PI0005574 (gene) Melon (PI 482460) v1

BLAST of PI0005574 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.5e-54
Identity = 125/323 (38.70%), Postives = 175/323 (54.18%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNFIS-IIFKANYSSYVTDFPGH------------------------F 78
           L+F     DTGNNN+ S  +FKAN+  Y  D PGH                        F
Sbjct: 35  LIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLNIKEF 94

Query: 79  VPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGL 138
           VPPFL P +SD D+  GV FASAG+G+D+ T+  S  IPV +Q  MFKNY  RL+GIVG 
Sbjct: 95  VPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKGIVGD 154

Query: 139 NESRKILDSALVVISAGTNDY---------------NISGYQDFLQNRLQSLIK------ 198
            ++ +I+++ALVVISAG ND+                I GYQDF+  RL   ++      
Sbjct: 155 KKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVRELYSLG 214

Query: 199 -----VIGLPPVGCLPIHES--------IAFQNLKIENVWKNKTQIPK-PTIRSFFLEAQ 258
                V GLPP+GCLPI  +        I  +    +++  N+  + K P I++    ++
Sbjct: 215 CRNILVGGLPPMGCLPIQLTAKLRTILGICVEQENKDSILYNQKLVKKLPEIQASLPGSK 274

Query: 259 -LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDN 281
            LYA++Y P++DM+ NP  YGF+    GCCG G +E   LC + + T C N S  +FWD+
Sbjct: 275 FLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTSLSKT-CPNHSDHLFWDS 334

BLAST of PI0005574 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 210.3 bits (534), Expect = 2.8e-53
Identity = 120/315 (38.10%), Postives = 172/315 (54.60%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNF-ISIIFKANYSSYVTDFPG------------------------HF 78
           L+F     DTGNNN+ +  IF+A +  Y  D P                          F
Sbjct: 37  LIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLNIKEF 96

Query: 79  VPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGL 138
           +PPFL P LSD D+  GV FASAG+G+D+LT+  +  I V +Q +MFK+Y  RL+GIVG 
Sbjct: 97  IPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKGIVGD 156

Query: 139 NESRKILDSALVVISAGTNDY---------------NISGYQDFLQNRLQSLIK------ 198
            ++ +I+++A VV+SAG ND+                ISGYQDF+  RL++ ++      
Sbjct: 157 KKAMEIINNAFVVVSAGPNDFILNYYDIPSRRLEYPFISGYQDFILKRLENFVRELYSLG 216

Query: 199 -----VIGLPPVGCLPIHESIAFQNL---------KIENVWKNKTQIPKPTIRSFFLEAQ 258
                V GLPP+GCLPIH +  F+N+         K   ++  K Q   P I +    ++
Sbjct: 217 VRNVLVGGLPPMGCLPIHMTAKFRNIFRFCLEHHNKDSVLYNEKLQKLLPQIEASLPGSK 276

Query: 259 -LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDN 273
            LYAD+Y P+++M+ NP  YGF+    GCCG G +E   +C N  S +C+N S+FMF+D+
Sbjct: 277 FLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSFMC-NVFSPVCQNRSEFMFFDS 336

BLAST of PI0005574 vs. ExPASy Swiss-Prot
Match: Q9LMJ3 (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 4.8e-53
Identity = 121/317 (38.17%), Postives = 166/317 (52.37%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNFISIIFKANYSSYVTDFPGH------------------------FV 78
           L+F     DTGNNN+I    +AN+  Y  +FPGH                         V
Sbjct: 39  LVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGIKDTV 98

Query: 79  PPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLN 138
           PPFLDP LSD D+  GV FASAGSG+D LT   ++ + V KQ DM ++Y +RL  IVG  
Sbjct: 99  PPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQIVGDE 158

Query: 139 ESRKILDSALVVISAGTNDYN--------------ISGYQDFL----QNRLQSL------ 198
           ++  I+  ALV++S+GTND+N              + GYQ F+     N +Q L      
Sbjct: 159 KAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYDIGCR 218

Query: 199 -IKVIGLPPVGCLPIHESIAFQ---NLKIENVWKNKTQIPKPTIRSFFLEAQ-------- 258
            I V+GLPPVGCLPI  ++A Q     +  +   + +Q     +++   E Q        
Sbjct: 219 KIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLTGSVI 278

Query: 259 LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDNI 276
            Y DIY  L DM  NP  YG +    GCCG G +E   LCN  T  IC NP++++FWD+I
Sbjct: 279 FYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALT-RICPNPNQYLFWDDI 338

BLAST of PI0005574 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 4.8e-53
Identity = 123/311 (39.55%), Postives = 168/311 (54.02%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNFIS-IIFKANYSSYVTDFPGH------------------------F 78
           L+F     DTGNNN+ S  IFKA +  Y  D PGH                         
Sbjct: 36  LIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLNIKEL 95

Query: 79  VPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGL 138
           VPPFL P +S  D+  GVSFASAG+G+D+ ++  S  IPV +Q  MFKNY  RL+GIVG 
Sbjct: 96  VPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKGIVGD 155

Query: 139 NESRKILDSALVVISAGTNDY---------------NISGYQDFLQNRLQSLIK------ 198
            ++ +I+++ALVVISAG ND+                I GYQ+F+  RL   ++      
Sbjct: 156 KKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVRELYSLG 215

Query: 199 -----VIGLPPVGCLPIHESIAFQNL--------KIENVWKNKTQIPK-PTIRSFFLEAQ 258
                V GLPP+GCLPI  +   +N+          ++V  N+  + K P I++    + 
Sbjct: 216 CRNIVVGGLPPMGCLPIQMTAKMRNILRFCVEQENKDSVLYNQKLVKKLPEIQASLPGSN 275

Query: 259 -LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDN 269
            LYA++Y PL+DM+ NP  YGF+    GCCG G +E   +CN  T T C N S  +FWD+
Sbjct: 276 FLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLTKT-CPNHSDHLFWDS 335

BLAST of PI0005574 vs. ExPASy Swiss-Prot
Match: Q9SIQ3 (GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 8.2e-53
Identity = 118/311 (37.94%), Postives = 171/311 (54.98%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNF-ISIIFKANYSSYVTDFPG------------------------HF 78
           L+F     DTGNNN+ +  IF+A +  Y  D P                          F
Sbjct: 37  LIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLNIKEF 96

Query: 79  VPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGL 138
           +PPFL P LSD D+  GV FASAG+G+D+LT+  +  I V +Q +MFK+Y  RL+GIVG 
Sbjct: 97  IPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKGIVGD 156

Query: 139 NESRKILDSALVVISAGTNDY---------------NISGYQDFLQNRLQSLIK------ 198
            ++ +I+++A VV+SAG ND+                ISGYQDF+  RL++ ++      
Sbjct: 157 KKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVRELYSLG 216

Query: 199 -----VIGLPPVGCLPIHESIAFQNL---------KIENVWKNKTQIPKPTIRSFFLEAQ 258
                V GLPP+GCLPIH +  F+N+         K   ++  K Q   P I +    ++
Sbjct: 217 VRNVLVGGLPPMGCLPIHMTAKFRNIFRFCLEHHNKDSVLYNEKLQNLLPQIEASLPGSK 276

Query: 259 -LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDN 269
            LYAD+Y P+++M+ NP  YGF+    GCCG G +E   +C N  S +C+N S+F+F+D+
Sbjct: 277 FLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSFMC-NVFSPVCQNRSEFLFFDS 336

BLAST of PI0005574 vs. ExPASy TrEMBL
Match: A0A5A7UVN0 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852G00270 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.4e-111
Identity = 233/371 (62.80%), Postives = 248/371 (66.85%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLLFFSKP----------------------FDTGNNNFISIIF 60
           M R TYHIAALS LH+IWLLF SKP                       DTGNNNFIS IF
Sbjct: 1   MARATYHIAALS-LHMIWLLFLSKPCTALEPKITRSFPSILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSFA 120
           KANYS Y  DFPGH                         VPPFLD KLSDD+VK GVSFA
Sbjct: 61  KANYSPYGADFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDTKLSDDNVKTGVSFA 120

Query: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND- 180
           SAGSGFDELTASVSNVIPVMKQIDMFKNY +RLQGIVG++ESRKIL+SALV+ISAGTND 
Sbjct: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYIRRLQGIVGVDESRKILNSALVIISAGTNDV 180

Query: 181 -------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESIA 240
                        YNISGYQDFLQ+RLQSLIK           V GLPPVGCLPI ESIA
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQDRLQSLIKEIYQLGCRTMVVAGLPPVGCLPIQESIA 240

Query: 241 FQ------------------NLKIENVWKN-KTQIPKPTIRSFFLEAQLYADIYTPLIDM 282
           FQ                  N K+ ++  N + Q+P  TI        LYADIYTPLIDM
Sbjct: 241 FQNPLDRKCLEEQNSDSKAYNQKLAHLLSNLQPQLPGSTI--------LYADIYTPLIDM 300

BLAST of PI0005574 vs. ExPASy TrEMBL
Match: A0A1S3CKL4 (GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501878 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.4e-111
Identity = 233/371 (62.80%), Postives = 248/371 (66.85%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLLFFSKP----------------------FDTGNNNFISIIF 60
           M R TYHIAALS LH+IWLLF SKP                       DTGNNNFIS IF
Sbjct: 1   MARATYHIAALS-LHMIWLLFLSKPCTALEPKITRSFPSILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSFA 120
           KANYS Y  DFPGH                         VPPFLD KLSDD+VK GVSFA
Sbjct: 61  KANYSPYGADFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDTKLSDDNVKTGVSFA 120

Query: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND- 180
           SAGSGFDELTASVSNVIPVMKQIDMFKNY +RLQGIVG++ESRKIL+SALV+ISAGTND 
Sbjct: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYIRRLQGIVGVDESRKILNSALVIISAGTNDV 180

Query: 181 -------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESIA 240
                        YNISGYQDFLQ+RLQSLIK           V GLPPVGCLPI ESIA
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQDRLQSLIKEIYQLGCRTMVVAGLPPVGCLPIQESIA 240

Query: 241 FQ------------------NLKIENVWKN-KTQIPKPTIRSFFLEAQLYADIYTPLIDM 282
           FQ                  N K+ ++  N + Q+P  TI        LYADIYTPLIDM
Sbjct: 241 FQNPLDRKCLEEQNSDSKAYNQKLAHLLSNLQPQLPGSTI--------LYADIYTPLIDM 300

BLAST of PI0005574 vs. ExPASy TrEMBL
Match: A0A0A0KC28 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G180990 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.9e-100
Identity = 212/347 (61.10%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLLFFSKP----------------------FDTGNNNFISIIF 60
           M + TYHIA+LS LH+IWLLF SKP                       DTGNNNFIS IF
Sbjct: 1   MAKATYHIASLS-LHMIWLLFLSKPCTALEPKITRSFLSILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYSSYVTDFPGHFVPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQID 120
           KANYS Y TDFPGH +             +  V FASAGSGFDELTASVSNVI VMKQID
Sbjct: 61  KANYSPYGTDFPGHILN-------CGQRCENRVGFASAGSGFDELTASVSNVISVMKQID 120

Query: 121 MFKNYTQRLQGIVGLNESRKILDSALVVISAGTND--------------YNISGYQDFLQ 180
           MFKNYT+RLQGIVG++ESRKIL+SALVVISAGTND              YNISGYQDF+Q
Sbjct: 121 MFKNYTRRLQGIVGVDESRKILNSALVVISAGTNDVNINFYDLPIRQLQYNISGYQDFVQ 180

Query: 181 NRLQSLIK-----------VIGLPPVGCLPIHESIAFQ------------------NLKI 240
           NRLQSLIK           V GLPPVGCLPI ESIAFQ                  N K+
Sbjct: 181 NRLQSLIKEIYQLGCRTIVVAGLPPVGCLPIQESIAFQKPQDRKCLEEQNSDFKAYNQKL 240

Query: 241 ENVWKN-KTQIPKPTIRSFFLEAQLYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEA 282
            ++  N + Q+P  TI        LY DIYTPLIDMVNNPHNYGFEHVNVGCCG GM EA
Sbjct: 241 AHLLSNLQPQLPGSTI--------LYGDIYTPLIDMVNNPHNYGFEHVNVGCCGTGMAEA 300

BLAST of PI0005574 vs. ExPASy TrEMBL
Match: A0A5A7VF47 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001150 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 7.1e-92
Identity = 198/363 (54.55%), Postives = 227/363 (62.53%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLLFFSKP----------------------FDTGNNNFISIIF 60
           MTR+   IA LS LH IWLL  +KP                       DTGNNNFI  IF
Sbjct: 1   MTRVNCLIATLS-LHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIF 60

Query: 61  KANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSFA 120
           K NYS Y  +FPGH                         VPPFLDPKLS+DD+K GVSFA
Sbjct: 61  KGNYSPYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFA 120

Query: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND- 180
           SAG+GFD+LTA++S VIPVMKQID FKNY QRLQG+VG+ ES++I+ +ALVVISAGTND 
Sbjct: 121 SAGTGFDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDL 180

Query: 181 -------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESIA 240
                        YNISGYQDFLQNRL+SLIK           V GLPPVGCLPI E+IA
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIA 240

Query: 241 FQN------LKIEN----VWKNKTQIPKPTIRSFFLEAQ-LYADIYTPLIDMVNNPHNYG 282
           F+N      L+ +N     +  K       ++     ++ LYADIYTPLIDM+NNP NYG
Sbjct: 241 FENPLKRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKILYADIYTPLIDMLNNPQNYG 300

BLAST of PI0005574 vs. ExPASy TrEMBL
Match: A0A1S4E4N0 (GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501132 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 7.1e-92
Identity = 198/363 (54.55%), Postives = 227/363 (62.53%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLLFFSKP----------------------FDTGNNNFISIIF 60
           MTR+   IA LS LH IWLL  +KP                       DTGNNNFI  IF
Sbjct: 1   MTRVNCLIATLS-LHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIF 60

Query: 61  KANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSFA 120
           K NYS Y  +FPGH                         VPPFLDPKLS+DD+K GVSFA
Sbjct: 61  KGNYSPYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFA 120

Query: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND- 180
           SAG+GFD+LTA++S VIPVMKQID FKNY QRLQG+VG+ ES++I+ +ALVVISAGTND 
Sbjct: 121 SAGTGFDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDL 180

Query: 181 -------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESIA 240
                        YNISGYQDFLQNRL+SLIK           V GLPPVGCLPI E+IA
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIA 240

Query: 241 FQN------LKIEN----VWKNKTQIPKPTIRSFFLEAQ-LYADIYTPLIDMVNNPHNYG 282
           F+N      L+ +N     +  K       ++     ++ LYADIYTPLIDM+NNP NYG
Sbjct: 241 FENPLKRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKILYADIYTPLIDMLNNPQNYG 300

BLAST of PI0005574 vs. NCBI nr
Match: XP_008463830.1 (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059942.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07587.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 412.5 bits (1059), Expect = 2.9e-111
Identity = 233/371 (62.80%), Postives = 248/371 (66.85%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLLFFSKP----------------------FDTGNNNFISIIF 60
           M R TYHIAALS LH+IWLLF SKP                       DTGNNNFIS IF
Sbjct: 1   MARATYHIAALS-LHMIWLLFLSKPCTALEPKITRSFPSILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSFA 120
           KANYS Y  DFPGH                         VPPFLD KLSDD+VK GVSFA
Sbjct: 61  KANYSPYGADFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDTKLSDDNVKTGVSFA 120

Query: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND- 180
           SAGSGFDELTASVSNVIPVMKQIDMFKNY +RLQGIVG++ESRKIL+SALV+ISAGTND 
Sbjct: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYIRRLQGIVGVDESRKILNSALVIISAGTNDV 180

Query: 181 -------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESIA 240
                        YNISGYQDFLQ+RLQSLIK           V GLPPVGCLPI ESIA
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQDRLQSLIKEIYQLGCRTMVVAGLPPVGCLPIQESIA 240

Query: 241 FQ------------------NLKIENVWKN-KTQIPKPTIRSFFLEAQLYADIYTPLIDM 282
           FQ                  N K+ ++  N + Q+P  TI        LYADIYTPLIDM
Sbjct: 241 FQNPLDRKCLEEQNSDSKAYNQKLAHLLSNLQPQLPGSTI--------LYADIYTPLIDM 300

BLAST of PI0005574 vs. NCBI nr
Match: XP_016902930.1 (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0066338.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK00940.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 347.1 bits (889), Expect = 1.5e-91
Identity = 198/363 (54.55%), Postives = 227/363 (62.53%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLLFFSKP----------------------FDTGNNNFISIIF 60
           MTR+   IA LS LH IWLL  +KP                       DTGNNNFI  IF
Sbjct: 1   MTRVNCLIATLS-LHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIF 60

Query: 61  KANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSFA 120
           K NYS Y  +FPGH                         VPPFLDPKLS+DD+K GVSFA
Sbjct: 61  KGNYSPYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFA 120

Query: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND- 180
           SAG+GFD+LTA++S VIPVMKQID FKNY QRLQG+VG+ ES++I+ +ALVVISAGTND 
Sbjct: 121 SAGTGFDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDL 180

Query: 181 -------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESIA 240
                        YNISGYQDFLQNRL+SLIK           V GLPPVGCLPI E+IA
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIA 240

Query: 241 FQN------LKIEN----VWKNKTQIPKPTIRSFFLEAQ-LYADIYTPLIDMVNNPHNYG 282
           F+N      L+ +N     +  K       ++     ++ LYADIYTPLIDM+NNP NYG
Sbjct: 241 FENPLKRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKILYADIYTPLIDMLNNPQNYG 300

BLAST of PI0005574 vs. NCBI nr
Match: XP_004151059.1 (GDSL esterase/lipase At2g30310 [Cucumis sativus] >KGN63338.1 hypothetical protein Csa_022358 [Cucumis sativus])

HSP 1 Score: 344.7 bits (883), Expect = 7.3e-91
Identity = 196/364 (53.85%), Postives = 223/364 (61.26%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLL-----------------------FFSKPFDTGNNNFISII 60
           M R+   IAALS LH IWLL                       F     DTGNNNFI  I
Sbjct: 1   MARVNCLIAALS-LHTIWLLVVLTKPCSSLEPKTTPSFPAILIFGDSTVDTGNNNFIPTI 60

Query: 61  FKANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSF 120
           FK NYS Y  +FPGH                         VPPFLDPKLS+DD+K GVSF
Sbjct: 61  FKGNYSPYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSF 120

Query: 121 ASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND 180
           ASAG+GFD+LTA++S VIPVMKQID FKNY QRLQG+VG++ES++I+++ALVVISAGTND
Sbjct: 121 ASAGTGFDDLTAAISKVIPVMKQIDHFKNYIQRLQGVVGVDESKRIINNALVVISAGTND 180

Query: 181 --------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESI 240
                         YNISGYQDFLQNRLQSLIK           V GLPPVGCLPI E+I
Sbjct: 181 LNINFYDLPTRQLQYNISGYQDFLQNRLQSLIKEIYQLGCRNIVVAGLPPVGCLPIQETI 240

Query: 241 AFQNLKIENVWKNKTQ---IPKPTIRSFFLEAQ--------LYADIYTPLIDMVNNPHNY 282
           AF+N    N  K++          +       Q        LYADIYTPLIDM+NNP  Y
Sbjct: 241 AFENPLKRNCLKDQNSDSVAYNQKLSKLLTNLQPQLAGSKILYADIYTPLIDMLNNPQKY 300

BLAST of PI0005574 vs. NCBI nr
Match: XP_004143225.1 (GDSL esterase/lipase At2g30310 [Cucumis sativus])

HSP 1 Score: 344.0 bits (881), Expect = 1.2e-90
Identity = 195/364 (53.57%), Postives = 225/364 (61.81%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIWLLFFSKP----------------------FDTGNNNFISIIF 60
           M R  Y IAALS+ H+IWLLF SKP                       DTGNNNFI  IF
Sbjct: 1   MARANYLIAALSI-HIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIF 60

Query: 61  KANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSFA 120
           KANY  Y  DFPGH                         VPPFLDP+LSDDDVK GVSFA
Sbjct: 61  KANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFA 120

Query: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND- 180
           SAG+G D+LTA++S VIP MKQIDMFKNY QRLQ IVG++ES++I+ SAL VIS GTND 
Sbjct: 121 SAGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDL 180

Query: 181 -------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESIA 240
                        YNISGYQ+FLQNRLQSLIK           V GLPP+GCLPI E+I+
Sbjct: 181 TFNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 240

Query: 241 -----------FQNLKIENVWKNKTQIPKPTIRSFFLEAQ-LYADIYTPLIDMVNNPHNY 282
                      +QN K    +  K      +++     +Q LYADIYTPL+DM+NNP  Y
Sbjct: 241 SPIPLNRRCLEYQN-KDAEAYNQKLSKLLGSLQPQLPGSQILYADIYTPLMDMINNPQKY 300

BLAST of PI0005574 vs. NCBI nr
Match: XP_022947820.1 (GDSL esterase/lipase At2g30310-like [Cucurbita moschata])

HSP 1 Score: 343.2 bits (879), Expect = 2.1e-90
Identity = 194/366 (53.01%), Postives = 226/366 (61.75%), Query Frame = 0

Query: 1   MTRITYHIAALSVLHVIW----------------------LLFFSKPFDTGNNNFISIIF 60
           M R+ Y IAALS L++IW                      L+F     DTGNNNFIS IF
Sbjct: 1   MARVNYLIAALS-LYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYSSYVTDFPGH------------------------FVPPFLDPKLSDDDVKIGVSFA 120
           KANYS Y  DFPGH                         VPPFLDPKLS DDVK GVSFA
Sbjct: 61  KANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFA 120

Query: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLNESRKILDSALVVISAGTND- 180
           SAG+GFD+LTA++S VIPVMKQID+FKNYTQRLQ I G++ES+KI+ SALV+ISAGTND 
Sbjct: 121 SAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDL 180

Query: 181 -------------YNISGYQDFLQNRLQSLIK-----------VIGLPPVGCLPIHESIA 240
                        YNISGYQDF+ NRLQS IK           V GLPPVGCLPI E+++
Sbjct: 181 NINFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVS 240

Query: 241 FQ------------------NLKIENVWKN-KTQIPKPTIRSFFLEAQLYADIYTPLIDM 277
           F+                  N K+  +  N ++Q+P  T+        LYADIYTPLIDM
Sbjct: 241 FENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTM--------LYADIYTPLIDM 300

BLAST of PI0005574 vs. TAIR 10
Match: AT2G30220.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-55
Identity = 125/323 (38.70%), Postives = 175/323 (54.18%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNFIS-IIFKANYSSYVTDFPGH------------------------F 78
           L+F     DTGNNN+ S  +FKAN+  Y  D PGH                        F
Sbjct: 35  LIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLNIKEF 94

Query: 79  VPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGL 138
           VPPFL P +SD D+  GV FASAG+G+D+ T+  S  IPV +Q  MFKNY  RL+GIVG 
Sbjct: 95  VPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKGIVGD 154

Query: 139 NESRKILDSALVVISAGTNDY---------------NISGYQDFLQNRLQSLIK------ 198
            ++ +I+++ALVVISAG ND+                I GYQDF+  RL   ++      
Sbjct: 155 KKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVRELYSLG 214

Query: 199 -----VIGLPPVGCLPIHES--------IAFQNLKIENVWKNKTQIPK-PTIRSFFLEAQ 258
                V GLPP+GCLPI  +        I  +    +++  N+  + K P I++    ++
Sbjct: 215 CRNILVGGLPPMGCLPIQLTAKLRTILGICVEQENKDSILYNQKLVKKLPEIQASLPGSK 274

Query: 259 -LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDN 281
            LYA++Y P++DM+ NP  YGF+    GCCG G +E   LC + + T C N S  +FWD+
Sbjct: 275 FLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTSLSKT-CPNHSDHLFWDS 334

BLAST of PI0005574 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 209.5 bits (532), Expect = 3.4e-54
Identity = 121/317 (38.17%), Postives = 166/317 (52.37%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNFISIIFKANYSSYVTDFPGH------------------------FV 78
           L+F     DTGNNN+I    +AN+  Y  +FPGH                         V
Sbjct: 39  LVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGIKDTV 98

Query: 79  PPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGLN 138
           PPFLDP LSD D+  GV FASAGSG+D LT   ++ + V KQ DM ++Y +RL  IVG  
Sbjct: 99  PPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQIVGDE 158

Query: 139 ESRKILDSALVVISAGTNDYN--------------ISGYQDFL----QNRLQSL------ 198
           ++  I+  ALV++S+GTND+N              + GYQ F+     N +Q L      
Sbjct: 159 KAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYDIGCR 218

Query: 199 -IKVIGLPPVGCLPIHESIAFQ---NLKIENVWKNKTQIPKPTIRSFFLEAQ-------- 258
            I V+GLPPVGCLPI  ++A Q     +  +   + +Q     +++   E Q        
Sbjct: 219 KIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLTGSVI 278

Query: 259 LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDNI 276
            Y DIY  L DM  NP  YG +    GCCG G +E   LCN  T  IC NP++++FWD+I
Sbjct: 279 FYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALT-RICPNPNQYLFWDDI 338

BLAST of PI0005574 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 209.5 bits (532), Expect = 3.4e-54
Identity = 123/311 (39.55%), Postives = 168/311 (54.02%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNFIS-IIFKANYSSYVTDFPGH------------------------F 78
           L+F     DTGNNN+ S  IFKA +  Y  D PGH                         
Sbjct: 36  LIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLNIKEL 95

Query: 79  VPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGL 138
           VPPFL P +S  D+  GVSFASAG+G+D+ ++  S  IPV +Q  MFKNY  RL+GIVG 
Sbjct: 96  VPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKGIVGD 155

Query: 139 NESRKILDSALVVISAGTNDY---------------NISGYQDFLQNRLQSLIK------ 198
            ++ +I+++ALVVISAG ND+                I GYQ+F+  RL   ++      
Sbjct: 156 KKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVRELYSLG 215

Query: 199 -----VIGLPPVGCLPIHESIAFQNL--------KIENVWKNKTQIPK-PTIRSFFLEAQ 258
                V GLPP+GCLPI  +   +N+          ++V  N+  + K P I++    + 
Sbjct: 216 CRNIVVGGLPPMGCLPIQMTAKMRNILRFCVEQENKDSVLYNQKLVKKLPEIQASLPGSN 275

Query: 259 -LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDN 269
            LYA++Y PL+DM+ NP  YGF+    GCCG G +E   +CN  T T C N S  +FWD+
Sbjct: 276 FLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLTKT-CPNHSDHLFWDS 335

BLAST of PI0005574 vs. TAIR 10
Match: AT2G31540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 208.8 bits (530), Expect = 5.8e-54
Identity = 118/311 (37.94%), Postives = 171/311 (54.98%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNF-ISIIFKANYSSYVTDFPG------------------------HF 78
           L+F     DTGNNN+ +  IF+A +  Y  D P                          F
Sbjct: 37  LIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLNIKEF 96

Query: 79  VPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGL 138
           +PPFL P LSD D+  GV FASAG+G+D+LT+  +  I V +Q +MFK+Y  RL+GIVG 
Sbjct: 97  IPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKGIVGD 156

Query: 139 NESRKILDSALVVISAGTNDY---------------NISGYQDFLQNRLQSLIK------ 198
            ++ +I+++A VV+SAG ND+                ISGYQDF+  RL++ ++      
Sbjct: 157 KKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVRELYSLG 216

Query: 199 -----VIGLPPVGCLPIHESIAFQNL---------KIENVWKNKTQIPKPTIRSFFLEAQ 258
                V GLPP+GCLPIH +  F+N+         K   ++  K Q   P I +    ++
Sbjct: 217 VRNVLVGGLPPMGCLPIHMTAKFRNIFRFCLEHHNKDSVLYNEKLQNLLPQIEASLPGSK 276

Query: 259 -LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDN 269
            LYAD+Y P+++M+ NP  YGF+    GCCG G +E   +C N  S +C+N S+F+F+D+
Sbjct: 277 FLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSFMC-NVFSPVCQNRSEFLFFDS 336

BLAST of PI0005574 vs. TAIR 10
Match: AT1G58430.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-52
Identity = 118/311 (37.94%), Postives = 174/311 (55.95%), Query Frame = 0

Query: 19  LLFFSKPFDTGNNNFIS-IIFKANYSSYVTDFPGH------------------------F 78
           L+F     DTGNNN+ S  IF+A +  Y  D P H                        F
Sbjct: 37  LIFGDSTVDTGNNNYPSQTIFRAKHVPYGIDLPNHSPNGRFSNGKIFSDIIATKLNIKQF 96

Query: 79  VPPFLDPKLSDDDVKIGVSFASAGSGFDELTASVSNVIPVMKQIDMFKNYTQRLQGIVGL 138
           VPPFL P L+D ++  GV FASAG+G+D+ T+  +  I V +Q +MFK+Y  RL+ IVG 
Sbjct: 97  VPPFLQPNLTDQEIVTGVCFASAGAGYDDQTSLTTQAIRVSEQPNMFKSYIARLKSIVGD 156

Query: 139 NESRKILDSALVVISAGTNDY---------------NISGYQDFLQNRLQSLIK------ 198
            ++ KI+++ALVV+SAG ND+               +IS YQDF+ +RL + +K      
Sbjct: 157 KKAMKIINNALVVVSAGPNDFILNYYEVPSWRRMYPSISDYQDFVLSRLNNFVKELYSLG 216

Query: 199 -----VIGLPPVGCLPIHESIAFQN-----LKIEN----VWKNKTQIPKPTIRSFFLEAQ 258
                V GLPP+GCLPI  +  F+N     L+ EN    ++  K Q   P  ++    ++
Sbjct: 217 CRKILVGGLPPMGCLPIQMTAQFRNVLRFCLEQENRDSVLYNQKLQKLLPQTQASLTGSK 276

Query: 259 -LYADIYTPLIDMVNNPHNYGFEHVNVGCCGIGMVEAGPLCNNKTSTICENPSKFMFWDN 269
            LY+D+Y P+++M+ NP  YGF+    GCCG G +E   +C N  S++C+N S+F+F+D+
Sbjct: 277 ILYSDVYDPMMEMLQNPSKYGFKETTRGCCGTGFLETSFMC-NAYSSMCQNRSEFLFFDS 336