PI0004862 (gene) Melon (PI 482460) v1

Overview


Name	PI0004862
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr03: 11006544 .. 11007680 (+)
RNA-Seq Expression	PI0004862
Synteny	PI0004862

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCACAATGAAACTGATGGGCAAGTCTCACGCAGACCGATTGGTAGAGATCAAAGAGCAGATGCTCTACCTGGTGGAAGTTTCGAACGTCGTCCACTTCTTGGAAAGTCGAATTGAAGAAATTTCTGAGAAAGCTGACGCTATTGATGCAGTAACAGATCGTCTCGACAGATTACCTATAAATGAGTTGTTGACAAGAGTCGACACTCTAGAGGCAAAAGCTGCAAGAACTGGTAACTACGAGCGTGGGGACAGTTCAACGGGCTCTGTTGCCCATATAGAAGAACAGGTTGGAGAGTTAGATAACTCCCAAAGTCGATTGTTGAGATGATAAACTACATGTCAGAGGATTTTCGTGCCGCTCTTGACGTCGTCAGGAATGAGATCGTAGACGCGAGCACGAGGGTGAACCTCACGATGCGAGCCATGGCAAAGCAAGCTCCAACTGGAGGAGCAATACAAGTTAAGAGGGTGAAGAGTCCCTGAACCTAAACCCTTCTGTGGGGCAAGGGATGCAAATGCCTTGGAGAACTTCATATTTGACCTCGAACAATATTTTCGGGCCACAATCACAGTTGCAGAAGAAGCGAAAGTGACACTAGCAACGATACATCTAGCTGAGGATGCAAAGTTGTGGTGGAGGTTTTGGTATGCCGAGATCCAAGAAGGACGATGTACGATAGACATCTGGGACAGTTTAAAGAAAGAGCTACGCTCACAGTTCTTCCCCAAGAATGTTGAAATCCTTGCTCGACGGAAGTTACGTGAACTAAAACACAACGGTAGAATTCGAGATTATGTGAAGCAGTTTGCGGGACTGATGTTAGACATAAGCGATATGTCCGAGAAAGACAAAGTTTTTTGTTTTGTCGAAGGCCTTAAACCATGGGCGAAGACCAAGCTATACGAACAGAGGGTCCAAGAACTCTCGTCGGTATACCCAGCGACAGAGCAACTATTTGAGCTGTCCAATGACTCTCAAGATGCGAGACGTCATCCAAGTTCCTCATCTAGAGGAAACAAGAACAATCGTGCGAGTTCTCCAAAGGTCAGAGGGGGAGATAAACGATCTAATGGTGATCGTAGGCCTTTTCAACCAAACACCGGGAACACGGGGCGAGGACAGAACCCCTAA

mRNA sequence

ATGTCCACAATGAAACTGATGGGCAAGTCTCACGCAGACCGATTGGTAGAGATCAAAGAGCAGATGCTCTACCTGGTGGAAGTTTCGAACGTCGTCCACTTCTTGGAAAGTCGAATTGAAGAAATTTCTGAGAAAGCTGACGCTATTGATGCAGTAACAGATCGTCTCGACAGATTACCTATAAATGAGTTGTTGACAAGAGTCGACACTCTAGAGGCAAAAGCTGCAAGAACTGGCCTTAAACCATGGGCGAAGACCAAGCTATACGAACAGAGGGTCCAAGAACTCTCGTCGGTATACCCAGCGACAGAGCAACTATTTGAGCTGTCCAATGACTCTCAAGATGCGAGACGTCATCCAAGTTCCTCATCTAGAGGAAACAAGAACAATCGTGCGAGTTCTCCAAAGGTCAGAGGGGGAGATAAACGATCTAATGGTGATCGTAGGCCTTTTCAACCAAACACCGGGAACACGGGGCGAGGACAGAACCCCTAA

Coding sequence (CDS)

ATGTCCACAATGAAACTGATGGGCAAGTCTCACGCAGACCGATTGGTAGAGATCAAAGAGCAGATGCTCTACCTGGTGGAAGTTTCGAACGTCGTCCACTTCTTGGAAAGTCGAATTGAAGAAATTTCTGAGAAAGCTGACGCTATTGATGCAGTAACAGATCGTCTCGACAGATTACCTATAAATGAGTTGTTGACAAGAGTCGACACTCTAGAGGCAAAAGCTGCAAGAACTGGCCTTAAACCATGGGCGAAGACCAAGCTATACGAACAGAGGGTCCAAGAACTCTCGTCGGTATACCCAGCGACAGAGCAACTATTTGAGCTGTCCAATGACTCTCAAGATGCGAGACGTCATCCAAGTTCCTCATCTAGAGGAAACAAGAACAATCGTGCGAGTTCTCCAAAGGTCAGAGGGGGAGATAAACGATCTAATGGTGATCGTAGGCCTTTTCAACCAAACACCGGGAACACGGGGCGAGGACAGAACCCCTAA

Protein sequence

MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLPINELLTRVDTLEAKAARTGLKPWAKTKLYEQRVQELSSVYPATEQLFELSNDSQDARRHPSSSSRGNKNNRASSPKVRGGDKRSNGDRRPFQPNTGNTGRGQNP

Homology

[Close]

BLAST of PI0004862 vs. ExPASy TrEMBL
Match: A0A5A7ULF5 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1428G00300 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 5.9e-30
Identity = 103/275 (37.45%), Postives = 122/275 (44.36%), Query Frame = 0


Query: 1   MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLP 60
           MS+    GK+  DRLVE++EQMLYLVEV + + FLESR+EEI+EK D ID V  R++ L 
Sbjct: 1   MSSANPSGKAQRDRLVELEEQMLYLVEVPDSIRFLESRLEEIAEKNDMIDPVAGRVEGLS 60

Query: 61  INELLTRVDTLEAKAART------------------------------------------ 120
           I ELL RVDTLE    RT                                          
Sbjct: 61  IQELLARVDTLEGTVGRTGSHERGDSSTGSVAHIEERVQELDNSQKTLLEMINDMSEDFR 120

Query: 121 ------------------------------------------------------------ 159
                                                                       
Sbjct: 121 ATLDVEGRCTIDTWDDLKRELCSQFFPENVEILARRKLRELKHTGSIREYVKRFMGLMLD 180

[Close]

BLAST of PI0004862 vs. ExPASy TrEMBL
Match: A0A5D3DEJ3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G00860 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.5e-28
Identity = 105/298 (35.23%), Postives = 127/298 (42.62%), Query Frame = 0


Query: 1   MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLP 60
           MS++   GK+  DRLVE++EQMLYLVEV + + +LES ++EISEK + IDAV  R++  P
Sbjct: 1   MSSLNPSGKAQKDRLVELEEQMLYLVEVLDSIRYLESHLDEISEKTNTIDAVAGRVEGFP 60

Query: 61  INELLTRVDTLEA----------------------------------------------- 120
           I EL+TRVD LE                                                
Sbjct: 61  IQELMTRVDALETTINIGRTVNYERGDSSTSSVAHIEERVQELDSSQKTLLEMINSMSED 120

Query: 121 --------------------------------------KAART----------------- 162
                                                 KA  T                 
Sbjct: 121 FRATLNVVRNEIADVNARLSLTIREGLENYIFDLEQYFKATNTVTEEAKVTLAMMHLSED 180

[Close]

BLAST of PI0004862 vs. ExPASy TrEMBL
Match: A0A5A7UYM0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G001390 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.5e-28
Identity = 105/298 (35.23%), Postives = 127/298 (42.62%), Query Frame = 0


Query: 1   MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLP 60
           MS++   GK+  DRLVE++EQMLYLVEV + + +LES ++EISEK + IDAV  R++  P
Sbjct: 1   MSSLNPSGKAQKDRLVELEEQMLYLVEVLDSIRYLESHLDEISEKTNTIDAVAGRVEGFP 60

Query: 61  INELLTRVDTLEA----------------------------------------------- 120
           I EL+TRVD LE                                                
Sbjct: 61  IQELMTRVDALETTINIGRTVNYERGDSSTSSVAHIEERVQELDSSQKTLLEMINSMSED 120

Query: 121 --------------------------------------KAART----------------- 162
                                                 KA  T                 
Sbjct: 121 FRATLNVVRNEIADVNARLSLTIREGLENYIFDLEQYFKATNTVTEEAKVTLAMMHLSED 180

[Close]

BLAST of PI0004862 vs. ExPASy TrEMBL
Match: A0A5A7TEV3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G001730 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 7.2e-28
Identity = 98/273 (35.90%), Postives = 120/273 (43.96%), Query Frame = 0


Query: 1   MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLP 60
           MS++   GK+  DRLVE++EQMLYLVEV + +HFLES +EEI+EK D IDA+  R++ LP
Sbjct: 1   MSSVNPSGKAQRDRLVELEEQMLYLVEVPDSIHFLESHLEEIAEKTDMIDAIAGRVEGLP 60

Query: 61  INELLTRVDTLEAKAART------------------------------------------ 120
           I +LL RVDTLE    RT                                          
Sbjct: 61  IQKLLARVDTLEGTVGRTGSHERGDSSTGSVAHIEERVQELDSSQKKLLEMINYMSEDFL 120

Query: 121 ------------------------------------------------------------ 149
                                                                       
Sbjct: 121 ATLDVVRNEIADVNARLSLTMRAMANLGRSTICGGPDLLTCRRDKLCELKHIGSIREYVK 180

[Close]

BLAST of PI0004862 vs. ExPASy TrEMBL
Match: A0A5A7USX3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005730 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.4e-25
Identity = 88/213 (41.31%), Postives = 105/213 (49.30%), Query Frame = 0


Query: 22  MLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLPINELLTRVDTLEAKAART--- 81
           MLYL +V + V FLESR EEI+EK D ID V  R++ LPI ELL RVDTLE    RT   
Sbjct: 1   MLYLAKVPDSVRFLESRFEEIAEKTDTIDTVASRVEGLPIQELLARVDTLEGNVGRTVNY 60

Query: 82  ------------------------------------------------------------ 141
                                                                       
Sbjct: 61  EYGDSSSGFVGHMEEHVKKATNTVTEEAKVTLATMHLSEDAKLVEVPFFPENGEILARRK 120

Query: 142 --------GLKPWAKTKLYEQRVQELSSVYPATEQLFELSNDSQDARRHPSSSSRGNKNN 164
                    LKPWAKTKLYEQRVQ+L+S Y ATE+L +L +DSQD RRH SSS + N+N+
Sbjct: 121 LCELKHTDRLKPWAKTKLYEQRVQDLTSTYAATERLSDLISDSQDVRRHQSSSPQRNRNS 180

[Close]

BLAST of PI0004862 vs. NCBI nr
Match: KAA0055407.1 (uncharacterized protein E6C27_scaffold80G002530 [Cucumis melo var. makuwa] >TYK22320.1 uncharacterized protein E5676_scaffold1428G00300 [Cucumis melo var. makuwa])

HSP 1 Score: 140.6 bits (353), Expect = 1.2e-29
Identity = 103/275 (37.45%), Postives = 122/275 (44.36%), Query Frame = 0


Query: 1   MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLP 60
           MS+    GK+  DRLVE++EQMLYLVEV + + FLESR+EEI+EK D ID V  R++ L 
Sbjct: 1   MSSANPSGKAQRDRLVELEEQMLYLVEVPDSIRFLESRLEEIAEKNDMIDPVAGRVEGLS 60

Query: 61  INELLTRVDTLEAKAART------------------------------------------ 120
           I ELL RVDTLE    RT                                          
Sbjct: 61  IQELLARVDTLEGTVGRTGSHERGDSSTGSVAHIEERVQELDNSQKTLLEMINDMSEDFR 120

Query: 121 ------------------------------------------------------------ 159
                                                                       
Sbjct: 121 ATLDVEGRCTIDTWDDLKRELCSQFFPENVEILARRKLRELKHTGSIREYVKRFMGLMLD 180

[Close]

BLAST of PI0004862 vs. NCBI nr
Match: KAA0060348.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 136.0 bits (341), Expect = 3.0e-28
Identity = 105/298 (35.23%), Postives = 127/298 (42.62%), Query Frame = 0


Query: 1   MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLP 60
           MS++   GK+  DRLVE++EQMLYLVEV + + +LES ++EISEK + IDAV  R++  P
Sbjct: 1   MSSLNPSGKAQKDRLVELEEQMLYLVEVLDSIRYLESHLDEISEKTNTIDAVAGRVEGFP 60

Query: 61  INELLTRVDTLEA----------------------------------------------- 120
           I EL+TRVD LE                                                
Sbjct: 61  IQELMTRVDALETTINIGRTVNYERGDSSTSSVAHIEERVQELDSSQKTLLEMINSMSED 120

Query: 121 --------------------------------------KAART----------------- 162
                                                 KA  T                 
Sbjct: 121 FRATLNVVRNEIADVNARLSLTIREGLENYIFDLEQYFKATNTVTEEAKVTLAMMHLSED 180

[Close]

BLAST of PI0004862 vs. NCBI nr
Match: TYK22097.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 136.0 bits (341), Expect = 3.0e-28
Identity = 105/298 (35.23%), Postives = 127/298 (42.62%), Query Frame = 0


Query: 1   MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLP 60
           MS++   GK+  DRLVE++EQMLYLVEV + + +LES ++EISEK + IDAV  R++  P
Sbjct: 1   MSSLNPSGKAQKDRLVELEEQMLYLVEVLDSIRYLESHLDEISEKTNTIDAVAGRVEGFP 60

Query: 61  INELLTRVDTLEA----------------------------------------------- 120
           I EL+TRVD LE                                                
Sbjct: 61  IQELMTRVDALETTINIGRTVNYERGDSSTSSVAHIEERVQELDSSQKTLLEMINSMSED 120

Query: 121 --------------------------------------KAART----------------- 162
                                                 KA  T                 
Sbjct: 121 FRATLNVVRNEIADVNARLSLTIREGLENYIFDLEQYFKATNTVTEEAKVTLAMMHLSED 180

[Close]

BLAST of PI0004862 vs. NCBI nr
Match: KAA0041962.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK17897.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 133.7 bits (335), Expect = 1.5e-27
Identity = 98/273 (35.90%), Postives = 120/273 (43.96%), Query Frame = 0


Query: 1   MSTMKLMGKSHADRLVEIKEQMLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLP 60
           MS++   GK+  DRLVE++EQMLYLVEV + +HFLES +EEI+EK D IDA+  R++ LP
Sbjct: 1   MSSVNPSGKAQRDRLVELEEQMLYLVEVPDSIHFLESHLEEIAEKTDMIDAIAGRVEGLP 60

Query: 61  INELLTRVDTLEAKAART------------------------------------------ 120
           I +LL RVDTLE    RT                                          
Sbjct: 61  IQKLLARVDTLEGTVGRTGSHERGDSSTGSVAHIEERVQELDSSQKKLLEMINYMSEDFL 120

Query: 121 ------------------------------------------------------------ 149
                                                                       
Sbjct: 121 ATLDVVRNEIADVNARLSLTMRAMANLGRSTICGGPDLLTCRRDKLCELKHIGSIREYVK 180

[Close]

BLAST of PI0004862 vs. NCBI nr
Match: KAA0058248.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 124.8 bits (312), Expect = 6.9e-25
Identity = 88/213 (41.31%), Postives = 105/213 (49.30%), Query Frame = 0


Query: 22  MLYLVEVSNVVHFLESRIEEISEKADAIDAVTDRLDRLPINELLTRVDTLEAKAART--- 81
           MLYL +V + V FLESR EEI+EK D ID V  R++ LPI ELL RVDTLE    RT   
Sbjct: 1   MLYLAKVPDSVRFLESRFEEIAEKTDTIDTVASRVEGLPIQELLARVDTLEGNVGRTVNY 60

Query: 82  ------------------------------------------------------------ 141
                                                                       
Sbjct: 61  EYGDSSSGFVGHMEEHVKKATNTVTEEAKVTLATMHLSEDAKLVEVPFFPENGEILARRK 120

Query: 142 --------GLKPWAKTKLYEQRVQELSSVYPATEQLFELSNDSQDARRHPSSSSRGNKNN 164
                    LKPWAKTKLYEQRVQ+L+S Y ATE+L +L +DSQD RRH SSS + N+N+
Sbjct: 121 LCELKHTDRLKPWAKTKLYEQRVQDLTSTYAATERLSDLISDSQDVRRHQSSSPQRNRNS 180

The following BLAST results are available for this feature:

BLAST of PI0004862 vs.


Match Name	E-value	Identity	Description

BLAST of PI0004862 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7ULF5	5.9e-30	37.45	Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A5D3DEJ3	1.5e-28	35.23	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold31...	[more]
A0A5A7UYM0	1.5e-28	35.23	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22...	[more]
A0A5A7TEV3	7.2e-28	35.90	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold30...	[more]
A0A5A7USX3	3.4e-25	41.31	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27...	[more]

BLAST of PI0004862 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0055407.1	1.2e-29	37.45	uncharacterized protein E6C27_scaffold80G002530 [Cucumis melo var. makuwa] >TYK2...	[more]
KAA0060348.1	3.0e-28	35.23	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK22097.1	3.0e-28	35.23	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0041962.1	1.5e-27	35.90	reverse transcriptase [Cucumis melo var. makuwa] >TYK17897.1 reverse transcripta...	[more]
KAA0058248.1	6.9e-25	41.31	reverse transcriptase [Cucumis melo var. makuwa]	[more]

BLAST of PI0004862 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 149..164
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 107..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0004862.1	PI0004862.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0008270	zinc ion binding