PI0003812 (gene) Melon (PI 482460) v1

BLAST of PI0003812 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.0e-45
Identity = 219/909 (24.09%), Postives = 407/909 (44.77%), Query Frame = 0

Query: 19  PILDELSCLFHYN-----ENIEKFEEKLKIL-ETTEDRVQIFVSQERDEGNAIFDD---- 78
           P++ E+  L + +      N  KF+  +K L E+ E   ++  +   D    +  D    
Sbjct: 6   PVIGEILRLMYESTFSRVANAIKFKSNVKALNESLERLTELKGNMSEDHETLLTKDKPLR 65

Query: 79  --VTKWLAKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKI-------IELID- 138
             + +W  + +E +  A       +SC     R ++SRK  K L+++       IE +D 
Sbjct: 66  LKLMRWQREAEEVISKARLKLEERVSC-GMSLRPRMSRKLVKILDEVKMLEKDGIEFVDM 125

Query: 139 -------------EGKRFDHGSNIGYHLPFLPQTTSSTRPEDYQIF----ESRTSLCEKL 198
                         G    H +     L  +    +S + +   ++      +T+L   L
Sbjct: 126 LSVESTPERVEHVPGVSVVHQTMASNMLAKIRDGLTSEKAQKIGVWGMGGVGKTTLVRTL 185

Query: 199 RMHLQNLMSTRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEI--RVELNKKLEQGEKVR 258
              L+   +T+          F  VI V V +     ++QK+I  R++++ ++E+ E+  
Sbjct: 186 NNKLREEGATQ---------PFGLVIFVIVSKEFDPREVQKQIAERLDIDTQMEESEEKL 245

Query: 259 SSRGQTHVIVEKKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFT 318
           + R   +V + K++  L ILDD+WK  D++    IP   E +     K+++  R  ++  
Sbjct: 246 ARR--IYVGLMKERKFLLILDDVWKPIDLD-LLGIPRTEENK---GSKVILTSRFLEV-C 305

Query: 319 NHMDIKELIEVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIAT 378
             M     + V+CL EE++W  F    GD +V  + ++++ K V +ECGGLP+A+  + T
Sbjct: 306 RSMKTDLDVRVDCLLEEDAWELFCKNAGD-VVRSDHVRKIAKAVSQECGGLPLAIITVGT 365

Query: 379 ILKGKR-VEIWKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIEDEAQLLLLLCSVFPD 438
            ++GK+ V++W   L+KL   V   I  + ++++  L+ SY+ +ED+A+   LLC++FP+
Sbjct: 366 AMRGKKNVKLWNHVLSKLSKSVPW-IKSIEEKIFQPLKLSYDFLEDKAKFCFLLCALFPE 425

Query: 439 DALINVKDLQMYAMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLLQ-GDHDYFVQMHDVV 498
           D  I V ++  Y M  G +  + +  D+ N  I  V+ LK   LL+ GD    V+MHDVV
Sbjct: 426 DYSIEVTEVVRYWMAEGFMEELGSQEDSMNEGITTVESLKDYCLLEDGDRRDTVKMHDVV 485

Query: 499 HDFAKY--TASKKDKMTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQ 558
            DFA +  ++S+ D  + +   +G  +  ++    S   +        +LP  +E    +
Sbjct: 486 RDFAIWIMSSSQDDSHSLVMSGTGLQDIRQDKLAPSLRRVSLMNNKLESLPDLVE----E 545

Query: 559 LFILINIGSLETDFQISDT---FFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCL 618
             +  ++  L+ +F + +    F +    L++L LS          SL   L +L +  L
Sbjct: 546 FCVKTSVLLLQGNFLLKEVPIGFLQAFPTLRILNLSGTRIKSFPSCSLLR-LFSLHSLFL 605

Query: 619 SNCY-FVDIDTIGKLKKLKILKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVIHPDII 678
            +C+  V + ++  L KL++L +    I E P  + EL + + L +S   +L+ I   ++
Sbjct: 606 RDCFKLVKLPSLETLAKLELLDLCGTHILEFPRGLEELKRFRHLDLSRTLHLESIPARVV 665

Query: 679 SKLTRLEELCFHENEFEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNE 738
           S+L+ LE L    + +    +  + K +    A + E+ CL+ L  L++R+ +   L N+
Sbjct: 666 SRLSSLETLDMTSSHYRWSVQGETQKGQ----ATVEEIGCLQRLQVLSIRLHSSPFLLNK 725

Query: 739 LSLETTRNLKEFNI---STDWIWERGSSNAILKLAIKSPIDSIGGVL----QILLQKCKY 798
            +    R LK+F +   S   +  R     +    +     SIG +L     + L  C+ 
Sbjct: 726 RNTWIKR-LKKFQLVVGSRYILRTRHDKRRLTISHLNVSQVSIGWLLAYTTSLALNHCQG 785

Query: 799 L------TLDDSVGFTKLFLNPHENYIYPRVENLKLYIRRRIGNLSPHNFNLQVAFPRLE 858
           +       + D+ GF  L     EN I     N   ++     N S  + ++    P LE
Sbjct: 786 IEAMMKKLVSDNKGFKNLKSLTIENVII----NTNSWVEMVSTNTSKQSSDILDLLPNLE 845

Query: 859 TLHIFGGNHFETLWHNNVQTPNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQ 868
            LH+   +  ET           L  LK +EI  C KL  +       +  +L  + I  
Sbjct: 846 ELHLRRVD-LETFSELQTHLGLKLETLKIIEITMCRKLRTLLDKRNFLTIPNLEEIEISY 880

BLAST of PI0003812 vs. ExPASy Swiss-Prot
Match: O82484 (Putative disease resistance protein At4g10780 OS=Arabidopsis thaliana OX=3702 GN=At4g10780 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 5.9e-29
Identity = 144/529 (27.22%), Postives = 236/529 (44.61%), Query Frame = 0

Query: 180 KNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVI-VEKKKNILFI 239
           KN  D VI V V     + +IQ++I  +L    ++  K + S+    ++    KK  + +
Sbjct: 201 KNGVDIVIWVVVSSDLQIHKIQEDIGEKLGFIGKEWNKKQESQKAVDILNCLSKKRFVLL 260

Query: 240 LDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEES 299
           LDD+WKK D+  +  IP  S+ R E  CK++   R  D+    M + + +EV CL+  ++
Sbjct: 261 LDDIWKKVDL-TKIGIP--SQTR-ENKCKVVFTTRSLDVCA-RMGVHDPMEVQCLSTNDA 320

Query: 300 WSFFVTIVGD-QLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKR-VEIWKDALNKL 359
           W  F   VG   L    DI ++ K V  +C GLP+AL +I   + GKR V+ W  A++ L
Sbjct: 321 WELFQEKVGQISLGSHPDILELAKKVAGKCRGLPLALNVIGETMAGKRAVQEWHHAVDVL 380

Query: 360 KNPVVVDIPGVSDQLYSCLQFSYNSIEDE-AQLLLLLCSVFPDDALINVKDLQMYAMGMG 419
            +    +  G+ D +   L++SY+++ D+  +     C+++P+D  I    L  Y +  G
Sbjct: 381 TS-YAAEFSGMDDHILLILKYSYDNLNDKHVRSCFQYCALYPEDYSIKKYRLIDYWICEG 440

Query: 420 LLNHVNTWNDARNRVIKLVDDLKSSYLL--QGDHDYFVQMHDVVHDFAKYTAS---KKDK 479
            ++       A N+  +++  L  + LL  +G +   V+MHDVV + A +T S   K  +
Sbjct: 441 FIDGNIGKERAVNQGYEILGTLVRACLLSEEGKNKLEVKMHDVVREMALWTLSDLGKNKE 500

Query: 480 MTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLETDFQ 539
              +   SG  +  + +  G+   +         +    E P L    L    SL     
Sbjct: 501 RCIVQAGSGLRKVPKVEDWGAVRRLSLMNNGIEEISGSPECPELTTLFLQENKSL---VH 560

Query: 540 ISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLK 599
           IS  FF  M KL VL LS+            H L  L             + I +L  L+
Sbjct: 561 ISGEFFRHMRKLVVLDLSE-----------NHQLDGLP------------EQISELVALR 620

Query: 600 ILKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHENEFEQW 659
            L +S   IE LP C+++L  L  L++     L  I    ISKL+ L  L    +     
Sbjct: 621 YLDLSHTNIEGLPACLQDLKTLIHLNLECMRRLGSIAG--ISKLSSLRTLGLRNS----- 680

Query: 660 GEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNELSLETTRN 700
                  N ++    + EL+ L+HL  LT+ I +  +L   +   T  N
Sbjct: 681 -------NIMLDVMSVKELHLLEHLEILTIDIVSTMVLEQMIDAGTLMN 683

BLAST of PI0003812 vs. ExPASy Swiss-Prot
Match: O64973 (Disease resistance protein RPS5 OS=Arabidopsis thaliana OX=3702 GN=RPS5 PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 2.8e-26
Identity = 168/694 (24.21%), Postives = 302/694 (43.52%), Query Frame = 0

Query: 183 FDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVIVE-----KKKNILF 242
           FD VI V V +S +V +IQ++I     +K+  G    S +    + V+     +++  + 
Sbjct: 207 FDVVIWVVVSRSSTVRKIQRDIA----EKVGLGGMEWSEKNDNQIAVDIHNVLRRRKFVL 266

Query: 243 ILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEE 302
           +LDD+W+K +++    +P  S+   +  CK+    R RD+    M + + +EV+CL  EE
Sbjct: 267 LLDDIWEKVNLK-AVGVPYPSK---DNGCKVAFTTRSRDV-CGRMGVDDPMEVSCLQPEE 326

Query: 303 SWSFFVTIVG-DQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKR-VEIWKDALNK 362
           SW  F   VG + L    DI  + + V ++C GLP+AL +I   +  KR V  W  A++ 
Sbjct: 327 SWDLFQMKVGKNTLGSHPDIPGLARKVARKCRGLPLALNVIGEAMACKRTVHEWCHAIDV 386

Query: 363 LKNPVVVDIPGVSDQLYSCLQFSYNSIEDE-AQLLLLLCSVFPDDALINVKDLQMYAMGM 422
           L +   +D  G+ D++   L++SY+++  E  +   L CS+FP+D LI+ + L  Y +  
Sbjct: 387 LTSS-AIDFSGMEDEILHVLKYSYDNLNGELMKSCFLYCSLFPEDYLIDKEGLVDYWISE 446

Query: 423 GLLNHVNTWNDARNRVIKLVDDLKSSYLL--QGDHDYFVQMHDVVHDFAKYTASKKDKMT 482
           G +N         N+  +++  L  + LL  +  +   V+MHDVV + A + +S   K  
Sbjct: 447 GFINEKEGRERNINQGYEIIGTLVRACLLLEEERNKSNVKMHDVVREMALWISSDLGKQK 506

Query: 483 SLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLETD-FQI 542
                       E  +   +N + +   + +N   +  F + +   L  +   + D  +I
Sbjct: 507 EKCIVRAGVGLREVPKVKDWNTVRK--ISLMNNEIEEIFDSHECAALTTLFLQKNDVVKI 566

Query: 543 SDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKI 602
           S  FF  M  L VL LS+             SL  L             + I +L  L+ 
Sbjct: 567 SAEFFRCMPHLVVLDLSE-----------NQSLNELP------------EEISELASLRY 626

Query: 603 LKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHENEFEQWG 662
             +S   I +LPV +  L +L  L++    +L  I    IS L  L  L   ++      
Sbjct: 627 FNLSYTCIHQLPVGLWTLKKLIHLNLEHMSSLGSILG--ISNLWNLRTLGLRDSRL---- 686

Query: 663 EEVSYKNKLITNAKLSELNCLKHLSCLTLRIQN----VEILSNELSLETTRN-----LKE 722
                   L+  + + EL  L+HL  +TL I +      +L ++  +E  +      LKE
Sbjct: 687 --------LLDMSLVKELQLLEHLEVITLDISSSLVAEPLLCSQRLVECIKEVDFKYLKE 746

Query: 723 FNISTDWIWERGSSNAILKLAIKSPIDSIGGVLQILLQKCKYLTLDDSVGFTKLFLNPHE 782
            ++    +   G+   + KL IK       G+ +I +++    +  +    T  F N   
Sbjct: 747 ESVRVLTLPTMGN---LRKLGIKR-----CGMREIKIERTTSSSSRNKSPTTPCFSNLSR 806

Query: 783 NYI--------------YPRVENLKLYIRRRIGNL------SPHNFNLQVAFPRLETLHI 837
            +I               P +  L++   + + ++        H+  + V F +LETLH+
Sbjct: 807 VFIAKCHGLKDLTWLLFAPNLTFLEVGFSKEVEDIISEEKAEEHSATI-VPFRKLETLHL 838

BLAST of PI0003812 vs. ExPASy Swiss-Prot
Match: Q42484 (Disease resistance protein RPS2 OS=Arabidopsis thaliana OX=3702 GN=RPS2 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.2e-25
Identity = 202/889 (22.72%), Postives = 368/889 (41.39%), Query Frame = 0

Query: 34  IEKFEEKLKILETTEDRVQIFVSQERDEGNAIFDDVTKWLAKVK-------------EEM 93
           I   E  +  L+   D + + + Q+  EG +  +   +WL+ V+                
Sbjct: 35  ITDLETAIGDLKAIRDDLTLRIQQDGLEGRSCSNRAREWLSAVQVTETKTALLLVRFRRR 94

Query: 94  DMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELID--EGKRFDHGS--NIGYHLPFL 153
           +     +   +SCF C   +++ +K    L+ I EL +  E  + D GS       +P  
Sbjct: 95  EQRTRMRRRYLSCFGCA-DYKLCKKVSAILKSIGELRERSEAIKTDGGSIQVTCREIPIK 154

Query: 154 PQTTSSTRPEDYQIFESRTSLCEKLRMH------LQNLMSTRLEYVKLEKNLFDWVIVVE 213
               ++T  E    F S       + ++         LM +    +  + + +D +I V+
Sbjct: 155 SVVGNTTMMEQVLEFLSEEEERGIIGVYGPGGVGKTTLMQSINNELITKGHQYDVLIWVQ 214

Query: 214 VGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVIVEKKKNILFILDDLWKKYDVEN 273
           + +      IQ+ +   L    ++ E    +R        ++K  L +LDD+W++ D+E 
Sbjct: 215 MSREFGECTIQQAVGARLGLSWDEKE-TGENRALKIYRALRQKRFLLLLDDVWEEIDLEK 274

Query: 274 EFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEESWSFFVTIV-GDQ 333
             V         E  CK++   R   L  N+M  +  + V  L ++ +W  F + V    
Sbjct: 275 TGV----PRPDRENKCKVMFTTRSIAL-CNNMGAEYKLRVEFLEKKHAWELFCSKVWRKD 334

Query: 334 LVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKRVEIWKDALNKLKNPVVVDIPGVSD 393
           L+E   I+++ + +V +CGGLP+AL  +   +  +  E      +++      ++ G+ +
Sbjct: 335 LLESSSIRRLAEIIVSKCGGLPLALITLGGAMAHRETEEEWIHASEVLTRFPAEMKGM-N 394

Query: 394 QLYSCLQFSYNSIE-DEAQLLLLLCSVFPDDALINVKDLQMYAMGMGLLNHVNTWNDARN 453
            +++ L+FSY+++E D  +   L C++FP++  I ++ L  Y +G G L    T +   N
Sbjct: 395 YVFALLKFSYDNLESDLLRSCFLYCALFPEEHSIEIEQLVEYWVGEGFL----TSSHGVN 454

Query: 454 RVIK---LVDDLKSSYLLQ-GDHDYFVQMHDVVHDFAKYTASKKDKMTSLTY---KSGQS 513
            + K   L+ DLK++ LL+ GD    V+MH+VV  FA + AS++     L       G +
Sbjct: 455 TIYKGYFLIGDLKAACLLETGDEKTQVKMHNVVRSFALWMASEQGTYKELILVEPSMGHT 514

Query: 514 EWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLETDFQISDTFFEGMEK 573
           E  + +       I         LP KL  P L   +L    SL+   +I   FF  M  
Sbjct: 515 EAPKAENWRQALVISLLDNRIQTLPEKLICPKLTTLMLQQNSSLK---KIPTGFFMHMPV 574

Query: 574 LQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKILKISSCEIEE 633
           L+VL L        S+TS+T          LS  Y V+         L  L +S  +I  
Sbjct: 575 LRVLDL--------SFTSITE-------IPLSIKYLVE---------LYHLSMSGTKISV 634

Query: 634 LPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHEN----EFEQWGEEVSYK 693
           LP  +  L +LK L +     L+ I  D I  L++LE L  + +    E + +GE+ +  
Sbjct: 635 LPQELGNLRKLKHLDLQRTQFLQTIPRDAICWLSKLEVLNLYYSYAGWELQSFGEDEA-- 694

Query: 694 NKLITNAKLSELNCLKHLSCLTLRIQNVEILS------------NELSLETTRNLKEFNI 753
                    ++L  L++L+ L + + ++E L               L +E    L  FN+
Sbjct: 695 ----EELGFADLEYLENLTTLGITVLSLETLKTLFEFGALHKHIQHLHVEECNELLYFNL 754

Query: 754 STDWIWERGSSNAILKLAIKSPID-----SIGGVLQILLQKCKYLTLDDSVGFTKLFLNP 813
            +  +   G +  + +L+IKS  D     +        L   + LTL      T+++ N 
Sbjct: 755 PS--LTNHGRN--LRRLSIKSCHDLEYLVTPADFENDWLPSLEVLTLHSLHNLTRVWGNS 814

Query: 814 HENYIYPRVENLKLYIRRRIGNLSPHNFNLQVAFPRLETLHIFGGNHFETLWHN----NV 866
                   +  + +    ++ N+S          P+LE + +F     E L       +V
Sbjct: 815 VSQDCLRNIRCINISHCNKLKNVS-----WVQKLPKLEVIELFDCREIEELISEHESPSV 867

BLAST of PI0003812 vs. ExPASy Swiss-Prot
Match: Q940K0 (Disease resistance protein UNI OS=Arabidopsis thaliana OX=3702 GN=UNI PE=1 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 4.9e-23
Identity = 144/621 (23.19%), Postives = 267/621 (43.00%), Query Frame = 0

Query: 32  ENIEKFEEKLKILETTEDRVQIFVSQERDEGNAIFDDVTKWLAKV-------KEEMDMAN 91
           +N+   + +++ L   +  VQ  V+++        + V  WL +V       K+ + +  
Sbjct: 32  KNLRALQREMEDLRAIQHEVQNKVARDEARHQRRLEAVQVWLDRVNSVDIECKDLLSVTP 91

Query: 92  ENKTPNISCFNCVR----RHQISRKAKKRLEKIIELIDEGKRFDHGSNIGYHLPFLPQTT 151
                   C  C +     ++  +K    LE++ +L  EG  FD  S          + T
Sbjct: 92  VELQKLCLCGLCSKYVCSSYKYGKKVFLLLEEVKKLNSEG-NFDEVSQPPPRSEVEERPT 151

Query: 152 SSTRPEDYQIFESRTSLCEK----LRMHLQNLMSTRLEYVKLEKNL------FDWVIVVE 211
             T  ++  + ++   L E     + +H    +     + K+          FD VI + 
Sbjct: 152 QPTIGQEDMLEKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAEIGGTFDIVIWIV 211

Query: 212 VGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHV-IVEKKKNILFILDDLWKKYDVE 271
           V +   + ++Q++I  +L+   +  +    S   T +  V K K  + +LDD+W+K D+E
Sbjct: 212 VSKGVMISKLQEDIAEKLHLCDDLWKNKNESDKATDIHRVLKGKRFVLMLDDIWEKVDLE 271

Query: 272 NEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEESWSFFVTIVGDQ 331
               IP  SE+     CK+    R R++     D K + +VNCL  E++W  F   VGD 
Sbjct: 272 -AIGIPYPSEV---NKCKVAFTTRSREVCGEMGDHKPM-QVNCLEPEDAWELFKNKVGDN 331

Query: 332 -LVEDEDIQQVGKHVVKECGGLPIALKIIATILKGK-RVEIWKDALNKLKNPVVVDIPGV 391
            L  D  I ++ + V ++C GLP+AL +I   +  K  V+ W+ A++ + N    +   +
Sbjct: 332 TLSSDPVIVELAREVAQKCRGLPLALNVIGETMSSKTMVQEWEHAIH-VFNTSAAEFSDM 391

Query: 392 SDQLYSCLQFSYNSIEDE-AQLLLLLCSVFPDDALINVKDLQMYAMGMGLLNHVNTWNDA 451
            +++   L++SY+S+ DE  +   L C++FP+D  I  + L  Y +  G +        A
Sbjct: 392 QNKILPILKYSYDSLGDEHIKSCFLYCALFPEDGEIYNEKLIDYWICEGFIGEDQVIKRA 451

Query: 452 RNRVIKLVDDLKSSYLLQGDHDYFVQMHDVVHDFAKYTASKKDKMTSLTYKSGQSEWEEE 511
           RN+   ++  L  + LL     Y+  MHDVV + A + AS   K         Q E    
Sbjct: 452 RNKGYAMLGTLTRANLLTKVGTYYCVMHDVVREMALWIASDFGK---------QKE---- 511

Query: 512 DRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILI--NIGSLETDFQISD--TFFEGMEKL 571
                 N + Q       +P   ++  ++   L+  +I  +  + + S+  T F    KL
Sbjct: 512 ------NFVVQAGVGLHEIPKVKDWGAVRKMSLMDNDIEEITCESKCSELTTLFLQSNKL 571

Query: 572 QVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKILKISSCEIEEL 624
           + L        P ++      L  L     +  +    + I  L  L+ L +S+  IE +
Sbjct: 572 KNL--------PGAFIRYMQKLVVLD-LSYNRDFNKLPEQISGLVSLQFLDLSNTSIEHM 617

BLAST of PI0003812 vs. ExPASy TrEMBL
Match: A0A6J1CPP4 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111013433 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 7.8e-274
Identity = 595/1251 (47.56%), Postives = 764/1251 (61.07%), Query Frame = 0

Query: 1    MDTVIGGVMGGVVEYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERD 60
            MD  I  V   VV+Y V PI  +L+ LF YN NI+  E++L+ L+ T +RVQ+ V++ R 
Sbjct: 1    MDVFI-SVGAKVVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARS 60

Query: 61   EGNAIFDDVTKWLAKVKEEMDMAN---------ENKTPNISCFNCVRRHQISRKAKKRLE 120
                I+D+V+ WL  V+ ++++ N            + N+SC N V+RH++SRK+KKR++
Sbjct: 61   NAYEIYDEVSTWLL-VEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVK 120

Query: 121  KIIELIDEGKRFDHGSNIGYHLPFLPQTTSSTRPEDYQIFESRTSLCEKLRMHLQN---- 180
             I++LI+EGK+F+    +G+  P LP T SST P DYQ+ ESRT + EK++  L N    
Sbjct: 121  DILQLIEEGKKFE---KVGHPAP-LPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVN 180

Query: 181  -------------LMSTRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQ 240
                          +   ++ + LE  LFD VI V VG+S  V++IQ +I  +LN  L  
Sbjct: 181  KVGVCGMAGVGKTKLLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSM 240

Query: 241  GEKVRSSRGQ--THVIVEKKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIG 300
              + +  R     + +VE K  IL +LDDLW +YD+E E  IPC SE   EG CKIL+  
Sbjct: 241  KAETKEGRAPFLRNKLVEMKDKILIMLDDLWNEYDLEKEVGIPCRSESSKEG-CKILMTS 300

Query: 301  RLRDLFTNHMDIKELIEVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPI 360
            R RD+ TN M+ K+  +VN L+E+ESW FF+ IVG+   +   I+ + K+VVK+CGGLPI
Sbjct: 301  RSRDILTNKMNTKKCFQVNSLSEDESWKFFMAIVGE--FDKSRIEHIAKNVVKKCGGLPI 360

Query: 361  ALKIIATILKGKRVEIWKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIED-EAQLLLL 420
            ALKIIA  LKGK + IWKDA  KL+  V+V I GVSDQLYSCL+ SY+ IED E +LL L
Sbjct: 361  ALKIIAKALKGKEIHIWKDAFEKLEKHVLVKIKGVSDQLYSCLKLSYDWIEDEETRLLFL 420

Query: 421  LCSVFPDDALINVKDLQMYAMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLL--QGDHDY 480
            LCSVFPDD  I VKDLQMYAMGMGLLN +N W   +NRVI LVDDLKSSYLL      D 
Sbjct: 421  LCSVFPDDHNICVKDLQMYAMGMGLLNDINNWEATKNRVIDLVDDLKSSYLLLESNSGDN 480

Query: 481  FVQMHDVVHDFAKYTASKKDKMTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKL 540
            +VQMHDVV D AKY AS  DKM+SL+Y  GQSEW EEDR GSYNAI+ DC  F +LP+  
Sbjct: 481  YVQMHDVVRDVAKYIASNDDKMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNF 540

Query: 541  EFPNLQLFIL---INIGSLETDFQISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKN 600
            EFPNLQL IL      G  E   QI   FFEGMEKL+VL +++M F PS  TS+THSLKN
Sbjct: 541  EFPNLQLLILGMPSFFGEGEA-IQIPGAFFEGMEKLRVLDMTKMCFEPSR-TSVTHSLKN 600

Query: 601  LQTFCLSNCYFVDIDTIGKLKKLKILKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVI 660
            LQT C+S C + DIDTIG+LKKLKILKI+ C  EELP  M +LTQL++L +S C NLKVI
Sbjct: 601  LQTLCMSYCEYYDIDTIGQLKKLKILKINDCTFEELPANMSQLTQLRLLDLSNCSNLKVI 660

Query: 661  HPDIISKLTRLEELCFHENEFEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVE 720
              +IIS +T+LEEL    + F++WGEE+SY+NKLI N KLSELN L  LS L L I NV 
Sbjct: 661  PINIISSMTKLEELNLWRS-FKRWGEEISYRNKLIQNVKLSELNHLSRLSNLRLEIPNVN 720

Query: 721  ILSNELSLETTRNLKEFNI---STDWIWERGSSNA-ILKLAIKSPIDSIGGVLQILLQKC 780
            I S++LSLE    L+EF I   +  W +      A  L + IKS I +IGG LQILL+ C
Sbjct: 721  IFSDDLSLERVEKLEEFCICVGNDSWGFRICDGYARRLMVNIKSQIVAIGGTLQILLEGC 780

Query: 781  KYLTLDDSVGFTKLFLNPHENYIYPRVENLKLYIRRRIGNLS-PHNFNLQVAFPRLETLH 840
            + L ++D+VGFT      + NY  PR+++L +      GN   P   ++QV+FP L+ L 
Sbjct: 781  EQLYIEDTVGFTNCLFKLNGNYCNPRLKHLHIE-----GNSEMPRRIDVQVSFPELKELT 840

Query: 841  IFGGNHFETLWHNNVQTPNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQCEL 900
            I GGN+ E LWHNN    NS CKL+ + I  CNKLTY+F S MV S V L TL I  CEL
Sbjct: 841  IQGGNNLEMLWHNNRLIANSFCKLRSICISCCNKLTYMFTSNMVTSLVFLNTLEIDNCEL 900

Query: 901  LERIFEIDQVSIDDV---------------------NQAVQSKYSTNLNEVVVLGCPELK 960
            LERIFEI ++ +DD                      N   +     NL  V V  CP LK
Sbjct: 901  LERIFEIGKL-VDDAKVLPLTDLSLRSLPNLKYVWNNDPGEFLTFPNLKNVNVSSCPRLK 960

Query: 961  TLFPASFINHMKEIEILEFDGKNGIFGETDEASQLVSSETVIFPSLRKLSIHCKVVKERR 1020
             LFPASFI H++EIE L     + IF E DEAS+L   E ++F SL+ L    ++V +R 
Sbjct: 961  CLFPASFIKHIEEIESLCAYEVDEIFSE-DEASKLGFPE-IVFRSLQIL----EMVVKRS 1020

Query: 1021 FWVSLKSFPRLEVLRLTGSKDDGMISFPLEMSELLTSVEDLYISNAFELVQVFQNGEYNN 1080
            FW    SFP+L  L+L G +DD +++ PLEMS+ L ++E                     
Sbjct: 1021 FWFRSGSFPKLHNLKLKGGEDDDLVTLPLEMSQELYNIE--------------------- 1080

Query: 1081 NNNSSGTCAKLKSLRLFNLASLTQVWEENSDDQMTSSSCSDSLETIYVERCENLKCLVPS 1140
                                                           V++C+ LK L+PS
Sbjct: 1081 -----------------------------------------------VKKCDKLKFLLPS 1140

Query: 1141 SITFFNLTSVRVEDCNGMMNLFDSYSVAKNLVNLERIKIKGCREMRHIV---VAPEGGEE 1189
            S TFFNL S+ +EDCNGMMNLF++  VAKNLVNL  IKI  CR +  IV   V  E GEE
Sbjct: 1141 STTFFNLGSLIIEDCNGMMNLFNTL-VAKNLVNLCLIKICDCRGITSIVAEEVEEEEGEE 1157

BLAST of PI0003812 vs. ExPASy TrEMBL
Match: A0A0A0K5R3 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G239020 PE=4 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.1e-194
Identity = 514/1382 (37.19%), Postives = 709/1382 (51.30%), Query Frame = 0

Query: 14   EYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERDEGNAIFDDVTKWL 73
            +Y++KPI ++L  +  YN N  + +E+L+ LETT+  + + V   + +   IF  V++WL
Sbjct: 18   KYVIKPIGNQLGYIVFYNRNKNEIKEQLESLETTKKDLDLRVEDAKSKAYTIFTKVSEWL 77

Query: 74   AKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELIDEGKRFDHGSNIGYHL 133
                +E+  ++E    N  C N ++RHQ+SRKA+KR   I  L D G  F     +G   
Sbjct: 78   VAADDEIKKSDELFNSNPPCLNFLQRHQLSRKARKRATDIRRLKDGGNNF---LEVGCPA 137

Query: 134  PFLPQTTSSTRPEDYQIFESRTSLCEKLRMHL-----------------QNLMSTRLEYV 193
            P LP T ++  PE YQ   S+TS+ ++++  L                 +  +   ++ +
Sbjct: 138  P-LPDTMNTIVPEAYQTLGSKTSMAKQIKDALAKPEVRKVGIYGMGGVGKTYLLKEVKKL 197

Query: 194  KLEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVIVEKKKNIL 253
             LE+ LFD VI V VGQS  V+ +Q++I   LNK+L + ++ R+S    + +VE K NIL
Sbjct: 198  VLEEKLFDLVIDVTVGQSNDVMNMQQQIGDFLNKELPKSKEGRTS-FLRNALVEMKGNIL 257

Query: 254  FILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEE 313
               DDLW ++D+ N+  IP   E      CK LV  R +++  N M+IKE  +V CL +E
Sbjct: 258  ITFDDLWNEFDIINDVGIPLSKE-----GCKTLVTSRFQNVLANKMNIKECFKVTCLDDE 317

Query: 314  ESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKR--VEIWKDALN 373
            ESW FF  I+GD+   D  ++ + K V K+CGGLP+AL IIA  LK  R     W+  L+
Sbjct: 318  ESWKFFKKIIGDEF--DAKMENIAKEVAKQCGGLPLALDIIAKTLKRSRHINYYWEGVLS 377

Query: 374  KLKNPVVVDIPGVSDQLYSCLQFSYNSIE-DEAQLLLLLCSVFPDDALINVKDLQMYAMG 433
            KLKN + V+I  V +++Y+ L+ SY  ++ +E + L LLCSVFPDD  I+V DLQMY MG
Sbjct: 378  KLKNSIPVNI-DVGEKVYASLKLSYEHLDGEEVKSLFLLCSVFPDDHGISVNDLQMYVMG 437

Query: 434  MGLLNHVNTWNDARNRVIKLVDDLKSSYLLQGDHDYFVQMHDVVHDFAKYTASKKDKMTS 493
            MGLL  VNTW +AR     LV+DL SS LLQ   +  V+MHD+V D A Y     +  T 
Sbjct: 438  MGLLKMVNTWKEARAEAHYLVEDLTSSSLLQRLKNRDVKMHDIVRDVAIYIGPDFNMSTL 497

Query: 494  LTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFIL-INIGSLETDFQIS 553
                S  S+  +ED+  SY AI+ DC  F NL   L+ P L+L IL       + +  I 
Sbjct: 498  YYGYSTSSKGLDEDKCRSYRAIFVDCKKFCNLLPNLKLPKLELLILSFPFWGKDRNIDIM 557

Query: 554  DTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKIL 613
            D +FEGME L+VL +   SF    WT     LKNL+T C+S C+  DIDTIG LK+L+IL
Sbjct: 558  DAYFEGMENLKVLDIEGTSFLQPFWT----PLKNLRTLCMSYCWCEDIDTIGHLKQLEIL 617

Query: 614  KISSCE-IEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHENEFEQWG 673
            +IS+C  I ELP  M EL QLKVL VS C  L VIH +IIS +T+LEEL   ++ F++WG
Sbjct: 618  RISNCRGITELPTSMSELKQLKVLVVSHCFKLVVIHTNIISSMTKLEELDI-QDCFKEWG 677

Query: 674  EEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNELSLETTRNLKEFNIST---- 733
            EEV YKN  I NA+LSELNCL HLS L +R+  + ILS  LS +  +NL+EF I      
Sbjct: 678  EEVRYKNTWIPNAQLSELNCLSHLSILRVRVLKLTILSEALSSQMLKNLREFFIYVGTHE 737

Query: 734  -------DW-IWERGSSNAILKLAIKSPIDSIGGV-LQILLQKCKYL-TLDDSVGFTK-L 793
                    W  +++   N  +   +KS I S+ G  L ILL+  K L  L+DS GF   +
Sbjct: 738  PKFHPFKSWSSFDKYEKN--MSFNMKSQIVSVNGTKLSILLEGTKRLMILNDSKGFANDI 797

Query: 794  F------------LNPHENYIYPRVE-----NLKLYIRRRIGNL-------SPHN-FN-- 853
            F            L  H+N   P +      +LK  +  R+  L       SP N FN  
Sbjct: 798  FKAIGNGYPLLKCLEIHDNSETPHLRGNDFTSLKRLVLDRMVMLESIIPRHSPINPFNKL 857

Query: 854  ------------------------------------------------------------ 913
                                                                        
Sbjct: 858  KFIKIGRCEQLRNFFPLSVFKGLSNLRQIEIYECNMMEEIVSIEIEDHITIYTSPLTSLR 917

Query: 914  ---------------------------LQVAFPRLETLHIFGGNHFETLWHNNVQTPNSL 973
                                        +V+FP L+ L I   N+ E LWH N    +S 
Sbjct: 918  IERVNKLTSFCSTKSSIQQTIVPLFDERRVSFPELKYLSIGRANNLEMLWHKN---GSSF 977

Query: 974  CKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQCELLERIFEIDQVSIDDVNQAVQS 1033
             KL+ +EI  C +L  VFPS +  S V L TL+I  CELLE IFEI++       + V  
Sbjct: 978  SKLQTIEISDCKELRCVFPSNIATSLVFLDTLKIYGCELLEMIFEIEKQKTSGDTKVVPL 1037

Query: 1034 KYST----------------------NLNEVVVLGCPELKTLFPASFINHMKEIEILEF- 1093
            +Y +                      NL +V V  CP+LK +FPASF  +MKEIE LE  
Sbjct: 1038 RYLSLGFLKNLKYVWDKDVDDVVAFPNLKKVKVGRCPKLKIIFPASFTKYMKEIEELEMV 1097

Query: 1094 DGKNGIFGETDEASQLVSSETVIFPSLRKLSIHCKVVKERRFWVSLKSFPRLEVLRLTGS 1153
            +  N      DEAS+L   E  +F SL  L + CK   + RFWV  K F +L+ L L G 
Sbjct: 1098 EPFNYEIFPVDEASKL--KEVALFQSLETLRMSCKQAVKERFWVMSKFF-KLKSLELFGC 1157

Query: 1154 KDDGMISFPLEMSELLTSVEDLYISNAFELVQVFQNGEYNNNNNSSGTCAKLKSLRLFNL 1213
            +D  MIS P+EM+E+L S+E+L I    +LV V  N  Y         CA LK L+L+NL
Sbjct: 1158 EDGKMISLPMEMNEVLYSIEELTIRGCLQLVDVIGNDYYIQR------CANLKKLKLYNL 1217

Query: 1214 ASLTQVWEENSDDQMTSSSCSDSLETIYVERCENLKCLVPSSITFFNLTSVRVEDCNGMM 1217
              L  V +  + +QMT++                         TF  L  ++V  CNGM+
Sbjct: 1218 PKLMYVLK--NMNQMTAT-------------------------TFSKLVYLQVGGCNGMI 1277

BLAST of PI0003812 vs. ExPASy TrEMBL
Match: A0A1S3CHN4 (disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC103501064 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 1.4e-193
Identity = 486/1288 (37.73%), Postives = 708/1288 (54.97%), Query Frame = 0

Query: 8    VMGGVVEYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERDEGNAIFD 67
            ++  V+   +KPI  +L  L  YN N ++  ++L+ LETT+  V   V + + +   I +
Sbjct: 3    ILVSVIAATIKPIGHQLGYLVCYNRNKKELRDQLENLETTKRDVNQRVQEAKGKSYTISE 62

Query: 68   DVTKWLAKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELIDEGKRFDHGS 127
            +V+KWLA V   + + +E    N SCFN  RR+Q+SRK +K++  I++L+++   F    
Sbjct: 63   EVSKWLADVDNAI-LHDELSNSNPSCFNLARRYQLSRKREKQVNYILQLMNKRNSF---V 122

Query: 128  NIGYHLPFLPQTTSSTRPEDYQIFESRTSLCEKLRMHLQN-------------------L 187
             +GY  P LP T +   P DYQ+ ES+TSL + ++  L                     L
Sbjct: 123  EVGYRAP-LPDTENIVVPGDYQVLESKTSLAKDIKNALAKPEVKKVGVYGMAGVGKTYLL 182

Query: 188  MSTRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVI 247
               +   +K E  LFD  I V VG+   V +IQ++I  +LN +L + ++ R+S  + + +
Sbjct: 183  NEVKKLVLKGEDRLFDRAIDVRVGRFNDVTEIQEQIGDQLNIELPKSKEGRASFLRNN-L 242

Query: 248  VEKKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELI 307
            V+ + NIL +LDDLWK+YD+  E  IP   E      CK+L+  R +D+ TN+M+ +E  
Sbjct: 243  VKMEGNILILLDDLWKEYDLLKEIGIPLSKE-----GCKVLMTSRSQDILTNNMNTQECF 302

Query: 308  EVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKRVEI 367
            +V+ L+EEESW FF+ I+GD+  +    + + K+V KECGGLP+AL  IA  LKGK +  
Sbjct: 303  QVSSLSEEESWKFFMAIIGDKF-DTIYKKNIAKNVAKECGGLPLALDTIAKALKGKDMHH 362

Query: 368  WKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIE-DEAQLLLLLCSVFPDDALINVKDL 427
            W+DAL KL++ + +DI GVSD++Y+ L+ SY  ++ +E +LL LLCSVF DD  I +KDL
Sbjct: 363  WEDALTKLRDSIGMDIKGVSDKVYASLRLSYEHLDGEETKLLFLLCSVFADDYKIPIKDL 422

Query: 428  QMYAMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLL----QGDHDYFVQMHDVVHDFAKY 487
            QMYAMGM LLN V TW D++NRV+KLV+DL SS LL        D +V+MH+VV D A +
Sbjct: 423  QMYAMGMRLLNKVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHNVVRDVAIH 482

Query: 488  TASKKDKMTSLTYKSGQ-SEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFIL-IN 547
             ASK+  M++      + +EWE+E R GS+ AI+ +C  F NLP K+ FP L+L IL ++
Sbjct: 483  IASKEGNMSTFNIGYNKVNEWEDEYRSGSHRAIFANCDNFNNLPLKMNFPQLELLILRVS 542

Query: 548  IGSLETDFQISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDID 607
               +E + QI   FF+GMEKL+VL L+ M      WT  T SL NL+T C+  C F DID
Sbjct: 543  NWLVENNLQIPYAFFDGMEKLKVLDLTGMCCLRPLWT--TPSLNNLRTLCMLRCEFNDID 602

Query: 608  TIGKLKKLKILKISSCE-IEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEEL 667
            TIG+LKKL++L+I  C  ++ LP  M +LTQLKVL V  C  L+V+  ++ S +T+LEEL
Sbjct: 603  TIGELKKLEVLRIVKCNMLDHLPPTMSQLTQLKVLEVLNCSKLEVVPANVFSSMTKLEEL 662

Query: 668  CFHENEFEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNELSLETTRNL 727
               ++ F +WGEEV YK++L+ N  +SELNCL  LS L+L   NV+ILS E++ +T + L
Sbjct: 663  KL-QDSFCRWGEEVWYKDRLVKNVTVSELNCLPCLSNLSLESWNVKILS-EINSQTCKKL 722

Query: 728  KEF----NISTDWIWERGSSN--AILKLAIKSPIDSIGGVLQILLQKCKYLTLDDSVG-F 787
            KEF    N S D++  + S+     L L I+S I SI   L+ILLQ+ + L + DS G F
Sbjct: 723  KEFWICSNESDDFLQPKASNEYATTLMLNIESQIGSIDEGLEILLQRSERLIVSDSKGNF 782

Query: 788  TKLFLNPHENYIYPRVENLKLYIRRRIGNLS-PHNFNLQVAFPRLETLHIFGGNHFETLW 847
                  P+    YP ++   L+I    GN   PH       F  L+ L IFG    E + 
Sbjct: 783  INAIFKPNGLNGYPYLK--YLWIIDENGNSEMPHLIGSD--FTSLKYLIIFGMKRLENIV 842

Query: 848  HNNVQTPNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQCELLERI--FEIDQ 907
              ++   +    +K + IQ C ++  +F   + +  + L  + +  C  +E I   EI+ 
Sbjct: 843  PRHISI-SPFKSVKTIAIQFCGQIRNLFSFSIFKDLLDLQEIEVINCGNMEGIVFMEIE- 902

Query: 908  VSIDDVNQAVQSKYSTNLNEVVVLGCPELKTLFPASFINHMKEIEILEFDGKNGIFGETD 967
               D +N       S  L  V      +L +      +    +  I  FDG+        
Sbjct: 903  ---DQLNICTSPLTSLQLENV-----DKLTSFCTKGLVQESSQSIIPFFDGQ-------- 962

Query: 968  EASQLVSSETVIFPSLRKLSI----HCKVVKERRFWVSLKSFPRLEVLRLTGSKDDGMIS 1027
                      V FP L  LSI    + + +  +    +  SF +L+ +R+        + 
Sbjct: 963  ----------VSFPELNDLSIVGGYNLETLWHKNNNPTTNSFCKLQSIRIEQCTQLRCM- 1022

Query: 1028 FPLEMSELLTSVEDLYISNAFELVQVFQNGEYNNNNNSSGTCA-KLKSLRLFNLASLTQV 1087
            FP  M   LTS+  + I +   L ++F   E  N + S  T    L  L L +L +L  V
Sbjct: 1023 FPSNMFASLTSLHTIQIISCASLKRIF---EIENQSFSVKTVVWSLNELHLLDLPNLKHV 1082

Query: 1088 WEENS------------------------DDQMTSSSCSDSLETIYVERCENLKCLVPSS 1147
            W ++                          D    +   DSLE I VE+C+NLK L+PSS
Sbjct: 1083 WRKDIIKILTFPSLKRVKIHGCTKLTHVWKDNTKETRSFDSLERIEVEKCKNLKYLLPSS 1142

Query: 1148 ITFFNLTSVRVEDCNGMMNLFDSYSVAKNLVNLERIKIKGCREMRHIVVA--PEGGEEII 1207
            ITF NL  + ++ CNGMMNLF S +V K LVNL  IK+  C+ MR +V     E  E I+
Sbjct: 1143 ITFLNLKELHIKKCNGMMNLFSS-TVTKKLVNLRSIKVSYCKRMRCMVEGDQEENDEIIV 1202

Query: 1208 FNRLIDLMFDDLPRLVSFHSGKCMLKFPCLKKLCIKGCLEMKTFNSYGMISTPKLSHMET 1226
            F RL  L  D LPRL SFHSGKCML+FPCL+ L IK C EMKTF SYG+I  P+L  M  
Sbjct: 1203 FKRLSRLELDYLPRLDSFHSGKCMLEFPCLESLVIKRCPEMKTF-SYGVIIAPRLHSMWM 1236

BLAST of PI0003812 vs. ExPASy TrEMBL
Match: A0A6J1FVN0 (probable disease resistance protein At4g27220 OS=Cucurbita moschata OX=3662 GN=LOC111448886 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 5.5e-187
Identity = 454/1232 (36.85%), Postives = 679/1232 (55.11%), Query Frame = 0

Query: 14   EYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERDEGNAIFDDVTKWL 73
            EY++KPI  +LS L  YN N  K +E+L+ L+T    VQ  V + R    AI ++V+KWL
Sbjct: 3    EYVIKPIGRQLSYLLCYNRNKNKLKEQLEALKTARRDVQGRVQEARSNAYAIREEVSKWL 62

Query: 74   AKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELIDEGKRFDHGSNIGYHL 133
            A     +     + + N S FN V R+Q+ RK KK++E + ELI +   F     +GY  
Sbjct: 63   ADADNAIHDERSSNSSNPSAFNLVDRYQLGRKTKKKIEALHELIAKRINF---VEVGYPA 122

Query: 134  PFLPQTTSSTRPEDYQIFESRTSLCEKLRMHLQNLMSTR--------------LEYVK-- 193
            P L +T +   P  YQ+ ES+ S+ ++++  LQ    +R              L  VK  
Sbjct: 123  PLL-ETKNIIVPGGYQVLESKISMAKQIKKALQKAEVSRVGVYGMGGVGKTYLLNEVKKM 182

Query: 194  LEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVIVEKKKNILF 253
            + + +FD VI V VGQS  VIQIQ++I   LN KL + ++ R+S  + + + + ++++L 
Sbjct: 183  VLEEVFDRVIEVFVGQSNGVIQIQEQIGDVLNVKLPKSKEGRASFLRNN-LAKMEEDVLV 242

Query: 254  ILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEE 313
            ILDDLWK+YD+  E  IPC S      +CK+L+  R +D+  N+M+  E  +VN L+EEE
Sbjct: 243  ILDDLWKEYDLLKEIGIPCSS------SCKVLMTSRSQDILMNNMNATECFQVNSLSEEE 302

Query: 314  SWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKRVEIWKDALNKLK 373
            SW FF TI+GD+  +  D++ + K V +ECGGLP+AL  IA  LKGK +  WKDAL+KL+
Sbjct: 303  SWKFFTTIIGDEF-DTPDMENLAKMVARECGGLPLALDTIAKALKGKHMNHWKDALSKLR 362

Query: 374  NPVVVDIPGVSDQLYSCLQFSYNS-IEDEAQLLLLLCSVFPDDALINVKDLQMYAMGMGL 433
            NP+ +DI GVSD++Y+ L+ SY+  + +E +L+ LLCSVFPD   I++ DLQMYAMGM L
Sbjct: 363  NPIGMDIKGVSDKVYASLRLSYDHLVGEEPKLVFLLCSVFPDHYKISLNDLQMYAMGMRL 422

Query: 434  LNHVNTWNDARNRVIKLVDDLKS-SYLLQGDHDYFVQMHDVVHDFAKYTASKKDKMTSLT 493
            L ++NTW D +NRV+KLV DLKS S LL+   D +V+MHD+V D A + AS +  M +L+
Sbjct: 423  LRNINTWEDVKNRVLKLVHDLKSCSLLLEHSSDKYVKMHDIVRDVAIHIASTEGIMPTLS 482

Query: 494  YKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLETDFQISDTF 553
            + S  S+WE+E + G++ AI+ +C  F  LP  L FP+L+L IL +      + +I D F
Sbjct: 483  FGSKLSDWEDEYQSGAHRAIFVNCDNFQKLPQNLNFPDLELLILRSSNGFVGNLEIPDGF 542

Query: 554  FEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKILKIS 613
            F GMEK++ L ++ MSF  +SW     SLKN++T CL  C F  IDTIG+LK L+IL+I 
Sbjct: 543  FAGMEKIKALDITGMSFLQTSW----RSLKNIRTLCLLRCEFNHIDTIGELKNLEILRIC 602

Query: 614  SC-EIEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHENEFEQWGEEV 673
            SC E++ LPV M +LT+LKVL V  C NL+V   +IIS + +LE L   ++ F++WGEEV
Sbjct: 603  SCKELDHLPVAMNQLTELKVLEVLNCSNLEVFPANIISSMNKLEVLKLRDS-FDRWGEEV 662

Query: 674  SYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNELSLETTRNLKEF----NISTDWI 733
             YK+++I N  LSELN L  LS L L I     +S +++ ET+  LKEF    N + D+I
Sbjct: 663  WYKDEVIKNVTLSELNRLPRLSSLRLEISKFRFMS-QITSETSSKLKEFWVCVNKTDDFI 722

Query: 734  WERGSSNAILKLAIKSPI-DSIGGVLQILLQKCKYLTLDDSVG-FTKLFLNPHENYIYPR 793
                S      L +   +  +I G L+ LL++ + L + DSVG F     NP+ N  YP 
Sbjct: 723  LPMVSDEYATTLILNIVLPKAIHGGLETLLKRSERLVVRDSVGRFINDVFNPNSNG-YPF 782

Query: 794  VENLKLYIRRRIGNLSPHNFNLQVAFPRLETLHIFGGNHFETLWHNNVQTPNSLCKLKHL 853
            ++ L +       NL P+ F  +  F  LE L ++G +    L   N        KLK +
Sbjct: 783  LKYLWIIDVHGHSNL-PYLFRSE--FNSLEFLTVYGMDRLMNLAPRN-SPILPFKKLKSI 842

Query: 854  EIQGCNKLTYVFPSCMVRSRVSLITLRIRQCELLERIFEIDQVSIDDVNQAVQSKYSTNL 913
             I+ C ++  +F   ++++   L  + +  C +++ I  I+           QS   T +
Sbjct: 843  SIRSCGEIRNLFSISVLKALSDLQDISVTDCGMMDGIVSIETEE--------QSTIFTGI 902

Query: 914  NEVVVLGCPELKTLFPASFINHMKEIEILEFDGKNGIFGETDEASQLVSSETVIFPSLRK 973
              + +    +LK+     FI    +  I  FD                  + V FP L+ 
Sbjct: 903  TSLTLEKVDKLKSFCTKLFIQEDPQSRIPCFD------------------QRVSFPELKH 962

Query: 974  LSIHCKVVKERRFWVSLKSFPRLEVLRLTGSKDDGMISFPLEMSELLTSVEDLYISNAFE 1033
            LSI                   LE L                                  
Sbjct: 963  LSI--------------TRGNNLETL---------------------------------- 1022

Query: 1034 LVQVFQNGEYNNNNNSSGTCAKLKSLRLFNLASLTQVWEENSDDQMTSSSCSDSLETIYV 1093
                     ++++  +S + +KL+++R+ +   L  ++  N+  ++      DSLE I V
Sbjct: 1023 ---------WHSDGLASNSFSKLQTVRIESCKRLRFMFPSNTSARL------DSLERIEV 1082

Query: 1094 ERCENLKCLVPSSITFFNLTSVRVEDCNGMMNLFDSYSVAKNLVNLERIKIKGCREMRHI 1153
            ++C NLK L+PSS+ F++L+ + VE CNGMMNLF S +VAK LVNL  I+++ CR MR +
Sbjct: 1083 KKCGNLKRLLPSSVLFWSLSELSVEKCNGMMNLF-SLTVAKGLVNLWSIEVRCCRGMRCV 1120

Query: 1154 VVAPEGGEEIIFNRLIDLMFDDLPRLVSFHSGKCMLKFPCLKKLCIKGCLEMKTFNSYGM 1213
            V A E    ++F  L  +    LP LVSF+ GKC ++FP L +L I GC +MKTF S G+
Sbjct: 1143 VAAEEEQGGVVFRGLRRVRLYWLPELVSFYGGKCRVRFPKLDRLFILGCPKMKTF-SRGV 1120

Query: 1214 ISTPKLSHMETEEDIISVSPSHGLNVIIEEIW 1221
            + TPKL  +  +     V+   G+N  I + W
Sbjct: 1203 VVTPKLDIVRIDVRYFEVAVDGGVNETIRDFW 1120

BLAST of PI0003812 vs. ExPASy TrEMBL
Match: A0A0A0LTN4 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 7.2e-187
Identity = 471/1256 (37.50%), Postives = 694/1256 (55.25%), Query Frame = 0

Query: 8    VMGGVVEYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERDEGNAIFD 67
            ++  V+   +KPI  +L  L  YN N ++  E+L+ LETT+  V   V + + +   I +
Sbjct: 3    ILVSVIAATIKPIGHQLGYLVCYNRNKKELREQLENLETTKKDVNQRVEEAKGKSYTISE 62

Query: 68   DVTKWLAKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELIDEGKRFDHGS 127
            +V+KWLA V   +   +E    N SCFN  +R+Q+SRK +K++  I++L+++   F    
Sbjct: 63   EVSKWLADVDNAI-THDELSNSNPSCFNLAQRYQLSRKREKQVNYILQLMNKRNSF---V 122

Query: 128  NIGYHLPFLPQTTSSTRPEDYQIFESRTSLCEKLRMHLQN-------------------L 187
             +GY  P LP T ++  P DYQ+ ES+T L + ++  L                     L
Sbjct: 123  EVGYRAP-LPDTENTVVPGDYQVLESKTLLAKDIKNALSKPEVNKIGVYGMAGVGKTYFL 182

Query: 188  MSTRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVI 247
               +   +K E  LFD VI V VG+   V  IQ++I  +LN +L + ++ R+S  + + +
Sbjct: 183  NEVKKLVLKGEDRLFDRVIDVRVGRFNDVTDIQEQIGDQLNVELPKSKEGRASFLRNN-L 242

Query: 248  VEKKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELI 307
             + + NIL +LDDLWK+YD+  E  IP   +      CK+L+  R +D+ TN+M+ +E  
Sbjct: 243  AKMEGNILILLDDLWKEYDLLKEIGIPLSKD-----GCKVLITSRSQDILTNNMNTQECF 302

Query: 308  EVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKRVEI 367
            +V+ L+EEESW FF+ I+GD+  +    + + K+V KECGGLP+AL  IA  LKGK +  
Sbjct: 303  QVSSLSEEESWKFFMAIIGDKF-DTIYKKNIAKNVAKECGGLPLALDTIAKALKGKDMHH 362

Query: 368  WKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIE-DEAQLLLLLCSVFPDDALINVKDL 427
            W+DAL KL+N + +DI GVSD++Y+ L+ SY+ ++ +E +L+ LLCSVFPDD  I++K+L
Sbjct: 363  WEDALTKLRNSIGMDIKGVSDKVYASLRLSYDHLDGEETKLIFLLCSVFPDDYKISIKNL 422

Query: 428  QMYAMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLL----QGDHDYFVQMHDVVHDFAKY 487
            QMYAM M LLN V TW D++NRV+KLV+DL SS LL        D +V+MHDVV D A +
Sbjct: 423  QMYAMCMRLLNKVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHDVVRDVAIH 482

Query: 488  TASKKDKMTSLTYKSGQ-SEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFIL-IN 547
             ASK+  M++L     + +EWE+E R GS+ AI+ +C    NLP K+ FP L+L IL ++
Sbjct: 483  IASKEGNMSTLNIGYNKVNEWEDECRSGSHRAIFANCDNLNNLPLKMNFPQLELLILRVS 542

Query: 548  IGSLETDFQISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDID 607
               +E + QI   FF+GM KL+VL L+ M      WT  T SL NLQ  C+  C F DID
Sbjct: 543  YWLVEDNLQIPYAFFDGMVKLKVLDLTGMCCLRPLWT--TPSLNNLQALCMLRCEFNDID 602

Query: 608  TIGKLKKLKILKISSCE-IEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEEL 667
            TIG+LKKL++L+I  C  ++ LP  M +LT LKVL V  C  L+V+  +I S +T+LEEL
Sbjct: 603  TIGELKKLEVLRIVKCNMLDHLPPTMSQLTHLKVLEVLNCPKLEVVPANIFSSMTKLEEL 662

Query: 668  CFHENEFEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNELSLETTRNL 727
               ++ F +WGEEV YK++L+ N  +SELNCL  LS L+L   NV+ILS E+S +T + L
Sbjct: 663  KL-QDSFCRWGEEVWYKDRLVKNVTVSELNCLPCLSNLSLESWNVKILS-EISSQTCKKL 722

Query: 728  KEF----NISTDWIWERGSSN--AILKLAIKSPIDSIGGVLQILLQKCKYLTLDDSVG-F 787
            KEF    N S D+I  + S+     L L I+S + SI   L+ILLQ+ + L + DS G F
Sbjct: 723  KEFWICSNESDDFIQPKVSNEYATTLMLNIESQVGSIDEGLEILLQRSERLIVSDSKGNF 782

Query: 788  TKLFLNPHEN------YIYPRVENLKLYIRRRIGNLSPHNFNLQVAFPRLETLHIFGGNH 847
                  P+ N      Y++   EN    +   IG+           F  L+ L IFG   
Sbjct: 783  INAMFKPNGNGYPCLKYLWMIDENGNSEMAHLIGS----------DFTSLKYLIIFGMKR 842

Query: 848  FETLWHNNVQTPNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQCELLERI-- 907
             E +   ++   +   K+K + IQ C ++  +F   + +  + L  + +  C  +E I  
Sbjct: 843  LENIVPRHISL-SPFKKVKTIAIQFCGQIRNLFSFSIFKDLLDLQEIEVINCGKMEGIIF 902

Query: 908  FEI-DQVSIDDVNQAVQSKYSTNLNEVVVLGCPELKTLFPASFINHMKEIEILEFDGKNG 967
             EI DQ++I           S  L  + +    +L +      I    +  I  FDG+  
Sbjct: 903  MEIGDQLNI----------CSCPLTSLQLENVDKLTSFCTKDLIQESSQSIIPFFDGQ-- 962

Query: 968  IFGETDEASQLVSSETVIFPSLRKLSIHCKVVKERRFWVSLKSFPRLEVLRLTGSKDDGM 1027
                            V FP L  LSI                        + G+  + +
Sbjct: 963  ----------------VSFPELNDLSI------------------------VGGNNLETL 1022

Query: 1028 ISFPLEMSELLTSVEDLYISNAFELVQVFQNGEYNNNNNSSGTCAKLKSLRLFNLASLTQ 1087
                   + +L S+ +L++ N   L QV     +  +     T   LK +++     LT 
Sbjct: 1023 WHKNNNPTTVLWSLNELHLLNLPNLKQV-----WRKDIIKILTFPSLKRVKIHGCTKLTH 1082

Query: 1088 VWEENSDDQMTSSSCSDSLETIYVERCENLKCLVPSSITFFNLTSVRVEDCNGMMNLFDS 1147
            VW++N  +++T S   DSLE I VE+C+NLK L+PSSI F NL  + ++ CNGM+NLF S
Sbjct: 1083 VWKDN--NKVTRS--FDSLERIEVEKCKNLKYLLPSSIAFLNLKELHIKKCNGMINLFSS 1142

Query: 1148 YSVAKNLVNLERIKIKGCREMRHIVVA--PEGGEEIIFNRLIDLMFDDLPRLVSFHSGKC 1207
             +V K LVNL  IK+  C+ MR +V     E  E I F +L  L  D LPRL SF+SGKC
Sbjct: 1143 -TVTKKLVNLSSIKVSYCKGMRCMVEVDQAENDEIITFKKLSTLELDYLPRLDSFYSGKC 1168

Query: 1208 MLKFPCLKKLCIKGCLEMKTFNSYGMISTPKLSHMETEEDIISV-SPSHGLNVIIE 1218
            ML+FPCL+ L IK C EMKTF SYG+I  P+L  +   +    V SP+ G+N  I+
Sbjct: 1203 MLEFPCLESLVIKRCPEMKTF-SYGVIIAPRLQTLWMNDKEFGVSSPACGINETIQ 1168

BLAST of PI0003812 vs. NCBI nr
Match: XP_038887230.1 (probable disease resistance protein At5g63020 [Benincasa hispida])

HSP 1 Score: 1325.5 bits (3429), Expect = 0.0e+00
Identity = 768/1303 (58.94%), Postives = 926/1303 (71.07%), Query Frame = 0

Query: 8    VMGGVVEYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERDEGNAIFD 67
            V+G + +Y +KPIL +L  LFHY  NI++ E+KL IL TTE  VQI V+Q++ +GNAIFD
Sbjct: 76   VVGVLADYTIKPILHQLDYLFHYKGNIKEVEKKLGILRTTERTVQILVNQDKGKGNAIFD 135

Query: 68   DVTKWLAKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELIDEGKRFDHGS 127
             VT WL  VK  +DMA +N+ PN SCFNCV+RHQ+SRKAKKRLE I ELI+EG++F+   
Sbjct: 136  GVTNWLDNVKNVLDMAQQNEAPNPSCFNCVQRHQLSRKAKKRLEDINELINEGRQFNK-D 195

Query: 128  NIGYHLPFLPQTTSSTRPEDYQIFESRTSLCEKLRMHLQNL--------------MSTRL 187
            N+GY +P  P T+SST P DYQ FESRTS+ EK++  L N                +T L
Sbjct: 196  NVGYPVP-SPDTSSSTLPADYQFFESRTSMAEKIKDALANPNVNKVGVCGMGGVGKTTLL 255

Query: 188  EYVK---LEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKL--EQGEKVRSSRGQTHVI 247
            + VK   LE  LFD VI VEVGQSKSVI IQ+EIR +LN +    + E+VR+SR QTH I
Sbjct: 256  KEVKKLVLENKLFDRVIQVEVGQSKSVIHIQEEIRAKLNMEYLNMEFEEVRTSRLQTH-I 315

Query: 248  VEKKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELI 307
             EKK+NILF+LDDLWK+YDVE EF IP  S+ RIEG CKIL+  R RDLFTN M+I++L 
Sbjct: 316  AEKKENILFMLDDLWKEYDVEKEFGIPYRSDSRIEG-CKILMTSRRRDLFTNQMNIEKLF 375

Query: 308  EVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKRVEI 367
            EVN LT+EESW FF  I+GD+ VED  ++Q+ K+V+ ECGGLPIALKI+   LKG+RVEI
Sbjct: 376  EVNSLTKEESWKFFSNIIGDEFVEDGYMKQIAKYVINECGGLPIALKILGKTLKGRRVEI 435

Query: 368  WKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIEDEAQLLLLLCSVFPDDALINVKDLQ 427
            WKDAL KLKNPVVVDI GVSDQLYSCLQFSY+SIE+EA+LLLLLCSVFPDD  I V DLQ
Sbjct: 436  WKDALKKLKNPVVVDIHGVSDQLYSCLQFSYDSIENEAKLLLLLCSVFPDDHRIYVDDLQ 495

Query: 428  MYAMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLLQGD---HDYFVQMHDVVHDFAKYTA 487
             YAMGMGLLN  NTW DA+NRVIKLVDDLKSSYLL       D +V+MHDVVHDFAKY A
Sbjct: 496  KYAMGMGLLNQTNTWEDAKNRVIKLVDDLKSSYLLLAPPEWDDNYVKMHDVVHDFAKYIA 555

Query: 488  SKKDKMTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIG-S 547
            SK DKMTS TY +GQ  WEE++RHGSYNAIY D TTFLNLP KLEFPNLQLFILI+ G  
Sbjct: 556  SKIDKMTSFTYGNGQRAWEEDNRHGSYNAIYVDPTTFLNLPPKLEFPNLQLFILISNGLG 615

Query: 548  LETDFQISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIG 607
                 Q  +T FEGM++L+VL +    F P    SL +SLKNL+  CLS+C   DID IG
Sbjct: 616  PRPTIQNLETVFEGMKELRVLDVRMWYFEP----SLIYSLKNLRVLCLSHCDSNDIDAIG 675

Query: 608  KLKKLKILKISSCE-IEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFH 667
            +LK+LKIL+I  C  I+E P C  +LTQLKVL VS C NLKVIHP+IISK+TRLE+L F 
Sbjct: 676  ELKRLKILEIHFCRGIQEFPECTSKLTQLKVLQVSHCMNLKVIHPNIISKMTRLEDLSF- 735

Query: 668  ENEFEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTL-RIQNVEILSNELSLETTRNLKE 727
             + F+ WGEEV YK KLI NAKLSELNCL HL  L L  ++NVEILSNELSLE+T+ L+ 
Sbjct: 736  SHSFDGWGEEVWYKQKLIRNAKLSELNCLTHLVHLELCNLKNVEILSNELSLESTQKLET 795

Query: 728  FNIST-DWIW-----ERGSS--------NAILKLAIKSPIDSIGGVLQILLQKCKYLTLD 787
              I   D  W     +R +            L L I SPIDSIGGV QILLQKC+ L L 
Sbjct: 796  CRIFVGDERWNIHYNDRNNPYNRKDKRYTTALNLYIDSPIDSIGGVFQILLQKCEKLVLR 855

Query: 788  DSVGFTKLFLNPHENYIYPRVENLKL-YIRRRIGNLSPHNFNLQVAFPRLETLHIFGGNH 847
            DSVGFT L  N + N  YP +++L++ Y    IGN+SP NF+LQVAFP+L++L+I G N+
Sbjct: 856  DSVGFTMLLCNTYANSCYPLLKHLEIDYPPAIIGNISPLNFDLQVAFPQLKSLYIGGRNN 915

Query: 848  FETLWHNNVQTPNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQCELLERIFE 907
            FETLW NN  T N   KL+ + I+GCNK TYVFPS MVRS V L  L +R CEL+ERIFE
Sbjct: 916  FETLWRNNGLTENYFSKLERIFIEGCNKFTYVFPSRMVRSLVFLNRLVVRYCELVERIFE 975

Query: 908  IDQVSIDDVNQ-----------------------AVQSKYSTNLNEVVVLGCPELKTLFP 967
            I++ +   VNQ                       A++     NL +V V  CP+LKTLFP
Sbjct: 976  IEESTSIIVNQDVPLTILELSSLPNLKYVWNNDPALELLTFPNLKKVKVEECPQLKTLFP 1035

Query: 968  ASFINHMKEIEILEFDGKNGIFGETDEASQLVSSETVIFPSLRKLSIHCKVVKERRFWVS 1027
             SFINHMKE E L+  G+  IFGETDEASQLVS +  IFPSLR L++H  ++K R FW  
Sbjct: 1036 VSFINHMKEFEELDCVGETEIFGETDEASQLVSPQ--IFPSLRYLTVHYTLMKRRSFWFR 1095

Query: 1028 LKSFPRLEVLRLTGSKDDGMISFPLEMSELL---TSVEDLYISNAFELVQVFQNGEYNNN 1087
             +SF  L  LRL GS+DD M+S P EMSE     + V  L I NAFE V++F+NGE NN 
Sbjct: 1096 PESFSELLTLRLIGSEDDDMVSLPFEMSESAVWRSVVRHLQIENAFEWVKIFRNGEENNQ 1155

Query: 1088 N--NSSGTCAKLKSLRLFNLASLTQVWEENSDDQMTSSSC-SDSLETIYVERCENLKCLV 1147
            N   SS   AKLK L L+NL  LT +WE+ S DQMT++SC  D LE+I V+RC+NLKCL+
Sbjct: 1156 NYHASSSGGAKLKHLCLYNLPKLTHIWED-SIDQMTATSCFVDILESIVVDRCDNLKCLL 1215

Query: 1148 PSSITFFNLTSVRVEDCNGMMNLFDSYSVAKNLVNLERIKIKGCREMRHIVVAPEG---G 1207
            PSSITF NLT + VE CNGMMNLF S SVAKNLVNL  + I  C EMR IVV P+G   G
Sbjct: 1216 PSSITFSNLTFLDVERCNGMMNLFGS-SVAKNLVNLREMWINECTEMRSIVVQPKGKEEG 1275

Query: 1208 EEIIFNRLIDLMFDDLPRLVSFHSGKCMLKFPCLKKLCIKGCLEMKTFNSYGMISTPKLS 1239
            EEIIF++L +L   +L RL SFHS KC+LKFP L+KL I GC EMKTF SYG+ISTPKLS
Sbjct: 1276 EEIIFDKLNELKLSNLTRLASFHSEKCVLKFPSLEKLKISGCPEMKTF-SYGIISTPKLS 1335

BLAST of PI0003812 vs. NCBI nr
Match: XP_022143564.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 953.7 bits (2464), Expect = 1.6e-273
Identity = 595/1251 (47.56%), Postives = 764/1251 (61.07%), Query Frame = 0

Query: 1    MDTVIGGVMGGVVEYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERD 60
            MD  I  V   VV+Y V PI  +L+ LF YN NI+  E++L+ L+ T +RVQ+ V++ R 
Sbjct: 1    MDVFI-SVGAKVVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARS 60

Query: 61   EGNAIFDDVTKWLAKVKEEMDMAN---------ENKTPNISCFNCVRRHQISRKAKKRLE 120
                I+D+V+ WL  V+ ++++ N            + N+SC N V+RH++SRK+KKR++
Sbjct: 61   NAYEIYDEVSTWLL-VEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVK 120

Query: 121  KIIELIDEGKRFDHGSNIGYHLPFLPQTTSSTRPEDYQIFESRTSLCEKLRMHLQN---- 180
             I++LI+EGK+F+    +G+  P LP T SST P DYQ+ ESRT + EK++  L N    
Sbjct: 121  DILQLIEEGKKFE---KVGHPAP-LPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVN 180

Query: 181  -------------LMSTRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQ 240
                          +   ++ + LE  LFD VI V VG+S  V++IQ +I  +LN  L  
Sbjct: 181  KVGVCGMAGVGKTKLLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSM 240

Query: 241  GEKVRSSRGQ--THVIVEKKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIG 300
              + +  R     + +VE K  IL +LDDLW +YD+E E  IPC SE   EG CKIL+  
Sbjct: 241  KAETKEGRAPFLRNKLVEMKDKILIMLDDLWNEYDLEKEVGIPCRSESSKEG-CKILMTS 300

Query: 301  RLRDLFTNHMDIKELIEVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPI 360
            R RD+ TN M+ K+  +VN L+E+ESW FF+ IVG+   +   I+ + K+VVK+CGGLPI
Sbjct: 301  RSRDILTNKMNTKKCFQVNSLSEDESWKFFMAIVGE--FDKSRIEHIAKNVVKKCGGLPI 360

Query: 361  ALKIIATILKGKRVEIWKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIED-EAQLLLL 420
            ALKIIA  LKGK + IWKDA  KL+  V+V I GVSDQLYSCL+ SY+ IED E +LL L
Sbjct: 361  ALKIIAKALKGKEIHIWKDAFEKLEKHVLVKIKGVSDQLYSCLKLSYDWIEDEETRLLFL 420

Query: 421  LCSVFPDDALINVKDLQMYAMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLL--QGDHDY 480
            LCSVFPDD  I VKDLQMYAMGMGLLN +N W   +NRVI LVDDLKSSYLL      D 
Sbjct: 421  LCSVFPDDHNICVKDLQMYAMGMGLLNDINNWEATKNRVIDLVDDLKSSYLLLESNSGDN 480

Query: 481  FVQMHDVVHDFAKYTASKKDKMTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKL 540
            +VQMHDVV D AKY AS  DKM+SL+Y  GQSEW EEDR GSYNAI+ DC  F +LP+  
Sbjct: 481  YVQMHDVVRDVAKYIASNDDKMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNF 540

Query: 541  EFPNLQLFIL---INIGSLETDFQISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKN 600
            EFPNLQL IL      G  E   QI   FFEGMEKL+VL +++M F PS  TS+THSLKN
Sbjct: 541  EFPNLQLLILGMPSFFGEGEA-IQIPGAFFEGMEKLRVLDMTKMCFEPSR-TSVTHSLKN 600

Query: 601  LQTFCLSNCYFVDIDTIGKLKKLKILKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVI 660
            LQT C+S C + DIDTIG+LKKLKILKI+ C  EELP  M +LTQL++L +S C NLKVI
Sbjct: 601  LQTLCMSYCEYYDIDTIGQLKKLKILKINDCTFEELPANMSQLTQLRLLDLSNCSNLKVI 660

Query: 661  HPDIISKLTRLEELCFHENEFEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVE 720
              +IIS +T+LEEL    + F++WGEE+SY+NKLI N KLSELN L  LS L L I NV 
Sbjct: 661  PINIISSMTKLEELNLWRS-FKRWGEEISYRNKLIQNVKLSELNHLSRLSNLRLEIPNVN 720

Query: 721  ILSNELSLETTRNLKEFNI---STDWIWERGSSNA-ILKLAIKSPIDSIGGVLQILLQKC 780
            I S++LSLE    L+EF I   +  W +      A  L + IKS I +IGG LQILL+ C
Sbjct: 721  IFSDDLSLERVEKLEEFCICVGNDSWGFRICDGYARRLMVNIKSQIVAIGGTLQILLEGC 780

Query: 781  KYLTLDDSVGFTKLFLNPHENYIYPRVENLKLYIRRRIGNLS-PHNFNLQVAFPRLETLH 840
            + L ++D+VGFT      + NY  PR+++L +      GN   P   ++QV+FP L+ L 
Sbjct: 781  EQLYIEDTVGFTNCLFKLNGNYCNPRLKHLHIE-----GNSEMPRRIDVQVSFPELKELT 840

Query: 841  IFGGNHFETLWHNNVQTPNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQCEL 900
            I GGN+ E LWHNN    NS CKL+ + I  CNKLTY+F S MV S V L TL I  CEL
Sbjct: 841  IQGGNNLEMLWHNNRLIANSFCKLRSICISCCNKLTYMFTSNMVTSLVFLNTLEIDNCEL 900

Query: 901  LERIFEIDQVSIDDV---------------------NQAVQSKYSTNLNEVVVLGCPELK 960
            LERIFEI ++ +DD                      N   +     NL  V V  CP LK
Sbjct: 901  LERIFEIGKL-VDDAKVLPLTDLSLRSLPNLKYVWNNDPGEFLTFPNLKNVNVSSCPRLK 960

Query: 961  TLFPASFINHMKEIEILEFDGKNGIFGETDEASQLVSSETVIFPSLRKLSIHCKVVKERR 1020
             LFPASFI H++EIE L     + IF E DEAS+L   E ++F SL+ L    ++V +R 
Sbjct: 961  CLFPASFIKHIEEIESLCAYEVDEIFSE-DEASKLGFPE-IVFRSLQIL----EMVVKRS 1020

Query: 1021 FWVSLKSFPRLEVLRLTGSKDDGMISFPLEMSELLTSVEDLYISNAFELVQVFQNGEYNN 1080
            FW    SFP+L  L+L G +DD +++ PLEMS+ L ++E                     
Sbjct: 1021 FWFRSGSFPKLHNLKLKGGEDDDLVTLPLEMSQELYNIE--------------------- 1080

Query: 1081 NNNSSGTCAKLKSLRLFNLASLTQVWEENSDDQMTSSSCSDSLETIYVERCENLKCLVPS 1140
                                                           V++C+ LK L+PS
Sbjct: 1081 -----------------------------------------------VKKCDKLKFLLPS 1140

Query: 1141 SITFFNLTSVRVEDCNGMMNLFDSYSVAKNLVNLERIKIKGCREMRHIV---VAPEGGEE 1189
            S TFFNL S+ +EDCNGMMNLF++  VAKNLVNL  IKI  CR +  IV   V  E GEE
Sbjct: 1141 STTFFNLGSLIIEDCNGMMNLFNTL-VAKNLVNLCLIKICDCRGITSIVAEEVEEEEGEE 1157

BLAST of PI0003812 vs. NCBI nr
Match: XP_038887227.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 818.5 bits (2113), Expect = 8.0e-233
Identity = 459/793 (57.88%), Postives = 562/793 (70.87%), Query Frame = 0

Query: 8   VMGGVVEYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERDEGNAIFD 67
           V+G VVEY ++PIL +L  LF YN N++K +EK K LETT D VQI VSQ++ +GNAIFD
Sbjct: 4   VVGVVVEYTIRPILHQLGYLFRYNGNVKKLQEKHKTLETTRDTVQILVSQDKGKGNAIFD 63

Query: 68  DVTKWLAKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELIDEGKRFDHGS 127
           +VTKWL  VKE  +M  +NK  N SCFN V+RH++SRK+KK +E I ELI+EG+RF+   
Sbjct: 64  EVTKWLDNVKEVSNMTKQNKELNPSCFNFVKRHRLSRKSKKMVEDIAELINEGRRFNK-D 123

Query: 128 NIGYHLPFLPQTTSSTRPEDYQIFESRTSLCEKLRMHLQN-----------------LMS 187
            IGY +P    T   T P DYQI ESRT L E ++  L N                  + 
Sbjct: 124 RIGYPVP-SSDTNCPTIPTDYQIIESRTLLVENIKDALANPNVNKVGVCGMGGVGKTSLL 183

Query: 188 TRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVIVE 247
             ++ + LE NLFD VI VEVGQSKSVI IQ+EIR  LN    Q  K+ +S  QTH I+E
Sbjct: 184 NEVKKLVLENNLFDRVIQVEVGQSKSVIDIQEEIRGALNM---QCGKITASLLQTH-IIE 243

Query: 248 KKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEV 307
           +K+NILF+LDDLWK YD+E EF IPC SE R EG CKIL+  R R   TN M+I++L EV
Sbjct: 244 RKENILFMLDDLWKDYDLEKEFGIPCRSESRTEG-CKILMTSRSRHTLTNQMNIEKLFEV 303

Query: 308 NCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKRVEIWK 367
           N +T++ESW FF  IVG + VED  IQQ+ K VV ECGGLPIALK+IA  LKGKRVEIWK
Sbjct: 304 NPVTKKESWMFFTAIVG-EYVEDGYIQQIAKDVVNECGGLPIALKVIAKALKGKRVEIWK 363

Query: 368 DALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIEDEAQLLLLLCSVFPDDALINVKDLQMY 427
           DAL KLKNPVVV+I GVSD+LYSCLQFSY+SIEDEA+L+LLLCSVFPDD  I VKDLQMY
Sbjct: 364 DALKKLKNPVVVNISGVSDRLYSCLQFSYDSIEDEAKLVLLLCSVFPDDHRIYVKDLQMY 423

Query: 428 AMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLL----QGDHDYFVQMHDVVHDFAKYTAS 487
           A+GMG LNH+NTW DARNRVIKLVDDLKSS LL    + + + +V++HDV+HDF KY AS
Sbjct: 424 AVGMGFLNHINTWEDARNRVIKLVDDLKSSNLLLAPDEEEGEEYVKIHDVIHDFGKYIAS 483

Query: 488 KKDKMTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLE 547
           K DKM+SLTY+SGQ E +E DRHG+YNAIY +C+ FLN P KLE PNLQL IL N+    
Sbjct: 484 KTDKMSSLTYRSGQKELQENDRHGTYNAIYLNCSEFLNYPQKLELPNLQLLILRNVLLDY 543

Query: 548 TDFQISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKL 607
               I   FFEGM  ++VL +          TSLT SLKNLQT CLSNC  +DIDTI +L
Sbjct: 544 KVIPIPVAFFEGMNGVRVLDVRVAHLGQPQLTSLTQSLKNLQTLCLSNCGSIDIDTISQL 603

Query: 608 KKLKILKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHENE 667
           KKL+IL+I  C I+E+ V M ELTQLKVL  + C +LKVIHP++I+ +TRLEEL   E +
Sbjct: 604 KKLEILRIHHCIIKEVTVTMSELTQLKVLDFANCSSLKVIHPNVIANMTRLEELNL-ETD 663

Query: 668 FEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNELSLETTRNLKEF--N 727
           F++WGE VSYK KLI NA+LSELNCL HL  L + I N+  LSNELS+E  +NL++F  +
Sbjct: 664 FKRWGENVSYKKKLIKNAELSELNCLTHLLYLNVTIPNIHTLSNELSIERVQNLEKFCIH 723

Query: 728 ISTDWIWERGSSNAILKLAIKSPIDSIGGVLQILLQKCKYLTLDDSVGFTKLFLNPHENY 777
           +   +  +      IL L IKSPIDSIG +  ILLQKC+ L + DSVGFTK+  NP  N 
Sbjct: 724 VGAGYAMKNERWARILILHIKSPIDSIGEIPHILLQKCEQLDVQDSVGFTKIIYNPSGNN 783

BLAST of PI0003812 vs. NCBI nr
Match: XP_038890209.1 (probable disease resistance protein At4g27220 [Benincasa hispida])

HSP 1 Score: 804.3 bits (2076), Expect = 1.6e-228
Identity = 547/1356 (40.34%), Postives = 776/1356 (57.23%), Query Frame = 0

Query: 55   VSQERDEGNAIFDDVTKWLAKVKEEMDMAN---ENKTPNIS-------CFNCVRRHQISR 114
            V++ R     I  DV++WL +V + ++ +N   +N+  N S         N +  +Q+S+
Sbjct: 2    VNEARSNAYNIESDVSEWLIQVDKIIERSNMWYKNEKDNGSNGKYFSNKLNFIHHYQVSK 61

Query: 115  KAKKRLEKIIELIDEGKRFDHGSNIGY--HLPFLPQTTSSTRPEDY-QIFESRTSLCEKL 174
            KAKK ++ I +++++ K F     +GY   LP +  + SST    Y QI ESRTSL +++
Sbjct: 62   KAKKMVKVISQILEKRKMF---HQVGYPAPLPRIIGSGSSTTTHGYDQILESRTSLAKQI 121

Query: 175  RMHL-----------------QNLMSTRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEI 234
            R  L                 +  +  ++  + +E+ LFD VI+V VGQ+  V  IQ +I
Sbjct: 122  RDALADHNVNRVGVYGMGGVGKTTLLKQVTPLVMEEKLFDQVIIVNVGQTLGVEGIQAQI 181

Query: 235  RVELNKKLEQGEKVRSSRGQTHVI---VEKKKNILFILDDLWKKYDVENEFVIPCCSELR 294
              +L  +LE  +KV S  G+  +I   +E + NIL +LDDLW+  ++E +  IPC SEL 
Sbjct: 182  GDKL--RLELNKKVESKEGRASLIQNKLEMESNILLVLDDLWRGLNLE-DIGIPCRSELC 241

Query: 295  IEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGK 354
             +G CKIL+  R +D+ TN MD +   EV  L+E+ESW FF+ ++G +  +++ ++ +GK
Sbjct: 242  KKG-CKILITSRDKDVLTNEMDTQVYFEVKPLSEKESWEFFMNMIGGEF-DNKCVELIGK 301

Query: 355  HVVKECGGLPIALKIIATILKGKRVEIWKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNS 414
             +V++CGGLPIAL  I   LKGK   IWKDAL +LKNP+ VD+ GVSD +Y+ L+ SY+ 
Sbjct: 302  EMVRKCGGLPIALATIVKALKGKEEAIWKDALKQLKNPITVDVKGVSDVVYASLKLSYDY 361

Query: 415  IE-DEAQLLLLLCSVFPDDALINVKDLQMYAMGMGLLNHVNTWNDARNRVIKLVDDLKSS 474
            ++ +EA+LL LLCSVFPDD  I+V+DLQ+YAMG+ LLN VNTW++ARNRVIKLVDDLK+S
Sbjct: 362  LKGEEARLLFLLCSVFPDDYEISVEDLQIYAMGLRLLNQVNTWDEARNRVIKLVDDLKAS 421

Query: 475  YLL--QGDHDYFVQMHDVVHDFAKYTASKKDKMTSLTYKSGQSEWEEEDRHGSYNAIYQD 534
             LL      D  V+MHD V D A Y ASK+  M++L+Y  G SEW+E+DRHGSY AI+ +
Sbjct: 422  SLLLESNSRDNHVKMHDTVRDVAIYIASKEANMSTLSYGFGLSEWQEKDRHGSYRAIFGN 481

Query: 535  CTTFLNLPTKLEFPNLQLFILINIGSLETDFQISDTFFEGMEKLQVLRLSQMSFAPSSWT 594
            C  F NLP  LEFP L+L IL          QI D FFEGM++L+VL LS+MSF  + W 
Sbjct: 482  CHNFYNLPQNLEFPKLELLILDGHDWRGEKLQICDPFFEGMKELKVLNLSRMSF-QTLWR 541

Query: 595  SLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKILKISSCEIEELPVCMRELTQLKVLHVS 654
            S  +SL+NLQT C+S C F DID I  LKKL+IL++  C I  LP+ M +LTQLKVL VS
Sbjct: 542  SSIYSLENLQTLCMSYCTFNDIDAISHLKKLQILRVDKCSITLLPISMSQLTQLKVLQVS 601

Query: 655  FCDNLKVIHPDIISKLTRLE--ELCFHENEFEQWGEEVSYKNKLITNAKLSELNCLKHLS 714
             C  LKVI P+IIS + +L+  ++C     F  WGEEV Y NKLI NA+ SEL CL HL+
Sbjct: 602  NCP-LKVIPPNIISSMLKLQALDIC---TSFNGWGEEVLYNNKLINNARPSELKCLSHLT 661

Query: 715  CLTLRIQNVEILSNELSLETTRNLKEFNISTDWI--------WERGSSNAILK-LAIKSP 774
             L L I ++ IL++ L  +  + L+ F I    +         E+ ++  +LK +   S 
Sbjct: 662  NLKLHILDINILADLLFFKNLK-LERFVIHIGDLKMSQRLQGCEQYATTLMLKIITSSSQ 721

Query: 775  IDSIG--GVLQILLQKCKYLTLDDSVGFTKLFLNPHENYIYPRVENLKL----YIRRRIG 834
            I SI    VL+ILL++ + L ++ S+  T +   P+    YP + +L L     +R  IG
Sbjct: 722  IVSIDHHEVLEILLKQSENLCVEGSMMVTNIHFKPNNGNNYPYLRHLTLTKDSKLRHLIG 781

Query: 835  N---------------------------LSPHNF-------------------------- 894
            N                           +S + F                          
Sbjct: 782  NGCFAYFPSLEFLSLEKMESLENIVHVQVSTNPFRKLRSIKVISCKRLRYLFSFSIFKGL 841

Query: 895  -NLQ-----------------------------VAFPRLETLHIFGGNHFETLWHNNVQT 954
             +LQ                             VAFP+L+ L I G N+ +TLWH N   
Sbjct: 842  VDLQKVFIYDCNMMDEIFFMDSEDSTIKAECNCVAFPQLKDLTIIGANNLKTLWHKNGLA 901

Query: 955  PNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQCELLERIFEIDQVS------ 1014
            PN   KL+ + I GCN L Y+FPS +V + VSL TL +  C LL+ IFE++  S      
Sbjct: 902  PNFFTKLQRISINGCNNLRYIFPSNVVVALVSLNTLVVESCYLLDGIFEMENTSNFKQKD 961

Query: 1015 --IDDVNQAV-----------QSKYSTN-----------LNEVVVLGCPELKTLFPASFI 1074
               DD  Q +             KY  N           L +V+ + CP+L+TLFPAS I
Sbjct: 962  DDDDDDAQVIPLVEVNLKHLPNLKYVWNKDVGKFLKFPRLRDVIAINCPQLQTLFPASLI 1021

Query: 1075 NHMKEIEILEFDGKNGIFGETDEASQLVSSETVIFPSLRKLSIHCKVVKERRFWVS---- 1134
             H+ +++ L   G + +F E DE S+ +  E ++FPSL  LS+  K +    FW++    
Sbjct: 1022 KHVHQLQNLVVLGIHKMFSE-DETSKSMFHE-ILFPSLTGLSMDTKAM-TTNFWLTQFSK 1081

Query: 1135 LKSFPRLEVLRLTGSKDDGMISFPLEMSELLTSVEDLYISNAFELVQVFQNGEYNNNNNS 1194
             +SFP L  L L GS DD M + PLEM+++L +++ L +S   +LVQ+F N E    +N 
Sbjct: 1082 SRSFPNLRNLSLMGSYDDEMTTLPLEMTQILYNIKVLNVSFVSQLVQIFSNEE--EISNP 1141

Query: 1195 SGTCAKLKSLRLFNLASLTQVWEENSDDQMTSSSCSDSLETIYVERCENLKCLV-PSSIT 1235
               CA LK L L +L +L  VW++N   QMT     D+LE I+VE C  LKCLV PSSIT
Sbjct: 1142 IQRCADLKKLTLSHLPNLKHVWKDNI--QMT--ILFDNLEAIHVEECGKLKCLVPPSSIT 1201

BLAST of PI0003812 vs. NCBI nr
Match: XP_038900638.1 (LOW QUALITY PROTEIN: probable disease resistance protein At4g27220 [Benincasa hispida])

HSP 1 Score: 781.9 bits (2018), Expect = 8.3e-222
Identity = 538/1396 (38.54%), Postives = 753/1396 (53.94%), Query Frame = 0

Query: 1    MDTVIGGVMGGVVEYIVKPILDELSCLFHYNENIEKFEEKLKILETTEDRVQIFVSQERD 60
            MD  + G++  V + + KPI  +L  +  +N N E+ +E+L+ LETT+  V   V + R 
Sbjct: 5    MDVAV-GIVTEVGKCVFKPIGHQLGYIVFFNRNKEELKEQLENLETTKRDVHRRVEEARS 64

Query: 61   EGNAIFDDVTKWLAKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELIDEG 120
               AI  +V+KWL  V   + + +E    N SCFN  +RHQ+SRK KKR   ++ELI++ 
Sbjct: 65   NSYAIHAEVSKWLVDVGNAL-VHDELSNSNPSCFNLHQRHQLSRKTKKRTTHVLELINKR 124

Query: 121  KRFDHGSNIGYHLPFLPQTTSSTRPEDYQIFESRTSLCEKLRMHL--------------- 180
              F     +G   P LP T ++  PE YQ+  S+TS+ ++++  L               
Sbjct: 125  NNF---VEVGCPEP-LPDTENTIIPEGYQVLGSKTSMAKQIKDALAKPEVNKVGVYGMGG 184

Query: 181  --QNLMSTRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRG 240
              +  +   ++ + LE+NLFD VI V +GQS  V+++Q++I   LN +L + ++ ++S  
Sbjct: 185  VGKTYLLNEVKTLVLEENLFDRVIDVTIGQSNGVMEMQQQIGDILNIELPRSKEGKAS-F 244

Query: 241  QTHVIVEKKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHM- 300
              + ++E K +IL + DDLWK+YD+  E  IP   E      CK+L+  R +++ TN+M 
Sbjct: 245  LRNKLMEMKGSILVMFDDLWKEYDLIKEVGIPLGKE-----GCKVLMTSRDQNILTNNMI 304

Query: 301  DIKELIEVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIATILK 360
            +  E  EV CL EEESW FF TI+GD+  +   ++ + K VV+ECGGLP+AL  IA  LK
Sbjct: 305  NRTECFEVTCLNEEESWKFFTTIIGDKF-DTTYMEIIAKEVVRECGGLPLALDTIAKALK 364

Query: 361  GKRVEIWKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIE-DEAQLLLLLCSVFPDDAL 420
             KR+  W+DAL+KLK    V I GV+ ++Y+ L+ SY+ ++ DE+++L LLCSVFPDD +
Sbjct: 365  EKRINYWEDALSKLKTSNAVGIKGVTHKVYASLRLSYDYLDGDESKILFLLCSVFPDDYM 424

Query: 421  INVKDLQMYAMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLLQGD------HDYFVQMHD 480
            I+ KDLQ+YAMGM LL  VNTW +A+N +IKLVDDL SS LLQ        +D +V+MHD
Sbjct: 425  ISTKDLQVYAMGMRLLRRVNTWGEAKNSIIKLVDDLTSSXLLQVQVYKDTRYDQYVKMHD 484

Query: 481  VVHDFAKYTASKKDKMTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQ 540
            +V D A Y AS    M++L Y S  SEW + D  GSY AI+ DC    N    ++FP L+
Sbjct: 485  IVRDVAIYIASTTTNMSTLYYGSKHSEWPDTDNCGSYRAIFIDCKNICNFLPSMKFPKLE 544

Query: 541  LFIL-INIGSLETDFQISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSN 600
            L IL  +    E   +  D FFEGM+ L+VL L+++      W+    SLK L+T CLS 
Sbjct: 545  LLILRFSTKLSEVCIETIDAFFEGMKNLKVLDLTKIHVPSVLWS----SLKKLRTLCLSQ 604

Query: 601  CYFVDIDTIGKLKKLKILKISSCE-IEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISK 660
            C   DID I  LK+L+IL+I SCE I ELP  M EL QLKVL VS C NL VIHP+IIS 
Sbjct: 605  CECKDIDKISHLKELEILRIISCEGITELPATMSELKQLKVLVVSKCYNLVVIHPNIISS 664

Query: 661  LTRLEELCFHENEFEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNELS 720
            LT+LEEL   +  F++WGE+V YKN  I NA+L E+N L HLS L L+I    +   +L 
Sbjct: 665  LTKLEELDI-QLCFQKWGEKVRYKNTWIQNARLLEVNYLPHLSILKLQISKANVGLEDLG 724

Query: 721  LETTRNLKEFNI--STDWI----WERGS----SNAILKLAIKSPIDSIGGVLQILLQKCK 780
             +T + L+EF I   TD      W+  S       +L + ++  +   G V   L +  +
Sbjct: 725  SQTLKKLREFFICVGTDHFKFESWKNNSDKYEKTFLLNMELQMSLVD-GMVFDKLFEGTE 784

Query: 781  YLTL-DDSVGFTKLFLNPHEN--------------------------------------- 840
             L + +DS GF  + L  + N                                       
Sbjct: 785  QLKIVNDSKGFANVILKANGNGYPLLKCLMIDGNSKMSHLIGNDFTSLECLSLSEMMLLE 844

Query: 841  --------------------------------YIYPRVENL------------------- 900
                                             I+  + NL                   
Sbjct: 845  SIVPKHASTNPFNKLIVIQIGSCKQLRNLFSFSIFKGLSNLQEIKVTYCDMMDEIVSVVE 904

Query: 901  ----------------------------KLYIRRRIGNLSPHNFNLQVAFPRLETLHIFG 960
                                        K +I++R   + P  F+ QV+FP ++ L I G
Sbjct: 905  IEDQTTICTFPLTTLHLEGLNKVTSFCTKPFIQQRPQTIIPF-FDQQVSFPEMKNLTIKG 964

Query: 961  GNHFETLWHNNVQTPNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQCELLER 1020
            GN+ E LWHN+  T +S CKL+ + I  CNKL Y+FP  +V S   L TL I  CELLE 
Sbjct: 965  GNNLEMLWHNDGPTASSFCKLRSISIGNCNKLRYIFPWNIVTSLALLDTLFIFSCELLES 1024

Query: 1021 IFEIDQVSID----------DVNQAVQSKYSTN-----------LNEVVVLGCPELKTLF 1080
            IFEI++ S+           D+N     KY  N           L EV+V  CPE+K++F
Sbjct: 1025 IFEIEKPSVGDTIAMSLRKVDLNGLPNLKYMWNRDVCDVMAFPKLKEVLVTDCPEMKSIF 1084

Query: 1081 PASFINHMKEIEILEFDGKNGIFGETDEASQLVSSETVIFPSLRKLSIHCKVVKERRFWV 1140
            PASF  +++ +E LE D +N IF   D+A +L   E V+F +LR L + CK+  +  FWV
Sbjct: 1085 PASFPQYIEAVERLEVDEQNEIF-PVDKALKL--GEVVLFRNLRSLKMTCKLATKESFWV 1144

Query: 1141 SLKSFPRLEVLRLTGSKD-DGMISFPLEMSELLTSVEDLYISNAFELVQVFQNGEYNNNN 1200
             +  F +LE + L G ++ D M+S PLEMSE+L ++E+L I   F+LV +F  G    N+
Sbjct: 1145 -ISKFLKLESITLVGYEEGDKMVSLPLEMSEVLYNIEELKIETCFQLVYIF--GNETEND 1204

Query: 1201 NSSGTCAKLKSLRLFNLASLTQVWEENSDDQMTSSSCSDSLETIYVERCENLKCLVPSSI 1219
            N    CA LK L+L NL  LT VW+  + +QMT                         +I
Sbjct: 1205 NIQRRCAHLKKLKLKNLPKLTNVWK--NVNQMT-------------------------TI 1264

BLAST of PI0003812 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 186.4 bits (472), Expect = 1.4e-46
Identity = 219/909 (24.09%), Postives = 407/909 (44.77%), Query Frame = 0

Query: 19  PILDELSCLFHYN-----ENIEKFEEKLKIL-ETTEDRVQIFVSQERDEGNAIFDD---- 78
           P++ E+  L + +      N  KF+  +K L E+ E   ++  +   D    +  D    
Sbjct: 6   PVIGEILRLMYESTFSRVANAIKFKSNVKALNESLERLTELKGNMSEDHETLLTKDKPLR 65

Query: 79  --VTKWLAKVKEEMDMANENKTPNISCFNCVRRHQISRKAKKRLEKI-------IELID- 138
             + +W  + +E +  A       +SC     R ++SRK  K L+++       IE +D 
Sbjct: 66  LKLMRWQREAEEVISKARLKLEERVSC-GMSLRPRMSRKLVKILDEVKMLEKDGIEFVDM 125

Query: 139 -------------EGKRFDHGSNIGYHLPFLPQTTSSTRPEDYQIF----ESRTSLCEKL 198
                         G    H +     L  +    +S + +   ++      +T+L   L
Sbjct: 126 LSVESTPERVEHVPGVSVVHQTMASNMLAKIRDGLTSEKAQKIGVWGMGGVGKTTLVRTL 185

Query: 199 RMHLQNLMSTRLEYVKLEKNLFDWVIVVEVGQSKSVIQIQKEI--RVELNKKLEQGEKVR 258
              L+   +T+          F  VI V V +     ++QK+I  R++++ ++E+ E+  
Sbjct: 186 NNKLREEGATQ---------PFGLVIFVIVSKEFDPREVQKQIAERLDIDTQMEESEEKL 245

Query: 259 SSRGQTHVIVEKKKNILFILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFT 318
           + R   +V + K++  L ILDD+WK  D++    IP   E +     K+++  R  ++  
Sbjct: 246 ARR--IYVGLMKERKFLLILDDVWKPIDLD-LLGIPRTEENK---GSKVILTSRFLEV-C 305

Query: 319 NHMDIKELIEVNCLTEEESWSFFVTIVGDQLVEDEDIQQVGKHVVKECGGLPIALKIIAT 378
             M     + V+CL EE++W  F    GD +V  + ++++ K V +ECGGLP+A+  + T
Sbjct: 306 RSMKTDLDVRVDCLLEEDAWELFCKNAGD-VVRSDHVRKIAKAVSQECGGLPLAIITVGT 365

Query: 379 ILKGKR-VEIWKDALNKLKNPVVVDIPGVSDQLYSCLQFSYNSIEDEAQLLLLLCSVFPD 438
            ++GK+ V++W   L+KL   V   I  + ++++  L+ SY+ +ED+A+   LLC++FP+
Sbjct: 366 AMRGKKNVKLWNHVLSKLSKSVPW-IKSIEEKIFQPLKLSYDFLEDKAKFCFLLCALFPE 425

Query: 439 DALINVKDLQMYAMGMGLLNHVNTWNDARNRVIKLVDDLKSSYLLQ-GDHDYFVQMHDVV 498
           D  I V ++  Y M  G +  + +  D+ N  I  V+ LK   LL+ GD    V+MHDVV
Sbjct: 426 DYSIEVTEVVRYWMAEGFMEELGSQEDSMNEGITTVESLKDYCLLEDGDRRDTVKMHDVV 485

Query: 499 HDFAKY--TASKKDKMTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQ 558
            DFA +  ++S+ D  + +   +G  +  ++    S   +        +LP  +E    +
Sbjct: 486 RDFAIWIMSSSQDDSHSLVMSGTGLQDIRQDKLAPSLRRVSLMNNKLESLPDLVE----E 545

Query: 559 LFILINIGSLETDFQISDT---FFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCL 618
             +  ++  L+ +F + +    F +    L++L LS          SL   L +L +  L
Sbjct: 546 FCVKTSVLLLQGNFLLKEVPIGFLQAFPTLRILNLSGTRIKSFPSCSLLR-LFSLHSLFL 605

Query: 619 SNCY-FVDIDTIGKLKKLKILKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVIHPDII 678
            +C+  V + ++  L KL++L +    I E P  + EL + + L +S   +L+ I   ++
Sbjct: 606 RDCFKLVKLPSLETLAKLELLDLCGTHILEFPRGLEELKRFRHLDLSRTLHLESIPARVV 665

Query: 679 SKLTRLEELCFHENEFEQWGEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNE 738
           S+L+ LE L    + +    +  + K +    A + E+ CL+ L  L++R+ +   L N+
Sbjct: 666 SRLSSLETLDMTSSHYRWSVQGETQKGQ----ATVEEIGCLQRLQVLSIRLHSSPFLLNK 725

Query: 739 LSLETTRNLKEFNI---STDWIWERGSSNAILKLAIKSPIDSIGGVL----QILLQKCKY 798
            +    R LK+F +   S   +  R     +    +     SIG +L     + L  C+ 
Sbjct: 726 RNTWIKR-LKKFQLVVGSRYILRTRHDKRRLTISHLNVSQVSIGWLLAYTTSLALNHCQG 785

Query: 799 L------TLDDSVGFTKLFLNPHENYIYPRVENLKLYIRRRIGNLSPHNFNLQVAFPRLE 858
           +       + D+ GF  L     EN I     N   ++     N S  + ++    P LE
Sbjct: 786 IEAMMKKLVSDNKGFKNLKSLTIENVII----NTNSWVEMVSTNTSKQSSDILDLLPNLE 845

Query: 859 TLHIFGGNHFETLWHNNVQTPNSLCKLKHLEIQGCNKLTYVFPSCMVRSRVSLITLRIRQ 868
            LH+   +  ET           L  LK +EI  C KL  +       +  +L  + I  
Sbjct: 846 ELHLRRVD-LETFSELQTHLGLKLETLKIIEITMCRKLRTLLDKRNFLTIPNLEEIEISY 880

BLAST of PI0003812 vs. TAIR 10
Match: AT4G10780.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 131.7 bits (330), Expect = 4.2e-30
Identity = 144/529 (27.22%), Postives = 236/529 (44.61%), Query Frame = 0

Query: 180 KNLFDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVI-VEKKKNILFI 239
           KN  D VI V V     + +IQ++I  +L    ++  K + S+    ++    KK  + +
Sbjct: 201 KNGVDIVIWVVVSSDLQIHKIQEDIGEKLGFIGKEWNKKQESQKAVDILNCLSKKRFVLL 260

Query: 240 LDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEES 299
           LDD+WKK D+  +  IP  S+ R E  CK++   R  D+    M + + +EV CL+  ++
Sbjct: 261 LDDIWKKVDL-TKIGIP--SQTR-ENKCKVVFTTRSLDVCA-RMGVHDPMEVQCLSTNDA 320

Query: 300 WSFFVTIVGD-QLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKR-VEIWKDALNKL 359
           W  F   VG   L    DI ++ K V  +C GLP+AL +I   + GKR V+ W  A++ L
Sbjct: 321 WELFQEKVGQISLGSHPDILELAKKVAGKCRGLPLALNVIGETMAGKRAVQEWHHAVDVL 380

Query: 360 KNPVVVDIPGVSDQLYSCLQFSYNSIEDE-AQLLLLLCSVFPDDALINVKDLQMYAMGMG 419
            +    +  G+ D +   L++SY+++ D+  +     C+++P+D  I    L  Y +  G
Sbjct: 381 TS-YAAEFSGMDDHILLILKYSYDNLNDKHVRSCFQYCALYPEDYSIKKYRLIDYWICEG 440

Query: 420 LLNHVNTWNDARNRVIKLVDDLKSSYLL--QGDHDYFVQMHDVVHDFAKYTAS---KKDK 479
            ++       A N+  +++  L  + LL  +G +   V+MHDVV + A +T S   K  +
Sbjct: 441 FIDGNIGKERAVNQGYEILGTLVRACLLSEEGKNKLEVKMHDVVREMALWTLSDLGKNKE 500

Query: 480 MTSLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLETDFQ 539
              +   SG  +  + +  G+   +         +    E P L    L    SL     
Sbjct: 501 RCIVQAGSGLRKVPKVEDWGAVRRLSLMNNGIEEISGSPECPELTTLFLQENKSL---VH 560

Query: 540 ISDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLK 599
           IS  FF  M KL VL LS+            H L  L             + I +L  L+
Sbjct: 561 ISGEFFRHMRKLVVLDLSE-----------NHQLDGLP------------EQISELVALR 620

Query: 600 ILKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHENEFEQW 659
            L +S   IE LP C+++L  L  L++     L  I    ISKL+ L  L    +     
Sbjct: 621 YLDLSHTNIEGLPACLQDLKTLIHLNLECMRRLGSIAG--ISKLSSLRTLGLRNS----- 680

Query: 660 GEEVSYKNKLITNAKLSELNCLKHLSCLTLRIQNVEILSNELSLETTRN 700
                  N ++    + EL+ L+HL  LT+ I +  +L   +   T  N
Sbjct: 681 -------NIMLDVMSVKELHLLEHLEILTIDIVSTMVLEQMIDAGTLMN 683

BLAST of PI0003812 vs. TAIR 10
Match: AT1G12220.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 122.9 bits (307), Expect = 2.0e-27
Identity = 168/694 (24.21%), Postives = 302/694 (43.52%), Query Frame = 0

Query: 183 FDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVIVE-----KKKNILF 242
           FD VI V V +S +V +IQ++I     +K+  G    S +    + V+     +++  + 
Sbjct: 207 FDVVIWVVVSRSSTVRKIQRDIA----EKVGLGGMEWSEKNDNQIAVDIHNVLRRRKFVL 266

Query: 243 ILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEE 302
           +LDD+W+K +++    +P  S+   +  CK+    R RD+    M + + +EV+CL  EE
Sbjct: 267 LLDDIWEKVNLK-AVGVPYPSK---DNGCKVAFTTRSRDV-CGRMGVDDPMEVSCLQPEE 326

Query: 303 SWSFFVTIVG-DQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKR-VEIWKDALNK 362
           SW  F   VG + L    DI  + + V ++C GLP+AL +I   +  KR V  W  A++ 
Sbjct: 327 SWDLFQMKVGKNTLGSHPDIPGLARKVARKCRGLPLALNVIGEAMACKRTVHEWCHAIDV 386

Query: 363 LKNPVVVDIPGVSDQLYSCLQFSYNSIEDE-AQLLLLLCSVFPDDALINVKDLQMYAMGM 422
           L +   +D  G+ D++   L++SY+++  E  +   L CS+FP+D LI+ + L  Y +  
Sbjct: 387 LTSS-AIDFSGMEDEILHVLKYSYDNLNGELMKSCFLYCSLFPEDYLIDKEGLVDYWISE 446

Query: 423 GLLNHVNTWNDARNRVIKLVDDLKSSYLL--QGDHDYFVQMHDVVHDFAKYTASKKDKMT 482
           G +N         N+  +++  L  + LL  +  +   V+MHDVV + A + +S   K  
Sbjct: 447 GFINEKEGRERNINQGYEIIGTLVRACLLLEEERNKSNVKMHDVVREMALWISSDLGKQK 506

Query: 483 SLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLETD-FQI 542
                       E  +   +N + +   + +N   +  F + +   L  +   + D  +I
Sbjct: 507 EKCIVRAGVGLREVPKVKDWNTVRK--ISLMNNEIEEIFDSHECAALTTLFLQKNDVVKI 566

Query: 543 SDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKI 602
           S  FF  M  L VL LS+             SL  L             + I +L  L+ 
Sbjct: 567 SAEFFRCMPHLVVLDLSE-----------NQSLNELP------------EEISELASLRY 626

Query: 603 LKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHENEFEQWG 662
             +S   I +LPV +  L +L  L++    +L  I    IS L  L  L   ++      
Sbjct: 627 FNLSYTCIHQLPVGLWTLKKLIHLNLEHMSSLGSILG--ISNLWNLRTLGLRDSRL---- 686

Query: 663 EEVSYKNKLITNAKLSELNCLKHLSCLTLRIQN----VEILSNELSLETTRN-----LKE 722
                   L+  + + EL  L+HL  +TL I +      +L ++  +E  +      LKE
Sbjct: 687 --------LLDMSLVKELQLLEHLEVITLDISSSLVAEPLLCSQRLVECIKEVDFKYLKE 746

Query: 723 FNISTDWIWERGSSNAILKLAIKSPIDSIGGVLQILLQKCKYLTLDDSVGFTKLFLNPHE 782
            ++    +   G+   + KL IK       G+ +I +++    +  +    T  F N   
Sbjct: 747 ESVRVLTLPTMGN---LRKLGIKR-----CGMREIKIERTTSSSSRNKSPTTPCFSNLSR 806

Query: 783 NYI--------------YPRVENLKLYIRRRIGNL------SPHNFNLQVAFPRLETLHI 837
            +I               P +  L++   + + ++        H+  + V F +LETLH+
Sbjct: 807 VFIAKCHGLKDLTWLLFAPNLTFLEVGFSKEVEDIISEEKAEEHSATI-VPFRKLETLHL 838

BLAST of PI0003812 vs. TAIR 10
Match: AT1G12220.2 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 122.9 bits (307), Expect = 2.0e-27
Identity = 168/694 (24.21%), Postives = 302/694 (43.52%), Query Frame = 0

Query: 183 FDWVIVVEVGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVIVE-----KKKNILF 242
           FD VI V V +S +V +IQ++I     +K+  G    S +    + V+     +++  + 
Sbjct: 207 FDVVIWVVVSRSSTVRKIQRDIA----EKVGLGGMEWSEKNDNQIAVDIHNVLRRRKFVL 266

Query: 243 ILDDLWKKYDVENEFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEE 302
           +LDD+W+K +++    +P  S+   +  CK+    R RD+    M + + +EV+CL  EE
Sbjct: 267 LLDDIWEKVNLK-AVGVPYPSK---DNGCKVAFTTRSRDV-CGRMGVDDPMEVSCLQPEE 326

Query: 303 SWSFFVTIVG-DQLVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKR-VEIWKDALNK 362
           SW  F   VG + L    DI  + + V ++C GLP+AL +I   +  KR V  W  A++ 
Sbjct: 327 SWDLFQMKVGKNTLGSHPDIPGLARKVARKCRGLPLALNVIGEAMACKRTVHEWCHAIDV 386

Query: 363 LKNPVVVDIPGVSDQLYSCLQFSYNSIEDE-AQLLLLLCSVFPDDALINVKDLQMYAMGM 422
           L +   +D  G+ D++   L++SY+++  E  +   L CS+FP+D LI+ + L  Y +  
Sbjct: 387 LTSS-AIDFSGMEDEILHVLKYSYDNLNGELMKSCFLYCSLFPEDYLIDKEGLVDYWISE 446

Query: 423 GLLNHVNTWNDARNRVIKLVDDLKSSYLL--QGDHDYFVQMHDVVHDFAKYTASKKDKMT 482
           G +N         N+  +++  L  + LL  +  +   V+MHDVV + A + +S   K  
Sbjct: 447 GFINEKEGRERNINQGYEIIGTLVRACLLLEEERNKSNVKMHDVVREMALWISSDLGKQK 506

Query: 483 SLTYKSGQSEWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLETD-FQI 542
                       E  +   +N + +   + +N   +  F + +   L  +   + D  +I
Sbjct: 507 EKCIVRAGVGLREVPKVKDWNTVRK--ISLMNNEIEEIFDSHECAALTTLFLQKNDVVKI 566

Query: 543 SDTFFEGMEKLQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKI 602
           S  FF  M  L VL LS+             SL  L             + I +L  L+ 
Sbjct: 567 SAEFFRCMPHLVVLDLSE-----------NQSLNELP------------EEISELASLRY 626

Query: 603 LKISSCEIEELPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHENEFEQWG 662
             +S   I +LPV +  L +L  L++    +L  I    IS L  L  L   ++      
Sbjct: 627 FNLSYTCIHQLPVGLWTLKKLIHLNLEHMSSLGSILG--ISNLWNLRTLGLRDSRL---- 686

Query: 663 EEVSYKNKLITNAKLSELNCLKHLSCLTLRIQN----VEILSNELSLETTRN-----LKE 722
                   L+  + + EL  L+HL  +TL I +      +L ++  +E  +      LKE
Sbjct: 687 --------LLDMSLVKELQLLEHLEVITLDISSSLVAEPLLCSQRLVECIKEVDFKYLKE 746

Query: 723 FNISTDWIWERGSSNAILKLAIKSPIDSIGGVLQILLQKCKYLTLDDSVGFTKLFLNPHE 782
            ++    +   G+   + KL IK       G+ +I +++    +  +    T  F N   
Sbjct: 747 ESVRVLTLPTMGN---LRKLGIKR-----CGMREIKIERTTSSSSRNKSPTTPCFSNLSR 806

Query: 783 NYI--------------YPRVENLKLYIRRRIGNL------SPHNFNLQVAFPRLETLHI 837
            +I               P +  L++   + + ++        H+  + V F +LETLH+
Sbjct: 807 VFIAKCHGLKDLTWLLFAPNLTFLEVGFSKEVEDIISEEKAEEHSATI-VPFRKLETLHL 838

BLAST of PI0003812 vs. TAIR 10
Match: AT4G26090.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 118.6 bits (296), Expect = 3.7e-26
Identity = 202/889 (22.72%), Postives = 368/889 (41.39%), Query Frame = 0

Query: 34  IEKFEEKLKILETTEDRVQIFVSQERDEGNAIFDDVTKWLAKVK-------------EEM 93
           I   E  +  L+   D + + + Q+  EG +  +   +WL+ V+                
Sbjct: 35  ITDLETAIGDLKAIRDDLTLRIQQDGLEGRSCSNRAREWLSAVQVTETKTALLLVRFRRR 94

Query: 94  DMANENKTPNISCFNCVRRHQISRKAKKRLEKIIELID--EGKRFDHGS--NIGYHLPFL 153
           +     +   +SCF C   +++ +K    L+ I EL +  E  + D GS       +P  
Sbjct: 95  EQRTRMRRRYLSCFGCA-DYKLCKKVSAILKSIGELRERSEAIKTDGGSIQVTCREIPIK 154

Query: 154 PQTTSSTRPEDYQIFESRTSLCEKLRMH------LQNLMSTRLEYVKLEKNLFDWVIVVE 213
               ++T  E    F S       + ++         LM +    +  + + +D +I V+
Sbjct: 155 SVVGNTTMMEQVLEFLSEEEERGIIGVYGPGGVGKTTLMQSINNELITKGHQYDVLIWVQ 214

Query: 214 VGQSKSVIQIQKEIRVELNKKLEQGEKVRSSRGQTHVIVEKKKNILFILDDLWKKYDVEN 273
           + +      IQ+ +   L    ++ E    +R        ++K  L +LDD+W++ D+E 
Sbjct: 215 MSREFGECTIQQAVGARLGLSWDEKE-TGENRALKIYRALRQKRFLLLLDDVWEEIDLEK 274

Query: 274 EFVIPCCSELRIEGTCKILVIGRLRDLFTNHMDIKELIEVNCLTEEESWSFFVTIV-GDQ 333
             V         E  CK++   R   L  N+M  +  + V  L ++ +W  F + V    
Sbjct: 275 TGV----PRPDRENKCKVMFTTRSIAL-CNNMGAEYKLRVEFLEKKHAWELFCSKVWRKD 334

Query: 334 LVEDEDIQQVGKHVVKECGGLPIALKIIATILKGKRVEIWKDALNKLKNPVVVDIPGVSD 393
           L+E   I+++ + +V +CGGLP+AL  +   +  +  E      +++      ++ G+ +
Sbjct: 335 LLESSSIRRLAEIIVSKCGGLPLALITLGGAMAHRETEEEWIHASEVLTRFPAEMKGM-N 394

Query: 394 QLYSCLQFSYNSIE-DEAQLLLLLCSVFPDDALINVKDLQMYAMGMGLLNHVNTWNDARN 453
            +++ L+FSY+++E D  +   L C++FP++  I ++ L  Y +G G L    T +   N
Sbjct: 395 YVFALLKFSYDNLESDLLRSCFLYCALFPEEHSIEIEQLVEYWVGEGFL----TSSHGVN 454

Query: 454 RVIK---LVDDLKSSYLLQ-GDHDYFVQMHDVVHDFAKYTASKKDKMTSLTY---KSGQS 513
            + K   L+ DLK++ LL+ GD    V+MH+VV  FA + AS++     L       G +
Sbjct: 455 TIYKGYFLIGDLKAACLLETGDEKTQVKMHNVVRSFALWMASEQGTYKELILVEPSMGHT 514

Query: 514 EWEEEDRHGSYNAIYQDCTTFLNLPTKLEFPNLQLFILINIGSLETDFQISDTFFEGMEK 573
           E  + +       I         LP KL  P L   +L    SL+   +I   FF  M  
Sbjct: 515 EAPKAENWRQALVISLLDNRIQTLPEKLICPKLTTLMLQQNSSLK---KIPTGFFMHMPV 574

Query: 574 LQVLRLSQMSFAPSSWTSLTHSLKNLQTFCLSNCYFVDIDTIGKLKKLKILKISSCEIEE 633
           L+VL L        S+TS+T          LS  Y V+         L  L +S  +I  
Sbjct: 575 LRVLDL--------SFTSITE-------IPLSIKYLVE---------LYHLSMSGTKISV 634

Query: 634 LPVCMRELTQLKVLHVSFCDNLKVIHPDIISKLTRLEELCFHEN----EFEQWGEEVSYK 693
           LP  +  L +LK L +     L+ I  D I  L++LE L  + +    E + +GE+ +  
Sbjct: 635 LPQELGNLRKLKHLDLQRTQFLQTIPRDAICWLSKLEVLNLYYSYAGWELQSFGEDEA-- 694

Query: 694 NKLITNAKLSELNCLKHLSCLTLRIQNVEILS------------NELSLETTRNLKEFNI 753
                    ++L  L++L+ L + + ++E L               L +E    L  FN+
Sbjct: 695 ----EELGFADLEYLENLTTLGITVLSLETLKTLFEFGALHKHIQHLHVEECNELLYFNL 754

Query: 754 STDWIWERGSSNAILKLAIKSPID-----SIGGVLQILLQKCKYLTLDDSVGFTKLFLNP 813
            +  +   G +  + +L+IKS  D     +        L   + LTL      T+++ N 
Sbjct: 755 PS--LTNHGRN--LRRLSIKSCHDLEYLVTPADFENDWLPSLEVLTLHSLHNLTRVWGNS 814

Query: 814 HENYIYPRVENLKLYIRRRIGNLSPHNFNLQVAFPRLETLHIFGGNHFETLWHN----NV 866
                   +  + +    ++ N+S          P+LE + +F     E L       +V
Sbjct: 815 VSQDCLRNIRCINISHCNKLKNVS-----WVQKLPKLEVIELFDCREIEELISEHESPSV 867