PI0003310 (gene) Melon (PI 482460) v1

Overview
NamePI0003310
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionCystatin Hv-CPI6
Locationchr12: 20612577 .. 20613097 (+)
RNA-Seq ExpressionPI0003310
SyntenyPI0003310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAATACTACACATATAGATAAAACTATCCATATATATAAAATGATATTACAATATGAGTTGTGGGAAATCACTTTTTGGTGGTGCAACCCCAATTAAGGACATCAATGAACAAGTGGTAGAAGCTGGAAGGACAGCAGTGGACATCCATAACGGAGGAAACCAAGGCAAATTGACTTTCATTCGTGTGGTGAACGGTCTGGAAAGTGTTGTTAATGGCACCAAATATACTCTTGTGGTAGAGACTCTGAATGGTGATCAACTTTTTTGGACATTTAAGGTTACAATAGTGGTCAGTTATAATAATGTTTCAGGTAAATGCTATAAGCTCATATCTTGGGAAGATCCCCTCGGCTACTTCAAGTAGTATTCAAATATGTTATTTCTATCCATTTCATAAATACTGTCTTGTCTTCTATCCTTCTTTATAATAATAAATGGCATAGCTAGCCTTAAATACTACGTTCTAATTTTTTCTCTAATCTCTAATATAATCTCTGTCTTTTTAATTTCTCTTAAA

mRNA sequence

CAAAAATACTACACATATAGATAAAACTATCCATATATATAAAATGATATTACAATATGAGTTGTGGGAAATCACTTTTTGGTGGTGCAACCCCAATTAAGGACATCAATGAACAAGTGGTAGAAGCTGGAAGGACAGCAGTGGACATCCATAACGGAGGAAACCAAGGCAAATTGACTTTCATTCGTGTGGTGAACGGTCTGGAAAGTGTTGTTAATGGCACCAAATATACTCTTGTGGTAGAGACTCTGAATGGTGATCAACTTTTTTGGACATTTAAGGTTACAATAGTGGTCAGTTATAATAATGTTTCAGGTAAATGCTATAAGCTCATATCTTGGGAAGATCCCCTCGGCTACTTCAAGTAGTATTCAAATATGTTATTTCTATCCATTTCATAAATACTGTCTTGTCTTCTATCCTTCTTTATAATAATAAATGGCATAGCTAGCCTTAAATACTACGTTCTAATTTTTTCTCTAATCTCTAATATAATCTCTGTCTTTTTAATTTCTCTTAAA

Coding sequence (CDS)

ATGAGTTGTGGGAAATCACTTTTTGGTGGTGCAACCCCAATTAAGGACATCAATGAACAAGTGGTAGAAGCTGGAAGGACAGCAGTGGACATCCATAACGGAGGAAACCAAGGCAAATTGACTTTCATTCGTGTGGTGAACGGTCTGGAAAGTGTTGTTAATGGCACCAAATATACTCTTGTGGTAGAGACTCTGAATGGTGATCAACTTTTTTGGACATTTAAGGTTACAATAGTGGTCAGTTATAATAATGTTTCAGGTAAATGCTATAAGCTCATATCTTGGGAAGATCCCCTCGGCTACTTCAAGTAG

Protein sequence

MSCGKSLFGGATPIKDINEQVVEAGRTAVDIHNGGNQGKLTFIRVVNGLESVVNGTKYTLVVETLNGDQLFWTFKVTIVVSYNNVSGKCYKLISWEDPLGYFK
Homology
BLAST of PI0003310 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 1.2e-04
Identity = 24/72 (33.33%), Postives = 41/72 (56.94%), Query Frame = 0

Query: 9   GGATPIKDINE-QVVEAGRTAVDIHNGGNQGKLTFIRVVNGLESVVNGTKYTLVVETLNG 68
           G   PIK++++  VV   + A++ HN  ++ KL F++VV G   VV+GTKY L +   +G
Sbjct: 30  GSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDG 89

Query: 69  DQLFWTFKVTIV 80
                 ++  +V
Sbjct: 90  GGKIKNYEAVVV 101

BLAST of PI0003310 vs. ExPASy TrEMBL
Match: A0A5A7SX36 (Cystatin Hv-CPI6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold836G00250 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 7.3e-10
Identity = 43/100 (43.00%), Postives = 62/100 (62.00%), Query Frame = 0

Query: 10  GATPIKDINEQVVE-AGRTAVDIHNGGNQGKLTFIRVVNGLESVVN-GTKYTLVVETLNG 69
           GA PIKD+N+  V+ A   AV IHN      L FIRVVNG++  V+ G +Y+L++E  NG
Sbjct: 3   GAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEARNG 62

Query: 70  DQLFWTF--KVTIV---VSYNNVSGKCYKLISWEDPLGYF 103
           D   W +  K+ ++   VS+++     Y LISWEDPL ++
Sbjct: 63  DGTHWGYCAKIKLLRKPVSHHDPYPHSYVLISWEDPLKFY 102

BLAST of PI0003310 vs. ExPASy TrEMBL
Match: A0A0A0LVH4 (Cystatin Hv-CPI6 OS=Cucumis sativus OX=3659 GN=Csa_1G123470 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 8.0e-09
Identity = 42/101 (41.58%), Postives = 58/101 (57.43%), Query Frame = 0

Query: 9   GGATPIKDINEQVVE-AGRTAVDIHNGGNQGKLTFIRVVNGLESVVNGTK-YTLVVETLN 68
           G A PIKD+N+  V+ A   AV IHN      L FIRVVNGL+  V+  K Y+L++E  N
Sbjct: 2   GRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEATN 61

Query: 69  GDQLFWTFKVTIVV-----SYNNVSGKCYKLISWEDPLGYF 103
           GD   W +   I +     S+ +     ++LISWEDPL ++
Sbjct: 62  GDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFW 102

BLAST of PI0003310 vs. ExPASy TrEMBL
Match: A0A2N9F1M1 (Cystatin domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8895 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.6e-04
Identity = 40/101 (39.60%), Postives = 53/101 (52.48%), Query Frame = 0

Query: 4   GKSLFGGATPIKDINE-QVVEAGRTAVDIHNGGNQGKLTFIRVVNGLESVVNGTKYTLVV 63
           G S+ GG  PI+DI++  V E G  AVD +N  ++ +L+F+ VV G   VV+GT Y L V
Sbjct: 24  GGSVVGGWQPIQDISDPHVEEIGEYAVDEYNKESKSELSFVSVVKGETQVVSGTNYRLTV 83

Query: 64  ETLNGDQLFWTFKVTIVVSYNNVSGKCYKLISWEDPLGYFK 104
              +GD       VT     NN     Y+ I WE P   FK
Sbjct: 84  AAKDGD-------VT-----NN-----YEAIVWEKPWESFK 107

BLAST of PI0003310 vs. NCBI nr
Match: KAA0033827.1 (Cystatin Hv-CPI6 [Cucumis melo var. makuwa] >TYK21868.1 Cystatin Hv-CPI6 [Cucumis melo var. makuwa])

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-09
Identity = 43/100 (43.00%), Postives = 62/100 (62.00%), Query Frame = 0

Query: 10  GATPIKDINEQVVE-AGRTAVDIHNGGNQGKLTFIRVVNGLESVVN-GTKYTLVVETLNG 69
           GA PIKD+N+  V+ A   AV IHN      L FIRVVNG++  V+ G +Y+L++E  NG
Sbjct: 3   GAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEARNG 62

Query: 70  DQLFWTF--KVTIV---VSYNNVSGKCYKLISWEDPLGYF 103
           D   W +  K+ ++   VS+++     Y LISWEDPL ++
Sbjct: 63  DGTHWGYCAKIKLLRKPVSHHDPYPHSYVLISWEDPLKFY 102

BLAST of PI0003310 vs. NCBI nr
Match: KAE8645932.1 (hypothetical protein Csa_021380 [Cucumis sativus])

HSP 1 Score: 70.9 bits (172), Expect = 7.5e-09
Identity = 41/100 (41.00%), Postives = 59/100 (59.00%), Query Frame = 0

Query: 9   GGATPIKDINEQVVEAGRTAVDIHNGGNQGKLTFIRVVNGLE---SVVNGTK---YTLVV 68
           G   P  D  + V + GR AVDIHN      L F RV+NGLE   S ++G +   YTLVV
Sbjct: 7   GPPIPNIDQRKDVQQIGRIAVDIHNEQKPDTLIFHRVINGLEIGISDIHGPRILLYTLVV 66

Query: 69  ETLNGDQLFWTFKVTIVVSYNNVSGKCYKLISWEDPLGYF 103
           E +N D++ WT+  +I ++        Y+L+S++DPLGY+
Sbjct: 67  EAINKDKIIWTYVTSIKIT----KPSSYELVSFKDPLGYY 102

BLAST of PI0003310 vs. NCBI nr
Match: KGN64832.1 (hypothetical protein Csa_022861 [Cucumis sativus])

HSP 1 Score: 69.7 bits (169), Expect = 1.7e-08
Identity = 42/101 (41.58%), Postives = 58/101 (57.43%), Query Frame = 0

Query: 9   GGATPIKDINEQVVE-AGRTAVDIHNGGNQGKLTFIRVVNGLESVVNGTK-YTLVVETLN 68
           G A PIKD+N+  V+ A   AV IHN      L FIRVVNGL+  V+  K Y+L++E  N
Sbjct: 2   GRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEATN 61

Query: 69  GDQLFWTFKVTIVV-----SYNNVSGKCYKLISWEDPLGYF 103
           GD   W +   I +     S+ +     ++LISWEDPL ++
Sbjct: 62  GDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFW 102

BLAST of PI0003310 vs. NCBI nr
Match: KAE8645934.1 (hypothetical protein Csa_021370 [Cucumis sativus])

HSP 1 Score: 68.2 bits (165), Expect = 4.8e-08
Identity = 39/99 (39.39%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 9   GGATPIKDINEQVVEAGRTAVDIHNGGNQGKLTFIRVVNGLESVVNG-----TKYTLVVE 68
           G   P  D  + V + G  AVDIHN      L F RV+NGLE V+N        YTLVVE
Sbjct: 7   GSPIPNIDQRKDVQQIGGIAVDIHNEEKSDTLIFHRVINGLELVINDINGSILSYTLVVE 66

Query: 69  TLNGDQLFWTFKVTIVVSYNNVSGKCYKLISWEDPLGYF 103
            +N D+  WT+  +I ++        Y+L+ + DPLGY+
Sbjct: 67  AINKDKNIWTYVTSIKIT----KPSSYELVYFRDPLGYY 101

BLAST of PI0003310 vs. NCBI nr
Match: KAE8647444.1 (hypothetical protein Csa_003412 [Cucumis sativus])

HSP 1 Score: 68.2 bits (165), Expect = 4.8e-08
Identity = 39/99 (39.39%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 9   GGATPIKDINEQVVEAGRTAVDIHNGGNQGKLTFIRVVNGLESVVNG-----TKYTLVVE 68
           G   P  D  + V + G  AVDIHN      L F RV+NGLE V+N        YTLVVE
Sbjct: 7   GSPIPNIDQRKDVQQIGGIAVDIHNEEKSDTLIFHRVINGLELVINDINGSILSYTLVVE 66

Query: 69  TLNGDQLFWTFKVTIVVSYNNVSGKCYKLISWEDPLGYF 103
            +N D+  WT+  +I ++        Y+L+ + DPLGY+
Sbjct: 67  AINKDKNIWTYVTSIKIT----KPSSYELVYFRDPLGYY 101

BLAST of PI0003310 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 8.2e-06
Identity = 24/72 (33.33%), Postives = 41/72 (56.94%), Query Frame = 0

Query: 9   GGATPIKDINE-QVVEAGRTAVDIHNGGNQGKLTFIRVVNGLESVVNGTKYTLVVETLNG 68
           G   PIK++++  VV   + A++ HN  ++ KL F++VV G   VV+GTKY L +   +G
Sbjct: 30  GSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDG 89

Query: 69  DQLFWTFKVTIV 80
                 ++  +V
Sbjct: 90  GGKIKNYEAVVV 101

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84WT81.2e-0433.33Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A5A7SX367.3e-1043.00Cystatin Hv-CPI6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold836G002... [more]
A0A0A0LVH48.0e-0941.58Cystatin Hv-CPI6 OS=Cucumis sativus OX=3659 GN=Csa_1G123470 PE=4 SV=1[more]
A0A2N9F1M11.6e-0439.60Cystatin domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8895 ... [more]
Match NameE-valueIdentityDescription
KAA0033827.11.5e-0943.00Cystatin Hv-CPI6 [Cucumis melo var. makuwa] >TYK21868.1 Cystatin Hv-CPI6 [Cucumi... [more]
KAE8645932.17.5e-0941.00hypothetical protein Csa_021380 [Cucumis sativus][more]
KGN64832.11.7e-0841.58hypothetical protein Csa_022861 [Cucumis sativus][more]
KAE8645934.14.8e-0839.39hypothetical protein Csa_021370 [Cucumis sativus][more]
KAE8647444.14.8e-0839.39hypothetical protein Csa_003412 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G16500.18.2e-0633.33Cystatin/monellin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 14..78
e-value: 2.0E-9
score: 37.7
IPR000010Cystatin domainCDDcd00042CYcoord: 9..64
e-value: 1.41938E-6
score: 41.3056
NoneNo IPR availableGENE3D3.10.450.10coord: 3..103
e-value: 2.6E-13
score: 52.0
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 7..85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003310.1PI0003310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity