PI0005378 (gene) Melon (PI 482460) v1

Overview
NamePI0005378
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGag/pol protein
Locationchr07: 7613983 .. 7614567 (+)
RNA-Seq ExpressionPI0005378
SyntenyPI0005378
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAATGTCCTCCTTCCCCTTCTCAAAATGCATCCCAGACTGTCAGGGATGCATATGACCGTTGGACAAAGGCCAATGATAAGGCTCGCGTCTACATCTTGGCCAGTATGTCTGACGTACTGATCAAGAAACATGAGACCATGGTCACTGCACGTCAGATCATGGACTCTCTTCAAGAGATGTTTGGACAACCGTCCACTCAGATCTGGCAAGAGGCAATTAAACACGTTTATAATGCCCGTTTGAATGAGGGTCAATCTATTAGAGAACATGTTCTTGACTTGATAGTCCATTTCAACGAAGCGGTCTTTGATGAGAAGAGTCAAGTGTCCTTTATCTTGAAATCTCTTCTAAAGAGCTTTCTTCAATTCTGTAGCAATACGGTTATGAACAAGATAGAGTACAATATGACTTCCCTCCTTAATGAGTTGCAGATTTTTCAGTCTCTTATGAATAATGAGGGACAGAAAGAAGGAGAGGCAAATGTTGCCCATTCCAGGAGGTTTCAGAAGGATTCATCCTCTGGAACCAAGTCTGCACCTTCATTTTCTGGCACTAAGAAAATCCAGAAGAAGAAATGA

mRNA sequence

ATGGAGGAATGTCCTCCTTCCCCTTCTCAAAATGCATCCCAGACTGTCAGGGATGCATATGACCGTTGGACAAAGGCCAATGATAAGGCTCGCGTCTACATCTTGGCCAGTATGTCTGACGTACTGATCAAGAAACATGAGACCATGGTCACTGCACGTCAGATCATGGACTCTCTTCAAGAGATGTTTGGACAACCGTCCACTCAGATCTGGCAAGAGGCAATTAAACACGTTTATAATGCCCGTTTGAATGAGGGTCAATCTATTAGAGAACATGTTCTTGACTTGATAGTCCATTTCAACGAAGCGGTCTTTGATGAGAAGAGTCAAGTGTCCTTTATCTTGAAATCTCTTCTAAAGAGCTTTCTTCAATTCTGTAGCAATACGGTTATGAACAAGATAGAGTACAATATGACTTCCCTCCTTAATGAGTTGCAGATTTTTCAGTCTCTTATGAATAATGAGGGACAGAAAGAAGGAGAGGCAAATGTTGCCCATTCCAGGAGGTTTCAGAAGGATTCATCCTCTGGAACCAAGTCTGCACCTTCATTTTCTGGCACTAAGAAAATCCAGAAGAAGAAATGA

Coding sequence (CDS)

ATGGAGGAATGTCCTCCTTCCCCTTCTCAAAATGCATCCCAGACTGTCAGGGATGCATATGACCGTTGGACAAAGGCCAATGATAAGGCTCGCGTCTACATCTTGGCCAGTATGTCTGACGTACTGATCAAGAAACATGAGACCATGGTCACTGCACGTCAGATCATGGACTCTCTTCAAGAGATGTTTGGACAACCGTCCACTCAGATCTGGCAAGAGGCAATTAAACACGTTTATAATGCCCGTTTGAATGAGGGTCAATCTATTAGAGAACATGTTCTTGACTTGATAGTCCATTTCAACGAAGCGGTCTTTGATGAGAAGAGTCAAGTGTCCTTTATCTTGAAATCTCTTCTAAAGAGCTTTCTTCAATTCTGTAGCAATACGGTTATGAACAAGATAGAGTACAATATGACTTCCCTCCTTAATGAGTTGCAGATTTTTCAGTCTCTTATGAATAATGAGGGACAGAAAGAAGGAGAGGCAAATGTTGCCCATTCCAGGAGGTTTCAGAAGGATTCATCCTCTGGAACCAAGTCTGCACCTTCATTTTCTGGCACTAAGAAAATCCAGAAGAAGAAATGA

Protein sequence

MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQEMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHFNEAVFDEKSQVSFILKSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEANVAHSRRFQKDSSSGTKSAPSFSGTKKIQKKK
Homology
BLAST of PI0005378 vs. ExPASy TrEMBL
Match: A0A5D3D0D9 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold94G00210 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.3e-60
Identity = 127/167 (76.05%), Postives = 145/167 (86.83%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           MEECPP P+QNASQ+V+DAYD WTKANDKA +Y+LAS+SD+L KKHE MVTARQIMDSL+
Sbjct: 40  MEECPPFPTQNASQSVKDAYDHWTKANDKASLYMLASLSDILSKKHEIMVTARQIMDSLR 99

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHF-----NEAVFDEKSQVSFIL 120
           EMFGQPS QI QEAIK+VYNAR+ EGQS+REHVL +IV+F     N+A+FDEKSQVS+IL
Sbjct: 100 EMFGQPSIQIKQEAIKYVYNARMKEGQSVREHVLGMIVNFNVAEMNKAIFDEKSQVSYIL 159

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEA 163
           KSL KSFLQFCSN  MNKIEYNMT+LL ELQ FQSL   +GQKEGEA
Sbjct: 160 KSLSKSFLQFCSNVKMNKIEYNMTTLLKELQTFQSL---KGQKEGEA 203

BLAST of PI0005378 vs. ExPASy TrEMBL
Match: A0A5A7VA67 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold616G00110 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.3e-60
Identity = 127/167 (76.05%), Postives = 145/167 (86.83%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           MEECPP P+QNASQ+V+DAYD WTKANDKA +Y+LAS+SD+L KKHE MVTARQIMDSL+
Sbjct: 40  MEECPPFPTQNASQSVKDAYDHWTKANDKASLYMLASLSDILSKKHEIMVTARQIMDSLR 99

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHF-----NEAVFDEKSQVSFIL 120
           EMFGQPS QI QEAIK+VYNAR+ EGQS+REHVL +IV+F     N+A+FDEKSQVS+IL
Sbjct: 100 EMFGQPSIQIKQEAIKYVYNARMKEGQSVREHVLGMIVNFNVAEMNKAIFDEKSQVSYIL 159

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEA 163
           KSL KSFLQFCSN  MNKIEYNMT+LL ELQ FQSL   +GQKEGEA
Sbjct: 160 KSLSKSFLQFCSNVKMNKIEYNMTTLLKELQTFQSL---KGQKEGEA 203

BLAST of PI0005378 vs. ExPASy TrEMBL
Match: A0A5D3DDU7 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1073G00010 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 5.0e-60
Identity = 128/173 (73.99%), Postives = 144/173 (83.24%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           M+ECPP P+QNASQ+V+D YDRWTKANDKARVYILASMSD+L KKHE MVTA QIMDSL+
Sbjct: 50  MDECPPFPTQNASQSVKDTYDRWTKANDKARVYILASMSDILSKKHEIMVTACQIMDSLR 109

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHFNEA-----VFDEKSQVSFIL 120
           EMFGQ S QI Q+AIK+VYNA + EGQS+R+HV+D+IVHFN A     VFDEKSQ+SFIL
Sbjct: 110 EMFGQSSIQIKQDAIKYVYNAHMKEGQSVRDHVIDMIVHFNVAEMNGVVFDEKSQMSFIL 169

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEANVAHSR 169
           KSL KSFLQF SN  MNKI YNMT+ L EL  FQSL   +GQKEGEANV HSR
Sbjct: 170 KSLPKSFLQFPSNAEMNKINYNMTTFLKELHTFQSL---KGQKEGEANVVHSR 219

BLAST of PI0005378 vs. ExPASy TrEMBL
Match: A0A5A7V6N0 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold749G00160 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 6.5e-60
Identity = 133/200 (66.50%), Postives = 163/200 (81.50%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           +EECP  P+ NA+QTVR+ Y+RW KAN+KAR YILAS+S+VL KKHE+M+TAR+IMDSLQ
Sbjct: 40  VEECPQVPAANATQTVREPYERWAKANEKARAYILASLSEVLAKKHESMLTAREIMDSLQ 99

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHF-----NEAVFDEKSQVSFIL 120
           EMFGQ S QI  +A+K++YNAR+NEG S+REHVL+++VHF     N AV DE SQVSFIL
Sbjct: 100 EMFGQASYQIKHDALKYIYNARMNEGASVREHVLNIMVHFNVAEMNGAVIDEASQVSFIL 159

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEANVAHS-RRFQKDS 180
           +SL +SFLQF SN VMNKI Y +T+LLNELQ F+SLM  +GQK GEANVA S R+F + S
Sbjct: 160 ESLPESFLQFRSNAVMNKIAYTLTTLLNELQTFESLMKIKGQK-GEANVATSTRKFHRGS 219

Query: 181 SSGTKSAPSFSGTKKIQKKK 195
           +SGTKS PS SG KK +KKK
Sbjct: 220 TSGTKSMPSSSGNKKWKKKK 238

BLAST of PI0005378 vs. ExPASy TrEMBL
Match: A0A5A7SNP8 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G002160 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 6.5e-60
Identity = 132/200 (66.00%), Postives = 164/200 (82.00%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           +E+CP  P+ NA++TVR+ Y+RW KAN+KAR YILAS+S+VL KKHE+M+TAR+IMDSLQ
Sbjct: 102 VEDCPQVPAANATRTVREPYERWAKANEKARAYILASLSEVLAKKHESMLTAREIMDSLQ 161

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHF-----NEAVFDEKSQVSFIL 120
           EMFGQ S QI  +A+K++YNAR+NEG S+REHVL+++VHF     NEAV DE SQVSFIL
Sbjct: 162 EMFGQASYQIKHDALKYIYNARMNEGASVREHVLNMMVHFNVAVMNEAVIDEASQVSFIL 221

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEANVAHS-RRFQKDS 180
           +SL +SFLQF SN VMNKI Y +T+LLNELQ F+SLM  +GQK GEANVA S R+F + S
Sbjct: 222 ESLPESFLQFRSNAVMNKIAYTLTTLLNELQTFESLMKIKGQK-GEANVATSTRKFHRGS 281

Query: 181 SSGTKSAPSFSGTKKIQKKK 195
           +SGTKS PS SG KK +KKK
Sbjct: 282 TSGTKSMPSSSGNKKWKKKK 300

BLAST of PI0005378 vs. NCBI nr
Match: KAA0063887.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 242.7 bits (618), Expect = 2.7e-60
Identity = 127/167 (76.05%), Postives = 145/167 (86.83%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           MEECPP P+QNASQ+V+DAYD WTKANDKA +Y+LAS+SD+L KKHE MVTARQIMDSL+
Sbjct: 40  MEECPPFPTQNASQSVKDAYDHWTKANDKASLYMLASLSDILSKKHEIMVTARQIMDSLR 99

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHF-----NEAVFDEKSQVSFIL 120
           EMFGQPS QI QEAIK+VYNAR+ EGQS+REHVL +IV+F     N+A+FDEKSQVS+IL
Sbjct: 100 EMFGQPSIQIKQEAIKYVYNARMKEGQSVREHVLGMIVNFNVAEMNKAIFDEKSQVSYIL 159

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEA 163
           KSL KSFLQFCSN  MNKIEYNMT+LL ELQ FQSL   +GQKEGEA
Sbjct: 160 KSLSKSFLQFCSNVKMNKIEYNMTTLLKELQTFQSL---KGQKEGEA 203

BLAST of PI0005378 vs. NCBI nr
Match: TYK15919.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 242.7 bits (618), Expect = 2.7e-60
Identity = 127/167 (76.05%), Postives = 145/167 (86.83%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           MEECPP P+QNASQ+V+DAYD WTKANDKA +Y+LAS+SD+L KKHE MVTARQIMDSL+
Sbjct: 40  MEECPPFPTQNASQSVKDAYDHWTKANDKASLYMLASLSDILSKKHEIMVTARQIMDSLR 99

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHF-----NEAVFDEKSQVSFIL 120
           EMFGQPS QI QEAIK+VYNAR+ EGQS+REHVL +IV+F     N+A+FDEKSQVS+IL
Sbjct: 100 EMFGQPSIQIKQEAIKYVYNARMKEGQSVREHVLGMIVNFNVAEMNKAIFDEKSQVSYIL 159

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEA 163
           KSL KSFLQFCSN  MNKIEYNMT+LL ELQ FQSL   +GQKEGEA
Sbjct: 160 KSLSKSFLQFCSNVKMNKIEYNMTTLLKELQTFQSL---KGQKEGEA 203

BLAST of PI0005378 vs. NCBI nr
Match: TYK21841.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 240.7 bits (613), Expect = 1.0e-59
Identity = 128/173 (73.99%), Postives = 144/173 (83.24%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           M+ECPP P+QNASQ+V+D YDRWTKANDKARVYILASMSD+L KKHE MVTA QIMDSL+
Sbjct: 50  MDECPPFPTQNASQSVKDTYDRWTKANDKARVYILASMSDILSKKHEIMVTACQIMDSLR 109

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHFNEA-----VFDEKSQVSFIL 120
           EMFGQ S QI Q+AIK+VYNA + EGQS+R+HV+D+IVHFN A     VFDEKSQ+SFIL
Sbjct: 110 EMFGQSSIQIKQDAIKYVYNAHMKEGQSVRDHVIDMIVHFNVAEMNGVVFDEKSQMSFIL 169

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEANVAHSR 169
           KSL KSFLQF SN  MNKI YNMT+ L EL  FQSL   +GQKEGEANV HSR
Sbjct: 170 KSLPKSFLQFPSNAEMNKINYNMTTFLKELHTFQSL---KGQKEGEANVVHSR 219

BLAST of PI0005378 vs. NCBI nr
Match: KAA0061339.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 240.4 bits (612), Expect = 1.3e-59
Identity = 133/200 (66.50%), Postives = 163/200 (81.50%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           +EECP  P+ NA+QTVR+ Y+RW KAN+KAR YILAS+S+VL KKHE+M+TAR+IMDSLQ
Sbjct: 40  VEECPQVPAANATQTVREPYERWAKANEKARAYILASLSEVLAKKHESMLTAREIMDSLQ 99

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHF-----NEAVFDEKSQVSFIL 120
           EMFGQ S QI  +A+K++YNAR+NEG S+REHVL+++VHF     N AV DE SQVSFIL
Sbjct: 100 EMFGQASYQIKHDALKYIYNARMNEGASVREHVLNIMVHFNVAEMNGAVIDEASQVSFIL 159

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEANVAHS-RRFQKDS 180
           +SL +SFLQF SN VMNKI Y +T+LLNELQ F+SLM  +GQK GEANVA S R+F + S
Sbjct: 160 ESLPESFLQFRSNAVMNKIAYTLTTLLNELQTFESLMKIKGQK-GEANVATSTRKFHRGS 219

Query: 181 SSGTKSAPSFSGTKKIQKKK 195
           +SGTKS PS SG KK +KKK
Sbjct: 220 TSGTKSMPSSSGNKKWKKKK 238

BLAST of PI0005378 vs. NCBI nr
Match: KAA0026233.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 240.4 bits (612), Expect = 1.3e-59
Identity = 132/200 (66.00%), Postives = 164/200 (82.00%), Query Frame = 0

Query: 1   MEECPPSPSQNASQTVRDAYDRWTKANDKARVYILASMSDVLIKKHETMVTARQIMDSLQ 60
           +E+CP  P+ NA++TVR+ Y+RW KAN+KAR YILAS+S+VL KKHE+M+TAR+IMDSLQ
Sbjct: 102 VEDCPQVPAANATRTVREPYERWAKANEKARAYILASLSEVLAKKHESMLTAREIMDSLQ 161

Query: 61  EMFGQPSTQIWQEAIKHVYNARLNEGQSIREHVLDLIVHF-----NEAVFDEKSQVSFIL 120
           EMFGQ S QI  +A+K++YNAR+NEG S+REHVL+++VHF     NEAV DE SQVSFIL
Sbjct: 162 EMFGQASYQIKHDALKYIYNARMNEGASVREHVLNMMVHFNVAVMNEAVIDEASQVSFIL 221

Query: 121 KSLLKSFLQFCSNTVMNKIEYNMTSLLNELQIFQSLMNNEGQKEGEANVAHS-RRFQKDS 180
           +SL +SFLQF SN VMNKI Y +T+LLNELQ F+SLM  +GQK GEANVA S R+F + S
Sbjct: 222 ESLPESFLQFRSNAVMNKIAYTLTTLLNELQTFESLMKIKGQK-GEANVATSTRKFHRGS 281

Query: 181 SSGTKSAPSFSGTKKIQKKK 195
           +SGTKS PS SG KK +KKK
Sbjct: 282 TSGTKSMPSSSGNKKWKKKK 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3D0D91.3e-6076.05Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold94G00210... [more]
A0A5A7VA671.3e-6076.05Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold616G0011... [more]
A0A5D3DDU75.0e-6073.99Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1073G000... [more]
A0A5A7V6N06.5e-6066.50Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold749G0016... [more]
A0A5A7SNP86.5e-6066.00Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00216... [more]
Match NameE-valueIdentityDescription
KAA0063887.12.7e-6076.05gag/pol protein [Cucumis melo var. makuwa][more]
TYK15919.12.7e-6076.05gag/pol protein [Cucumis melo var. makuwa][more]
TYK21841.11.0e-5973.99gag/pol protein [Cucumis melo var. makuwa][more]
KAA0061339.11.3e-5966.50gag/pol protein [Cucumis melo var. makuwa][more]
KAA0026233.11.3e-5966.00gag/pol protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 23..147
e-value: 4.7E-12
score: 45.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 158..194
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..187
NoneNo IPR availablePANTHERPTHR35317:SF8POLYPROTEIN-LIKE PROTEINcoord: 12..179
NoneNo IPR availablePANTHERPTHR35317OS04G0629600 PROTEINcoord: 12..179

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005378.1PI0005378.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding