PI0005459 (gene) Melon (PI 482460) v1

Overview


Name	PI0005459
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	(S)-2-hydroxy-acid oxidase
Location	chr04: 15543207 .. 15547876 (+)
RNA-Seq Expression	PI0005459
Synteny	PI0005459

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAGACTTCATCTTCTAAGAGAATTATACATTAGGATAGTTGGTGTCATATTAATTTTGCTACCCAATTCCTCTGTTTTTAAAGTTCCAGCCATGAATCTGGGAAATTTCTATCTTCCCTTCCTCAAGAAGTGGATTTATATGGTTTTAGAGACTATGGCAGTATTATCCAAGGTATTTTCTTTAGGGTTTTTGGCTAAAATAAGTAAGAGAGAGTGTAGTTCAATGGTTGAACTGATGTGGGCAAGATCTTGTAACTATGCTTTTTGAGTTTTCATTTCATTGTGGTGTATAGATCTTGACTTTGGCTGATATTTGTTTGAATGGATTTTCATGGTTACTAAAAGCTCTAAAAAGGTATTCTTCTCTTCTTGTCTCTATTTAATCAGGATTAAAATGTCAACCGAACCAGTGAATGTGGATGAGTTCGAAGAATTGGCTAGGCAGGCACTTCCAAGAATGTATTACGATTACTATGCAGGAGGAGCTGAGGACCAGCACACACTTAGACAGAACATACAAGCTTTCTGTAGAATCACGTAATCTTTTTTTAAAGCTAGTTTGTGATTCTCTGTGAAAAGGGATACTATATGTTGTTAATAATTTTCAATTGTTTTCAGGATACAACCTCGAATTCTTGTTGACGTGAGTAAAATTGATATGTCAACAACGATCTTGGGCTATCGTATCTCTGCACCTATTATGGTTGCTCCAACCGGTGCACATAAGCTGGCATACCATGAAGGTTGAACTATGGTGAATTGCTTCGTTGAGATATGGTTTTCATTCAACTCTTCCTTGTTGATATTTGTGTTCTTTCAGGAGAGTTAGCCACAGCCAGAGCAGCTGCTGCTGCAAAAACTATAATGGTACCGATGTGACTACAACTAGACTAGAAGTTTTTTTTTTTTTTTTTTTTTTTTCTCATTCAATTTAGTGTTTGAATTTAAATGGTTTTGTTTGTAGGTTCTATCCTTTGGTTCCTCCTACTCAATGGAGGAAGTTGCCTCTAGCTGCAATGCTATCCGTTTCTTTCAATTATATGTAAATGGCTCTTCCTTATCCCTTTACATTTGGCTTTTTCAATCTTTTCCTATAGCAGGAATCCAATTGTTTGCAAGTTACTGAAGTTTTTTTCTTTCTGTAATTAGGTTTTCAAATGGCGTGATATCTCGACTATGCTAGTACAGAGAGCTGAGAGATTTGGATATAAGGCCATTGTTCTGACTGCTGATACGCCTCGACTTGGTAAAAGGGAGGCTGATATAAAGAACAAGTAGCAATGCTTCACATCTTTGGACATGTTTTTGGAAGTGATTTTATGAATAGTAATTTTAGTGGAAATAATTTGTTTTTAAGTGATTTATAATCATTTCGTGTTTGATTTTATACTTTCAAATACGTTTTTATTCTTTTAAAATTCATTATGAATGGCTAAGTACATGTTTGAGAGTTACTCTGAGTAAGAGTAATTTAACCATGTCAAAATCACTTCAAAACATGTCATAGTTTCTTTCCTATCTAATGTGTTTCATTTCATTTACTGAGTATAAAATTTGTCATTGTATGGAAAAAATTCATATTCCTGTATCAAACTGTTAAGTCCTGAGATAGGATCTGTTCTTCCTTTTATCTCACATCAAACTGGTAAACTGTACCTTGATTTTGTCTCATCTAGAATAGTACTTGGATGAATTTGCAGGATGATTACACCACCACAGAAGAATTTTGAAGGCTTGTTCTCTGTTAAAGTTGAAGTTGAGTCTGTGAGTAGTATTTAAATTTCCTATTTAGAATCTTGTAAGTACTAGTTTAATCTAACAAATTTTCAAACTGACTTATATCCACGGCATGTTGTACCACTTGGTTGTATTTCCCTGCTGTATAGTTTAGTTATACTGAGTTGCATGCTTGTACGATTATCTCGACCAATAATTTTGGTTATTCATACATCTGGCTCCAAGTTTAATTCGGTTATTTTTCTTCGAGTATTGTGCTATTCTTGGGTTTATTGTATAATAAACTTAACTTGTGATCTTATGCAGGATCAAGGTTCGTTATTAGAGTCTTTTCTTAATGAGGCCTTTGACCCATCCCTTTGTTGGAAAGTAAGTCCGATAAACTGTTATGATGTCTATGTATAACTTGTCTGCTTATGAAAAATTTATTAAATTTTGGTGGCTATTCTTCAAGTAACTCAATAACTTAACTACTTCTATGTTATATAAGAAGCTCTAGTAATATGATCTCTTTAAGAAACTTCTACTACTAAAAAGTATTGTTTGCATGACTTTTTATGTGCATGATTTTACTGGCTCAGTTATGATATTGTACCATCCACAGGATATAGCATGGTTGAAGTCAATTACTAGCTTGCCAATTCTGATAAAGGGAATTCTCACTCATGAAGATGGTAAGATTACAATTATGTCTTAAAGACATAAGAATCAGAAAATGAACTTTTCTTTTCCCTATCTGATGAAATGTTCTGCAATAACATATCTTGATATTAAATTCAAATTCTGCAAGCAAGACCTCGTTTAATCTCGCAATTAAGTTTCTATATGTTGACCGAAAGAAAATGCATGATTATATGTACCCTTTTCCTTCTTTTCTCTTAATCAGTCTCTATCAGGTTCGTGTGTCGTGCCATTGTCCGTGTATAGCCCTTTTTTTAATTTGTTAACGTGGTTCATAAATGTAGCAATTAAGGCTGTGGAAGTAGGTGTTGATGGAATTATTGTCTCCAATCATGGAGCTCGCCAACTAGATTTTTCTCCTGCCACCATTTCTGTTCTTGAAGAGGTAAATCTCTTTTCTTACTAATTTATGATTAGCTAAAGATGTCGATACAAACCGGTACCTTGATTTCAGAATAAAATTATTGCACAGTACCTATGTTCCCTCAAGGATGAACCGAGTATCGTATCTGCTCGTAGATGTTAAACAGGCATCACTGCATCCAAATATTTTTTCACAACTTTCTTTTCTCTGGTTGCTATCTTCTCTCATTTATTTGCACATTCTTTCAAATTGGTGAATGTTCACATTTGTGTCTTGTATGATGCATTTCTCATAGTAAGTGATATGTGATAGCAATATATTATCTCCTCTCCTTCATTTGCACATTCTTTCAAAATTTACATGCTTACTTTTCCATTTACTATAATCTTTTAACTCGTTTATTAAGCAATAAGAATAAGTATTGGAAGAACGGTTTTTCAACATAGAAAGCAAATTGCGGGTAGTTAATGCTGTGAAGGGTAAAGTTCCTGTGCTATTAGATGGAGGTGTGAGGCGAAGAACAGATGTGTTTAGGGCATTAGCCCTTGGTGCACAGGCAGTCCTTGTAAGCTCTCCAACTTTCAATTAGCCTTCTAGGGTATGTATAATATAATTCATGTTTCTTACTTTCACTTTATACTTCTATAGAAAATTGTACAGTTTTGTCTTAATGGTCAACTCGTTCATGTAACCAAGATAGAGATGATACAAACAATGGAACGTTTACCTACCGTCTACTTTTGGCATAAAGTCACATGACCTTACTTTTGGAACCACCTAAAGAGTTTTATTTCAGTTGAGATAATTGTCTTCAAAATTACCCCAACTCATAAACTTCAATACCATCCCGTTAAGGACAGGTTTTCTAGTAAAGCAGGAGGTTTGCTCGTACTCCTATAATATCCTTTCCTTTTTAGTTTTGCAAATTAATCAATTTACACTCAATTTCTAACGATAGGTTGCATCTTTCTTAAGTACAAGTGTTGAATTATTAGTTAAATTCAAAAAACAAAAACAAGTTTTGTTTTCAAAACTACTTTTTTTTTTTTTTTTTTTTTGTTCTTATTAGAGAGAAGTTTATATTTCTATTCAATTACGTCTTATTATACCAATGCTATATGCTAAAAATAGCTTAGTTAACAGCATAAAACCATCAACAAACCCCACAAATCTAACCCCACAAAAATAGCCACTAACTCTAAATATATACTTGACAACTTTCCTACAAATTGGAACTTTGTACTAATCTAAAACAAGTTATAATCCAAAACAGATTGAGTTAACTAACAAGTTTAAAGGTGAAATAAGTATTTATAGGCTTAATTTTAAAAACTAAAAATAAAAAACCAACTAATTAGTAAACGGAGCCTAAACTTCTGTGTTGAGTAGTCCATTAAGAAGTACAATAGTCACACAACTGTATTGAGTATCTGATTATTGAGATTGATTACACTTTCAACTGTGTTAATTCAGGTAGGGAGACCAATTATATATGGGCTAGCTGCAAAGGGAGAAAGGGGGGTAAGAACGGTGTTGGAAATGCTGAAGAATGAGTTAGAGATTAGTATGACACTTTCCGGTTGCCCATGTATCAAAGACATTACTAGAAGCCATGTGAGGACACACACACACTATGATAAGCTTCATTCTATGCTTTGATGTATTTTCTTAGCAGCTACATAAACATTTCTGATTTACAAAATGAAGAAAGGGATGGGAGTTGTTAAATATGTAACTCCAAATTCAGATATTTGGTAACCTGTGGCTTTGTACTGTATACATGCTAAAAGCTTGGTAGCTAACTATATGTTTGCATTTATGTATTGGGAGGAGGAACAGAATGAATTAATATAATAAGAAGATTTTGTGTTAATAATGTTAAATAAA

mRNA sequence

CAAGACTTCATCTTCTAAGAGAATTATACATTAGGATAGTTGGTGTCATATTAATTTTGCTACCCAATTCCTCTGTTTTTAAAGTTCCAGCCATGAATCTGGGAAATTTCTATCTTCCCTTCCTCAAGAAGTGGATTTATATGGTTTTAGAGACTATGGCAGTATTATCCAAGATCTTGACTTTGGCTGATATTTGTTTGAATGGATTTTCATGGTTACTAAAAGCTCTAAAAAGGTATTCTTCTCTTCTTGTCTCTATTTAATCAGGATTAAAATGTCAACCGAACCAGTGAATGTGGATGAGTTCGAAGAATTGGCTAGGCAGGCACTTCCAAGAATGTATTACGATTACTATGCAGGAGGAGCTGAGGACCAGCACACACTTAGACAGAACATACAAGCTTTCTGTAGAATCACGATACAACCTCGAATTCTTGTTGACGTGAGTAAAATTGATATGTCAACAACGATCTTGGGCTATCGTATCTCTGCACCTATTATGGTTGCTCCAACCGGTGCACATAAGCTGGCATACCATGAAGGAGAGTTAGCCACAGCCAGAGCAGCTGCTGCTGCAAAAACTATAATGGTTCTATCCTTTGGTTCCTCCTACTCAATGGAGGAAGTTGCCTCTAGCTGCAATGCTATCCGTTTCTTTCAATTATATGTTTTCAAATGGCGTGATATCTCGACTATGCTAGTACAGAGAGCTGAGAGATTTGGATATAAGGCCATTGTTCTGACTGCTGATACGCCTCGACTTGGTAAAAGGGAGGCTGATATAAAGAACAAGATGATTACACCACCACAGAAGAATTTTGAAGGCTTGTTCTCTGTTAAAGTTGAAGTTGAGTCTGATCAAGGTTCGTTATTAGAGTCTTTTCTTAATGAGGCCTTTGACCCATCCCTTTGTTGGAAAGATATAGCATGGTTGAAGTCAATTACTAGCTTGCCAATTCTGATAAAGGGAATTCTCACTCATGAAGATGCAATTAAGGCTGTGGAAGTAGGTGTTGATGGAATTATTGTCTCCAATCATGGAGCTCGCCAACTAGATTTTTCTCCTGCCACCATTTCTGTTCTTGAAGAGAAAGCAAATTGCGGGTAGTTAATGCTGTGAAGGGTAAAGTTCCTGTGCTATTAGATGGAGGTGTGAGGCGAAGAACAGATGTGTTTAGGGCATTAGCCCTTGGTGCACAGGCAGTCCTTGTAAGCTCTCCAACTTTCAATTAGCCTTCTAGGGTAGGGAGACCAATTATATATGGGCTAGCTGCAAAGGGAGAAAGGGGGGTAAGAACGGTGTTGGAAATGCTGAAGAATGAGTTAGAGATTAGTATGACACTTTCCGGTTGCCCATGTATCAAAGACATTACTAGAAGCCATGTGAGGACACACACACACTATGATAAGCTTCATTCTATGCTTTGATGTATTTTCTTAGCAGCTACATAAACATTTCTGATTTACAAAATGAAGAAAGGGATGGGAGTTGTTAAATATGTAACTCCAAATTCAGATATTTGGTAACCTGTGGCTTTGTACTGTATACATGCTAAAAGCTTGGTAGCTAACTATATGTTTGCATTTATGTATTGGGAGGAGGAACAGAATGAATTAATATAATAAGAAGATTTTGTGTTAATAATGTTAAATAAA

Coding sequence (CDS)

ATGGTTACTAAAAGCTCTAAAAAGGTATTCTTCTCTTCTTGTCTCTATTTAATCAGGATTAAAATGTCAACCGAACCAGTGAATGTGGATGAGTTCGAAGAATTGGCTAGGCAGGCACTTCCAAGAATGTATTACGATTACTATGCAGGAGGAGCTGAGGACCAGCACACACTTAGACAGAACATACAAGCTTTCTGTAGAATCACGATACAACCTCGAATTCTTGTTGACGTGAGTAAAATTGATATGTCAACAACGATCTTGGGCTATCGTATCTCTGCACCTATTATGGTTGCTCCAACCGGTGCACATAAGCTGGCATACCATGAAGGAGAGTTAGCCACAGCCAGAGCAGCTGCTGCTGCAAAAACTATAATGGTTCTATCCTTTGGTTCCTCCTACTCAATGGAGGAAGTTGCCTCTAGCTGCAATGCTATCCGTTTCTTTCAATTATATGTTTTCAAATGGCGTGATATCTCGACTATGCTAGTACAGAGAGCTGAGAGATTTGGATATAAGGCCATTGTTCTGACTGCTGATACGCCTCGACTTGGTAAAAGGGAGGCTGATATAAAGAACAAGATGATTACACCACCACAGAAGAATTTTGAAGGCTTGTTCTCTGTTAAAGTTGAAGTTGAGTCTGATCAAGGTTCGTTATTAGAGTCTTTTCTTAATGAGGCCTTTGACCCATCCCTTTGTTGGAAAGATATAGCATGGTTGAAGTCAATTACTAGCTTGCCAATTCTGATAAAGGGAATTCTCACTCATGAAGATGCAATTAAGGCTGTGGAAGTAGGTGTTGATGGAATTATTGTCTCCAATCATGGAGCTCGCCAACTAGATTTTTCTCCTGCCACCATTTCTGTTCTTGAAGAGAAAGCAAATTGCGGGTAG

Protein sequence

MVTKSSKKVFFSSCLYLIRIKMSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQKNFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAIKAVEVGVDGIIVSNHGARQLDFSPATISVLEEKANCG

Homology

[Close]

BLAST of PI0005459 vs. ExPASy Swiss-Prot
Match: Q24JJ8 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=GLO3 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 7.5e-105
Identity = 190/267 (71.16%), Postives = 226/267 (84.64%), Query Frame = 0


Query: 27  VNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMSTT 86
           VNVDEF+ELA+QALP+MYYD+Y GGAEDQHTL +N+QAF RI  +PR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 87  ILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCNAI 146
           ILGY ISAPIM+APTG HKLA+ EGE ATA+AAAA  TIM++S+ SS + EE+ASSCNA+
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 147 RFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQKNFEGL 206
           RF Q+YV+K RDI+  +V+RAE+ G+KAIVLT D PRLG+READIKNKMI+P  KNFEGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 207 FSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAIKAVEV 266
           FS   EV   +GS +++F + AFD S  WKDI WL+SIT LPIL+KGILT EDA+KAVE 
Sbjct: 185 FS--TEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEA 244

Query: 267 GVDGIIVSNHGARQLDFSPATISVLEE 294
           GVDGIIVSNHG RQLD+SPATI+VLEE
Sbjct: 245 GVDGIIVSNHGGRQLDYSPATITVLEE 269

[Close]

BLAST of PI0005459 vs. ExPASy Swiss-Prot
Match: Q9LJH5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=GLO4 PE=2 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 3.7e-104
Identity = 186/267 (69.66%), Postives = 229/267 (85.77%), Query Frame = 0


Query: 27  VNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMSTT 86
           VNVDEF+ELA+QALP+MYYD+Y GGAEDQHTL +N+QAF RI  +PR+LVDVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 87  ILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCNAI 146
           +LGY ISAPIM+APT  HKLA+ +GE+ATA+AAAA  TIM++SF S+ ++EEVASSCNA+
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 147 RFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQKNFEGL 206
           RF Q+YV+K RD++  +V+RAE+ G+KAIVLT D PRLG+READIKNKMI+P  KNFEGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 207 FSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAIKAVEV 266
             V  EV  ++GS +E+F + AFD SL WKDI WL+SIT LPIL+KG+LT EDA+KAVE 
Sbjct: 185 --VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEA 244

Query: 267 GVDGIIVSNHGARQLDFSPATISVLEE 294
           GVDGI+VSNHGARQLD+SPATI+VLEE
Sbjct: 245 GVDGIVVSNHGARQLDYSPATITVLEE 269

[Close]

BLAST of PI0005459 vs. ExPASy Swiss-Prot
Match: B8B8K5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=39946 GN=GLO4 PE=3 SV=2)

HSP 1 Score: 349.0 bits (894), Expect = 5.4e-95
Identity = 174/268 (64.93%), Postives = 217/268 (80.97%), Query Frame = 0


Query: 26  PVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMST 85
           PVNV E++ELA++ALP+M YDY  GGAED+HTLR+NI A+ RI ++PR+LVDVSKIDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 86  TILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCNA 145
           T+LGY + +PI+VAPTG HKLA+ EGE ATARAAA+   IMVLSF SS  +E+VASSCNA
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 146 IRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQKNFEG 205
           IRF+QLYV+K R++S  LV+RAE  G+KA++LT DTP LG+READI+NKM+ P   N EG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 206 LFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAIKAVE 265
           L ++  + ++  GS LE F     DPSL WKDI WLKSITS+PI +KGI+T EDA +AVE
Sbjct: 186 LMTID-DHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVE 245

Query: 266 VGVDGIIVSNHGARQLDFSPATISVLEE 294
            GV G+IVSNHGARQLD++PATI+ LEE
Sbjct: 246 AGVAGVIVSNHGARQLDYAPATIAALEE 272

[Close]

BLAST of PI0005459 vs. ExPASy Swiss-Prot
Match: Q8H3I4 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLO4 PE=1 SV=2)

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-94
Identity = 174/268 (64.93%), Postives = 216/268 (80.60%), Query Frame = 0


Query: 26  PVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMST 85
           PVNV E++ELA++ALP+M YDY  GGAED+HTLR+NI A+ RI ++PR+LVDVSKIDMST
Sbjct: 6   PVNVREYQELAKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMST 65

Query: 86  TILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCNA 145
           T+LGY + +PI+VAPTG HKLA+ EGE ATARAAA+   IMVLSF SS  +E+VASSCNA
Sbjct: 66  TLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSCNA 125

Query: 146 IRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQKNFEG 205
           IRF+QLYV+K R++S  LV+RAE  G+KA++LT DTP LG+READI+NKM+ P   N EG
Sbjct: 126 IRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEG 185

Query: 206 LFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAIKAVE 265
           L +   + ++  GS LE F     DPSL WKDI WLKSITS+PI +KGI+T EDA +AVE
Sbjct: 186 LMTTD-DHDTTNGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVE 245

Query: 266 VGVDGIIVSNHGARQLDFSPATISVLEE 294
            GV G+IVSNHGARQLD++PATI+ LEE
Sbjct: 246 AGVAGVIVSNHGARQLDYAPATIAALEE 272

[Close]

BLAST of PI0005459 vs. ExPASy Swiss-Prot
Match: P05414 (Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.9e-84
Identity = 165/272 (60.66%), Postives = 207/272 (76.10%), Query Frame = 0


Query: 25  EPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMS 84
           E  NV+E+E +A+Q LP+M YDYYA GAEDQ TL +N  AF RI  +PRIL+DV+ IDM+
Sbjct: 2   EITNVNEYEAIAKQKLPKMVYDYYASGAEDQWTLAENRNAFSRILFRPRILIDVTNIDMT 61

Query: 85  TTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCN 144
           TTILG++IS PIM+APT   K+A+ EGE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTILGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 145 AIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPP---QK 204
            IRFFQLYV+K R++   LV+RAER G+KAI LT DTPRLG+READIKN+ + PP    K
Sbjct: 122 GIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLK 181

Query: 205 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 264
           NFEG+   K++  +D G  L S++    D SL WKD+AWL++ITSLPIL+KG++T EDA 
Sbjct: 182 NFEGIDLGKMDKANDSG--LSSYVAGQIDRSLSWKDVAWLQTITSLPILVKGVITAEDAR 241

Query: 265 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
            AV+ G  GIIVSNHGARQLD+ PATI  LEE
Sbjct: 242 LAVQHGAAGIIVSNHGARQLDYVPATIMALEE 271

[Close]

BLAST of PI0005459 vs. ExPASy TrEMBL
Match: A0A1S4E3I3 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499256 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.0e-141
Identity = 258/272 (94.85%), Postives = 265/272 (97.43%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMSTTILGYRISAPIMVAPTG+HKLAYHEGELATARAAAAAKTIMVLS  SSYSMEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTGSHKLAYHEGELATARAAAAAKTIMVLSLASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RD+ST+LVQRAERFGYKAIVLTADTPRLG+READIKNKMI PPQK
Sbjct: 121 SCNAIRFFQLYVFKQRDVSTLLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+NEAFDPSLCWKD+AWLKSITSLPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNEAFDPSLCWKDVAWLKSITSLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHGARQLDFSPAT+S LEE
Sbjct: 241 KAVEVGVDGIIVSNHGARQLDFSPATVSALEE 272

[Close]

BLAST of PI0005459 vs. ExPASy TrEMBL
Match: A0A5D3D6K0 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00310 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.0e-141
Identity = 258/272 (94.85%), Postives = 265/272 (97.43%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMSTTILGYRISAPIMVAPTG+HKLAYHEGELATARAAAAAKTIMVLS  SSYSMEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTGSHKLAYHEGELATARAAAAAKTIMVLSLASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RD+ST+LVQRAERFGYKAIVLTADTPRLG+READIKNKMI PPQK
Sbjct: 121 SCNAIRFFQLYVFKQRDVSTLLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+NEAFDPSLCWKD+AWLKSITSLPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNEAFDPSLCWKDVAWLKSITSLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHGARQLDFSPAT+S LEE
Sbjct: 241 KAVEVGVDGIIVSNHGARQLDFSPATVSALEE 272

[Close]

BLAST of PI0005459 vs. ExPASy TrEMBL
Match: A0A1S3CCY6 ((S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499256 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.0e-141
Identity = 258/272 (94.85%), Postives = 265/272 (97.43%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMSTTILGYRISAPIMVAPTG+HKLAYHEGELATARAAAAAKTIMVLS  SSYSMEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTGSHKLAYHEGELATARAAAAAKTIMVLSLASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RD+ST+LVQRAERFGYKAIVLTADTPRLG+READIKNKMI PPQK
Sbjct: 121 SCNAIRFFQLYVFKQRDVSTLLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+NEAFDPSLCWKD+AWLKSITSLPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNEAFDPSLCWKDVAWLKSITSLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHGARQLDFSPAT+S LEE
Sbjct: 241 KAVEVGVDGIIVSNHGARQLDFSPATVSALEE 272

[Close]

BLAST of PI0005459 vs. ExPASy TrEMBL
Match: A0A0A0LBZ1 ((S)-2-hydroxy-acid oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G645970 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 6.3e-139
Identity = 257/272 (94.49%), Postives = 261/272 (95.96%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTL QNIQAFCRITIQPRILVDVS+I
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLTQNIQAFCRITIQPRILVDVSEI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMST ILGYRISAPIM+APTGAHKLAYHEGELATARAAAAAKTIMVLSF SSYSMEEVAS
Sbjct: 61  DMSTKILGYRISAPIMIAPTGAHKLAYHEGELATARAAAAAKTIMVLSFASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RDISTMLVQRAER GYKAIVLTADTPRLG+READIKNKMITPPQK
Sbjct: 121 SCNAIRFFQLYVFKRRDISTMLVQRAERLGYKAIVLTADTPRLGRREADIKNKMITPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+N AFDPSLCWKDIAWLKSIT LPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNGAFDPSLCWKDIAWLKSITCLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHG RQLDFSPATIS LEE
Sbjct: 241 KAVEVGVDGIIVSNHGGRQLDFSPATISALEE 272

[Close]

BLAST of PI0005459 vs. ExPASy TrEMBL
Match: A0A6J1D4P5 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.3e-125
Identity = 235/273 (86.08%), Postives = 256/273 (93.77%), Query Frame = 0


Query: 21  KMSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSK 80
           KMS+EPVNV++++ELARQALP+MYYDYYAGGAEDQHTLR+N+QAF RI I+PRILVDVSK
Sbjct: 9   KMSSEPVNVNDYKELARQALPKMYYDYYAGGAEDQHTLRENMQAFHRIIIRPRILVDVSK 68

Query: 81  IDMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVA 140
           IDMSTTILGY IS PIMVAPTGAHKLA+ EGELATARAAAAAKTIMVLS+ SSYS+EEVA
Sbjct: 69  IDMSTTILGYLISEPIMVAPTGAHKLAHCEGELATARAAAAAKTIMVLSYASSYSIEEVA 128

Query: 141 SSCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQ 200
           SSCNA+RFFQLYVF+ RDIS +LVQRAER GYKAIVLTADTPRLG+READIKNKMITPP 
Sbjct: 129 SSCNAVRFFQLYVFRKRDISALLVQRAERSGYKAIVLTADTPRLGRREADIKNKMITPPL 188

Query: 201 KNFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDA 260
           KN EGL S  VEV SD+GS+LESF+NEAFD SLCW+DI WLKSITSLPILIKGILTHEDA
Sbjct: 189 KNLEGLLS--VEVGSDKGSILESFVNEAFDASLCWEDIGWLKSITSLPILIKGILTHEDA 248

Query: 261 IKAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           IKAVEVGVDGIIVSNHGARQLDFSPAT+SVLEE
Sbjct: 249 IKAVEVGVDGIIVSNHGARQLDFSPATVSVLEE 279

[Close]

BLAST of PI0005459 vs. NCBI nr
Match: XP_016902540.1 (PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Cucumis melo] >XP_016902541.1 PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Cucumis melo])

HSP 1 Score: 511.1 bits (1315), Expect = 6.2e-141
Identity = 258/272 (94.85%), Postives = 265/272 (97.43%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMSTTILGYRISAPIMVAPTG+HKLAYHEGELATARAAAAAKTIMVLS  SSYSMEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTGSHKLAYHEGELATARAAAAAKTIMVLSLASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RD+ST+LVQRAERFGYKAIVLTADTPRLG+READIKNKMI PPQK
Sbjct: 121 SCNAIRFFQLYVFKQRDVSTLLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+NEAFDPSLCWKD+AWLKSITSLPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNEAFDPSLCWKDVAWLKSITSLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHGARQLDFSPAT+S LEE
Sbjct: 241 KAVEVGVDGIIVSNHGARQLDFSPATVSALEE 272

[Close]

BLAST of PI0005459 vs. NCBI nr
Match: XP_008460443.1 (PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucumis melo] >KAA0053518.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucumis melo var. makuwa] >TYK19113.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 511.1 bits (1315), Expect = 6.2e-141
Identity = 258/272 (94.85%), Postives = 265/272 (97.43%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMSTTILGYRISAPIMVAPTG+HKLAYHEGELATARAAAAAKTIMVLS  SSYSMEEVAS
Sbjct: 61  DMSTTILGYRISAPIMVAPTGSHKLAYHEGELATARAAAAAKTIMVLSLASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RD+ST+LVQRAERFGYKAIVLTADTPRLG+READIKNKMI PPQK
Sbjct: 121 SCNAIRFFQLYVFKQRDVSTLLVQRAERFGYKAIVLTADTPRLGRREADIKNKMIAPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+NEAFDPSLCWKD+AWLKSITSLPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNEAFDPSLCWKDVAWLKSITSLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHGARQLDFSPAT+S LEE
Sbjct: 241 KAVEVGVDGIIVSNHGARQLDFSPATVSALEE 272

[Close]

BLAST of PI0005459 vs. NCBI nr
Match: KAE8650883.1 (hypothetical protein Csa_002366 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 1.3e-138
Identity = 257/272 (94.49%), Postives = 261/272 (95.96%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTL QNIQAFCRITIQPRILVDVS+I
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLTQNIQAFCRITIQPRILVDVSEI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMST ILGYRISAPIM+APTGAHKLAYHEGELATARAAAAAKTIMVLSF SSYSMEEVAS
Sbjct: 61  DMSTKILGYRISAPIMIAPTGAHKLAYHEGELATARAAAAAKTIMVLSFASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RDISTMLVQRAER GYKAIVLTADTPRLG+READIKNKMITPPQK
Sbjct: 121 SCNAIRFFQLYVFKRRDISTMLVQRAERLGYKAIVLTADTPRLGRREADIKNKMITPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+N AFDPSLCWKDIAWLKSIT LPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNGAFDPSLCWKDIAWLKSITCLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHG RQLDFSPATIS LEE
Sbjct: 241 KAVEVGVDGIIVSNHGGRQLDFSPATISALEE 272

[Close]

BLAST of PI0005459 vs. NCBI nr
Match: XP_004144436.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X1 [Cucumis sativus] >XP_011651715.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X1 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 1.3e-138
Identity = 257/272 (94.49%), Postives = 261/272 (95.96%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTL QNIQAFCRITIQPRILVDVS+I
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLTQNIQAFCRITIQPRILVDVSEI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMST ILGYRISAPIM+APTGAHKLAYHEGELATARAAAAAKTIMVLSF SSYSMEEVAS
Sbjct: 61  DMSTKILGYRISAPIMIAPTGAHKLAYHEGELATARAAAAAKTIMVLSFASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RDISTMLVQRAER GYKAIVLTADTPRLG+READIKNKMITPPQK
Sbjct: 121 SCNAIRFFQLYVFKRRDISTMLVQRAERLGYKAIVLTADTPRLGRREADIKNKMITPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+N AFDPSLCWKDIAWLKSIT LPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNGAFDPSLCWKDIAWLKSITCLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHG RQLDFSPATIS LEE
Sbjct: 241 KAVEVGVDGIIVSNHGGRQLDFSPATISALEE 272

[Close]

BLAST of PI0005459 vs. NCBI nr
Match: XP_031738603.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X2 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 1.3e-138
Identity = 257/272 (94.49%), Postives = 261/272 (95.96%), Query Frame = 0


Query: 22  MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKI 81
           MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTL QNIQAFCRITIQPRILVDVS+I
Sbjct: 1   MSTEPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLTQNIQAFCRITIQPRILVDVSEI 60

Query: 82  DMSTTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVAS 141
           DMST ILGYRISAPIM+APTGAHKLAYHEGELATARAAAAAKTIMVLSF SSYSMEEVAS
Sbjct: 61  DMSTKILGYRISAPIMIAPTGAHKLAYHEGELATARAAAAAKTIMVLSFASSYSMEEVAS 120

Query: 142 SCNAIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQK 201
           SCNAIRFFQLYVFK RDISTMLVQRAER GYKAIVLTADTPRLG+READIKNKMITPPQK
Sbjct: 121 SCNAIRFFQLYVFKRRDISTMLVQRAERLGYKAIVLTADTPRLGRREADIKNKMITPPQK 180

Query: 202 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 261
           N EGL SVKVEVESDQGSLLESF+N AFDPSLCWKDIAWLKSIT LPILIKGILTHEDAI
Sbjct: 181 NLEGLLSVKVEVESDQGSLLESFVNGAFDPSLCWKDIAWLKSITCLPILIKGILTHEDAI 240

Query: 262 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
           KAVEVGVDGIIVSNHG RQLDFSPATIS LEE
Sbjct: 241 KAVEVGVDGIIVSNHGGRQLDFSPATISALEE 272

[Close]

BLAST of PI0005459 vs. TAIR 10
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 381.7 bits (979), Expect = 5.3e-106
Identity = 190/267 (71.16%), Postives = 226/267 (84.64%), Query Frame = 0


Query: 27  VNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMSTT 86
           VNVDEF+ELA+QALP+MYYD+Y GGAEDQHTL +N+QAF RI  +PR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 87  ILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCNAI 146
           ILGY ISAPIM+APTG HKLA+ EGE ATA+AAAA  TIM++S+ SS + EE+ASSCNA+
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 147 RFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQKNFEGL 206
           RF Q+YV+K RDI+  +V+RAE+ G+KAIVLT D PRLG+READIKNKMI+P  KNFEGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 207 FSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAIKAVEV 266
           FS   EV   +GS +++F + AFD S  WKDI WL+SIT LPIL+KGILT EDA+KAVE 
Sbjct: 185 FS--TEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEA 244

Query: 267 GVDGIIVSNHGARQLDFSPATISVLEE 294
           GVDGIIVSNHG RQLD+SPATI+VLEE
Sbjct: 245 GVDGIIVSNHGGRQLDYSPATITVLEE 269

[Close]

BLAST of PI0005459 vs. TAIR 10
Match: AT3G14150.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 381.7 bits (979), Expect = 5.3e-106
Identity = 190/267 (71.16%), Postives = 226/267 (84.64%), Query Frame = 0


Query: 27  VNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMSTT 86
           VNVDEF+ELA+QALP+MYYD+Y GGAEDQHTL +N+QAF RI  +PR+LVDVSKIDMST 
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSKIDMSTK 64

Query: 87  ILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCNAI 146
           ILGY ISAPIM+APTG HKLA+ EGE ATA+AAAA  TIM++S+ SS + EE+ASSCNA+
Sbjct: 65  ILGYPISAPIMIAPTGNHKLAHPEGETATAKAAAACNTIMIVSYMSSCTFEEIASSCNAV 124

Query: 147 RFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQKNFEGL 206
           RF Q+YV+K RDI+  +V+RAE+ G+KAIVLT D PRLG+READIKNKMI+P  KNFEGL
Sbjct: 125 RFLQIYVYKRRDITAQVVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 207 FSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAIKAVEV 266
           FS   EV   +GS +++F + AFD S  WKDI WL+SIT LPIL+KGILT EDA+KAVE 
Sbjct: 185 FS--TEVRPSKGSGVQAFASRAFDASFSWKDIEWLRSITELPILVKGILTREDALKAVEA 244

Query: 267 GVDGIIVSNHGARQLDFSPATISVLEE 294
           GVDGIIVSNHG RQLD+SPATI+VLEE
Sbjct: 245 GVDGIIVSNHGGRQLDYSPATITVLEE 269

[Close]

BLAST of PI0005459 vs. TAIR 10
Match: AT3G14130.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 379.4 bits (973), Expect = 2.6e-105
Identity = 186/267 (69.66%), Postives = 229/267 (85.77%), Query Frame = 0


Query: 27  VNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMSTT 86
           VNVDEF+ELA+QALP+MYYD+Y GGAEDQHTL +N+QAF RI  +PR+LVDVS IDMST+
Sbjct: 5   VNVDEFQELAKQALPKMYYDFYNGGAEDQHTLNENVQAFRRIMFRPRVLVDVSNIDMSTS 64

Query: 87  ILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCNAI 146
           +LGY ISAPIM+APT  HKLA+ +GE+ATA+AAAA  TIM++SF S+ ++EEVASSCNA+
Sbjct: 65  MLGYPISAPIMIAPTAMHKLAHPKGEIATAKAAAACNTIMIVSFMSTCTIEEVASSCNAV 124

Query: 147 RFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQKNFEGL 206
           RF Q+YV+K RD++  +V+RAE+ G+KAIVLT D PRLG+READIKNKMI+P  KNFEGL
Sbjct: 125 RFLQIYVYKRRDVTAQIVKRAEKAGFKAIVLTVDVPRLGRREADIKNKMISPQLKNFEGL 184

Query: 207 FSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAIKAVEV 266
             V  EV  ++GS +E+F + AFD SL WKDI WL+SIT LPIL+KG+LT EDA+KAVE 
Sbjct: 185 --VSTEVRPNEGSGVEAFASSAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEA 244

Query: 267 GVDGIIVSNHGARQLDFSPATISVLEE 294
           GVDGI+VSNHGARQLD+SPATI+VLEE
Sbjct: 245 GVDGIVVSNHGARQLDYSPATITVLEE 269

[Close]

BLAST of PI0005459 vs. TAIR 10
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 311.6 bits (797), Expect = 6.8e-85
Identity = 163/272 (59.93%), Postives = 206/272 (75.74%), Query Frame = 0


Query: 25  EPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMS 84
           E  NV E++ +A+Q LP+M YDYYA GAEDQ TL++N  AF RI  +PRIL+DVSKIDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 85  TTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCN 144
           TT+LG++IS PIMVAPT   K+A+ +GE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 145 AIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQ---K 204
            IRFFQLYV+K R++   LV+RAER G+KAI LT DTPRLG+RE+DIKN+   PP    K
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 205 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 264
           NFEGL   K++  +D G  L S++    D +L WKD+ WL++IT LPIL+KG+LT EDA 
Sbjct: 182 NFEGLDLGKMDEANDSG--LASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDAR 241

Query: 265 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
            A++ G  GIIVSNHGARQLD+ PATIS LEE
Sbjct: 242 IAIQAGAAGIIVSNHGARQLDYVPATISALEE 271

[Close]

BLAST of PI0005459 vs. TAIR 10
Match: AT3G14420.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 311.6 bits (797), Expect = 6.8e-85
Identity = 163/272 (59.93%), Postives = 206/272 (75.74%), Query Frame = 0


Query: 25  EPVNVDEFEELARQALPRMYYDYYAGGAEDQHTLRQNIQAFCRITIQPRILVDVSKIDMS 84
           E  NV E++ +A+Q LP+M YDYYA GAEDQ TL++N  AF RI  +PRIL+DVSKIDM+
Sbjct: 2   EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMT 61

Query: 85  TTILGYRISAPIMVAPTGAHKLAYHEGELATARAAAAAKTIMVLSFGSSYSMEEVASSCN 144
           TT+LG++IS PIMVAPT   K+A+ +GE ATARAA+AA TIM LS  ++ S+EEVAS+  
Sbjct: 62  TTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 121

Query: 145 AIRFFQLYVFKWRDISTMLVQRAERFGYKAIVLTADTPRLGKREADIKNKMITPPQ---K 204
            IRFFQLYV+K R++   LV+RAER G+KAI LT DTPRLG+RE+DIKN+   PP    K
Sbjct: 122 GIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLK 181

Query: 205 NFEGLFSVKVEVESDQGSLLESFLNEAFDPSLCWKDIAWLKSITSLPILIKGILTHEDAI 264
           NFEGL   K++  +D G  L S++    D +L WKD+ WL++IT LPIL+KG+LT EDA 
Sbjct: 182 NFEGLDLGKMDEANDSG--LASYVAGQIDRTLSWKDVQWLQTITKLPILVKGVLTGEDAR 241

Query: 265 KAVEVGVDGIIVSNHGARQLDFSPATISVLEE 294
            A++ G  GIIVSNHGARQLD+ PATIS LEE
Sbjct: 242 IAIQAGAAGIIVSNHGARQLDYVPATISALEE 271

The following BLAST results are available for this feature:

BLAST of PI0005459 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q24JJ8	7.5e-105	71.16	Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LJH5	3.7e-104	69.66	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
B8B8K5	5.4e-95	64.93	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=399...	[more]
Q8H3I4	1.2e-94	64.93	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=3...	[more]
P05414	1.9e-84	60.66	Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea OX=3562 PE=1 SV=1	[more]

BLAST of PI0005459 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E3I3	3.0e-141	94.85	(S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499256 PE=3 SV=1	[more]
A0A5D3D6K0	3.0e-141	94.85	(S)-2-hydroxy-acid oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3CCY6	3.0e-141	94.85	(S)-2-hydroxy-acid oxidase OS=Cucumis melo OX=3656 GN=LOC103499256 PE=3 SV=1	[more]
A0A0A0LBZ1	6.3e-139	94.49	(S)-2-hydroxy-acid oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G645970 PE=3 SV=1	[more]
A0A6J1D4P5	2.3e-125	86.08	(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016934 PE=3 S...	[more]

BLAST of PI0005459 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016902540.1	6.2e-141	94.85	PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X1 [Cucumis ...	[more]
XP_008460443.1	6.2e-141	94.85	PREDICTED: peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucumis ...	[more]
KAE8650883.1	1.3e-138	94.49	hypothetical protein Csa_002366 [Cucumis sativus]	[more]
XP_004144436.1	1.3e-138	94.49	peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X1 [Cucumis sativus] >XP_011...	[more]
XP_031738603.1	1.3e-138	94.49	peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X2 [Cucumis sativus]	[more]

BLAST of PI0005459 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14150.1	5.3e-106	71.16	Aldolase-type TIM barrel family protein	[more]
AT3G14150.2	5.3e-106	71.16	Aldolase-type TIM barrel family protein	[more]
AT3G14130.1	2.6e-105	69.66	Aldolase-type TIM barrel family protein	[more]
AT3G14420.1	6.8e-85	59.93	Aldolase-type TIM barrel family protein	[more]
AT3G14420.2	6.8e-85	59.93	Aldolase-type TIM barrel family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 24..297 e-value: 3.0E-112 score: 377.0
IPR000262	FMN-dependent dehydrogenase	PFAM	PF01070	FMN_dh	coord: 36..294 e-value: 1.0E-91 score: 307.6
None	No IPR available	PIRSR	PIRSR000138-2	PIRSR000138-2	coord: 22..293 e-value: 9.8E-94 score: 312.1
None	No IPR available	PANTHER	PTHR10578:SF116	PEROXISOMAL (S)-2-HYDROXY-ACID OXIDASE GLO4-LIKE	coord: 22..293
None	No IPR available	PANTHER	PTHR10578	S -2-HYDROXY-ACID OXIDASE-RELATED	coord: 22..293
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 26..294
IPR008259	FMN-dependent alpha-hydroxy acid dehydrogenase, active site	PROSITE	PS00557	FMN_HYDROXY_ACID_DH_1	coord: 274..280
IPR037396	FMN hydroxy acid dehydrogenase domain	PROSITE	PS51349	FMN_HYDROXY_ACID_DH_2	coord: 21..298 score: 82.582047
IPR012133	Alpha-hydroxy acid dehydrogenase, FMN-dependent	CDD	cd02809	alpha_hydroxyacid_oxid_FMN	coord: 30..295 e-value: 1.60804E-103 score: 302.444

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0005459.1	PI0005459.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009854	oxidative photosynthetic carbon pathway
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0003973	(S)-2-hydroxy-acid oxidase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity