Homology
BLAST of PI0005071 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 493.4 bits (1269), Expect = 3.1e-138
Identity = 258/517 (49.90%), Postives = 355/517 (68.67%), Query Frame = 0
Query: 4 QTSFFFFFFSHFHPISIPLYLFPIFLTTLFLISKFRTTHHSKLPPTPP-SLPIIGHLHLL 63
++S + F +SI L LFP+ + LPP+P +LP+IGHLHL+
Sbjct: 2 ESSTLLYTFLAVVLLSISLKLFPV------------SRRRRNLPPSPGLALPVIGHLHLI 61
Query: 64 KEPFHQMLQDLSQKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKIL 123
+ H+ L DLS+KYG VFSL LG R ++VVSSP+A +ECFTKNDI+FA+RP + GK +
Sbjct: 62 GKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYI 121
Query: 124 NYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKN 183
Y+YT + + YG+HWR +RR+A E+ S LN IRE+EVK + LY + ++
Sbjct: 122 GYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLY--QSSGQD 181
Query: 184 GARVGLRSKLTELSFNIALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPAD 243
+V ++SKL+ELSFN+ + ++AG+RYFG++V +S+EAK + + +V +GAS P D
Sbjct: 182 FGKVEMKSKLSELSFNVTMRMVAGKRYFGQDV--DSDEAKLFRALIGEVFEHAGASNPGD 241
Query: 244 FLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPE 303
F+ L+ IDF+ + K+++K+ + DAF Q L+ E R K + TMID +L LQE+ PE
Sbjct: 242 FVPFLRWIDFKNYEKKVSKISQEMDAFLQRLIHESRINKNNV---TMIDHLLSLQESQPE 301
Query: 304 YYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEA 363
YY D IIKG + LL AGTDT+A T+EWAMSLLLNHP +++KA TEI VG RL++E
Sbjct: 302 YYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQ 361
Query: 364 DTNKLHYLQAIINETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNV 423
D KL YL II+ET+RL A P+LVP ESS+DC + G+++PKGT+L+VNAWA+HRDP
Sbjct: 362 DLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEF 421
Query: 424 WEHPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLD 483
W+ P FKPER G +E SKL+PFGMGRR+CPG+GLA RVV L L LIQCFEW ++
Sbjct: 422 WDEPTLFKPER-HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIG 481
Query: 484 DQKEVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQ 520
+ K +D+ G GLTMPKA PLEA+CKPR + ++S+
Sbjct: 482 EAK-IDMAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497
BLAST of PI0005071 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 458.4 bits (1178), Expect = 1.1e-127
Identity = 233/504 (46.23%), Postives = 334/504 (66.27%), Query Frame = 0
Query: 20 IPLYLFPIFLTTLFLISK--FRTTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLSQKY 79
+ L + +F LF I R LPP PPSLPIIG+LH LK P H+ + LS+KY
Sbjct: 4 LSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKY 63
Query: 80 GPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAYGQH 139
G VFSL G R +VVVSS S Q+CFTKND++ A+RPR LSGK + Y+YT +G+T+YG+H
Sbjct: 64 GHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEH 123
Query: 140 WRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTELSF 199
WR +RRI ++LS R+N S IR +E + + RL ++ N A + L S+L +++F
Sbjct: 124 WRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSS--TNFAEMELSSRLYDMTF 183
Query: 200 NIALMLMAGRRYFGEEVE-GESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFR 259
N + +++G+RY+GE+ + + +EA + ++ + ++ LSGA+ DF+ +L+ +DF+
Sbjct: 184 NNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLE 243
Query: 260 KRLAKVRVDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPEYYKDDIIKGQFLTL 319
KRL + DAF + L++E R K TMID +L LQ++ PEYY D IIKG L +
Sbjct: 244 KRLKDISGKTDAFLRGLIEEHRTKKE--RANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 303
Query: 320 LAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINE 379
L AGTD++A TLEW+MS LLNHP +KK E++ VG RLV E+D KL YL+ +INE
Sbjct: 304 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 363
Query: 380 TYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERF-K 439
T RL++ P+L+P +S++C IGG+ +P+ T++++NAWA+HRDP +W FKPERF K
Sbjct: 364 TLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEK 423
Query: 440 QGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGL 499
+G E+ KL+ FGMGRRACPG GLA R +S+ LA LIQCF+W KL + ++DL G
Sbjct: 424 KG---ELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDW-KLINGDKIDLAERDGF 483
Query: 500 TMPKAIPLEAMCKPRASVTHLLSQ 520
T+ K +PL+AMCK R + + Q
Sbjct: 484 TLTKLVPLKAMCKSRPVINKVFKQ 499
BLAST of PI0005071 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 455.3 bits (1170), Expect = 9.3e-127
Identity = 224/483 (46.38%), Postives = 319/483 (66.05%), Query Frame = 0
Query: 28 FLTTLFLISKFRTTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLSQKYGPVFSLTLGF 87
F+ T+ ++ K + LPP PP++PIIG+LH LK P H+ LSQ YG +FSL G
Sbjct: 12 FIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGS 71
Query: 88 RPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRRIATT 147
R +VVVSSPS ECFTKNDI+ A+RPR L+GK + Y+YT +G+ +YG HWR +RRI T
Sbjct: 72 RLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTI 131
Query: 148 ELLSTKRLNDLSQIREEEV-KLWVKRLYGMTTEVKNGARVGLRSKLTELSFNIALMLMAG 207
++LS RLN +R +E +L K L + +E +V LR +LTE++FN + +++G
Sbjct: 132 DVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMISG 191
Query: 208 RRYFGEEVE-GESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLAKVRVD 267
+RY+G++ + + EEAK+ +E + ++ L GA+ DFL +L+++D KR ++
Sbjct: 192 KRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAKR 251
Query: 268 GDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPEYYKDDIIKGQFLTLLAAGTDTTA 327
+AF + L++E R+ + TMID +L L E+ PEYY D +IKG +L AGTDT+A
Sbjct: 252 SNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSA 311
Query: 328 STLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINETYRLFSALP 387
T+EW MS LLNHP +KKA E++ +G +LV E D +KL YLQ II+ET RL P
Sbjct: 312 VTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAP 371
Query: 388 ILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERFKQGETVEVSKL 447
+L+P SSEDCTIG FN+PK T+++ N W +HRDP W FKPERF++ E EV+K+
Sbjct: 372 LLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEE--EVNKV 431
Query: 448 LPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGLTMPKAIPLEA 507
+ FG+GRRACPG LA R V + LIQCFEW++ + ++++D+ G G+TMP IPL A
Sbjct: 432 MAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWER-ESEEKLDMMEGKGITMPMKIPLRA 491
Query: 508 MCK 509
MCK
Sbjct: 492 MCK 491
BLAST of PI0005071 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 446.4 bits (1147), Expect = 4.3e-124
Identity = 236/494 (47.77%), Postives = 318/494 (64.37%), Query Frame = 0
Query: 24 LFPIFLTTLFLISKFRTTHHSK-LPPTPPSLPIIGHLHLLKEPFHQMLQDLSQKYGPVFS 83
+ +F + L F T+ K LPP P LPIIG+LH LK+P H LSQKYG +FS
Sbjct: 9 IISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFS 68
Query: 84 LTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAYGQHWRIMR 143
L G R +VVVSS + QECFTKNDI+ A+RP L+GK + Y+ T V + YG HWR +R
Sbjct: 69 LWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 128
Query: 144 RIATTELLSTKRLNDLSQIREEEVKLWVKRL----YGMTTEVKNGARVGLRSKLTELSFN 203
RI + E+LS+ RLN +IR +E+ +++L Y TEV+ LR +E++FN
Sbjct: 129 RILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVE------LRPMFSEMTFN 188
Query: 204 IALMLMAGRRYFGEEVE-GESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRK 263
+ +++G+RY+G + + + EEA+ + +K+V L GA+ DFL L+ DF G K
Sbjct: 189 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEK 248
Query: 264 RLAKVRVDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPEYYKDDIIKGQFLTLL 323
RL K+ DAF Q L+DE R G TMID +L Q++ PEYY D IIKG + +L
Sbjct: 249 RLKKISKRTDAFLQGLIDEHR--FGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVML 308
Query: 324 AAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINET 383
AGTDT++ T+EWAMS LLNHP +KKA E++ +G R V E D +KL YLQ+I+ ET
Sbjct: 309 LAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYET 368
Query: 384 YRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERF-KQ 443
RL +A P+LVP SSED ++GG+NIP+ T+L+VNAW +HRDPN+W P CFKPERF K+
Sbjct: 369 LRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKE 428
Query: 444 GETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGLT 503
G EV+KLL FG+GRRACPG L+ R L L LIQCFEW ++ ++K +D+ G+T
Sbjct: 429 G---EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEK-IDMVEAKGIT 488
Query: 504 MPKAIPLEAMCKPR 511
K L AMCK R
Sbjct: 489 AGKKTSLNAMCKVR 490
BLAST of PI0005071 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 438.0 bits (1125), Expect = 1.5e-121
Identity = 217/493 (44.02%), Postives = 328/493 (66.53%), Query Frame = 0
Query: 25 FPIFLTTLFLISK--FRTTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLSQKYGPVFS 84
+ +F ++F I + F++ LPP P SLPIIG+LH LK P ++ + L++KYG V S
Sbjct: 9 YSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVIS 68
Query: 85 LTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAYGQHWRIMR 144
L G R +VVVSS S QECFTKND++ A+RPR LSGK + Y+YT +G+T+YG+HWR +R
Sbjct: 69 LWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128
Query: 145 RIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTELSFNIALM 204
RI + ++LS R+N+ + IR +E + +K+L A V L + +++FN +
Sbjct: 129 RITSLDVLSNHRINNFAPIRRDETQRLIKKL--AEDSSTKFAEVELTFRFFDMTFNNIMR 188
Query: 205 LMAGRRYFGEEVE-GESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLAK 264
+++G+RY+G++ + E +EA + ++ + ++ LSGA+ DF+ +LK +DF+ KR+ +
Sbjct: 189 MISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKR 248
Query: 265 VRVDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPEYYKDDIIKGQFLTLLAAGT 324
+ D F L+ E+R K TMID +L +QE+ PEYY D IIKG L +L AGT
Sbjct: 249 IGEKNDVFLSGLLQEQRSKKE--RTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAGT 308
Query: 325 DTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINETYRLF 384
D++A TLEW MS +LN+P +KK E++ VG RLV E+D KL YL+ +I ET RL+
Sbjct: 309 DSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLY 368
Query: 385 SALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERF-KQGETV 444
+ P+L+P ++++C +GG+ +P+ T++++NAWA+HRDP W FKPERF K+G
Sbjct: 369 TPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKG--- 428
Query: 445 EVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGLTMPKA 504
E+ K++ FGMGRRACPG GLA R +S+ LA L+QCF+W +++D+K +D+ G TM K
Sbjct: 429 ELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEK-IDMSERDGFTMTKL 488
Query: 505 IPLEAMCKPRASV 514
+PL+AMCK R V
Sbjct: 489 LPLKAMCKTRPVV 493
BLAST of PI0005071 vs. ExPASy TrEMBL
Match:
A0A0A0L2I1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1)
HSP 1 Score: 946.4 bits (2445), Expect = 4.9e-272
Identity = 481/525 (91.62%), Postives = 499/525 (95.05%), Query Frame = 0
Query: 1 MEQQTSFFFFFFSHFHPISIPLYLFPIFLTTLFLISKFRTTHHSK-LPPTPPSLPIIGHL 60
MEQQTSFFF HFHPISIP L IFLT+LFLISKFR T H+K LPP+PPSLP+IGHL
Sbjct: 1 MEQQTSFFFSQDHHFHPISIP--LLAIFLTSLFLISKFRRTQHTKLLPPSPPSLPVIGHL 60
Query: 61 HLLKEPFHQMLQDLSQKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSG 120
HLLKEPFHQMLQD S+KYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDI+FASRPRMLSG
Sbjct: 61 HLLKEPFHQMLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSG 120
Query: 121 KILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTT- 180
KILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLS+IREEEVKLWVKRLYG T
Sbjct: 121 KILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTR 180
Query: 181 -EVKNGARVGLRSKLTELSFNIALMLMAGRRYFGEEVE-GESEEAKELKETMKQVPLLSG 240
EV G RVGLRSKLTELSFNIALMLMAGRRYFG+E+E GESEEAKELKETMKQVPLLSG
Sbjct: 181 AEVNKGGRVGLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSG 240
Query: 241 ASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGS-CERKTMIDSMLL 300
ASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGS CERKTMIDSML
Sbjct: 241 ASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWCERKTMIDSMLC 300
Query: 301 LQEADPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGD 360
LQE+DPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPT ++KAWTEINQVVGD
Sbjct: 301 LQESDPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGD 360
Query: 361 QRLVQEADTNKLHYLQAIINETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWA 420
QRL++EADTNKLHYLQAIINETYRLF ALPILVPRESSEDCTIGGF+IPKGTMLVVNAWA
Sbjct: 361 QRLIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWA 420
Query: 421 VHRDPNVWEHPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQC 480
VHRDPNVWE PNCFKPERFKQGETVEV+KLLPFGMGRRACPGAGLAHRVVSLALATLIQC
Sbjct: 421 VHRDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQC 480
Query: 481 FEWDKLDDQKEVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 521
FEW KLD+QK+VDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL
Sbjct: 481 FEWGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523
BLAST of PI0005071 vs. ExPASy TrEMBL
Match:
A0A5D3D9B5 (Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002940 PE=3 SV=1)
HSP 1 Score: 916.8 bits (2368), Expect = 4.2e-263
Identity = 470/526 (89.35%), Postives = 490/526 (93.16%), Query Frame = 0
Query: 1 MEQQTSFFFFFFSHFHPISIPLYLFPIFLTTLFLISKFRTTHHSK--LPPTPPSLPIIGH 60
MEQQTS FFFFFSHF+ L IFLTTLFLISKFR + H+K LPP+PPSLP+IGH
Sbjct: 3 MEQQTS-FFFFFSHFN-----FPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGH 62
Query: 61 LHLLKEPFHQMLQDLSQKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLS 120
LHLLKEPFHQMLQDLS KYGPVFSLTLGFRPLVVVSSPSAV ECFTKNDI+FASRPRMLS
Sbjct: 63 LHLLKEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLS 122
Query: 121 GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTT 180
GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLND S++REEEVKLWVKRLYG T
Sbjct: 123 GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVT 182
Query: 181 --EVKNGARVGLRSKLTELSFNIALMLMAGRRYFGEEV-EGESEEAKELKETMKQVPLLS 240
EV+ G RVGLRSKLTELSFNIALML AGRRYFGEEV EGESEEAKELKETMKQVPLLS
Sbjct: 183 IREVQKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLS 242
Query: 241 GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGS-CERKTMIDSML 300
GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERR+LKGS CERKTMID+ML
Sbjct: 243 GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAML 302
Query: 301 LLQEADPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVG 360
LQE+DPEYYKDDIIKGQFLTLLAAGTDT ASTLEWAMSLLLNHP + KAWTEINQVVG
Sbjct: 303 SLQESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVG 362
Query: 361 DQRLVQEADTNKLHYLQAIINETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAW 420
DQRLV+EADTN LHYLQ+IINETYRLF ALPILVPRESSEDCTIGGF+IPKGTMLVVNAW
Sbjct: 363 DQRLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAW 422
Query: 421 AVHRDPNVWEHPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ 480
AVHR+P VWE PNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ
Sbjct: 423 AVHRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ 482
Query: 481 CFEWDKLDDQKEVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 521
CFEW KLD++K+VDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL
Sbjct: 483 CFEWGKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522
BLAST of PI0005071 vs. ExPASy TrEMBL
Match:
A0A1S3BTN1 (isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1)
HSP 1 Score: 916.8 bits (2368), Expect = 4.2e-263
Identity = 470/526 (89.35%), Postives = 490/526 (93.16%), Query Frame = 0
Query: 1 MEQQTSFFFFFFSHFHPISIPLYLFPIFLTTLFLISKFRTTHHSK--LPPTPPSLPIIGH 60
MEQQTS FFFFFSHF+ L IFLTTLFLISKFR + H+K LPP+PPSLP+IGH
Sbjct: 3 MEQQTS-FFFFFSHFN-----FPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGH 62
Query: 61 LHLLKEPFHQMLQDLSQKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLS 120
LHLLKEPFHQMLQDLS KYGPVFSLTLGFRPLVVVSSPSAV ECFTKNDI+FASRPRMLS
Sbjct: 63 LHLLKEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLS 122
Query: 121 GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTT 180
GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLND S++REEEVKLWVKRLYG T
Sbjct: 123 GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVT 182
Query: 181 --EVKNGARVGLRSKLTELSFNIALMLMAGRRYFGEEV-EGESEEAKELKETMKQVPLLS 240
EV+ G RVGLRSKLTELSFNIALML AGRRYFGEEV EGESEEAKELKETMKQVPLLS
Sbjct: 183 IREVQKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLS 242
Query: 241 GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGS-CERKTMIDSML 300
GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERR+LKGS CERKTMID+ML
Sbjct: 243 GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAML 302
Query: 301 LLQEADPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVG 360
LQE+DPEYYKDDIIKGQFLTLLAAGTDT ASTLEWAMSLLLNHP + KAWTEINQVVG
Sbjct: 303 SLQESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVG 362
Query: 361 DQRLVQEADTNKLHYLQAIINETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAW 420
DQRLV+EADTN LHYLQ+IINETYRLF ALPILVPRESSEDCTIGGF+IPKGTMLVVNAW
Sbjct: 363 DQRLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAW 422
Query: 421 AVHRDPNVWEHPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ 480
AVHR+P VWE PNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ
Sbjct: 423 AVHRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ 482
Query: 481 CFEWDKLDDQKEVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 521
CFEW KLD++K+VDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL
Sbjct: 483 CFEWGKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522
BLAST of PI0005071 vs. ExPASy TrEMBL
Match:
A0A6J1FJ78 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=1)
HSP 1 Score: 803.1 bits (2073), Expect = 6.7e-229
Identity = 399/507 (78.70%), Postives = 444/507 (87.57%), Query Frame = 0
Query: 15 FHPISIPLYLFPIFLTTLFLISKFRTTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLS 74
F +PL + + L LI KFR H LPP PPSLP+IGHLHLLKEPFHQMLQDLS
Sbjct: 11 FSNFEVPLL---VLILALLLIFKFRKAEHKNLPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70
Query: 75 QKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAY 134
QKYGP+ SL+LGFRP+VVVSSP+A QECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71 QKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130
Query: 135 GQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTE 194
GQHWRIMRRIATTELLST RLN L IR++EVKLW+K LY ++ KN RV +RSKLTE
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISG--KNNVRVEIRSKLTE 190
Query: 195 LSFNIALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQG 254
LSFNIALMLM G+RYFG+E +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D+QG
Sbjct: 191 LSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLVDYQG 250
Query: 255 FRKRLAKVRVDGDAFAQSLVDERRKLKG-SCERKTMIDSMLLLQEADPEYYKDDIIKGQF 314
FRKRLAKVRVDGDAFAQSL+DERR K ERKTMIDS+L +Q+ +PEYYKDDIIKGQF
Sbjct: 251 FRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIKGQF 310
Query: 315 LTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAI 374
LTLLAAGTDTTA TLEWA+SLLLNHPT++KKAWTEIN+ VGD+RLV+E D N+LHYLQ I
Sbjct: 311 LTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYLQGI 370
Query: 375 INETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPER 434
INETYRLF ALPILVPRESSEDCTIGGF++PKGTMLVVNAWA+HRDP VWE PN F+PER
Sbjct: 371 INETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFRPER 430
Query: 435 FKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGI 494
FK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW++LD+ KEVDL PGI
Sbjct: 431 FKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLTPGI 490
Query: 495 GLTMPKAIPLEAMCKPRASVTHLLSQL 521
GLTMPKA PL AM KPRA + HLLSQL
Sbjct: 491 GLTMPKARPLVAMFKPRAPIIHLLSQL 511
BLAST of PI0005071 vs. ExPASy TrEMBL
Match:
A0A6J1J4B3 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 SV=1)
HSP 1 Score: 798.9 bits (2062), Expect = 1.3e-227
Identity = 395/498 (79.32%), Postives = 440/498 (88.35%), Query Frame = 0
Query: 24 LFPIFLTTLFLISKFRTTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLSQKYGPVFSL 83
L I + L +I KFR H LPP PPSLP+IGHLHLLKEPFHQMLQDLSQKYGP+ SL
Sbjct: 18 LLLILVLALLMIFKFRKADHKNLPPGPPSLPVIGHLHLLKEPFHQMLQDLSQKYGPILSL 77
Query: 84 TLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRR 143
+LGFRP+VVVSSP+A QECF+KNDI+FASRPRM+SGKILNYDYTAVGATAYGQHWRIMRR
Sbjct: 78 SLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAYGQHWRIMRR 137
Query: 144 IATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTELSFNIALML 203
IATTELLST RLN L IR++EVKLW+K LY +T KN RV +RSKLTELSFNIALML
Sbjct: 138 IATTELLSTHRLNALINIRQQEVKLWLKSLYRITG--KNNVRVEIRSKLTELSFNIALML 197
Query: 204 MAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLAKVR 263
M G+RYFG+E +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D+QGFRK+LAKVR
Sbjct: 198 MVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLVDYQGFRKKLAKVR 257
Query: 264 VDGDAFAQSLVDERRKLKGS-CERKTMIDSMLLLQEADPEYYKDDIIKGQFLTLLAAGTD 323
VDGDAFAQSL+DERR K ERKTMIDS+L +Q+ +PEYYKD IIKGQFLTLLAAGTD
Sbjct: 258 VDGDAFAQSLIDERRNKKADHNERKTMIDSLLSMQQMEPEYYKDHIIKGQFLTLLAAGTD 317
Query: 324 TTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINETYRLFS 383
TTA TLEWA+SLLLNHPT++KKAWTEIN+ VGD+RLV+E D N+LHYLQ IINETYRLF
Sbjct: 318 TTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYLQGIINETYRLFP 377
Query: 384 ALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERFKQGETVEV 443
ALPILVPRESSEDCTIGGF++P GTMLVVNAWA+HRDP VWE PN F+PERFK+GE+VEV
Sbjct: 378 ALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPKVWEDPNSFRPERFKEGESVEV 437
Query: 444 SKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGLTMPKAIP 503
SKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW++LD+ KEVDL PGIGLTMPK+ P
Sbjct: 438 SKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLTPGIGLTMPKSSP 497
Query: 504 LEAMCKPRASVTHLLSQL 521
L AM KPRA +THLLSQL
Sbjct: 498 LVAMFKPRAPITHLLSQL 512
BLAST of PI0005071 vs. NCBI nr
Match:
XP_004141394.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012467 [Cucumis sativus])
HSP 1 Score: 946.4 bits (2445), Expect = 1.0e-271
Identity = 481/525 (91.62%), Postives = 499/525 (95.05%), Query Frame = 0
Query: 1 MEQQTSFFFFFFSHFHPISIPLYLFPIFLTTLFLISKFRTTHHSK-LPPTPPSLPIIGHL 60
MEQQTSFFF HFHPISIP L IFLT+LFLISKFR T H+K LPP+PPSLP+IGHL
Sbjct: 1 MEQQTSFFFSQDHHFHPISIP--LLAIFLTSLFLISKFRRTQHTKLLPPSPPSLPVIGHL 60
Query: 61 HLLKEPFHQMLQDLSQKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSG 120
HLLKEPFHQMLQD S+KYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDI+FASRPRMLSG
Sbjct: 61 HLLKEPFHQMLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSG 120
Query: 121 KILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTT- 180
KILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLS+IREEEVKLWVKRLYG T
Sbjct: 121 KILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTR 180
Query: 181 -EVKNGARVGLRSKLTELSFNIALMLMAGRRYFGEEVE-GESEEAKELKETMKQVPLLSG 240
EV G RVGLRSKLTELSFNIALMLMAGRRYFG+E+E GESEEAKELKETMKQVPLLSG
Sbjct: 181 AEVNKGGRVGLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSG 240
Query: 241 ASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGS-CERKTMIDSMLL 300
ASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGS CERKTMIDSML
Sbjct: 241 ASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWCERKTMIDSMLC 300
Query: 301 LQEADPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGD 360
LQE+DPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPT ++KAWTEINQVVGD
Sbjct: 301 LQESDPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGD 360
Query: 361 QRLVQEADTNKLHYLQAIINETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWA 420
QRL++EADTNKLHYLQAIINETYRLF ALPILVPRESSEDCTIGGF+IPKGTMLVVNAWA
Sbjct: 361 QRLIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWA 420
Query: 421 VHRDPNVWEHPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQC 480
VHRDPNVWE PNCFKPERFKQGETVEV+KLLPFGMGRRACPGAGLAHRVVSLALATLIQC
Sbjct: 421 VHRDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQC 480
Query: 481 FEWDKLDDQKEVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 521
FEW KLD+QK+VDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL
Sbjct: 481 FEWGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523
BLAST of PI0005071 vs. NCBI nr
Match:
XP_008452612.1 (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo] >KAA0064425.1 isoflavone 2'-hydroxylase-like [Cucumis melo var. makuwa] >TYK20162.1 isoflavone 2'-hydroxylase-like [Cucumis melo var. makuwa])
HSP 1 Score: 916.8 bits (2368), Expect = 8.6e-263
Identity = 470/526 (89.35%), Postives = 490/526 (93.16%), Query Frame = 0
Query: 1 MEQQTSFFFFFFSHFHPISIPLYLFPIFLTTLFLISKFRTTHHSK--LPPTPPSLPIIGH 60
MEQQTS FFFFFSHF+ L IFLTTLFLISKFR + H+K LPP+PPSLP+IGH
Sbjct: 3 MEQQTS-FFFFFSHFN-----FPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGH 62
Query: 61 LHLLKEPFHQMLQDLSQKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLS 120
LHLLKEPFHQMLQDLS KYGPVFSLTLGFRPLVVVSSPSAV ECFTKNDI+FASRPRMLS
Sbjct: 63 LHLLKEPFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLS 122
Query: 121 GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTT 180
GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLND S++REEEVKLWVKRLYG T
Sbjct: 123 GKILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVT 182
Query: 181 --EVKNGARVGLRSKLTELSFNIALMLMAGRRYFGEEV-EGESEEAKELKETMKQVPLLS 240
EV+ G RVGLRSKLTELSFNIALML AGRRYFGEEV EGESEEAKELKETMKQVPLLS
Sbjct: 183 IREVQKGGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLS 242
Query: 241 GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGS-CERKTMIDSML 300
GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERR+LKGS CERKTMID+ML
Sbjct: 243 GASYPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAML 302
Query: 301 LLQEADPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVG 360
LQE+DPEYYKDDIIKGQFLTLLAAGTDT ASTLEWAMSLLLNHP + KAWTEINQVVG
Sbjct: 303 SLQESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVG 362
Query: 361 DQRLVQEADTNKLHYLQAIINETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAW 420
DQRLV+EADTN LHYLQ+IINETYRLF ALPILVPRESSEDCTIGGF+IPKGTMLVVNAW
Sbjct: 363 DQRLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAW 422
Query: 421 AVHRDPNVWEHPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ 480
AVHR+P VWE PNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ
Sbjct: 423 AVHRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQ 482
Query: 481 CFEWDKLDDQKEVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 521
CFEW KLD++K+VDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL
Sbjct: 483 CFEWGKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522
BLAST of PI0005071 vs. NCBI nr
Match:
XP_038900261.1 (LOW QUALITY PROTEIN: cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 821.6 bits (2121), Expect = 3.8e-234
Identity = 424/522 (81.23%), Postives = 453/522 (86.78%), Query Frame = 0
Query: 1 MEQQTSFFFFFFSHFHPISIPLYLFPIFLTTLFLISKFRTTHHSKLPPTPPSLPIIGHLH 60
ME+Q S FFFSHF I LF + +TTLFL+SKFR T H KLPP+PPSLP+IGHLH
Sbjct: 1 MEEQAS---FFFSHFPTIP----LFALLITTLFLLSKFRKTEHRKLPPSPPSLPVIGHLH 60
Query: 61 LLKEPFHQMLQDLSQKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGK 120
LLKEPFHQMLQDLS+KYGPVFSLTLGFRP+VVVS+PSAVQE K
Sbjct: 61 LLKEPFHQMLQDLSKKYGPVFSLTLGFRPVVVVSAPSAVQE-----------------WK 120
Query: 121 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTT-- 180
ILNYDYTAVGATAYGQHWRIMRRIATTELLST RLN LS+IREEEVKLWVKRLYGMT
Sbjct: 121 ILNYDYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKRLYGMTEVG 180
Query: 181 EVKNGARVGLRSKLTELSFNIALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGAS 240
E G RVGLRSKLTELSFNIALMLM GRRYFG EVEGESEEAKELKETMKQVPLLSGAS
Sbjct: 181 ESNKGVRVGLRSKLTELSFNIALMLMTGRRYFGGEVEGESEEAKELKETMKQVPLLSGAS 240
Query: 241 YPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQE 300
YPADFLSVLK +DFQ VRVDGDAFAQS+VDERRKLKGSCERKT+IDS+LLLQE
Sbjct: 241 YPADFLSVLKWVDFQ--------VRVDGDAFAQSVVDERRKLKGSCERKTIIDSLLLLQE 300
Query: 301 ADPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRL 360
+DPEYYKD+IIKGQFLTLLAAGTDTTA TLEWAMSLLLNHPT +KKAWTEIN+VVGDQRL
Sbjct: 301 SDPEYYKDEIIKGQFLTLLAAGTDTTARTLEWAMSLLLNHPTEMKKAWTEINEVVGDQRL 360
Query: 361 VQEADTNKLHYLQAIINETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHR 420
V+EAD NK HYLQAIINETYRLF ALPIL PRESSEDCTIGGF+IPKGTMLVVNAWAVHR
Sbjct: 361 VEEADRNKPHYLQAIINETYRLFPALPILAPRESSEDCTIGGFDIPKGTMLVVNAWAVHR 420
Query: 421 DPNVWEHPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEW 480
DPNVWEHPN F+PERFK G+ +EVSKLLPFGMGRRA PGAGLAHRVV+LALATLIQCFEW
Sbjct: 421 DPNVWEHPNSFQPERFKPGQXLEVSKLLPFGMGRRAGPGAGLAHRVVALALATLIQCFEW 480
Query: 481 DKLDDQKEVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 521
+ DQK +DL PGIGLTMP+AIPLEA+CKPRAS+THLLSQL
Sbjct: 481 GR-PDQKGLDLSPGIGLTMPRAIPLEAICKPRASITHLLSQL 489
BLAST of PI0005071 vs. NCBI nr
Match:
XP_022940149.1 (cytochrome P450 81E8-like [Cucurbita moschata])
HSP 1 Score: 803.1 bits (2073), Expect = 1.4e-228
Identity = 399/507 (78.70%), Postives = 444/507 (87.57%), Query Frame = 0
Query: 15 FHPISIPLYLFPIFLTTLFLISKFRTTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLS 74
F +PL + + L LI KFR H LPP PPSLP+IGHLHLLKEPFHQMLQDLS
Sbjct: 11 FSNFEVPLL---VLILALLLIFKFRKAEHKNLPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70
Query: 75 QKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAY 134
QKYGP+ SL+LGFRP+VVVSSP+A QECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71 QKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130
Query: 135 GQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTE 194
GQHWRIMRRIATTELLST RLN L IR++EVKLW+K LY ++ KN RV +RSKLTE
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISG--KNNVRVEIRSKLTE 190
Query: 195 LSFNIALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQG 254
LSFNIALMLM G+RYFG+E +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D+QG
Sbjct: 191 LSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLVDYQG 250
Query: 255 FRKRLAKVRVDGDAFAQSLVDERRKLKG-SCERKTMIDSMLLLQEADPEYYKDDIIKGQF 314
FRKRLAKVRVDGDAFAQSL+DERR K ERKTMIDS+L +Q+ +PEYYKDDIIKGQF
Sbjct: 251 FRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIKGQF 310
Query: 315 LTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAI 374
LTLLAAGTDTTA TLEWA+SLLLNHPT++KKAWTEIN+ VGD+RLV+E D N+LHYLQ I
Sbjct: 311 LTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYLQGI 370
Query: 375 INETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPER 434
INETYRLF ALPILVPRESSEDCTIGGF++PKGTMLVVNAWA+HRDP VWE PN F+PER
Sbjct: 371 INETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFRPER 430
Query: 435 FKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGI 494
FK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW++LD+ KEVDL PGI
Sbjct: 431 FKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN-KEVDLTPGI 490
Query: 495 GLTMPKAIPLEAMCKPRASVTHLLSQL 521
GLTMPKA PL AM KPRA + HLLSQL
Sbjct: 491 GLTMPKARPLVAMFKPRAPIIHLLSQL 511
BLAST of PI0005071 vs. NCBI nr
Match:
XP_023523499.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 799.3 bits (2063), Expect = 2.0e-227
Identity = 397/507 (78.30%), Postives = 441/507 (86.98%), Query Frame = 0
Query: 15 FHPISIPLYLFPIFLTTLFLISKFRTTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLS 74
F +PL + + L LI KFR H LPP PPSLP+IGHLHLLKEPFHQMLQDLS
Sbjct: 11 FSNFEVPLL---VLILALLLILKFRKADHKNLPPGPPSLPVIGHLHLLKEPFHQMLQDLS 70
Query: 75 QKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAY 134
KYGP+ SL+LGFRP+VVVSSP+A QECF+KNDI+FASRPRM+SGKILNYDYTAVGATAY
Sbjct: 71 HKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGATAY 130
Query: 135 GQHWRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTE 194
GQHWRIMRRIATTELLST RLN L IR++EVKLW+K LY ++ KN RV +RSKLTE
Sbjct: 131 GQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISG--KNNVRVEIRSKLTE 190
Query: 195 LSFNIALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQG 254
LSFNIALMLM G+RYFG+E +SEEA+ELK TMKQVPLLSGASYPADFLSVLKL+D+QG
Sbjct: 191 LSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLVDYQG 250
Query: 255 FRKRLAKVRVDGDAFAQSLVDERRKLKG-SCERKTMIDSMLLLQEADPEYYKDDIIKGQF 314
FRKRLAKVRVDGDAFAQSL+DERR K ERKTMIDS+L +Q+ +PEYYKDDIIKGQF
Sbjct: 251 FRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDIIKGQF 310
Query: 315 LTLLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAI 374
LTLLAAGTDTTA TLEWA+SLLLNHPT++KKAWTEIN+ VGD+RLV+E D N+LHYLQ I
Sbjct: 311 LTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLHYLQGI 370
Query: 375 INETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPER 434
INETYRLF ALPILVPRESSEDCTIGGF++PKGTMLVVNAWA+HRDP VWE PN F+PER
Sbjct: 371 INETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNSFRPER 430
Query: 435 FKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGI 494
FK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW++L D KEVDL PGI
Sbjct: 431 FKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERL-DSKEVDLTPGI 490
Query: 495 GLTMPKAIPLEAMCKPRASVTHLLSQL 521
GLTMPKA PL AM KPR + HLLSQL
Sbjct: 491 GLTMPKASPLVAMFKPRTPIIHLLSQL 511
BLAST of PI0005071 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 445.3 bits (1144), Expect = 6.8e-125
Identity = 232/497 (46.68%), Postives = 333/497 (67.00%), Query Frame = 0
Query: 27 IFLTTLFLISKFRTTHHSKLPPTPP-SLPIIGHLHLLKEPFHQMLQDLSQKY--GPVFSL 86
+ L+ ++LI K + LPP+P SLP+IGHL LLK P H+ LSQ P+FSL
Sbjct: 13 VVLSLIYLIGKLK--RKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSL 72
Query: 87 TLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRR 146
LG R + V SS S +ECFTKND++ A+RP + K + YDYT + A +YG HWR +RR
Sbjct: 73 RLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLRR 132
Query: 147 IATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTELSFNIALML 206
I + E+ S RLN IR++E++ V RL ++ +V ++S L++L+FN L +
Sbjct: 133 IGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQ--EFVKVDMKSMLSDLTFNNILRM 192
Query: 207 MAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKRLAKVR 266
+AG+RY+G+ VE + EAK +++ + V +GA D+L VL+L+ + R+ K+
Sbjct: 193 VAGKRYYGDGVE-DDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLA 252
Query: 267 VDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPEYYKDDIIKGQFLTLLAAGTDT 326
D F Q LVDE+R+ K + TMID +L LQE+ P+Y+ D IIKG L L+ AGTDT
Sbjct: 253 GRLDEFLQGLVDEKREAKE--KGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDT 312
Query: 327 TASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINETYRLFSA 386
+A TLEWA+S +LNHP + KA EI++ +G RL+ E+D + L YLQ I++ET RL+ A
Sbjct: 313 SAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPA 372
Query: 387 LPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERF-KQGETVEV 446
P+L+P +SEDC + G+++P+GT+L+ N WA+HRDP +W+ P FKPERF K+G E
Sbjct: 373 APMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEG---EA 432
Query: 447 SKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGLTMPKAIP 506
KL+PFG+GRRACPG+GLAHR+++L L +LIQC EW+K+ + EVD+ G G+TMPKA P
Sbjct: 433 QKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE--EVDMSEGKGVTMPKAKP 492
Query: 507 LEAMCKPRASVTHLLSQ 520
LEAMC+ R SV + ++
Sbjct: 493 LEAMCRARPSVVKIFNE 495
BLAST of PI0005071 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 444.9 bits (1143), Expect = 8.9e-125
Identity = 238/500 (47.60%), Postives = 328/500 (65.60%), Query Frame = 0
Query: 22 LYLFPIFLTTLFLISKFRTTHHSKLPPTPP-SLPIIGHLHLLKEPFHQMLQDLSQKYG-- 81
L +F +FL++ R+ LPP+P SLP+IGHLHLLK P H+ LS+ G
Sbjct: 9 LSFLSLFTLAIFLLT--RSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNA 68
Query: 82 PVFSLTLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAYGQHW 141
PVF L LG R + V+SS S +ECFTKND++ A+RP+ K L Y+ T + + +YG HW
Sbjct: 69 PVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHW 128
Query: 142 RIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTELSFN 201
R +RRIA E+ ST RLN IR++E++ + L + V +++ LT L+ N
Sbjct: 129 RNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHL--SRDSLHGFVEVEMKTLLTNLASN 188
Query: 202 IALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKR 261
+ ++AG+RYFGE ++++AK +K + + +GA P D+LS+L+ + + KR
Sbjct: 189 TTIRMLAGKRYFGE----DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKR 248
Query: 262 LAKVRVDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPEYYKDDIIKGQFLTLLA 321
+ + D F Q LVDE+R K E TMID +L LQ+ P+YY D IIKG LTL+
Sbjct: 249 IKNLGNRFDTFLQKLVDEKRAEKEKGE--TMIDHLLALQDIQPDYYTDVIIKGIILTLII 308
Query: 322 AGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINETY 381
AGTDT++ TLEWAMS LLNHP +KKA EI++ VG RLV E+D L YLQ+I+ ET
Sbjct: 309 AGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETL 368
Query: 382 RLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERF-KQG 441
R++ A+P+L+P SSEDC +GG++IP GTM++ NAWA+HRDP VWE P FKPERF K+G
Sbjct: 369 RMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEG 428
Query: 442 ETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGLTM 501
E KL+ FGMGRRACPGAGLAHR+++ AL +L+QCFEW+++ + VD+ G T+
Sbjct: 429 ---EAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGED-FVDMTEDKGATL 488
Query: 502 PKAIPLEAMCKPRASVTHLL 518
PKAIPL AMCK R+ V L+
Sbjct: 489 PKAIPLRAMCKARSIVDKLI 492
BLAST of PI0005071 vs. TAIR 10
Match:
AT5G67310.1 (cytochrome P450, family 81, subfamily G, polypeptide 1 )
HSP 1 Score: 442.6 bits (1137), Expect = 4.4e-124
Identity = 235/492 (47.76%), Postives = 316/492 (64.23%), Query Frame = 0
Query: 20 IPLYLFPIFLTTLFLISKFRTTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLSQKYG- 79
I L+L + + + FR+ LPP P P+IGHLHLLKEP H+ L+DLS+ G
Sbjct: 16 IDLFLLALMAGFIAVAYVFRSKQKKNLPPNPVGFPVIGHLHLLKEPVHRSLRDLSRNLGI 75
Query: 80 PVFSLTLGFRPLVVVSSPSAVQECFT-KNDILFASRPRMLSGKILNYDYTAVGATAYGQH 139
VF L LG R VVV+S SA +E + +ND++FA+RP + + Y+ T V YG+H
Sbjct: 76 DVFILRLGSRRAVVVTSASAAEEFLSQQNDVVFANRPLATLTEYMGYNNTLVSTAPYGEH 135
Query: 140 WRIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRLYGMTTEVKNGARVGLRSKLTELSF 199
WR +RR ++LST RL D S IR +EV+ ++++ R+ L+ L L++
Sbjct: 136 WRRLRRFCAVDILSTARLRDFSDIRRDEVRAMIRKINVELVTSGGSVRLKLQPFLYGLTY 195
Query: 200 NIALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRK 259
NI + ++AG+R E EE KE+++ +++V +G +Y DFL LKL D G+RK
Sbjct: 196 NILMSMVAGKR-------EEDEETKEVRKLIREVFDFAGVNYVGDFLPTLKLFDLDGYRK 255
Query: 260 RLAKVRVDGDAFAQSLVDERRKLKGSCE-RKTMIDSMLLLQEADPEYYKDDIIKGQFLTL 319
R K+ D F Q LVDE RK +G E KTMI +L LQE++PE Y DDIIKG +
Sbjct: 256 RAKKLASKLDKFMQKLVDEHRKNRGKAELEKTMITRLLSLQESEPECYTDDIIKGLVQVM 315
Query: 320 LAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINE 379
L AGTDTTA TLEWAM+ LLNHP ++K TE+N+V + R+ +E+DT K YL +I+E
Sbjct: 316 LLAGTDTTAVTLEWAMANLLNHPEVLRKLKTELNEVSKEGRVFEESDTGKCPYLNNVISE 375
Query: 380 TYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERFKQ 439
T RLF A P+LVP SS DC + GF+IP+ T L +NAWA+ RDPNVW+ P FKPERF +
Sbjct: 376 TLRLFPAAPLLVPHASSTDCEVAGFDIPRRTWLFINAWAIQRDPNVWDDPETFKPERF-E 435
Query: 440 GETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGLT 499
ET K LPFG+GRRACPG GLA V+SLAL +LIQCF+W++ D+ VD+ G GLT
Sbjct: 436 SET-HRGKFLPFGIGRRACPGMGLAQLVLSLALGSLIQCFDWER-DNDVAVDMSEGKGLT 495
Query: 500 MPKAIPLEAMCK 509
MPK++PL A CK
Sbjct: 496 MPKSVPLVAKCK 497
BLAST of PI0005071 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 433.7 bits (1114), Expect = 2.1e-121
Identity = 232/500 (46.40%), Postives = 331/500 (66.20%), Query Frame = 0
Query: 27 IFLTTLFLISKFRTTHHSKLPPTPP-SLPIIGHLHLLKEPFHQMLQDLSQKYG--PVFSL 86
I L+ +FLI + + LPP+P +LP+IGHL LLK P H++ +SQ G P+ SL
Sbjct: 13 IVLSLIFLIGRIK--RKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISL 72
Query: 87 TLGFRPLVVVSSPSAVQECFTKNDILFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRR 146
LG R L VVSS S +ECFTKND++ A+R +S K ++Y + V + +Y +HWR +RR
Sbjct: 73 RLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRR 132
Query: 147 IATTELLSTKRLNDLSQIREEEVKLWVKRL-----YGMTTEVKNGARVGLRSKLTELSFN 206
I E+ S RLN S IR +E++ + RL YG T +V ++S ++L+FN
Sbjct: 133 IGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFT-------KVEMKSMFSDLTFN 192
Query: 207 IALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGFRKR 266
+ ++AG+ Y+G+ E + EAK ++ + + SG AD++ +L I + R
Sbjct: 193 NIIRMLAGKCYYGDGKE-DDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETR 252
Query: 267 LAKVRVDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPEYYKDDIIKGQFLTLLA 326
+ K+ D F Q LVDE+R+ K E TM+D +L LQE PEYY D IIKG L+L+A
Sbjct: 253 IKKLAGRLDEFLQGLVDEKREGKEKKE-NTMVDHLLCLQETQPEYYMDRIIKGTMLSLIA 312
Query: 327 AGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIINETY 386
GTDTTA TLEWA+S LLN+P + KA EI++++G RL++E+D L YLQ I++ET
Sbjct: 313 GGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETL 372
Query: 387 RLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERF-KQG 446
RL+ A P+L+P +S+DC +GG+++P+GTML+ NAWA+HRDP +W+ P FKPERF K+G
Sbjct: 373 RLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEG 432
Query: 447 ETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGIGLTM 506
E KL+PFG+GRRACPG+GLA R+V+L+L +LIQCFEW+++ ++ EVD+ G GLTM
Sbjct: 433 ---EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEE-EVDMTEGPGLTM 492
Query: 507 PKAIPLEAMCKPRASVTHLL 518
PKA PLEAMC+ R V +L
Sbjct: 493 PKARPLEAMCRARDFVGKIL 495
BLAST of PI0005071 vs. TAIR 10
Match:
AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2 )
HSP 1 Score: 430.6 bits (1106), Expect = 1.7e-120
Identity = 221/504 (43.85%), Postives = 327/504 (64.88%), Query Frame = 0
Query: 24 LFPIFLTTLFLISKFR----TTHHSKLPPTPPSLPIIGHLHLLKEPFHQMLQDLSQKYGP 83
L+ I L LFL+ ++ T LPP PPS P +GHLHL+K P H++LQ S +YGP
Sbjct: 2 LYFILLPLLFLVISYKFLYSKTQRFNLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGP 61
Query: 84 VFSLTLGFRPLVVVSSPSAVQECFT-KNDILFASRPRMLSGKILNYDYTAVGATAYGQHW 143
+FSL G R +VV++SPS QE FT +NDI+ +SRP L+ K + Y++T VG YG HW
Sbjct: 62 IFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHW 121
Query: 144 RIMRRIATTELLSTKRLNDLSQIREEEVKLWVKRL--YGMTTEVKNG-ARVGLRSKLTEL 203
R +RRI + E+LS+ RL + IR++E+ + RL Y T+ N + L L++L
Sbjct: 122 RNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDL 181
Query: 204 SFNIALMLMAGRRYFGEEVEGESEEAKELKETMKQVPLLSGASYPADFLSVLKLIDFQGF 263
+FN + ++ G+RY+G++V + EEA+ K+ + + + SGA++ AD+L +LKL F
Sbjct: 182 TFNNIVRMVTGKRYYGDDVNNK-EEAELFKKLVYDIAMYSGANHSADYLPILKLFG-NKF 241
Query: 264 RKRLAKVRVDGDAFAQSLVDERRKLKGSCERKTMIDSMLLLQEADPEYYKDDIIKGQFLT 323
K + + D Q L+DE R+ K E TM++ ++ LQ+ PEYY D IIKG ++
Sbjct: 242 EKEVKAIGKSMDDILQRLLDECRRDK---EGNTMVNHLISLQQQQPEYYTDVIIKGLMMS 301
Query: 324 LLAAGTDTTASTLEWAMSLLLNHPTSIKKAWTEINQVVGDQRLVQEADTNKLHYLQAIIN 383
++ AGT+T+A TLEWAM+ LL +P ++KA +EI++ +G RL+ E+D L YLQ +++
Sbjct: 302 MMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVS 361
Query: 384 ETYRLFSALPILVPRESSEDCTIGGFNIPKGTMLVVNAWAVHRDPNVWEHPNCFKPERFK 443
ET+RLF P L+PR ++D IGG+++P+ T+++VNAWA+HRDP +WE P F P+R+
Sbjct: 362 ETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYN 421
Query: 444 Q--GETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWDKLDDQKEVDLCPGI 503
G V KL+PFG GRR CPGAGL R+V+LAL +LIQCFEW+ + + E+D+
Sbjct: 422 DGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGE-EMDMSEST 481
Query: 504 GLTMPKAIPLEAMCKPRASVTHLL 518
GL M K PL AMC+PR ++ LL
Sbjct: 482 GLGMRKMDPLRAMCRPRPIMSKLL 499
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 3.1e-138 | 49.90 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
P93147 | 1.1e-127 | 46.23 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNQ9 | 9.3e-127 | 46.38 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Q6WNQ8 | 4.3e-124 | 47.77 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q6WNR0 | 1.5e-121 | 44.02 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0L2I1 | 4.9e-272 | 91.62 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1 | [more] |
A0A5D3D9B5 | 4.2e-263 | 89.35 | Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
A0A1S3BTN1 | 4.2e-263 | 89.35 | isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1 | [more] |
A0A6J1FJ78 | 6.7e-229 | 78.70 | cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=... | [more] |
A0A6J1J4B3 | 1.3e-227 | 79.32 | isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 ... | [more] |
Match Name | E-value | Identity | Description | |
XP_004141394.1 | 1.0e-271 | 91.62 | cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012... | [more] |
XP_008452612.1 | 8.6e-263 | 89.35 | PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo] >KAA0064425.1 isoflavon... | [more] |
XP_038900261.1 | 3.8e-234 | 81.23 | LOW QUALITY PROTEIN: cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
XP_022940149.1 | 1.4e-228 | 78.70 | cytochrome P450 81E8-like [Cucurbita moschata] | [more] |
XP_023523499.1 | 2.0e-227 | 78.30 | cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
AT4G37370.1 | 6.8e-125 | 46.68 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37330.1 | 8.9e-125 | 47.60 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT5G67310.1 | 4.4e-124 | 47.76 | cytochrome P450, family 81, subfamily G, polypeptide 1 | [more] |
AT4G37360.1 | 2.1e-121 | 46.40 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37430.1 | 1.7e-120 | 43.85 | cytochrome P450, family 91, subfamily A, polypeptide 2 | [more] |