PI0004326 (gene) Melon (PI 482460) v1

Overview
NamePI0004326
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPoly(A)-specific ribonuclease
Locationchr03: 4552488 .. 4553311 (-)
RNA-Seq ExpressionPI0004326
SyntenyPI0004326
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGATTTTGCCGAAAGGTGAATCGATTAATATCCGTGAGGTGTGGAACGATAATTTGGAGGAAGAATTTGCTCTTATACGTGAAATTGTCGACCAGTTTAACTACATAGCGATGGATACCGAGTTCCCTGGCGTGGTTGTTCGTCCTGTGGGTTCTTTTAAGAATATTAATGAGTATAACTACCGAACCCTCAGAGAGAATGTCGATACCTTAAAGTTGATTCAATTGGGTCTTACGTTTTCTGATGAGAATGGGAATCTCCCAACTTGTGGAACAGATAAGTTTTGTATTTGGCAGTTCAATTTCCGCGAGTTCAATATTTGTGATGATATTTATGCAAGTGATTCCATTGAGCTTTTGCGCGAGTGTGGTATTGATTTTCAGAAGACCCATGAGGAGGGAATTGATGTCAATCGCTTTGGTGAGCTTCTCATGTCATCTGGGATTGTGTTAAACGATAATATTTTTGGGTGACGTTCCACAGCGGGTATGATTTTGGGTACTTGTTGAAATTGTTGACTTGCCGAAACTTACCAGAGACCCAGGCCGAATTTTTTGATTTGATCCATATGTACTTCCCAATGGTTTATGATATCAAGCATTTGATGAGGTTTTGCAACAGTTTCCATGGTGGGCTGAACAAGCTTGCAGAGTTATTAGAAGTGGAGAGGGTTGGAGTTTGCCATCAAGCAGGATCAGATAGCCTCCTTACTGCCTGTACTTTCAGGAAGTTGAGGGATACTTTTTTCAATGGTTCTACACAGAAATATGCAGGTGTTTTATATGGTCTCGGTGTTGAAACTGGACAGACTACCAATTGA

mRNA sequence

ATGTCGATTTTGCCGAAAGGTGAATCGATTAATATCCGTGAGGTGTGGAACGATAATTTGGAGGAAGAATTTGCTCTTATACGTGAAATTGTCGACCAGTTTAACTACATAGCGATGGATACCGAGTTCCCTGGCGTGGTTGTTCGTCCTGTGGGTTCTTTTAAGAATATTAATGAGTATAACTACCGAACCCTCAGAGAGAATGTCGATACCTTAAAGTTGATTCAATTGGGTCTTACGTTTTCTGATGAGAATGGGAATCTCCCAACTTGTGGAACAGATAAGTTTTGTATTTGGCAGTTCAATTTCCGCGAGTTCAATATTTGTGATGATATTTATGCAAGTGATTCCATTGAGCTTTTGCGCGAGTGTGGTATTGATTTTCAGAAGACCCATGAGGAGGGAATTGATGTCAATCGCTTTGGTGAGCTTCTCATGTCATCTGGGATTGTCGGGTATGATTTTGGGTACTTGTTGAAATTGTTGACTTGCCGAAACTTACCAGAGACCCAGGCCGAATTTTTTGATTTGATCCATATGTACTTCCCAATGGTTTATGATATCAAGCATTTGATGAGGTTTTGCAACAGTTTCCATGGTGGGCTGAACAAGCTTGCAGAGTTATTAGAAGTGGAGAGGGTTGGAGTTTGCCATCAAGCAGGATCAGATAGCCTCCTTACTGCCTGTACTTTCAGGAAGTTGAGGGATACTTTTTTCAATGGTTCTACACAGAAATATGCAGGTGTTTTATATGGTCTCGGTGTTGAAACTGGACAGACTACCAATTGA

Coding sequence (CDS)

ATGTCGATTTTGCCGAAAGGTGAATCGATTAATATCCGTGAGGTGTGGAACGATAATTTGGAGGAAGAATTTGCTCTTATACGTGAAATTGTCGACCAGTTTAACTACATAGCGATGGATACCGAGTTCCCTGGCGTGGTTGTTCGTCCTGTGGGTTCTTTTAAGAATATTAATGAGTATAACTACCGAACCCTCAGAGAGAATGTCGATACCTTAAAGTTGATTCAATTGGGTCTTACGTTTTCTGATGAGAATGGGAATCTCCCAACTTGTGGAACAGATAAGTTTTGTATTTGGCAGTTCAATTTCCGCGAGTTCAATATTTGTGATGATATTTATGCAAGTGATTCCATTGAGCTTTTGCGCGAGTGTGGTATTGATTTTCAGAAGACCCATGAGGAGGGAATTGATGTCAATCGCTTTGGTGAGCTTCTCATGTCATCTGGGATTGTCGGGTATGATTTTGGGTACTTGTTGAAATTGTTGACTTGCCGAAACTTACCAGAGACCCAGGCCGAATTTTTTGATTTGATCCATATGTACTTCCCAATGGTTTATGATATCAAGCATTTGATGAGGTTTTGCAACAGTTTCCATGGTGGGCTGAACAAGCTTGCAGAGTTATTAGAAGTGGAGAGGGTTGGAGTTTGCCATCAAGCAGGATCAGATAGCCTCCTTACTGCCTGTACTTTCAGGAAGTTGAGGGATACTTTTTTCAATGGTTCTACACAGAAATATGCAGGTGTTTTATATGGTCTCGGTGTTGAAACTGGACAGACTACCAATTGA

Protein sequence

MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEYNYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELLRECGIDFQKTHEEGIDVNRFGELLMSSGIVGYDFGYLLKLLTCRNLPETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTACTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Homology
BLAST of PI0004326 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 408.3 bits (1048), Expect = 6.6e-113
Identity = 196/272 (72.06%), Postives = 225/272 (82.72%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNLE E ALIRE+VD F ++AMDTEFPG+V RPVG+FK   EY
Sbjct: 1   MSLFLKDDSIQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRPVGTFKTNTEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTD-KFCIWQFNFREFNICDDIYASDSIE 120
           +Y TL+ NV+ LK+IQLGLTFSDE GNLPTCGTD K+CIWQFNFREF++  DIYA+DSIE
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE 120

Query: 121 LLRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNL 180
           LLR+ GIDF K +E GID  RF ELLMSSGIV            GYDFGYLLKLLTC+NL
Sbjct: 121 LLRQSGIDFVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 180

Query: 181 PETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLT 240
           PETQ  FF++I +YFP VYDIKHLM+FCNS HGGLNKLAELL+VERVG+CHQAGSDSLLT
Sbjct: 181 PETQTGFFEMISVYFPRVYDIKHLMKFCNSLHGGLNKLAELLDVERVGICHQAGSDSLLT 240

Query: 241 ACTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 260
           +CTFRKL++ FF GS +KY+GVLYGLGVE GQ
Sbjct: 241 SCTFRKLQENFFIGSMEKYSGVLYGLGVENGQ 272

BLAST of PI0004326 vs. ExPASy Swiss-Prot
Match: Q9LEU4 (Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=CAF1-10 PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 4.3e-112
Identity = 196/268 (73.13%), Postives = 226/268 (84.33%), Query Frame = 0

Query: 6   KGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEYNYRTL 65
           K +SI IREVW+ NL EEFALIREIVD+F+YIAMDTEFPGVV++PV +FK  N+ NYRTL
Sbjct: 6   KEDSIMIREVWDYNLVEEFALIREIVDKFSYIAMDTEFPGVVLKPVATFKYNNDLNYRTL 65

Query: 66  RENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELLRECG 125
           +ENVD LKLIQ+GLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DIYAS+SIELLR+CG
Sbjct: 66  KENVDLLKLIQVGLTFSDENGNLPTCGTDKFCIWQFNFREFNIGEDIYASESIELLRQCG 125

Query: 126 IDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLPETQAE 185
           IDF+K  E+GIDV RFGEL+MSSGIV            GYDFGYL+KLLTC+ LP  QA+
Sbjct: 126 IDFKKNIEKGIDVVRFGELMMSSGIVLNDAISWVTFHGGYDFGYLVKLLTCKELPLKQAD 185

Query: 186 FFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTACTFRK 245
           FF L+++YFP VYDIKHLM FCN   GGLN+LAEL+ VERVG+CHQAGSDSLLT  +FRK
Sbjct: 186 FFKLLYVYFPTVYDIKHLMTFCNGLFGGLNRLAELMGVERVGICHQAGSDSLLTLGSFRK 245

Query: 246 LRDTFFNGSTQKYAGVLYGLGVETGQTT 262
           L++ +F GST+KY GVLYGLGVE G TT
Sbjct: 246 LKERYFPGSTEKYTGVLYGLGVEDGTTT 273

BLAST of PI0004326 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-110
Identity = 189/271 (69.74%), Postives = 223/271 (82.29%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNL+EE  LIR++VD F Y+AMDTEFPG+VVRPVG+FK+  +Y
Sbjct: 1   MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           +Y TL+ NV+ LK+IQLGLTFS+E GNLPTCGTDK+CIWQFNFREF++  DI+A DSIEL
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           L++ GID  K   +GID  RF ELLMSSGIV            GYDFGYLLKLLTC+NLP
Sbjct: 121 LKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ++Q +FF LI++YFP VYDIKHLM+FCNS HGGLNKLAELLEVERVG+CHQAGSDSLLT+
Sbjct: 181 DSQTDFFKLINVYFPTVYDIKHLMKFCNSLHGGLNKLAELLEVERVGICHQAGSDSLLTS 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 260
           CTFRKL++ FF G   KY+GVLYGLGVE GQ
Sbjct: 241 CTFRKLKENFFVGPLHKYSGVLYGLGVENGQ 271

BLAST of PI0004326 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 8.9e-86
Identity = 156/264 (59.09%), Postives = 194/264 (73.48%), Query Frame = 0

Query: 8   ESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNIN------EYN 67
           ++I IREVWN NLE+E ALI + +D F Y+AMDTEFPG+V + V +  N N      EYN
Sbjct: 12  DTIEIREVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYN 71

Query: 68  YRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELL 127
           Y TL+ NV+ LKLIQLGLT SDE GNLPTCGT+K CIWQFNFREFN+  D++A DSIELL
Sbjct: 72  YDTLKANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIELL 131

Query: 128 RECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLPE 187
           R+  ID +K +E G+D  RF ELLM SG+V            GYDFGYLLKLL+ + LPE
Sbjct: 132 RKSAIDLEKNNECGVDAKRFAELLMGSGVVLNDKIHWVTFHCGYDFGYLLKLLSGKELPE 191

Query: 188 TQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTAC 247
             ++FFD +  +FP+VYDIK+LM FC + +GGL K+AELL V+RVG+ HQAGSDSLLT  
Sbjct: 192 EISDFFDQMEKFFPVVYDIKYLMGFCTNLYGGLEKIAELLGVKRVGISHQAGSDSLLTLR 251

Query: 248 TFRKLRDTFFNGSTQKYAGVLYGL 254
           TF K+++ FF GS  KY+G L+GL
Sbjct: 252 TFIKMKEFFFTGSLLKYSGFLFGL 275

BLAST of PI0004326 vs. ExPASy Swiss-Prot
Match: Q3ZC01 (CCR4-NOT transcription complex subunit 7 OS=Bos taurus OX=9913 GN=CNOT7 PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 3.3e-72
Identity = 138/268 (51.49%), Postives = 182/268 (67.91%), Query Frame = 0

Query: 9   SINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEYNYRTLREN 68
           S  I EVW  NL+EE   IR+++ ++NY+AMDTEFPGVV RP+G F++  +Y Y+ LR N
Sbjct: 9   SQRICEVWACNLDEEMKKIRQVIRKYNYVAMDTEFPGVVARPIGEFRSNADYQYQLLRCN 68

Query: 69  VDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELLRECGIDF 128
           VD LK+IQLGLTF +E G  P  GT     WQFNF+ FN+ +D+YA DSIELL   GI F
Sbjct: 69  VDLLKIIQLGLTFMNEQGEYPP-GTS---TWQFNFK-FNLTEDMYAQDSIELLTTSGIQF 128

Query: 129 QKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLPETQAEFFD 188
           +K  EEGI+   F ELLM+SG+V            GYDFGYL+K+LT  NLPE + +FF+
Sbjct: 129 KKHEEEGIETQYFAELLMTSGVVLCEGVKWLSFHSGYDFGYLIKILTNSNLPEEELDFFE 188

Query: 189 LIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTACTFRKLRD 248
           ++ ++FP++YD+K+LM+ C +  GGL ++AE LE+ER+G  HQAGSDSLLT   F K+R+
Sbjct: 189 ILRLFFPVIYDVKYLMKSCKNLKGGLQEVAEQLELERIGPQHQAGSDSLLTGMAFFKMRE 248

Query: 249 TFFNG--STQKYAGVLYGLGVETGQTTN 263
            FF       KY G LYGLG  +    N
Sbjct: 249 MFFEDHIDDAKYCGHLYGLGSGSSYVQN 271

BLAST of PI0004326 vs. ExPASy TrEMBL
Match: A0A0A0LSM4 (Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_2G382590 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.0e-149
Identity = 260/274 (94.89%), Postives = 262/274 (95.62%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIR+IVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIV            GYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. ExPASy TrEMBL
Match: A0A5A7VC99 (Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002090 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.0e-148
Identity = 259/274 (94.53%), Postives = 261/274 (95.26%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIR+IVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIV            GYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGS QKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSIQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. ExPASy TrEMBL
Match: A0A1S3BD02 (Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103488343 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.0e-148
Identity = 259/274 (94.53%), Postives = 261/274 (95.26%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIR+IVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIV            GYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIXWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGS QKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSIQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. ExPASy TrEMBL
Match: A0A6J1KLE0 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111496296 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.7e-138
Identity = 240/274 (87.59%), Postives = 256/274 (93.43%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESI+IREVWN N+EEEFAL+REIVDQFNYIAMDTEFPGVVVRPVG+FKNIN+Y
Sbjct: 1   MSILPKGESIHIREVWNHNVEEEFALMREIVDQFNYIAMDTEFPGVVVRPVGAFKNINDY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NY+TL++NVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DI+ASDSIEL
Sbjct: 61  NYQTLKDNVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNISNDIFASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LR+CGIDFQK +EEGIDVN FGELLMSSGIV            GYDFGYLLKLLTCR+LP
Sbjct: 121 LRQCGIDFQKNNEEGIDVNHFGELLMSSGIVLNDNVYWVTFHSGYDFGYLLKLLTCRSLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
            TQA FFDLI+MYFPMVYDIKHLM+FCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 GTQAAFFDLINMYFPMVYDIKHLMKFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. ExPASy TrEMBL
Match: A0A6J1GJJ8 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111454439 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.7e-138
Identity = 240/274 (87.59%), Postives = 256/274 (93.43%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESI+IREVWN N+EEEFAL+REIVDQFNYIAMDTEFPGVVVRPVG+FKNIN+Y
Sbjct: 1   MSILPKGESIHIREVWNHNVEEEFALMREIVDQFNYIAMDTEFPGVVVRPVGAFKNINDY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NY+TL++NVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DI+ASDSIEL
Sbjct: 61  NYQTLKDNVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNISNDIFASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LR+CGIDFQK +EEGIDVN FGELLMSSGIV            GYDFGYLLKLLTCR+LP
Sbjct: 121 LRQCGIDFQKNNEEGIDVNHFGELLMSSGIVLNDNVYWVTFHSGYDFGYLLKLLTCRSLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
            TQA FFDLI+MYFPMVYDIKHLM+FCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 GTQAAFFDLINMYFPMVYDIKHLMKFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. NCBI nr
Match: XP_004138706.1 (probable CCR4-associated factor 1 homolog 7 [Cucumis sativus] >KGN62986.1 hypothetical protein Csa_021871 [Cucumis sativus])

HSP 1 Score: 538.1 bits (1385), Expect = 4.2e-149
Identity = 260/274 (94.89%), Postives = 262/274 (95.62%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIR+IVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIV            GYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. NCBI nr
Match: XP_008445257.1 (PREDICTED: LOW QUALITY PROTEIN: probable CCR4-associated factor 1 homolog 7 [Cucumis melo])

HSP 1 Score: 535.8 bits (1379), Expect = 2.1e-148
Identity = 259/274 (94.53%), Postives = 261/274 (95.26%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIR+IVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIV            GYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIXWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGS QKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSIQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. NCBI nr
Match: KAA0064894.1 (putative CCR4-associated factor 1-like protein 7 [Cucumis melo var. makuwa])

HSP 1 Score: 535.8 bits (1379), Expect = 2.1e-148
Identity = 259/274 (94.53%), Postives = 261/274 (95.26%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWNDNLEEEFALIR+IVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNDNLEEEFALIRDIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL
Sbjct: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LRECGIDFQKTHEEGIDVNRFGELLMSSGIV            GYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIVLNDNIFWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGS QKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSIQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. NCBI nr
Match: XP_038886120.1 (probable CCR4-associated factor 1 homolog 7 [Benincasa hispida])

HSP 1 Score: 523.9 bits (1348), Expect = 8.2e-145
Identity = 253/274 (92.34%), Postives = 259/274 (94.53%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESINIREVWN NLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY
Sbjct: 1   MSILPKGESINIREVWNHNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NYRTL+ENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNIC+DIYASDSIEL
Sbjct: 61  NYRTLKENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICEDIYASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LRECGIDF+KT+EEGIDVNRF ELLMSSGIV            GYDFGYLLKLLTCRNLP
Sbjct: 121 LRECGIDFKKTNEEGIDVNRFAELLMSSGIVLNDNIYWVTFHSGYDFGYLLKLLTCRNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           +TQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 QTQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGSTQKYAGVLYGLGVE GQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVEPGQTTN 274

BLAST of PI0004326 vs. NCBI nr
Match: XP_022951690.1 (probable CCR4-associated factor 1 homolog 7 [Cucurbita moschata] >XP_023002466.1 probable CCR4-associated factor 1 homolog 7 [Cucurbita maxima] >KAG6585496.1 putative CCR4-associated factor 1-like 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 500.4 bits (1287), Expect = 9.7e-138
Identity = 240/274 (87.59%), Postives = 256/274 (93.43%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MSILPKGESI+IREVWN N+EEEFAL+REIVDQFNYIAMDTEFPGVVVRPVG+FKNIN+Y
Sbjct: 1   MSILPKGESIHIREVWNHNVEEEFALMREIVDQFNYIAMDTEFPGVVVRPVGAFKNINDY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           NY+TL++NVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DI+ASDSIEL
Sbjct: 61  NYQTLKDNVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNISNDIFASDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           LR+CGIDFQK +EEGIDVN FGELLMSSGIV            GYDFGYLLKLLTCR+LP
Sbjct: 121 LRQCGIDFQKNNEEGIDVNHFGELLMSSGIVLNDNVYWVTFHSGYDFGYLLKLLTCRSLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
            TQA FFDLI+MYFPMVYDIKHLM+FCNSFHGGLNKLAELLEVER+GVCHQAGSDSLLTA
Sbjct: 181 GTQAAFFDLINMYFPMVYDIKHLMKFCNSFHGGLNKLAELLEVERIGVCHQAGSDSLLTA 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 263
           CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN
Sbjct: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQTTN 274

BLAST of PI0004326 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 4.7e-114
Identity = 196/272 (72.06%), Postives = 225/272 (82.72%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNLE E ALIRE+VD F ++AMDTEFPG+V RPVG+FK   EY
Sbjct: 1   MSLFLKDDSIQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRPVGTFKTNTEY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTD-KFCIWQFNFREFNICDDIYASDSIE 120
           +Y TL+ NV+ LK+IQLGLTFSDE GNLPTCGTD K+CIWQFNFREF++  DIYA+DSIE
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE 120

Query: 121 LLRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNL 180
           LLR+ GIDF K +E GID  RF ELLMSSGIV            GYDFGYLLKLLTC+NL
Sbjct: 121 LLRQSGIDFVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 180

Query: 181 PETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLT 240
           PETQ  FF++I +YFP VYDIKHLM+FCNS HGGLNKLAELL+VERVG+CHQAGSDSLLT
Sbjct: 181 PETQTGFFEMISVYFPRVYDIKHLMKFCNSLHGGLNKLAELLDVERVGICHQAGSDSLLT 240

Query: 241 ACTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 260
           +CTFRKL++ FF GS +KY+GVLYGLGVE GQ
Sbjct: 241 SCTFRKLQENFFIGSMEKYSGVLYGLGVENGQ 272

BLAST of PI0004326 vs. TAIR 10
Match: AT5G10960.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 405.6 bits (1041), Expect = 3.0e-113
Identity = 196/268 (73.13%), Postives = 226/268 (84.33%), Query Frame = 0

Query: 6   KGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEYNYRTL 65
           K +SI IREVW+ NL EEFALIREIVD+F+YIAMDTEFPGVV++PV +FK  N+ NYRTL
Sbjct: 6   KEDSIMIREVWDYNLVEEFALIREIVDKFSYIAMDTEFPGVVLKPVATFKYNNDLNYRTL 65

Query: 66  RENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIELLRECG 125
           +ENVD LKLIQ+GLTFSDENGNLPTCGTDKFCIWQFNFREFNI +DIYAS+SIELLR+CG
Sbjct: 66  KENVDLLKLIQVGLTFSDENGNLPTCGTDKFCIWQFNFREFNIGEDIYASESIELLRQCG 125

Query: 126 IDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLPETQAE 185
           IDF+K  E+GIDV RFGEL+MSSGIV            GYDFGYL+KLLTC+ LP  QA+
Sbjct: 126 IDFKKNIEKGIDVVRFGELMMSSGIVLNDAISWVTFHGGYDFGYLVKLLTCKELPLKQAD 185

Query: 186 FFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTACTFRK 245
           FF L+++YFP VYDIKHLM FCN   GGLN+LAEL+ VERVG+CHQAGSDSLLT  +FRK
Sbjct: 186 FFKLLYVYFPTVYDIKHLMTFCNGLFGGLNRLAELMGVERVGICHQAGSDSLLTLGSFRK 245

Query: 246 LRDTFFNGSTQKYAGVLYGLGVETGQTT 262
           L++ +F GST+KY GVLYGLGVE G TT
Sbjct: 246 LKERYFPGSTEKYTGVLYGLGVEDGTTT 273

BLAST of PI0004326 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 9.7e-112
Identity = 189/271 (69.74%), Postives = 223/271 (82.29%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNL+EE  LIR++VD F Y+AMDTEFPG+VVRPVG+FK+  +Y
Sbjct: 1   MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           +Y TL+ NV+ LK+IQLGLTFS+E GNLPTCGTDK+CIWQFNFREF++  DI+A DSIEL
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           L++ GID  K   +GID  RF ELLMSSGIV            GYDFGYLLKLLTC+NLP
Sbjct: 121 LKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ++Q +FF LI++YFP VYDIKHLM+FCNS HGGLNKLAELLEVERVG+CHQAGSDSLLT+
Sbjct: 181 DSQTDFFKLINVYFPTVYDIKHLMKFCNSLHGGLNKLAELLEVERVGICHQAGSDSLLTS 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 260
           CTFRKL++ FF G   KY+GVLYGLGVE GQ
Sbjct: 241 CTFRKLKENFFVGPLHKYSGVLYGLGVENGQ 271

BLAST of PI0004326 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 9.7e-112
Identity = 189/271 (69.74%), Postives = 223/271 (82.29%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNL+EE  LIR++VD F Y+AMDTEFPG+VVRPVG+FK+  +Y
Sbjct: 1   MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           +Y TL+ NV+ LK+IQLGLTFS+E GNLPTCGTDK+CIWQFNFREF++  DI+A DSIEL
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           L++ GID  K   +GID  RF ELLMSSGIV            GYDFGYLLKLLTC+NLP
Sbjct: 121 LKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ++Q +FF LI++YFP VYDIKHLM+FCNS HGGLNKLAELLEVERVG+CHQAGSDSLLT+
Sbjct: 181 DSQTDFFKLINVYFPTVYDIKHLMKFCNSLHGGLNKLAELLEVERVGICHQAGSDSLLTS 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVETGQ 260
           CTFRKL++ FF G   KY+GVLYGLGVE GQ
Sbjct: 241 CTFRKLKENFFVGPLHKYSGVLYGLGVENGQ 271

BLAST of PI0004326 vs. TAIR 10
Match: AT1G80780.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 1.4e-110
Identity = 187/268 (69.78%), Postives = 221/268 (82.46%), Query Frame = 0

Query: 1   MSILPKGESINIREVWNDNLEEEFALIREIVDQFNYIAMDTEFPGVVVRPVGSFKNINEY 60
           MS+  K +SI IREVWNDNL+EE  LIR++VD F Y+AMDTEFPG+VVRPVG+FK+  +Y
Sbjct: 1   MSLFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY 60

Query: 61  NYRTLRENVDTLKLIQLGLTFSDENGNLPTCGTDKFCIWQFNFREFNICDDIYASDSIEL 120
           +Y TL+ NV+ LK+IQLGLTFS+E GNLPTCGTDK+CIWQFNFREF++  DI+A DSIEL
Sbjct: 61  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIEL 120

Query: 121 LRECGIDFQKTHEEGIDVNRFGELLMSSGIV------------GYDFGYLLKLLTCRNLP 180
           L++ GID  K   +GID  RF ELLMSSGIV            GYDFGYLLKLLTC+NLP
Sbjct: 121 LKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLP 180

Query: 181 ETQAEFFDLIHMYFPMVYDIKHLMRFCNSFHGGLNKLAELLEVERVGVCHQAGSDSLLTA 240
           ++Q +FF LI++YFP VYDIKHLM+FCNS HGGLNKLAELLEVERVG+CHQAGSDSLLT+
Sbjct: 181 DSQTDFFKLINVYFPTVYDIKHLMKFCNSLHGGLNKLAELLEVERVGICHQAGSDSLLTS 240

Query: 241 CTFRKLRDTFFNGSTQKYAGVLYGLGVE 257
           CTFRKL++ FF G   KY+GVLYGLGVE
Sbjct: 241 CTFRKLKENFFVGPLHKYSGVLYGLGVE 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKZ26.6e-11372.06Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LEU44.3e-11273.13Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SAI21.4e-11069.74Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9S9P28.9e-8659.09Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q3ZC013.3e-7251.49CCR4-NOT transcription complex subunit 7 OS=Bos taurus OX=9913 GN=CNOT7 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LSM42.0e-14994.89Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_2G382590 PE=3 SV... [more]
A0A5A7VC991.0e-14894.53Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3BD021.0e-14894.53Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103488343 PE=3 SV=1[more]
A0A6J1KLE04.7e-13887.59Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111496296 PE=3 S... [more]
A0A6J1GJJ84.7e-13887.59Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111454439 PE=3... [more]
Match NameE-valueIdentityDescription
XP_004138706.14.2e-14994.89probable CCR4-associated factor 1 homolog 7 [Cucumis sativus] >KGN62986.1 hypoth... [more]
XP_008445257.12.1e-14894.53PREDICTED: LOW QUALITY PROTEIN: probable CCR4-associated factor 1 homolog 7 [Cuc... [more]
KAA0064894.12.1e-14894.53putative CCR4-associated factor 1-like protein 7 [Cucumis melo var. makuwa][more]
XP_038886120.18.2e-14592.34probable CCR4-associated factor 1 homolog 7 [Benincasa hispida][more]
XP_022951690.19.7e-13887.59probable CCR4-associated factor 1 homolog 7 [Cucurbita moschata] >XP_023002466.1... [more]
Match NameE-valueIdentityDescription
AT2G32070.14.7e-11472.06Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G10960.13.0e-11373.13Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.19.7e-11269.74Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.29.7e-11269.74Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.31.4e-11069.78Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 153..231
e-value: 4.4E-6
score: 26.2
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 15..136
e-value: 1.2E-12
score: 47.8
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..256
e-value: 7.3E-89
score: 299.5
NoneNo IPR availablePANTHERPTHR10797:SF68CCR4-ASSOCIATED FACTOR 1 HOMOLOG 10-RELATEDcoord: 1..262
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 1..262
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 12..253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0004326.1PI0004326.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043928 exonucleolytic catabolism of deadenylated mRNA
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030015 CCR4-NOT core complex
cellular_component GO:0005634 nucleus
cellular_component GO:0000932 P-body
cellular_component GO:0030014 CCR4-NOT complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity