PI0003231 (gene) Melon (PI 482460) v1

Overview


Name	PI0003231
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Trypsin inhibitor 1
Location	chr04: 33362352 .. 33362788 (+)
RNA-Seq Expression	PI0003231
Synteny	PI0003231

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGTGAAAAAAAAACAAAAACAAAATAGTGAGATAACAACTACTCCAAGATGTCGCACCAGTGTAAGTATAACGCTTTTCGTAATATTTTGGTTGAGTTCAATATCTTCCTTTGTAAATCAAGCTGACACATTGTTTTTGTATGTTTCAAATTAGTAAAATCATGGCCAGAGCTGAATTTAATGGATGCAAACACTGTAGCAAATATCATCAAGAAAGAACACGCAAACTTTCAGATTGTCGTATTGCTGGCAGGATCTCCGGTAACATTAGATCTTCGACATGGCCGAGTTCGCCTCTTCGTCAATGTGCAGAATCGAGTAGTTCAGGTTCCTCGTGAGGGCTGAAATGTAACCACACACACACACACATATATATATATATATTTCACATAATAAATATTAAATAAAGTAGAAACTATTTCTACTACTTTGCATA

mRNA sequence

TTGTGAAAAAAAAACAAAAACAAAATAGTGAGATAACAACTACTCCAAGATGTCGCACCAGTTAAAATCATGGCCAGAGCTGAATTTAATGGATGCAAACACTGTAGCAAATATCATCAAGAAAGAACACGCAAACTTTCAGATTGTCGTATTGCTGGCAGGATCTCCGGTAACATTAGATCTTCGACATGGCCGAGTTCGCCTCTTCGTCAATGTGCAGAATCGAGTAGTTCAGGTTCCTCGTGAGGGCTGAAATGTAACCACACACACACACACATATATATATATATATTTCACATAATAAATATTAAATAAAGTAGAAACTATTTCTACTACTTTGCATA

Coding sequence (CDS)

ATGTCGCACCAGTTAAAATCATGGCCAGAGCTGAATTTAATGGATGCAAACACTGTAGCAAATATCATCAAGAAAGAACACGCAAACTTTCAGATTGTCGTATTGCTGGCAGGATCTCCGGTAACATTAGATCTTCGACATGGCCGAGTTCGCCTCTTCGTCAATGTGCAGAATCGAGTAGTTCAGGTTCCTCGTGAGGGCTGA

Protein sequence

MSHQLKSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPREG

Homology

[Close]

BLAST of PI0003231 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.6e-07
Identity = 31/63 (49.21%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 6  KSWPELNLMDANTVANIIKKEHANF-QIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVP 65
          ++WPEL  + A     II+KE++    +  +L GSPVT D R  RVRLFVNV + VVQ+P
Sbjct: 32 ETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQIP 91

Query: 66 REG 68
          R G
Sbjct: 92 RVG 94

[Close]

BLAST of PI0003231 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 6.2e-07
Identity = 31/59 (52.54%), Postives = 38/59 (64.41%), Query Frame = 0


Query: 8   WPELNLMDANTVANIIKKEHANF-QIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPR 66
           WPEL  + A     II+KE+ +   I +LL GSPVT D R  RVRLFVN+   VVQ+PR
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIPR 109

[Close]

BLAST of PI0003231 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 1.4e-06
Identity = 27/61 (44.26%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 7  SWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPRE 66
          SWPEL   + +  A II+ E+AN + +V+  G P+T DL   RVR+FV+    V QVP  
Sbjct: 10 SWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQVPAI 69

Query: 67 G 68
          G
Sbjct: 70 G 70

[Close]

BLAST of PI0003231 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 3.1e-06
Identity = 28/59 (47.46%), Postives = 37/59 (62.71%), Query Frame = 0


Query: 8   WPELNLMDANTVANIIKKEHANF-QIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPR 66
           WPEL  +       II+KE++    + +LL GSPVT+D R  RVRLF N+   VVQ+PR
Sbjct: 47  WPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNILGDVVQIPR 105

[Close]

BLAST of PI0003231 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.0e-06
Identity = 30/61 (49.18%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 8   WPELNLMDANTVANIIKKEHANF-QIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPRE 67
           WPEL    A     II+KE+     +  +L G+PVT DLR  RVRLFVNV + VVQ P+ 
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQTPQV 106

[Close]

BLAST of PI0003231 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 2.8e-18
Identity = 48/65 (73.85%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 3  HQLKSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQ 62
          HQLKSWP LN MDA TVANIIKKE+ +FQI +LLAGSPVT DL+HGRVRLF N++  VV 
Sbjct: 7  HQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVI 66

Query: 63 VPREG 68
          VP+EG
Sbjct: 67 VPQEG 71

[Close]

BLAST of PI0003231 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.6e-10
Identity = 36/63 (57.14%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 5  LKSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVP 64
          LKSWP L  +DA TVAN IK E+ NF+IV +  G+PVT D+  GRVRL V+   +VV VP
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 65 REG 68
          +EG
Sbjct: 67 QEG 69

[Close]

BLAST of PI0003231 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.4e-08
Identity = 32/61 (52.46%), Postives = 45/61 (73.77%), Query Frame = 0


Query: 7  SWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPRE 66
          SWPEL  + ++   + I+++  + + VVLLAGSPVT DLR  RVR+FVN+ +RVV+VPR 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 67 G 68
          G
Sbjct: 75 G 75

[Close]

BLAST of PI0003231 vs. ExPASy TrEMBL
Match: A0A6J1CY53 (inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=LOC111015335 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.9e-07
Identity = 32/62 (51.61%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 6  KSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPR 65
          + WPEL  +  +  A IIK+E+A  +  VLLAGSPVTLD R  RVR+F N+++ VV +PR
Sbjct: 18 QEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPR 77

Query: 66 EG 68
           G
Sbjct: 78 TG 79

[Close]

BLAST of PI0003231 vs. ExPASy TrEMBL
Match: A0A397ZK38 (Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 3.2e-06
Identity = 30/63 (47.62%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 5  LKSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVP 64
          LK+WPEL     +  A++IK+E+ +F + VLL GS VT DLR  RVR++V+    VV+VP
Sbjct: 3  LKTWPELVGTHGDDAASVIKRENPSFNVFVLLIGSIVTADLRENRVRVWVDTNRIVVRVP 62

Query: 65 REG 68
            G
Sbjct: 63 TTG 65

[Close]

BLAST of PI0003231 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 103.6 bits (257), Expect = 6.8e-19
Identity = 48/67 (71.64%), Postives = 58/67 (86.57%), Query Frame = 0


Query: 1  MSHQLKSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRV 60
          M++ LKSWPELNLM A+TV NIIK++H  FQIV+LLAGSPVT DL+HGRVRLFVN+   V
Sbjct: 1  MAYVLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIV 60

Query: 61 VQVPREG 68
          V++PREG
Sbjct: 61 VEIPREG 67

[Close]

BLAST of PI0003231 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 100.5 bits (249), Expect = 5.7e-18
Identity = 48/65 (73.85%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 3  HQLKSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQ 62
          HQLKSWP LN MDA TVANIIKKE+ +FQI +LLAGSPVT DL+HGRVRLF N++  VV 
Sbjct: 7  HQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVI 66

Query: 63 VPREG 68
          VP+EG
Sbjct: 67 VPQEG 71

[Close]

BLAST of PI0003231 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 74.7 bits (182), Expect = 3.4e-10
Identity = 36/63 (57.14%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 5  LKSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVP 64
          LKSWP L  +DA TVAN IK E+ NF+IV +  G+PVT D+  GRVRL V+   +VV VP
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 65 REG 68
          +EG
Sbjct: 67 QEG 69

[Close]

BLAST of PI0003231 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 66.6 bits (161), Expect = 9.2e-08
Identity = 32/61 (52.46%), Postives = 45/61 (73.77%), Query Frame = 0


Query: 7  SWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPRE 66
          SWPEL  + ++   + I+++  + + VVLLAGSPVT DLR  RVR+FVN+ +RVV+VPR 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 67 G 68
          G
Sbjct: 75 G 75

[Close]

BLAST of PI0003231 vs. NCBI nr
Match: XP_022146026.1 (inhibitor of trypsin and hageman factor-like [Momordica charantia])

HSP 1 Score: 63.9 bits (154), Expect = 5.9e-07
Identity = 32/62 (51.61%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 6  KSWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPR 65
          + WPEL  +  +  A IIK+E+A  +  VLLAGSPVTLD R  RVR+F N+++ VV +PR
Sbjct: 18 QEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPR 77

Query: 66 EG 68
           G
Sbjct: 78 TG 79

[Close]

BLAST of PI0003231 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.3e-07
Identity = 26/61 (42.62%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 7  SWPELNLMDANTVANIIKKEHANFQIVVLLAGSPVTLDLRHGRVRLFVNVQNRVVQVPRE 66
          SWPEL   + +  A +I++E+      V+L GSPVT D R  RVR+FV+    VV+ P+ 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 67 G 68
          G
Sbjct: 70 G 70

The following BLAST results are available for this feature:

BLAST of PI0003231 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q02214	3.6e-07	49.21	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P16231	6.2e-07	52.54	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
Q6XNP7	1.4e-06	44.26	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P08454	3.1e-06	47.46	Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2	[more]
Q03199	4.0e-06	49.18	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]

BLAST of PI0003231 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	2.8e-18	73.85	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0LFD4	1.6e-10	57.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1	[more]
A0A0A0KPI0	4.4e-08	52.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A6J1CY53	2.9e-07	51.61	inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A397ZK38	3.2e-06	47.62	Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1	[more]

BLAST of PI0003231 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571807.1	6.8e-19	71.64	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60477.1	5.7e-18	73.85	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
KGN60478.1	3.4e-10	57.14	hypothetical protein Csa_001396 [Cucumis sativus]	[more]
XP_011654473.1	9.2e-08	52.46	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
XP_022146026.1	5.9e-07	51.61	inhibitor of trypsin and hageman factor-like [Momordica charantia]	[more]

BLAST of PI0003231 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	1.3e-07	42.62	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 7..20 score: 32.92 coord: 44..54 score: 60.08 coord: 32..43 score: 50.0
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 7..66 e-value: 1.2E-15 score: 57.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 4..67
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 2..67 e-value: 2.9E-17 score: 64.6
None	No IPR available	PANTHER	PTHR33091:SF69	SUBFAMILY NOT NAMED	coord: 4..67
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 3..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0003231.1	PI0003231.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity