PI0005528 (gene) Melon (PI 482460) v1

Overview
NamePI0005528
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionProtein of unknown function (DUF803)
Locationchr07: 23392384 .. 23395127 (-)
RNA-Seq ExpressionPI0005528
SyntenyPI0005528
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGAACCGATTTGCTTAACTTTGGCCGCAGCTGCCGGCAACAATATCGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTACGCTTTCTCCTTTTTCTTTCTATTGCCTTTGCTTTCCTTCTCACATTTCCTTCTGGGTCATTTAATTTCACGCGTTTCGTTTTCTTTTTCTTCCTTGAACTGTCCGATCGCTAGCACTATGGTGTTTCTTTGATTCTCAATTTTTCGGATTTCATTAAGGATTTGAGCGAATTTTATTTTCTCTTTCTTTTGGTGAATTCCTTGAATGAAGTTGTGAATTCTCATTGGTTTTTTTTGTTATATAGGTGATAAGAGCTTACGCTTTTAACAAAACTTGGATCATTGGTTTTCTGATGGATATATTCGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTAAGTTCTTTTATTCAGCTACATATGTCATTTTCTTTTCGGTTTTTTGATTGGCATTCGATGATTGGATCTTGGCATTATTGAGCGAATAGGCTGTTTTTGTTCTGAGATCTAGATGAATTTAGCGCGCCTTTATAGTCTCCCTACAGGCTACATTTGATGGAAAATATCAATTGCTGTTTTGATGGCTAACTGTCTCCTTTTGTCGTATTTATTATATCGCATCTATCTCTATGAAAATTACATATAATCTAAGATTCTAAATATTTCATAGATTTTTCCCCCCAAGAATCTGTACGTCACAGGAAAATTTATACGGTTCTGTCGTTGTATGTTGCGCACATTTTCGTCTCCTAATATGCATTTCCTTTCTAATTTTGAATTTCAGGTTTCTATAATTCAACCAGTTTCTGGGTGTGGACTTGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGCTGTTGATTGGATGGGGATTATGCTTGCTGGTATTGGCACCATAGGTAATGTAAGTTCATCTTTGTGTCTTTAATCTAGTTATTGTTTGATCTCTTGATCTCTATTTTATGCATTTCAGGTTTGGCCATGCCTTGTATGCCAACTTTTTATGAATCTTCATCTTCCTTGCCCACATTTGTAATTTATTTGGCCATTTTCTTTTTTCTCCCTTTTTTTGCCATTTCTAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCATCTGCCATATCAGTGTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTAGGTATCTTATATATTATTATAGCCCCACGTTTATAACAATCTCTGGTCATTTATGTTAGCAGTTTTATGGGCTTGCTTGCAGAAACAAATATAACATACTAGTACTTTGTAGATAAGGCTGTAAAAGATGAGTGAATTGACATCTAGAAATGATTATTTTAACAGGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGGTAAATTCATTGCTTTTATATTGACATTTCAATTGTTTTCTTTTTCCTCCCAGAAAAATTATATGTTACATATGTATATATGTTGCAGATGGAATTTGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTCGGGTACTGTTCTTGTTTGCACTCTTTTACTTGATGGATTACATTTCCTTCAATCATTAAGGTTGTTGAGGATGACTATTAGGAATGAGAATTTTGCTTCACTGTGAGGAATTAGGGCTTCTGCCTGACTGTGGTGGTATCCTAGTTGTTTCAAATTGAATCTTAGCCAACTTTTATTTTGACTTCAGGATGTACTAGGCATTGAAAAGAAGAATTTTGAATAATCATTGTTGTGGTTTTCTGGAACATAAGCATACCATAGAATTGTTAGTTTAGTCTTTTACACATATATGGCATAAATATAGAATGGAAATAATCCAATTGGCCTTTTAGATTCATAATAGCAAAATTCACAAGTTAAATCTACAATTGCAGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTGATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGGTACTTCAAATGCTTGGTGATTTGATCTCTTTACGATGCTATTTTGCACTCAGTGTTAGTTGTTTTATCAACAACTTCTGTCTGTGTTCTTCTAGTTCATAATAATGTTGTCTTTTTTTTTAGTAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATCGTCACTGGTGTCGTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCGTCGCCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGTAAACTACCTTAACTATAATTCCCTTTCGAGTTTACGATGACTGACCAGACTTGATAAAGAGTGGTTTCTGGCCATGTGAATCCATCGTCACTATGTGGGTTAATGTTATTTAATTCAAGATATTCTCTGTACAGGCTGCTAATCATCGTAGGTGTTATTCTGCTGGTGAGTTCCTCACGACTGATCCGACGTCTTACTTGGCTATACCGGCGCTTTAAGCGAAGTAGTGTTGATAGGAACTTTGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGATTCAAGCCCAAGTGCAATCATTCAGACAACGACGTTGCAAAATTTGTTATCTTCAAAAGCAAAGGCTGACGCTTGA

mRNA sequence

ATGTGGGAACCGATTTGCTTAACTTTGGCCGCAGCTGCCGGCAACAATATCGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTGATAAGAGCTTACGCTTTTAACAAAACTTGGATCATTGGTTTTCTGATGGATATATTCGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTTTCTATAATTCAACCAGTTTCTGGGTGTGGACTTGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGCTGTTGATTGGATGGGGATTATGCTTGCTGGTATTGGCACCATAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCATCTGCCATATCAGTGTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGATGGAATTTGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTCGGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTGATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATCGTCACTGGTGTCGTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCGTCGCCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGCTGCTAATCATCGTAGGTGTTATTCTGCTGGTGAGTTCCTCACGACTGATCCGACGTCTTACTTGGCTATACCGGCGCTTTAAGCGAAGTAGTGTTGATAGGAACTTTGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGATTCAAGCCCAAGTGCAATCATTCAGACAACGACGTTGCAAAATTTGTTATCTTCAAAAGCAAAGGCTGACGCTTGA

Coding sequence (CDS)

ATGTGGGAACCGATTTGCTTAACTTTGGCCGCAGCTGCCGGCAACAATATCGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTGATAAGAGCTTACGCTTTTAACAAAACTTGGATCATTGGTTTTCTGATGGATATATTCGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTTTCTATAATTCAACCAGTTTCTGGGTGTGGACTTGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGCTGTTGATTGGATGGGGATTATGCTTGCTGGTATTGGCACCATAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCATCTGCCATATCAGTGTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGATGGAATTTGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTCGGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTGATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATCGTCACTGGTGTCGTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCGTCGCCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGCTGCTAATCATCGTAGGTGTTATTCTGCTGGTGAGTTCCTCACGACTGATCCGACGTCTTACTTGGCTATACCGGCGCTTTAAGCGAAGTAGTGTTGATAGGAACTTTGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGATTCAAGCCCAAGTGCAATCATTCAGACAACGACGTTGCAAAATTTGTTATCTTCAAAAGCAAAGGCTGACGCTTGA

Protein sequence

MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAVLMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFKRSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA
Homology
BLAST of PI0005528 vs. ExPASy Swiss-Prot
Match: Q8RWH8 (Probable magnesium transporter NIPA9 OS=Arabidopsis thaliana OX=3702 GN=At5g11960 PE=2 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 1.5e-141
Identity = 255/341 (74.78%), Postives = 299/341 (87.68%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWE ICLTLAA AGNNIGK+LQKKGT+ILPPLS KLKV+RAYA NK W +GFLMDI GA+
Sbjct: 1   MWESICLTLAATAGNNIGKVLQKKGTIILPPLSLKLKVLRAYAENKPWALGFLMDIVGAL 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVS++QPVSGCGLAILS+FSHFYLKE+MN  DW+GI +AGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSVVQPVSGCGLAILSVFSHFYLKEVMNVFDWIGITVAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQ+AS ISVF L WLA ++ ILFVLLN WLH +KRQRREQEL E+EVVEEIIYGLESGIL
Sbjct: 121 EQEASLISVFQLLWLALVVAILFVLLNAWLHIFKRQRREQELGEYEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASV+SKMGF+F+EQGF  + +P+CI ISICCS TGF+YQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVVSKMGFVFVEQGFSTMFIPMCISISICCSGTGFFYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVVAGM ALGE+LP+SP+GRL LLLGWLLI++GV+LLV+SSRLIR L   +RR +
Sbjct: 241 ASIVTGVVAGMFALGEKLPTSPSGRLLLLLGWLLIMLGVVLLVTSSRLIRHLPRSFRRSR 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAK 342
           ++S++R F  R  ++   +D++PSA+IQ  TL +LLSS +K
Sbjct: 301 QTSLERGFNIRRTTSHTPKDTNPSAVIQAATLHHLLSSPSK 341

BLAST of PI0005528 vs. ExPASy Swiss-Prot
Match: P55580 (Uncharacterized protein y4nH OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a02340 PE=4 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 7.8e-05
Identity = 37/117 (31.62%), Postives = 54/117 (46.15%), Query Frame = 0

Query: 4   PICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAVLML 63
           PI L  AA   + IG IL K G   LPP +          F    + G +      +L  
Sbjct: 5   PIILVFAAGLNSCIGNILLKWGRASLPPSAGLADTFLTPGF----VGGVVFYGINVLLFA 64

Query: 64  RALSLAPVSIIQPV-SGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGG 120
           +AL    VS+  P+ +G G A+L I SH++  E  +   W+G+ L  +G I +  GG
Sbjct: 65  KALDSLEVSVAYPILAGSGFAMLIIASHYFFGEPFHLHKWIGVALVLVGIIFLARGG 117

BLAST of PI0005528 vs. ExPASy Swiss-Prot
Match: Q8BGN5 (NIPA-like protein 3 OS=Mus musculus OX=10090 GN=Nipal3 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.0e-04
Identity = 65/268 (24.25%), Postives = 113/268 (42.16%), Query Frame = 0

Query: 40  RAYAFNKTWIIGFLMDIFGAVLMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNA 99
           RAY   KTW +G L+ + G + +  + + AP+S+I P+S   +   +I    ++KE    
Sbjct: 72  RAYFKTKTWWLGLLLLLLGELGVFASYAFAPLSLIVPLSAVSVIASAIIGIIFIKEKWKP 131

Query: 100 VD--------WMGIMLAGIGT---IGVGAGGEEQKASAISVFHL---PWLAFIMT--ILF 159
            D        ++G  LA +GT   +       E+        HL   P+L +++   +LF
Sbjct: 132 KDFVRRYVLSFVGCGLAIVGTYLLVTFAPNSHEKMTGENIARHLVSWPFLLYMLVAIVLF 191

Query: 160 VLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQIL 219
            LL   L+FYK +          VV  ++  L   +       +S M  L + QG  Q+ 
Sbjct: 192 CLL---LYFYKERNANS-----IVVILLLVALLGSMTVVTVKAVSGMLVLSI-QGNLQLD 251

Query: 220 VPICILISICCSATGFYYQTRGLKHGRAI----VVSTCAAVASIVTGVVAGMLALGEELP 279
            PI  ++ +C  AT   YQ   L     I    ++++   + S    + AG +   + L 
Sbjct: 252 YPIFYVMFVCMVATAI-YQATFLSEASQIYDSSLIASVGYILSTTAAITAGAIFYLDFLG 311

Query: 280 SSPTGRLFLLLGWLLIIVGVILLVSSSR 288
                     LG L+  +GV L+  + +
Sbjct: 312 EEALHICMFALGCLIAFLGVFLITRNRK 329

BLAST of PI0005528 vs. ExPASy TrEMBL
Match: A0A0A0L5B6 (Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_4G646200 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.3e-184
Identity = 340/344 (98.84%), Postives = 342/344 (99.42%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGIMLAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGMLALGEELPSSPTGRLFLLLGWLLII+GVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           RS VDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of PI0005528 vs. ExPASy TrEMBL
Match: A0A5A7VA96 (Probable magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G002250 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.8e-183
Identity = 337/344 (97.97%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGMLALGEELPSSPTGRLFLLLGWLLII+GVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           R+ VDRNFGHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of PI0005528 vs. ExPASy TrEMBL
Match: A0A1S3BUH9 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103493671 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.8e-183
Identity = 337/344 (97.97%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGMLALGEELPSSPTGRLFLLLGWLLII+GVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           R+ VDRNFGHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of PI0005528 vs. ExPASy TrEMBL
Match: A0A6J1FDL9 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111443049 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.0e-177
Identity = 329/345 (95.36%), Postives = 339/345 (98.26%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKI+QKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKIIQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGITLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAIS+FHLPWLAFI+TILFVLL+GWLHFYKRQR+EQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISIFHLPWLAFIITILFVLLSGWLHFYKRQRKEQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFH+ILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVVAGMLALGEELPSSPT RLFLLLGWLLII GVILLVSS+RL RRLTWLYRRFK
Sbjct: 241 ASIVTGVVAGMLALGEELPSSPTARLFLLLGWLLIITGVILLVSSARLTRRLTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQN-LLSSKAKADA 345
           RS VDR+FGHRSGS+VRLRDSSPSAIIQTTTLQN +LSSKAKADA
Sbjct: 301 RSGVDRSFGHRSGSSVRLRDSSPSAIIQTTTLQNMMLSSKAKADA 345

BLAST of PI0005528 vs. ExPASy TrEMBL
Match: A0A6J1IIP5 (Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111477247 PE=3 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.1e-176
Identity = 327/345 (94.78%), Postives = 337/345 (97.68%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKI+QKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKIIQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGITLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQK SAIS+FHLPWLAFI+TILFVLL+GWLHFYKRQR+EQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKVSAISIFHLPWLAFIITILFVLLSGWLHFYKRQRKEQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFH+ILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVVAGMLALGEELPSSPT RLFLLLGWLLII GVILLVSS+RL R LTWLYRRFK
Sbjct: 241 ASIVTGVVAGMLALGEELPSSPTARLFLLLGWLLIITGVILLVSSARLTRHLTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQN-LLSSKAKADA 345
           RS VDR+FGHRSGS+VRLRDSSPSAIIQTTTLQN +LSSKAKADA
Sbjct: 301 RSGVDRSFGHRSGSSVRLRDSSPSAIIQTTTLQNMMLSSKAKADA 345

BLAST of PI0005528 vs. NCBI nr
Match: XP_004141325.1 (probable magnesium transporter NIPA9 isoform X2 [Cucumis sativus] >KGN55346.1 hypothetical protein Csa_012133 [Cucumis sativus])

HSP 1 Score: 654.1 bits (1686), Expect = 6.9e-184
Identity = 340/344 (98.84%), Postives = 342/344 (99.42%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGIMLAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGMLALGEELPSSPTGRLFLLLGWLLII+GVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           RS VDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of PI0005528 vs. NCBI nr
Match: XP_008452740.1 (PREDICTED: probable magnesium transporter NIPA9 [Cucumis melo] >KAA0064508.1 putative magnesium transporter NIPA9 [Cucumis melo var. makuwa])

HSP 1 Score: 650.2 bits (1676), Expect = 9.9e-183
Identity = 337/344 (97.97%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGV+AGMLALGEELPSSPTGRLFLLLGWLLII+GVILLVSSSRLIRRLTWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 345
           R+ VDRNFGHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of PI0005528 vs. NCBI nr
Match: XP_038896971.1 (probable magnesium transporter NIPA9 [Benincasa hispida])

HSP 1 Score: 641.7 bits (1654), Expect = 3.5e-180
Identity = 334/345 (96.81%), Postives = 339/345 (98.26%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGA+
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAI 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGIMLAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAISVFHLPWLAFIMTILF+LLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFILLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFH+ILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASI+TGVVAGMLALGEELPSSPTGRL LLLGWLLIIVGVILLVSSSRLIRR TWLYRRFK
Sbjct: 241 ASIITGVVAGMLALGEELPSSPTGRLLLLLGWLLIIVGVILLVSSSRLIRRFTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNL-LSSKAKADA 345
           RS VDRNFGHRSGS+VRLRDSSPSAIIQTTTLQNL LSSK KADA
Sbjct: 301 RSGVDRNFGHRSGSSVRLRDSSPSAIIQTTTLQNLMLSSKEKADA 345

BLAST of PI0005528 vs. NCBI nr
Match: XP_031740158.1 (probable magnesium transporter NIPA9 isoform X1 [Cucumis sativus])

HSP 1 Score: 637.5 bits (1643), Expect = 6.7e-179
Identity = 340/376 (90.43%), Postives = 342/376 (90.96%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTI------- 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGIMLAGIGTI       
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGNVWPCL 120

Query: 121 -------------------------GVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG 180
                                    GVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG
Sbjct: 121 VCQVFINIHLPCTHLQFTWLFSPFLGVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG 180

Query: 181 WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI 240
           WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI
Sbjct: 181 WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI 240

Query: 241 LISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVVAGMLALGEELPSSPTGRLFL 300
           LISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGV+AGMLALGEELPSSPTGRLFL
Sbjct: 241 LISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFL 300

Query: 301 LLGWLLIIVGVILLVSSSRLIRRLTWLYRRFKRSSVDRNFGHRSGSAVRLRDSSPSAIIQ 345
           LLGWLLII+GVILLVSSSRLIRRLTWLYRRFKRS VDRNFGHRSGSAVRLRDSSPSAIIQ
Sbjct: 301 LLGWLLIIIGVILLVSSSRLIRRLTWLYRRFKRSGVDRNFGHRSGSAVRLRDSSPSAIIQ 360

BLAST of PI0005528 vs. NCBI nr
Match: XP_022936425.1 (probable magnesium transporter NIPA9 [Cucurbita moschata] >XP_023535010.1 probable magnesium transporter NIPA9 [Cucurbita pepo subsp. pepo] >KAG6591472.1 putative magnesium transporter NIPA9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 632.5 bits (1630), Expect = 2.1e-177
Identity = 329/345 (95.36%), Postives = 339/345 (98.26%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWEPICLTLAAAAGNNIGKI+QKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKIIQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGITLAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQKASAIS+FHLPWLAFI+TILFVLL+GWLHFYKRQR+EQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISIFHLPWLAFIITILFVLLSGWLHFYKRQRKEQELMEFEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASVISKMGFLFLEQGFH+ILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVVAGMLALGEELPSSPT RLFLLLGWLLII GVILLVSS+RL RRLTWLYRRFK
Sbjct: 241 ASIVTGVVAGMLALGEELPSSPTARLFLLLGWLLIITGVILLVSSARLTRRLTWLYRRFK 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQN-LLSSKAKADA 345
           RS VDR+FGHRSGS+VRLRDSSPSAIIQTTTLQN +LSSKAKADA
Sbjct: 301 RSGVDRSFGHRSGSSVRLRDSSPSAIIQTTTLQNMMLSSKAKADA 345

BLAST of PI0005528 vs. TAIR 10
Match: AT5G11960.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 503.8 bits (1296), Expect = 1.1e-142
Identity = 255/341 (74.78%), Postives = 299/341 (87.68%), Query Frame = 0

Query: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60
           MWE ICLTLAA AGNNIGK+LQKKGT+ILPPLS KLKV+RAYA NK W +GFLMDI GA+
Sbjct: 1   MWESICLTLAATAGNNIGKVLQKKGTIILPPLSLKLKVLRAYAENKPWALGFLMDIVGAL 60

Query: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGIMLAGIGTIGVGAGGE 120
           LMLRALSLAPVS++QPVSGCGLAILS+FSHFYLKE+MN  DW+GI +AGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSVVQPVSGCGLAILSVFSHFYLKEVMNVFDWIGITVAGIGTIGVGAGGE 120

Query: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180
           EQ+AS ISVF L WLA ++ ILFVLLN WLH +KRQRREQEL E+EVVEEIIYGLESGIL
Sbjct: 121 EQEASLISVFQLLWLALVVAILFVLLNAWLHIFKRQRREQELGEYEVVEEIIYGLESGIL 180

Query: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240
           FGMASV+SKMGF+F+EQGF  + +P+CI ISICCS TGF+YQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVVSKMGFVFVEQGFSTMFIPMCISISICCSGTGFFYQTRGLKHGRAIVVSTCAAV 240

Query: 241 ASIVTGVVAGMLALGEELPSSPTGRLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300
           ASIVTGVVAGM ALGE+LP+SP+GRL LLLGWLLI++GV+LLV+SSRLIR L   +RR +
Sbjct: 241 ASIVTGVVAGMFALGEKLPTSPSGRLLLLLGWLLIMLGVVLLVTSSRLIRHLPRSFRRSR 300

Query: 301 RSSVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAK 342
           ++S++R F  R  ++   +D++PSA+IQ  TL +LLSS +K
Sbjct: 301 QTSLERGFNIRRTTSHTPKDTNPSAVIQAATLHHLLSSPSK 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWH81.5e-14174.78Probable magnesium transporter NIPA9 OS=Arabidopsis thaliana OX=3702 GN=At5g1196... [more]
P555807.8e-0531.62Uncharacterized protein y4nH OS=Sinorhizobium fredii (strain NBRC 101917 / NGR23... [more]
Q8BGN53.0e-0424.25NIPA-like protein 3 OS=Mus musculus OX=10090 GN=Nipal3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L5B63.3e-18498.84Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_4G646200 PE=3 S... [more]
A0A5A7VA964.8e-18397.97Probable magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A1S3BUH94.8e-18397.97Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103493671 PE=3 SV=1[more]
A0A6J1FDL91.0e-17795.36Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111443049 PE=... [more]
A0A6J1IIP51.1e-17694.78Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111477247 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_004141325.16.9e-18498.84probable magnesium transporter NIPA9 isoform X2 [Cucumis sativus] >KGN55346.1 hy... [more]
XP_008452740.19.9e-18397.97PREDICTED: probable magnesium transporter NIPA9 [Cucumis melo] >KAA0064508.1 put... [more]
XP_038896971.13.5e-18096.81probable magnesium transporter NIPA9 [Benincasa hispida][more]
XP_031740158.16.7e-17990.43probable magnesium transporter NIPA9 isoform X1 [Cucumis sativus][more]
XP_022936425.12.1e-17795.36probable magnesium transporter NIPA9 [Cucurbita moschata] >XP_023535010.1 probab... [more]
Match NameE-valueIdentityDescription
AT5G11960.11.1e-14274.78Protein of unknown function (DUF803) [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 40..283
e-value: 1.9E-8
score: 33.8
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 1..341
NoneNo IPR availableGENE3D1.10.3730.20coord: 5..118
e-value: 1.6E-6
score: 30.3
NoneNo IPR availablePANTHERPTHR12570:SF65MAGNESIUM TRANSPORTER NIPA9-RELATEDcoord: 1..341
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 190..287
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 25..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005528.1PI0005528.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0015693 magnesium ion transport
cellular_component GO:0005769 early endosome
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity