PI0004080 (gene) Melon (PI 482460) v1

BLAST of PI0004080 vs. ExPASy Swiss-Prot
Match: Q9SVU4 (Tetraspanin-20 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH1 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 3.5e-72
Identity = 145/280 (51.79%), Postives = 191/280 (68.21%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M   CCHVS A ILK  NFLQAF+G+SII+YS WMLD++NHHVPV PPP  P  A SP +
Sbjct: 1   MRHNCCHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDS 60

Query: 61  SSVSFYLNSESVTVADRI-TAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGV 120
           S        E  +V+D +   ++  +  +      +    L +  LPAPWFIY FM  G+
Sbjct: 61  SYSFSNSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGI 120

Query: 121 LLCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGEL 180
           L+C +T++G IAAEAI+GCCLCFY++L  L II+E ALV FI IDR WEKDLP DPTGEL
Sbjct: 121 LVCIVTIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGEL 180

Query: 181 DELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEV-GVRDRTR 240
           + LR FIE+N+D+ KW+GIV+++ Q LSLLLA++LR+MVS R++E D E++      R R
Sbjct: 181 NSLRAFIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQSELDDEDDFENPMSRAR 240

Query: 241 EPLLNPPANQ--AVGAHFDLWGARMREKYGLNNSDRYNSS 277
           + LL P ANQ  +  ++ D W +R+REKYGL NS  +  S
Sbjct: 241 DNLLGPQANQTSSGSSNIDNWRSRIREKYGLINSQSHTPS 280

BLAST of PI0004080 vs. ExPASy Swiss-Prot
Match: Q93XY5 (Tetraspanin-18 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH2 PE=1 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 9.6e-70
Identity = 145/277 (52.35%), Postives = 184/277 (66.43%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M R CCHVS A  LK  NF+QAF+GVSII+YS WML  ++ H+PV PPP   + + +  A
Sbjct: 1   MRRNCCHVSFASTLKILNFVQAFIGVSIIIYSIWMLHEYSRHLPVDPPPSASSSSGTEIA 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
           +SVS  L +    VA  I   N            +    L +  LPAPWFIYSFM  G+L
Sbjct: 61  TSVSEPLKNPIDFVASIILGSN----------GGDHGFNLRSLDLPAPWFIYSFMAVGIL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           +C +T +G IAAEAI+GCCLCFY++L  L I++E ALVA+IAIDR WEKDLP DPTGEL 
Sbjct: 121 VCIVTFIGFIAAEAINGCCLCFYSILKTLLILLEAALVAYIAIDRHWEKDLPYDPTGELS 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEE-EVGVRDRTRE 240
            LR FIE+N+D+ KW+GI +++ Q LSLLLA++LR+MVST K E D EE +   R RT +
Sbjct: 181 SLRAFIEENIDICKWVGIAVVAVQLLSLLLAMVLRAMVSTPKPELDEEEDDENPRSRTWD 240

Query: 241 PLLNPPANQAV--GAHFDLWGARMREKYGLNNSDRYN 275
           PLL P  NQA    +  + W +R+REKYGLN S   N
Sbjct: 241 PLLGPQGNQAPAGSSKIENWSSRIREKYGLNQSPPVN 267

BLAST of PI0004080 vs. ExPASy Swiss-Prot
Match: Q9C5W7 (Tobamovirus multiplication protein 2A OS=Arabidopsis thaliana OX=3702 GN=TOM2A PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.8e-26
Identity = 88/285 (30.88%), Postives = 136/285 (47.72%), Query Frame = 0

Query: 6   CHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAASSVSF 65
           C   L  +LK  NFL A  G+ +I Y  ++   +                     +SV+F
Sbjct: 3   CRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKR----------------VTDNSVTF 62

Query: 66  YLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAF-KLPAPWFIYSFMGFGVLLCCI 125
            L +   +       + +A           + L  N F  LP  WFIY F+G GV L  I
Sbjct: 63  DLTNGDQSYVSFGRPILMA-----------VSLSSNIFDNLPKAWFIYLFIGIGVALFVI 122

Query: 126 TLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELDELRQ 185
           +  GC+   + S CCL  Y++L++L I+VE+   AFI  D  W  +LP D TG  D +  
Sbjct: 123 SCCGCVGTCSRSVCCLSCYSLLLILLILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYN 182

Query: 186 FIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEE-VGVRDRTREPLLN 245
           F+ +N  + +W+ +  +  +AL  LLA+++R+  +    EYD+++E +  R + R+P +N
Sbjct: 183 FLRENWKIVRWVALGAVVFEALLFLLALMVRA--ANTPAEYDSDDEYLAPRQQIRQPFIN 242

Query: 246 PPANQAVG-----------AHFDLWGARMREKYGLNNSD-RYNSS 277
             A    G           +  D W ARMREKYGL+ S+  YN S
Sbjct: 243 RQAAPVTGVPVAPTLDQRPSRSDPWSARMREKYGLDTSEFTYNPS 258

BLAST of PI0004080 vs. ExPASy Swiss-Prot
Match: Q940P5 (Tetraspanin-19 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH3 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.5e-25
Identity = 56/128 (43.75%), Postives = 84/128 (65.62%), Query Frame = 0

Query: 106 PAPWFIYSFMGFGVLLCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDR 165
           P PWFIYSF+G G +LC +T  G IAAE ++GCCL  Y   I+L  +VE  +VA I ++R
Sbjct: 53  PVPWFIYSFLGLGAILCVVTCAGHIAAETVNGCCLYLYMGFIVLLTMVEGGVVADIFLNR 112

Query: 166 RWEKDLPLDPTGELDELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEY 225
            W+KD P DP+G   +  +FIE N  + KWIG+ ++  Q LS+L+A++L+++       Y
Sbjct: 113 DWKKDFPEDPSGAFHQFSKFIESNFKICKWIGLSIVCVQGLSVLIAMLLKALGPHPHRHY 172

Query: 226 DTEEEVGV 234
           D+++E  V
Sbjct: 173 DSDDEYNV 180

BLAST of PI0004080 vs. ExPASy TrEMBL
Match: A0A0A0L8P5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G622440 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.1e-148
Identity = 269/280 (96.07%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M RTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA
Sbjct: 1   MARTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
           SSVSFYLNSESVTVADRITAM+LAADFIP+FD EELKLELNAFKLPAPWFIYSFMGFGVL
Sbjct: 61  SSVSFYLNSESVTVADRITAMDLAADFIPEFDREELKLELNAFKLPAPWFIYSFMGFGVL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEV LVAFIAIDR WEKDLPLDPTGELD
Sbjct: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVGLVAFIAIDRSWEKDLPLDPTGELD 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240
           ELR FIEDN++LSKWIGIV+ISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP
Sbjct: 181 ELRHFIEDNINLSKWIGIVVISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240

Query: 241 LLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQSS 281
           LLNPPANQA GAHFDLWGARMREKYGLNNSDRYNSSNQSS
Sbjct: 241 LLNPPANQAAGAHFDLWGARMREKYGLNNSDRYNSSNQSS 280

BLAST of PI0004080 vs. ExPASy TrEMBL
Match: A0A1S3BH71 (tetraspanin-18 OS=Cucumis melo OX=3656 GN=LOC103489821 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.1e-148
Identity = 268/280 (95.71%), Postives = 276/280 (98.57%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M RTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA
Sbjct: 1   MDRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
           SSVSFYLNSESVTVADRITAM++AADFIP+F+ EELKLELNAFKLPAPWFIYSFMGFGVL
Sbjct: 61  SSVSFYLNSESVTVADRITAMDIAADFIPEFNMEELKLELNAFKLPAPWFIYSFMGFGVL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD
Sbjct: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240
           ELRQFIEDN++LSKWIGIV+ISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP
Sbjct: 181 ELRQFIEDNINLSKWIGIVVISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240

Query: 241 LLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQSS 281
           LLNPPANQA GAHFDLWGARMREKYG NNSDRYNSSNQS+
Sbjct: 241 LLNPPANQAAGAHFDLWGARMREKYGFNNSDRYNSSNQST 280

BLAST of PI0004080 vs. ExPASy TrEMBL
Match: A0A5A7T8U7 (Tetraspanin-18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold487G00590 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.0e-147
Identity = 267/279 (95.70%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M RTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA
Sbjct: 1   MDRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
           SSVSFYLNSESVTVADRITAM++AADFIP+F+ EELKLELNAFKLPAPWFIYSFMGFGVL
Sbjct: 61  SSVSFYLNSESVTVADRITAMDIAADFIPEFNMEELKLELNAFKLPAPWFIYSFMGFGVL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD
Sbjct: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240
           ELRQFIEDN++LSKWIGIV+ISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP
Sbjct: 181 ELRQFIEDNINLSKWIGIVVISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240

Query: 241 LLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQS 280
           LLNPPANQA GAHFDLWGARMREKYG NNSDRYNSSNQ+
Sbjct: 241 LLNPPANQAAGAHFDLWGARMREKYGFNNSDRYNSSNQN 279

BLAST of PI0004080 vs. ExPASy TrEMBL
Match: A0A6J1HHZ4 (tetraspanin-20-like OS=Cucurbita moschata OX=3662 GN=LOC111463811 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 4.9e-133
Identity = 246/281 (87.54%), Postives = 261/281 (92.88%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPP-PVPALAPSPA 60
           M RTCCHVSLAFI+KCFNFLQAFVGVSIIVYSAWMLDRWNHH+PVSPPP P+PALAPSPA
Sbjct: 1   MARTCCHVSLAFIIKCFNFLQAFVGVSIIVYSAWMLDRWNHHLPVSPPPSPLPALAPSPA 60

Query: 61  ASSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGV 120
               SFYL+SESV VADRI  +NLAAD IP FDT EL+++LNAFKLPAPWFIYSFMG G+
Sbjct: 61  ----SFYLSSESVAVADRIMPINLAADLIPGFDT-ELEIDLNAFKLPAPWFIYSFMGLGI 120

Query: 121 LLCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGEL 180
           LLCCI LVGCIAAEAISGCCLCFYNMLIMLFI+VEVALVAFI IDRRWEKDLPLDPTGEL
Sbjct: 121 LLCCIALVGCIAAEAISGCCLCFYNMLIMLFILVEVALVAFIGIDRRWEKDLPLDPTGEL 180

Query: 181 DELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTRE 240
            ELRQFIEDN+D+ KWIGI++ISTQALSLLLAIILRSMVSTRKTEYDTEEE+GVRDRTRE
Sbjct: 181 KELRQFIEDNIDVCKWIGIIVISTQALSLLLAIILRSMVSTRKTEYDTEEEIGVRDRTRE 240

Query: 241 PLLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQSS 281
           PLLNPP NQA GAHFDLWGARMREKYGLNNSDRYNSSNQ S
Sbjct: 241 PLLNPPGNQASGAHFDLWGARMREKYGLNNSDRYNSSNQIS 276

BLAST of PI0004080 vs. ExPASy TrEMBL
Match: A0A6J1HWE7 (tetraspanin-20-like OS=Cucurbita maxima OX=3661 GN=LOC111466873 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.4e-132
Identity = 245/281 (87.19%), Postives = 259/281 (92.17%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPP-PVPALAPSPA 60
           M RTCCHVSLAFI+KCFNFLQAFVGVSIIVYSAWMLDRWNHH+PVSPPP P+PALAPSPA
Sbjct: 1   MARTCCHVSLAFIIKCFNFLQAFVGVSIIVYSAWMLDRWNHHLPVSPPPSPLPALAPSPA 60

Query: 61  ASSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGV 120
               SFYL+SESV VADRI  +NLAAD  P FDT EL+++LNAFKLPAPWFIYSFMG G+
Sbjct: 61  ----SFYLSSESVAVADRIMPINLAADLTPGFDT-ELEIDLNAFKLPAPWFIYSFMGLGI 120

Query: 121 LLCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGEL 180
           LLCCI LVGCIAAEAISGCCLCFYNMLIMLFI+VEVALVAFI IDRRWEKDLPLDPTGEL
Sbjct: 121 LLCCIALVGCIAAEAISGCCLCFYNMLIMLFILVEVALVAFIGIDRRWEKDLPLDPTGEL 180

Query: 181 DELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTRE 240
           +ELRQFIEDN+DL KWIGI +ISTQALSLLLAIILRSMVSTRKTEYDTEEE+G RDRTRE
Sbjct: 181 EELRQFIEDNIDLCKWIGITVISTQALSLLLAIILRSMVSTRKTEYDTEEEIGARDRTRE 240

Query: 241 PLLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQSS 281
           PLLNPP NQA GAHFDLWGARMREKYGLNNSDRYNSSNQ S
Sbjct: 241 PLLNPPGNQASGAHFDLWGARMREKYGLNNSDRYNSSNQIS 276

BLAST of PI0004080 vs. NCBI nr
Match: XP_004152011.1 (tetraspanin-18 [Cucumis sativus] >KGN58325.1 hypothetical protein Csa_017444 [Cucumis sativus])

HSP 1 Score: 532.7 bits (1371), Expect = 1.9e-147
Identity = 269/280 (96.07%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M RTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA
Sbjct: 1   MARTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
           SSVSFYLNSESVTVADRITAM+LAADFIP+FD EELKLELNAFKLPAPWFIYSFMGFGVL
Sbjct: 61  SSVSFYLNSESVTVADRITAMDLAADFIPEFDREELKLELNAFKLPAPWFIYSFMGFGVL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEV LVAFIAIDR WEKDLPLDPTGELD
Sbjct: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVGLVAFIAIDRSWEKDLPLDPTGELD 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240
           ELR FIEDN++LSKWIGIV+ISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP
Sbjct: 181 ELRHFIEDNINLSKWIGIVVISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240

Query: 241 LLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQSS 281
           LLNPPANQA GAHFDLWGARMREKYGLNNSDRYNSSNQSS
Sbjct: 241 LLNPPANQAAGAHFDLWGARMREKYGLNNSDRYNSSNQSS 280

BLAST of PI0004080 vs. NCBI nr
Match: XP_008447349.1 (PREDICTED: tetraspanin-18 [Cucumis melo])

HSP 1 Score: 532.7 bits (1371), Expect = 1.9e-147
Identity = 268/280 (95.71%), Postives = 276/280 (98.57%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M RTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA
Sbjct: 1   MDRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
           SSVSFYLNSESVTVADRITAM++AADFIP+F+ EELKLELNAFKLPAPWFIYSFMGFGVL
Sbjct: 61  SSVSFYLNSESVTVADRITAMDIAADFIPEFNMEELKLELNAFKLPAPWFIYSFMGFGVL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD
Sbjct: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240
           ELRQFIEDN++LSKWIGIV+ISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP
Sbjct: 181 ELRQFIEDNINLSKWIGIVVISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240

Query: 241 LLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQSS 281
           LLNPPANQA GAHFDLWGARMREKYG NNSDRYNSSNQS+
Sbjct: 241 LLNPPANQAAGAHFDLWGARMREKYGFNNSDRYNSSNQST 280

BLAST of PI0004080 vs. NCBI nr
Match: KAA0037895.1 (tetraspanin-18 [Cucumis melo var. makuwa] >TYJ98029.1 tetraspanin-18 [Cucumis melo var. makuwa])

HSP 1 Score: 531.6 bits (1368), Expect = 4.2e-147
Identity = 267/279 (95.70%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M RTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA
Sbjct: 1   MDRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
           SSVSFYLNSESVTVADRITAM++AADFIP+F+ EELKLELNAFKLPAPWFIYSFMGFGVL
Sbjct: 61  SSVSFYLNSESVTVADRITAMDIAADFIPEFNMEELKLELNAFKLPAPWFIYSFMGFGVL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD
Sbjct: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240
           ELRQFIEDN++LSKWIGIV+ISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP
Sbjct: 181 ELRQFIEDNINLSKWIGIVVISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240

Query: 241 LLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQS 280
           LLNPPANQA GAHFDLWGARMREKYG NNSDRYNSSNQ+
Sbjct: 241 LLNPPANQAAGAHFDLWGARMREKYGFNNSDRYNSSNQN 279

BLAST of PI0004080 vs. NCBI nr
Match: XP_038888178.1 (tetraspanin-20 [Benincasa hispida])

HSP 1 Score: 498.8 bits (1283), Expect = 3.0e-137
Identity = 254/280 (90.71%), Postives = 266/280 (95.00%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           MGRTCCHVSLAFILKCF+FLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPP+P LAPSP A
Sbjct: 1   MGRTCCHVSLAFILKCFSFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPIPDLAPSP-A 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
            SVSFYLNSESV V DRITAMNLA +  P+F+T EL+L+LNAFKLPAPWFIYSFMGFG+L
Sbjct: 61  RSVSFYLNSESVAVNDRITAMNLATNLAPEFET-ELELDLNAFKLPAPWFIYSFMGFGIL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           LCCITLVGCIAAEAISGCCLCFYNMLIMLFI+VEVALVAFIAIDRRWEKDLPLDPTGELD
Sbjct: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFILVEVALVAFIAIDRRWEKDLPLDPTGELD 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTREP 240
            L QFIEDN+DL KWIGIV+ISTQALSLLLAIILRSMVSTRKTEYDTEEEVG +DRTREP
Sbjct: 181 GLWQFIEDNIDLCKWIGIVVISTQALSLLLAIILRSMVSTRKTEYDTEEEVGAQDRTREP 240

Query: 241 LLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQSS 281
           LLNPPANQA GAHFDLWGARMREKYGLNNSDRYNSSNQSS
Sbjct: 241 LLNPPANQASGAHFDLWGARMREKYGLNNSDRYNSSNQSS 278

BLAST of PI0004080 vs. NCBI nr
Match: KAG6571566.1 (Tetraspanin-18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 483.8 bits (1244), Expect = 1.0e-132
Identity = 246/281 (87.54%), Postives = 261/281 (92.88%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPP-PVPALAPSPA 60
           M RTCCHVSLAFI+KCFNFLQAFVGVSIIVYSAWMLDRWNHH+PVSPPP P+PALAPSPA
Sbjct: 1   MARTCCHVSLAFIIKCFNFLQAFVGVSIIVYSAWMLDRWNHHLPVSPPPSPLPALAPSPA 60

Query: 61  ASSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGV 120
               SFYL+SESV VADRI  +NLAAD IP FDT EL+++LNAFKLPAPWFIYSFMG G+
Sbjct: 61  ----SFYLSSESVAVADRIMPINLAADSIPGFDT-ELEIDLNAFKLPAPWFIYSFMGLGI 120

Query: 121 LLCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGEL 180
           LLCCI LVGCIAAEAISGCCLCFYNMLIMLFI+VEVALVAFI IDRRWEKDLPLDPTGEL
Sbjct: 121 LLCCIALVGCIAAEAISGCCLCFYNMLIMLFILVEVALVAFIGIDRRWEKDLPLDPTGEL 180

Query: 181 DELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEVGVRDRTRE 240
           +ELRQFIEDN+DL KWIGI++ISTQALSLLLAIILRSMVSTRKTEYDTEEE+G RDRTRE
Sbjct: 181 EELRQFIEDNIDLCKWIGIIVISTQALSLLLAIILRSMVSTRKTEYDTEEEIGARDRTRE 240

Query: 241 PLLNPPANQAVGAHFDLWGARMREKYGLNNSDRYNSSNQSS 281
           PLLNPP NQA GAHFDLWGARMREKYGLNNSDRYNSSNQ S
Sbjct: 241 PLLNPPGNQASGAHFDLWGARMREKYGLNNSDRYNSSNQIS 276

BLAST of PI0004080 vs. TAIR 10
Match: AT4G28770.1 (Tetraspanin family protein )

HSP 1 Score: 273.1 bits (697), Expect = 2.5e-73
Identity = 145/280 (51.79%), Postives = 191/280 (68.21%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M   CCHVS A ILK  NFLQAF+G+SII+YS WMLD++NHHVPV PPP  P  A SP +
Sbjct: 1   MRHNCCHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDS 60

Query: 61  SSVSFYLNSESVTVADRI-TAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGV 120
           S        E  +V+D +   ++  +  +      +    L +  LPAPWFIY FM  G+
Sbjct: 61  SYSFSNSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGI 120

Query: 121 LLCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGEL 180
           L+C +T++G IAAEAI+GCCLCFY++L  L II+E ALV FI IDR WEKDLP DPTGEL
Sbjct: 121 LVCIVTIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGEL 180

Query: 181 DELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEV-GVRDRTR 240
           + LR FIE+N+D+ KW+GIV+++ Q LSLLLA++LR+MVS R++E D E++      R R
Sbjct: 181 NSLRAFIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQSELDDEDDFENPMSRAR 240

Query: 241 EPLLNPPANQ--AVGAHFDLWGARMREKYGLNNSDRYNSS 277
           + LL P ANQ  +  ++ D W +R+REKYGL NS  +  S
Sbjct: 241 DNLLGPQANQTSSGSSNIDNWRSRIREKYGLINSQSHTPS 280

BLAST of PI0004080 vs. TAIR 10
Match: AT4G28770.2 (Tetraspanin family protein )

HSP 1 Score: 273.1 bits (697), Expect = 2.5e-73
Identity = 145/280 (51.79%), Postives = 191/280 (68.21%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M   CCHVS A ILK  NFLQAF+G+SII+YS WMLD++NHHVPV PPP  P  A SP +
Sbjct: 45  MRHNCCHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDS 104

Query: 61  SSVSFYLNSESVTVADRI-TAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGV 120
           S        E  +V+D +   ++  +  +      +    L +  LPAPWFIY FM  G+
Sbjct: 105 SYSFSNSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGI 164

Query: 121 LLCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGEL 180
           L+C +T++G IAAEAI+GCCLCFY++L  L II+E ALV FI IDR WEKDLP DPTGEL
Sbjct: 165 LVCIVTIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGEL 224

Query: 181 DELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEEV-GVRDRTR 240
           + LR FIE+N+D+ KW+GIV+++ Q LSLLLA++LR+MVS R++E D E++      R R
Sbjct: 225 NSLRAFIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQSELDDEDDFENPMSRAR 284

Query: 241 EPLLNPPANQ--AVGAHFDLWGARMREKYGLNNSDRYNSS 277
           + LL P ANQ  +  ++ D W +R+REKYGL NS  +  S
Sbjct: 285 DNLLGPQANQTSSGSSNIDNWRSRIREKYGLINSQSHTPS 324

BLAST of PI0004080 vs. TAIR 10
Match: AT2G20230.1 (Tetraspanin family protein )

HSP 1 Score: 265.0 bits (676), Expect = 6.8e-71
Identity = 145/277 (52.35%), Postives = 184/277 (66.43%), Query Frame = 0

Query: 1   MGRTCCHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAA 60
           M R CCHVS A  LK  NF+QAF+GVSII+YS WML  ++ H+PV PPP   + + +  A
Sbjct: 1   MRRNCCHVSFASTLKILNFVQAFIGVSIIIYSIWMLHEYSRHLPVDPPPSASSSSGTEIA 60

Query: 61  SSVSFYLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAFKLPAPWFIYSFMGFGVL 120
           +SVS  L +    VA  I   N            +    L +  LPAPWFIYSFM  G+L
Sbjct: 61  TSVSEPLKNPIDFVASIILGSN----------GGDHGFNLRSLDLPAPWFIYSFMAVGIL 120

Query: 121 LCCITLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELD 180
           +C +T +G IAAEAI+GCCLCFY++L  L I++E ALVA+IAIDR WEKDLP DPTGEL 
Sbjct: 121 VCIVTFIGFIAAEAINGCCLCFYSILKTLLILLEAALVAYIAIDRHWEKDLPYDPTGELS 180

Query: 181 ELRQFIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEE-EVGVRDRTRE 240
            LR FIE+N+D+ KW+GI +++ Q LSLLLA++LR+MVST K E D EE +   R RT +
Sbjct: 181 SLRAFIEENIDICKWVGIAVVAVQLLSLLLAMVLRAMVSTPKPELDEEEDDENPRSRTWD 240

Query: 241 PLLNPPANQAV--GAHFDLWGARMREKYGLNNSDRYN 275
           PLL P  NQA    +  + W +R+REKYGLN S   N
Sbjct: 241 PLLGPQGNQAPAGSSKIENWSSRIREKYGLNQSPPVN 267

BLAST of PI0004080 vs. TAIR 10
Match: AT1G32400.1 (tobamovirus multiplication 2A )

HSP 1 Score: 120.2 bits (300), Expect = 2.7e-27
Identity = 88/285 (30.88%), Postives = 136/285 (47.72%), Query Frame = 0

Query: 6   CHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAASSVSF 65
           C   L  +LK  NFL A  G+ +I Y  ++   +                     +SV+F
Sbjct: 3   CRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKR----------------VTDNSVTF 62

Query: 66  YLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAF-KLPAPWFIYSFMGFGVLLCCI 125
            L +   +       + +A           + L  N F  LP  WFIY F+G GV L  I
Sbjct: 63  DLTNGDQSYVSFGRPILMA-----------VSLSSNIFDNLPKAWFIYLFIGIGVALFVI 122

Query: 126 TLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELDELRQ 185
           +  GC+   + S CCL  Y++L++L I+VE+   AFI  D  W  +LP D TG  D +  
Sbjct: 123 SCCGCVGTCSRSVCCLSCYSLLLILLILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYN 182

Query: 186 FIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEE-VGVRDRTREPLLN 245
           F+ +N  + +W+ +  +  +AL  LLA+++R+  +    EYD+++E +  R + R+P +N
Sbjct: 183 FLRENWKIVRWVALGAVVFEALLFLLALMVRA--ANTPAEYDSDDEYLAPRQQIRQPFIN 242

Query: 246 PPANQAVG-----------AHFDLWGARMREKYGLNNSD-RYNSS 277
             A    G           +  D W ARMREKYGL+ S+  YN S
Sbjct: 243 RQAAPVTGVPVAPTLDQRPSRSDPWSARMREKYGLDTSEFTYNPS 258

BLAST of PI0004080 vs. TAIR 10
Match: AT1G32400.2 (tobamovirus multiplication 2A )

HSP 1 Score: 120.2 bits (300), Expect = 2.7e-27
Identity = 88/285 (30.88%), Postives = 136/285 (47.72%), Query Frame = 0

Query: 6   CHVSLAFILKCFNFLQAFVGVSIIVYSAWMLDRWNHHVPVSPPPPVPALAPSPAASSVSF 65
           C   L  +LK  NFL A  G+ +I Y  ++   +                     +SV+F
Sbjct: 3   CRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKR----------------VTDNSVTF 62

Query: 66  YLNSESVTVADRITAMNLAADFIPQFDTEELKLELNAF-KLPAPWFIYSFMGFGVLLCCI 125
            L +   +       + +A           + L  N F  LP  WFIY F+G GV L  I
Sbjct: 63  DLTNGDQSYVSFGRPILMA-----------VSLSSNIFDNLPKAWFIYLFIGIGVALFVI 122

Query: 126 TLVGCIAAEAISGCCLCFYNMLIMLFIIVEVALVAFIAIDRRWEKDLPLDPTGELDELRQ 185
           +  GC+   + S CCL  Y++L++L I+VE+   AFI  D  W  +LP D TG  D +  
Sbjct: 123 SCCGCVGTCSRSVCCLSCYSLLLILLILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYN 182

Query: 186 FIEDNMDLSKWIGIVMISTQALSLLLAIILRSMVSTRKTEYDTEEE-VGVRDRTREPLLN 245
           F+ +N  + +W+ +  +  +AL  LLA+++R+  +    EYD+++E +  R + R+P +N
Sbjct: 183 FLRENWKIVRWVALGAVVFEALLFLLALMVRA--ANTPAEYDSDDEYLAPRQQIRQPFIN 242

Query: 246 PPANQAVG-----------AHFDLWGARMREKYGLNNSD-RYNSS 277
             A    G           +  D W ARMREKYGL+ S+  YN S
Sbjct: 243 RQAAPVTGVPVAPTLDQRPSRSDPWSARMREKYGLDTSEFTYNPS 258