PI0005258 (gene) Melon (PI 482460) v1

BLAST of PI0005258 vs. ExPASy Swiss-Prot
Match: O22780 (Probable folate-biopterin transporter 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g33280 PE=3 SV=2)

HSP 1 Score: 566.6 bits (1459), Expect = 3.0e-160
Identity = 302/478 (63.18%), Postives = 372/478 (77.82%), Query Frame = 0

Query: 73  KKREGNGGRRGVSLVG---SNQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSLLQ 132
           +   G+G    V  VG     Q +LLC LGYWVQG RCF WLALNFHMAH LNL PS LQ
Sbjct: 62  RNNNGDGETTLVKKVGRDEKGQTVLLCALGYWVQGLRCFSWLALNFHMAHCLNLKPSTLQ 121

Query: 133 LVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALIPVAGEALPA 192
           LVQ + +LPMVAKPLYG+LSD  YIGGARRVPYIS+GVLLQ L+WGSLA+ P A E LP+
Sbjct: 122 LVQYTASLPMVAKPLYGVLSDVLYIGGARRVPYISVGVLLQGLAWGSLAIFPGAREVLPS 181

Query: 193 LMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGNFIGGYNLLS 252
           LMA +LLSNLGASITEV++DALVAEYG   +I GLQSY  MA AVGG+LGN +GGY LL 
Sbjct: 182 LMAFILLSNLGASITEVSQDALVAEYGLRYQINGLQSYALMASAVGGILGNLLGGYCLLK 241

Query: 253 SPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQISELVTVIS 312
           +PPR +FL F+ LLSLQL VSL+++EES+ L ++ E      S++  V+ Q  +L  ++ 
Sbjct: 242 TPPRILFLAFTALLSLQLIVSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQ 301

Query: 313 DPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDR 372
              +S+PL WIV+SIA+VPLLSGS+FCYQTQ LNLDPSVIGMS+V+ QL+LL +TV+YDR
Sbjct: 302 VDEISQPLIWIVSSIALVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDR 361

Query: 373 HWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLAETIAQFKLL 432
           +WK++PM+ LI +VQ+LYAFS L D +LVKQINL  GISN  F LCFS +AE +AQFK+L
Sbjct: 362 YWKKLPMRALIHIVQLLYAFSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKIL 421

Query: 433 PFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILI 492
           PFSVL A++ P GCEGS+ SF AS +CLSS+VSGF G+G+A++IGI S NYS+LP GILI
Sbjct: 422 PFSVLLANMCPGGCEGSITSFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILI 481

Query: 493 QFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCAYRRERES 548
           Q +AAL+PL  IH+VP   P  E+E KR MS++SR+NRRVGRV+   S  AYRRERE+
Sbjct: 482 QSLAALVPLWFIHYVPMLEPGFEREGKRAMSKKSRRNRRVGRVIGQESF-AYRRERET 538

BLAST of PI0005258 vs. ExPASy Swiss-Prot
Match: F4I5Q2 (Probable folate-biopterin transporter 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g04570 PE=2 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 9.8e-159
Identity = 312/525 (59.43%), Postives = 378/525 (72.00%), Query Frame = 0

Query: 30  FPKPILCFHHRNPNIFENPSKPIGIFIKTLLETNPDRVLFREVKKREGNGG------RRG 89
           F KP L   HR     E  S    +F+               +++R  N G         
Sbjct: 19  FLKPPLSSIHRQQQQQERQSNNNTLFMGEARPRRATTFACLSIRRRRNNNGVSEFEETAR 78

Query: 90  VSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSLLQLVQNSGNLPMVAKP 149
              VG   + +LCGLGYWVQG RCFPWLALNFHM H L L PS LQLVQ S +LPMVAKP
Sbjct: 79  FEQVGGKGISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSCSLPMVAKP 138

Query: 150 LYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSNLGASI 209
           LYG+LSD  YIG  RRVPYI+IGV LQVL+WGS+ +   A E LP+L+ACVLLSNLGASI
Sbjct: 139 LYGVLSDVLYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVLLSNLGASI 198

Query: 210 TEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLLFSILL 269
           TEVAKDALVAEYG   +I GLQSY  MA A GGVLGN +GGY LL++PP+  FL+FS LL
Sbjct: 199 TEVAKDALVAEYGLRYRINGLQSYALMASAAGGVLGNLLGGYLLLTTPPKISFLVFSALL 258

Query: 270 SLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQISELVTVISDPSVSRPLAWIVAS 329
           SLQL VSL+++EES GL ++ E      S++E+V+ QIS L   I    +S+PL W V S
Sbjct: 259 SLQLVVSLSSKEESFGLPRIAET----SSVLESVKKQISNLKEAIQADEISQPLIWAVVS 318

Query: 330 IAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMV 389
           IA+VPLLSGS+FCYQTQ LNLDPSVIGMS+V+ QL+LL +TV+YDR+ K +PM+ LI ++
Sbjct: 319 IAMVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLPMRPLIHII 378

Query: 390 QILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGC 449
           Q+LY  S LLD +LVKQINL  GISNE++ LCFS LAE +AQFK+LPF+V  AS+ P+GC
Sbjct: 379 QLLYGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRLASMCPQGC 438

Query: 450 EGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLTCIHF 509
           EGS+ SF AS +CLS IVS F G+GLA+LIGI S NYS+L  GILIQ +AAL PL  +H 
Sbjct: 439 EGSVTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAALAPLCFMHL 498

Query: 510 VPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCAYRRERESE 549
           VP + PV+EKE KR +S+RSR+NRRVGRV+   SV  YRRERESE
Sbjct: 499 VPMSEPVIEKEGKRGISKRSRRNRRVGRVVDKESV-TYRRERESE 538

BLAST of PI0005258 vs. ExPASy Swiss-Prot
Match: Q9XIQ7 (Probable folate-biopterin transporter 7 OS=Arabidopsis thaliana OX=3702 GN=At1g64890 PE=2 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 3.8e-86
Identity = 179/431 (41.53%), Postives = 271/431 (62.88%), Query Frame = 0

Query: 90  NQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSLLQLVQNSGNLPMVAKPLYGILS 149
           N +  L G G++VQGFR FPWL  NF +   L ++PS+LQL+QNS NLPMVAKP+YG++S
Sbjct: 17  NPIRWLLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVS 76

Query: 150 DAFYIGGARRVPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSNLGASITEVAKD 209
           D+ Y  G  R+PYI++G LLQ +SW ++A +  +  ++ AL   +LLSNLGAS+ EVA D
Sbjct: 77  DSVYFFGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVAND 136

Query: 210 ALVAEYGQ----TNKICGLQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLLFSILLSL 269
           A+VAE G+      +   L S+ +M  ++GG+LGN +GG  + +   +  FL+F IL  L
Sbjct: 137 AIVAESGKQKTSETQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFGILALL 196

Query: 270 QLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQISELVTVISDPSVSRPLAWIVASIA 329
           Q  V++  RE+SL L + P P          +R  +S+L  V+  P +S  +AWI  S A
Sbjct: 197 QFLVTINIREKSLNLPENPSP-------AGGIRKHLSDLSHVLRKPEISYSIAWIAVSTA 256

Query: 330 IVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQI 389
           +VP+L+G++F YQT+ L +D S++G+S+V  Q+ +L     Y+R  K +  +KL+  +Q+
Sbjct: 257 VVPVLTGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQV 316

Query: 390 LYAFSFLLDIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEG 449
             AF  + D++ VK +   LG+S+ ++ L FSG  ET+  FK+LPF+VL A L P GCEG
Sbjct: 317 TIAFFVISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEG 376

Query: 450 SLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLTCIHFVP 509
           SL++F  SA+ L+ IVSG+ GI LAS +G+   ++S    G+ I+     +PL    ++ 
Sbjct: 377 SLMAFVMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIY 436

Query: 510 TTSPVLEKEKK 517
             +   EK KK
Sbjct: 437 DEAETKEKSKK 440

BLAST of PI0005258 vs. ExPASy Swiss-Prot
Match: Q8RWQ5 (Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g54860 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.5e-53
Identity = 154/476 (32.35%), Postives = 234/476 (49.16%), Query Frame = 0

Query: 85  SLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSLLQLVQNSGNLPMVAKPL 144
           S  G   + L+C L Y+ QGFR F W A+++ +   L L PS  Q V +    P   KPL
Sbjct: 10  SAFGVAFLWLVC-LIYFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPL 69

Query: 145 YGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSNLGASIT 204
           YGI+SD   IGG +R PY+ I  +L ++ W  L L   +  +   LM  + + NLG+++ 
Sbjct: 70  YGIISDCIPIGGKKRTPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMA 129

Query: 205 EVAKDALVAEYGQTNKIC---GLQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLLFSI 264
           +V  DA++AE  +  K      LQS ++ A+AVGGV G+ +GGY L +     +FLLF++
Sbjct: 130 DVVIDAMIAEAVRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTV 189

Query: 265 LLSLQLSVSLATREESLGLLQLPE------------------------------------ 324
           L +LQL       E       LPE                                    
Sbjct: 190 LPALQLLSCALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGK 249

Query: 325 -PDVHGKSIMENVRNQISELVT---------------VISDPSVSRPLAWIVASIAIVPL 384
             D +GKS  E  + Q   L +                   P + RP+AW   +   VP 
Sbjct: 250 KGDSNGKS--ETQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPN 309

Query: 385 LSGSIFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQILYAF 444
           LS  +F YQT+ L LD + +G +RVV  L L+  T +Y+R+ + + ++K +    I  + 
Sbjct: 310 LSTVMFYYQTEVLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSV 369

Query: 445 SFLLDIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLIS 504
           + LLD+VLV + N+  G+S++   L  S L + I Q K +PF +L   L P G EG+L +
Sbjct: 370 TILLDMVLVSRANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFA 429

Query: 505 FFASAVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLTCIHFVP 506
            F S   L + V  F G GLASL+GI SG++ ++ +G+ IQ     +P+  +  +P
Sbjct: 430 LFMSINNLGNTVGSFMGAGLASLLGISSGSFDNMFMGLAIQVFCTYIPVLFLFLIP 482

BLAST of PI0005258 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 5.9e-31
Identity = 113/416 (27.16%), Postives = 205/416 (49.28%), Query Frame = 0

Query: 100 YWVQGFRCFPWLALNFHMAHYLNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARR 159
           Y+VQG      LA++F++   L+L P+   ++    +LP + KPLYG +SD+  + G RR
Sbjct: 134 YFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFISDSVPLFGYRR 193

Query: 160 VPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSNLGASITEVAKDALVAE--YGQ 219
             Y+ +  LL   SW  +A      ++  +   C+LL +L  + ++V  D++V E   G+
Sbjct: 194 RSYLVLSGLLGAFSWSLMAGFV---DSKYSAAFCILLGSLSVAFSDVVVDSMVVERARGE 253

Query: 220 TNKICG-LQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREE 279
           +  + G LQS  + + A GG++ ++  G  + S   R +F + ++L  +  +V++   E+
Sbjct: 254 SQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTALLPLITSAVAVLVNEQ 313

Query: 280 SL---GLLQLPEPDVHGKSIMENVRNQISELVTVISDPSVSRPLAWIVASIAIVPLLSGS 339
            +      Q     +     ++  +  + +L   I  P+V  P  +I    A  P    +
Sbjct: 314 RVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPTLFIFLWQA-TPHSDSA 373

Query: 340 IFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLL 399
           +F + T  L   P  +G  ++V+ +  L    LY+   K VP++K+  +  I      + 
Sbjct: 374 MFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLRKIFLVTTIFGTGLGMT 433

Query: 400 DIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFAS 459
            ++LV   N +LGIS+E FA+  S +   +AQ   +P  VL A L P+G E +L +   S
Sbjct: 434 QVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAARLCPEGMEATLFATLMS 493

Query: 460 AVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLTCIHFVPTTSP 510
                S++ G  G GL    GI   ++ +L   I++  +++LLPL  +  +P  SP
Sbjct: 494 ISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLPLPLLGLLPRDSP 545

BLAST of PI0005258 vs. ExPASy TrEMBL
Match: A0A1S3AX05 (probable folate-biopterin transporter 9, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483535 PE=3 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 2.7e-297
Identity = 536/551 (97.28%), Postives = 545/551 (98.91%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MISSLISPNFPFPSLKS S+RPPPKPSLSFPKPILCFHHRNPNIFEN SKPIGIFIKT+L
Sbjct: 1   MISSLISPNFPFPSLKSNSSRPPPKPSLSFPKPILCFHHRNPNIFENSSKPIGIFIKTIL 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+NPDRVLFREVKKREGNGG+RGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPDRVLFREVKKREGNGGKRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPSLLQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPYISIGVLLQVLSWGSLALI
Sbjct: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYISIGVLLQVLSWGSLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGL+QLPEPDVHGKSIMENVRNQ
Sbjct: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLVQLPEPDVHGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPS+IGMSRVVSQLVL
Sbjct: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRHWKRVPMKKLIGMVQILY+FSFLLDIVLVKQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYSFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGF GIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFLGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGILIQFIAALLPLTCIHFVP  SPVLE+EKKRVMSRRSRKNRRVGRVMYSGSVCA
Sbjct: 481 SSLPVGILIQFIAALLPLTCIHFVPMKSPVLEREKKRVMSRRSRKNRRVGRVMYSGSVCA 540

Query: 541 YRRERESEMQG 552
           YRRERESE+QG
Sbjct: 541 YRRERESEIQG 551

BLAST of PI0005258 vs. ExPASy TrEMBL
Match: A0A0A0L6X9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G133990 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.8e-286
Identity = 527/560 (94.11%), Postives = 535/560 (95.54%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MISSLISPNFPFPSLKS  AR  PK SLSF KP+LCFHHRNPNIFENPSKPI IFIKT+L
Sbjct: 1   MISSLISPNFPFPSLKSNFAR--PKTSLSFHKPVLCFHHRNPNIFENPSKPIAIFIKTVL 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+NPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQ---------V 180
           LNLHPSLLQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPYISIGV L+         V
Sbjct: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYISIGVGLRIQTLHLQRVV 180

Query: 181 LSWGSLALIPVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMA 240
           LSWGSLALIPVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMA
Sbjct: 181 LSWGSLALIPVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMA 240

Query: 241 LAVGGVLGNFIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGK 300
           LAVGGVLGNFIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDV GK
Sbjct: 241 LAVGGVLGNFIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVQGK 300

Query: 301 SIMENVRNQISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGM 360
           SIMENVRNQISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPS+IGM
Sbjct: 301 SIMENVRNQISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSIIGM 360

Query: 361 SRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNEL 420
           SRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQILYA SFLLDIVL+KQINLKLGISNEL
Sbjct: 361 SRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQILYALSFLLDIVLMKQINLKLGISNEL 420

Query: 421 FALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLAS 480
           FALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGF GIGLAS
Sbjct: 421 FALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFLGIGLAS 480

Query: 481 LIGIRSGNYSSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGR 540
           LIGI SGNYSSLPVGILIQFIAAL PLTCIHFVP  SPVLE+EKKRVMSRRSRKNRRVGR
Sbjct: 481 LIGIGSGNYSSLPVGILIQFIAALFPLTCIHFVPMKSPVLEREKKRVMSRRSRKNRRVGR 540

Query: 541 VMYSGSVCAYRRERESEMQG 552
           VMYSGSVCAYRRERESE+QG
Sbjct: 541 VMYSGSVCAYRRERESEIQG 558

BLAST of PI0005258 vs. ExPASy TrEMBL
Match: A0A6J1EA28 (probable folate-biopterin transporter 9, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111432152 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.7e-270
Identity = 489/551 (88.75%), Postives = 515/551 (93.47%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MI SL +P+FPFPSLK    R P +P L FPK ILC HHRNPNIFENPSKP GIFI TL+
Sbjct: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+NP  VL+RE KKREGNG +R   LVGS QMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPS+LQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPY+SIGVLLQVLSWG LALI
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAE+GQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           F+GGYNLLSSPPRKMFLLFSILLSLQLS+S ATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           +S+LVT I DPS+ RPLAWIVASIA+VPLL+GSIFCYQTQCL+LDPS+IGMSRVVSQLVL
Sbjct: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTVLYDR+WKRV MKKLIGMVQILYA SFLLDIVLV QINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIV GFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGILIQFIAALLPL CIHFVPTTSPV+E+EKKR++SRRSR+NRRVGRV+YS SV  
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTTSPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

Query: 541 YRRERESEMQG 552
           YRRERESEMQG
Sbjct: 541 YRRERESEMQG 551

BLAST of PI0005258 vs. ExPASy TrEMBL
Match: A0A6J1IYT4 (probable folate-biopterin transporter 9, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479684 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.4e-269
Identity = 487/551 (88.38%), Postives = 515/551 (93.47%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MI SLI+P+FPFPSLK    R P +P L FPK ILC HHRNPNIFENPSKP GIFI TL+
Sbjct: 1   MICSLIAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+NP  VL+REVKKREGNG +R   LVGS QMLL+CGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREVKKREGNGRKREFPLVGSYQMLLICGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPS+LQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPY+SIGVLLQVLSWG LALI
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAE+GQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           F+GGYNLLSSPPRKMFLLFSILLSLQLS+S ATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           +S+L+T I DPS+ RPLAWIVASIA+VPLL+GSIFCYQTQCL+LDPS+IGMSRVVSQLVL
Sbjct: 301 MSDLITAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTVLYDR+WKRV MKKLIGMVQILYA SFLLDIVLV QINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIV GFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGILIQFIAALLPL CIHFVPT SP +E+EKKR++SRRSR+NRRVGRV+YS SV  
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTISPAVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

Query: 541 YRRERESEMQG 552
           YRRERESEMQG
Sbjct: 541 YRRERESEMQG 551

BLAST of PI0005258 vs. ExPASy TrEMBL
Match: A0A6J1DZL8 (probable folate-biopterin transporter 9, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111024552 PE=3 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.6e-263
Identity = 483/550 (87.82%), Postives = 507/550 (92.18%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MI SLI+P  PFPSLK   ARPP       PKPILC HHRNPN  + PSKPIGIF  TLL
Sbjct: 1   MICSLIAPGNPFPSLKPIFARPP-------PKPILCLHHRNPNSIQTPSKPIGIFTNTLL 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           + NP R L+RE +KREGNG RRG SLVGS++MLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  QNNPARGLYREGEKREGNGRRRGSSLVGSHRMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPS LQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPY+SIGVLLQVLSWGSLALI
Sbjct: 121 LNLHPSTLQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGSLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQT+KICGLQSYTFM LAVGG+LGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTHKICGLQSYTFMGLAVGGILGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FIGGYNLLSSPPRKMFLLFSILLSLQLS+S ATREESLGLLQL EPDVHGKSIMENVRNQ
Sbjct: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLSEPDVHGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           I++LVT +SD SV  PL WIVASIA+VP+LSGSIFCYQTQCL+LDPS+IGMSRVVSQLVL
Sbjct: 301 INDLVTAVSDQSVFLPLTWIVASIAMVPVLSGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRHWKRV MKKLIGMVQILYA S L D+VLVKQINLKLGISNE+FALCFSGLA
Sbjct: 361 LSVTVLYDRHWKRVSMKKLIGMVQILYASSLLFDLVLVKQINLKLGISNEMFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSV+FA+LSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVIFANLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGILIQFIAALLPL  IHFVPTTSPV+E+EKKRVMSRRSRKNRRVGRVMYSGSV  
Sbjct: 481 SSLPVGILIQFIAALLPLIWIHFVPTTSPVVEREKKRVMSRRSRKNRRVGRVMYSGSVYV 540

Query: 541 YRRERESEMQ 551
           YRRERESEMQ
Sbjct: 541 YRRERESEMQ 543

BLAST of PI0005258 vs. NCBI nr
Match: XP_008438439.1 (PREDICTED: probable folate-biopterin transporter 9, chloroplastic [Cucumis melo])

HSP 1 Score: 1030.4 bits (2663), Expect = 5.7e-297
Identity = 536/551 (97.28%), Postives = 545/551 (98.91%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MISSLISPNFPFPSLKS S+RPPPKPSLSFPKPILCFHHRNPNIFEN SKPIGIFIKT+L
Sbjct: 1   MISSLISPNFPFPSLKSNSSRPPPKPSLSFPKPILCFHHRNPNIFENSSKPIGIFIKTIL 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+NPDRVLFREVKKREGNGG+RGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPDRVLFREVKKREGNGGKRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPSLLQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPYISIGVLLQVLSWGSLALI
Sbjct: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYISIGVLLQVLSWGSLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGL+QLPEPDVHGKSIMENVRNQ
Sbjct: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLVQLPEPDVHGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPS+IGMSRVVSQLVL
Sbjct: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRHWKRVPMKKLIGMVQILY+FSFLLDIVLVKQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYSFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGF GIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFLGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGILIQFIAALLPLTCIHFVP  SPVLE+EKKRVMSRRSRKNRRVGRVMYSGSVCA
Sbjct: 481 SSLPVGILIQFIAALLPLTCIHFVPMKSPVLEREKKRVMSRRSRKNRRVGRVMYSGSVCA 540

Query: 541 YRRERESEMQG 552
           YRRERESE+QG
Sbjct: 541 YRRERESEIQG 551

BLAST of PI0005258 vs. NCBI nr
Match: XP_004134394.2 (probable folate-biopterin transporter 9, chloroplastic isoform X1 [Cucumis sativus] >KAE8650320.1 hypothetical protein Csa_009743 [Cucumis sativus])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.1e-289
Identity = 529/551 (96.01%), Postives = 536/551 (97.28%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MISSLISPNFPFPSLKS  AR  PK SLSF KP+LCFHHRNPNIFENPSKPI IFIKT+L
Sbjct: 1   MISSLISPNFPFPSLKSNFAR--PKTSLSFHKPVLCFHHRNPNIFENPSKPIAIFIKTVL 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+NPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPSLLQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPYISIGVLLQVLSWGSLALI
Sbjct: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYISIGVLLQVLSWGSLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDV GKSIMENVRNQ
Sbjct: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVQGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPS+IGMSRVVSQLVL
Sbjct: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTVLYDRHWKRVPMKKLIGMVQILYA SFLLDIVL+KQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYALSFLLDIVLMKQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGF GIGLASLIGI SGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFLGIGLASLIGIGSGNY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGILIQFIAAL PLTCIHFVP  SPVLE+EKKRVMSRRSRKNRRVGRVMYSGSVCA
Sbjct: 481 SSLPVGILIQFIAALFPLTCIHFVPMKSPVLEREKKRVMSRRSRKNRRVGRVMYSGSVCA 540

Query: 541 YRRERESEMQG 552
           YRRERESE+QG
Sbjct: 541 YRRERESEIQG 549

BLAST of PI0005258 vs. NCBI nr
Match: XP_038882771.1 (probable folate-biopterin transporter 8, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 971.1 bits (2509), Expect = 4.1e-279
Identity = 505/551 (91.65%), Postives = 525/551 (95.28%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MI SLI+P+FPFPSLK   ARPPPKPSLSFPKPILCFHHRNPNIFENPSKP+GIFIKTL+
Sbjct: 1   MICSLITPDFPFPSLKPLFARPPPKPSLSFPKPILCFHHRNPNIFENPSKPVGIFIKTLV 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+N DRVL+REVKKREGNGGRRG +LVGS+QMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNSDRVLYREVKKREGNGGRRGFALVGSHQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPS+LQLVQNSGN PMVAKPLYGILSDA YIGGARRVPY+SIGVLLQVLSWGSLALI
Sbjct: 121 LNLHPSILQLVQNSGNFPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGSLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           FIGGYNL SSPPRKMFLLFSI+L  QLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FIGGYNLSSSPPRKMFLLFSIILCFQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           IS+LVT ISDPSV RPLAW+VASI++VPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL
Sbjct: 301 ISDLVTSISDPSVYRPLAWVVASISVVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTV YD HWKRV M+KLIGMVQILYA SFLLDIVLVKQINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVFYDHHWKRVSMRKLIGMVQILYASSFLLDIVLVKQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIV GFFGIGLASLIGIRSG+Y
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGDY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGI IQFIAALLPL  IHFVP  SPV+E+EKKR MSRRSRKNRRVGRVMYSGSV  
Sbjct: 481 SSLPVGISIQFIAALLPLMWIHFVPKMSPVVEREKKRGMSRRSRKNRRVGRVMYSGSVYV 540

Query: 541 YRRERESEMQG 552
           YRRERESE+QG
Sbjct: 541 YRRERESEVQG 551

BLAST of PI0005258 vs. NCBI nr
Match: XP_023528713.1 (probable folate-biopterin transporter 9, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941.8 bits (2433), Expect = 2.6e-270
Identity = 490/551 (88.93%), Postives = 516/551 (93.65%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MI SLI+P+FPFPSLK    R P +P L FPK ILC HHRNPNIFENPSKP GIFI TL+
Sbjct: 1   MICSLIAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+NP  VL+RE KKREGNG +R   LVGS QMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPS+LQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPY+SIGVLLQVLSWG LALI
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAE+GQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           F+GGYNLLSSPPRKMFLLFSILLSLQLS+S ATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           +S+LVT I DPS+ RPLAWIVASIA+VPLL+GSIFCYQTQCL+LDPS+IGMSRVVSQLVL
Sbjct: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTVLYDR+WKRV MKKLIGMVQILYA SFLLDIVLV QINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIV GFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGILIQFIAALLPL CIHFVPTTSPV+E+EKKR++SRRSR+NRRVGRV+YS SV  
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTTSPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

Query: 541 YRRERESEMQG 552
           YRRERESEMQG
Sbjct: 541 YRRERESEMQG 551

BLAST of PI0005258 vs. NCBI nr
Match: XP_022924744.1 (probable folate-biopterin transporter 9, chloroplastic [Cucurbita moschata])

HSP 1 Score: 940.3 bits (2429), Expect = 7.7e-270
Identity = 489/551 (88.75%), Postives = 515/551 (93.47%), Query Frame = 0

Query: 1   MISSLISPNFPFPSLKSKSARPPPKPSLSFPKPILCFHHRNPNIFENPSKPIGIFIKTLL 60
           MI SL +P+FPFPSLK    R P +P L FPK ILC HHRNPNIFENPSKP GIFI TL+
Sbjct: 1   MICSLFAPDFPFPSLKPTFPRSPRRPPLYFPKHILCLHHRNPNIFENPSKPTGIFINTLV 60

Query: 61  ETNPDRVLFREVKKREGNGGRRGVSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120
           E+NP  VL+RE KKREGNG +R   LVGS QMLLLCGLGYWVQGFRCFPWLALNFHMAHY
Sbjct: 61  ESNPGHVLYREAKKREGNGRKREFPLVGSYQMLLLCGLGYWVQGFRCFPWLALNFHMAHY 120

Query: 121 LNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALI 180
           LNLHPS+LQLVQNSGNLPMVAKPLYGILSDA YIGGARRVPY+SIGVLLQVLSWG LALI
Sbjct: 121 LNLHPSILQLVQNSGNLPMVAKPLYGILSDALYIGGARRVPYVSIGVLLQVLSWGFLALI 180

Query: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGN 240
           PVAGEALPALMACVLLSNLGASITEVAKDALVAE+GQTNKICGLQSYTFMALAVGGVLGN
Sbjct: 181 PVAGEALPALMACVLLSNLGASITEVAKDALVAEFGQTNKICGLQSYTFMALAVGGVLGN 240

Query: 241 FIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQ 300
           F+GGYNLLSSPPRKMFLLFSILLSLQLS+S ATREESLGLLQLPEPDVHGKSIMENVRNQ
Sbjct: 241 FLGGYNLLSSPPRKMFLLFSILLSLQLSISSATREESLGLLQLPEPDVHGKSIMENVRNQ 300

Query: 301 ISELVTVISDPSVSRPLAWIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVL 360
           +S+LVT I DPS+ RPLAWIVASIA+VPLL+GSIFCYQTQCL+LDPS+IGMSRVVSQLVL
Sbjct: 301 MSDLVTAIRDPSIFRPLAWIVASIAMVPLLTGSIFCYQTQCLHLDPSIIGMSRVVSQLVL 360

Query: 361 LSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLA 420
           LSVTVLYDR+WKRV MKKLIGMVQILYA SFLLDIVLV QINLKLGISNELFALCFSGLA
Sbjct: 361 LSVTVLYDRYWKRVSMKKLIGMVQILYASSFLLDIVLVNQINLKLGISNELFALCFSGLA 420

Query: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNY 480
           ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIV GFFGIGLASLIGIRSGNY
Sbjct: 421 ETIAQFKLLPFSVLFASLSPKGCEGSLISFFASAVCLSSIVGGFFGIGLASLIGIRSGNY 480

Query: 481 SSLPVGILIQFIAALLPLTCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCA 540
           SSLPVGILIQFIAALLPL CIHFVPTTSPV+E+EKKR++SRRSR+NRRVGRV+YS SV  
Sbjct: 481 SSLPVGILIQFIAALLPLICIHFVPTTSPVVEREKKRIVSRRSRRNRRVGRVVYSDSVYV 540

Query: 541 YRRERESEMQG 552
           YRRERESEMQG
Sbjct: 541 YRRERESEMQG 551

BLAST of PI0005258 vs. TAIR 10
Match: AT1G04570.1 (Major facilitator superfamily protein )

HSP 1 Score: 561.6 bits (1446), Expect = 6.9e-160
Identity = 312/525 (59.43%), Postives = 378/525 (72.00%), Query Frame = 0

Query: 30  FPKPILCFHHRNPNIFENPSKPIGIFIKTLLETNPDRVLFREVKKREGNGG------RRG 89
           F KP L   HR     E  S    +F+               +++R  N G         
Sbjct: 19  FLKPPLSSIHRQQQQQERQSNNNTLFMGEARPRRATTFACLSIRRRRNNNGVSEFEETAR 78

Query: 90  VSLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSLLQLVQNSGNLPMVAKP 149
              VG   + +LCGLGYWVQG RCFPWLALNFHM H L L PS LQLVQ S +LPMVAKP
Sbjct: 79  FEQVGGKGISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSCSLPMVAKP 138

Query: 150 LYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSNLGASI 209
           LYG+LSD  YIG  RRVPYI+IGV LQVL+WGS+ +   A E LP+L+ACVLLSNLGASI
Sbjct: 139 LYGVLSDVLYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVLLSNLGASI 198

Query: 210 TEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLLFSILL 269
           TEVAKDALVAEYG   +I GLQSY  MA A GGVLGN +GGY LL++PP+  FL+FS LL
Sbjct: 199 TEVAKDALVAEYGLRYRINGLQSYALMASAAGGVLGNLLGGYLLLTTPPKISFLVFSALL 258

Query: 270 SLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQISELVTVISDPSVSRPLAWIVAS 329
           SLQL VSL+++EES GL ++ E      S++E+V+ QIS L   I    +S+PL W V S
Sbjct: 259 SLQLVVSLSSKEESFGLPRIAET----SSVLESVKKQISNLKEAIQADEISQPLIWAVVS 318

Query: 330 IAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMV 389
           IA+VPLLSGS+FCYQTQ LNLDPSVIGMS+V+ QL+LL +TV+YDR+ K +PM+ LI ++
Sbjct: 319 IAMVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLPMRPLIHII 378

Query: 390 QILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGC 449
           Q+LY  S LLD +LVKQINL  GISNE++ LCFS LAE +AQFK+LPF+V  AS+ P+GC
Sbjct: 379 QLLYGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRLASMCPQGC 438

Query: 450 EGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLTCIHF 509
           EGS+ SF AS +CLS IVS F G+GLA+LIGI S NYS+L  GILIQ +AAL PL  +H 
Sbjct: 439 EGSVTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAALAPLCFMHL 498

Query: 510 VPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCAYRRERESE 549
           VP + PV+EKE KR +S+RSR+NRRVGRV+   SV  YRRERESE
Sbjct: 499 VPMSEPVIEKEGKRGISKRSRRNRRVGRVVDKESV-TYRRERESE 538

BLAST of PI0005258 vs. TAIR 10
Match: AT2G33280.1 (Major facilitator superfamily protein )

HSP 1 Score: 490.0 bits (1260), Expect = 2.6e-138
Identity = 261/409 (63.81%), Postives = 326/409 (79.71%), Query Frame = 0

Query: 139 MVAKPLYGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSN 198
           MVAKPLYG+LSD  YIGGARRVPYIS+GVLLQ L+WGSLA+ P A E LP+LMA +LLSN
Sbjct: 1   MVAKPLYGVLSDVLYIGGARRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSN 60

Query: 199 LGASITEVAKDALVAEYGQTNKICGLQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLL 258
           LGASITEV++DALVAEYG   +I GLQSY  MA AVGG+LGN +GGY LL +PPR +FL 
Sbjct: 61  LGASITEVSQDALVAEYGLRYQINGLQSYALMASAVGGILGNLLGGYCLLKTPPRILFLA 120

Query: 259 FSILLSLQLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQISELVTVISDPSVSRPLA 318
           F+ LLSLQL VSL+++EES+ L ++ E      S++  V+ Q  +L  ++    +S+PL 
Sbjct: 121 FTALLSLQLIVSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQVDEISQPLI 180

Query: 319 WIVASIAIVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKK 378
           WIV+SIA+VPLLSGS+FCYQTQ LNLDPSVIGMS+V+ QL+LL +TV+YDR+WK++PM+ 
Sbjct: 181 WIVSSIALVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYWKKLPMRA 240

Query: 379 LIGMVQILYAFSFLLDIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASL 438
           LI +VQ+LYAFS L D +LVKQINL  GISN  F LCFS +AE +AQFK+LPFSVL A++
Sbjct: 241 LIHIVQLLYAFSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKILPFSVLLANM 300

Query: 439 SPKGCEGSLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPL 498
            P GCEGS+ SF AS +CLSS+VSGF G+G+A++IGI S NYS+LP GILIQ +AAL+PL
Sbjct: 301 CPGGCEGSITSFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILIQSLAALVPL 360

Query: 499 TCIHFVPTTSPVLEKEKKRVMSRRSRKNRRVGRVMYSGSVCAYRRERES 548
             IH+VP   P  E+E KR MS++SR+NRRVGRV+   S  AYRRERE+
Sbjct: 361 WFIHYVPMLEPGFEREGKRAMSKKSRRNRRVGRVIGQESF-AYRRERET 408

BLAST of PI0005258 vs. TAIR 10
Match: AT1G64890.1 (Major facilitator superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 2.7e-87
Identity = 179/431 (41.53%), Postives = 271/431 (62.88%), Query Frame = 0

Query: 90  NQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSLLQLVQNSGNLPMVAKPLYGILS 149
           N +  L G G++VQGFR FPWL  NF +   L ++PS+LQL+QNS NLPMVAKP+YG++S
Sbjct: 17  NPIRWLLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVS 76

Query: 150 DAFYIGGARRVPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSNLGASITEVAKD 209
           D+ Y  G  R+PYI++G LLQ +SW ++A +  +  ++ AL   +LLSNLGAS+ EVA D
Sbjct: 77  DSVYFFGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVAND 136

Query: 210 ALVAEYGQ----TNKICGLQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLLFSILLSL 269
           A+VAE G+      +   L S+ +M  ++GG+LGN +GG  + +   +  FL+F IL  L
Sbjct: 137 AIVAESGKQKTSETQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFGILALL 196

Query: 270 QLSVSLATREESLGLLQLPEPDVHGKSIMENVRNQISELVTVISDPSVSRPLAWIVASIA 329
           Q  V++  RE+SL L + P P          +R  +S+L  V+  P +S  +AWI  S A
Sbjct: 197 QFLVTINIREKSLNLPENPSP-------AGGIRKHLSDLSHVLRKPEISYSIAWIAVSTA 256

Query: 330 IVPLLSGSIFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQI 389
           +VP+L+G++F YQT+ L +D S++G+S+V  Q+ +L     Y+R  K +  +KL+  +Q+
Sbjct: 257 VVPVLTGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQV 316

Query: 390 LYAFSFLLDIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEG 449
             AF  + D++ VK +   LG+S+ ++ L FSG  ET+  FK+LPF+VL A L P GCEG
Sbjct: 317 TIAFFVISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEG 376

Query: 450 SLISFFASAVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLTCIHFVP 509
           SL++F  SA+ L+ IVSG+ GI LAS +G+   ++S    G+ I+     +PL    ++ 
Sbjct: 377 SLMAFVMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIY 436

Query: 510 TTSPVLEKEKK 517
             +   EK KK
Sbjct: 437 DEAETKEKSKK 440

BLAST of PI0005258 vs. TAIR 10
Match: AT5G54860.1 (Major facilitator superfamily protein )

HSP 1 Score: 211.5 bits (537), Expect = 1.8e-54
Identity = 154/476 (32.35%), Postives = 234/476 (49.16%), Query Frame = 0

Query: 85  SLVGSNQMLLLCGLGYWVQGFRCFPWLALNFHMAHYLNLHPSLLQLVQNSGNLPMVAKPL 144
           S  G   + L+C L Y+ QGFR F W A+++ +   L L PS  Q V +    P   KPL
Sbjct: 10  SAFGVAFLWLVC-LIYFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPL 69

Query: 145 YGILSDAFYIGGARRVPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSNLGASIT 204
           YGI+SD   IGG +R PY+ I  +L ++ W  L L   +  +   LM  + + NLG+++ 
Sbjct: 70  YGIISDCIPIGGKKRTPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMA 129

Query: 205 EVAKDALVAEYGQTNKIC---GLQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLLFSI 264
           +V  DA++AE  +  K      LQS ++ A+AVGGV G+ +GGY L +     +FLLF++
Sbjct: 130 DVVIDAMIAEAVRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTV 189

Query: 265 LLSLQLSVSLATREESLGLLQLPE------------------------------------ 324
           L +LQL       E       LPE                                    
Sbjct: 190 LPALQLLSCALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGK 249

Query: 325 -PDVHGKSIMENVRNQISELVT---------------VISDPSVSRPLAWIVASIAIVPL 384
             D +GKS  E  + Q   L +                   P + RP+AW   +   VP 
Sbjct: 250 KGDSNGKS--ETQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPN 309

Query: 385 LSGSIFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQILYAF 444
           LS  +F YQT+ L LD + +G +RVV  L L+  T +Y+R+ + + ++K +    I  + 
Sbjct: 310 LSTVMFYYQTEVLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSV 369

Query: 445 SFLLDIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLIS 504
           + LLD+VLV + N+  G+S++   L  S L + I Q K +PF +L   L P G EG+L +
Sbjct: 370 TILLDMVLVSRANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFA 429

Query: 505 FFASAVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLTCIHFVP 506
            F S   L + V  F G GLASL+GI SG++ ++ +G+ IQ     +P+  +  +P
Sbjct: 430 LFMSINNLGNTVGSFMGAGLASLLGISSGSFDNMFMGLAIQVFCTYIPVLFLFLIP 482

BLAST of PI0005258 vs. TAIR 10
Match: AT2G32040.1 (Major facilitator superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 4.2e-32
Identity = 113/416 (27.16%), Postives = 205/416 (49.28%), Query Frame = 0

Query: 100 YWVQGFRCFPWLALNFHMAHYLNLHPSLLQLVQNSGNLPMVAKPLYGILSDAFYIGGARR 159
           Y+VQG      LA++F++   L+L P+   ++    +LP + KPLYG +SD+  + G RR
Sbjct: 134 YFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFISDSVPLFGYRR 193

Query: 160 VPYISIGVLLQVLSWGSLALIPVAGEALPALMACVLLSNLGASITEVAKDALVAE--YGQ 219
             Y+ +  LL   SW  +A      ++  +   C+LL +L  + ++V  D++V E   G+
Sbjct: 194 RSYLVLSGLLGAFSWSLMAGFV---DSKYSAAFCILLGSLSVAFSDVVVDSMVVERARGE 253

Query: 220 TNKICG-LQSYTFMALAVGGVLGNFIGGYNLLSSPPRKMFLLFSILLSLQLSVSLATREE 279
           +  + G LQS  + + A GG++ ++  G  + S   R +F + ++L  +  +V++   E+
Sbjct: 254 SQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTALLPLITSAVAVLVNEQ 313

Query: 280 SL---GLLQLPEPDVHGKSIMENVRNQISELVTVISDPSVSRPLAWIVASIAIVPLLSGS 339
            +      Q     +     ++  +  + +L   I  P+V  P  +I    A  P    +
Sbjct: 314 RVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPTLFIFLWQA-TPHSDSA 373

Query: 340 IFCYQTQCLNLDPSVIGMSRVVSQLVLLSVTVLYDRHWKRVPMKKLIGMVQILYAFSFLL 399
           +F + T  L   P  +G  ++V+ +  L    LY+   K VP++K+  +  I      + 
Sbjct: 374 MFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLRKIFLVTTIFGTGLGMT 433

Query: 400 DIVLVKQINLKLGISNELFALCFSGLAETIAQFKLLPFSVLFASLSPKGCEGSLISFFAS 459
            ++LV   N +LGIS+E FA+  S +   +AQ   +P  VL A L P+G E +L +   S
Sbjct: 434 QVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAARLCPEGMEATLFATLMS 493

Query: 460 AVCLSSIVSGFFGIGLASLIGIRSGNYSSLPVGILIQFIAALLPLTCIHFVPTTSP 510
                S++ G  G GL    GI   ++ +L   I++  +++LLPL  +  +P  SP
Sbjct: 494 ISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLPLPLLGLLPRDSP 545