PI0004255 (gene) Melon (PI 482460) v1

Overview


Name	PI0004255
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Pectinesterase
Location	chr03: 1911629 .. 1913760 (-)
RNA-Seq Expression	PI0004255
Synteny	PI0004255

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCGTCGGAGTTTTTTAGTTGCGATTAGTGTTCTTGCCGCGGTCTTAGTGGCAGAGGTTACAGCGGCGGCGGCAACTACGGCGGTTTTGATAACTGTGGATCAATCTGGGAAGGGGAATTTTACGAAGATTCAAGAGGCGATTGATGCAGTGCCGGAAAATAACAGGGAGGAAGTGTTTATTGCGGTGAAGGCGGGGATTTATAGAGAGAAGGTGGTGGTTCCGGCGAATAAACCGTTCATTACTATAAGTGGAAGAAGAGCGGTGGATACGATCATCAGTTGGAATGATAGCAAAAATACTTATAATTCTGCAACTCTTGCAGTTTTGGCCTCTGATTTCGTTGGACGATACCTCACAATTCAGGTTTCTAATTTCAACAGAAAAAAAAATATATATTTATCTTCGACTTTATTTCGGTTATCTTATATATGAAAACAATAACAACAACAACAACATGACATTTCTAAAATTTGAAAATCTTACTATTTTTGCGGATGAGTTTTGAGTTTTCTGTTGGTTTGGTTGTTTTTGAATTTTACGTTTTCATTTTTTATTTAAGGGAAAAAAGGGGAAATAGTTTTTAATTTAAATGAATTATAACTAAGTTATTATTTTTTAAATCTTTCTATCACCATTAAATTTAAAATTTTCGCATCATAATTATTTTTAAGTACTTGGTTGAAATTTAATGTCATATACTGAAAATAATAAGCATGGTAAGAAATGGAAAAAATTGTATACAATTTGAAATTTAGGAACGAAATGAATGTTGAACTCAAAATCTCACGATAAAAGAAATCTTATTTTGAAATTTAAAAACAAAACGAAAATGTAATTAAAATTTTATGAGTATGATAAGAGCACCAACGAATTTAGTATAGACTCAGAGAATCTTAAATCTACTTTCAACTTTATGCTCTATATATAATATATATAAACTAAAACAATATCAAACTTTTTAACCTAATCTAAACTCATATTATTTTCTAAAAATGTATGCCCTCTTTGATTTTTTTAAAGTACTTTTCTTGATTATCAACCCTCTCAATCTCAGTATGCGAAAAGGTGAAGAAGACATCTCTAAGTCTCTATATAACTTAAAACTTTTATGAATTTTTTAAAAAAATTTATTTTCAATTAGTTAAAATATGTGAATTCTGACGAAGTATAAATACTTTTAACAACTAAAGTCATGTTCTGATCATTGACATATACATAACTATTAATAAAAACAAAAACAAAAAATACAAAACTAAACTTAAACTTAAGTTTTATTTACTGATTAAAATAAAGAAAAAGTTTGATTGACTTATTATTTAATTGATTGGGGTCGAAGAATGGATACGGGCCGGGTGCACAGGCAGTGGCCTTACGAGTATCTGGAGATAGAGTATCATTTACTGCATGTAGATTTTTAGGCCATCAAGACACTCTTCTAGATGATATTGGCCGTCATTACTACAAAAGTTGTTACATCGAAGGAGCCACCGATTTCATATGTGGAAACTCAGCTTCACTGTTCGAGGTTTGAATCACATTTAAATCAATACACATATATAAATGGGCCTATTGGGCTTGGCATAATGGGCTATTTAATGTTGGGCTTTTGCAGAATTGCCATCTACGGTCAGTTTCGGACGCGGTCGGGACGATCACAGCCCAACGGAGAGAATCGCCGTCGGAGAATACAGGGTTCGTATTCATGGGGTGTAAGATCACCGGAATAAACTCGGCGGTTTTAGGAAGGCCGTGGGGAGCATTCTCTAGAGTGGTCTTTGGATTCAGTTTCATGTCGGACGTTATTCTCCCTGAGGGTTGGGATAATTGGCAAGACCCATCCAAACAAAGGTTTATTATTTTATTTATTTTAATTAATTAACTAAACTATTTTCTACTTTTCTAAATCACAACTTAATTAATTAAATGTATTTGGCAGTACGGTTTACTACGGGCAATACAAATGTTATGGAAAAGGAGCAAATCCTTCGAGAAGGGTACCGTGGTCATTTACTAACATGACTGCCCAAGATGCTGCACCATTTTTCACCAAATCCTTCATTGGTGCTGCTGATTGGCTGAGGCCATTGCCAAATCATTTCAAGAGAGCCTTCTCTTCACAGCCCTAA

mRNA sequence

ATGTCTCGTCGGAGTTTTTTAGTTGCGATTAGTGTTCTTGCCGCGGTCTTAGTGGCAGAGGTTACAGCGGCGGCGGCAACTACGGCGGTTTTGATAACTGTGGATCAATCTGGGAAGGGGAATTTTACGAAGATTCAAGAGGCGATTGATGCAGTGCCGGAAAATAACAGGGAGGAAGTAGAGAAGGTGGTGGTTCCGGCGAATAAACCGTTCATTACTATAAGTGGAAGAAGAGCGGTGGATACGATCATCAGTTGGAATGATAGCAAAAATACTTATAATTCTGCAACTCTTGCAGTTTTGGCCTCTGATTTCGTTGGACGATACCTCACAATTCAGAATGGATACGGGCCGGGTGCACAGGCAGTGGCCTTACGAGTATCTGGAGATAGAGTATCATTTACTGCATGTAGATTTTTAGGCCATCAAGACACTCTTCTAGATGATATTGGCCGTCATTACTACAAAAGTTGTTACATCGAAGGAGCCACCGATTTCATATGTGGAAACTCAGCTTCACTGTTCGAGAATTGCCATCTACGGTCAGTTTCGGACGCGGTCGGGACGATCACAGCCCAACGGAGAGAATCGCCGTCGGAGAATACAGGGTTCGTATTCATGGGGTGTAAGATCACCGGAATAAACTCGGCGGTTTTAGGAAGGCCGTGGGGAGCATTCTCTAGAGTGGTCTTTGGATTCAGTTTCATGTCGGACGTTATTCTCCCTGAGGGTTGGGATAATTGGCAAGACCCATCCAAACAAAGTACGGTTTACTACGGGCAATACAAATGTTATGGAAAAGGAGCAAATCCTTCGAGAAGGGTACCGTGGTCATTTACTAACATGACTGCCCAAGATGCTGCACCATTTTTCACCAAATCCTTCATTGGTGCTGCTGATTGGCTGAGGCCATTGCCAAATCATTTCAAGAGAGCCTTCTCTTCACAGCCCTAA

Coding sequence (CDS)

ATGTCTCGTCGGAGTTTTTTAGTTGCGATTAGTGTTCTTGCCGCGGTCTTAGTGGCAGAGGTTACAGCGGCGGCGGCAACTACGGCGGTTTTGATAACTGTGGATCAATCTGGGAAGGGGAATTTTACGAAGATTCAAGAGGCGATTGATGCAGTGCCGGAAAATAACAGGGAGGAAGTAGAGAAGGTGGTGGTTCCGGCGAATAAACCGTTCATTACTATAAGTGGAAGAAGAGCGGTGGATACGATCATCAGTTGGAATGATAGCAAAAATACTTATAATTCTGCAACTCTTGCAGTTTTGGCCTCTGATTTCGTTGGACGATACCTCACAATTCAGAATGGATACGGGCCGGGTGCACAGGCAGTGGCCTTACGAGTATCTGGAGATAGAGTATCATTTACTGCATGTAGATTTTTAGGCCATCAAGACACTCTTCTAGATGATATTGGCCGTCATTACTACAAAAGTTGTTACATCGAAGGAGCCACCGATTTCATATGTGGAAACTCAGCTTCACTGTTCGAGAATTGCCATCTACGGTCAGTTTCGGACGCGGTCGGGACGATCACAGCCCAACGGAGAGAATCGCCGTCGGAGAATACAGGGTTCGTATTCATGGGGTGTAAGATCACCGGAATAAACTCGGCGGTTTTAGGAAGGCCGTGGGGAGCATTCTCTAGAGTGGTCTTTGGATTCAGTTTCATGTCGGACGTTATTCTCCCTGAGGGTTGGGATAATTGGCAAGACCCATCCAAACAAAGTACGGTTTACTACGGGCAATACAAATGTTATGGAAAAGGAGCAAATCCTTCGAGAAGGGTACCGTGGTCATTTACTAACATGACTGCCCAAGATGCTGCACCATTTTTCACCAAATCCTTCATTGGTGCTGCTGATTGGCTGAGGCCATTGCCAAATCATTTCAAGAGAGCCTTCTCTTCACAGCCCTAA

Protein sequence

MSRRSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEVEKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYLTIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNSASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFFTKSFIGAADWLRPLPNHFKRAFSSQP

Homology

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BLAST of PI0004255 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-93
Identity = 165/324 (50.93%), Postives = 233/324 (71.91%), Query Frame = 0


Query: 7   LVAISVLAAVLVAEVTAAAA------TTAVLITVDQSGKGNFTKIQEAIDAVPEN-NREE 66
           ++ I +L+++  + + ++++      +TA+LI VDQSGKG+F+KIQEAI+++P N N  +
Sbjct: 20  IINIFILSSITSSSMASSSSPSSIDFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQ 79

Query: 67  V-----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGR 126
           +           EKVV+PA KP+IT+SG +A +T + W+D ++   S TL + ASDFV R
Sbjct: 80  LYFIWVKPGIYREKVVIPAEKPYITLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCR 139

Query: 127 YLTIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 186
           +LTIQN +G   +AVALRV+ D+ +F  C    +QDTLLDD G HY+K+CYIEGATDFIC
Sbjct: 140 FLTIQNKFGTAGRAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFIC 199

Query: 187 GNSASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 246
           G+++SL+E CHL S+S   G+ITAQ R S +E +GF F+GCK+TG  S  LGRPWGA+SR
Sbjct: 200 GSASSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSR 259

Query: 247 VVFGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAA 306
           VVF +SF S+V+ P+GW+ W D +K++TVYYG+YKCYG GA+  +RV WS   ++ ++A 
Sbjct: 260 VVFAYSFFSNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWS-KQLSDEEAT 319

Query: 307 PFFTKSFIGAADWLRPLPNHFKRA 313
            F +K FIG  DWLRP P+HFK A
Sbjct: 320 VFLSKDFIGGKDWLRPAPSHFKNA 342

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BLAST of PI0004255 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-73
Identity = 142/294 (48.30%), Postives = 187/294 (63.61%), Query Frame = 0


Query: 36  QSGKGNFTKIQEAIDAVPENNREEV----------EKVVVPANKPFITISGRRAVDTIIS 95
           +S KG+FTKIQ+AID++P  N   V          EKV +P  K FITI G  A  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 96  WNDS----------KNTYNSATLAVLASDFVGRYLTIQNGYG---PGA---QAVALRVSG 155
           W D+            TYNSA+ AV +  FV + +T +N      PGA   QAVALRVS 
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 156 DRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNSASLFENCHLRSVSDAVGT 215
           D  +F  CR LG QDTL D +GRHYYK CYIEG+ DFI GN+ SL+E CH+ +++D +G 
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 216 ITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFSFMSDVILPEGWDNWQ 275
           +TAQ R S  E+TGF F+ CK+TG     LGR WG FSRVVF +++M ++ILP GW NW 
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 276 DPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFFTKSFIGAADWLR 304
           DPS++ TV+YGQYKC G GAN   RV W+   +T ++A PF + +FI  ++W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWA-RELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of PI0004255 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.6e-63
Identity = 130/301 (43.19%), Postives = 183/301 (60.80%), Query Frame = 0


Query: 28  TAVLITVDQSGKGNFTKIQEAIDAVPE----------NNREEVEKVVVPANKPFITISGR 87
           T++++TVD  G GNF+ +Q AID VP+          N+    EKV V  NK  + I GR
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 88  RAVDTIISWNDSK----NTYNSATLAVLASDFVGRYLTIQNGY---GPG---AQAVALRV 147
              +T I WND+     NT +S +  V A++F    ++ +N      PG   AQAVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 148 SGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNSASLFENCHLRSV---- 207
            GD+ +F  C F G QDTLLDD GRH++K C+I+G+ DFI GN  SL+++C + S+    
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 208 -SDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFSFMSDVILP 267
            S   G+ITAQ R+S  E +GF F+ CKI G    +LGR WGA++ VVF  ++MS +I P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 268 EGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFFTKSFIGAADWL 304
           EGW+NW D +K+ TV +G++KCYG GA+   RV +    +T  +A+ F   SFI   +WL
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFG-KQLTDSEASSFIDVSFIDGDEWL 386

[Close]

BLAST of PI0004255 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.9e-61
Identity = 118/301 (39.20%), Postives = 170/301 (56.48%), Query Frame = 0


Query: 31  LITVDQSGKGNFTKIQEAIDAVPENNREEV----------EKVVVPANKPFITISGRRAV 90
           +ITV  +G   F  +Q+A+D++P+NN + +          EKVVVPA KP+IT  G    
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 91  DTIISWNDSKN----------TYNSATLAVLASDFVGRYLTIQNGYG------PGAQAVA 150
            T I W+D  +          TY +A++ V A+ F  R ++  N          G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 151 LRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNSASLFENCHLRSVS 210
            R+SGD+  F+ C F G QDTL DD GRHY+K CYIEG+ DFI GN  S++++C L S++
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 211 DAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFSFMSDVILPEG 270
              G+I A  R  P E TGF F+GC++TG     +GR  G +SR+V+ +++   ++   G
Sbjct: 240 SRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGG 299

Query: 271 WDNWQDPS-KQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFFTKSFIGAADWLR 305
           WD+W   S K  T ++G Y CYG GA  +R V W+   +  + A PF  KSF+    W+ 
Sbjct: 300 WDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWA-RALDYESAHPFIAKSFVNGRHWIA 359

[Close]

BLAST of PI0004255 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.3e-59
Identity = 126/327 (38.53%), Postives = 181/327 (55.35%), Query Frame = 0


Query: 4   RSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV--- 63
           RSF +  +       A   A A     +I VD++G G+   +Q A+D VP++N + V   
Sbjct: 54  RSFSIKENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIF 113

Query: 64  -------EKVVVPANKPFITISGRR--AVDTIISWNDSKN----------TYNSATLAVL 123
                  EKV+VP +KP+I+  G    A DT+ISW+D  +          TY +A++++ 
Sbjct: 114 ILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIE 173

Query: 124 ASDFVGRYLTIQN-----GYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYK 183
           +  F    +T +N         G QAVALR+ GD+  F   R LG QDTL DD G HY+ 
Sbjct: 174 SDFFCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFY 233

Query: 184 SCYIEGATDFICGNSASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINS 243
            CYI+G  DFI GN+ SL+++C + S +   G I A  R+S +E+TGF F+ C I+G   
Sbjct: 234 QCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQ 293

Query: 244 AVLGRPWGAFSRVVFGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVP 303
             LGR WG +SR V+   F++D+I P GW +W+ P +Q  V +G+Y C G+GA    RVP
Sbjct: 294 IYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVP 353

[Close]

BLAST of PI0004255 vs. ExPASy TrEMBL
Match: A0A0A0LPB4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G361760 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 9.0e-168
Identity = 300/327 (91.74%), Postives = 309/327 (94.50%), Query Frame = 0


Query: 1   MSRRSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV 60
           MSR SFL+AISV+AAVLVAEVTAAA TTAVLITVDQSGKGNFTKIQ+AIDAVP NN+EEV
Sbjct: 1   MSRPSFLLAISVIAAVLVAEVTAAAVTTAVLITVDQSGKGNFTKIQQAIDAVPINNKEEV 60

Query: 61  ----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL 120
                     EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL
Sbjct: 61  FISVKAGIYREKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL 120

Query: 121 TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN 180
           TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGN
Sbjct: 121 TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGN 180

Query: 181 SASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV 240
           +ASLFENCHLRSVS+ VGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV
Sbjct: 181 AASLFENCHLRSVSEDVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV 240

Query: 241 FGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPF 300
           FGF+FMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGAN SRRV WSFTNMTAQDAAPF
Sbjct: 241 FGFTFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANTSRRVSWSFTNMTAQDAAPF 300

Query: 301 FTKSFIGAADWLRPLPNHFKRAFSSQP 318
           FTKSFIGAADWLRP+PN FKRAFSS P
Sbjct: 301 FTKSFIGAADWLRPVPNRFKRAFSSHP 327

[Close]

BLAST of PI0004255 vs. ExPASy TrEMBL
Match: A0A1S3BBW3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103487980 PE=4 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.7e-166
Identity = 299/326 (91.72%), Postives = 307/326 (94.17%), Query Frame = 0


Query: 1   MSRRSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV 60
           MSRRSFLV +SV+AAVLVAEVT AA TTAVLITVDQSGKGNFTKIQ+AIDAVPENNREEV
Sbjct: 1   MSRRSFLVVVSVIAAVLVAEVT-AAETTAVLITVDQSGKGNFTKIQQAIDAVPENNREEV 60

Query: 61  ----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL 120
                     EKVVVPANKPFITISGRRA DTIISWNDSKNTYNSATLAVLASDFVGRYL
Sbjct: 61  FIVVKAGIYREKVVVPANKPFITISGRRAADTIISWNDSKNTYNSATLAVLASDFVGRYL 120

Query: 121 TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN 180
           TIQN YG GAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGN
Sbjct: 121 TIQNEYGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGN 180

Query: 181 SASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV 240
           +ASLFENCHLRSVSD VGTITAQRRESPSENTGFVF+GCKITGINSAVLGRPWGAFSRVV
Sbjct: 181 AASLFENCHLRSVSDDVGTITAQRRESPSENTGFVFLGCKITGINSAVLGRPWGAFSRVV 240

Query: 241 FGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPF 300
           FGF+FMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRV WSFTNMTAQDAAPF
Sbjct: 241 FGFTFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVSWSFTNMTAQDAAPF 300

Query: 301 FTKSFIGAADWLRPLPNHFKRAFSSQ 317
           FTKSFIGAADWLRPLP HFKRAFSS+
Sbjct: 301 FTKSFIGAADWLRPLPKHFKRAFSSR 325

[Close]

BLAST of PI0004255 vs. ExPASy TrEMBL
Match: A0A6J1GIB5 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454415 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 7.9e-156
Identity = 274/326 (84.05%), Postives = 295/326 (90.49%), Query Frame = 0


Query: 2   SRRSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV- 61
           SR  F    +++  VLVAE TAA A TA+LITVDQSG+GNFTKIQ AIDAVPENN EEV 
Sbjct: 4   SRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLEEVF 63

Query: 62  ---------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYLT 121
                    EKV VPANKP+ITISGRR+ DTII+WNDS+NTYNSATLAVLASDFVGRYLT
Sbjct: 64  IAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLT 123

Query: 122 IQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNS 181
           IQNG+G GAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYY+SCYIEGATDFICGN 
Sbjct: 124 IQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFICGNG 183

Query: 182 ASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 241
           ASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF
Sbjct: 184 ASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 243

Query: 242 GFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFF 301
           GF++MSDVILPEGWDNWQDPSKQSTVYYGQY+CYGKGANPSRRVPWSFTNMT Q+AAPFF
Sbjct: 244 GFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAAPFF 303

Query: 302 TKSFIGAADWLRPLPNHFKRAFSSQP 318
           +K+FIGAA WLRPLP +FKRAF+S+P
Sbjct: 304 SKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of PI0004255 vs. ExPASy TrEMBL
Match: A0A6J1KQF0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496269 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.3e-155
Identity = 273/326 (83.74%), Postives = 295/326 (90.49%), Query Frame = 0


Query: 2   SRRSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV- 61
           SR  F    +++  VLVAE TAA A TA+LITVDQSG+GNFTKIQ AIDAVP+NN EEV 
Sbjct: 4   SRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVF 63

Query: 62  ---------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYLT 121
                    EKV VPANKP+ITISGRR+ DTII+WNDS+NTYNSATLAVLASDFVGRYLT
Sbjct: 64  IAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLT 123

Query: 122 IQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNS 181
           IQNG+G GAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN 
Sbjct: 124 IQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNG 183

Query: 182 ASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 241
           ASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF
Sbjct: 184 ASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 243

Query: 242 GFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFF 301
           GF++MSDVILPEGWDNWQDPSKQSTVYYGQY+CYGKGANPSRRVPWSF+NMT Q+AAPFF
Sbjct: 244 GFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFF 303

Query: 302 TKSFIGAADWLRPLPNHFKRAFSSQP 318
           +K+FIGAA WLRPLP +FKRAF+S+P
Sbjct: 304 SKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of PI0004255 vs. ExPASy TrEMBL
Match: A0A6J1CQY5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013943 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.3e-154
Identity = 274/333 (82.28%), Postives = 297/333 (89.19%), Query Frame = 0


Query: 1   MSRRS------FLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPE 60
           MSRRS       +  ISV  AV VAE+T AAAT A+LITVDQSG+GNFTKIQ+AIDAVP 
Sbjct: 1   MSRRSDFPRITTIALISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPV 60

Query: 61  NNREEV----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASD 120
           NN E+V          EKV+VPANKPFITISGRRAVDTI++WNDS+ TYNSATLAVLASD
Sbjct: 61  NNVEQVFIAVKPGIYREKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASD 120

Query: 121 FVGRYLTIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGAT 180
           FVGRYLTIQNG+GPGAQAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYYKSCYIEGAT
Sbjct: 121 FVGRYLTIQNGFGPGAQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGAT 180

Query: 181 DFICGNSASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWG 240
           DFICGN+ASLFENCHL SVSDAVGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWG
Sbjct: 181 DFICGNAASLFENCHLHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWG 240

Query: 241 AFSRVVFGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTA 300
           A+SRVVFG+++MSD+ILP+GWD+WQDPSK STVYYGQY+CYGKGANPSRRVPWS TNMT+
Sbjct: 241 AYSRVVFGYTYMSDLILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTS 300

Query: 301 QDAAPFFTKSFIGAADWLRPLPNHFKRAFSSQP 318
           Q+AAPFF KSFIGAADWLRPLP  FKRAFSS+P
Sbjct: 301 QEAAPFFNKSFIGAADWLRPLPTTFKRAFSSKP 333

[Close]

BLAST of PI0004255 vs. NCBI nr
Match: XP_004152775.1 (putative pectinesterase 11 [Cucumis sativus] >KGN62612.1 hypothetical protein Csa_021853 [Cucumis sativus])

HSP 1 Score: 599.4 bits (1544), Expect = 1.9e-167
Identity = 300/327 (91.74%), Postives = 309/327 (94.50%), Query Frame = 0


Query: 1   MSRRSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV 60
           MSR SFL+AISV+AAVLVAEVTAAA TTAVLITVDQSGKGNFTKIQ+AIDAVP NN+EEV
Sbjct: 1   MSRPSFLLAISVIAAVLVAEVTAAAVTTAVLITVDQSGKGNFTKIQQAIDAVPINNKEEV 60

Query: 61  ----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL 120
                     EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL
Sbjct: 61  FISVKAGIYREKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL 120

Query: 121 TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN 180
           TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGN
Sbjct: 121 TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGN 180

Query: 181 SASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV 240
           +ASLFENCHLRSVS+ VGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV
Sbjct: 181 AASLFENCHLRSVSEDVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV 240

Query: 241 FGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPF 300
           FGF+FMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGAN SRRV WSFTNMTAQDAAPF
Sbjct: 241 FGFTFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANTSRRVSWSFTNMTAQDAAPF 300

Query: 301 FTKSFIGAADWLRPLPNHFKRAFSSQP 318
           FTKSFIGAADWLRP+PN FKRAFSS P
Sbjct: 301 FTKSFIGAADWLRPVPNRFKRAFSSHP 327

[Close]

BLAST of PI0004255 vs. NCBI nr
Match: XP_008444730.1 (PREDICTED: putative pectinesterase 11 [Cucumis melo])

HSP 1 Score: 595.1 bits (1533), Expect = 3.5e-166
Identity = 299/326 (91.72%), Postives = 307/326 (94.17%), Query Frame = 0


Query: 1   MSRRSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV 60
           MSRRSFLV +SV+AAVLVAEVT AA TTAVLITVDQSGKGNFTKIQ+AIDAVPENNREEV
Sbjct: 1   MSRRSFLVVVSVIAAVLVAEVT-AAETTAVLITVDQSGKGNFTKIQQAIDAVPENNREEV 60

Query: 61  ----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL 120
                     EKVVVPANKPFITISGRRA DTIISWNDSKNTYNSATLAVLASDFVGRYL
Sbjct: 61  FIVVKAGIYREKVVVPANKPFITISGRRAADTIISWNDSKNTYNSATLAVLASDFVGRYL 120

Query: 121 TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN 180
           TIQN YG GAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGN
Sbjct: 121 TIQNEYGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGN 180

Query: 181 SASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV 240
           +ASLFENCHLRSVSD VGTITAQRRESPSENTGFVF+GCKITGINSAVLGRPWGAFSRVV
Sbjct: 181 AASLFENCHLRSVSDDVGTITAQRRESPSENTGFVFLGCKITGINSAVLGRPWGAFSRVV 240

Query: 241 FGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPF 300
           FGF+FMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRV WSFTNMTAQDAAPF
Sbjct: 241 FGFTFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVSWSFTNMTAQDAAPF 300

Query: 301 FTKSFIGAADWLRPLPNHFKRAFSSQ 317
           FTKSFIGAADWLRPLP HFKRAFSS+
Sbjct: 301 FTKSFIGAADWLRPLPKHFKRAFSSR 325

[Close]

BLAST of PI0004255 vs. NCBI nr
Match: XP_038884203.1 (putative pectinesterase 11 [Benincasa hispida])

HSP 1 Score: 589.7 bits (1519), Expect = 1.5e-164
Identity = 294/329 (89.36%), Postives = 310/329 (94.22%), Query Frame = 0


Query: 1   MSRRSFLVAISVLAAVLVAEVTAAAAT--TAVLITVDQSGKGNFTKIQEAIDAVPENNRE 60
           MS RSFLVAISVLAAVL+AEVTAAAA   TA+LITVDQSGKGNFTKIQEAIDAVPENNRE
Sbjct: 1   MSCRSFLVAISVLAAVLLAEVTAAAAAAKTAILITVDQSGKGNFTKIQEAIDAVPENNRE 60

Query: 61  EV----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGR 120
           EV          EK+VVPANKP+ITISGR A DTIISWNDSKNTYNSATLAVLASDFVGR
Sbjct: 61  EVFIAVKAGIYREKLVVPANKPYITISGRTAADTIISWNDSKNTYNSATLAVLASDFVGR 120

Query: 121 YLTIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180
           YLTIQN +GPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYI+GATDFIC
Sbjct: 121 YLTIQNEFGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFIC 180

Query: 181 GNSASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240
           GN+ASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR
Sbjct: 181 GNAASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240

Query: 241 VVFGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAA 300
           VVFGF++MSD+ILPEGWD+WQDPSKQSTV+YGQY+CYGKGANPSRRVPWSFTNMT ++AA
Sbjct: 241 VVFGFTYMSDLILPEGWDDWQDPSKQSTVFYGQYRCYGKGANPSRRVPWSFTNMTTEEAA 300

Query: 301 PFFTKSFIGAADWLRPLPNHFKRAFSSQP 318
           PFFTKSFIGAADWLRPLP HFKRAFSS+P
Sbjct: 301 PFFTKSFIGAADWLRPLPTHFKRAFSSKP 329

[Close]

BLAST of PI0004255 vs. NCBI nr
Match: XP_023536774.1 (putative pectinesterase 11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.5 bits (1443), Expect = 9.6e-156
Identity = 277/327 (84.71%), Postives = 297/327 (90.83%), Query Frame = 0


Query: 2   SRRSFLVA-ISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV 61
           S R FL A  +++  VLVAE TAA A TA+LITVDQSG+GNFTKIQ AIDAVPENN EEV
Sbjct: 3   SSRGFLQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLEEV 62

Query: 62  ----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYL 121
                     EKV VPANKP+ITISGRR+ DTII+WNDS+NTYNSATLAVLASDFVGRYL
Sbjct: 63  FIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYL 122

Query: 122 TIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN 181
           TIQNG+G GAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN
Sbjct: 123 TIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN 182

Query: 182 SASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV 241
            ASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV
Sbjct: 183 GASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVV 242

Query: 242 FGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPF 301
           FGF++MSDVILPEGWDNWQDPSKQSTVYYGQY+CYGKGANPSRRVPWSFTNMT Q+AAPF
Sbjct: 243 FGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAAPF 302

Query: 302 FTKSFIGAADWLRPLPNHFKRAFSSQP 318
           F+K+FIGAA WLRPLP +FKRAF+S+P
Sbjct: 303 FSKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of PI0004255 vs. NCBI nr
Match: XP_022951672.1 (putative pectinesterase 11 [Cucurbita moschata])

HSP 1 Score: 559.7 bits (1441), Expect = 1.6e-155
Identity = 274/326 (84.05%), Postives = 295/326 (90.49%), Query Frame = 0


Query: 2   SRRSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV- 61
           SR  F    +++  VLVAE TAA A TA+LITVDQSG+GNFTKIQ AIDAVPENN EEV 
Sbjct: 4   SRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLEEVF 63

Query: 62  ---------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYLT 121
                    EKV VPANKP+ITISGRR+ DTII+WNDS+NTYNSATLAVLASDFVGRYLT
Sbjct: 64  IAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLT 123

Query: 122 IQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNS 181
           IQNG+G GAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYY+SCYIEGATDFICGN 
Sbjct: 124 IQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFICGNG 183

Query: 182 ASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 241
           ASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF
Sbjct: 184 ASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 243

Query: 242 GFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFF 301
           GF++MSDVILPEGWDNWQDPSKQSTVYYGQY+CYGKGANPSRRVPWSFTNMT Q+AAPFF
Sbjct: 244 GFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAAPFF 303

Query: 302 TKSFIGAADWLRPLPNHFKRAFSSQP 318
           +K+FIGAA WLRPLP +FKRAF+S+P
Sbjct: 304 SKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of PI0004255 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 344.7 bits (883), Expect = 7.7e-95
Identity = 165/324 (50.93%), Postives = 233/324 (71.91%), Query Frame = 0


Query: 7   LVAISVLAAVLVAEVTAAAA------TTAVLITVDQSGKGNFTKIQEAIDAVPEN-NREE 66
           ++ I +L+++  + + ++++      +TA+LI VDQSGKG+F+KIQEAI+++P N N  +
Sbjct: 20  IINIFILSSITSSSMASSSSPSSIDFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQ 79

Query: 67  V-----------EKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGR 126
           +           EKVV+PA KP+IT+SG +A +T + W+D ++   S TL + ASDFV R
Sbjct: 80  LYFIWVKPGIYREKVVIPAEKPYITLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCR 139

Query: 127 YLTIQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 186
           +LTIQN +G   +AVALRV+ D+ +F  C    +QDTLLDD G HY+K+CYIEGATDFIC
Sbjct: 140 FLTIQNKFGTAGRAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFIC 199

Query: 187 GNSASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 246
           G+++SL+E CHL S+S   G+ITAQ R S +E +GF F+GCK+TG  S  LGRPWGA+SR
Sbjct: 200 GSASSLYERCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSR 259

Query: 247 VVFGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAA 306
           VVF +SF S+V+ P+GW+ W D +K++TVYYG+YKCYG GA+  +RV WS   ++ ++A 
Sbjct: 260 VVFAYSFFSNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWS-KQLSDEEAT 319

Query: 307 PFFTKSFIGAADWLRPLPNHFKRA 313
            F +K FIG  DWLRP P+HFK A
Sbjct: 320 VFLSKDFIGGKDWLRPAPSHFKNA 342

[Close]

BLAST of PI0004255 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 8.8e-75
Identity = 142/294 (48.30%), Postives = 187/294 (63.61%), Query Frame = 0


Query: 36  QSGKGNFTKIQEAIDAVPENNREEV----------EKVVVPANKPFITISGRRAVDTIIS 95
           +S KG+FTKIQ+AID++P  N   V          EKV +P  K FITI G  A  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 96  WNDS----------KNTYNSATLAVLASDFVGRYLTIQNGYG---PGA---QAVALRVSG 155
           W D+            TYNSA+ AV +  FV + +T +N      PGA   QAVALRVS 
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 156 DRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNSASLFENCHLRSVSDAVGT 215
           D  +F  CR LG QDTL D +GRHYYK CYIEG+ DFI GN+ SL+E CH+ +++D +G 
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 216 ITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFSFMSDVILPEGWDNWQ 275
           +TAQ R S  E+TGF F+ CK+TG     LGR WG FSRVVF +++M ++ILP GW NW 
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 276 DPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFFTKSFIGAADWLR 304
           DPS++ TV+YGQYKC G GAN   RV W+   +T ++A PF + +FI  ++W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWA-RELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of PI0004255 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-64
Identity = 130/301 (43.19%), Postives = 183/301 (60.80%), Query Frame = 0


Query: 28  TAVLITVDQSGKGNFTKIQEAIDAVPE----------NNREEVEKVVVPANKPFITISGR 87
           T++++TVD  G GNF+ +Q AID VP+          N+    EKV V  NK  + I GR
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 88  RAVDTIISWNDSK----NTYNSATLAVLASDFVGRYLTIQNGY---GPG---AQAVALRV 147
              +T I WND+     NT +S +  V A++F    ++ +N      PG   AQAVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 148 SGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNSASLFENCHLRSV---- 207
            GD+ +F  C F G QDTLLDD GRH++K C+I+G+ DFI GN  SL+++C + S+    
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 208 -SDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFSFMSDVILP 267
            S   G+ITAQ R+S  E +GF F+ CKI G    +LGR WGA++ VVF  ++MS +I P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 268 EGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFFTKSFIGAADWL 304
           EGW+NW D +K+ TV +G++KCYG GA+   RV +    +T  +A+ F   SFI   +WL
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFG-KQLTDSEASSFIDVSFIDGDEWL 386

[Close]

BLAST of PI0004255 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.3e-62
Identity = 118/301 (39.20%), Postives = 170/301 (56.48%), Query Frame = 0


Query: 31  LITVDQSGKGNFTKIQEAIDAVPENNREEV----------EKVVVPANKPFITISGRRAV 90
           +ITV  +G   F  +Q+A+D++P+NN + +          EKVVVPA KP+IT  G    
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 91  DTIISWNDSKN----------TYNSATLAVLASDFVGRYLTIQNGYG------PGAQAVA 150
            T I W+D  +          TY +A++ V A+ F  R ++  N          G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 151 LRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNSASLFENCHLRSVS 210
            R+SGD+  F+ C F G QDTL DD GRHY+K CYIEG+ DFI GN  S++++C L S++
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 211 DAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFSFMSDVILPEG 270
              G+I A  R  P E TGF F+GC++TG     +GR  G +SR+V+ +++   ++   G
Sbjct: 240 SRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGG 299

Query: 271 WDNWQDPS-KQSTVYYGQYKCYGKGANPSRRVPWSFTNMTAQDAAPFFTKSFIGAADWLR 305
           WD+W   S K  T ++G Y CYG GA  +R V W+   +  + A PF  KSF+    W+ 
Sbjct: 300 WDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWA-RALDYESAHPFIAKSFVNGRHWIA 359

[Close]

BLAST of PI0004255 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.6e-60
Identity = 126/327 (38.53%), Postives = 181/327 (55.35%), Query Frame = 0


Query: 4   RSFLVAISVLAAVLVAEVTAAAATTAVLITVDQSGKGNFTKIQEAIDAVPENNREEV--- 63
           RSF +  +       A   A A     +I VD++G G+   +Q A+D VP++N + V   
Sbjct: 54  RSFSIKENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIF 113

Query: 64  -------EKVVVPANKPFITISGRR--AVDTIISWNDSKN----------TYNSATLAVL 123
                  EKV+VP +KP+I+  G    A DT+ISW+D  +          TY +A++++ 
Sbjct: 114 ILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIE 173

Query: 124 ASDFVGRYLTIQN-----GYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYK 183
           +  F    +T +N         G QAVALR+ GD+  F   R LG QDTL DD G HY+ 
Sbjct: 174 SDFFCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFY 233

Query: 184 SCYIEGATDFICGNSASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINS 243
            CYI+G  DFI GN+ SL+++C + S +   G I A  R+S +E+TGF F+ C I+G   
Sbjct: 234 QCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQ 293

Query: 244 AVLGRPWGAFSRVVFGFSFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVP 303
             LGR WG +SR V+   F++D+I P GW +W+ P +Q  V +G+Y C G+GA    RVP
Sbjct: 294 IYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVP 353

The following BLAST results are available for this feature:

BLAST of PI0004255 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIJ9	1.1e-93	50.93	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
Q8VYZ3	1.2e-73	48.30	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9ZQA3	2.6e-63	43.19	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q8LPF3	1.9e-61	39.20	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9FM79	2.3e-59	38.53	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]

BLAST of PI0004255 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPB4	9.0e-168	91.74	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G361760 PE=4 SV=1	[more]
A0A1S3BBW3	1.7e-166	91.72	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103487980 PE=4 SV=1	[more]
A0A6J1GIB5	7.9e-156	84.05	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454415 PE=4 SV=1	[more]
A0A6J1KQF0	2.3e-155	83.74	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496269 PE=4 SV=1	[more]
A0A6J1CQY5	4.3e-154	82.28	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013943 PE=4 SV=1	[more]

BLAST of PI0004255 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152775.1	1.9e-167	91.74	putative pectinesterase 11 [Cucumis sativus] >KGN62612.1 hypothetical protein Cs...	[more]
XP_008444730.1	3.5e-166	91.72	PREDICTED: putative pectinesterase 11 [Cucumis melo]	[more]
XP_038884203.1	1.5e-164	89.36	putative pectinesterase 11 [Benincasa hispida]	[more]
XP_023536774.1	9.6e-156	84.71	putative pectinesterase 11 [Cucurbita pepo subsp. pepo]	[more]
XP_022951672.1	1.6e-155	84.05	putative pectinesterase 11 [Cucurbita moschata]	[more]

BLAST of PI0004255 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21610.1	7.7e-95	50.93	pectinesterase 11	[more]
AT5G19730.1	8.8e-75	48.30	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	1.8e-64	43.19	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	1.3e-62	39.20	Pectin lyase-like superfamily protein	[more]
AT5G55590.1	1.6e-60	38.53	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 32..297 e-value: 2.7E-64 score: 216.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 26..311 e-value: 1.6E-98 score: 332.0
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 8..303
None	No IPR available	PANTHER	PTHR31321:SF77	PECTINESTERASE 11-RELATED	coord: 8..303
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 26..309

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0004255.1	PI0004255.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity