PI0005421 (gene) Melon (PI 482460) v1

Overview
NamePI0005421
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionNAD(P)-bd_dom domain-containing protein
Locationchr02: 5236719 .. 5241461 (+)
RNA-Seq ExpressionPI0005421
SyntenyPI0005421
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCATTACCAATCTCTCTCTCTCATACATCCCTCATTTTCCTCATCCACCAACAAATTTTCACAAAACATTCCAAACTCTTCCTCTCTTCGCCCTTCCGCCGCTGTGTTCATCTTCATCGTTCGAGAATCAACTTCGCAAGAGCATTTCAAGCTGCAGATTACGATCCCCAGCTATTCGTGCAGTCAATCAAGAAGTTGTTCAGACTCCAAGTTCAGAATCGACCCGTGACTCGAAAACTGGTCCTGTTGCTTCTTCTAAACTCGTCCTTGTTGTCGGAGGCTCCGGCGGCGTTGGTAGGTTCTATCTGTATTATTCTCTGTATCTTTCCGTTGAATATATATGCACGTACATGATAAATTGAGTAGCTTAATTGAATCAGGGAGTGTTAACTAGTTGTTTTTATGTAAAATTGAGTGTGTTGGTTGTAGTTCTTAATGGAATAGGTACTGGTAATTTTAATGAAGTTTTTAGTTCTTAGTCTGACTAATTAATAAGCTATATTGGAGGGCTTCTTTTGTCCACTGTGTGATTTATGTTCTTGCTTTGATGTTTCACTTTCTTAAATAGGACAGTTGGTGGCAGCAGCTTTGCTTGACCGGAATGTCAAATTACGCCTCTTACTGCGTGATCCGGAGAAAGCAAATACATTATTTGGTGAACAAGATGAAAATTTGTTACAGGTAATAAGGTCTGTCTTAGTCAAAGCAAGGTCGTGGTACAGAATGTCAGAAGCTTATGATTTCATGTTTCATTTCGCTTGGTTGAGTGTAGCACATGAGAGGACTTGGGAGCTTTTAGGTTATTGTTGTTGAACTTTGAAGCAGAGATCAGTGTGGCATGATCAGTTGACTGGATTCAATCTTGATTACTAATTTACTGTTGATGTGATTAGTAACAAATTAGAATGTTTTCTTCAATTCATTGCACTATGTAGGCTGGAGGATATGTTGGTTCTTAAAAGTTTCATCACATTCTCATGTAGAGTTTGTTGTTACATAGAGTTTTAACTTTGACAGGTATGCAAAGGCGACACAAGGAACCCAGAAGACCTAGATCCATCTATATTTGAGGTTTGTGATCCAGATTAGATTACACTACCTTACCATTCTTTTTTTGTTTCAAATTCTTTAAGGATGCTTTTATGATAGGACACTTCCTGAGTTCATTTGATTATTCTTGGGCACCCTGAGGCTTTCCCACAGCAGCACCAAATAAAATTATCAGTTATTAATTGCTTGCAGTCCTTTTATATACAAAAATAACCTTGCACGAAATAGTACTCTTTATTCACTTCCCCAATCCCCAGTTCAAGAGAAATGTAACAGAGGGGAGCGAGAGGAGTGGTTGTGTTAAATATCCTTTTGCAAGGACAGAGTAGAGGTGAATGGGAGAGATGAGGTGACAGTTGATGGTGTAGTGTTGGGTACTAGAAAGTTATTCAATGGATGGCCTAATTAATAGGTTCTTCATGCGCAAGATTTGAGATCAATTCCCTCTTGCCTCCTTTTGCTTTGACTAGTAATTAAATGTGTGAGTGCAATGAAGATGGAAAATTAGAAAGACAAAAAAAAAAAAAAAAGAGAAGCAAGAAACTGAAAATTACAATATAAATCAGATTGAAAAATTTGTGATCCTAATAATATACCGTACAATTTAAGAGTTTCTCTTGCACTCTCATGAAAGGTAGAGTTGGGCTATGAATTATGATCTCAGACTTAGGCAAACTAGTCTGATCCCTCATCACAAAACTTCAATTTTAGACCTTTTGATAACTTAGATTGTTATTATTACTTTTCTTATCAGATTCCTTCTTGAGTTAGATTGTATTAGAATTATTAGTCCTGGTTTTGGATCATAATTGTTTCTTGATTTTAGTTTTTTTATAGAAGAAATGAATATTGATAGTTATAATTTTTATTGTTGTTAAAAAAACGTTATAGCTTGGTGGTGTTGTATCAGTTGTTTACAATTTTAAAATATCCGTTTTGATTTATCTGATATTCATCTGTTAGGGTGTCACGCATGTGATTTGCTGCACAGGAACGACAGCCTTTCCTTCAAGGCGATGGGATGGGGACAACACTCCTGAAAGAGTAGGTTAGAATTACACTGTTAATATCAGATGTTACGTCTTAGTGTTTTGGCTAGAAATTTAAGCCTACAGATCATTGGCACATCTCTGTCTCATTTTTCAACAACATTGTTGAAATTAATAATTAATATAACATCAGAGTTTTTTGGCCATACTCAAGATTAAAGTTGTTACTCATAAGTCATAAGCACATCAACACAGAATCAATTAAGCCTATGCTTCTCAGCTCTTCCTGACTATGGCCTTATAATTGTAGATTGGGAGGGTGTGAGAAACCTCATATCAGCAATTCCACGATCAGTAAAGAGAGTAGTTCTTGTTTCATCGATAGGGGTAACCAAATTCAATGAATTGCCGTGGAGGTAGGCCAGCTTATTCTGTAAAGCAAGCATTACATGGTAGAGGAATGAAGAAACCTTTTAAATATCGTCAATTTTTCACTAACATGAATGATTCTTCTTGAAAATAGTAATGATTGATGTGTCTGCTAAAAAGCGATTGCTTCTACAAAGCATAGCCTGCATTATTGGAACTACTGGTAGAGATCATTTTTAGTTATATCTTGTATAAACACAGACTCAAAGGATGTGTTCTTCAGTTTGTCAAACTAAGTGTCTGTCTGGTGTTATGCGCTGGTTTGGGTTTATACAGATCTGAAAAACTTTCTTAACCTTTCTTCTTAAAAGTCAGTATTCAGTTTTAACTTGCCAGAAAAAAAAAGTTCGTCAAACTGATGTTTACTTCTTCAGAATGTTCAATTATATTAACATCTGGTGTCATATAGTCTTAATGCCAAAAGGACAGTAAGACTGTTAATCATGCAGCAGAAACTTCTGCTGGTTGATTATTTATATTGCTCAGCCTGAGGCTTGACATTAAGTCGTTGATTTTCCTTTTATTGTTATTGTTTCTAAAACTTGGTATAGGAAGCAGACGAGACTGTGTCTGCATTTGTTTTGTCGAATTTAACATTTAAAAGAAAACATGTACAATATACAAACTATGCTTTCATCATAATTGGTTTCTTTTCTTTTCCTAGCATTATGAATCTTTTTGGCGTTCTCAAATACAAAAAGCAAGGGGAGGATTTTCTTCGAGACTCAGGACTTCCGTTCACAATTATCAGGTCTGTGTAGTTGGCATGAACTTGTTTTCCTAAGCTAATCGTTTTTCTTTTTCTTTCTAGAAATTGAACATAGTTTCTCCCCATTGAGCGTACTCTCACTCTTCCATGTACAAATGTGTGTTCCAGAGCTGGTAGGTTGACTGATGGACCATACACCTCGTATGATCTTAATACTTTGCTCAAAGCTACAGCAGGGCAGCGGCGTGCCGTTCTCCTTGGTCAAGGTACAACATTATAAGAATTTATAAAAATCACAAATGTTGCATTTCTATAGAATTGTGGTATGAAGAACACGTGGGGCTATCTCTGGGTGCGGTTTCATTATACATAGTTTTAGTTACCAATTATAAATCCTATGCCCTCAATTTTATGCTTTTCCTTTGATTCTATGTTAAATTACAAATTTGGTTCCTATAATATAGAGAAAGTGAAAGTTTAGTCTCTATAGTTTTAAAAGTTAAAACATAGTCTCTGACTCTCTATGGTTTGATAAAACTTCATAAATAGTCTCATGGGTTGATAAAATCCTAAAAAATAGTTCCTACTATAGAGACATTTTAGGAAGGTTTACTAAATCATAGGAACTAAATTTGCAATTTAACCTTGATTCTATTTGCTTGCAAAGAAAACAAAATTATCTAAAAAGTTTTAAAGTGAGTAGCCAGGCATGAAAATGTACAAACATATATGCAACTTCTTAGTACACCTTCCCGTGGCCATTAGCTTGTTTCCATTTCCCATCATTATAGTCTTATTCCTTTGTTAATTAACACAATCGAATTCATGTCGAAATTATTTCGTCGTTTTTCCTCTCATGAATCTCAGGCGATAAACTCGTTGGAGAAGCCAGCAGGATTGTGGTTGCTGAAGCTTGCATACAGGCTCTAGACATCGACTTCACTGAAGGACAGGCTTACGAAATTAACTCAATTGAGGTGAGCTTCCAAGATATGATGCATTTCATCATGAAGCTTGTTTAACAACTGTTTCTAAAAAAAATTTCTATTTTCAAAGATGCTCAAGTCAAAACTCGTTTGAAATAAACAAACTCACTCTTTTTTAGAAAATCATTTCTCTTGCTTCCCTTTATTGTACTTTTATAGAGAATTTTTAGATCAAAGTTTGTGAATAGGCTTTACTGCTACGTTTGGATTTATTAGAAAAAAAATGTTTTTCAAAATGGTCATTTTTATTTAAACTATTTTGATAAAAACGTTTTTAAATGAACTTTGAAAGTGTTTTGAAAGTTATTTTAAGCAACCTAAAATTTCAATTTTTTTTAAAAATCAAATACTTGAAATATTAATCTGAACAAACCCTATAAGTCATTCGAGTTTTTGAAAACGGAAAATAGAAAGCTATTTAAAAACATCATTTCAAACTAGTTGATTCTTGGTAGAGCTCAATCTTTGTCACTTCAGGAAGTAGTAAAATTTTGGAACTTTTTGTAGGGGGAAGGTCCAGGAAATGATCCCCAAAAATGGAGTGAACTATTCAAAGCTGCTGATACACAGTGA

mRNA sequence

ATGGCTACCATTACCAATCTCTCTCTCTCATACATCCCTCATTTTCCTCATCCACCAACAAATTTTCACAAAACATTCCAAACTCTTCCTCTCTTCGCCCTTCCGCCGCTGTGTTCATCTTCATCGTTCGAGAATCAACTTCGCAAGAGCATTTCAAGCTGCAGATTACGATCCCCAGCTATTCGTGCAGTCAATCAAGAAGTTGTTCAGACTCCAAGTTCAGAATCGACCCGTGACTCGAAAACTGGTCCTGTTGCTTCTTCTAAACTCGTCCTTGTTGTCGGAGGCTCCGGCGGCGTTGGACAGTTGGTGGCAGCAGCTTTGCTTGACCGGAATGTCAAATTACGCCTCTTACTGCGTGATCCGGAGAAAGCAAATACATTATTTGGTGAACAAGATGAAAATTTGTTACAGGTATGCAAAGGCGACACAAGGAACCCAGAAGACCTAGATCCATCTATATTTGAGGGTGTCACGCATGTGATTTGCTGCACAGGAACGACAGCCTTTCCTTCAAGGCGATGGGATGGGGACAACACTCCTGAAAGAGTAGATTGGGAGGGTGTGAGAAACCTCATATCAGCAATTCCACGATCAGTAAAGAGAGTAGTTCTTGTTTCATCGATAGGGGTAACCAAATTCAATGAATTGCCGTGGAGCATTATGAATCTTTTTGGCGTTCTCAAATACAAAAAGCAAGGGGAGGATTTTCTTCGAGACTCAGGACTTCCGTTCACAATTATCAGAGCTGGTAGGTTGACTGATGGACCATACACCTCGTATGATCTTAATACTTTGCTCAAAGCTACAGCAGGGCAGCGGCGTGCCGTTCTCCTTGGTCAAGGCGATAAACTCGTTGGAGAAGCCAGCAGGATTGTGGTTGCTGAAGCTTGCATACAGGCTCTAGACATCGACTTCACTGAAGGACAGGCTTACGAAATTAACTCAATTGAGGGGGAAGGTCCAGGAAATGATCCCCAAAAATGGAGTGAACTATTCAAAGCTGCTGATACACAGTGA

Coding sequence (CDS)

ATGGCTACCATTACCAATCTCTCTCTCTCATACATCCCTCATTTTCCTCATCCACCAACAAATTTTCACAAAACATTCCAAACTCTTCCTCTCTTCGCCCTTCCGCCGCTGTGTTCATCTTCATCGTTCGAGAATCAACTTCGCAAGAGCATTTCAAGCTGCAGATTACGATCCCCAGCTATTCGTGCAGTCAATCAAGAAGTTGTTCAGACTCCAAGTTCAGAATCGACCCGTGACTCGAAAACTGGTCCTGTTGCTTCTTCTAAACTCGTCCTTGTTGTCGGAGGCTCCGGCGGCGTTGGACAGTTGGTGGCAGCAGCTTTGCTTGACCGGAATGTCAAATTACGCCTCTTACTGCGTGATCCGGAGAAAGCAAATACATTATTTGGTGAACAAGATGAAAATTTGTTACAGGTATGCAAAGGCGACACAAGGAACCCAGAAGACCTAGATCCATCTATATTTGAGGGTGTCACGCATGTGATTTGCTGCACAGGAACGACAGCCTTTCCTTCAAGGCGATGGGATGGGGACAACACTCCTGAAAGAGTAGATTGGGAGGGTGTGAGAAACCTCATATCAGCAATTCCACGATCAGTAAAGAGAGTAGTTCTTGTTTCATCGATAGGGGTAACCAAATTCAATGAATTGCCGTGGAGCATTATGAATCTTTTTGGCGTTCTCAAATACAAAAAGCAAGGGGAGGATTTTCTTCGAGACTCAGGACTTCCGTTCACAATTATCAGAGCTGGTAGGTTGACTGATGGACCATACACCTCGTATGATCTTAATACTTTGCTCAAAGCTACAGCAGGGCAGCGGCGTGCCGTTCTCCTTGGTCAAGGCGATAAACTCGTTGGAGAAGCCAGCAGGATTGTGGTTGCTGAAGCTTGCATACAGGCTCTAGACATCGACTTCACTGAAGGACAGGCTTACGAAATTAACTCAATTGAGGGGGAAGGTCCAGGAAATGATCCCCAAAAATGGAGTGAACTATTCAAAGCTGCTGATACACAGTGA

Protein sequence

MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPAIRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRDSGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADTQ
Homology
BLAST of PI0005421 vs. ExPASy Swiss-Prot
Match: O80934 (Uncharacterized protein At2g37660, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g37660 PE=1 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 7.4e-24
Identity = 104/358 (29.05%), Postives = 164/358 (45.81%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MA +T  + ++    P  P N +K+      F   P  SS  F + +  S S C      
Sbjct: 1   MAMMTTTTTTFF--HPLLPANTYKSGAVASSFVSVPRSSSLQFRSLVSDSTSIC--GPSK 60

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKL--RLL 120
               N+ V  T S+ +T +  T        VLV G  G  GQ+V   L +R+ +   R L
Sbjct: 61  FTGKNRRVSVTVSAAATTEPLT--------VLVTGAGGRTGQIVYKKLKERSEQFVARGL 120

Query: 121 LRDPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTT---------- 180
           +R  E    + GE      +V  GD R+   + P++ EG+  ++  T             
Sbjct: 121 VRTKESKEKINGED-----EVFIGDIRDTASIAPAV-EGIDALVILTSAVPQMKPGFDPS 180

Query: 181 --AFPSRRWDGDNTPERVDWEGVRNLISAIPRS-VKRVVLVSSIGVTKFNELPWSIMNLF 240
               P   +D    PE+VDW G +N I A   + VK++VLV S+G T  N  P + +   
Sbjct: 181 KGGRPEFFFDDGAYPEQVDWIGQKNQIDAAKAAGVKQIVLVGSMGGTNINH-PLNSIGNA 240

Query: 241 GVLKYKKQGEDFLRDSGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
            +L +K++ E +L DSG+P+TIIRAG L D                G  R +L+G+ D+L
Sbjct: 241 NILVWKRKAEQYLADSGIPYTIIRAGGLQD--------------KDGGIRELLVGKDDEL 300

Query: 301 VGEASRIV----VAEACIQALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADTQ 340
           +   +R +    VAE C+QAL ++  + +A ++ S + EG G   + +  LF    T+
Sbjct: 301 LETETRTIARADVAEVCVQALQLEEAKFKALDLAS-KPEGTGTPTKDFKALFTQVTTK 324

BLAST of PI0005421 vs. ExPASy Swiss-Prot
Match: Q94EG6 (Uncharacterized protein At5g02240 OS=Arabidopsis thaliana OX=3702 GN=At5g02240 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.6e-18
Identity = 82/270 (30.37%), Postives = 132/270 (48.89%), Query Frame = 0

Query: 85  VASSKLVLVVGGSGGVGQLVAAALLDRNVKL--RLLLRDPEKANTLFGEQDENLLQVCKG 144
           +A+   VLV G SG  GQ+V   L + + K   + L+R  +    + GE D     V  G
Sbjct: 1   MANLPTVLVTGASGRTGQIVYKKLKEGSDKFVAKGLVRSAQGKEKIGGEAD-----VFIG 60

Query: 145 DTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT--------------PERVDWEG 204
           D  + + ++P+ F+G+  ++    T+A P  +   D T              PE+VDW G
Sbjct: 61  DITDADSINPA-FQGIDALVIL--TSAVPKMKPGFDPTKGGRPEFIFEDGQYPEQVDWIG 120

Query: 205 VRNLISAIP-RSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRDSGLPFTI 264
            +N I A     VK +V+V S+G T   + P + +    +L +K++ E +L DSG P+TI
Sbjct: 121 QKNQIDAAKVAGVKHIVVVGSMGGTN-PDHPLNKLGNGNILVWKRKAEQYLADSGTPYTI 180

Query: 265 IRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIV----VAEACIQALD 324
           IRAG L D                G  R +L+G+ D+L+   ++ V    VAE CIQAL 
Sbjct: 181 IRAGGLLD--------------KEGGVRELLVGKDDELLQTDTKTVPRADVAEVCIQALL 240

Query: 325 IDFTEGQAYEINSIEGEGPGNDPQKWSELF 334
            +  + +A+++ S + EG     + +  LF
Sbjct: 241 FEEAKNKAFDLGS-KPEGTSTPTKDFKALF 246

BLAST of PI0005421 vs. ExPASy Swiss-Prot
Match: D5JWB3 (Sanguinarine reductase OS=Eschscholzia californica OX=3467 GN=SARED1 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.3e-17
Identity = 90/293 (30.72%), Postives = 137/293 (46.76%), Query Frame = 0

Query: 86  ASSKL-VLVVGGSGGVGQLVAAALLDRNVK--LRLLLRDPEKANTLFGEQDENLLQVCKG 145
           +S KL VL+ G SG  G L    L +R+ K  +R L+R  E +    G  DE  +    G
Sbjct: 4   SSKKLTVLLSGASGLTGSLAFKKLKERSDKFEVRGLVRS-EASKQKLGGGDEIFI----G 63

Query: 146 DTRNPEDLDPSIFEGVTHVICCTG-----------------------------TTAFPSR 205
           D  +P+ L+P++ EG+  +I  T                              +   P  
Sbjct: 64  DISDPKTLEPAM-EGIDALIILTSAIPRMKPTEEFTAEMISGGRSEDVIDASFSGPMPEF 123

Query: 206 RWDGDNTPERVDWEGVRNLI-SAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFG---VLK 265
            +D    PE+VDW G +N I +A    VK +VLV S+G       P   +N  G   +L 
Sbjct: 124 YYDEGQYPEQVDWIGQKNQIDTAKKMGVKHIVLVGSMGGCD----PDHFLNHMGNGNILI 183

Query: 266 YKKQGEDFLRDSGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLV--- 325
           +K++ E +L DSG+P+TIIRAG L +               AG  R +L+ + D L+   
Sbjct: 184 WKRKAEQYLADSGVPYTIIRAGGLDN--------------KAGGVRELLVAKDDVLLPTE 243

Query: 326 -GEASRIVVAEACIQALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADT 339
            G  +R  VAEAC+QAL+I+  + +A+++ S + EG G   + +  LF    T
Sbjct: 244 NGFIARADVAEACVQALEIEEVKNKAFDLGS-KPEGVGEATKDFKALFSQVTT 271

BLAST of PI0005421 vs. ExPASy Swiss-Prot
Match: Q8H124 (Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g34460 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.1e-14
Identity = 72/223 (32.29%), Postives = 106/223 (47.53%), Query Frame = 0

Query: 88  SKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEKANTLFGEQDENLLQVCKGD-TRN 147
           +K V V G +G  G+ +   LL R   ++  +RD EKA T F  +D+  LQ+ + D T  
Sbjct: 46  TKKVFVAGATGQTGKRIVEQLLSRGFAVKAGVRDVEKAKTSF--KDDPSLQIVRADVTEG 105

Query: 148 PEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERVDWEGVRNLISAI-PRSVKRVVL 207
           P+ L   I +    VIC TG      R      TP +VD  G  NL+ A   + V++ VL
Sbjct: 106 PDKLAEVIGDDSQAVICATG-----FRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFVL 165

Query: 208 VSSIGVT-----KFNELPWSIMNLFGV-LKYKKQGEDFLRDSGLPFTIIRAGRLTDGPYT 267
           VSSI V      +     +  +NLFG+ L  K Q E +++ SG+ +TI+R G L + P T
Sbjct: 166 VSSILVNGAAMGQILNPAYLFLNLFGLTLVAKLQAEKYIKKSGINYTIVRPGGLKNDPPT 225

Query: 268 SYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQAL 303
                            V+  +     G  SR +VAE  ++AL
Sbjct: 226 G--------------NVVMEPEDTLYEGSISRDLVAEVAVEAL 247

BLAST of PI0005421 vs. ExPASy Swiss-Prot
Match: Q8H0U5 (Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.5e-13
Identity = 76/252 (30.16%), Postives = 118/252 (46.83%), Query Frame = 0

Query: 69  VQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEKA--- 128
           V T +S +  +SK        LV V G +G VG      LL    ++R  +R  ++A   
Sbjct: 67  VVTEASPTNLNSK-----EEDLVFVAGATGKVGSRTVRELLKLGFRVRAGVRSAQRAGSL 126

Query: 129 ----------NTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRW 188
                     NT  G Q    L++ + D    + + P++    + +ICC G     S + 
Sbjct: 127 VQSVKEMKLQNTDEGTQPVEKLEIVECDLEKKDSIQPAL-GNASVIICCIGA----SEKE 186

Query: 189 DGDNT-PERVDWEGVRNLI-SAIPRSVKRVVLVSSIGVTKFNELPWSIMNLF-GVLKYKK 248
             D T P R+D+   +NL+ +A    V   +LV+S+G  KF   P +I+NLF GVL +K+
Sbjct: 187 ISDITGPYRIDYLATKNLVDAATSAKVNNFILVTSLGTNKFG-FPAAILNLFWGVLCWKR 246

Query: 249 QGEDFLRDSGLPFTIIRAG---RLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEA 300
           + E+ L +SGL + I+R G   R TD    +++L   L  T       L G      G+ 
Sbjct: 247 KAEEALIESGLNYAIVRPGGMERPTDAYKETHNLTLALDDT-------LFG------GQV 294

BLAST of PI0005421 vs. ExPASy TrEMBL
Match: A0A1S3BPR9 (uncharacterized protein At5g02240 OS=Cucumis melo OX=3656 GN=LOC103492002 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.8e-182
Identity = 325/339 (95.87%), Postives = 330/339 (97.35%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MATITNLSLSYIP FPHPPT FH TFQTLPLF LPPLCSSSSF+NQLR SISS RLRSPA
Sbjct: 1   MATITNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPA 60

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120
           IRA+NQEVVQTPSSESTRDSKT PVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR
Sbjct: 61  IRAINQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120

Query: 121 DPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180
           DPEKA TLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT
Sbjct: 121 DPEKATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180

Query: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240
           PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD
Sbjct: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240

Query: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300
           SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ
Sbjct: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300

Query: 301 ALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADTQ 340
           ALDI+FTEGQAYEINSIEGEGPGNDP+KWSELF+AA TQ
Sbjct: 301 ALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAAGTQ 339

BLAST of PI0005421 vs. ExPASy TrEMBL
Match: A0A5D3DW84 (Sanguinarine reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G001030 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.2e-182
Identity = 324/338 (95.86%), Postives = 329/338 (97.34%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MATITNLSLSYIP FPHPPT FH TFQTLPLF LPPLCSSSSF+NQLR SISS RLRSPA
Sbjct: 98  MATITNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPA 157

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120
           IRA+NQEVVQTPSSESTRDSKT PVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR
Sbjct: 158 IRAINQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 217

Query: 121 DPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180
           DPEKA TLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT
Sbjct: 218 DPEKATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 277

Query: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240
           PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD
Sbjct: 278 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 337

Query: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300
           SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ
Sbjct: 338 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 397

Query: 301 ALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADT 339
           ALDI+FTEGQAYEINSIEGEGPGNDP+KWSELF+AA T
Sbjct: 398 ALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAAGT 435

BLAST of PI0005421 vs. ExPASy TrEMBL
Match: A0A5A7VJ33 (Sanguinarine reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G00410 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.2e-182
Identity = 324/338 (95.86%), Postives = 329/338 (97.34%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MATITNLSLSYIP FPHPPT FH TFQTLPLF LPPLCSSSSF+NQLR SISS RLRSPA
Sbjct: 1   MATITNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPA 60

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120
           IRA+NQEVVQTPSSESTRDSKT PVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR
Sbjct: 61  IRAINQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120

Query: 121 DPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180
           DPEKA TLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT
Sbjct: 121 DPEKATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180

Query: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240
           PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD
Sbjct: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240

Query: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300
           SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ
Sbjct: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300

Query: 301 ALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADT 339
           ALDI+FTEGQAYEINSIEGEGPGNDP+KWSELF+AA T
Sbjct: 301 ALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAAGT 338

BLAST of PI0005421 vs. ExPASy TrEMBL
Match: A0A0A0LZU2 (NAD(P)-bd_dom domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G616860 PE=4 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.2e-181
Identity = 325/340 (95.59%), Postives = 328/340 (96.47%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MATIT LSLSYIPHFPHPPT FHKTFQTLPLF LPPLCSSSSFENQLRK+ISSCRL SPA
Sbjct: 1   MATITKLSLSYIPHFPHPPTTFHKTFQTLPLFTLPPLCSSSSFENQLRKTISSCRLPSPA 60

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120
           IRA+NQEVVQTPSS+STRDSKT PVASSK VLVVGGSGGVGQLVAAALLDRNVKLRLLLR
Sbjct: 61  IRAINQEVVQTPSSKSTRDSKTDPVASSKFVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120

Query: 121 DPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180
           DPEKA TLFGEQDE LLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWD DNT
Sbjct: 121 DPEKATTLFGEQDEKLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDEDNT 180

Query: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240
           PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD
Sbjct: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240

Query: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300
           SGLPFTIIR GRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ
Sbjct: 241 SGLPFTIIRPGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300

Query: 301 ALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFK-AADTQ 340
           ALDI FTEGQAYEINSIEGEGPGNDPQKWSELFK AADTQ
Sbjct: 301 ALDIGFTEGQAYEINSIEGEGPGNDPQKWSELFKGAADTQ 340

BLAST of PI0005421 vs. ExPASy TrEMBL
Match: A0A6J1KUJ0 (uncharacterized protein At2g37660, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497739 PE=4 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.0e-165
Identity = 298/332 (89.76%), Postives = 314/332 (94.58%), Query Frame = 0

Query: 5   TNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPAIRAV 64
           +NLSLS+IP F  P  NFHKTFQ +PLF L PLCS S++  Q+RKSISSCR RS AIRA+
Sbjct: 3   SNLSLSFIPEFHRPLGNFHKTFQRVPLFGLHPLCSFSNY--QIRKSISSCRGRSAAIRAI 62

Query: 65  NQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEK 124
           NQEVVQ+PSSES R+SKT PVASSKLVLVVG SGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 63  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 122

Query: 125 ANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERV 184
           A TLFGEQDE++LQV KGDTRNPEDLDPSIFEGVTHVICCTGTTAFPS+RWDGDNTPERV
Sbjct: 123 ATTLFGEQDEDMLQVRKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 182

Query: 185 DWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRDSGLP 244
           DWEGVRNLISA+PRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYK+QGEDFLRDSGLP
Sbjct: 183 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 242

Query: 245 FTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQALDI 304
           FTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQALDI
Sbjct: 243 FTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQALDI 302

Query: 305 DFTEGQAYEINSIEGEGPGNDPQKWSELFKAA 337
           DFTEGQAYEINS+EGEGPGNDPQKWSELF+AA
Sbjct: 303 DFTEGQAYEINSVEGEGPGNDPQKWSELFRAA 332

BLAST of PI0005421 vs. NCBI nr
Match: XP_008450372.1 (PREDICTED: uncharacterized protein At5g02240 [Cucumis melo])

HSP 1 Score: 648.3 bits (1671), Expect = 3.7e-182
Identity = 325/339 (95.87%), Postives = 330/339 (97.35%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MATITNLSLSYIP FPHPPT FH TFQTLPLF LPPLCSSSSF+NQLR SISS RLRSPA
Sbjct: 1   MATITNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPA 60

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120
           IRA+NQEVVQTPSSESTRDSKT PVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR
Sbjct: 61  IRAINQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120

Query: 121 DPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180
           DPEKA TLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT
Sbjct: 121 DPEKATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180

Query: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240
           PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD
Sbjct: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240

Query: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300
           SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ
Sbjct: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300

Query: 301 ALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADTQ 340
           ALDI+FTEGQAYEINSIEGEGPGNDP+KWSELF+AA TQ
Sbjct: 301 ALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAAGTQ 339

BLAST of PI0005421 vs. NCBI nr
Match: KAA0067230.1 (sanguinarine reductase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 324/338 (95.86%), Postives = 329/338 (97.34%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MATITNLSLSYIP FPHPPT FH TFQTLPLF LPPLCSSSSF+NQLR SISS RLRSPA
Sbjct: 1   MATITNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPA 60

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120
           IRA+NQEVVQTPSSESTRDSKT PVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR
Sbjct: 61  IRAINQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120

Query: 121 DPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180
           DPEKA TLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT
Sbjct: 121 DPEKATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180

Query: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240
           PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD
Sbjct: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240

Query: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300
           SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ
Sbjct: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300

Query: 301 ALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADT 339
           ALDI+FTEGQAYEINSIEGEGPGNDP+KWSELF+AA T
Sbjct: 301 ALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAAGT 338

BLAST of PI0005421 vs. NCBI nr
Match: TYK27748.1 (sanguinarine reductase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 324/338 (95.86%), Postives = 329/338 (97.34%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MATITNLSLSYIP FPHPPT FH TFQTLPLF LPPLCSSSSF+NQLR SISS RLRSPA
Sbjct: 98  MATITNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPA 157

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120
           IRA+NQEVVQTPSSESTRDSKT PVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR
Sbjct: 158 IRAINQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 217

Query: 121 DPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180
           DPEKA TLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT
Sbjct: 218 DPEKATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 277

Query: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240
           PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD
Sbjct: 278 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 337

Query: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300
           SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ
Sbjct: 338 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 397

Query: 301 ALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADT 339
           ALDI+FTEGQAYEINSIEGEGPGNDP+KWSELF+AA T
Sbjct: 398 ALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAAGT 435

BLAST of PI0005421 vs. NCBI nr
Match: XP_004139052.1 (uncharacterized protein At5g02240 [Cucumis sativus] >KGN66519.1 hypothetical protein Csa_007127 [Cucumis sativus])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 325/340 (95.59%), Postives = 328/340 (96.47%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MATIT LSLSYIPHFPHPPT FHKTFQTLPLF LPPLCSSSSFENQLRK+ISSCRL SPA
Sbjct: 1   MATITKLSLSYIPHFPHPPTTFHKTFQTLPLFTLPPLCSSSSFENQLRKTISSCRLPSPA 60

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120
           IRA+NQEVVQTPSS+STRDSKT PVASSK VLVVGGSGGVGQLVAAALLDRNVKLRLLLR
Sbjct: 61  IRAINQEVVQTPSSKSTRDSKTDPVASSKFVLVVGGSGGVGQLVAAALLDRNVKLRLLLR 120

Query: 121 DPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT 180
           DPEKA TLFGEQDE LLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWD DNT
Sbjct: 121 DPEKATTLFGEQDEKLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDEDNT 180

Query: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240
           PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD
Sbjct: 181 PERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRD 240

Query: 241 SGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300
           SGLPFTIIR GRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ
Sbjct: 241 SGLPFTIIRPGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQ 300

Query: 301 ALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFK-AADTQ 340
           ALDI FTEGQAYEINSIEGEGPGNDPQKWSELFK AADTQ
Sbjct: 301 ALDIGFTEGQAYEINSIEGEGPGNDPQKWSELFKGAADTQ 340

BLAST of PI0005421 vs. NCBI nr
Match: XP_038878385.1 (uncharacterized protein At2g37660, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 618.2 bits (1593), Expect = 4.1e-173
Identity = 306/333 (91.89%), Postives = 320/333 (96.10%), Query Frame = 0

Query: 7   LSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPAIRAVNQ 66
           LSLS+IPHFP P ++FHKT Q++PLF L PLCS SSFENQ RKSISSCRL+SPAIRA+NQ
Sbjct: 5   LSLSFIPHFPLPASDFHKTSQSVPLFGLAPLCSYSSFENQFRKSISSCRLQSPAIRAINQ 64

Query: 67  EVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEKAN 126
           EVVQ+PSSE+ RD KT PVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEKA 
Sbjct: 65  EVVQSPSSEAIRDRKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEKAT 124

Query: 127 TLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERVDW 186
           TLFGEQDE+LLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERVDW
Sbjct: 125 TLFGEQDEDLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERVDW 184

Query: 187 EGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRDSGLPFT 246
           EGVRNLISA+PRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLR+SGLPFT
Sbjct: 185 EGVRNLISAVPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRESGLPFT 244

Query: 247 IIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQALDIDF 306
           IIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL+GEASRIVVAEACIQALDIDF
Sbjct: 245 IIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLIGEASRIVVAEACIQALDIDF 304

Query: 307 TEGQAYEINSIEGEGPGNDPQKWSELFKAADTQ 340
           TEGQAYEINS+EGEGPGNDPQKWSELFKAA  Q
Sbjct: 305 TEGQAYEINSVEGEGPGNDPQKWSELFKAAQAQ 337

BLAST of PI0005421 vs. TAIR 10
Match: AT4G31530.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 435.3 bits (1118), Expect = 4.6e-122
Identity = 230/341 (67.45%), Postives = 268/341 (78.59%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTL--PLFALPPLCSSSSFENQLRKSISSCRLRS 60
           MAT TNLS +     P   + F  T   +  PLF  P    SS F  +   S+SS     
Sbjct: 1   MATTTNLSFA-----PPSYSRFAATKSQIRNPLFTSPLPLPSSFFLVRNEASLSS---SI 60

Query: 61  PAIRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLL 120
             ++A  +E V T    S         +SSKLVLVVGG+GGVGQLV A+LL RN++ RLL
Sbjct: 61  TPVQAFTEEAVDTSDLAS---------SSSKLVLVVGGTGGVGQLVVASLLKRNIRSRLL 120

Query: 121 LRDPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGD 180
           LRD +KA  LFG+QDE  LQV KGDTRN EDLDPS+FEGVTHVIC TGTTAFPS+RW+ +
Sbjct: 121 LRDLDKATKLFGKQDEYSLQVVKGDTRNAEDLDPSMFEGVTHVICTTGTTAFPSKRWNEE 180

Query: 181 NTPERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFL 240
           NTPE+VDWEGV+NLISA+P SVKRVVLVSS+GVTK NELPWSIMNLFGVLKYKK GEDFL
Sbjct: 181 NTPEKVDWEGVKNLISALPSSVKRVVLVSSVGVTKSNELPWSIMNLFGVLKYKKMGEDFL 240

Query: 241 RDSGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEAC 300
           RDSGLPFTIIR GRLTDGPYTSYDLNTLLKATAG+RRAV++GQGD LVGE SR+VVAEAC
Sbjct: 241 RDSGLPFTIIRPGRLTDGPYTSYDLNTLLKATAGERRAVVIGQGDNLVGEVSRLVVAEAC 300

Query: 301 IQALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADTQ 340
           IQALDI+FT+G+AYEINS++G+GPG+DPQ+W ELFKAA+++
Sbjct: 301 IQALDIEFTQGKAYEINSVKGDGPGSDPQQWRELFKAAESK 324

BLAST of PI0005421 vs. TAIR 10
Match: AT4G31530.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.7e-119
Identity = 230/355 (64.79%), Postives = 268/355 (75.49%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTL--PLFALPPLCSSSSFENQLRKSISSCRLRS 60
           MAT TNLS +     P   + F  T   +  PLF  P    SS F  +   S+SS     
Sbjct: 1   MATTTNLSFA-----PPSYSRFAATKSQIRNPLFTSPLPLPSSFFLVRNEASLSS---SI 60

Query: 61  PAIRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLL 120
             ++A  +E V T    S         +SSKLVLVVGG+GGVGQLV A+LL RN++ RLL
Sbjct: 61  TPVQAFTEEAVDTSDLAS---------SSSKLVLVVGGTGGVGQLVVASLLKRNIRSRLL 120

Query: 121 LRDPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGD 180
           LRD +KA  LFG+QDE  LQV KGDTRN EDLDPS+FEGVTHVIC TGTTAFPS+RW+ +
Sbjct: 121 LRDLDKATKLFGKQDEYSLQVVKGDTRNAEDLDPSMFEGVTHVICTTGTTAFPSKRWNEE 180

Query: 181 NTPERVDWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFL 240
           NTPE+VDWEGV+NLISA+P SVKRVVLVSS+GVTK NELPWSIMNLFGVLKYKK GEDFL
Sbjct: 181 NTPEKVDWEGVKNLISALPSSVKRVVLVSSVGVTKSNELPWSIMNLFGVLKYKKMGEDFL 240

Query: 241 RDSGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEAC 300
           RDSGLPFTIIR GRLTDGPYTSYDLNTLLKATAG+RRAV++GQGD LVGE SR+VVAEAC
Sbjct: 241 RDSGLPFTIIRPGRLTDGPYTSYDLNTLLKATAGERRAVVIGQGDNLVGEVSRLVVAEAC 300

Query: 301 IQALDIDFTEGQAYEINSIE--------------GEGPGNDPQKWSELFKAADTQ 340
           IQALDI+FT+G+AYEINS++              G+GPG+DPQ+W ELFKAA+++
Sbjct: 301 IQALDIEFTQGKAYEINSVKVPQTVSLKFEQSGNGDGPGSDPQQWRELFKAAESK 338

BLAST of PI0005421 vs. TAIR 10
Match: AT2G37660.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 112.8 bits (281), Expect = 5.2e-25
Identity = 104/358 (29.05%), Postives = 164/358 (45.81%), Query Frame = 0

Query: 1   MATITNLSLSYIPHFPHPPTNFHKTFQTLPLFALPPLCSSSSFENQLRKSISSCRLRSPA 60
           MA +T  + ++    P  P N +K+      F   P  SS  F + +  S S C      
Sbjct: 1   MAMMTTTTTTFF--HPLLPANTYKSGAVASSFVSVPRSSSLQFRSLVSDSTSIC--GPSK 60

Query: 61  IRAVNQEVVQTPSSESTRDSKTGPVASSKLVLVVGGSGGVGQLVAAALLDRNVKL--RLL 120
               N+ V  T S+ +T +  T        VLV G  G  GQ+V   L +R+ +   R L
Sbjct: 61  FTGKNRRVSVTVSAAATTEPLT--------VLVTGAGGRTGQIVYKKLKERSEQFVARGL 120

Query: 121 LRDPEKANTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTT---------- 180
           +R  E    + GE      +V  GD R+   + P++ EG+  ++  T             
Sbjct: 121 VRTKESKEKINGED-----EVFIGDIRDTASIAPAV-EGIDALVILTSAVPQMKPGFDPS 180

Query: 181 --AFPSRRWDGDNTPERVDWEGVRNLISAIPRS-VKRVVLVSSIGVTKFNELPWSIMNLF 240
               P   +D    PE+VDW G +N I A   + VK++VLV S+G T  N  P + +   
Sbjct: 181 KGGRPEFFFDDGAYPEQVDWIGQKNQIDAAKAAGVKQIVLVGSMGGTNINH-PLNSIGNA 240

Query: 241 GVLKYKKQGEDFLRDSGLPFTIIRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
            +L +K++ E +L DSG+P+TIIRAG L D                G  R +L+G+ D+L
Sbjct: 241 NILVWKRKAEQYLADSGIPYTIIRAGGLQD--------------KDGGIRELLVGKDDEL 300

Query: 301 VGEASRIV----VAEACIQALDIDFTEGQAYEINSIEGEGPGNDPQKWSELFKAADTQ 340
           +   +R +    VAE C+QAL ++  + +A ++ S + EG G   + +  LF    T+
Sbjct: 301 LETETRTIARADVAEVCVQALQLEEAKFKALDLAS-KPEGTGTPTKDFKALFTQVTTK 324

BLAST of PI0005421 vs. TAIR 10
Match: AT5G02240.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 2.5e-19
Identity = 82/270 (30.37%), Postives = 132/270 (48.89%), Query Frame = 0

Query: 85  VASSKLVLVVGGSGGVGQLVAAALLDRNVKL--RLLLRDPEKANTLFGEQDENLLQVCKG 144
           +A+   VLV G SG  GQ+V   L + + K   + L+R  +    + GE D     V  G
Sbjct: 1   MANLPTVLVTGASGRTGQIVYKKLKEGSDKFVAKGLVRSAQGKEKIGGEAD-----VFIG 60

Query: 145 DTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNT--------------PERVDWEG 204
           D  + + ++P+ F+G+  ++    T+A P  +   D T              PE+VDW G
Sbjct: 61  DITDADSINPA-FQGIDALVIL--TSAVPKMKPGFDPTKGGRPEFIFEDGQYPEQVDWIG 120

Query: 205 VRNLISAIP-RSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRDSGLPFTI 264
            +N I A     VK +V+V S+G T   + P + +    +L +K++ E +L DSG P+TI
Sbjct: 121 QKNQIDAAKVAGVKHIVVVGSMGGTN-PDHPLNKLGNGNILVWKRKAEQYLADSGTPYTI 180

Query: 265 IRAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIV----VAEACIQALD 324
           IRAG L D                G  R +L+G+ D+L+   ++ V    VAE CIQAL 
Sbjct: 181 IRAGGLLD--------------KEGGVRELLVGKDDELLQTDTKTVPRADVAEVCIQALL 240

Query: 325 IDFTEGQAYEINSIEGEGPGNDPQKWSELF 334
            +  + +A+++ S + EG     + +  LF
Sbjct: 241 FEEAKNKAFDLGS-KPEGTSTPTKDFKALF 246

BLAST of PI0005421 vs. TAIR 10
Match: AT2G34460.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 7.6e-16
Identity = 72/223 (32.29%), Postives = 106/223 (47.53%), Query Frame = 0

Query: 88  SKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEKANTLFGEQDENLLQVCKGD-TRN 147
           +K V V G +G  G+ +   LL R   ++  +RD EKA T F  +D+  LQ+ + D T  
Sbjct: 46  TKKVFVAGATGQTGKRIVEQLLSRGFAVKAGVRDVEKAKTSF--KDDPSLQIVRADVTEG 105

Query: 148 PEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERVDWEGVRNLISAI-PRSVKRVVL 207
           P+ L   I +    VIC TG      R      TP +VD  G  NL+ A   + V++ VL
Sbjct: 106 PDKLAEVIGDDSQAVICATG-----FRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFVL 165

Query: 208 VSSIGVT-----KFNELPWSIMNLFGV-LKYKKQGEDFLRDSGLPFTIIRAGRLTDGPYT 267
           VSSI V      +     +  +NLFG+ L  K Q E +++ SG+ +TI+R G L + P T
Sbjct: 166 VSSILVNGAAMGQILNPAYLFLNLFGLTLVAKLQAEKYIKKSGINYTIVRPGGLKNDPPT 225

Query: 268 SYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQAL 303
                            V+  +     G  SR +VAE  ++AL
Sbjct: 226 G--------------NVVMEPEDTLYEGSISRDLVAEVAVEAL 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O809347.4e-2429.05Uncharacterized protein At2g37660, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q94EG63.6e-1830.37Uncharacterized protein At5g02240 OS=Arabidopsis thaliana OX=3702 GN=At5g02240 P... [more]
D5JWB32.3e-1730.72Sanguinarine reductase OS=Eschscholzia californica OX=3467 GN=SARED1 PE=1 SV=1[more]
Q8H1241.1e-1432.29Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q8H0U51.5e-1330.16Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BPR91.8e-18295.87uncharacterized protein At5g02240 OS=Cucumis melo OX=3656 GN=LOC103492002 PE=4 S... [more]
A0A5D3DW845.2e-18295.86Sanguinarine reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A5A7VJ335.2e-18295.86Sanguinarine reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A0A0LZU21.2e-18195.59NAD(P)-bd_dom domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G6168... [more]
A0A6J1KUJ02.0e-16589.76uncharacterized protein At2g37660, chloroplastic OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
XP_008450372.13.7e-18295.87PREDICTED: uncharacterized protein At5g02240 [Cucumis melo][more]
KAA0067230.11.1e-18195.86sanguinarine reductase isoform X1 [Cucumis melo var. makuwa][more]
TYK27748.11.1e-18195.86sanguinarine reductase isoform X1 [Cucumis melo var. makuwa][more]
XP_004139052.12.4e-18195.59uncharacterized protein At5g02240 [Cucumis sativus] >KGN66519.1 hypothetical pro... [more]
XP_038878385.14.1e-17391.89uncharacterized protein At2g37660, chloroplastic isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G31530.14.6e-12267.45NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G31530.23.7e-11964.79NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G37660.15.2e-2529.05NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02240.12.5e-1930.37NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G34460.17.6e-1632.29NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 86..339
e-value: 3.7E-55
score: 189.1
NoneNo IPR availablePANTHERPTHR10366:SF384NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 76..336
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 76..336
NoneNo IPR availableCDDcd05243SDR_a5coord: 105..314
e-value: 9.83048E-52
score: 168.183
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 95..303
e-value: 2.2E-29
score: 102.7
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 87..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005421.1PI0005421.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor