PI0003934 (gene) Melon (PI 482460) v1

Overview
NamePI0003934
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionSerine hydroxymethyltransferase
Locationchr05: 16089500 .. 16091914 (-)
RNA-Seq ExpressionPI0003934
SyntenyPI0003934
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCCGTCAATGATTGGGGTAACACTCCTCTCAACACCGTCGACCCTGAGATTTTCGATCTCATCGAGAAGGAGAAGCGTCGACAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACTTCCTTCGCCGTCATCGAAGCCCTTGGCAGTGCCTTGACCAACAAGTACTCCGAAGGCATGCCGGGAAATCGATACTATGGTGGAAATGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCCAGAGCTCTCCAGGCCTTCCATTGCGATCCGGCGAAATGGGGCGTTAATGTCCAGCCTTACTCTGGTTCACCCGCGAATTTCGCCGCTTACACTGCGTTGCTTCAGCCACACGACCGGATTATGGGACTGGATCTTCCATCTGGTGGTCATTTGACGCATGGTTATTACACCTCTGGTGGAAAGAAGATCTCTGCTACCTCCATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCACTACTGGTTATATTGATTACGTTAAGTTGGAGGAGAAGGCTTTGGATTTCAGACCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCAATTGCTGATAAATGCGGTGCTCTTCTTCTCTGTGATATGGCTCACATAAGTGGACTGGTTGCTGCTCAGGTATCATAAACGAAAGAAATCTATGAATTTAGTTCAGATCTTATGTTATTTTTTTCCTGCAAATGTTGGCACTTGGATGTTGTTTTACATTTCTGGATTTGGGATCTGTTGGTTTTTGTTGAATTTGTTATTCTCTTTGATGTCTTCAGATGATATGCTTGATTTGTTCATACTTTTCATTCGTATCTGTGTTCAGTGTTCACTGAACTGATTAGGAATGGTTAGAAATGACATAGGTCTTTAGATCCATAACTGTTTTGAGCATCTGACGTAACAGAATCTAGGTTTTTAACAAACTTATTTAATGATTAATTATCTATGCTACATTGCTTCTTTTTAACGAATTATGTTCTTTGGATAAATGGAACCATTGTGTTTTTCATTGCTTCCGACAAATATTTTGCATTCTTTGTCTTTAAGATTCAGTTTTAAATGCTAGATTAAAATTGAAATGAAGATCTACTTAGAGGAAGCGGATACATTCCTGCTTCTTGTAGAATTAGTTTCAATGTAAAACAAATCCTGGGTCCAATGATCTTTTTGTTTGGACTTACATTACGTTCCTTGTGAATTGAGAAACCCTATGGCTCGAAAAAAGCTTTAGTATTGTAAAATATTAATGTCATATGATTTTGAAGGCCTATTTCTTCTAAGACATTATGATTGTTTATGATCTCTGTTTCTTTCCTTGCAACTTAAGCAATTTGGGTCTGGCTCGTATTGTATAATATTAAAATTCAATGATTGTTTGCAGGAAGCTGCAAACCCATTCGAGTATTGTGATGTCGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCGAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCACCTAAGAAGGGTCAGCCCGAAGATGCTGTTTATGACTATGAAGACAAGATCAACTTCTCCGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCTCCTGGGTTCAAGGCCTATGCTAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAATAAGGGTTACAAGCTTGTCACTGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGTAATTTTATGACGAATCAACTTATAGAAAACTAACATCAGTAGAAGTCTTAACAGTTTTTCTCGGCATCAGTCTTTTAAGTCATTGAGAAGCGGTCTTGTGGTAATTCATTTTGCAGGCAACAAGGTTGAGAAGCTCTGCGACTTGTGCAACATCACCGTAAACAAAAACGCAGTGTTTGGTGACAGCAGTGCATTGGCTCCCGGAGGTGTCCGAATTGGTAAGTACCGCGATATCATTGGAAGATGACTTCATTATCTACTAACTTGTTTTTCCTGCAATCAAACGAACAAGTAGAAATTTCAAAGTGTAATTAACAAGACATCAAATTATGTTGTTATTATTAGGTGCACCTGCCATGACTTCAAGAGGTTTGGTAGAGAAAGACTTCGAACAGATAGCAGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAACGTCCAGAAGGAATATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTTGTCAACAACAAGGAAATTGAAGAGCTCAAGGCCGATGTCGAGAAATTTTCCGGCTCTTTTGACATGCCAGGCTTCCTCATGTCGGAGATGAAGTACAAGGACTAG

mRNA sequence

ATGGATCCCGTCAATGATTGGGGTAACACTCCTCTCAACACCGTCGACCCTGAGATTTTCGATCTCATCGAGAAGGAGAAGCGTCGACAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACTTCCTTCGCCGTCATCGAAGCCCTTGGCAGTGCCTTGACCAACAAGTACTCCGAAGGCATGCCGGGAAATCGATACTATGGTGGAAATGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCCAGAGCTCTCCAGGCCTTCCATTGCGATCCGGCGAAATGGGGCGTTAATGTCCAGCCTTACTCTGGTTCACCCGCGAATTTCGCCGCTTACACTGCGTTGCTTCAGCCACACGACCGGATTATGGGACTGGATCTTCCATCTGGTGGTCATTTGACGCATGGTTATTACACCTCTGGTGGAAAGAAGATCTCTGCTACCTCCATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCACTACTGGTTATATTGATTACGTTAAGTTGGAGGAGAAGGCTTTGGATTTCAGACCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCAATTGCTGATAAATGCGGTGCTCTTCTTCTCTGTGATATGGCTCACATAAGTGGACTGGTTGCTGCTCAGGAAGCTGCAAACCCATTCGAGTATTGTGATGTCGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCGAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCACCTAAGAAGGGTCAGCCCGAAGATGCTGTTTATGACTATGAAGACAAGATCAACTTCTCCGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCTCCTGGGTTCAAGGCCTATGCTAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAATAAGGGTTACAAGCTTGTCACTGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGCAACAAGGTTGAGAAGCTCTGCGACTTGTGCAACATCACCGTAAACAAAAACGCAGTGTTTGGTGACAGCAGTGCATTGGCTCCCGGAGGTGTCCGAATTGGTGCACCTGCCATGACTTCAAGAGGTTTGGTAGAGAAAGACTTCGAACAGATAGCAGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAACGTCCAGAAGGAATATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTTGTCAACAACAAGGAAATTGAAGAGCTCAAGGCCGATGTCGAGAAATTTTCCGGCTCTTTTGACATGCCAGGCTTCCTCATGTCGGAGATGAAGTACAAGGACTAG

Coding sequence (CDS)

ATGGATCCCGTCAATGATTGGGGTAACACTCCTCTCAACACCGTCGACCCTGAGATTTTCGATCTCATCGAGAAGGAGAAGCGTCGACAATGCCGCGGAATCGAGCTCATTGCTTCTGAGAATTTCACTTCCTTCGCCGTCATCGAAGCCCTTGGCAGTGCCTTGACCAACAAGTACTCCGAAGGCATGCCGGGAAATCGATACTATGGTGGAAATGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCCAGAGCTCTCCAGGCCTTCCATTGCGATCCGGCGAAATGGGGCGTTAATGTCCAGCCTTACTCTGGTTCACCCGCGAATTTCGCCGCTTACACTGCGTTGCTTCAGCCACACGACCGGATTATGGGACTGGATCTTCCATCTGGTGGTCATTTGACGCATGGTTATTACACCTCTGGTGGAAAGAAGATCTCTGCTACCTCCATTTACTTCGAGAGTTTGCCTTACAAGGTTGATTCCACTACTGGTTATATTGATTACGTTAAGTTGGAGGAGAAGGCTTTGGATTTCAGACCGAAGTTGATTATCTGTGGTGGCAGTGCGTACCCGAGGGACTGGGATTATGCCAGATTCAGGGCAATTGCTGATAAATGCGGTGCTCTTCTTCTCTGTGATATGGCTCACATAAGTGGACTGGTTGCTGCTCAGGAAGCTGCAAACCCATTCGAGTATTGTGATGTCGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCGAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAGCCACCTAAGAAGGGTCAGCCCGAAGATGCTGTTTATGACTATGAAGACAAGATCAACTTCTCCGTCTTCCCAGCCCTTCAGGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCTCCTGGGTTCAAGGCCTATGCTAAACAAGTTAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAATAAGGGTTACAAGCTTGTCACTGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGCAACAAGGTTGAGAAGCTCTGCGACTTGTGCAACATCACCGTAAACAAAAACGCAGTGTTTGGTGACAGCAGTGCATTGGCTCCCGGAGGTGTCCGAATTGGTGCACCTGCCATGACTTCAAGAGGTTTGGTAGAGAAAGACTTCGAACAGATAGCAGAGTTCCTGCACCGTGCTGTAACCATCACCTTGAACGTCCAGAAGGAATATGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTTGTCAACAACAAGGAAATTGAAGAGCTCAAGGCCGATGTCGAGAAATTTTCCGGCTCTTTTGACATGCCAGGCTTCCTCATGTCGGAGATGAAGTACAAGGACTAG

Protein sequence

MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDFRPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Homology
BLAST of PI0003934 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 7.6e-261
Identity = 431/471 (91.51%), Postives = 455/471 (96.60%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV+ WGNT L +VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRAL+AFHCDPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+ TTGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFRAIADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYD+EDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFK YAKQVKANAVALGNYLM+KGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           ++QK YGKLLKDFNKGLVNNK++++LKADVEKFS S++MPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 1.1e-235
Identity = 383/468 (81.84%), Postives = 430/468 (91.88%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV  WGNT L+ VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+L+AFHC+P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TGYIDY KLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFRA+ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYD++ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FK YAKQVKANA  L +YL+NKGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMK 469
           ++Q++YGK++KDFNKGLVNNKEI+E+KADVE+F+  FDMPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

BLAST of PI0003934 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.0e-177
Identity = 294/466 (63.09%), Postives = 368/466 (78.97%), Query Frame = 0

Query: 4   VNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V  WG+ P++  DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189

Query: 64  PGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IENLC  RAL AF  +  KWGVNVQPYS + ANFA YT LL P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDFRPK 183
           IMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TGYIDY KLE+KALD+RPK
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPK 309

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDWD+AR R IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TTH
Sbjct: 310 ILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTH 369

Query: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEDAVYDYEDKINFSVFPALQGGPHNHQIGA 303
           K LRGPR G+IFYR+GPK  K+G           YD E+KINF+VFP+LQGGPHN+ I A
Sbjct: 370 KGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAA 429

Query: 304 LAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQ  +P +KAY +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG 
Sbjct: 430 LAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGK 489

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  I
Sbjct: 490 VYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

Query: 424 TLNVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLM 465
           T  +Q+E+GK  K+F K L  NK+I EL+  VE F+  ++MP  L+
Sbjct: 550 TSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

BLAST of PI0003934 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.3e-175
Identity = 294/466 (63.09%), Postives = 366/466 (78.54%), Query Frame = 0

Query: 4   VNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V  WGN  +   DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF  +  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TGYIDY KLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEDAVYDYEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         +  YD+E+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K Y +QVK NA AL + L+++  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLNVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLM 465
               Q+E+GKL K+  K + + KEI +L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of PI0003934 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 7.0e-174
Identity = 300/454 (66.08%), Postives = 362/454 (79.74%), Query Frame = 0

Query: 8   GNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNR 67
           GNTPL  VD EIF+L+ +EK RQ +G+ELIASENFTS AV+EALGS  TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 68  YYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGL 127
           YYGG E +DE+E LC+ RAL+AF  D +KWGVNVQPYSGSPANFA YTALL+PHDRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 128 DLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDFRPKLIIC 187
           DLPSGGHLTHGY T   KKISA+SI+FES+PY++    G IDY +LEE AL F+PKLII 
Sbjct: 126 DLPSGGHLTHGYQTD-KKKISASSIFFESMPYQI-GADGLIDYQRLEENALLFKPKLIIS 185

Query: 188 GGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLR 247
           G SAYPR+WDY R RAIADK GA L+CDMAH SGLVAAQ   +PF+YCDVVT+TTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 248 GPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALAVALKQAM 307
           GPR+G+IF+R+G +    G+ E   YD E KINF+VFP+LQGGPH + I  +AVALK+A 
Sbjct: 246 GPRSGIIFFRRGKRVDGNGK-EIEEYDIESKINFAVFPSLQGGPHENVIAGVAVALKEAD 305

Query: 308 SPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLC 367
           S  FK YA QVK NA A+GN LMNKGYKLVT GT+NHL+LWDLRP  LTGNK EK  D+ 
Sbjct: 306 SQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEKAADIA 365

Query: 368 NITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNVQKEYG 427
           NITVNKNAV GD++A++PGG+RIG+ A+TSRGL E DFE+IA+FL R V+I+L +Q   G
Sbjct: 366 NITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEIQGRVG 425

Query: 428 KLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPG 462
           K L DF   +  +KE+ +L+ +VE+FS  F +PG
Sbjct: 426 KKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

BLAST of PI0003934 vs. ExPASy TrEMBL
Match: A0A0A0LKC8 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.2e-278
Identity = 467/471 (99.15%), Postives = 469/471 (99.58%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           NVQKEYGKLLKDFNKGLVNNKEIE+LKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. ExPASy TrEMBL
Match: A0A5A7SVK0 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold275G00690 PE=3 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 5.4e-278
Identity = 467/471 (99.15%), Postives = 468/471 (99.36%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALQAF CDP KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFRCDPTKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. ExPASy TrEMBL
Match: A0A1S3C536 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=6F11 PE=3 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 5.4e-278
Identity = 467/471 (99.15%), Postives = 468/471 (99.36%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALQAF CDP KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFRCDPTKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. ExPASy TrEMBL
Match: A0A6J1CDF6 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.5e-272
Identity = 458/471 (97.24%), Postives = 463/471 (98.30%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVNAWGNTPLKAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHADPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKEYGKLLKDFNKGLVNNK+IE LKADVEKFSGSFDMPGF MSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKDIEGLKADVEKFSGSFDMPGFAMSEMKYKD 471

BLAST of PI0003934 vs. ExPASy TrEMBL
Match: A0A6J1KLX2 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111494472 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 4.5e-272
Identity = 456/471 (96.82%), Postives = 464/471 (98.51%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLEAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALQAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+S+TGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSSTGYIDYEKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYA+FRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYAKFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKVYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRA+TITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAITITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKEYGKLLKDFNKGLVNNKEIEELKADVEKFS SFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSASFDMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. NCBI nr
Match: XP_004138967.1 (serine hydroxymethyltransferase 4 [Cucumis sativus] >KGN61489.1 hypothetical protein Csa_006275 [Cucumis sativus])

HSP 1 Score: 966.5 bits (2497), Expect = 8.6e-278
Identity = 467/471 (99.15%), Postives = 469/471 (99.58%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           NVQKEYGKLLKDFNKGLVNNKEIE+LKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. NCBI nr
Match: XP_008457222.1 (PREDICTED: serine hydroxymethyltransferase 4 [Cucumis melo] >KAA0033337.1 serine hydroxymethyltransferase 4 [Cucumis melo var. makuwa] >TYK21570.1 serine hydroxymethyltransferase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 966.1 bits (2496), Expect = 1.1e-277
Identity = 467/471 (99.15%), Postives = 468/471 (99.36%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALQAF CDP KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFRCDPTKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. NCBI nr
Match: XP_038875387.1 (serine hydroxymethyltransferase 4 [Benincasa hispida])

HSP 1 Score: 954.5 bits (2466), Expect = 3.4e-274
Identity = 463/471 (98.30%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ VN WGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MESVNAWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALQAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIG PAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           NVQKE+GKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. NCBI nr
Match: XP_022138598.1 (serine hydroxymethyltransferase 4 [Momordica charantia])

HSP 1 Score: 948.0 bits (2449), Expect = 3.2e-272
Identity = 458/471 (97.24%), Postives = 463/471 (98.30%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVNAWGNTPLKAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHADPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKEYGKLLKDFNKGLVNNK+IE LKADVEKFSGSFDMPGF MSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKDIEGLKADVEKFSGSFDMPGFAMSEMKYKD 471

BLAST of PI0003934 vs. NCBI nr
Match: XP_022964401.1 (serine hydroxymethyltransferase 4 [Cucurbita moschata] >XP_023000183.1 serine hydroxymethyltransferase 4 [Cucurbita maxima] >XP_023514660.1 serine hydroxymethyltransferase 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 946.4 bits (2445), Expect = 9.2e-272
Identity = 456/471 (96.82%), Postives = 464/471 (98.51%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPVN WGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLEAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALQAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+S+TGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSSTGYIDYEKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYA+FRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYAKFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKVYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRA+TITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAITITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           N+QKEYGKLLKDFNKGLVNNKEIEELKADVEKFS SFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSASFDMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 900.6 bits (2326), Expect = 5.4e-262
Identity = 431/471 (91.51%), Postives = 455/471 (96.60%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV+ WGNT L +VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRAL+AFHCDPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+ TTGYIDY KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFRAIADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYD+EDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFK YAKQVKANAVALGNYLM+KGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           ++QK YGKLLKDFNKGLVNNK++++LKADVEKFS S++MPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

BLAST of PI0003934 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 817.0 bits (2109), Expect = 7.8e-237
Identity = 383/468 (81.84%), Postives = 430/468 (91.88%), Query Frame = 0

Query: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV  WGNT L+ VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+L+AFHC+P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TGYIDY KLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFRA+ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYD++ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FK YAKQVKANA  L +YL+NKGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 NVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMK 469
           ++Q++YGK++KDFNKGLVNNKEI+E+KADVE+F+  FDMPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

BLAST of PI0003934 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 624.4 bits (1609), Expect = 7.5e-179
Identity = 294/466 (63.09%), Postives = 368/466 (78.97%), Query Frame = 0

Query: 4   VNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V  WG+ P++  DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189

Query: 64  PGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IENLC  RAL AF  +  KWGVNVQPYS + ANFA YT LL P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDFRPK 183
           IMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TGYIDY KLE+KALD+RPK
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPK 309

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDWD+AR R IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TTH
Sbjct: 310 ILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTH 369

Query: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEDAVYDYEDKINFSVFPALQGGPHNHQIGA 303
           K LRGPR G+IFYR+GPK  K+G           YD E+KINF+VFP+LQGGPHN+ I A
Sbjct: 370 KGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAA 429

Query: 304 LAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQ  +P +KAY +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG 
Sbjct: 430 LAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGK 489

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  I
Sbjct: 490 VYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

Query: 424 TLNVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLM 465
           T  +Q+E+GK  K+F K L  NK+I EL+  VE F+  ++MP  L+
Sbjct: 550 TSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

BLAST of PI0003934 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 617.5 bits (1591), Expect = 9.1e-177
Identity = 294/466 (63.09%), Postives = 366/466 (78.54%), Query Frame = 0

Query: 4   VNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V  WGN  +   DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF  +  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TGYIDY KLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEDAVYDYEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         +  YD+E+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K Y +QVK NA AL + L+++  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLNVQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLM 465
               Q+E+GKL K+  K + + KEI +L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of PI0003934 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 558.9 bits (1439), Expect = 3.8e-159
Identity = 280/465 (60.22%), Postives = 343/465 (73.76%), Query Frame = 0

Query: 3   PVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEG 62
           P  D+G   L  VDPE+  +I KEK RQ R +ELIASENFTS AV+EA+GS LTNKYSEG
Sbjct: 77  PFEDYG---LGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEG 136

Query: 63  MPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQPHD 122
           +PG RYYGGNE+ID++E LC++RAL AF  D  KWGVNVQP SGSPANFA YTA+L PHD
Sbjct: 137 LPGKRYYGGNEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPANFAVYTAILSPHD 196

Query: 123 RIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYVKLEEKALDFRP 182
           RIMGLDLP GGHL+HG+ T+  +++S TSIYFES+PY++D +TG +DY  LE+ A  FRP
Sbjct: 197 RIMGLDLPHGGHLSHGFMTA-KRRVSGTSIYFESMPYRLDESTGIVDYDMLEKTATLFRP 256

Query: 183 KLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTT 242
           KLII G SAY RD+DY R R IAD  GA L+ DMAHISGLVAA   A+PFEYCD+VTTTT
Sbjct: 257 KLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTT 316

Query: 243 HKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALAVA 302
           HKSLRGPR GMIF+RK P             D E  +N +VFP LQGGPHNH IG LAV 
Sbjct: 317 HKSLRGPRGGMIFFRKDPING---------VDLESAVNNAVFPGLQGGPHNHTIGGLAVC 376

Query: 303 LKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKVEK 362
           LK A SP FKAY K+V +N  AL N L+  G+KLV+GG++NHLVL DLRP+G+ G +VEK
Sbjct: 377 LKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEK 436

Query: 363 LCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLNV 422
           + D+ +IT+NKN+V GD SAL PGG+RIG+PAMT+RGL EKDF  +A+F+   V IT+  
Sbjct: 437 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEA 496

Query: 423 QKEY-GKLLKDFNKGLVN-----NKEIEELKADVEKFSGSFDMPG 462
           +K   G  L+DFNK + +      + ++ LK  VE F+  F +PG
Sbjct: 497 KKAAPGSKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O232547.6e-26191.51Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S... [more]
Q9SVM41.1e-23581.84Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S... [more]
Q84WV01.0e-17763.09Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S... [more]
Q9LM591.3e-17563.09Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S... [more]
Q54Z267.0e-17466.08Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LKC84.2e-27899.15Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 ... [more]
A0A5A7SVK05.4e-27899.15Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C5365.4e-27899.15Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=6F11 PE=3 SV=1[more]
A0A6J1CDF61.5e-27297.24Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 P... [more]
A0A6J1KLX24.5e-27296.82Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111494472 PE=3... [more]
Match NameE-valueIdentityDescription
XP_004138967.18.6e-27899.15serine hydroxymethyltransferase 4 [Cucumis sativus] >KGN61489.1 hypothetical pro... [more]
XP_008457222.11.1e-27799.15PREDICTED: serine hydroxymethyltransferase 4 [Cucumis melo] >KAA0033337.1 serine... [more]
XP_038875387.13.4e-27498.30serine hydroxymethyltransferase 4 [Benincasa hispida][more]
XP_022138598.13.2e-27297.24serine hydroxymethyltransferase 4 [Momordica charantia][more]
XP_022964401.19.2e-27296.82serine hydroxymethyltransferase 4 [Cucurbita moschata] >XP_023000183.1 serine hy... [more]
Match NameE-valueIdentityDescription
AT4G13930.15.4e-26291.51serine hydroxymethyltransferase 4 [more]
AT4G13890.17.8e-23781.84Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT1G36370.17.5e-17963.09serine hydroxymethyltransferase 7 [more]
AT1G22020.19.1e-17763.09serine hydroxymethyltransferase 6 [more]
AT4G32520.13.8e-15960.22serine hydroxymethyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 16..460
e-value: 7.9E-214
score: 712.0
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 41..316
e-value: 7.9E-214
score: 712.0
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 12..411
e-value: 5.0E-191
score: 634.5
IPR001085Serine hydroxymethyltransferasePIRSFPIRSF000412SHMTcoord: 1..466
e-value: 4.1E-189
score: 624.2
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 1..470
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 11..461
score: 42.502769
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 15..453
e-value: 0.0
score: 617.677
NoneNo IPR availablePANTHERPTHR11680:SF33SERINE HYDROXYMETHYLTRANSFERASEcoord: 1..470
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 236..252
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 7..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003934.1PI0003934.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity