PI0005063 (gene) Melon (PI 482460) v1

Overview
NamePI0005063
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionervatamin-B-like
Locationchr07: 2675627 .. 2676820 (-)
RNA-Seq ExpressionPI0005063
SyntenyPI0005063
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTGGAATGTGGGTTTGATTTATCTGATTCTCTGGGTTTTCTGGACACCCTCACTGGTATCCATGGCAATGGACCACCCTCCAGGATCTAGTTCCGGTGACTTACAAGACAGGTACCAGAAATGGATGAATAAATACGGTCGACAATACAAGTGCAGAGAAGAATGGGAGCAGAGATTCACAATTTATCAGGCGAATGTTCAGTACATTGACAACTTCAATTCTCTGAATCAGTCATATACACTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGACAACTTACTTGGGGTATAAAACCGTTTCGGTTCCTGATACATTCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGTACGAACTCACAATACTCTCATTCATCCAACTAATCAGAAATACGTTGAACATGTTCTCTTTAAGTCTGATTATGAGTTTTTTTTCCCTAACAAGTACCCTAAACCCCACCCTCTATCCATTGTAAGTCATTCCATCATATTTGAAAAACTTACCAGTTTCCAATTTTCGACACAGGGAGCTGCTGGGCATTCTCTGCAGTTGCAGCAGTGGAAGGCATCAACAAAATAAAAGCAGGGAAGTTGATGTCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGGCCTCGGGGAACCAGGGATGCAGTGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAAAACTGGACTAACTACAGAAACAGAATATCCATATAAAGGAACTGAATCTGCATGCAACAAACAAAAAGCGAAATACCAGTCCGTGTCAATAACTGGATATGAAAAAGTACCCATCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAAACAGCCAGTCTCTGTAGCAATTGATGCAGGGGGGAATGATTTTCAGTTCTATTCTGGTGGAATCTTCTCGGGCAATTGTGGAAAGCAACTCAATCATGGAGTGACAATAGTTGGGTATGGGGAAGATACCAATAAAGCTTATTGGCTTGTCAAGAATTCATGGGGCAATGACTGGGGTGAATCGGGTTACATAAGAATGAAGCGTGATTCAACTGATAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCATCAAAGACTGA

mRNA sequence

ATGATTTGGAATGTGGGTTTGATTTATCTGATTCTCTGGGTTTTCTGGACACCCTCACTGGTATCCATGGCAATGGACCACCCTCCAGGATCTAGTTCCGGTGACTTACAAGACAGGTACCAGAAATGGATGAATAAATACGGTCGACAATACAAGTGCAGAGAAGAATGGGAGCAGAGATTCACAATTTATCAGGCGAATGTTCAGTACATTGACAACTTCAATTCTCTGAATCAGTCATATACACTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGACAACTTACTTGGGGTATAAAACCGTTTCGGTTCCTGATACATTCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGGAGCTGCTGGGCATTCTCTGCAGTTGCAGCAGTGGAAGGCATCAACAAAATAAAAGCAGGGAAGTTGATGTCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGGCCTCGGGGAACCAGGGATGCAGTGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAAAACTGGACTAACTACAGAAACAGAATATCCATATAAAGGAACTGAATCTGCATGCAACAAACAAAAAGCGAAATACCAGTCCGTGTCAATAACTGGATATGAAAAAGTACCCATCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAAACAGCCAGTCTCTGTAGCAATTGATGCAGGGGGGAATGATTTTCAGTTCTATTCTGGTGGAATCTTCTCGGGCAATTGTGGAAAGCAACTCAATCATGGAGTGACAATAGTTGGGTATGGGGAAGATACCAATAAAGCTTATTGGCTTGTCAAGAATTCATGGGGCAATGACTGGGGTGAATCGGGTTACATAAGAATGAAGCGTGATTCAACTGATAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCATCAAAGACTGA

Coding sequence (CDS)

ATGATTTGGAATGTGGGTTTGATTTATCTGATTCTCTGGGTTTTCTGGACACCCTCACTGGTATCCATGGCAATGGACCACCCTCCAGGATCTAGTTCCGGTGACTTACAAGACAGGTACCAGAAATGGATGAATAAATACGGTCGACAATACAAGTGCAGAGAAGAATGGGAGCAGAGATTCACAATTTATCAGGCGAATGTTCAGTACATTGACAACTTCAATTCTCTGAATCAGTCATATACACTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGACAACTTACTTGGGGTATAAAACCGTTTCGGTTCCTGATACATTCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGGAGCTGCTGGGCATTCTCTGCAGTTGCAGCAGTGGAAGGCATCAACAAAATAAAAGCAGGGAAGTTGATGTCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGGCCTCGGGGAACCAGGGATGCAGTGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAAAACTGGACTAACTACAGAAACAGAATATCCATATAAAGGAACTGAATCTGCATGCAACAAACAAAAAGCGAAATACCAGTCCGTGTCAATAACTGGATATGAAAAAGTACCCATCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAAACAGCCAGTCTCTGTAGCAATTGATGCAGGGGGGAATGATTTTCAGTTCTATTCTGGTGGAATCTTCTCGGGCAATTGTGGAAAGCAACTCAATCATGGAGTGACAATAGTTGGGTATGGGGAAGATACCAATAAAGCTTATTGGCTTGTCAAGAATTCATGGGGCAATGACTGGGGTGAATCGGGTTACATAAGAATGAAGCGTGATTCAACTGATAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCATCAAAGACTGA

Protein sequence

MIWNVGLIYLILWVFWTPSLVSMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWLVKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD
Homology
BLAST of PI0005063 vs. ExPASy Swiss-Prot
Match: Q9FJ47 (Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana OX=3702 GN=SAG12 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.0e-94
Identity = 174/313 (55.59%), Postives = 226/313 (72.20%), Query Frame = 0

Query: 36  LQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSL--NQSYTLAENNFADLTN 95
           +Q R+ +WM K+GR Y   +E   R+ +++ NV+ I++ NS+   +++ LA N FADLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 96  EEFKTTYLGYKTVSVPDTF-------FRYGNMVN--LPTNVDWRKEGAVTPIKNQGQCGS 155
           +EF++ Y G+K VS   +        FRY N+ +  LP +VDWRK+GAVTPIKNQG CG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGC 153

Query: 156 CWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIKKT-GLT 215
           CWAFSAVAA+EG  +IK GKL+SLSEQ+LVDCD  + + GC GG M  AFE IK T GLT
Sbjct: 154 CWAFSAVAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATGGLT 213

Query: 216 TETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAGGNDF 275
           TE+ YPYKG ++ CN +K   ++ SITGYE VP+NDE++L  AVA QPVSV I+ GG DF
Sbjct: 214 TESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDF 273

Query: 276 QFYSGGIFSGNCGKQLNHGVTIVGYGEDTN-KAYWLVKNSWGNDWGESGYIRMKRDSTDK 335
           QFYS G+F+G C   L+H VT +GYGE TN   YW++KNSWG  WGESGY+R+++D  DK
Sbjct: 274 QFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDK 333

BLAST of PI0005063 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.5e-93
Identity = 180/344 (52.33%), Postives = 232/344 (67.44%), Query Frame = 0

Query: 11  ILWVFWTPSLV-----SMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQ 70
           +LWV  + SLV     S         S   L D Y++W + +       E+  +RF +++
Sbjct: 6   LLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEK-HKRFNVFK 65

Query: 71  ANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTF---------FRYG 130
           ANV ++ N N +++ Y L  N FAD+TN EF++TY G K V+    F         F Y 
Sbjct: 66  ANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSK-VNHHKMFRGSQHGSGTFMYE 125

Query: 131 NMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDC 190
            + ++P +VDWRK+GAVT +K+QGQCGSCWAFS + AVEGIN+IK  KL+SLSEQELVDC
Sbjct: 126 KVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELVDC 185

Query: 191 DVASGNQGCSGGYMYKAFEFIK-KTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKV 250
           D    NQGC+GG M  AFEFIK K G+TTE+ YPY   E  C++ K    +VSI G+E V
Sbjct: 186 D-KEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGHENV 245

Query: 251 PINDEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKA 310
           P+NDE +L  AVA QPVSVAIDAGG+DFQFYS G+F+G+C   LNHGV IVGYG   +  
Sbjct: 246 PVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTTVDGT 305

Query: 311 -YWLVKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
            YW+V+NSWG +WGE GYIRM+R+ + K+G CGIAMMASYPIK+
Sbjct: 306 NYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIKN 346

BLAST of PI0005063 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-92
Identity = 180/344 (52.33%), Postives = 233/344 (67.73%), Query Frame = 0

Query: 11  ILWVFWTPSLV-----SMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQ 70
           +LWV  + SLV     S        +S   L D Y++W + +       E+  +RF +++
Sbjct: 6   LLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEK-HKRFNVFK 65

Query: 71  ANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTF---------FRYG 130
           AN+ ++ N N +++ Y L  N FAD+TN EF++TY G K V+ P  F         F Y 
Sbjct: 66  ANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSK-VNHPRMFRGTPHENGAFMYE 125

Query: 131 NMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDC 190
            +V++P +VDWRK+GAVT +K+QGQCGSCWAFS V AVEGIN+IK  KL++LSEQELVDC
Sbjct: 126 KVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDC 185

Query: 191 DVASGNQGCSGGYMYKAFEFIK-KTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKV 250
           D    NQGC+GG M  AFEFIK K G+TTE+ YPYK  E  C+  K    +VSI G+E V
Sbjct: 186 D-KEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENV 245

Query: 251 PINDEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKA 310
           P NDE +L  AVA QPVSVAIDAGG+DFQFYS G+F+G+C   LNHGV IVGYG   +  
Sbjct: 246 PANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTTVDGT 305

Query: 311 -YWLVKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
            YW+V+NSWG +WGE GYIRM+R+ + K+G CGIAM+ SYPIK+
Sbjct: 306 NYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIKN 346

BLAST of PI0005063 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.5e-90
Identity = 172/317 (54.26%), Postives = 215/317 (67.82%), Query Frame = 0

Query: 32  SSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSLNQSYTLAENNFADL 91
           S   L + Y++W + +    +  EE  +RF +++ NV++I   N  ++SY L  N F D+
Sbjct: 30  SENSLWELYERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDM 89

Query: 92  TNEEFKTTYLG--------YKTVSVPDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCG 151
           T+EEF+ TY G        ++        F Y N+  LPT+VDWRK GAVTP+KNQGQCG
Sbjct: 90  TSEEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCG 149

Query: 152 SCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIK-KTGL 211
           SCWAFS V AVEGIN+I+  KL SLSEQELVDCD  + NQGC+GG M  AFEFIK K GL
Sbjct: 150 SCWAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGL 209

Query: 212 TTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAGGND 271
           T+E  YPYK ++  C+  K     VSI G+E VP N E  L  AVA QPVSVAIDAGG+D
Sbjct: 210 TSELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSD 269

Query: 272 FQFYSGGIFSGNCGKQLNHGVTIVGYGEDTN-KAYWLVKNSWGNDWGESGYIRMKRDSTD 331
           FQFYS G+F+G CG +LNHGV +VGYG   +   YW+VKNSWG +WGE GYIRM+R    
Sbjct: 270 FQFYSEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRH 329

Query: 332 KQGTCGIAMMASYPIKD 339
           K+G CGIAM ASYP+K+
Sbjct: 330 KEGLCGIAMEASYPLKN 344

BLAST of PI0005063 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.5e-90
Identity = 170/317 (53.63%), Postives = 215/317 (67.82%), Query Frame = 0

Query: 32  SSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSLNQSYTLAENNFADL 91
           S   L   Y +W + +       E  E+RF +++ NV ++ N N  N+SY L  N FADL
Sbjct: 30  SEEGLSTLYDRWRSHHSVPRSLNER-EKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADL 89

Query: 92  TNEEFKTTYLG-----YKTVSVP-----DTFFRYGNMVNLPTNVDWRKEGAVTPIKNQGQ 151
           T  EFK  Y G     ++ +  P        + + N+  LP++VDWRK+GAVT IKNQG+
Sbjct: 90  TINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGK 149

Query: 152 CGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIKKT- 211
           CGSCWAFS VAAVEGINKIK  KL+SLSEQELVDCD    N+GC+GG M  AFEFIKK  
Sbjct: 150 CGSCWAFSTVAAVEGINKIKTNKLVSLSEQELVDCDTKQ-NEGCNGGLMEIAFEFIKKNG 209

Query: 212 GLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAGG 271
           G+TTE  YPY+G +  C+  K     V+I G+E VP NDE +L  AVA QPVSVAIDAG 
Sbjct: 210 GITTEDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGS 269

Query: 272 NDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWLVKNSWGNDWGESGYIRMKRDST 331
           +DFQFYS G+F+G+CG +LNHGV  VGYG +  K YW+V+NSWG +WGE GYI+++R+  
Sbjct: 270 SDFQFYSEGVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREID 329

Query: 332 DKQGTCGIAMMASYPIK 338
           + +G CGIAM ASYPIK
Sbjct: 330 EPEGRCGIAMEASYPIK 344

BLAST of PI0005063 vs. ExPASy TrEMBL
Match: A0A5A7SQK0 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001250 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 1.9e-176
Identity = 305/341 (89.44%), Postives = 319/341 (93.55%), Query Frame = 0

Query: 1   MIWNVGLIYLILWVFWTPSLVSMAMDHPPGSS-SGDLQDRYQKWMNKYGRQYKCREEWEQ 60
           MIW+V LI LILWVFWTP+ VSMAMD+P GSS SG+LQDRYQKWM+KYGRQYK REEWEQ
Sbjct: 6   MIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWEQ 65

Query: 61  RFTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVP--DTFFRYGN 120
           RFTIYQANVQYIDNFNSLN SYTLAENNF DLTNEEF  TYLGY+TVS+P  DTFFRYGN
Sbjct: 66  RFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYGN 125

Query: 121 MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD 180
           MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD
Sbjct: 126 MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD 185

Query: 181 VASGNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPI 240
           V SGNQGC+GGYMYKAFEFIKKTGLTTE EYPY  T SAC+KQK KYQSVSI+GYEKVP+
Sbjct: 186 VTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVPV 245

Query: 241 NDEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYW 300
           NDEKSL+AAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGV IVGYGED+N+AYW
Sbjct: 246 NDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQAYW 305

Query: 301 LVKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
           LVKNSWG  WGESGYIRM RDSTDKQGTCGIAMMASYPIKD
Sbjct: 306 LVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

BLAST of PI0005063 vs. ExPASy TrEMBL
Match: A0A1S3C828 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 1.9e-176
Identity = 305/341 (89.44%), Postives = 319/341 (93.55%), Query Frame = 0

Query: 1   MIWNVGLIYLILWVFWTPSLVSMAMDHPPGSS-SGDLQDRYQKWMNKYGRQYKCREEWEQ 60
           MIW+V LI LILWVFWTP+ VSMAMD+P GSS SG+LQDRYQKWM+KYGRQYK REEWEQ
Sbjct: 6   MIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWEQ 65

Query: 61  RFTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVP--DTFFRYGN 120
           RFTIYQANVQYIDNFNSLN SYTLAENNF DLTNEEF  TYLGY+TVS+P  DTFFRYGN
Sbjct: 66  RFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYGN 125

Query: 121 MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD 180
           MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD
Sbjct: 126 MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD 185

Query: 181 VASGNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPI 240
           V SGNQGC+GGYMYKAFEFIKKTGLTTE EYPY  T SAC+KQK KYQSVSI+GYEKVP+
Sbjct: 186 VTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVPV 245

Query: 241 NDEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYW 300
           NDEKSL+AAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGV IVGYGED+N+AYW
Sbjct: 246 NDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQAYW 305

Query: 301 LVKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
           LVKNSWG  WGESGYIRM RDSTDKQGTCGIAMMASYPIKD
Sbjct: 306 LVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

BLAST of PI0005063 vs. ExPASy TrEMBL
Match: A0A0A0LJV6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292830 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.8e-172
Identity = 296/340 (87.06%), Postives = 316/340 (92.94%), Query Frame = 0

Query: 1   MIW-NVGLIYLILWVFWTPSLVSMAMDHPPGSS-SGDLQDRYQKWMNKYGRQYKCREEWE 60
           M W NV LI+LILWVFWTP LVSMAMD+  GSS S D+QDRYQKWM+KYGRQYK REEWE
Sbjct: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60

Query: 61  QRFTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNM 120
           +RFTIYQANVQYIDNFNS+N S+TLAENNFADLTNEEFK TYLGYKTVS+PDT FRYGNM
Sbjct: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120

Query: 121 VNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDV 180
           VNLPTNVDWR+EGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKL+SLSEQELVDCDV
Sbjct: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180

Query: 181 ASGNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPIN 240
            SGNQGC+GGYMYKAFEFIK+TGLTTE EYPY+G ESACN+QK KYQ VSI+GYEKVP+N
Sbjct: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVN 240

Query: 241 DEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWL 300
           DEKSLKAAVA QPVSVAIDA GN+FQFYSGGIFSGNCG QLNHGV IVGYGE +N+AYWL
Sbjct: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300

Query: 301 VKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
           VKNSWG DWGESGYIRMKRDSTD+QGTCGIAMMASYP KD
Sbjct: 301 VKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

BLAST of PI0005063 vs. ExPASy TrEMBL
Match: A0A384S0D9 (Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.4e-164
Identity = 276/319 (86.52%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 20  LVSMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSLNQ 79
           + SM MD+ PGSSSGDLQDRYQKWM+KYGR+YK REEWEQRF IYQ NVQYIDNFNSLN 
Sbjct: 1   MASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNH 60

Query: 80  SYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQ 139
           SYTLAEN+FADLTN+EFKTTYLG+KT  +PDT+FRYGNMVNLPTNVDWRKE AVTP+K+Q
Sbjct: 61  SYTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQ 120

Query: 140 GQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIKK 199
           GQCGSCWAFSAVAAVEGINKIK GKLMSLSEQELVDCDVASGNQGC+GGYMYKAFEFIKK
Sbjct: 121 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKK 180

Query: 200 TGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAG 259
           TGLTTE EYPY+G ES CNKQK +Y++V+I+GYEKVP+NDEKSLKAAVA QPVSVAIDAG
Sbjct: 181 TGLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAG 240

Query: 260 GNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWLVKNSWGNDWGESGYIRMKRDS 319
           G DFQFYSGG+FSGNCGKQLNHGV IVGYGE +NK YWLVKNSWG DWGESGYIRMKRDS
Sbjct: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNKTYWLVKNSWGTDWGESGYIRMKRDS 300

Query: 320 TDKQGTCGIAMMASYPIKD 339
           TDK+GTCGIAMMASYPIKD
Sbjct: 301 TDKRGTCGIAMMASYPIKD 319

BLAST of PI0005063 vs. ExPASy TrEMBL
Match: A0A6J1CH04 (ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.9e-154
Identity = 265/338 (78.40%), Postives = 297/338 (87.87%), Query Frame = 0

Query: 1   MIWNVGLIYLILWVFWTPSLVSMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQR 60
           MI NVG ++LIL VFWT S+ S+A D+PPG  S D++DRYQKW++KYGR+YK  EE E+R
Sbjct: 6   MIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKR 65

Query: 61  FTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNMVN 120
           F IYQ+NVQYID FNSLN+SYTLA+N FADLTN+EFKTTYLGY T   PDT F+YGN+VN
Sbjct: 66  FPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKYGNIVN 125

Query: 121 LPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVAS 180
           LPTNVDWRKEGAVTPIK+QGQCGSCWAFSAVAAVEGI KIK GKL+SLSEQEL+DCDV S
Sbjct: 126 LPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVIS 185

Query: 181 GNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDE 240
           GNQGCSGG+M KAFEFIKK G+TTE EYPY+G E+ CNKQK +Y S +I+GYEKVP NDE
Sbjct: 186 GNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDE 245

Query: 241 KSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWLVK 300
           KSLKAAVA QPVSVAIDAGG DFQFYSGGIFSGNCGKQLNHGVTIVGYGED  K+YWLVK
Sbjct: 246 KSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKSYWLVK 305

Query: 301 NSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
           NSWG  WGE GY+RMK +S+DK+GTCGIAM ASYPIKD
Sbjct: 306 NSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 343

BLAST of PI0005063 vs. NCBI nr
Match: XP_008458487.1 (PREDICTED: ervatamin-B-like [Cucumis melo] >KAA0033464.1 ervatamin-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 628.2 bits (1619), Expect = 4.0e-176
Identity = 305/341 (89.44%), Postives = 319/341 (93.55%), Query Frame = 0

Query: 1   MIWNVGLIYLILWVFWTPSLVSMAMDHPPGSS-SGDLQDRYQKWMNKYGRQYKCREEWEQ 60
           MIW+V LI LILWVFWTP+ VSMAMD+P GSS SG+LQDRYQKWM+KYGRQYK REEWEQ
Sbjct: 6   MIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWEQ 65

Query: 61  RFTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVP--DTFFRYGN 120
           RFTIYQANVQYIDNFNSLN SYTLAENNF DLTNEEF  TYLGY+TVS+P  DTFFRYGN
Sbjct: 66  RFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYGN 125

Query: 121 MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD 180
           MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD
Sbjct: 126 MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCD 185

Query: 181 VASGNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPI 240
           V SGNQGC+GGYMYKAFEFIKKTGLTTE EYPY  T SAC+KQK KYQSVSI+GYEKVP+
Sbjct: 186 VTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVPV 245

Query: 241 NDEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYW 300
           NDEKSL+AAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGV IVGYGED+N+AYW
Sbjct: 246 NDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQAYW 305

Query: 301 LVKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
           LVKNSWG  WGESGYIRM RDSTDKQGTCGIAMMASYPIKD
Sbjct: 306 LVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

BLAST of PI0005063 vs. NCBI nr
Match: KGN62053.1 (hypothetical protein Csa_006706 [Cucumis sativus])

HSP 1 Score: 614.0 bits (1582), Expect = 7.8e-172
Identity = 296/340 (87.06%), Postives = 316/340 (92.94%), Query Frame = 0

Query: 1   MIW-NVGLIYLILWVFWTPSLVSMAMDHPPGSS-SGDLQDRYQKWMNKYGRQYKCREEWE 60
           M W NV LI+LILWVFWTP LVSMAMD+  GSS S D+QDRYQKWM+KYGRQYK REEWE
Sbjct: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60

Query: 61  QRFTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNM 120
           +RFTIYQANVQYIDNFNS+N S+TLAENNFADLTNEEFK TYLGYKTVS+PDT FRYGNM
Sbjct: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120

Query: 121 VNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDV 180
           VNLPTNVDWR+EGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKL+SLSEQELVDCDV
Sbjct: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180

Query: 181 ASGNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPIN 240
            SGNQGC+GGYMYKAFEFIK+TGLTTE EYPY+G ESACN+QK KYQ VSI+GYEKVP+N
Sbjct: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVN 240

Query: 241 DEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWL 300
           DEKSLKAAVA QPVSVAIDA GN+FQFYSGGIFSGNCG QLNHGV IVGYGE +N+AYWL
Sbjct: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300

Query: 301 VKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
           VKNSWG DWGESGYIRMKRDSTD+QGTCGIAMMASYP KD
Sbjct: 301 VKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

BLAST of PI0005063 vs. NCBI nr
Match: XP_004148072.2 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 614.0 bits (1582), Expect = 7.8e-172
Identity = 296/340 (87.06%), Postives = 316/340 (92.94%), Query Frame = 0

Query: 1   MIW-NVGLIYLILWVFWTPSLVSMAMDHPPGSS-SGDLQDRYQKWMNKYGRQYKCREEWE 60
           M W NV LI+LILWVFWTP LVSMAMD+  GSS S D+QDRYQKWM+KYGRQYK REEWE
Sbjct: 17  MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 76

Query: 61  QRFTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNM 120
           +RFTIYQANVQYIDNFNS+N S+TLAENNFADLTNEEFK TYLGYKTVS+PDT FRYGNM
Sbjct: 77  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 136

Query: 121 VNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDV 180
           VNLPTNVDWR+EGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKL+SLSEQELVDCDV
Sbjct: 137 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 196

Query: 181 ASGNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPIN 240
            SGNQGC+GGYMYKAFEFIK+TGLTTE EYPY+G ESACN+QK KYQ VSI+GYEKVP+N
Sbjct: 197 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVN 256

Query: 241 DEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWL 300
           DEKSLKAAVA QPVSVAIDA GN+FQFYSGGIFSGNCG QLNHGV IVGYGE +N+AYWL
Sbjct: 257 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 316

Query: 301 VKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
           VKNSWG DWGESGYIRMKRDSTD+QGTCGIAMMASYP KD
Sbjct: 317 VKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 356

BLAST of PI0005063 vs. NCBI nr
Match: XP_038902939.1 (ervatamin-B [Benincasa hispida])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 290/338 (85.80%), Postives = 313/338 (92.60%), Query Frame = 0

Query: 1   MIWNVGLIYLILWVFWTPSLVSMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQR 60
           MIWNVGL+ LILWV WTP++V MAMD+PPGSSSGDLQ RYQKWM+KYGRQYK REEWE+R
Sbjct: 6   MIWNVGLMSLILWVIWTPTMVFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERR 65

Query: 61  FTIYQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNMVN 120
           FTIYQ NVQYIDNFNSL+ SYTLAENNFADLTN+EFK TYLGYKT  +PDT FRYGNMV+
Sbjct: 66  FTIYQLNVQYIDNFNSLDHSYTLAENNFADLTNDEFKETYLGYKTDWLPDTCFRYGNMVD 125

Query: 121 LPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVAS 180
           LPTNV+WRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIK GKLMSLSEQELVDCDVAS
Sbjct: 126 LPTNVNWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVAS 185

Query: 181 GNQGCSGGYMYKAFEFIKKTGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDE 240
           GNQGC+GG+M KAF+FIKKTGLTTETEYPY+G ES CNKQK +  +V I+GYEKVP NDE
Sbjct: 186 GNQGCNGGFMDKAFQFIKKTGLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDE 245

Query: 241 KSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWLVK 300
           KSLKAAVA QPVSVAIDAGG DFQFYSGG+FSGNCGKQLNHGV IVGYG+ +NK+YWLVK
Sbjct: 246 KSLKAAVANQPVSVAIDAGGYDFQFYSGGVFSGNCGKQLNHGVAIVGYGQASNKSYWLVK 305

Query: 301 NSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIKD 339
           NSWG DWGESGYIRMKRDSTDK+GTCGIAMMASYPIKD
Sbjct: 306 NSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 343

BLAST of PI0005063 vs. NCBI nr
Match: AKO60151.1 (cysteine proteinase 1, partial [Citrullus lanatus])

HSP 1 Score: 586.3 bits (1510), Expect = 1.7e-163
Identity = 276/319 (86.52%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 20  LVSMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSLNQ 79
           + SM MD+ PGSSSGDLQDRYQKWM+KYGR+YK REEWEQRF IYQ NVQYIDNFNSLN 
Sbjct: 1   MASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNH 60

Query: 80  SYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQ 139
           SYTLAEN+FADLTN+EFKTTYLG+KT  +PDT+FRYGNMVNLPTNVDWRKE AVTP+K+Q
Sbjct: 61  SYTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQ 120

Query: 140 GQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIKK 199
           GQCGSCWAFSAVAAVEGINKIK GKLMSLSEQELVDCDVASGNQGC+GGYMYKAFEFIKK
Sbjct: 121 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKK 180

Query: 200 TGLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAG 259
           TGLTTE EYPY+G ES CNKQK +Y++V+I+GYEKVP+NDEKSLKAAVA QPVSVAIDAG
Sbjct: 181 TGLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAG 240

Query: 260 GNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWLVKNSWGNDWGESGYIRMKRDS 319
           G DFQFYSGG+FSGNCGKQLNHGV IVGYGE +NK YWLVKNSWG DWGESGYIRMKRDS
Sbjct: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNKTYWLVKNSWGTDWGESGYIRMKRDS 300

Query: 320 TDKQGTCGIAMMASYPIKD 339
           TDK+GTCGIAMMASYPIKD
Sbjct: 301 TDKRGTCGIAMMASYPIKD 319

BLAST of PI0005063 vs. TAIR 10
Match: AT1G06260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 352.8 bits (904), Expect = 3.0e-97
Identity = 183/340 (53.82%), Postives = 233/340 (68.53%), Query Frame = 0

Query: 4   NVGLIYLILWVFWTPSLVSMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQRFTI 63
           N+ L  LI +V     L S  +D         L+ R++KW+  + + Y  R+EW  RF I
Sbjct: 9   NLTLAVLICFVLIASKLCS--VDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGI 68

Query: 64  YQANVQYIDNFNSLNQSYTLAENNFADLTNEEFKTTYLGYKTVSVPDTFFRYGNMV---- 123
           YQ+NVQ ID  NSL+  + L +N FAD+TN EFK  +LG  T S+     +    V    
Sbjct: 69  YQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSL--RLHKKQRPVCDPA 128

Query: 124 -NLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDV 183
            N+P  VDWR +GAVTPI+NQG+CG CWAFSAVAA+EGINKIK G L+SLSEQ+L+DCDV
Sbjct: 129 GNVPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCDV 188

Query: 184 ASGNQGCSGGYMYKAFEFIKKT-GLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPI 243
            + N+GCSGG M  AFEFIK   GL TET+YPY G E  C+++K+K + V+I GY+KV  
Sbjct: 189 GTYNKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGTCDQEKSKNKVVTIQGYQKVAQ 248

Query: 244 NDEKSLKAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYW 303
           N E SL+ A A+QPVSV IDAGG  FQ YS G+F+  CG  LNHGVT+VGYG + ++ YW
Sbjct: 249 N-EASLQIAAAQQPVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYW 308

Query: 304 LVKNSWGNDWGESGYIRMKRDSTDKQGTCGIAMMASYPIK 338
           +VKNSWG  WGE GYIRM+R  ++  G CGIAMMASYP++
Sbjct: 309 IVKNSWGTGWGEEGYIRMERGVSEDTGKCGIAMMASYPLQ 343

BLAST of PI0005063 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 346.7 bits (888), Expect = 2.2e-95
Identity = 174/313 (55.59%), Postives = 226/313 (72.20%), Query Frame = 0

Query: 36  LQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSL--NQSYTLAENNFADLTN 95
           +Q R+ +WM K+GR Y   +E   R+ +++ NV+ I++ NS+   +++ LA N FADLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 96  EEFKTTYLGYKTVSVPDTF-------FRYGNMVN--LPTNVDWRKEGAVTPIKNQGQCGS 155
           +EF++ Y G+K VS   +        FRY N+ +  LP +VDWRK+GAVTPIKNQG CG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGC 153

Query: 156 CWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIKKT-GLT 215
           CWAFSAVAA+EG  +IK GKL+SLSEQ+LVDCD  + + GC GG M  AFE IK T GLT
Sbjct: 154 CWAFSAVAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATGGLT 213

Query: 216 TETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAGGNDF 275
           TE+ YPYKG ++ CN +K   ++ SITGYE VP+NDE++L  AVA QPVSV I+ GG DF
Sbjct: 214 TESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDF 273

Query: 276 QFYSGGIFSGNCGKQLNHGVTIVGYGEDTN-KAYWLVKNSWGNDWGESGYIRMKRDSTDK 335
           QFYS G+F+G C   L+H VT +GYGE TN   YW++KNSWG  WGESGY+R+++D  DK
Sbjct: 274 QFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDK 333

BLAST of PI0005063 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 2.5e-91
Identity = 172/317 (54.26%), Postives = 215/317 (67.82%), Query Frame = 0

Query: 32  SSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSLNQSYTLAENNFADL 91
           S   L + Y++W + +    +  EE  +RF +++ NV++I   N  ++SY L  N F D+
Sbjct: 30  SENSLWELYERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDM 89

Query: 92  TNEEFKTTYLG--------YKTVSVPDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCG 151
           T+EEF+ TY G        ++        F Y N+  LPT+VDWRK GAVTP+KNQGQCG
Sbjct: 90  TSEEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCG 149

Query: 152 SCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIK-KTGL 211
           SCWAFS V AVEGIN+I+  KL SLSEQELVDCD  + NQGC+GG M  AFEFIK K GL
Sbjct: 150 SCWAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGL 209

Query: 212 TTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAGGND 271
           T+E  YPYK ++  C+  K     VSI G+E VP N E  L  AVA QPVSVAIDAGG+D
Sbjct: 210 TSELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSD 269

Query: 272 FQFYSGGIFSGNCGKQLNHGVTIVGYGEDTN-KAYWLVKNSWGNDWGESGYIRMKRDSTD 331
           FQFYS G+F+G CG +LNHGV +VGYG   +   YW+VKNSWG +WGE GYIRM+R    
Sbjct: 270 FQFYSEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRH 329

Query: 332 KQGTCGIAMMASYPIKD 339
           K+G CGIAM ASYP+K+
Sbjct: 330 KEGLCGIAMEASYPLKN 344

BLAST of PI0005063 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 3.2e-91
Identity = 170/317 (53.63%), Postives = 215/317 (67.82%), Query Frame = 0

Query: 32  SSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSLNQSYTLAENNFADL 91
           S   L   Y +W + +       E  E+RF +++ NV ++ N N  N+SY L  N FADL
Sbjct: 30  SEEGLSTLYDRWRSHHSVPRSLNER-EKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADL 89

Query: 92  TNEEFKTTYLG-----YKTVSVP-----DTFFRYGNMVNLPTNVDWRKEGAVTPIKNQGQ 151
           T  EFK  Y G     ++ +  P        + + N+  LP++VDWRK+GAVT IKNQG+
Sbjct: 90  TINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGK 149

Query: 152 CGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKAFEFIKKT- 211
           CGSCWAFS VAAVEGINKIK  KL+SLSEQELVDCD    N+GC+GG M  AFEFIKK  
Sbjct: 150 CGSCWAFSTVAAVEGINKIKTNKLVSLSEQELVDCDTKQ-NEGCNGGLMEIAFEFIKKNG 209

Query: 212 GLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPVSVAIDAGG 271
           G+TTE  YPY+G +  C+  K     V+I G+E VP NDE +L  AVA QPVSVAIDAG 
Sbjct: 210 GITTEDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGS 269

Query: 272 NDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWLVKNSWGNDWGESGYIRMKRDST 331
           +DFQFYS G+F+G+CG +LNHGV  VGYG +  K YW+V+NSWG +WGE GYI+++R+  
Sbjct: 270 SDFQFYSEGVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREID 329

Query: 332 DKQGTCGIAMMASYPIK 338
           + +G CGIAM ASYPIK
Sbjct: 330 EPEGRCGIAMEASYPIK 344

BLAST of PI0005063 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 325.9 bits (834), Expect = 3.9e-89
Identity = 167/325 (51.38%), Postives = 217/325 (66.77%), Query Frame = 0

Query: 19  SLVSMAMDHPPGSSSGDLQDRYQKWMNKYGRQYKCREEWEQRFTIYQANVQYIDNFNSLN 78
           S+V    +H   +++  L + ++ WM+++ + YK  EE   RF +++ N+ +ID  N+  
Sbjct: 32  SIVGYTPEHL--TNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEI 91

Query: 79  QSYTLAENNFADLTNEEFKTTYLG-----YKTVSVPDTFFRYGNMVNLPTNVDWRKEGAV 138
            SY L  N FADLT+EEFK  YLG     +     P   FRY ++ +LP +VDWRK+GAV
Sbjct: 92  NSYWLGLNEFADLTHEEFKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAV 151

Query: 139 TPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVASGNQGCSGGYMYKA 198
            P+K+QGQCGSCWAFS VAAVEGIN+I  G L SLSEQEL+DCD  + N GC+GG M  A
Sbjct: 152 APVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYA 211

Query: 199 FEFIKKT-GLTTETEYPYKGTESACNKQKAKYQSVSITGYEKVPINDEKSLKAAVAKQPV 258
           F++I  T GL  E +YPY   E  C +QK   + V+I+GYE VP ND++SL  A+A QPV
Sbjct: 212 FQYIISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPV 271

Query: 259 SVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDTNKAYWLVKNSWGNDWGESGY 318
           SVAI+A G DFQFY GG+F+G CG  L+HGV  VGYG      Y +VKNSWG  WGE G+
Sbjct: 272 SVAIEASGRDFQFYKGGVFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGF 331

Query: 319 IRMKRDSTDKQGTCGIAMMASYPIK 338
           IRMKR++   +G CGI  MASYP K
Sbjct: 332 IRMKRNTGKPEGLCGINKMASYPTK 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJ473.0e-9455.59Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana OX=3702 GN=S... [more]
P124127.5e-9352.33Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
P258031.3e-9252.33Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
Q9FGR93.5e-9054.26KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ... [more]
Q9STL44.5e-9053.63KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ... [more]
Match NameE-valueIdentityDescription
A0A5A7SQK01.9e-17689.44Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001... [more]
A0A1S3C8281.9e-17689.44ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1[more]
A0A0A0LJV63.8e-17287.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292830 PE=3 SV=1[more]
A0A384S0D98.4e-16486.52Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1[more]
A0A6J1CH047.9e-15478.40ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008458487.14.0e-17689.44PREDICTED: ervatamin-B-like [Cucumis melo] >KAA0033464.1 ervatamin-B-like [Cucum... [more]
KGN62053.17.8e-17287.06hypothetical protein Csa_006706 [Cucumis sativus][more]
XP_004148072.27.8e-17287.06ervatamin-B [Cucumis sativus][more]
XP_038902939.11.1e-17085.80ervatamin-B [Benincasa hispida][more]
AKO60151.11.7e-16386.52cysteine proteinase 1, partial [Citrullus lanatus][more]
Match NameE-valueIdentityDescription
AT1G06260.13.0e-9753.82Cysteine proteinases superfamily protein [more]
AT5G45890.12.2e-9555.59senescence-associated gene 12 [more]
AT5G50260.12.5e-9154.26Cysteine proteinases superfamily protein [more]
AT3G48340.13.2e-9153.63Cysteine proteinases superfamily protein [more]
AT4G35350.13.9e-8951.38xylem cysteine peptidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 139..154
score: 67.03
coord: 281..291
score: 56.16
coord: 296..302
score: 75.76
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 121..336
e-value: 1.8E-118
score: 409.6
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 121..336
e-value: 1.2E-83
score: 280.5
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 40..96
e-value: 1.7E-21
score: 87.4
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 40..96
e-value: 9.9E-14
score: 51.5
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 23..338
e-value: 1.2E-119
score: 401.6
NoneNo IPR availablePANTHERPTHR12411:SF796ERVATAMIN-B-LIKEcoord: 31..337
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 31..337
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 279..289
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 296..315
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 139..150
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 122..335
e-value: 2.11417E-109
score: 315.718
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 36..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005063.1PI0005063.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity