PI0004077 (gene) Melon (PI 482460) v1

BLAST of PI0004077 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 268.1 bits (684), Expect = 1.2e-70
Identity = 131/241 (54.36%), Postives = 164/241 (68.05%), Query Frame = 0

Query: 20  QQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPPQCSPTPYILRLAADLTWVE 79
           Q P PP  IF+L+GQSNMAGRGGV      +   WD ++PP+C+P   ILRL+ADL W E
Sbjct: 16  QSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEE 75

Query: 80  AREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLVPCAIGGTSIKEWQQGSNLY 139
           A EPLH DID  K  G+GPGM FAN +         VIGLVPCA GGT+IKEW++GS+LY
Sbjct: 76  AHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLY 135

Query: 140 NHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGRLKKFFTDIRSDLKIPLLPI 199
             ++ R + S   GG+IKA+LWYQGESD  +  D+E YG  + +   ++R DL +P LPI
Sbjct: 136 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 195

Query: 200 IQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEPDGLHLTTPSQVRLGGVLADA 259
           IQV IASG G Y + VR+ Q G+ L NV+ VDA GL L+ D LHLTT +QV+LG  LA A
Sbjct: 196 IQVAIASG-GGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQA 255

Query: 260 Y 261
           Y
Sbjct: 256 Y 255

BLAST of PI0004077 vs. ExPASy TrEMBL
Match: A0A0A0L4Z7 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G641680 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 5.4e-154
Identity = 276/298 (92.62%), Postives = 281/298 (94.30%), Query Frame = 0

Query: 1   MLYLCLLFLTTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPP 60
           MLYLCLLFLTT AQIP+TSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHH TWDGVVPP
Sbjct: 1   MLYLCLLFLTTVAQIPSTSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHPTWDGVVPP 60

Query: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120
           QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV
Sbjct: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180
           PCA+GGTSIKEWQ+GSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR
Sbjct: 121 PCAMGGTSIKEWQKGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEPD 240
           LKKFFT IRSDLKIPLLPIIQVGIASGEGEYKEGVR+GQFGIDLVNVMIVDALGL LEPD
Sbjct: 181 LKKFFTGIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEPD 240

Query: 241 GLHLTTPSQVRLGGVLADAYRRFPSHPLATPLTNAAPISIFSTFFLSISWIFTFFLLF 299
           GLHLTT SQVRLGG+LADAYRRFPSHPLATPLTNAAPIS  ST F      F  FL F
Sbjct: 241 GLHLTTTSQVRLGGLLADAYRRFPSHPLATPLTNAAPISRISTIFFP----FVGFLRF 294

BLAST of PI0004077 vs. ExPASy TrEMBL
Match: A0A5A7VA07 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003010 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.1e-153
Identity = 276/301 (91.69%), Postives = 283/301 (94.02%), Query Frame = 0

Query: 1   MLYLCLLFL-TTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVP 60
           MLYLCLLFL TTAAQIP+ SQQPSPPTDIFLLAGQSNMAGRGGVTN TLTH  TWDGVVP
Sbjct: 1   MLYLCLLFLTTTAAQIPSASQQPSPPTDIFLLAGQSNMAGRGGVTNGTLTHQPTWDGVVP 60

Query: 61  PQCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGL 120
           PQCSPTPYILR AADLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKPGGRTVIGL
Sbjct: 61  PQCSPTPYILRFAADLTWVEAREPLHADIDFLKTNGIGPGMPFANAILMDKPGGRTVIGL 120

Query: 121 VPCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGG 180
           VPCAIGGTSIKEWQ+GSNLYNHLLSRA+AS+L GGKIKALLWYQGESDTENAEDSELYGG
Sbjct: 121 VPCAIGGTSIKEWQKGSNLYNHLLSRAEASILRGGKIKALLWYQGESDTENAEDSELYGG 180

Query: 181 RLKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEP 240
           RLKKF TDIRSDLKIPLLPIIQVGIASGEGEYKEGVR+GQFGIDLVNVMIVDALGL LEP
Sbjct: 181 RLKKFLTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEP 240

Query: 241 DGLHLTTPSQVRLGGVLADAYRRFPSHPLATPLTNAAPIS-IFSTFFLSISWIFTFFLLF 300
           DGLHLTTPSQVRLGG+LA AYRRFPSHPLATPLTNAAPIS I STF LSI WIFTF   F
Sbjct: 241 DGLHLTTPSQVRLGGLLAHAYRRFPSHPLATPLTNAAPISTIISTFLLSIWWIFTFLFPF 300

BLAST of PI0004077 vs. ExPASy TrEMBL
Match: A0A1S3BVE3 (probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103493577 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.1e-153
Identity = 276/301 (91.69%), Postives = 283/301 (94.02%), Query Frame = 0

Query: 1   MLYLCLLFL-TTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVP 60
           MLYLCLLFL TTAAQIP+ SQQPSPPTDIFLLAGQSNMAGRGGVTN TLTH  TWDGVVP
Sbjct: 1   MLYLCLLFLTTTAAQIPSASQQPSPPTDIFLLAGQSNMAGRGGVTNGTLTHQPTWDGVVP 60

Query: 61  PQCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGL 120
           PQCSPTPYILR AADLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKPGGRTVIGL
Sbjct: 61  PQCSPTPYILRFAADLTWVEAREPLHADIDFLKTNGIGPGMPFANAILMDKPGGRTVIGL 120

Query: 121 VPCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGG 180
           VPCAIGGTSIKEWQ+GSNLYNHLLSRA+AS+L GGKIKALLWYQGESDTENAEDSELYGG
Sbjct: 121 VPCAIGGTSIKEWQKGSNLYNHLLSRAEASILRGGKIKALLWYQGESDTENAEDSELYGG 180

Query: 181 RLKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEP 240
           RLKKF TDIRSDLKIPLLPIIQVGIASGEGEYKEGVR+GQFGIDLVNVMIVDALGL LEP
Sbjct: 181 RLKKFLTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEP 240

Query: 241 DGLHLTTPSQVRLGGVLADAYRRFPSHPLATPLTNAAPIS-IFSTFFLSISWIFTFFLLF 300
           DGLHLTTPSQVRLGG+LA AYRRFPSHPLATPLTNAAPIS I STF LSI WIFTF   F
Sbjct: 241 DGLHLTTPSQVRLGGLLAHAYRRFPSHPLATPLTNAAPISTIISTFLLSIWWIFTFLFPF 300

BLAST of PI0004077 vs. ExPASy TrEMBL
Match: A0A6J1FS50 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111446742 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.6e-132
Identity = 245/307 (79.80%), Postives = 265/307 (86.32%), Query Frame = 0

Query: 1   MLYLCLLFLTTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPP 60
           MLY  LLFL  AA +P TSQQP PPT IFLLAGQSNMAGRGGVTNSTLTH  TWDGVVPP
Sbjct: 28  MLYFSLLFLLMAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDGVVPP 87

Query: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120
           QCSP P ILRLA+DLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKP GRTVIGLV
Sbjct: 88  QCSPNPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLV 147

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180
           PCA+GGTSI++WQ+GSNLY HLL+RA+AS+LSGG IKALLWYQGESDT NAEDSELYGGR
Sbjct: 148 PCAMGGTSIQQWQKGSNLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGR 207

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVD--ALGLSLE 240
           L KFFTDIRSDL IP LPIIQVGIASGEG YKEGVR+GQ GI+++NVM VD  ALGLS E
Sbjct: 208 LNKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFE 267

Query: 241 PDGLHLTTPSQVRLGGVLADAYRRFPSHPL-ATPLTNAAPISIFSTFFLSISWIFTFFLL 300
           PDGLHL TPSQV+LGGVLADAYRRFP HPL A+PL +AA  +   +FF+S+    TF L 
Sbjct: 268 PDGLHLNTPSQVKLGGVLADAYRRFPPHPLAASPLRSAASTASVHSFFISMFRTLTFVL- 327

Query: 301 FLHLILL 305
            LH ILL
Sbjct: 328 -LHQILL 332

BLAST of PI0004077 vs. ExPASy TrEMBL
Match: A0A6J1J4F1 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111481152 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.1e-130
Identity = 245/307 (79.80%), Postives = 264/307 (85.99%), Query Frame = 0

Query: 1   MLYLCLLFLTTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPP 60
           MLY  LLFL  AA +P TSQQP PPT IFLLAGQSNMAGRGGV+N+TLTH  TWDGVVPP
Sbjct: 1   MLYFSLLFL-MAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVSNTTLTHRPTWDGVVPP 60

Query: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120
           QCSP P ILRLA+DLTWVEAREPLHADIDFLK NGIGPGMPFANTILMDKP GRTVIGLV
Sbjct: 61  QCSPNPSILRLASDLTWVEAREPLHADIDFLKINGIGPGMPFANTILMDKPAGRTVIGLV 120

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180
           PCA+GGTSI++WQ+GSNLYNHLL+RA+AS+L GG IKALLWYQGESDT NAEDSELYGGR
Sbjct: 121 PCAMGGTSIQQWQKGSNLYNHLLTRAEASILGGGTIKALLWYQGESDTVNAEDSELYGGR 180

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVD--ALGLSLE 240
           LKKFFTDIRSDL IP LPIIQVGIASGEG YKEGVR+GQFGI+++NVM VD  ALGLS E
Sbjct: 181 LKKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQFGIEVMNVMTVDAYALGLSFE 240

Query: 241 PDGLHLTTPSQVRLGGVLADAYRRFPSHPL-ATPLTNAAPISIFSTFFLSISWIFTFFLL 300
           PDGLHL TPSQV+LGGVLADAYRRFP HPL A+PL NAA  S  S +   IS   T   +
Sbjct: 241 PDGLHLNTPSQVKLGGVLADAYRRFPPHPLAASPLRNAA--STASVYSFCISMFRTMTFV 300

Query: 301 FLHLILL 305
           FLH ILL
Sbjct: 301 FLHQILL 304

BLAST of PI0004077 vs. NCBI nr
Match: XP_004141392.1 (probable carbohydrate esterase At4g34215 [Cucumis sativus] >KGN55236.1 hypothetical protein Csa_012338 [Cucumis sativus])

HSP 1 Score: 553.5 bits (1425), Expect = 1.1e-153
Identity = 276/298 (92.62%), Postives = 281/298 (94.30%), Query Frame = 0

Query: 1   MLYLCLLFLTTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPP 60
           MLYLCLLFLTT AQIP+TSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHH TWDGVVPP
Sbjct: 1   MLYLCLLFLTTVAQIPSTSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHPTWDGVVPP 60

Query: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120
           QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV
Sbjct: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180
           PCA+GGTSIKEWQ+GSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR
Sbjct: 121 PCAMGGTSIKEWQKGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEPD 240
           LKKFFT IRSDLKIPLLPIIQVGIASGEGEYKEGVR+GQFGIDLVNVMIVDALGL LEPD
Sbjct: 181 LKKFFTGIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEPD 240

Query: 241 GLHLTTPSQVRLGGVLADAYRRFPSHPLATPLTNAAPISIFSTFFLSISWIFTFFLLF 299
           GLHLTT SQVRLGG+LADAYRRFPSHPLATPLTNAAPIS  ST F      F  FL F
Sbjct: 241 GLHLTTTSQVRLGGLLADAYRRFPSHPLATPLTNAAPISRISTIFFP----FVGFLRF 294

BLAST of PI0004077 vs. NCBI nr
Match: XP_008452605.1 (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo] >KAA0064418.1 putative carbohydrate esterase [Cucumis melo var. makuwa] >TYK20169.1 putative carbohydrate esterase [Cucumis melo var. makuwa])

HSP 1 Score: 551.6 bits (1420), Expect = 4.3e-153
Identity = 276/301 (91.69%), Postives = 283/301 (94.02%), Query Frame = 0

Query: 1   MLYLCLLFL-TTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVP 60
           MLYLCLLFL TTAAQIP+ SQQPSPPTDIFLLAGQSNMAGRGGVTN TLTH  TWDGVVP
Sbjct: 1   MLYLCLLFLTTTAAQIPSASQQPSPPTDIFLLAGQSNMAGRGGVTNGTLTHQPTWDGVVP 60

Query: 61  PQCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGL 120
           PQCSPTPYILR AADLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKPGGRTVIGL
Sbjct: 61  PQCSPTPYILRFAADLTWVEAREPLHADIDFLKTNGIGPGMPFANAILMDKPGGRTVIGL 120

Query: 121 VPCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGG 180
           VPCAIGGTSIKEWQ+GSNLYNHLLSRA+AS+L GGKIKALLWYQGESDTENAEDSELYGG
Sbjct: 121 VPCAIGGTSIKEWQKGSNLYNHLLSRAEASILRGGKIKALLWYQGESDTENAEDSELYGG 180

Query: 181 RLKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEP 240
           RLKKF TDIRSDLKIPLLPIIQVGIASGEGEYKEGVR+GQFGIDLVNVMIVDALGL LEP
Sbjct: 181 RLKKFLTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVDALGLPLEP 240

Query: 241 DGLHLTTPSQVRLGGVLADAYRRFPSHPLATPLTNAAPIS-IFSTFFLSISWIFTFFLLF 300
           DGLHLTTPSQVRLGG+LA AYRRFPSHPLATPLTNAAPIS I STF LSI WIFTF   F
Sbjct: 241 DGLHLTTPSQVRLGGLLAHAYRRFPSHPLATPLTNAAPISTIISTFLLSIWWIFTFLFPF 300

BLAST of PI0004077 vs. NCBI nr
Match: XP_038899610.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 548.1 bits (1411), Expect = 4.7e-152
Identity = 277/304 (91.12%), Postives = 287/304 (94.41%), Query Frame = 0

Query: 1   MLYLCLLFLTTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPP 60
           MLYLCLLFL TAAQIP TSQQPSPPT IFLLAGQSNMAGRGGVTNST+TH  TWDGVVPP
Sbjct: 1   MLYLCLLFL-TAAQIPVTSQQPSPPTAIFLLAGQSNMAGRGGVTNSTVTHRPTWDGVVPP 60

Query: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120
           QCSPTP ILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFA+TILMDKPGGRTVIGLV
Sbjct: 61  QCSPTPSILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFAHTILMDKPGGRTVIGLV 120

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180
           PCAIGGTSIKEWQQGSNLYNHLLSRA+ASVLSGGKIKALLWYQGESDTENAEDSELYG R
Sbjct: 121 PCAIGGTSIKEWQQGSNLYNHLLSRAEASVLSGGKIKALLWYQGESDTENAEDSELYGSR 180

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEPD 240
           LKKFFTDIRSDLKIPLLPIIQVGIA+GEG YKEGVR+GQFGIDLVNVM VDA+GLSLEPD
Sbjct: 181 LKKFFTDIRSDLKIPLLPIIQVGIATGEGPYKEGVRRGQFGIDLVNVMTVDAMGLSLEPD 240

Query: 241 GLHLTTPSQVRLGGVLADAYRRFPSHPLATPLTNAAPISIFSTFFLSISWIFTFFLLFLH 300
           GLHLTTPSQV+LGG+LADAYRRFPSHPLATPLTNAA I + STFFLSIS I T  LLFL 
Sbjct: 241 GLHLTTPSQVQLGGLLADAYRRFPSHPLATPLTNAAHIPMISTFFLSISRILTVVLLFLR 300

Query: 301 LILL 305
           LIL+
Sbjct: 301 LILM 303

BLAST of PI0004077 vs. NCBI nr
Match: XP_022941448.1 (probable carbohydrate esterase At4g34215 [Cucurbita moschata])

HSP 1 Score: 481.5 bits (1238), Expect = 5.4e-132
Identity = 245/307 (79.80%), Postives = 265/307 (86.32%), Query Frame = 0

Query: 1   MLYLCLLFLTTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPP 60
           MLY  LLFL  AA +P TSQQP PPT IFLLAGQSNMAGRGGVTNSTLTH  TWDGVVPP
Sbjct: 28  MLYFSLLFLLMAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDGVVPP 87

Query: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120
           QCSP P ILRLA+DLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKP GRTVIGLV
Sbjct: 88  QCSPNPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLV 147

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180
           PCA+GGTSI++WQ+GSNLY HLL+RA+AS+LSGG IKALLWYQGESDT NAEDSELYGGR
Sbjct: 148 PCAMGGTSIQQWQKGSNLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGR 207

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVD--ALGLSLE 240
           L KFFTDIRSDL IP LPIIQVGIASGEG YKEGVR+GQ GI+++NVM VD  ALGLS E
Sbjct: 208 LNKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFE 267

Query: 241 PDGLHLTTPSQVRLGGVLADAYRRFPSHPL-ATPLTNAAPISIFSTFFLSISWIFTFFLL 300
           PDGLHL TPSQV+LGGVLADAYRRFP HPL A+PL +AA  +   +FF+S+    TF L 
Sbjct: 268 PDGLHLNTPSQVKLGGVLADAYRRFPPHPLAASPLRSAASTASVHSFFISMFRTLTFVL- 327

Query: 301 FLHLILL 305
            LH ILL
Sbjct: 328 -LHQILL 332

BLAST of PI0004077 vs. NCBI nr
Match: XP_022982273.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 476.1 bits (1224), Expect = 2.3e-130
Identity = 245/307 (79.80%), Postives = 264/307 (85.99%), Query Frame = 0

Query: 1   MLYLCLLFLTTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPP 60
           MLY  LLFL  AA +P TSQQP PPT IFLLAGQSNMAGRGGV+N+TLTH  TWDGVVPP
Sbjct: 1   MLYFSLLFL-MAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVSNTTLTHRPTWDGVVPP 60

Query: 61  QCSPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLV 120
           QCSP P ILRLA+DLTWVEAREPLHADIDFLK NGIGPGMPFANTILMDKP GRTVIGLV
Sbjct: 61  QCSPNPSILRLASDLTWVEAREPLHADIDFLKINGIGPGMPFANTILMDKPAGRTVIGLV 120

Query: 121 PCAIGGTSIKEWQQGSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGR 180
           PCA+GGTSI++WQ+GSNLYNHLL+RA+AS+L GG IKALLWYQGESDT NAEDSELYGGR
Sbjct: 121 PCAMGGTSIQQWQKGSNLYNHLLTRAEASILGGGTIKALLWYQGESDTVNAEDSELYGGR 180

Query: 181 LKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVD--ALGLSLE 240
           LKKFFTDIRSDL IP LPIIQVGIASGEG YKEGVR+GQFGI+++NVM VD  ALGLS E
Sbjct: 181 LKKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQFGIEVMNVMTVDAYALGLSFE 240

Query: 241 PDGLHLTTPSQVRLGGVLADAYRRFPSHPL-ATPLTNAAPISIFSTFFLSISWIFTFFLL 300
           PDGLHL TPSQV+LGGVLADAYRRFP HPL A+PL NAA  S  S +   IS   T   +
Sbjct: 241 PDGLHLNTPSQVKLGGVLADAYRRFPPHPLAASPLRNAA--STASVYSFCISMFRTMTFV 300

Query: 301 FLHLILL 305
           FLH ILL
Sbjct: 301 FLHQILL 304

BLAST of PI0004077 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 282.3 bits (721), Expect = 4.5e-76
Identity = 148/295 (50.17%), Postives = 198/295 (67.12%), Query Frame = 0

Query: 3   YLCLLFLTTAAQIPATSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPPQC 62
           +  ++FL +   +   SQ  +    IF+LAGQSNMAGRGGV N T T+ T WDGV+PP+C
Sbjct: 8   FFIIIFLQSPPHL--QSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPEC 67

Query: 63  SPTPYILRLAADLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLVPC 122
              P ILRL + L W EA+EPLH DID  KTNG+GPGMPFAN + +++ G    +GLVPC
Sbjct: 68  RSNPSILRLTSKLEWKEAKEPLHVDIDINKTNGVGPGMPFANRV-VNRFG---QVGLVPC 127

Query: 123 AIGGTSIKEWQQGSNLYNHLLSRADASVLS--GGKIKALLWYQGESDTENAEDSELYGGR 182
           +IGGT + +WQ+G  LY   + RA A++ S  GG  +A+LWYQGESDT +  D+ +Y  R
Sbjct: 128 SIGGTKLSQWQKGEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKR 187

Query: 183 LKKFFTDIRSDLKIPLLPIIQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEPD 242
           L KFF+D+R+DL+ P LPIIQV +A+G G Y + VRK Q   DL NV  VDA GL LEPD
Sbjct: 188 LVKFFSDLRNDLQHPNLPIIQVALATGAGPYLDAVRKAQLKTDLENVYCVDARGLPLEPD 247

Query: 243 GLHLTTPSQVRLGGVLADAYRRFPSHPLATPLTNAAPISIFSTFFLSISWIFTFF 296
           GLHLTT SQV+LG ++A+++   P+   A P       S+F + ++S++   T F
Sbjct: 248 GLHLTTSSQVQLGHMIAESFLAIPNS--ARPSLYMHFFSLFCSLYVSLNIFGTIF 294

BLAST of PI0004077 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 268.1 bits (684), Expect = 8.8e-72
Identity = 131/241 (54.36%), Postives = 164/241 (68.05%), Query Frame = 0

Query: 20  QQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPPQCSPTPYILRLAADLTWVE 79
           Q P PP  IF+L+GQSNMAGRGGV      +   WD ++PP+C+P   ILRL+ADL W E
Sbjct: 16  QSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEE 75

Query: 80  AREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLVPCAIGGTSIKEWQQGSNLY 139
           A EPLH DID  K  G+GPGM FAN +         VIGLVPCA GGT+IKEW++GS+LY
Sbjct: 76  AHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLY 135

Query: 140 NHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGRLKKFFTDIRSDLKIPLLPI 199
             ++ R + S   GG+IKA+LWYQGESD  +  D+E YG  + +   ++R DL +P LPI
Sbjct: 136 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 195

Query: 200 IQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEPDGLHLTTPSQVRLGGVLADA 259
           IQV IASG G Y + VR+ Q G+ L NV+ VDA GL L+ D LHLTT +QV+LG  LA A
Sbjct: 196 IQVAIASG-GGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQA 255

Query: 260 Y 261
           Y
Sbjct: 256 Y 255

BLAST of PI0004077 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 268.1 bits (684), Expect = 8.8e-72
Identity = 131/241 (54.36%), Postives = 164/241 (68.05%), Query Frame = 0

Query: 20  QQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHTTWDGVVPPQCSPTPYILRLAADLTWVE 79
           Q P PP  IF+L+GQSNMAGRGGV      +   WD ++PP+C+P   ILRL+ADL W E
Sbjct: 16  QSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEE 75

Query: 80  AREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLVPCAIGGTSIKEWQQGSNLY 139
           A EPLH DID  K  G+GPGM FAN +         VIGLVPCA GGT+IKEW++GS+LY
Sbjct: 76  AHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLY 135

Query: 140 NHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGRLKKFFTDIRSDLKIPLLPI 199
             ++ R + S   GG+IKA+LWYQGESD  +  D+E YG  + +   ++R DL +P LPI
Sbjct: 136 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 195

Query: 200 IQVGIASGEGEYKEGVRKGQFGIDLVNVMIVDALGLSLEPDGLHLTTPSQVRLGGVLADA 259
           IQV IASG G Y + VR+ Q G+ L NV+ VDA GL L+ D LHLTT +QV+LG  LA A
Sbjct: 196 IQVAIASG-GGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQA 255

Query: 260 Y 261
           Y
Sbjct: 256 Y 255