCAATAA

Protein sequence

MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQSERIANYAGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLREIGTKKLGASSIWQVDSSSNGSKMRDQEGNAPNAQ
Homology
BLAST of PI0005671 vs. ExPASy Swiss-Prot
Match: Q3ECD0 (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 1.9e-144
Identity = 277/431 (64.27%), Postives = 327/431 (75.87%), Query Frame = 0

Query: 3   SVCSARTIQ--ASPS---SIHF-FFPRNRRRITSNVLRFSANFS--TRCSDYDEYSEAQS 62
           S+ +A++++  +SPS   SI F FFP+      SN +R S+       CS     +E   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK-----VSNFVRASSGIPNLVACSP----TEIII 70

Query: 63  ERIANYAGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVS 122
            R+ N AG+R+EE VD   GK+RLDSWISSRING+SRARVQSSIR GLV+VNGRV+DKVS
Sbjct: 71  PRV-NNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVS 130

Query: 123 HNVKAGDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLV 182
           HNVK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLV
Sbjct: 131 HNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLV 190

Query: 183 NAILNHCSLPMVATSTGEHQSDSD---VSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTS 242
           N IL+HCSLP V  S  E   DSD    SDDE  +    S    A +RPGIVHRLDKGT+
Sbjct: 191 NGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTT 250

Query: 243 GLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAI 302
           GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIEIPI RD +NRIRM AI
Sbjct: 251 GLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAI 310

Query: 303 SGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYG 362
            G      A++AASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYG
Sbjct: 311 PGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYG 370

Query: 363 GTKSMAMSLLRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF 422
           GTKSMA+SLL+ R S +   ++I+L+S ++RPCLHA+ LGF HP TG+ ++FSCPPP+D 
Sbjct: 371 GTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPSDL 429

BLAST of PI0005671 vs. ExPASy Swiss-Prot
Match: Q5Z8P2 (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.3e-137
Identity = 260/436 (59.63%), Postives = 311/436 (71.33%), Query Frame = 0

Query: 7   ARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQS-ERIANYAGIRL 66
           A T  ASP +I        RR      R       RC   D  +EA +  R   + G RL
Sbjct: 2   ATTAAASPPAIATALSALLRRQRRRSSRCVGASHARCLAADANAEAVAPSRRGGHGGTRL 61

Query: 67  EETVDINPGKVRLDSWISSRI--NGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDMI 126
           EE V    G+ R+D+WIS+R+   G+SRAR+Q+SIRAGLV VNGR + KVSH VK GD++
Sbjct: 62  EEAVPAGEGRSRIDAWISARLGGGGVSRARIQASIRAGLVVVNGRPVSKVSHMVKGGDIV 121

Query: 127 NCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSL 186
           +CT+ ELQPLRAEPEDI LDIVYED+H+LVVNKPAHMVVHPAPGNA GTLVNAIL+HC +
Sbjct: 122 SCTVLELQPLRAEPEDIPLDIVYEDDHLLVVNKPAHMVVHPAPGNANGTLVNAILHHCKI 181

Query: 187 P----MVATSTGEHQSDS-----DVSD-DEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGL 246
                +   S  +   DS     DV D D+F++ EV S V EA +RPGIVHRLDKGTSGL
Sbjct: 182 STFTCLARNSIDDECPDSSDDDIDVFDIDQFTTGEVSSEVREALVRPGIVHRLDKGTSGL 241

Query: 247 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISG 306
           LVVAKDEHSHA L+ QFK H+I RVYISLTCG P   +GRIE+PI RDPNNRIRM A  G
Sbjct: 242 LVVAKDEHSHAQLAEQFKLHTIRRVYISLTCGAPNPNSGRIEVPIARDPNNRIRMIATPG 301

Query: 307 TKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGT 366
           +  + +A++AASRY V E+ A GGS+LVEWRLETGRTHQIRAHAKY+GIPLLGDE YGGT
Sbjct: 302 S-GHRYARHAASRYKVREVFAGGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDETYGGT 361

Query: 367 KSMAMSLLRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAE 426
           KSMA+SLLR R  S  H  L  ++S ++RPCLHA  LGF HPH+GK + FSCPPP DF E
Sbjct: 362 KSMALSLLRPRTPSRYHCDLSNMISKIDRPCLHAALLGFKHPHSGKILEFSCPPPDDFTE 421

Query: 427 ILSQLREIGTKKLGAS 430
           +L++L ++     G S
Sbjct: 422 VLNELHQVTLASNGNS 436

BLAST of PI0005671 vs. ExPASy Swiss-Prot
Match: Q45480 (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) OX=224308 GN=ylyB PE=3 SV=3)

HSP 1 Score: 217.2 bits (552), Expect = 3.7e-55
Identity = 134/346 (38.73%), Postives = 185/346 (53.47%), Query Frame = 0

Query: 77  RLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDMINCTISELQPLRAE 136
           R+D +++S  N  SR +VQ  ++ G V VNG  + K ++ ++ GD +  T+ E + L   
Sbjct: 16  RIDKFLASTENDWSRTQVQQWVKDGQVVVNGSAV-KANYKIQPGDQVTVTVPEPEALDVL 75

Query: 137 PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGEHQSD 196
            E +DLDI YED+ VLVVNKP  MVVHPAPG+ TGTLVN ++ HC+              
Sbjct: 76  AEPMDLDIYYEDQDVLVVNKPRGMVVHPAPGHLTGTLVNGLMAHCT-------------- 135

Query: 197 SDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIE 256
                 + S I          +RPGIVHR+DK TSGLL+VAK++ +H  L  Q    ++ 
Sbjct: 136 ------DLSGI-------NGVMRPGIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTVT 195

Query: 257 RVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHAKYAASRYNVMEILAQG 316
           R Y ++  G+     G I+ PI RD  +R  MT     KN      A + ++V+E     
Sbjct: 196 RKYTAVVHGLISHDDGTIDAPIGRDKKDRQSMTVTRDGKN------AVTHFHVLERFQD- 255

Query: 317 GSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLIQL 376
             +LVE +LETGRTHQIR H KY+G PL GD  YG  K++           + +GQ    
Sbjct: 256 -FTLVECQLETGRTHQIRVHMKYIGFPLAGDPKYGPRKTI-----------DFNGQ---- 303

Query: 377 VSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLREIG 423
                   LHA  LGF HP TG+ + F  P P D AE++  LR+ G
Sbjct: 316 -------ALHAGVLGFDHPRTGEYVEFEAPLPEDMAELIENLRKNG 303

BLAST of PI0005671 vs. ExPASy Swiss-Prot
Match: P50513 (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rluD PE=3 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 1.4e-54
Identity = 137/343 (39.94%), Postives = 186/343 (54.23%), Query Frame = 0

Query: 77  RLDSWISSRINGISRARVQSSIRAGLVS-VNGRVIDKVSHNVKAGDMINCTISELQPLRA 136
           RLD  ++S I  +SR R+++ I +G VS   G ++   +  +K+ D     I   +P   
Sbjct: 16  RLDRALASLITRLSRERLKNLISSGCVSNSQGALVRDPAFKIKSLDCFTVDIPLPRPAHN 75

Query: 137 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGEHQS 196
           EP+DI L+IV+EDEH+LVVNKPA MVVHPA GN   TLVNA+L HC+        G+   
Sbjct: 76  EPQDIPLEIVFEDEHLLVVNKPAGMVVHPAAGNYDNTLVNALLYHCA--------GKLSG 135

Query: 197 DSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSI 256
              V+                  RPGIVHR+DK TSGLLVVAK + +HA L+AQF  HSI
Sbjct: 136 IGGVA------------------RPGIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHSI 195

Query: 257 ERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHAKYAASRYNVMEILAQ 316
            R Y ++  G  PS  G ++ P+ R   NR +M  +S    +   K A + Y ++  L  
Sbjct: 196 NRRYRAIVDG-HPSLQGHVDAPLARSSVNRQKMAIVS----DGRGKRAVTHYRMITPLK- 255

Query: 317 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLIQ 376
             +SL+E RLETGRTHQ+R H   +G  LLGD VYG +K    +LL++ A          
Sbjct: 256 -NASLIECRLETGRTHQVRVHMSSIGHSLLGDPVYGRSKKAHHALLQSLA---------- 311

Query: 377 LVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 419
                +R  LHA  LGF+HP +GK + F    P D   +   L
Sbjct: 316 ----FQRQALHAAHLGFIHPISGKQVDFDAEMPQDMQLLFKML 311

BLAST of PI0005671 vs. ExPASy Swiss-Prot
Match: O50310 (Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=Cpar_0723 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.3e-51
Identity = 128/348 (36.78%), Postives = 193/348 (55.46%), Query Frame = 0

Query: 76  VRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDMINCTISELQPLRA 135
           +R+D +++ ++  ++R +VQ++I  G V VNG+ + K ++ VK+ D I+ T         
Sbjct: 12  MRIDRYLTQQVENMTRNKVQAAIEEGRVLVNGKPV-KSNYRVKSCDHIHLTFLRPPAPEL 71

Query: 136 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGEHQS 195
            PEDI + I+YED+ ++V++K A MVVHPA GN TGTL NAIL+H               
Sbjct: 72  APEDIPITILYEDDDLMVIDKEAGMVVHPAFGNWTGTLANAILHHLG------------K 131

Query: 196 DSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSI 255
           D+D  D             +  +RPGIVHRLDK TSGL+++AK+  +   L+ QF    +
Sbjct: 132 DADELD-------------KTEMRPGIVHRLDKDTSGLIIIAKNPTALHKLARQFANRQV 191

Query: 256 ERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHAKYAASRYNVMEILAQ 315
           E+VY ++  GVP + +G I+  I R   NR  M   +        K+A + Y V+E L  
Sbjct: 192 EKVYKAIVWGVPKAESGTIKTNIGRSHKNRKVMA--NFPYEGAEGKHAITDYQVVEDL-- 251

Query: 316 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLIQ 375
           G  SL++  L TGRTHQIR H +++G  +LGDE YGG     +   ++   +     L++
Sbjct: 252 GYFSLMDVTLHTGRTHQIRVHLQHLGHSILGDETYGGATPRTLPFSKSEPFTR---NLLE 311

Query: 376 LVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLREIGT 424
           L+S   R  LHA TL F  P TG+ + FS P P D   +L ++R + T
Sbjct: 312 LMS---RQALHAQTLRFRQPTTGEPLSFSAPLPEDMELVLEKIRTVMT 323

BLAST of PI0005671 vs. ExPASy TrEMBL
Match: A0A1S3BCE4 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 1.1e-238
Identity = 417/426 (97.89%), Postives = 422/426 (99.06%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRI SNVLRFSANFS+RCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AG RLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           D+INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMV+TSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTA  GTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           K+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LRTRASSNCHGQL+QLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKL 427
           IGTKKL
Sbjct: 421 IGTKKL 426

BLAST of PI0005671 vs. ExPASy TrEMBL
Match: A0A1S3BD56 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.8e-238
Identity = 417/429 (97.20%), Postives = 423/429 (98.60%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRI SNVLRFSANFS+RCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AG RLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           D+INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMV+TSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTA  GTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           K+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LRTRASSNCHGQL+QLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKLGAS 430
           IGTKK+  S
Sbjct: 421 IGTKKVTKS 429

BLAST of PI0005671 vs. ExPASy TrEMBL
Match: A0A0A0LKD7 (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.1e-233
Identity = 412/438 (94.06%), Postives = 420/438 (95.89%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRC-SDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFSTRC SDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAG RLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD+INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTAI GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420
           LLRTRASSNCHGQL+QLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTKKLGASSIWQVDSS 438
           EIGT+K      WQV  S
Sbjct: 421 EIGTQK------WQVTKS 432

BLAST of PI0005671 vs. ExPASy TrEMBL
Match: A0A5A7VHA3 (Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001610 PE=3 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 4.4e-216
Identity = 382/391 (97.70%), Postives = 387/391 (98.98%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRI SNVLRFSANFS+RCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AG RLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           D+INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMV+TSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTA  GTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           K+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLIQLVSSLERPCLHALTLG 392
           LRTRASSNCHGQL+QLVSSLERPCLHALTLG
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 391

BLAST of PI0005671 vs. ExPASy TrEMBL
Match: A0A6J1CMS3 (Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.8e-209
Identity = 371/430 (86.28%), Postives = 394/430 (91.63%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQSERIANY 60
           MISVCS R IQA+P SI  FFPRNR RITSNV R + N STRCSDYDE  EA SERI NY
Sbjct: 1   MISVCSVRAIQAAP-SIQLFFPRNRGRITSNVHRIATNHSTRCSDYDECGEAHSERITNY 60

Query: 61  AGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AG+RLEE VD+N GK+RLDSWIS RINGISRARVQSSIRAGLV+VNGRVIDKVSHN+KAG
Sbjct: 61  AGMRLEEIVDVNSGKIRLDSWISCRINGISRARVQSSIRAGLVTVNGRVIDKVSHNIKAG 120

Query: 121 DMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DMINCTISELQPLRA+PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DMINCTISELQPLRAKPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVATSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           CSLPMVATS GE  SD D+SDDEFSS E+  SR SEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 CSLPMVATSNGE-LSDMDISDDEFSSNEILSSRASEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRM+A+  T  NPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMSAVLRTNKNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRY V+EILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMA+S
Sbjct: 301 AKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMALS 360

Query: 361 LLRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420
           LLRTR SS+CH QL+QLVS+LERPCLHALTLGF+HP+TG+NIRFSCPPPTDFA+I SQL 
Sbjct: 361 LLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTGENIRFSCPPPTDFADIYSQLC 420

Query: 421 EIGTKKLGAS 430
           EIGTKK+  S
Sbjct: 421 EIGTKKVTES 428

BLAST of PI0005671 vs. NCBI nr
Match: XP_008445318.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo] >XP_008445319.1 PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 835.5 bits (2157), Expect = 2.2e-238
Identity = 417/426 (97.89%), Postives = 422/426 (99.06%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRI SNVLRFSANFS+RCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AG RLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           D+INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMV+TSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTA  GTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           K+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LRTRASSNCHGQL+QLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKL 427
           IGTKKL
Sbjct: 421 IGTKKL 426

BLAST of PI0005671 vs. NCBI nr
Match: XP_008445320.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 834.7 bits (2155), Expect = 3.8e-238
Identity = 417/429 (97.20%), Postives = 423/429 (98.60%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRI SNVLRFSANFS+RCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AG RLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           D+INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMV+TSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTA  GTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           K+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LRTRASSNCHGQL+QLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKLGAS 430
           IGTKK+  S
Sbjct: 421 IGTKKVTKS 429

BLAST of PI0005671 vs. NCBI nr
Match: KGN62268.1 (hypothetical protein Csa_018737 [Cucumis sativus])

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-232
Identity = 412/438 (94.06%), Postives = 420/438 (95.89%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRC-SDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFSTRC SDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAG RLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD+INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTAI GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420
           LLRTRASSNCHGQL+QLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTKKLGASSIWQVDSS 438
           EIGT+K      WQV  S
Sbjct: 421 EIGTQK------WQVTKS 432

BLAST of PI0005671 vs. NCBI nr
Match: XP_031736623.1 (RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 814.7 bits (2103), Expect = 4.0e-232
Identity = 408/426 (95.77%), Postives = 416/426 (97.65%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRC-SDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFSTRC SDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAG RLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD+INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTAI GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420
           LLRTRASSNCHGQL+QLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTKK 426
           EIGT+K
Sbjct: 421 EIGTQK 426

BLAST of PI0005671 vs. NCBI nr
Match: XP_038886062.1 (RNA pseudouridine synthase 2, chloroplastic isoform X4 [Benincasa hispida])

HSP 1 Score: 785.8 bits (2028), Expect = 2.0e-223
Identity = 389/426 (91.31%), Postives = 406/426 (95.31%), Query Frame = 0

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRITSNVLRFSANFSTRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSI  FFPRNRRRIT +VLRFS N STR SDYDEY EAQSER AN+
Sbjct: 1   MISVCSARTIQASPSSIQLFFPRNRRRITPDVLRFSVNLSTRSSDYDEYGEAQSERRANF 60

Query: 61  AGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGIRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGR+IDKVSHNVKAG
Sbjct: 61  AGIRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRIIDKVSHNVKAG 120

Query: 121 DMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DMINCTISELQPLRAEPEDIDLDIVYED HVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DMINCTISELQPLRAEPEDIDLDIVYEDAHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVATS GE  SD D+SDDEFSS+E+FSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVATSKGELPSDMDISDDEFSSVEIFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAISGTKNNPHA 300
           HSHAHLSAQFK HSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA+SGT NNPHA
Sbjct: 241 HSHAHLSAQFKLHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMTAVSGTNNNPHA 300

Query: 301 KYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           K+AASRY VMEILAQGGSSLVEWRLETGRTHQIRAHAK+MGIPLLGD+VYGGTKSMAMSL
Sbjct: 301 KHAASRYKVMEILAQGGSSLVEWRLETGRTHQIRAHAKHMGIPLLGDDVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420
           LR+R SS+CH QL+Q +SSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE
Sbjct: 361 LRSRTSSSCHSQLMQHISSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLRE 420

Query: 421 IGTKKL 427
           IGTKK+
Sbjct: 421 IGTKKV 426

BLAST of PI0005671 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.4e-145
Identity = 277/431 (64.27%), Postives = 327/431 (75.87%), Query Frame = 0

Query: 3   SVCSARTIQ--ASPS---SIHF-FFPRNRRRITSNVLRFSANFS--TRCSDYDEYSEAQS 62
           S+ +A++++  +SPS   SI F FFP+      SN +R S+       CS     +E   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK-----VSNFVRASSGIPNLVACSP----TEIII 70

Query: 63  ERIANYAGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVS 122
            R+ N AG+R+EE VD   GK+RLDSWISSRING+SRARVQSSIR GLV+VNGRV+DKVS
Sbjct: 71  PRV-NNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVS 130

Query: 123 HNVKAGDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLV 182
           HNVK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLV
Sbjct: 131 HNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLV 190

Query: 183 NAILNHCSLPMVATSTGEHQSDSD---VSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTS 242
           N IL+HCSLP V  S  E   DSD    SDDE  +    S    A +RPGIVHRLDKGT+
Sbjct: 191 NGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTT 250

Query: 243 GLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAI 302
           GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIEIPI RD +NRIRM AI
Sbjct: 251 GLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAI 310

Query: 303 SGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYG 362
            G      A++AASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYG
Sbjct: 311 PGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYG 370

Query: 363 GTKSMAMSLLRTRASSNCHGQLIQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF 422
           GTKSMA+SLL+ R S +   ++I+L+S ++RPCLHA+ LGF HP TG+ ++FSCPPP+D 
Sbjct: 371 GTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPSDL 429

BLAST of PI0005671 vs. TAIR 10
Match: AT1G76050.1 (Pseudouridine synthase family protein )

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-94
Identity = 199/315 (63.17%), Postives = 231/315 (73.33%), Query Frame = 0

Query: 3   SVCSARTIQ--ASPS---SIHF-FFPRNRRRITSNVLRFSANFS--TRCSDYDEYSEAQS 62
           S+ +A++++  +SPS   SI F FFP+      SN +R S+       CS     +E   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK-----VSNFVRASSGIPNLVACSP----TEIII 70

Query: 63  ERIANYAGIRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVS 122
            R+ N AG+R+EE VD   GK+RLDSWISSRING+SRARVQSSIR GLV+VNGRV+DKVS
Sbjct: 71  PRV-NNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVS 130

Query: 123 HNVKAGDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLV 182
           HNVK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLV
Sbjct: 131 HNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLV 190

Query: 183 NAILNHCSLPMVATSTGEHQSDSD---VSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTS 242
           N IL+HCSLP V  S  E   DSD    SDDE  +    S    A +RPGIVHRLDKGT+
Sbjct: 191 NGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTT 250

Query: 243 GLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAI 302
           GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIEIPI RD +NRIRM AI
Sbjct: 251 GLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAI 310

Query: 303 SGTKNNPHAKYAASR 307
            G      A++AASR
Sbjct: 311 PGGVRGGRARHAASR 313

BLAST of PI0005671 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 76.6 bits (187), Expect = 5.6e-14
Identity = 70/272 (25.74%), Postives = 117/272 (43.01%), Query Frame = 0

Query: 98  IRAGLVSVNGRVIDKVSHNVKAGDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKP 157
           I+ G + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKP 115

Query: 158 AHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAP 217
           + + V P  G      V   L  C                      F   + +    E+P
Sbjct: 116 SGLQVLPG-GLFQQRTVLTQLQWC----------------------FGKNDSYIGSRESP 175

Query: 218 IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYI 277
             P  VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y 
Sbjct: 176 -HPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 235

Query: 278 SLTCGVPPSTAGRIEIPI--CRDPNNRIRMTAISGTKNNPHAKYAASRYNVMEILAQGGS 337
           +L  G+       I+ PI   R P     +   S     P  K A S+  V+E   +   
Sbjct: 236 ALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVAS-----PEGKPAFSKVFVLERDREKNC 295

Query: 338 SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 351
           SLV+  +++GR HQIR H  YMG PL+GD +Y
Sbjct: 296 SLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

BLAST of PI0005671 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 68.9 bits (167), Expect = 1.2e-11
Identity = 66/273 (24.18%), Postives = 116/273 (42.49%), Query Frame = 0

Query: 103 VSVNGRVIDKVSHNVKAGDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVV 162
           + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP+ + V
Sbjct: 91  IQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQV 150

Query: 163 HPAPGNATGTLVNAILNHCSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGI 222
            P  G      V   L  C                      F   + +    E+P  P  
Sbjct: 151 LPG-GLFQQRTVLTQLQWC----------------------FGKNDSYIGSRESP-HPVP 210

Query: 223 VHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYISLTCG 282
           VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y +L  G
Sbjct: 211 VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG 270

Query: 283 VPPSTAGRIEIPI--CRDPNNRIRMTAISGTKNNPHAKYAASRYNVMEILAQGGSSLVEW 342
           +       I+ PI   R P     +   S     P  K A S+  V+E   +   SLV+ 
Sbjct: 271 IVEEDEVVIKQPIGVVRYPGVAQGLYVAS-----PEGKPAFSKVFVLERDREKNCSLVKV 330

Query: 343 RLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSM 357
            +++GR HQIR H  YMG PL+   ++ G++ +
Sbjct: 331 EIQSGRPHQIRIHLAYMGHPLVEMTMFTGSECL 331

BLAST of PI0005671 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 68.6 bits (166), Expect = 1.5e-11
Identity = 67/266 (25.19%), Postives = 112/266 (42.11%), Query Frame = 0

Query: 98  IRAGLVSVNGRVIDKVSHNVKAGDMINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKP 157
           I+ G + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKP 115

Query: 158 AHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGEHQSDSDVSDDEFSSIEVFSRVSEAP 217
           + + V P  G      V   L  C                      F   + +    E+P
Sbjct: 116 SGLQVLPG-GLFQQRTVLTQLQWC----------------------FGKNDSYIGSRESP 175

Query: 218 IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYI 277
             P  VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y 
Sbjct: 176 -HPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 235

Query: 278 SLTCGVPPSTAGRIEIPI--CRDPNNRIRMTAISGTKNNPHAKYAASRYNVMEILAQGGS 337
           +L  G+       I+ PI   R P     +   S     P  K A S+  V+E   +   
Sbjct: 236 ALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVAS-----PEGKPAFSKVFVLERDREKNC 289

Query: 338 SLVEWRLETGRTHQIRAHAKYMGIPL 345
           SLV+  +++GR HQIR H  YMG PL
Sbjct: 296 SLVKVEIQSGRPHQIRIHLAYMGHPL 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3ECD01.9e-14464.27RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q5Z8P21.3e-13759.63RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
Q454803.7e-5538.73Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168... [more]
P505131.4e-5439.94Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mob... [more]
O503104.3e-5136.78Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (st... [more]
Match NameE-valueIdentityDescription
A0A1S3BCE41.1e-23897.89Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A1S3BD561.8e-23897.20Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A0A0LKD75.1e-23394.06Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1[more]
A0A5A7VHA34.4e-21697.70Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A6J1CMS31.8e-20986.28Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008445318.12.2e-23897.89PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo]... [more]
XP_008445320.13.8e-23897.20PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo][more]
KGN62268.11.1e-23294.06hypothetical protein Csa_018737 [Cucumis sativus][more]
XP_031736623.14.0e-23295.77RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus][more]
XP_038886062.12.0e-22391.31RNA pseudouridine synthase 2, chloroplastic isoform X4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G76050.21.4e-14564.27Pseudouridine synthase family protein [more]
AT1G76050.11.1e-9463.17Pseudouridine synthase family protein [more]
AT3G52260.25.6e-1425.74Pseudouridine synthase family protein [more]
AT3G52260.31.2e-1124.18Pseudouridine synthase family protein [more]
AT3G52260.11.5e-1125.19Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002942RNA-binding S4 domainSMARTSM00363s4_6coord: 76..138
e-value: 1.3E-6
score: 37.9
IPR002942RNA-binding S4 domainPFAMPF01479S4coord: 76..123
e-value: 2.5E-9
score: 36.7
IPR002942RNA-binding S4 domainCDDcd00165S4coord: 76..155
e-value: 5.379E-14
score: 64.5782
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 150..337
e-value: 1.0E-29
score: 103.7
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 151..392
e-value: 1.00676E-63
score: 202.182
IPR006225Pseudouridine synthase, RluC/RluDTIGRFAMTIGR00005TIGR00005coord: 76..419
e-value: 2.4E-75
score: 251.5
IPR036986RNA-binding S4 domain superfamilyGENE3D3.10.290.10coord: 61..131
e-value: 4.3E-16
score: 60.1
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 133..407
e-value: 2.2E-67
score: 228.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 432..454
NoneNo IPR availablePANTHERPTHR21600:SF57RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Dcoord: 74..420
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 74..420
NoneNo IPR availablePROSITEPS50889S4coord: 76..149
score: 12.257083
NoneNo IPR availableSUPERFAMILY55174Alpha-L RNA-binding motifcoord: 75..163
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 223..237
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 136..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005671.1PI0005671.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding