PI0005403 (gene) Melon (PI 482460) v1

Overview
NamePI0005403
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionmetal transporter Nramp5-like
Locationchr03: 12139020 .. 12144600 (+)
RNA-Seq ExpressionPI0005403
SyntenyPI0005403
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACATTTAAAGAAAAATCACAGTTAAAAAAGCTAGTACTGTAAATATTGAAACCTAACCACATTCAAACCAAATTTCAAATCTATGTAACATTTTAAAACACCTAAAACAAACTAAAAAATTTTAAAAGTAAAACTCAAATAGGAAATATAGACTCAAAATCCAATGATTAAAAAAGGTAACATTTTAAAAAAAGAAAATTATCAAATTGGTCTTGGGTCAATGAGTCTAGATATAATAATTGGGGTTGGCTTTACAACATTTTGACTTTTATTAGAAATCTTCCTCTTAGTTTTACTATATATATCTCACAGAAATTTAACTTATGCTCCATCGAATAAAATTAACTAACTTATCAACTCCTTTTTCAACTTTTTTAACTATTTTCAAATATAACAAATTTAATTAAAATATTTATAAAATACTACAATCTATCTACGATATATGTGATAACAAGATAGACTATTATTTGCGTATATCTGTATCATCATAAGAACATATAATAGTCCATCGTCATTTATCATCGAAGGATTGTGATATTTTTTAATATATTTATAAGTATTTTAGAAGTTTTTACTTTAAAATAATTTTCATTTTTACTTTTAAATTAACGACAATATTTATAAATGTAGTGTAAACAAGGACCATGTTTAATTATTTTGATGTGTGTAGCAAATGAAAAAATAAAAAAATGTGGCCACGTCCGACAGAATTGGCTAACCCGTCATTATAAATACGTAAACTGCTTATTATGTTAAATCGTCAAGTCGGCATCGACACTGAATAAGAAAGAAACGGGAAGTCAGCAGATGGAAGCACAGATGAGTACGGCGGCGACACCCTCTTTTGGTGGTGGCAGCAACCGCATAGCCGCGGTTAACATCACAACTCCAACCAACGCCGTAAAGCCACAGAATCACCAAGAAGAAAAAACAACCGCCCATCAGGTCTTTTAATGATCTCTAAAAAATAAAAATAAAAATAAAAATAAAAATTAAATATTTATTTTATTTAGCAAAATGGTAAAATAGAGTTTTTCTTTTCTTTTCATTTTAGTATGTCAATAGTAAATTGAATTAAAATATTTATAAAAATATAGCAAAATTTAAAATTGATAAGTATTTCAATGTCAATGAGTCATATTTGTGTATTAGTATCGGGTAGAATCTAAAATTTTACTACCTTTTATATATTTTGAGTTAAATTTTATTTTAACATTGGATTATTCAGAAACCTGGATGGAGAAAGTTTCTATCACATGTCGGCCCTGGCTTTCTGGTCTCATTAGCTTACCTTGATCCCGGCAATCGTAAGTTTACTGGTCTTTCCGTAAAGAAATTCAACAATACTTGCATCTTGAGGCGATATTAAAAAAATTGATGAAAAAGATAATTATAAATATCCAACACATAAATATTTTAAAAAATTTTGACTTCTGATAATTTCTAATGGTTAAAAAATTTCAAGAGAATTAGACAATAAAGTAAAATTTATTAGATAAAGCATACATACATAACTAAGCAAGTTTTTCTTTTTATTTATTTATTTATTTATCATATAATATATATTTAATTTTAGTTGTTTGAAGTATTTTATTGCTATTGTATTAGTTTTAAAATATATATTTTTAACAAAGTTGTAACCTGACAGGCCATATCGTGTCTTTTCTTTTTCCTATTAATTTGCAGTGGAAACGGACTTGCAGGCTGGAGCTAATCACGGGTTCGAGGTAAACAAAATCAATAAATATATGGTAGAAAAATATGGAGTTTAAGAGCAATTGTCCTCCTTATGCAGAAACTCATGTCAATTATTATCCTTAAACTAACTAATTCAATCAAGTTCTTGTTGCTAAATAATAATTGTAAATTTAAAATTTTGGAAAATTTTCAATACCACATATAAATTTAAGAGATTTCTGTATTATTATAATTTAATAGTGAAAAAATTGTGACGTTTTTAAACACTCATAACTATATATTCCACTAAAAAATAATAGTGGACCGACCAAATCGGCTGAATTGGAGTTGGTTTGATTTTCATAAACTTTAATTTGAGATTGTAATATATGTATATAAGTGCACTTGCTTTTTATAAGAAAAATTATTAATATTAATATTTTCATTTTTTAAAAATAAAATAAAAATATAATATAATGTTGAATTGATGGGCAGCTTCTATGGGTGGTGCTTATCGGATTAATATTTGCACTCATAATCCAATCCCTTGCTGCAAATCTTGGCGTAAGCACTGGTAAGTATTCTAATAATATATATAAATATAGGATAATGTAGAATAATTTTTGTTTTCTATTTTCCCTAACTTGTTTTAATAAATTTTTATTTTAACCTTCAACATGCAAAATCGAAAATTTGTCAGGATTAAAAAGTTAGCTATTAAAGGGTGTCTTTATTTTTTATTTATAAAGAATTAGTTATATATTCTTGAAAAAAACGAAAGGGTGTAATGAAAACTATCTACACTTAATAGGCGTAATATTTAATATATTAAGATAATAATAATGATTTTTAAAAATAAACACCGTAGTTGCTAATATTTATAATTTAGTTCATGCATCCATCCGTATATTTTCAATTTTGTAATAATTTAATCTTTAAATATAGTGGTGACTTTCTATTACGAGAAGTTCCATCAATTTAAATTAAGCATCAATTTTTATTAAATAACGACTTAATACTTATAATTTATAGAAAATCAAATTTATAGATAAATTTGATTTTGATATTTTGTTAGTTATGCGTAAAATTCTTTATTAAATCTTTAATAAAATTTTCAAAATAGAGAAATTGTAAAGTAAAATTTAAGGTGTATGTTAGTACAAATTTAAGTACTATTAACTAAAATTTAAGTATTAAAGTGTTACTTTTAGATCAAAAGTGTTAAAAGATAATTATCTGAGCACAGGACTAACAGACCACGAAAATAATTAATGATTAGTAATTACTTCATTAATTAACACTAATTACTTTTTTATCTTAATTTGTTTTATTTAAGGAAAACATCTTTCAGAAGTGTGCAAAGCCGAATATCCAATATTTGTGAAGTATTGTCTTTGGTTGCTAGCTGAAGTTGCTGTAATAGCAGCCGATATTCCAGAAGGTATTATGTTTAATTTTATTACACTCTAAATGAATTAATTACGTTAATTTGTTTTTGTTAATTTAATTAATTTTATATTGTGTGTGTTTGTTGCAGTGATAGGAACAGCCTTTGCTCTGAATATTCTATTCCATATTCCTGTTTGGGCTGGAGTTCTTCTCACCGGTTTAAGTACCCTTTTGCTTCTTGGTCTCCAAAGATATGGGGTAAATTCATTGAGTCATTAATTATATTTGGTGGTTTAATTTTTGTGGTGTTAGTTTTTAGGAATTAATTAATTAATTAAAAATACTGTACGTAGGCGAGGAAGCTGGAATTGTTGATCGCAATATTAGTGTTTGTAATGGCGGCGTGTTTCTTCGGAGAAATGAGCTACGTGAAGCCTCCGGCGAGCGGTGTTTTAAAAGGAATGTTTGTTCCAAAGCTCAACGGCGAAGGAGCCACCGCCGACGCCATTGCTCTTCTCGGCGCCCTCGTTATGCCGTACTACTACTACCTCTACCTATTCATTATTTATTTATTATAATTATAATATTATTAATTCTGTATAATTTGTGTGTTGAATTAATGGAACTGCGCAGGCATAACCTGTTTCTTCATTCCGCTCTCGTGCTCTCAAGGAAAGTCCCCAATTCCGTCCGTGGTATCAACGTATGTAATAAACCAATTGCTTTTTTATTATATATATATACATAATATATTTATTATTGAAACATTTTAAAAAATCACAAAATATTAAAATTATTTACCAAATATAAAAAATTCATTAAACTCTGTCCATACTTAATTAATTAATTTATTTATTTATTTTTACTATATTTAAAAAATAGTTTGGTTCCCTTAATTTAATTACCAAAATTAAAAAAGGAAAGTTAAATTTATAAACATGGTTTAATTAAAATAAATAAAATTTATTTGTGTTTGATGGGGCATGCAGGACGCGTGTAGGTACTTCCTCATAGAGAGCGGAATTGCATTGTTCATAGCATTTTTAATAAACGTCGCCGTTGTTTCAGTTTCCGGCACCGTTTGTACGGTCGGAAATGTCGCCCAAACCACCGTCGATCAATGCTCTGACATAACTCTCAACTCCGCCTCCTTCCTCCTCCAGGTTCTTTTTTTTTAATTTTCTTTTAACTAGAAAACGCACCGTTTTAATTTTCATCGTTATAATTTAAAATAAATCTTGAATATATTGATTCAGAATGTATTGGGAAAATCAAGCTCCACTATTTACGCCATAGCGTTATTTGCATCGGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGTCAGTTCATCATGCAGGTATTTAATTAATTTAATTTAATTTAAATTATGCATAAATTTAATATACCTTTTTAATTAATTATATTTAATAACAATAACAATAACAATAATAATAAATGCAAATTTTGTTTAATAATTTTGGTTGAAGGGATTTTTGGACTTGAAAATGAAAACATGGGCAAGAAATCTAATGACAAGGTCCATTGCAATAACGCCAAGTCTCATCGTATCCATCATCGGCGGTCCTCAAGGAGCTGGCCGTCTCATCATTATTGCATCGGTAATTTGTTTTTTTTCTTTTTTTCTTTCTTCAAAATTTACAATTAATTAACCTTATATTTTAGAAGTAGAAGCACAAAAGAAAAAAAATATATATATATATATATATATGATGATTGAAATACGTATTCTCAAAACAAAAATATAAAATCAAAATGAGCTTGCATAGACTTGTAATAACGACATGAAGGTGAGGTTTGATTTTTCTACCCATAAATTTTTTAAAATTTTCAAGAATTAATTCAATGGGTTGGTTAATGCGTAATTTGCATCAGATGATCCTTTCCTTCGAGCTTCCGTTTGCTCTAATTCCACTACTCAAATTCAGTAGCAGCAGCACCAAGATGGGCCCCCACAAGAACTCCATATACGTAAGCCTAATCTTCTCCCTTTTCATCTTCCCTATGGCCCACCACATAGTTGGGTCGCCCCATTAACCCATTTTATACTAATCTGGGCTTTTTCCAGATCATTGTAATCTCATGGATATTGGGTCTGGCAATCATCGGCATCAACATCTATTATCTCAGTACGGCTTTCGTGGACTGGCTAATTCACAATAATTTACACAAAGTTGCAAATGTTTTCATTGGGATCGTTGTGTTTCCCTTAATGGCTGCCTACGTTGGTGCCGTTATTTACCTAATGTTTCGAAAAGATAGGGTTGTGACTTACATTGAACCCGAGAAGGATGACGCTATGGTCCAAGCCCATTTGGAAAGTGGGCTTAATAATTCTTTTGGGCCTCGTGACATCACCCCAGTGCCTTTCAGAGAGGATTTGGCCCATATTCCACTACCAGAGTAGAAGACTTGATACCTAATATAGGCATGTATTCAACTCTGGGCTGATGGGCTTTAGTGCCGTCGTGGCCCACTGTTTTTTTA

mRNA sequence

ATGGATACATTTAAAGAAAAATCACAGTTAAAAAAGCTAAAACCTGGATGGAGAAAGTTTCTATCACATGTCGGCCCTGGCTTTCTGGTCTCATTAGCTTACCTTGATCCCGGCAATCTGGAAACGGACTTGCAGGCTGGAGCTAATCACGGGTTCGAGCTTCTATGGGTGGTGCTTATCGGATTAATATTTGCACTCATAATCCAATCCCTTGCTGCAAATCTTGGCGTAAGCACTGGAAAACATCTTTCAGAAGTGTGCAAAGCCGAATATCCAATATTTGTGAAGTATTGTCTTTGGTTGCTAGCTGAAGTTGCTGTAATAGCAGCCGATATTCCAGAAGTGATAGGAACAGCCTTTGCTCTGAATATTCTATTCCATATTCCTGTTTGGGCTGGAGTTCTTCTCACCGGTTTAAGTACCCTTTTGCTTCTTGGTCTCCAAAGATATGGGGCGAGGAAGCTGGAATTGTTGATCGCAATATTAGTGTTTGTAATGGCGGCGTGTTTCTTCGGAGAAATGAGCTACGTGAAGCCTCCGGCGAGCGGTGTTTTAAAAGGAATGTTTGTTCCAAAGCTCAACGGCGAAGGAGCCACCGCCGACGCCATTGCTCTTCTCGGCGCCCTCGTTATGCCGCATAACCTGTTTCTTCATTCCGCTCTCGTGCTCTCAAGGAAAGTCCCCAATTCCGTCCGTGGTATCAACGACGCGTGTAGGTACTTCCTCATAGAGAGCGGAATTGCATTGTTCATAGCATTTTTAATAAACGTCGCCGTTGTTTCAGTTTCCGGCACCGTTTGTACGGTCGGAAATGTCGCCCAAACCACCGTCGATCAATGCTCTGACATAACTCTCAACTCCGCCTCCTTCCTCCTCCAGAATGTATTGGGAAAATCAAGCTCCACTATTTACGCCATAGCGTTATTTGCATCGGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGTCAGTTCATCATGCAGGGATTTTTGGACTTGAAAATGAAAACATGGGCAAGAAATCTAATGACAAGGTCCATTGCAATAACGCCAAGTCTCATCGTATCCATCATCGGCGGTCCTCAAGGAGCTGGCCGTCTCATCATTATTGCATCGATGATCCTTTCCTTCGAGCTTCCGTTTGCTCTAATTCCACTACTCAAATTCAGTAGCAGCAGCACCAAGATGGGCCCCCACAAGAACTCCATATACATCATTGTAATCTCATGGATATTGGGTCTGGCAATCATCGGCATCAACATCTATTATCTCAGTACGGCTTTCGTGGACTGGCTAATTCACAATAATTTACACAAAGTTGCAAATGTTTTCATTGGGATCGTTGTGTTTCCCTTAATGGCTGCCTACGTTGGTGCCGTTATTTACCTAATGTTTCGAAAAGATAGGGTTGTGACTTACATTGAACCCGAGAAGGATGACGCTATGGTCCAAGCCCATTTGGAAAGTGGGCTTAATAATTCTTTTGGGCCTCGTGACATCACCCCAGTGCCTTTCAGAGAGGATTTGGCCCATATTCCACTACCAGAGTAGAAGACTTGATACCTAATATAGGCATGTATTCAACTCTGGGCTGATGGGCTTTAGTGCCGTCGTGGCCCACTGTTTTTTTA

Coding sequence (CDS)

ATGGATACATTTAAAGAAAAATCACAGTTAAAAAAGCTAAAACCTGGATGGAGAAAGTTTCTATCACATGTCGGCCCTGGCTTTCTGGTCTCATTAGCTTACCTTGATCCCGGCAATCTGGAAACGGACTTGCAGGCTGGAGCTAATCACGGGTTCGAGCTTCTATGGGTGGTGCTTATCGGATTAATATTTGCACTCATAATCCAATCCCTTGCTGCAAATCTTGGCGTAAGCACTGGAAAACATCTTTCAGAAGTGTGCAAAGCCGAATATCCAATATTTGTGAAGTATTGTCTTTGGTTGCTAGCTGAAGTTGCTGTAATAGCAGCCGATATTCCAGAAGTGATAGGAACAGCCTTTGCTCTGAATATTCTATTCCATATTCCTGTTTGGGCTGGAGTTCTTCTCACCGGTTTAAGTACCCTTTTGCTTCTTGGTCTCCAAAGATATGGGGCGAGGAAGCTGGAATTGTTGATCGCAATATTAGTGTTTGTAATGGCGGCGTGTTTCTTCGGAGAAATGAGCTACGTGAAGCCTCCGGCGAGCGGTGTTTTAAAAGGAATGTTTGTTCCAAAGCTCAACGGCGAAGGAGCCACCGCCGACGCCATTGCTCTTCTCGGCGCCCTCGTTATGCCGCATAACCTGTTTCTTCATTCCGCTCTCGTGCTCTCAAGGAAAGTCCCCAATTCCGTCCGTGGTATCAACGACGCGTGTAGGTACTTCCTCATAGAGAGCGGAATTGCATTGTTCATAGCATTTTTAATAAACGTCGCCGTTGTTTCAGTTTCCGGCACCGTTTGTACGGTCGGAAATGTCGCCCAAACCACCGTCGATCAATGCTCTGACATAACTCTCAACTCCGCCTCCTTCCTCCTCCAGAATGTATTGGGAAAATCAAGCTCCACTATTTACGCCATAGCGTTATTTGCATCGGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGTCAGTTCATCATGCAGGGATTTTTGGACTTGAAAATGAAAACATGGGCAAGAAATCTAATGACAAGGTCCATTGCAATAACGCCAAGTCTCATCGTATCCATCATCGGCGGTCCTCAAGGAGCTGGCCGTCTCATCATTATTGCATCGATGATCCTTTCCTTCGAGCTTCCGTTTGCTCTAATTCCACTACTCAAATTCAGTAGCAGCAGCACCAAGATGGGCCCCCACAAGAACTCCATATACATCATTGTAATCTCATGGATATTGGGTCTGGCAATCATCGGCATCAACATCTATTATCTCAGTACGGCTTTCGTGGACTGGCTAATTCACAATAATTTACACAAAGTTGCAAATGTTTTCATTGGGATCGTTGTGTTTCCCTTAATGGCTGCCTACGTTGGTGCCGTTATTTACCTAATGTTTCGAAAAGATAGGGTTGTGACTTACATTGAACCCGAGAAGGATGACGCTATGGTCCAAGCCCATTTGGAAAGTGGGCTTAATAATTCTTTTGGGCCTCGTGACATCACCCCAGTGCCTTTCAGAGAGGATTTGGCCCATATTCCACTACCAGAGTAG

Protein sequence

MDTFKEKSQLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQAHLESGLNNSFGPRDITPVPFREDLAHIPLPE
Homology
BLAST of PI0005403 vs. ExPASy Swiss-Prot
Match: Q8H4H5 (Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 4.1e-207
Identity = 370/507 (72.98%), Postives = 431/507 (85.01%), Query Frame = 0

Query: 9   QLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALII 68
           QL   +P W++FL+HVGPGF+VSLAYLDPGNLETDLQAGANH +ELLWV+LIGLIFALII
Sbjct: 33  QLLMKEPAWKRFLAHVGPGFMVSLAYLDPGNLETDLQAGANHRYELLWVILIGLIFALII 92

Query: 69  QSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHI 128
           QSLAANLGV TG+HL+E+CK+EYP FVK  LWLLAE+AVIAADIPEVIGTAFA NILFHI
Sbjct: 93  QSLAANLGVVTGRHLAEICKSEYPKFVKIFLWLLAELAVIAADIPEVIGTAFAFNILFHI 152

Query: 129 PVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGM 188
           PVW GVL+TG STLLLLGLQ+YG RKLE LI++LVFVMAACFFGE+S VKPPA  V+KG+
Sbjct: 153 PVWVGVLITGTSTLLLLGLQKYGVRKLEFLISMLVFVMAACFFGELSIVKPPAKEVMKGL 212

Query: 189 FVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIA 248
           F+P+LNG+GATADAIALLGALVMPHNLFLHSALVLSRK P SVRGI D CR+FL ESG A
Sbjct: 213 FIPRLNGDGATADAIALLGALVMPHNLFLHSALVLSRKTPASVRGIKDGCRFFLYESGFA 272

Query: 249 LFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIAL 308
           LF+A LIN+AVVSVSGT C+  N++Q   D+C++++L+++SFLL+NVLGKSS+ +Y +AL
Sbjct: 273 LFVALLINIAVVSVSGTACSSANLSQEDADKCANLSLDTSSFLLKNVLGKSSAIVYGVAL 332

Query: 309 FASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLI 368
            ASGQSS+ITGTYAGQ+IMQGFLD++M+ W RNLMTR+IAI PSLIVSIIGG +GAGRLI
Sbjct: 333 LASGQSSTITGTYAGQYIMQGFLDIRMRKWLRNLMTRTIAIAPSLIVSIIGGSRGAGRLI 392

Query: 369 IIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAF 428
           IIASMILSFELPFALIPLLKFSSS +KMGPHKNSIYIIV SW LGL IIGIN+Y+LST+F
Sbjct: 393 IIASMILSFELPFALIPLLKFSSSKSKMGPHKNSIYIIVFSWFLGLLIIGINMYFLSTSF 452

Query: 429 VDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQAHLE 488
           V WLIHN+L K ANV +G  VFP M  Y+ AV+YL  RKD VVT++      A+V A  E
Sbjct: 453 VGWLIHNDLPKYANVLVGAAVFPFMLVYIVAVVYLTIRKDSVVTFVADSSLAAVVDA--E 512

Query: 489 SGLNNSFGPRDITPVPFREDLAHIPLP 516
                     D  P+P+R+DLA IPLP
Sbjct: 513 KADAGDLAVDDDEPLPYRDDLADIPLP 537

BLAST of PI0005403 vs. ExPASy Swiss-Prot
Match: Q0D7E4 (Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 2.2e-192
Identity = 340/470 (72.34%), Postives = 403/470 (85.74%), Query Frame = 0

Query: 1   MDTFKEKSQLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLI 60
           +D  +  + L+K +P W++FLSH+GPGF+V LAYLDPGN+ETDLQAGANH +ELLWV+LI
Sbjct: 17  VDDIEALADLRK-EPAWKRFLSHIGPGFMVCLAYLDPGNMETDLQAGANHKYELLWVILI 76

Query: 61  GLIFALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAF 120
           GLIFALIIQSL+ANLGV TG+HL+E+CK EYP++VK CLWLLAE+AVIA+DIPEVIGT F
Sbjct: 77  GLIFALIIQSLSANLGVVTGRHLAELCKTEYPVWVKTCLWLLAELAVIASDIPEVIGTGF 136

Query: 121 ALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPP 180
           A N+LFHIPVW GVL+ G STLLLLGLQRYG RKLE+++A+LVFVMA CFF EMS VKPP
Sbjct: 137 AFNLLFHIPVWTGVLIAGSSTLLLLGLQRYGVRKLEVVVALLVFVMAGCFFVEMSIVKPP 196

Query: 181 ASGVLKGMFVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRY 240
            + VL+G+F+P+L+G GAT D+IALLGALVMPHNLFLHSALVLSR  P S +G+ D CR+
Sbjct: 197 VNEVLQGLFIPRLSGPGATGDSIALLGALVMPHNLFLHSALVLSRNTPASAKGMKDVCRF 256

Query: 241 FLIESGIALFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSS 300
           FL ESGIALF+A L+N+A++SVSGTVC   N++     +CSD+TL+S+SFLL+NVLGKSS
Sbjct: 257 FLFESGIALFVALLVNIAIISVSGTVCNATNLSPEDAVKCSDLTLDSSSFLLRNVLGKSS 316

Query: 301 STIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGG 360
           +T+Y +AL ASGQSS+ITGTYAGQ++MQGFLD+KMK W RNLMTRSIAI PSLIVSIIGG
Sbjct: 317 ATVYGVALLASGQSSTITGTYAGQYVMQGFLDIKMKQWLRNLMTRSIAIVPSLIVSIIGG 376

Query: 361 PQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGIN 420
             GAGRLI+IASMILSFELPFALIPLLKFSSSS KMG +KNSIYI+  SW+LG  IIGIN
Sbjct: 377 SSGAGRLIVIASMILSFELPFALIPLLKFSSSSNKMGENKNSIYIVGFSWVLGFVIIGIN 436

Query: 421 IYYLSTAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRV 471
           IY+LST  V W++HN L   ANV IGIV+FPLM  YV AVIYL FRKD V
Sbjct: 437 IYFLSTKLVGWILHNALPTFANVLIGIVLFPLMLLYVVAVIYLTFRKDTV 485

BLAST of PI0005403 vs. ExPASy Swiss-Prot
Match: Q6ZG85 (Metal transporter NRAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAT1 PE=2 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.6e-174
Identity = 314/506 (62.06%), Postives = 398/506 (78.66%), Query Frame = 0

Query: 6   EKSQLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFA 65
           EK    +++P WRKFL+HVGPG LV++ +LDP NLETD+QAGA+  +ELLWV+L+G++FA
Sbjct: 37  EKDGQFRVQPRWRKFLAHVGPGALVAIGFLDPSNLETDMQAGADFKYELLWVILVGMVFA 96

Query: 66  LIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNIL 125
           L+IQ+LAANLGV TG+HL+E+C+ EYP +V   LW++AE+AVI+ DIPEV+GTAFA NIL
Sbjct: 97  LLIQTLAANLGVKTGRHLAELCREEYPHYVNIFLWIIAELAVISDDIPEVLGTAFAFNIL 156

Query: 126 FHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVL 185
             IPVWAGV+LT  STLLLLG+QR+GARKLE +IA  +F MAACFFGE+SY++P A  V+
Sbjct: 157 LKIPVWAGVILTVFSTLLLLGVQRFGARKLEFIIAAFMFTMAACFFGELSYLRPSAGEVV 216

Query: 186 KGMFVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIES 245
           KGMFVP L G+GA A+AIAL GA++ P+NLFLHSALVLSRK P S + I  ACRYFLIE 
Sbjct: 217 KGMFVPSLQGKGAAANAIALFGAIITPYNLFLHSALVLSRKTPRSDKSIRAACRYFLIEC 276

Query: 246 GIALFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYA 305
            +A  +AFLINV+VV V+G++C   N++    + C D+TL S   LL+NVLG+SSS +YA
Sbjct: 277 SLAFIVAFLINVSVVVVAGSICNANNLSPADANTCGDLTLQSTPLLLRNVLGRSSSVVYA 336

Query: 306 IALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAG 365
           +AL ASGQS++I+ T+AGQ IMQGFLD+KMK W RNL+TR IAI PSLIVSI+ GP GAG
Sbjct: 337 VALLASGQSTTISCTFAGQVIMQGFLDMKMKNWVRNLITRVIAIAPSLIVSIVSGPSGAG 396

Query: 366 RLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLS 425
           +LII++SMILSFELPFALIPLLKF +SS K+GP K SIY +VI+WIL  A+I +N Y+L 
Sbjct: 397 KLIILSSMILSFELPFALIPLLKFCNSSKKVGPLKESIYTVVIAWILSFALIVVNTYFLV 456

Query: 426 TAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIE-PEKDDAMVQ 485
             +VDWL+HNNL K AN  I +VVF LMAAY+ AV+YL FRKD V TY+  PE+  A V+
Sbjct: 457 WTYVDWLVHNNLPKYANGLISVVVFALMAAYLVAVVYLTFRKDTVATYVPVPERAQAQVE 516

Query: 486 AHLESGLNNSFGPRDITPVPFREDLA 511
           A     ++ S    D  P P+R+DLA
Sbjct: 517 AGGTPVVDASAADED-QPAPYRKDLA 541

BLAST of PI0005403 vs. ExPASy Swiss-Prot
Match: Q9SAH8 (Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 4.7e-163
Identity = 294/471 (62.42%), Postives = 373/471 (79.19%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           K  W+ F +++GPGFLVS+AY+DPGN ETDLQAGA++ +ELLW++L+    AL+IQSLAA
Sbjct: 40  KKSWKNFFAYLGPGFLVSIAYIDPGNFETDLQAGAHYKYELLWIILVASCAALVIQSLAA 99

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGV TGKHL+E C+AEY     + LW++AE+AV+A DIPEVIGTAFALN+LF IPVW G
Sbjct: 100 NLGVVTGKHLAEQCRAEYSKVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIG 159

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLSTL+LL LQ+YG RKLE LIA LVF +A CFF E+ Y KP    VL G+FVP+L
Sbjct: 160 VLLTGLSTLILLALQKYGVRKLEFLIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQL 219

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
            G GAT  AI+LLGA+VMPHNLFLHSALVLSRK+P S  GI +ACR++LIESG+AL +AF
Sbjct: 220 KGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSASGIKEACRFYLIESGLALMVAF 279

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LINV+V+SVSG VC   N++      C D+ LN ASFLL+NV+GK SS ++AIAL ASGQ
Sbjct: 280 LINVSVISVSGAVCNAPNLSPEDRANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQ 339

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SS+ITGTYAGQ++MQGFLDL+++ W RNL+TR +AI PSLIV++IGG  GAG+LIIIASM
Sbjct: 340 SSTITGTYAGQYVMQGFLDLRLEPWLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASM 399

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFAL+PLLKF+S  TKMG H N + I  ++W++G  I+GINIYYL ++F+  LI
Sbjct: 400 ILSFELPFALVPLLKFTSCKTKMGSHVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLI 459

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQ 485
           H+++  +  VF GI+ F  +A Y+ A+ YL+FRK+RV T +   +D   V+
Sbjct: 460 HSHMKLILVVFCGILGFAGIALYLAAIAYLVFRKNRVATSLLISRDSQNVE 510

BLAST of PI0005403 vs. ExPASy Swiss-Prot
Match: Q9S9N8 (Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2)

HSP 1 Score: 575.1 bits (1481), Expect = 8.0e-163
Identity = 292/456 (64.04%), Postives = 370/456 (81.14%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           K  W+ F S++GPGFLVS+AY+DPGN ETDLQ+GA + +ELLW++L+    AL+IQSLAA
Sbjct: 32  KKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSGAQYKYELLWIILVASCAALVIQSLAA 91

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGV TGKHL+E C+AEY     + LW++AE+AV+A DIPEVIGTAFALN+LF+IPVW G
Sbjct: 92  NLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFNIPVWIG 151

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLSTL+LL LQ+YG RKLE LIA LVF +A CFF E+ Y KP    VL G+FVP+L
Sbjct: 152 VLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIALCFFVELHYSKPDPKEVLYGLFVPQL 211

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
            G GAT  AI+LLGA+VMPHNLFLHSALVLSRK+P SV GI +ACRY+LIESG+AL +AF
Sbjct: 212 KGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVTGIKEACRYYLIESGLALMVAF 271

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LINV+V+SVSG VC   +++      C D+ LN ASFLL+NV+GK SS ++AIAL ASGQ
Sbjct: 272 LINVSVISVSGAVCNASDLSPEDRASCQDLDLNKASFLLRNVVGKWSSKLFAIALLASGQ 331

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SS+ITGTYAGQ++MQGFLDL+++ W RN +TR +AI PSLIV++IGG  GAG+LIIIASM
Sbjct: 332 SSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCLAIIPSLIVALIGGSAGAGKLIIIASM 391

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFAL+PLLKF+SS TKMG H NS+ I  ++WI+G  I+GINIYYL ++F+  L+
Sbjct: 392 ILSFELPFALVPLLKFTSSKTKMGSHANSLVISSVTWIIGGLIMGINIYYLVSSFIKLLL 451

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDR 470
           H++++ VA VF+G++ F  +A Y+ A+ YL+ RK+R
Sbjct: 452 HSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRKNR 487

BLAST of PI0005403 vs. ExPASy TrEMBL
Match: A0A1S3CA24 (metal transporter Nramp5-like OS=Cucumis melo OX=3656 GN=LOC103498358 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.7e-272
Identity = 494/516 (95.74%), Postives = 501/516 (97.09%), Query Frame = 0

Query: 1   MDTFKEKSQLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLI 60
           +   +E+      KPGWRKF+SHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLI
Sbjct: 34  LQNHQEEKNTTHQKPGWRKFVSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLI 93

Query: 61  GLIFALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAF 120
           GLIFALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAF
Sbjct: 94  GLIFALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAF 153

Query: 121 ALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPP 180
           ALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPP
Sbjct: 154 ALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPP 213

Query: 181 ASGVLKGMFVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRY 240
           AS VLKGMFVPKL GEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRY
Sbjct: 214 ASSVLKGMFVPKLKGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRY 273

Query: 241 FLIESGIALFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSS 300
           FLIESGIALFIAFLINVAVVSVSGTVCTVGNVAQTT DQCSDITLNSASFLLQNVLGKSS
Sbjct: 274 FLIESGIALFIAFLINVAVVSVSGTVCTVGNVAQTTADQCSDITLNSASFLLQNVLGKSS 333

Query: 301 STIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGG 360
           STIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGG
Sbjct: 334 STIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGG 393

Query: 361 PQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGIN 420
           PQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGIN
Sbjct: 394 PQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGIN 453

Query: 421 IYYLSTAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDD 480
           IYYLSTAFVDWLIHNNLHKVANVFIGI VFPLMAAY+GAVIYLMFRKDRVVTYIEPEKDD
Sbjct: 454 IYYLSTAFVDWLIHNNLHKVANVFIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDD 513

Query: 481 AMVQAHLESGLNNSFGPRDITPVPFREDLAHIPLPE 517
           AM QAHLE+GLNNSFGPRD+TPVPFREDLAHI LPE
Sbjct: 514 AMAQAHLETGLNNSFGPRDVTPVPFREDLAHIQLPE 549

BLAST of PI0005403 vs. ExPASy TrEMBL
Match: A0A0A0KDB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G382880 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.6e-270
Identity = 491/503 (97.61%), Postives = 496/503 (98.61%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           KPGWRKFLS+VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA
Sbjct: 47  KPGWRKFLSYVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 106

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGVSTGKHLSEVCKAEYP FVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG
Sbjct: 107 NLGVSTGKHLSEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 166

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL
Sbjct: 167 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 226

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
            GEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF
Sbjct: 227 KGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 286

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LINVAVVSVSGTVCTVGNV QTT DQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ
Sbjct: 287 LINVAVVSVSGTVCTVGNVTQTTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 346

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM
Sbjct: 347 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 406

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI
Sbjct: 407 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 466

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQAHLESGLNN 493
           HNNL KVANVFIGI VFPLMAAY+GAVIYLMFRKDRVVTYIEPEKDDA+ QAHLESGLNN
Sbjct: 467 HNNLPKVANVFIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAVAQAHLESGLNN 526

Query: 494 SFGPRDITPVPFREDLAHIPLPE 517
           SFGPRD+TP+PFREDLAHIPLPE
Sbjct: 527 SFGPRDVTPLPFREDLAHIPLPE 549

BLAST of PI0005403 vs. ExPASy TrEMBL
Match: A0A1S4E5W2 (metal transporter Nramp5-like OS=Cucumis melo OX=3656 GN=LOC103504113 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.5e-268
Identity = 486/503 (96.62%), Postives = 495/503 (98.41%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           KPGWRKF+++VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSL+A
Sbjct: 6   KPGWRKFVAYVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLSA 65

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGVSTGKHLSEVCK+EYPIFVKYCLWLLAEVAVIAADIPEV+GTAFALNILFHIPVWAG
Sbjct: 66  NLGVSTGKHLSEVCKSEYPIFVKYCLWLLAEVAVIAADIPEVVGTAFALNILFHIPVWAG 125

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLST+LLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL
Sbjct: 126 VLLTGLSTILLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 185

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
            GEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSV GINDACRYFLIESGIALFIAF
Sbjct: 186 KGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVSGINDACRYFLIESGIALFIAF 245

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LINVAVVSVSGTVCTVGNVAQTT DQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ
Sbjct: 246 LINVAVVSVSGTVCTVGNVAQTTTDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 305

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM
Sbjct: 306 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 365

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI
Sbjct: 366 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 425

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQAHLESGLNN 493
           HNNL KVANVFIGI VFPLMAAY+GAVIYLMFRKDRVVTYIEPEKDDA+ QAHLE GLNN
Sbjct: 426 HNNLRKVANVFIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAIAQAHLEIGLNN 485

Query: 494 SFGPRDITPVPFREDLAHIPLPE 517
           SFGP DITPVPFREDLAHIPLPE
Sbjct: 486 SFGPGDITPVPFREDLAHIPLPE 508

BLAST of PI0005403 vs. ExPASy TrEMBL
Match: A0A6J1GNU9 (metal transporter Nramp5-like OS=Cucurbita moschata OX=3662 GN=LOC111455783 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 8.9e-258
Identity = 468/512 (91.41%), Postives = 487/512 (95.12%), Query Frame = 0

Query: 5   KEKSQLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF 64
           +E+      KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF
Sbjct: 48  REEPTTTDQKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF 107

Query: 65  ALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNI 124
           ALIIQSLAANLGVSTGKHLSEVCKAEYP FVKYCLWLLAEVAVIAADIPEVIGTAFALNI
Sbjct: 108 ALIIQSLAANLGVSTGKHLSEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNI 167

Query: 125 LFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGV 184
           LFHIPVWAGVLLTGLSTLLL+GLQRYG RKLELLIAILVFVMAACFFGEMSYVKPPA+GV
Sbjct: 168 LFHIPVWAGVLLTGLSTLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGV 227

Query: 185 LKGMFVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIE 244
           LKGMFVPKLNG+GAT DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIE
Sbjct: 228 LKGMFVPKLNGQGATGDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIE 287

Query: 245 SGIALFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIY 304
           SG ALFI+FLIN+AVVSVSGTVCTVG+V+++T DQCSDITLNSASFLLQNVLGKSSSTIY
Sbjct: 288 SGFALFISFLINMAVVSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIY 347

Query: 305 AIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGA 364
           AIAL ASGQSSSITGTYAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGA
Sbjct: 348 AIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGA 407

Query: 365 GRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYL 424
           GRLIIIASMILSFELPFALIPLLKFSSS+TKMGPHKNSIYIIVISWILGL+IIGINIYYL
Sbjct: 408 GRLIIIASMILSFELPFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYL 467

Query: 425 STAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQ 484
           ST FV WLI NNL KVANVFIGIVVFPLMA Y+ AVIYL FRKDRVVTYIEP+K D M Q
Sbjct: 468 STGFVGWLIDNNLPKVANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQ 527

Query: 485 AHLESGLNNSFGPRDITPVPFREDLAHIPLPE 517
           AHLESGL+NSFGP D+ PVPFR+DLAHIPLPE
Sbjct: 528 AHLESGLHNSFGPGDVAPVPFRDDLAHIPLPE 559

BLAST of PI0005403 vs. ExPASy TrEMBL
Match: A0A6J1JPX4 (metal transporter Nramp5-like OS=Cucurbita maxima OX=3661 GN=LOC111487830 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.6e-257
Identity = 466/503 (92.64%), Postives = 484/503 (96.22%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA
Sbjct: 57  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 116

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGVSTGKHLSEVC+AEYP  VKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG
Sbjct: 117 NLGVSTGKHLSEVCRAEYPKLVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 176

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLSTLLL+GLQRYG RKLELLIAILVFVMAACFFGEMSYVKPPA+GVLKGMFVPKL
Sbjct: 177 VLLTGLSTLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKL 236

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
           NG+GAT DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESG ALFI+F
Sbjct: 237 NGQGATGDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISF 296

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LIN+AVVSVSGTVCTVG+V+++T DQCSDITLNSASFLLQNVLGKSSSTIYAIAL ASGQ
Sbjct: 297 LINMAVVSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQ 356

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SSSITGTYAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIASM
Sbjct: 357 SSSITGTYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASM 416

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFALIPLLKFSSS+TKMGPHKNSIYIIVISWILGL+IIGINIYYLSTAFV WLI
Sbjct: 417 ILSFELPFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTAFVGWLI 476

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQAHLESGLNN 493
            NNL KVANVFIGIVVFPLMA Y+ AVIYL FRKDRVVTYIEP+K D M QAHLESGL+N
Sbjct: 477 DNNLPKVANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHN 536

Query: 494 SFGPRDITPVPFREDLAHIPLPE 517
           SFGP D+ PVPFR+DLAHIPLPE
Sbjct: 537 SFGPGDVAPVPFRDDLAHIPLPE 559

BLAST of PI0005403 vs. NCBI nr
Match: XP_008459158.1 (PREDICTED: metal transporter Nramp5-like [Cucumis melo] >XP_008459159.1 PREDICTED: metal transporter Nramp5-like [Cucumis melo])

HSP 1 Score: 948.0 bits (2449), Expect = 3.5e-272
Identity = 494/516 (95.74%), Postives = 501/516 (97.09%), Query Frame = 0

Query: 1   MDTFKEKSQLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLI 60
           +   +E+      KPGWRKF+SHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLI
Sbjct: 34  LQNHQEEKNTTHQKPGWRKFVSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLI 93

Query: 61  GLIFALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAF 120
           GLIFALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAF
Sbjct: 94  GLIFALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAF 153

Query: 121 ALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPP 180
           ALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPP
Sbjct: 154 ALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPP 213

Query: 181 ASGVLKGMFVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRY 240
           AS VLKGMFVPKL GEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRY
Sbjct: 214 ASSVLKGMFVPKLKGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRY 273

Query: 241 FLIESGIALFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSS 300
           FLIESGIALFIAFLINVAVVSVSGTVCTVGNVAQTT DQCSDITLNSASFLLQNVLGKSS
Sbjct: 274 FLIESGIALFIAFLINVAVVSVSGTVCTVGNVAQTTADQCSDITLNSASFLLQNVLGKSS 333

Query: 301 STIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGG 360
           STIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGG
Sbjct: 334 STIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGG 393

Query: 361 PQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGIN 420
           PQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGIN
Sbjct: 394 PQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGIN 453

Query: 421 IYYLSTAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDD 480
           IYYLSTAFVDWLIHNNLHKVANVFIGI VFPLMAAY+GAVIYLMFRKDRVVTYIEPEKDD
Sbjct: 454 IYYLSTAFVDWLIHNNLHKVANVFIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDD 513

Query: 481 AMVQAHLESGLNNSFGPRDITPVPFREDLAHIPLPE 517
           AM QAHLE+GLNNSFGPRD+TPVPFREDLAHI LPE
Sbjct: 514 AMAQAHLETGLNNSFGPRDVTPVPFREDLAHIQLPE 549

BLAST of PI0005403 vs. NCBI nr
Match: XP_011658448.2 (metal transporter Nramp5 [Cucumis sativus] >KGN47700.1 hypothetical protein Csa_018232 [Cucumis sativus])

HSP 1 Score: 941.4 bits (2432), Expect = 3.2e-270
Identity = 491/503 (97.61%), Postives = 496/503 (98.61%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           KPGWRKFLS+VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA
Sbjct: 47  KPGWRKFLSYVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 106

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGVSTGKHLSEVCKAEYP FVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG
Sbjct: 107 NLGVSTGKHLSEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 166

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL
Sbjct: 167 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 226

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
            GEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF
Sbjct: 227 KGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 286

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LINVAVVSVSGTVCTVGNV QTT DQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ
Sbjct: 287 LINVAVVSVSGTVCTVGNVTQTTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 346

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM
Sbjct: 347 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 406

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI
Sbjct: 407 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 466

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQAHLESGLNN 493
           HNNL KVANVFIGI VFPLMAAY+GAVIYLMFRKDRVVTYIEPEKDDA+ QAHLESGLNN
Sbjct: 467 HNNLPKVANVFIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAVAQAHLESGLNN 526

Query: 494 SFGPRDITPVPFREDLAHIPLPE 517
           SFGPRD+TP+PFREDLAHIPLPE
Sbjct: 527 SFGPRDVTPLPFREDLAHIPLPE 549

BLAST of PI0005403 vs. NCBI nr
Match: XP_038886296.1 (metal transporter Nramp5-like [Benincasa hispida])

HSP 1 Score: 938.7 bits (2425), Expect = 2.1e-269
Identity = 488/512 (95.31%), Postives = 499/512 (97.46%), Query Frame = 0

Query: 5   KEKSQLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF 64
           +E+  +   KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF
Sbjct: 47  REEQTINHQKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF 106

Query: 65  ALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNI 124
           ALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNI
Sbjct: 107 ALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNI 166

Query: 125 LFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGV 184
           LFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPA+GV
Sbjct: 167 LFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPATGV 226

Query: 185 LKGMFVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIE 244
           LKGMFVPKLNG+GATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGIN+ACRYFLIE
Sbjct: 227 LKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINEACRYFLIE 286

Query: 245 SGIALFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIY 304
           SG ALFIAFLINVAVVSVSGTVCTVG V++TT DQCSDITLNSASFLLQNVLGKSSSTIY
Sbjct: 287 SGFALFIAFLINVAVVSVSGTVCTVGKVSETTADQCSDITLNSASFLLQNVLGKSSSTIY 346

Query: 305 AIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGA 364
           AIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGA
Sbjct: 347 AIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGA 406

Query: 365 GRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYL 424
           GRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYL
Sbjct: 407 GRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYL 466

Query: 425 STAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQ 484
           STAFV WLIHNNLH++ANVFIGIVVFPLMAAY+ AVIYLMFRKDRVVTYIEPEKDD M Q
Sbjct: 467 STAFVGWLIHNNLHRIANVFIGIVVFPLMAAYILAVIYLMFRKDRVVTYIEPEKDDPMAQ 526

Query: 485 AHLESGLNNSFGPRDITPVPFREDLAHIPLPE 517
           AHLESGLNNSFGPRD TPVPFREDLAHIPLPE
Sbjct: 527 AHLESGLNNSFGPRDFTPVPFREDLAHIPLPE 558

BLAST of PI0005403 vs. NCBI nr
Match: XP_016903614.1 (PREDICTED: metal transporter Nramp5-like [Cucumis melo])

HSP 1 Score: 934.9 bits (2415), Expect = 3.0e-268
Identity = 486/503 (96.62%), Postives = 495/503 (98.41%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           KPGWRKF+++VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSL+A
Sbjct: 6   KPGWRKFVAYVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLSA 65

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGVSTGKHLSEVCK+EYPIFVKYCLWLLAEVAVIAADIPEV+GTAFALNILFHIPVWAG
Sbjct: 66  NLGVSTGKHLSEVCKSEYPIFVKYCLWLLAEVAVIAADIPEVVGTAFALNILFHIPVWAG 125

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLST+LLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL
Sbjct: 126 VLLTGLSTILLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 185

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
            GEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSV GINDACRYFLIESGIALFIAF
Sbjct: 186 KGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVSGINDACRYFLIESGIALFIAF 245

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LINVAVVSVSGTVCTVGNVAQTT DQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ
Sbjct: 246 LINVAVVSVSGTVCTVGNVAQTTTDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 305

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM
Sbjct: 306 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 365

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI
Sbjct: 366 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 425

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQAHLESGLNN 493
           HNNL KVANVFIGI VFPLMAAY+GAVIYLMFRKDRVVTYIEPEKDDA+ QAHLE GLNN
Sbjct: 426 HNNLRKVANVFIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAIAQAHLEIGLNN 485

Query: 494 SFGPRDITPVPFREDLAHIPLPE 517
           SFGP DITPVPFREDLAHIPLPE
Sbjct: 486 SFGPGDITPVPFREDLAHIPLPE 508

BLAST of PI0005403 vs. NCBI nr
Match: XP_022953164.1 (metal transporter Nramp5-like [Cucurbita moschata])

HSP 1 Score: 899.0 bits (2322), Expect = 1.8e-257
Identity = 468/512 (91.41%), Postives = 487/512 (95.12%), Query Frame = 0

Query: 5   KEKSQLKKLKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF 64
           +E+      KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF
Sbjct: 48  REEPTTTDQKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIF 107

Query: 65  ALIIQSLAANLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNI 124
           ALIIQSLAANLGVSTGKHLSEVCKAEYP FVKYCLWLLAEVAVIAADIPEVIGTAFALNI
Sbjct: 108 ALIIQSLAANLGVSTGKHLSEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNI 167

Query: 125 LFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGV 184
           LFHIPVWAGVLLTGLSTLLL+GLQRYG RKLELLIAILVFVMAACFFGEMSYVKPPA+GV
Sbjct: 168 LFHIPVWAGVLLTGLSTLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGV 227

Query: 185 LKGMFVPKLNGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIE 244
           LKGMFVPKLNG+GAT DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIE
Sbjct: 228 LKGMFVPKLNGQGATGDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIE 287

Query: 245 SGIALFIAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIY 304
           SG ALFI+FLIN+AVVSVSGTVCTVG+V+++T DQCSDITLNSASFLLQNVLGKSSSTIY
Sbjct: 288 SGFALFISFLINMAVVSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIY 347

Query: 305 AIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGA 364
           AIAL ASGQSSSITGTYAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGA
Sbjct: 348 AIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGA 407

Query: 365 GRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYL 424
           GRLIIIASMILSFELPFALIPLLKFSSS+TKMGPHKNSIYIIVISWILGL+IIGINIYYL
Sbjct: 408 GRLIIIASMILSFELPFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYL 467

Query: 425 STAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQ 484
           ST FV WLI NNL KVANVFIGIVVFPLMA Y+ AVIYL FRKDRVVTYIEP+K D M Q
Sbjct: 468 STGFVGWLIDNNLPKVANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQ 527

Query: 485 AHLESGLNNSFGPRDITPVPFREDLAHIPLPE 517
           AHLESGL+NSFGP D+ PVPFR+DLAHIPLPE
Sbjct: 528 AHLESGLHNSFGPGDVAPVPFRDDLAHIPLPE 559

BLAST of PI0005403 vs. TAIR 10
Match: AT1G80830.1 (natural resistance-associated macrophage protein 1 )

HSP 1 Score: 575.9 bits (1483), Expect = 3.3e-164
Identity = 294/471 (62.42%), Postives = 373/471 (79.19%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           K  W+ F +++GPGFLVS+AY+DPGN ETDLQAGA++ +ELLW++L+    AL+IQSLAA
Sbjct: 40  KKSWKNFFAYLGPGFLVSIAYIDPGNFETDLQAGAHYKYELLWIILVASCAALVIQSLAA 99

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGV TGKHL+E C+AEY     + LW++AE+AV+A DIPEVIGTAFALN+LF IPVW G
Sbjct: 100 NLGVVTGKHLAEQCRAEYSKVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIG 159

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLSTL+LL LQ+YG RKLE LIA LVF +A CFF E+ Y KP    VL G+FVP+L
Sbjct: 160 VLLTGLSTLILLALQKYGVRKLEFLIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQL 219

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
            G GAT  AI+LLGA+VMPHNLFLHSALVLSRK+P S  GI +ACR++LIESG+AL +AF
Sbjct: 220 KGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSASGIKEACRFYLIESGLALMVAF 279

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LINV+V+SVSG VC   N++      C D+ LN ASFLL+NV+GK SS ++AIAL ASGQ
Sbjct: 280 LINVSVISVSGAVCNAPNLSPEDRANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQ 339

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SS+ITGTYAGQ++MQGFLDL+++ W RNL+TR +AI PSLIV++IGG  GAG+LIIIASM
Sbjct: 340 SSTITGTYAGQYVMQGFLDLRLEPWLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASM 399

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFAL+PLLKF+S  TKMG H N + I  ++W++G  I+GINIYYL ++F+  LI
Sbjct: 400 ILSFELPFALVPLLKFTSCKTKMGSHVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLI 459

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYIEPEKDDAMVQ 485
           H+++  +  VF GI+ F  +A Y+ A+ YL+FRK+RV T +   +D   V+
Sbjct: 460 HSHMKLILVVFCGILGFAGIALYLAAIAYLVFRKNRVATSLLISRDSQNVE 510

BLAST of PI0005403 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 575.1 bits (1481), Expect = 5.7e-164
Identity = 292/456 (64.04%), Postives = 370/456 (81.14%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           K  W+ F S++GPGFLVS+AY+DPGN ETDLQ+GA + +ELLW++L+    AL+IQSLAA
Sbjct: 32  KKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSGAQYKYELLWIILVASCAALVIQSLAA 91

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAG 133
           NLGV TGKHL+E C+AEY     + LW++AE+AV+A DIPEVIGTAFALN+LF+IPVW G
Sbjct: 92  NLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFNIPVWIG 151

Query: 134 VLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKL 193
           VLLTGLSTL+LL LQ+YG RKLE LIA LVF +A CFF E+ Y KP    VL G+FVP+L
Sbjct: 152 VLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIALCFFVELHYSKPDPKEVLYGLFVPQL 211

Query: 194 NGEGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAF 253
            G GAT  AI+LLGA+VMPHNLFLHSALVLSRK+P SV GI +ACRY+LIESG+AL +AF
Sbjct: 212 KGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVTGIKEACRYYLIESGLALMVAF 271

Query: 254 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQ 313
           LINV+V+SVSG VC   +++      C D+ LN ASFLL+NV+GK SS ++AIAL ASGQ
Sbjct: 272 LINVSVISVSGAVCNASDLSPEDRASCQDLDLNKASFLLRNVVGKWSSKLFAIALLASGQ 331

Query: 314 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 373
           SS+ITGTYAGQ++MQGFLDL+++ W RN +TR +AI PSLIV++IGG  GAG+LIIIASM
Sbjct: 332 SSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCLAIIPSLIVALIGGSAGAGKLIIIASM 391

Query: 374 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLI 433
           ILSFELPFAL+PLLKF+SS TKMG H NS+ I  ++WI+G  I+GINIYYL ++F+  L+
Sbjct: 392 ILSFELPFALVPLLKFTSSKTKMGSHANSLVISSVTWIIGGLIMGINIYYLVSSFIKLLL 451

Query: 434 HNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDR 470
           H++++ VA VF+G++ F  +A Y+ A+ YL+ RK+R
Sbjct: 452 HSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRKNR 487

BLAST of PI0005403 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 310.5 bits (794), Expect = 2.6e-84
Identity = 201/485 (41.44%), Postives = 286/485 (58.97%), Query Frame = 0

Query: 14  KPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 73
           K  W+K     GPGFL+S+A+LDPGNLE+DLQAGA  G+ L+W+++      L+IQ L+A
Sbjct: 46  KFSWKKLWLFTGPGFLMSIAFLDPGNLESDLQAGAIAGYSLIWLLMWATAIGLLIQLLSA 105

Query: 74  NLGVSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFH--IPVW 133
            LGV+TG+HL+E+C+ EYP + +  LW++AE+A+I ADI EVIG+A A+ IL +  +P+W
Sbjct: 106 RLGVATGRHLAELCREEYPTWARMVLWIMAEIALIGADIQEVIGSAIAIKILSNGLVPLW 165

Query: 134 AGVLLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVP 193
           AGV++T L   + L L+ YG RKLE + AIL+  MA  F       KP  + +L G  VP
Sbjct: 166 AGVVITALDCFIFLFLENYGIRKLEAVFAILIATMALAFAWMFGQTKPSGTELLVGALVP 225

Query: 194 KLNGEGATADAIALLGALVMPHNLFLHSALVLSRKV-PNSVRGINDACRYFLIESGIALF 253
           KL+       A+ ++G ++MPHN+FLHSALV SR+V P     + +A +Y+ IES  AL 
Sbjct: 226 KLSSR-TIKQAVGIVGCIIMPHNVFLHSALVQSREVDPKKRFRVKEALKYYSIESTGALA 285

Query: 254 IAFLINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSST---IYAIA 313
           ++F+INV V +V         +A T       I L +A   LQ+  G        I+AI 
Sbjct: 286 VSFIINVFVTTVFAKSFYGTEIADT-------IGLANAGQYLQDKYGGGFFPILYIWAIG 345

Query: 314 LFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSII--GGPQGAG 373
           + A+GQSS+ITGTYAGQFIM GFL+LKMK W R L+TRS AI P++IV+++         
Sbjct: 346 VLAAGQSSTITGTYAGQFIMGGFLNLKMKKWVRALITRSCAIIPTMIVALVFDSSDSMLD 405

Query: 374 RLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLS 433
            L    +++ S ++PFA+IPLL   S+   MG  K    +  ISWI+   +I IN Y + 
Sbjct: 406 ELNEWLNVLQSVQIPFAVIPLLCLVSNEQIMGSFKIQPLVQTISWIVAALVIAINGYLMV 465

Query: 434 TAFVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLMFRKDRVVTYI----------E 481
             F             N+ + + V     AYV  V+YL+    R +TY           E
Sbjct: 466 DFFSG--------AATNLILLVPVIIFAIAYVVFVLYLI---SRGLTYTPWQLVASSHKE 511

BLAST of PI0005403 vs. TAIR 10
Match: AT1G47240.1 (NRAMP metal ion transporter 2 )

HSP 1 Score: 305.4 bits (781), Expect = 8.4e-83
Identity = 188/456 (41.23%), Postives = 275/456 (60.31%), Query Frame = 0

Query: 17  WRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLG 76
           WRK     GPGFL+S+A+LDPGNLE DLQAGA  G+ LLW+++      L+IQ L+A +G
Sbjct: 65  WRKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQMLSARVG 124

Query: 77  VSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFH--IPVWAGV 136
           V+TG+HL+E+C+ EYP + +Y LW +AE+A+I ADI EVIG+A A+ IL    +P+WAGV
Sbjct: 125 VATGRHLAELCRDEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLPLWAGV 184

Query: 137 LLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLN 196
           ++T     L L L+ YG RKLE + A+L+  M   F       KP    ++ G+ +P+L+
Sbjct: 185 VITASDCFLFLFLENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGILLPRLS 244

Query: 197 GEGATADAIALLGALVMPHNLFLHSALVLSRKV-PNSVRGINDACRYFLIESGIALFIAF 256
            +     A+ ++G ++MPHN+FLHSALV SRK+ P     + +A  Y+LIES +ALFI+F
Sbjct: 245 SK-TIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESSVALFISF 304

Query: 257 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSST---IYAIALFA 316
           +IN+ V     TV   G       ++ ++I L +A   LQ   G        I+ I L A
Sbjct: 305 MINLFVT----TVFAKGFYG---TEKANNIGLVNAGQYLQEKFGGGLLPILYIWGIGLLA 364

Query: 317 SGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLII- 376
           +GQSS+ITGTYAGQFIM GFL+L++K W R ++TRS AI P++IV+I+     A   ++ 
Sbjct: 365 AGQSSTITGTYAGQFIMGGFLNLRLKKWMRAVITRSCAIVPTMIVAIVFNTSEASLDVLN 424

Query: 377 -IASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAF 436
              +++ S ++PFAL+PLL   S    MG  K    +  I+W +   ++ IN Y L   F
Sbjct: 425 EWLNVLQSVQIPFALLPLLTLVSKEEIMGDFKIGPILQRIAWTVAALVMIINGYLLLDFF 484

Query: 437 VDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLM 465
           V         +V     G+ V     AY+  ++YL+
Sbjct: 485 VS--------EVDGFLFGVTVCVWTTAYIAFIVYLI 504

BLAST of PI0005403 vs. TAIR 10
Match: AT4G18790.1 (NRAMP metal ion transporter family protein )

HSP 1 Score: 301.6 bits (771), Expect = 1.2e-81
Identity = 186/477 (38.99%), Postives = 281/477 (58.91%), Query Frame = 0

Query: 17  WRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLG 76
           W K     GPGFL+S+A+LDPGN+E DLQAGA  G+ LLW++L   +  L++Q L+A +G
Sbjct: 67  WAKLWKFTGPGFLMSIAFLDPGNIEGDLQAGAVAGYSLLWLLLWATLMGLLMQLLSARIG 126

Query: 77  VSTGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFH--IPVWAGV 136
           V+TG+HL+E+C++EYP + +  LW +AEVA+I ADI EVIG+A AL IL    +P+W GV
Sbjct: 127 VATGRHLAEICRSEYPSWARILLWFMAEVALIGADIQEVIGSAIALQILTRGFLPIWVGV 186

Query: 137 LLTGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLN 196
           ++T     L+  L++ G RKLE L A+L+  MA  F    +  KP    +  G+ +PKL 
Sbjct: 187 IITSFDCFLISYLEKCGMRKLEGLFAVLIATMALSFAWMFNETKPSVEELFIGIIIPKL- 246

Query: 197 GEGATADAIALLGALVMPHNLFLHSALVLSRKV-PNSVRGINDACRYFLIESGIALFIAF 256
           G     +A+ ++G ++ PHN+FLHSALV SRK  P  +  + +A  Y+ IES  ALF++F
Sbjct: 247 GSKTIREAVGVVGCVITPHNVFLHSALVQSRKTDPKEINRVQEALNYYTIESSAALFVSF 306

Query: 257 LINVAVVSVSGTVCTVGNVAQTTVDQCSDITLNSASFLLQNVLGKSSST---IYAIALFA 316
           +IN+ V +V                Q   I L +A + LQ   G        I+ I L A
Sbjct: 307 MINLFVTAVFAK-------GFYGTKQADSIGLVNAGYYLQEKYGGGVFPILYIWGIGLLA 366

Query: 317 SGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIII 376
           +GQSS+ITGTYAGQFIM+GFLDL+M+ W    +TRS AI P++ V+I+      G L ++
Sbjct: 367 AGQSSTITGTYAGQFIMEGFLDLQMEQWLSAFITRSFAIVPTMFVAIMFN-TSEGSLDVL 426

Query: 377 ---ASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTA 436
               +++ S ++PFA+IPLL   S+   MG  K    +  ++W + + ++ IN Y L   
Sbjct: 427 NEWLNILQSMQIPFAVIPLLTMVSNEHIMGVFKIGPSLEKLAWTVAVFVMMINGYLLLDF 486

Query: 437 FVDWLIHNNLHKVANVFIGIVVFPLMAAYVGAVIYLM-FRKDRVVTYIEPEKDDAMV 484
           F        + +V    +G +VF  +  Y+  +IYL+ +R  +  ++   E  + +V
Sbjct: 487 F--------MAEVEGFLVGFLVFGGVVGYISFIIYLVSYRSSQSSSWSSLEMSERVV 526

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H4H54.1e-20772.98Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2... [more]
Q0D7E42.2e-19272.34Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2... [more]
Q6ZG851.6e-17462.06Metal transporter NRAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAT1 PE=2 S... [more]
Q9SAH84.7e-16362.42Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1[more]
Q9S9N88.0e-16364.04Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3CA241.7e-27295.74metal transporter Nramp5-like OS=Cucumis melo OX=3656 GN=LOC103498358 PE=3 SV=1[more]
A0A0A0KDB81.6e-27097.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G382880 PE=3 SV=1[more]
A0A1S4E5W21.5e-26896.62metal transporter Nramp5-like OS=Cucumis melo OX=3656 GN=LOC103504113 PE=3 SV=1[more]
A0A6J1GNU98.9e-25891.41metal transporter Nramp5-like OS=Cucurbita moschata OX=3662 GN=LOC111455783 PE=3... [more]
A0A6J1JPX42.6e-25792.64metal transporter Nramp5-like OS=Cucurbita maxima OX=3661 GN=LOC111487830 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_008459158.13.5e-27295.74PREDICTED: metal transporter Nramp5-like [Cucumis melo] >XP_008459159.1 PREDICTE... [more]
XP_011658448.23.2e-27097.61metal transporter Nramp5 [Cucumis sativus] >KGN47700.1 hypothetical protein Csa_... [more]
XP_038886296.12.1e-26995.31metal transporter Nramp5-like [Benincasa hispida][more]
XP_016903614.13.0e-26896.62PREDICTED: metal transporter Nramp5-like [Cucumis melo][more]
XP_022953164.11.8e-25791.41metal transporter Nramp5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G80830.13.3e-16462.42natural resistance-associated macrophage protein 1 [more]
AT1G15960.15.7e-16464.04NRAMP metal ion transporter 6 [more]
AT5G67330.12.6e-8441.44natural resistance associated macrophage protein 4 [more]
AT1G47240.18.4e-8341.23NRAMP metal ion transporter 2 [more]
AT4G18790.11.2e-8138.99NRAMP metal ion transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001046NRAMP familyPRINTSPR00447NATRESASSCMPcoord: 127..146
score: 44.0
coord: 202..225
score: 63.0
coord: 340..357
score: 53.78
coord: 152..173
score: 41.27
coord: 99..125
score: 57.33
coord: 372..391
score: 49.2
coord: 312..331
score: 73.0
IPR001046NRAMP familyPFAMPF01566Nrampcoord: 41..402
e-value: 8.8E-116
score: 386.8
IPR001046NRAMP familyTIGRFAMTIGR01197TIGR01197coord: 18..394
e-value: 5.0E-120
score: 399.3
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 10..516
IPR001046NRAMP familyHAMAPMF_00221NRAMPcoord: 6..428
score: 37.893818
NoneNo IPR availablePANTHERPTHR11706:SF77METAL TRANSPORTER NRAMP5coord: 10..516

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005403.1PI0005403.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070574 cadmium ion transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:0030001 metal ion transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015086 cadmium ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity