PI0003610 (gene) Melon (PI 482460) v1

Overview


Name	PI0003610
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	alpha-ketoglutarate-dependent dioxygenase alkB
Location	chr10: 17672446 .. 17674744 (+)
RNA-Seq Expression	PI0003610
Synteny	PI0003610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAAAAAAAAAACTCTAAACGAAAACGAAAAGGCAAACGTGTCTGACCAGTCAAAACCCTAAGCTCAATTATCCTTCTCCCGTCCATCGCCAGAATTCAGAATCTGAATCTTACGCCGATCGAGCATCTCGCCGGCGACAGCGAAACCTGGTGGATAAAATGTACGGATCCGACAAAGGCACCGACGATTCCGAGCGAACCGCTTTCAGAAGAGCAGAAAAGAAATACAAACTGTACTACGACGACACCTACAAATCTTCCAAAAAGTATTCCTTCACAATTGCTCTTTCAGGTTCTATTTCATTCCATCGCCATTGAATTCCTTGCAAGAGACAATGTTTCGAACTCTTTTTTGTGATTCCTCTCAGGAAAAAACTACCGAAACATGTCGATTTGTCTGAGGTTATCGATTTCAAGAACATCCTCGAATCTTACAAACAAGATGGTTCACTTCCAGTGGGCGTGAACGCGACTAAATGCGATCTTGATGGGCCAGTTTTCCGCTTGGAGAATCGTCCTGGTATTGCCGATTCTCCATTTTCGACTCTGTTTCTCAGAAAAAAGATCCCAAATTCAGTTGTTCTTCATGTGTTTAACATGTAGGGTTTTATTTTATTCCTGGAGCGTTGAGTTTACAAGAGCAATGCCAATGGATTAGGGAGAGTTTAATGGATTTCCCGCAGCCTCCCAACAGGACCAACCATAATGCTATTTATGGACCAATTCAAAACCTGTTTATTGCAGCTAAGGGAAAGAAAGTTTTAGTTGAACATGATGAAACTTCTGATTTCAACCTTGATTCTGATGTTGAACCTTCCATTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGAATACTGTTTCATCCAGAAGAGGGACGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTAGAAAGTTGCGTTGGAGTACACTTGGCCTACAATTTGATTGGTCCAAGGTGAGTTTATTTATAAAACTTCATATTGTGCTCGTAGAATACCAATTTAAACTATGTGTTGAATGTTTTTAATGGTTTCAAAACGCTTAAACCTCAGAATATTGTTTCTGTTGCTGGGTTTTGTTATGTTATTTTCTGAATTACTCACCTTGTTATGGCGTAGCGAAGCTATAACATATCTCTGCCCCATAATAAGATACCCCCTGCACTATGTCAACTTGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAATTATTTTGCTTCAGGTAACTAAAGGAAAATTTGTTCATGGGAAGTTAACAAAATCTGCCCTTTGTTGAGGAAGCCACATTTGTTCACGGGTTACTCTACTTATGACCGGTTGTTTCTTTGATGGAACCCATGTTATCATGACTCTTACACGATATTGATATTTCCAGGCGACACGCTCGGTGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGGTAGGCATCCCTCTAATTTGTGCCCTTTCAATTGCATTTCTTGCTGATATGTTAAAAGGGCATGGACTGGTATTGATTCTGTTATCTATTTAATCTGTCCGCCTCCACTTCGTCCAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAATCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTCGTGTTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGTCGCCTTAATGCTCACTCTTACATCATATCAAGATGTATTTCTTTTGTTTGTTATCACCTTGGATATCTGAATCCAAATAATGTCCGAGTGATTCATTTGTAGAAATATCGTTAACTCGCCAACACGTGATGTCGGTGTCTTATAGGTGTACCACGTATCTTCATCGATGAAGAAAGTGAAGAAATTTCTTTTCTCGCAAGGCATTTATCAAATCAAGATGATTCACACTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAAGTTTTCTGATTGATTGGACGTGTTTGTCATCTTTCAATCTTCAAGATGGTTTTCAGGTTGCTCCAAAATTTTGCCTGGATGACGGGGGAAGGGAGTGGCTTCGCAAATTCATAGAGATCGATGACAATCCTATTGCTGACCCAAGATCTATGAAGCTTGGATTCGAGCAGTTGTTTTGGTTGGCAATGGGTGGGGATACTGCAGGACAGGCACAAATAAAGGATTATGATGTGCAAGTTCATCTAGATGATTTAATTTAAAATACGAGAGTTGTTTTACGTCCGC

mRNA sequence

AAGAAAAAAAAAAACTCTAAACGAAAACGAAAAGGCAAACGTGTCTGACCAGTCAAAACCCTAAGCTCAATTATCCTTCTCCCGTCCATCGCCAGAATTCAGAATCTGAATCTTACGCCGATCGAGCATCTCGCCGGCGACAGCGAAACCTGGTGGATAAAATGTACGGATCCGACAAAGGCACCGACGATTCCGAGCGAACCGCTTTCAGAAGAGCAGAAAAGAAATACAAACTGTACTACGACGACACCTACAAATCTTCCAAAAAGAAAAAACTACCGAAACATGTCGATTTGTCTGAGGTTATCGATTTCAAGAACATCCTCGAATCTTACAAACAAGATGGTTCACTTCCAGTGGGCGTGAACGCGACTAAATGCGATCTTGATGGGCCAGTTTTCCGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGAGCGTTGAGTTTACAAGAGCAATGCCAATGGATTAGGGAGAGTTTAATGGATTTCCCGCAGCCTCCCAACAGGACCAACCATAATGCTATTTATGGACCAATTCAAAACCTGTTTATTGCAGCTAAGGGAAAGAAAGTTTTAGTTGAACATGATGAAACTTCTGATTTCAACCTTGATTCTGATGTTGAACCTTCCATTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGAATACTGTTTCATCCAGAAGAGGGACGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTAGAAAGTTGCGTTGGAGTACACTTGGCCTACAATTTGATTGGTCCAAGCGAAGCTATAACATATCTCTGCCCCATAATAAGATACCCCCTGCACTATGTCAACTTGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAATTATTTTGCTTCAGGCGACACGCTCGGTGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAATCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTCGTGTTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTGTACCACGTATCTTCATCGATGAAGAAAGTGAAGAAATTTCTTTTCTCGCAAGGCATTTATCAAATCAAGATGATTCACACTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAAGTTTTCTGATTGATTGGACGTGTTTGTCATCTTTCAATCTTCAAGATGGTTTTCAGGTTGCTCCAAAATTTTGCCTGGATGACGGGGGAAGGGAGTGGCTTCGCAAATTCATAGAGATCGATGACAATCCTATTGCTGACCCAAGATCTATGAAGCTTGGATTCGAGCAGTTGTTTTGGTTGGCAATGGGTGGGGATACTGCAGGACAGGCACAAATAAAGGATTATGATGTGCAAGTTCATCTAGATGATTTAATTTAAAATACGAGAGTTGTTTTACGTCCGC

Coding sequence (CDS)

ATGTACGGATCCGACAAAGGCACCGACGATTCCGAGCGAACCGCTTTCAGAAGAGCAGAAAAGAAATACAAACTGTACTACGACGACACCTACAAATCTTCCAAAAAGAAAAAACTACCGAAACATGTCGATTTGTCTGAGGTTATCGATTTCAAGAACATCCTCGAATCTTACAAACAAGATGGTTCACTTCCAGTGGGCGTGAACGCGACTAAATGCGATCTTGATGGGCCAGTTTTCCGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGAGCGTTGAGTTTACAAGAGCAATGCCAATGGATTAGGGAGAGTTTAATGGATTTCCCGCAGCCTCCCAACAGGACCAACCATAATGCTATTTATGGACCAATTCAAAACCTGTTTATTGCAGCTAAGGGAAAGAAAGTTTTAGTTGAACATGATGAAACTTCTGATTTCAACCTTGATTCTGATGTTGAACCTTCCATTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGAATACTGTTTCATCCAGAAGAGGGACGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTAGAAAGTTGCGTTGGAGTACACTTGGCCTACAATTTGATTGGTCCAAGCGAAGCTATAACATATCTCTGCCCCATAATAAGATACCCCCTGCACTATGTCAACTTGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAACCTGAAGCTGCAATAGTGAATTATTTTGCTTCAGGCGACACGCTCGGTGGTCACCTAGATGACATGGAAGCTGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAATCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTCGTGTTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTGTACCACGTATCTTCATCGATGAAGAAAGTGAAGAAATTTCTTTTCTCGCAAGGCATTTATCAAATCAAGATGATTCACACTTTCTGGAATACATAAGAACTTCAAGAATAAACATCAACATTAGACAAGTTTTCTGA

Protein sequence

MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQDGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNHNAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRGTACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIRQVF

Homology

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BLAST of PI0003610 vs. ExPASy Swiss-Prot
Match: Q9SA98 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 489.6 bits (1259), Expect = 3.1e-137
Identity = 241/363 (66.39%), Postives = 280/363 (77.13%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MY S   +DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I +++  
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DG LP G+  +K D   PVF ++NRPGFYFIP ALSL+EQC+WI+ESL  FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPI +LF +AK  KVLV+ D T+                 + WKF EE  +     
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDLTN-----------------NKWKFYEEVDIEKATR 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           ++CKS+ ASVLLRKLRWSTLGLQFDWSKR+Y++SLPHN IP ALCQLAK  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHG+PRIF  EE+ +I  L   LS++    F EYI+TSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIR 345

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 345

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BLAST of PI0003610 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.6e-45
Identity = 119/376 (31.65%), Postives = 193/376 (51.33%), Query Frame = 0


Query: 14  TAFRRAEKKYKLYYDDTYKSSKKKKLPKH----VDLSEVIDFKNILESYKQDGSLPVGV- 73
           T F R ++ ++       KS+  K +PK     +D S V+DF N+  + +++  L +   
Sbjct: 36  TEFMRVQRLFR----QVTKSANGKNIPKEKREPIDYSPVLDFHNLENNTEENKKLIIDCT 95

Query: 74  -NATKCDL-------------DGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQP 133
            N T  D              +  V+ L+  PGFYFI    +  +Q +WI+ +L D+  P
Sbjct: 96  SNVTTHDFEFNRDTEFYLHPREWKVYGLQGYPGFYFIKSPFTASQQKKWIKHALEDYADP 155

Query: 134 PNRTNHNAIYGPIQNLFIAAKGKKVLVEHDETS-----DFNLDSDVEPSISNGSTHNWKF 193
           PN  N    +GPI+NL+    G+K L+  +  S     D  ++    P   NG       
Sbjct: 156 PNNNNITLFHGPIKNLW--KNGEKELINEELKSQGKHDDDEIEQPTRPLDKNG------- 215

Query: 194 VEENTVSSRRGTACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAK 253
             E   + R+           LL KL WSTLG Q+ W+ R Y+    + + P  L +L +
Sbjct: 216 --EPLPTYRQ-----------LLDKLAWSTLGYQYQWTPRLYSEEF-YEEFPDDLQELVQ 275

Query: 254 RMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGK 313
           ++A A     + +  EAA VN+++    +GGHLDD E +  KPI+S+S G  A+FL+G +
Sbjct: 276 KIAIAT--KFDPYVAEAATVNFYSEDSIMGGHLDDAEQEMEKPIISISFGSTAVFLMGAE 335

Query: 314 SRQDPPVAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEY 364
           +R   PV +F+RSGD+V+M G +R C+HGV +I   E S ++  +  +  +QD  + +++
Sbjct: 336 TRDIAPVPLFIRSGDIVIMGGRSRYCYHGVAKIV--ENSFDLGLIDEN-DDQDLKYKIQW 379

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BLAST of PI0003610 vs. ExPASy Swiss-Prot
Match: Q13686 (Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-31
Identity = 93/297 (31.31%), Postives = 139/297 (46.80%), Query Frame = 0


Query: 80  FRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNHNAIYGPIQNLFIAAKGKKV 139
           + L+  PGF FIP       Q  W+++ L  + Q PN  N +                  
Sbjct: 91  YGLKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCNLDK----------------- 150

Query: 140 LVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRGTACKSIPASVLLRKLRWST 199
            +  +ET D                  W   E++    R   A K  P S LL KLRW T
Sbjct: 151 HMSKEETQDL-----------------W---EQSKEFLRYKEATKRRPRS-LLEKLRWVT 210

Query: 200 LGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLG 259
           +G  ++W  + Y+    +   P  L  L++++AAA     E+F+ EA I+NY+    TLG
Sbjct: 211 VGYHYNWDSKKYSAD-HYTPFPSDLGFLSEQVAAAC--GFEDFRAEAGILNYYRLDSTLG 270

Query: 260 GHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGV 319
            H+D  E D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H V
Sbjct: 271 IHVDRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAV 330

Query: 320 PRIFIDEESEEI--------------SFLARHLSNQDDSHFLEYIRTSRININIRQV 363
           PR+  + E E +                +    S +D      Y++T+R+N+ +RQV
Sbjct: 331 PRVLPNPEGEGLPHCLEAPLPAVLPRDSMVEPCSMEDWQVCASYLKTARVNMTVRQV 346

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BLAST of PI0003610 vs. ExPASy Swiss-Prot
Match: P0CB42 (Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 9.6e-30
Identity = 109/357 (30.53%), Postives = 160/357 (44.82%), Query Frame = 0


Query: 36  KKKLPKHVDLSEVIDFK--NILESYK----QDGSLPVGVNA-TKCDLD----GPV----- 95
           ++  P   DL  VIDF   ++  S K    Q    P+ V++ T+ D +     PV     
Sbjct: 31  RQSRPGTADLGAVIDFSEAHLARSPKPGVPQVVRFPLNVSSVTERDAERVGLEPVSKWRA 90

Query: 96  FRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNHNAIYGPIQNLFIAAKGKKV 155
           + LE  PGF FIP       Q  W+++ L  + Q PN                       
Sbjct: 91  YGLEGYPGFIFIPNPFLPGCQRHWVKQCLKLYSQKPNVC--------------------- 150

Query: 156 LVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRGTACKSIPASVLLRKLRWST 215
                     NLD  +    + G    W   E++    R     K  P S LL +LRW T
Sbjct: 151 ----------NLDKHMTKEETQGL---W---EQSKEVLRSKEVTKRRPRS-LLERLRWVT 210

Query: 216 LGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLG 275
           LG  ++W  + Y+    +   P  L  L++++A A    G  F+ EA I+NY+    TLG
Sbjct: 211 LGYHYNWDSKKYSAD-HYTPFPSDLAFLSEQVATACGFQG--FQAEAGILNYYRLDSTLG 270

Query: 276 GHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGV 335
            H+D  E D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H V
Sbjct: 271 IHVDRSELDHSKPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNHAV 330

Query: 336 PRIFIDEESEEI--------------SFLARHLSNQDDSHFLEYIRTSRININIRQV 363
           PR+    + E +              + L    S +D      Y+RT+R+N+ +RQV
Sbjct: 331 PRVLPHPDGECLPHCLETPLPAVLPSNSLVEPCSVEDWQVCATYLRTARVNMTVRQV 346

[Close]

BLAST of PI0003610 vs. ExPASy Swiss-Prot
Match: O60066 (Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=abh1 PE=2 SV=3)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 97/358 (27.09%), Postives = 154/358 (43.02%), Query Frame = 0


Query: 12  ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQDGSLPVGVNAT 71
           +   FR  EK+YK   D          +P   D+SEV+D  N  +S+        G  A 
Sbjct: 8   QANVFRLEEKRYKCRAD---------TIP---DMSEVLD-PNDPQSF--------GFEAL 67

Query: 72  KCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLM-DFPQPPNRTNHNAIY------ 131
             ++   VF  +  PG   +   +S + Q Q ++  +      P N+TN +  Y      
Sbjct: 68  -VEIKPRVFSFQKAPGLLILKNYVSSELQMQLLKSIMFTQIQDPENKTNLSPFYQLPLGN 127

Query: 132 GPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRGTACK 191
             I   +    G+ ++    ET    +D  V                             
Sbjct: 128 DSIWRRYYNGDGESIIDGLGETKPLTVDRLVH---------------------------- 187

Query: 192 SIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGEEFKP 251
                   +KLRW TLG Q+DW+ + Y         P  L    +++   +      +K 
Sbjct: 188 --------KKLRWVTLGEQYDWTTKEYPDPSKSPGFPKDLGDFVEKVVKESTDF-LHWKA 247

Query: 252 EAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGD 311
           EAAIVN+++ GDTL  H+D+ E D + P++S+S+G   I+L+G +SR + P A+ L SGD
Sbjct: 248 EAAIVNFYSPGDTLSAHIDESEEDLTLPLISLSMGLDCIYLIGTESRSEKPSALRLHSGD 298

Query: 312 VVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIRQV 363
           VV+M G +R+ FH VP+I     +   ++L       D      +I   R+N N+RQV
Sbjct: 308 VVIMTGTSRKAFHAVPKII---PNSTPNYLLTGNKAWDG-----WISRKRVNFNVRQV 298

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BLAST of PI0003610 vs. ExPASy TrEMBL
Match: A0A1S3BE32 (LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis melo OX=3656 GN=LOC103488884 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.2e-202
Identity = 350/363 (96.42%), Postives = 352/363 (96.97%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DGSLPVGV AT CDLD PVF LENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH
Sbjct: 61  DGSLPVGVKATTCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPIQ+LFIAAK KKVLVEHDE SDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           TACKSI ASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIP ALCQLAKRMAAAAMPTGE
Sbjct: 181 TACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKA FLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIFIDEESEEISFL RHLSNQDD HFLEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLERHLSNQDDLHFLEYIRTSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 363

[Close]

BLAST of PI0003610 vs. ExPASy TrEMBL
Match: A0A0A0KS00 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589370 PE=4 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 1.0e-199
Identity = 345/363 (95.04%), Postives = 351/363 (96.69%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESY+Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DGSLPVGVNAT CDLDGPVF LENRPGFYFIPGALSLQEQCQWIRESLM+FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPIQ+LFIAAK  KVLVEHDE SDF LDSDVEPSISNG+THNWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           TA KSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIP ALCQLAKRMAAAAMPTGE
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIFIDEESEEISFL  HL+NQDD H LEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 363

[Close]

BLAST of PI0003610 vs. ExPASy TrEMBL
Match: A0A6J1FZQ9 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=LOC111449397 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.4e-185
Identity = 321/363 (88.43%), Postives = 339/363 (93.39%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDD+ERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE Y Q
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DG+LP+GVNATKCDLDGPVF LENRPGFYFIPGALSL+EQCQWIRESL DFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPIQ+LFIAAK K+VLVE +E S  N+DSD EPS+ NG+++ WKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           T CKS+PAS LLRKLRWSTLGLQFDWSKRSY+ISLPHN+IP ALCQL KRMAAAAMPTGE
Sbjct: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIF DEESEEIS L +  S++DD HFLEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 362

[Close]

BLAST of PI0003610 vs. ExPASy TrEMBL
Match: A0A6J1HQB4 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LOC111466401 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 3.9e-183
Identity = 320/363 (88.15%), Postives = 337/363 (92.84%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE Y Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DG+LP+GVNATKCDLDGPVF LENRPGFYFIPGALSL+EQCQ IRESL DFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQRIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPIQ+LFIAAK K+VLVE +E S  N+DSD EPS+ NG+++ WKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           T CKS+PAS LLRKLRWSTLGLQFDWSKRSY+ISLPHN IP ALCQLAKRMAAAAMPTGE
Sbjct: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNDIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQD PVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDLPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIF DEESEEIS L +  S++DD HFLEY+RTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYMRTSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 362

[Close]

BLAST of PI0003610 vs. ExPASy TrEMBL
Match: A0A6J1DG90 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN=LOC111019811 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.6e-181
Identity = 314/363 (86.50%), Postives = 334/363 (92.01%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDD ERTAFR AEKKYK YYDDT+KSSKKKKLPK VDLSEVIDFK ILE Y  
Sbjct: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DG+LP+G+NAT+CDLDGPVF LENRPGFYFIPGALSL+EQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPIQ+LFIAAK K+VLVE +E S FN+DSD+EPS+S G+TH WKFVEENTVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRR- 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           T CKS+PASVLLRKLRWSTLGLQFDWSKRSY+ISL HNK+P ALCQLAKRMAAAAMP GE
Sbjct: 181 TTCKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGD+LGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIFID E++E S L +  SNQDD HFLEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDVETDETSLLEKQFSNQDDLHFLEYIRTSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 362

[Close]

BLAST of PI0003610 vs. NCBI nr
Match: XP_008446031.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [Cucumis melo])

HSP 1 Score: 714.5 bits (1843), Expect = 4.5e-202
Identity = 350/363 (96.42%), Postives = 352/363 (96.97%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DGSLPVGV AT CDLD PVF LENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH
Sbjct: 61  DGSLPVGVKATTCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPIQ+LFIAAK KKVLVEHDE SDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           TACKSI ASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIP ALCQLAKRMAAAAMPTGE
Sbjct: 181 TACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKA FLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIFIDEESEEISFL RHLSNQDD HFLEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLERHLSNQDDLHFLEYIRTSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 363

[Close]

BLAST of PI0003610 vs. NCBI nr
Match: XP_004135327.1 (alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Cucumis sativus])

HSP 1 Score: 705.7 bits (1820), Expect = 2.1e-199
Identity = 345/363 (95.04%), Postives = 351/363 (96.69%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESY+Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DGSLPVGVNAT CDLDGPVF LENRPGFYFIPGALSLQEQCQWIRESLM+FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPIQ+LFIAAK  KVLVEHDE SDF LDSDVEPSISNG+THNWKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           TA KSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIP ALCQLAKRMAAAAMPTGE
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIFIDEESEEISFL  HL+NQDD H LEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 363

[Close]

BLAST of PI0003610 vs. NCBI nr
Match: XP_038891364.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Benincasa hispida])

HSP 1 Score: 677.9 bits (1748), Expect = 4.7e-191
Identity = 332/363 (91.46%), Postives = 343/363 (94.49%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDK TDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK IL  YKQ
Sbjct: 1   MYGSDKVTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILGCYKQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DG+LP+GVNA KCDLD PVF LENRPGFYFIPGALSLQEQCQWIRESL +FPQP NRTNH
Sbjct: 61  DGALPLGVNAIKCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPSNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYG IQ+LFIAAKGKKVLVE DE SDFN+DSDVE S+SNG+THNWKFVEENTVSS+RG
Sbjct: 121 NAIYGSIQDLFIAAKGKKVLVEDDEISDFNVDSDVERSVSNGNTHNWKFVEENTVSSKRG 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           TACKSIPASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIP ALCQLAKRMAAAAMPTGE
Sbjct: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIFIDEESEEIS L RH SNQDD HFLEYI+ SRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISLLERHFSNQDDLHFLEYIKNSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 363

[Close]

BLAST of PI0003610 vs. NCBI nr
Match: XP_031740902.1 (alpha-ketoglutarate-dependent dioxygenase alkB isoform X2 [Cucumis sativus])

HSP 1 Score: 661.4 bits (1705), Expect = 4.6e-186
Identity = 331/364 (90.93%), Postives = 340/364 (93.41%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESY+Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIP-GALSLQEQCQWIRESLMDFPQPPNRTN 120
           DGSLPVGVNAT CDLDGPVF LENRPG    P   L L+++    RESLM+FPQPPNRTN
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGMADSPFSTLFLRKK----RESLMEFPQPPNRTN 120

Query: 121 HNAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRR 180
           HNAIYGPIQ+LFIAAK  KVLVEHDE SDF LDSDVEPSISNG+THNWKFVEENTVSSRR
Sbjct: 121 HNAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRR 180

Query: 181 GTACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTG 240
           GTA KSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIP ALCQLAKRMAAAAMPTG
Sbjct: 181 GTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTG 240

Query: 241 EEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMF 300
           EEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMF
Sbjct: 241 EEFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMF 300

Query: 301 LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININI 360
           LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFL  HL+NQDD H LEYIRTSRININI
Sbjct: 301 LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLETHLTNQDDLHCLEYIRTSRININI 360

Query: 361 RQVF 364
           RQVF
Sbjct: 361 RQVF 360

[Close]

BLAST of PI0003610 vs. NCBI nr
Match: XP_022945036.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata])

HSP 1 Score: 658.7 bits (1698), Expect = 3.0e-185
Identity = 321/363 (88.43%), Postives = 339/363 (93.39%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MYGSDKGTDD+ERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE Y Q
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DG+LP+GVNATKCDLDGPVF LENRPGFYFIPGALSL+EQCQWIRESL DFPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPIQ+LFIAAK K+VLVE +E S  N+DSD EPS+ NG+++ WKFVEENTVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           T CKS+PAS LLRKLRWSTLGLQFDWSKRSY+ISLPHN+IP ALCQL KRMAAAAMPTGE
Sbjct: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHGVPRIF DEESEEIS L +  S++DD HFLEYIRTSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGVPRIFTDEESEEISLLEKRFSSRDDLHFLEYIRTSRININIR 360

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 362

[Close]

BLAST of PI0003610 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.2e-138
Identity = 241/363 (66.39%), Postives = 280/363 (77.13%), Query Frame = 0


Query: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60
           MY S   +DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I +++  
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGSLPVGVNATKCDLDGPVFRLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120
           DG LP G+  +K D   PVF ++NRPGFYFIP ALSL+EQC+WI+ESL  FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIQNLFIAAKGKKVLVEHDETSDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180
           NAIYGPI +LF +AK  KVLV+ D T+                 + WKF EE  +     
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDLTN-----------------NKWKFYEEVDIEKATR 180

Query: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPPALCQLAKRMAAAAMPTGE 240
           ++CKS+ ASVLLRKLRWSTLGLQFDWSKR+Y++SLPHN IP ALCQLAK  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININIR 360
           RSGDVVLMAGEARECFHG+PRIF  EE+ +I  L   LS++    F EYI+TSRININIR
Sbjct: 301 RSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIR 345

Query: 361 QVF 364
           QVF
Sbjct: 361 QVF 345

[Close]

BLAST of PI0003610 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 2.3e-10
Identity = 42/122 (34.43%), Postives = 62/122 (50.82%), Query Frame = 0


Query: 244 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 303
           P+  IVN+++S   LG H D  E++ S     P+VS S+G  A FL G +  +D    + 
Sbjct: 346 PDICIVNFYSSTGRLGLHQDKDESENSIRKGLPVVSFSIGDSAEFLYGDQRDEDKAETLT 405

Query: 304 LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININI 362
           L SGDV+L  G +R+ FHGV  I  D   + +         Q+ S     +R  R+N+  
Sbjct: 406 LESGDVLLFGGRSRKVFHGVRSIRKDTAPKALL--------QETS-----LRPGRLNLTF 454

[Close]

BLAST of PI0003610 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 62.4 bits (150), Expect = 8.7e-10
Identity = 42/121 (34.71%), Postives = 58/121 (47.93%), Query Frame = 0


Query: 244 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 303
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 278 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 337

Query: 304 LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININI 361
           L SGDV++  GE+R  FHGV  I     S  +S L               +RT R+N+  
Sbjct: 338 LESGDVLIFGGESRMIFHGVKSII--PNSAPMSLLNE-----------SKLRTGRLNLTF 385

[Close]

BLAST of PI0003610 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 62.4 bits (150), Expect = 8.7e-10
Identity = 42/121 (34.71%), Postives = 58/121 (47.93%), Query Frame = 0


Query: 244 PEAAIVNYFASGDTLGGHLDDMEADWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 303
           P+  IVN+++    LG H D  E++ S     PIVS S+G  A FL G K   +    + 
Sbjct: 333 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 392

Query: 304 LRSGDVVLMAGEARECFHGVPRIFIDEESEEISFLARHLSNQDDSHFLEYIRTSRININI 361
           L SGDV++  GE+R  FHGV  I     S  +S L               +RT R+N+  
Sbjct: 393 LESGDVLIFGGESRMIFHGVKSII--PNSAPMSLLNE-----------SKLRTGRLNLTF 440

[Close]

BLAST of PI0003610 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 34/117 (29.06%), Postives = 48/117 (41.03%), Query Frame = 0


Query: 244 PEAAIVNYFASGDTLGGH----LDDMEADWSK---------------------PIVSMSL 303
           P+  +VN++ S   LG H     D    D+ K                     PIVS S+
Sbjct: 351 PDICVVNFYTSTGKLGLHQVSVYDKTSFDFLKYKGGYLNTDKGESKKSLRKGLPIVSFSI 410

Query: 304 GCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGV--------PRIFIDEE 328
           G  A FL G +   D    + L SGDV++    +R  FHGV        PR+F  ++
Sbjct: 411 GDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHGVRSIRKILPPRLFFRKQ 467

The following BLAST results are available for this feature:

BLAST of PI0003610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SA98	3.1e-137	66.39	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 G...	[more]
Q54N08	3.6e-45	31.65	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44...	[more]
Q13686	1.3e-31	31.31	Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2	[more]
P0CB42	9.6e-30	30.53	Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1	[more]
O60066	1.0e-26	27.09	Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (str...	[more]

BLAST of PI0003610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BE32	2.2e-202	96.42	LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis m...	[more]
A0A0A0KS00	1.0e-199	95.04	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A6J1FZQ9	1.4e-185	88.43	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1HQB4	3.9e-183	88.15	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1DG90	1.6e-181	86.50	alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN...	[more]

BLAST of PI0003610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446031.1	4.5e-202	96.42	PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [...	[more]
XP_004135327.1	2.1e-199	95.04	alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Cucumis sativus]	[more]
XP_038891364.1	4.7e-191	91.46	alpha-ketoglutarate-dependent dioxygenase alkB [Benincasa hispida]	[more]
XP_031740902.1	4.6e-186	90.93	alpha-ketoglutarate-dependent dioxygenase alkB isoform X2 [Cucumis sativus]	[more]
XP_022945036.1	3.0e-185	88.43	alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata]	[more]

BLAST of PI0003610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11780.1	2.2e-138	66.39	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]
AT3G14160.1	2.3e-10	34.43	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.1	8.7e-10	34.71	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.2	8.7e-10	34.71	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT3G14140.1	1.3e-05	29.06	2-oxoglutarate-dependent dioxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 188..360 e-value: 3.9E-40 score: 138.1
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 163..362 e-value: 8.9E-48 score: 164.6
IPR004574	Alkylated DNA repair protein AlkB	PANTHER	PTHR16557	ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED	coord: 1..363
None	No IPR available	PANTHER	PTHR16557:SF8	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB	coord: 1..363
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 86..362
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 243..363 score: 8.907888

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0003610.1	PI0003610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0035513	oxidative RNA demethylation
biological_process	GO:0035552	oxidative single-stranded DNA demethylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0035516	oxidative DNA demethylase activity
molecular_function	GO:0035515	oxidative RNA demethylase activity