PI0003187 (gene) Melon (PI 482460) v1

Overview
NamePI0003187
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionmitochondrial import inner membrane translocase subunit TIM50
Locationchr01: 13148126 .. 13154144 (-)
RNA-Seq ExpressionPI0003187
SyntenyPI0003187
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTTTTTGCTTTCGGGTGAGTTATTTTTTCCTTCTCCTTCATGTATTTTTTTCTTTTTAATAACTGCCTCCGAAATGGAAATATGCAGCCCAAGAAGCAGCTTCCAGAACCTCGTCCAGAGTACCATACACCGACTTCCAAACAGTCCCCCCTCTAACTTCAAACCATTCCTCTCTCTTTGCCAAAACCCTACTTTCAAACTTGAAAAAAACAAACAAAATTATAACACAAAATTGATCTAAACCCTAATCTTCCCAAAATGTCCCCTCTCATTTTCCGATCCCGATTCATTTCCCTTCTGAAGAATCGCCATGGCCGTCTTATGAGCTCCGACCTCGCTTCAACCCCTTCCAAAGATGCCATCAATGCCTCTCAGAAGCTCGTCTCCGATCAGATTCCACCTGATGCTCAAACCACTTCTGCTACTTCTTCTTCTTCCTCTAGTAAGCCCTGGAATTTCTTCAAGTATAGTTTGATTGGTGCTCTCACAGGTGCAACTGCGGTTGCTGGTTACGCCAGTTATGGTATTTTATCCCTTCCTTCTTTATAACTCCTTATCTCTCGTTTAGCATGATGCTATTTGATCTGGTTTCTATTTGATTTTATTTCATGAATGGATTGGAGTTACGTGGTGGTGATTTGTTTTTCAGCCTATAGTTATGATGAAATTGAGGAGAAGGCGAAAGCTTTACGTCGTTCTGCTAGTCATACTGCCACGGACGATGTCTCAGGGGTTGATGTAAGTATGAGGATGAACCGAACTCAAGTTCTTTTTTTTTTTTTTTTTTTTTTTATCTTGATTGTTTTCTTTATCGGATTGGCTCTTTTTCTTTCCAATGTACCTACTCAAGCATGATCGAGTTTTGTAACAACATAAGTGAACTGGTACGTGCCTGTTTATTGGGTTTTCTGGGATGGTGCAATTCCAAATTGATTTTCATTAGTCGTGGTTGAAATTATTTTGAGTTGTTTGTGTCAAATGAGGTTGCGTGCTTATGTATGTTTCTTCCCCGTCTCTTTATACCAGCTTCAGTTGCTTCTTGGTGCGAAGCATAACTTTCCGCCATTGTTTTTCTTTTGCAGAAAGTTCAGGGCATGCTCTACTCCACGATAATGACAGGTAGAAGAACTCATCTTTCTTTTCTTGTTGACTTTTCCAATTCAAACATTTACTCGTTGCATGATCTTAATCTTAAGATTAAGACCATTTACAGTTTGTTGTTTGGATAACTTCTTTAAATAAGAAACGTTTCATTGACCAATGAAATGAGGGGAAAACCCGTACACCTATAGGTGACTATAAGTTTACAATAAATATTTCCAATTGGAAACTAAATAAGATAAACTTGAAAACCCTGCTGGCTATAGAGCATAATAACTCTTTGGAACTTATTTAGATTGTAGAAAATAAGTTAGGTTAGAGTCTTCAGATGAAGGATTATGGTTTGGGGAGGGCAATTAAAGAATTTGCTGTTGGGGGGCTGAGGTTTCCACTTTCCACTGGAGAGCAGTAGCATAGCGCAGAGAGAGACCGAAGTTCATCTCACTTTATCTTTTAACTGTTATAATTATAAGCCCTAAATTATATCTTATCAGCTAAGAAATGTGAATATAAAATGTGTGCCTTGATGACTCAGCTGCTGGGCCTGGTCCCAAAGTGCATCCTTAGGTGATGCTTGATTGAGTGTGCTCTTGCCTTCACTAAGGTGCCTTTGATGTTTTGTGCACCTATGCACATTTTTCAAATGCTGCTCCTCTGACTTAACAAAGTAAAGATTTGCAAATATATTGTAGACATTGCTACTTACTCTTTTATGCTTATTATATGGCGACGTTAACTTGTAGAATCATAGTTTCTTTAGTTGACGAGAAGAACCTTTTGTTGCAGTTCCTGCAAAACTTGTTGACGTTTACCTTGATCTGAGGAAGACAATTGAAGAACAAGTTAAAGTGCGTATGTTGTTTCAGTTTAACATTGCTCTTGGATGTTTATCAACCTCAGTAGATTCACTTATTTCTGACCATAAGTTTCTTGCTCATTGTAGGGTTATACTGAACCCAATGCGGAAATGCTTCTTCCAGATTTGCATCCCATGGAACGACACGTGTTCACGCTTGTTTTAGATCTAAATGAGACTCTTGTGTACTCTGACTGGACGGTAAGCCTAATATTGAAGATAATGTTGAGTTCTTGTGCACTCGTAATTTTTTGATTTCATCATTTTGGGTGCTTCATGTTGTTGTTTTGGCCTTTTCTGCATGGCTTCCTTTTTGTTCATATCTACTTTCTTCTTTGTGAGCTGGTTTCTTAGCCAAAAAGCTGTTATTTGTAATTAGTGAATTTGTTAAATTGTATTTTGACTCTTGCCAAAAAAGAAAGCATTTTGTCTAGCTTTTTCACCTGTGTTTCATTTTTATTTTCTTTTCATCGTGTTTCTTTTTTGATTTTTTATCAATAAACTCTTCTTAAATATCATTGGATTTAAGATGTTTATCTTTTATGCTTGTGCTTGATGATAACATAGACTTGTTGGGTATCTTTTCATTTGTTGTAACAACTTGTTCTTTTTGTTTACAAAGAGAGAAAGAGGCTGGCAGACGTTTAAAAGACCTGGAGTGGACTCTTTTTTGGAACATCTTGCCCAGTTTTATGAAATTGTTATATACTCTGATCAATCAAATATGGTAATCTGTTGTCTTTTCATCTTAACATTGATTGCCGCATTTAATCACTACAAAGTTCTAATTTATTTTGTTTTGCTATTGCAGTATGTTGATCCTGTTATTGAAAGGTTGGATCCCAAGCATTGCATACGATATAGGTTGTCAAGAGCAGCTACGAAGTATGAGAATGGAAAGCATTACAGGGTATGTTGTGGATGAGAGTTTATTTCTTTTTTTTTCTCTCTGATAAATTCTTCCCCTCTTTTTTGGATCTTACGATTTTGCATTCACGTGAGTCTTGAAGATGATGATAAGTTGTTTAAAGCTAGCTCATGACATGAAATTTACCATTCCACGTTGGGTGCTAGCTGGGCTGCACCCAGCTTTTGCAACCCATCCACCTGGCAAGCTCCCATTTTGCTTCTTTTTCTTACATATATACGTTTTTAATTCTCCTTTTACTCCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTCATTTTTTTCTGTTTTTTTTCTGTTTTTTTTTTCATTTATTTTCTTGTACTATGTGGGTGGGAGAAGAAAATAAAAAGATTTATACAAAATATAAAATAAGAAATGAAATTGATTGTGGAGCCTCTATTAGCTTTAGGTCATGAAACTATTTTCCACACTGTTATTTTAACGTCACAAATTTTATCTATTAATTGAAAGTAAATGATCTAGTGTATATGAGAAAATAAAATGTGAATAAATAAATACTATAAAATTAATAATGATATTGATATTATCAAAGAGTTTGTAAATAAGAAAAAATGATCTTTTCTCGGCTGCAAAAGGCTACACCCAAGTAAAATGAGGTGGGATCCTCTACCAGTAAATATAATTTACGATTTTATCATTTTAGGAAGGCAAAGTTAAAGTGTGAACTGACTGGAAATCTCCCCCCCAACAAGTTTTGTCCTTCTACATTACATTACTTATTAGTTCTCTGCACGTGTCATAGAGAACTTTTTGTAATTCCTTTGGATGAAAAGGATGAAAAATTGTAAATGGAAAATAAGGTGATCAGTTTTATTATTAATATAGTAATACAGTAAGGTAACACATAAATTATAAATTTTATTCAATTATATAATGCACCTTTTTGTTGACAGGACTTGTCAAAGCTTAACAGGGATCCGAGGAAGATTATTTATTTAAGTGGCCATGCATTTGACAGTAGTCTACAACCAGAAAATTCTGTTCCAATAAAGCCATGGAAGTGTGAGACAGATGACACAGCTCTTCTTGATTTTATACCATTTCTTGAATGTAAGTATTCTGATATTGCAATGACTATGATGAGAATTCATAACTAGGGATGATTTTGTTAGGTTGGTATAAATTTGTTTTCTGGTCACGAAGGCATTTACTTGATTATGCAGTTGTTGCACGAAATAGTCCAGCCGACATCCGACAAGTACTAGAATCGTATAAAGGATGTGATATTCCAACAGAATTTATAAGACGTTCTAAAGAGCATCAAAGGTGATTCTCTTCTCTCTTTTTTTTAAACCTTTGCAACTGTTGCATTTTTACTCTCTTGGTCTCTTTCAATTTGAATTATAATTCTAGTTTACTTTGTTATTCTCAATGTGTAGGCCCCGTCTTTCCTTCTTCCTTTCTTTTATTTTAATCCTTGTGCTTTTATAAATAGAACTTAATATCTGACATTAATTATAGTTCATTAATAGAAGAAATTGACCTGCCATGGTGTCTCGTCAGTCTTGACTTTTTTGTTAGCATTTAAGGATAATTGGCATTGGAGACCAAAAGGTTAAAAACTAAATATATTAAAGCTTTGGAACCAAATATCTTTCTGGTTGGTTTTTTATTTTCAGAAACTTGTGTCCTTGAACTTTTAACTTCCTTTTCATCTATGCATGAAAATTGTTTCTAGTCAAAAGAAAAATGAAAAAAAAAAAAGAATAGAAAAGGCCATATAGTTCAAAACGAAAAATTACGAGGACTAGAATGATAATCAACCCTTTTCCTTGATCATCGATTGCTTCGGGGTTGCCTTTGTTCAAATTCTTTCCTCCTTAAGCTTTTGATACCAAAGGCAGTGTATGCAAAGGAAATAGTTCCATTGTCACTTATGATTTATATTTGTTGTTGGACATTCGTAGATTTGCGTGATTGATGAGAATACCACTTGTCTAGATTATTGTTTTATAAGTATACTTACCTGATCCATTTGGAGGCTTAATTTACTATTTTTGCTTTAATTTTAGGGGCATTCTAGTATGCTTGTAAAGTTGGACTATTTTTTATTAAAAGACATCTAACAGAGTCGATTAAAAATGCCTTTGGAAAAAGTTGACTAAAAATGCTTGCGAAGTAGAAAAGATGGATATATACATATTGTCAGATGAAATCAAATTAGATAACCAATTGATTTATCTTAAAAAAAGAAAGTAAGGGAAAGGACCAATTAGTTTCTGTGATGGACCACTTGGACCTGTTGGTTATTAAAGCAAGTGGAAAAAAGTTAAAGAGGGATTGAAGAATGGTTCAAACCCTAAATGTCACCTACCATAGATAGTTCTTAGTAAAGGGACTTAACAACTCCTCATACTTAAGTCCACTCCTTGTCCCAAATGCTCCAAAAGGATAAGGAAAATTAGTGATTTTTTTTGTCCTTATATCATTTGTTGCATCCATCTTATGATTTCAACTATTGACATATCAGTACTTCAAATGATCTAAATCTCATTCTCACCTAAGAATCAATAATCTTAACTAATGATGCAGGCGGATTCAAGAACAGAAGCAGCAGGGGCGAATATGGAAACGTTGAGATCAAAATATAGTGTAAAAAGTTGATTGAAGATGTTGTCAAACAAAAACAAAGAATATTGTTTAGAATCAACTGGAGCCAACTCTCTTCGTGCAGGCCAAGAAGCAACTTCAGATTGATTCCATCGATTTTCTCTTCTACATTCCATTAGTTGCAGCTTCTTGATAAGCAAGAGCAGTACGTCACTTTTTCTTTTTGTCTCACTCAATTTGAATAACCCCCCTCCCCCATAATTTTGCAACTTATTTGTCCCATCATTTCTGGAGAAGAGATTGTTCTGCCAGGCAATGGAGTTTTGATTGTGTGTCATTTAGCTCCTTACTGAGTATTCCTGAATAGCTATTACTGTCCAAGTTTAGTTACAATTATTAACCCTTTGATTTTGTTTTCTTTTCTTTTAAAAAAATTTATATTTGTATAGATCAATATTATATCAAATTAGAGTAGTTGCCTCACTTTACTGGTTTTTTTTAATTTTATTTGACTTCATCGTTACCTTCATATGACAATGTAATATATTCTTGTTTTGAA

mRNA sequence

ATTTTTTTTTGCTTTCGGGTGAGTTATTTTTTCCTTCTCCTTCATGTATTTTTTTCTTTTTAATAACTGCCTCCGAAATGGAAATATGCAGCCCAAGAAGCAGCTTCCAGAACCTCGTCCAGAGTACCATACACCGACTTCCAAACAGTCCCCCCTCTAACTTCAAACCATTCCTCTCTCTTTGCCAAAACCCTACTTTCAAACTTGAAAAAAACAAACAAAATTATAACACAAAATTGATCTAAACCCTAATCTTCCCAAAATGTCCCCTCTCATTTTCCGATCCCGATTCATTTCCCTTCTGAAGAATCGCCATGGCCGTCTTATGAGCTCCGACCTCGCTTCAACCCCTTCCAAAGATGCCATCAATGCCTCTCAGAAGCTCGTCTCCGATCAGATTCCACCTGATGCTCAAACCACTTCTGCTACTTCTTCTTCTTCCTCTAGTAAGCCCTGGAATTTCTTCAAGTATAGTTTGATTGGTGCTCTCACAGGTGCAACTGCGGTTGCTGGTTACGCCAGTTATGCCTATAGTTATGATGAAATTGAGGAGAAGGCGAAAGCTTTACGTCGTTCTGCTAGTCATACTGCCACGGACGATGTCTCAGGGGTTGATAAAGTTCAGGGCATGCTCTACTCCACGATAATGACAGTTCCTGCAAAACTTGTTGACGTTTACCTTGATCTGAGGAAGACAATTGAAGAACAAGTTAAAGGTTATACTGAACCCAATGCGGAAATGCTTCTTCCAGATTTGCATCCCATGGAACGACACGTGTTCACGCTTGTTTTAGATCTAAATGAGACTCTTGTGTACTCTGACTGGACGAGAGAAAGAGGCTGGCAGACGTTTAAAAGACCTGGAGTGGACTCTTTTTTGGAACATCTTGCCCAGTTTTATGAAATTGTTATATACTCTGATCAATCAAATATGGTAATCTGTTGTCTTTTCATCTTAACATTGATTGCCGCATTTAATCACTACAAAGTTCTAATTTATTTTGTTTTGCTATTGCAGTATGTTGATCCTGTTATTGAAAGGTTGGATCCCAAGCATTGCATACGATATAGGTTGTCAAGAGCAGCTACGAAGTATGAGAATGGAAAGCATTACAGGGACTTGTCAAAGCTTAACAGGGATCCGAGGAAGATTATTTATTTAAGTGGCCATGCATTTGACAGTAGTCTACAACCAGAAAATTCTGTTCCAATAAAGCCATGGAAGTGTGAGACAGATGACACAGCTCTTCTTGATTTTATACCATTTCTTGAATTTGTTGCACGAAATAGTCCAGCCGACATCCGACAAGTACTAGAATCGTATAAAGGATGTGATATTCCAACAGAATTTATAAGACGTTCTAAAGAGCATCAAAGGCGGATTCAAGAACAGAAGCAGCAGGGGCGAATATGGAAACGTTGAGATCAAAATATAGTGTAAAAAGTTGATTGAAGATGTTGTCAAACAAAAACAAAGAATATTGTTTAGAATCAACTGGAGCCAACTCTCTTCGTGCAGGCCAAGAAGCAACTTCAGATTGATTCCATCGATTTTCTCTTCTACATTCCATTAGTTGCAGCTTCTTGATAAGCAAGAGCAGTACGTCACTTTTTCTTTTTGTCTCACTCAATTTGAATAACCCCCCTCCCCCATAATTTTGCAACTTATTTGTCCCATCATTTCTGGAGAAGAGATTGTTCTGCCAGGCAATGGAGTTTTGATTGTGTGTCATTTAGCTCCTTACTGAGTATTCCTGAATAGCTATTACTGTCCAAGTTTAGTTACAATTATTAACCCTTTGATTTTGTTTTCTTTTCTTTTAAAAAAATTTATATTTGTATAGATCAATATTATATCAAATTAGAGTAGTTGCCTCACTTTACTGGTTTTTTTTAATTTTATTTGACTTCATCGTTACCTTCATATGACAATGTAATATATTCTTGTTTTGAA

Coding sequence (CDS)

ATGTCCCCTCTCATTTTCCGATCCCGATTCATTTCCCTTCTGAAGAATCGCCATGGCCGTCTTATGAGCTCCGACCTCGCTTCAACCCCTTCCAAAGATGCCATCAATGCCTCTCAGAAGCTCGTCTCCGATCAGATTCCACCTGATGCTCAAACCACTTCTGCTACTTCTTCTTCTTCCTCTAGTAAGCCCTGGAATTTCTTCAAGTATAGTTTGATTGGTGCTCTCACAGGTGCAACTGCGGTTGCTGGTTACGCCAGTTATGCCTATAGTTATGATGAAATTGAGGAGAAGGCGAAAGCTTTACGTCGTTCTGCTAGTCATACTGCCACGGACGATGTCTCAGGGGTTGATAAAGTTCAGGGCATGCTCTACTCCACGATAATGACAGTTCCTGCAAAACTTGTTGACGTTTACCTTGATCTGAGGAAGACAATTGAAGAACAAGTTAAAGGTTATACTGAACCCAATGCGGAAATGCTTCTTCCAGATTTGCATCCCATGGAACGACACGTGTTCACGCTTGTTTTAGATCTAAATGAGACTCTTGTGTACTCTGACTGGACGAGAGAAAGAGGCTGGCAGACGTTTAAAAGACCTGGAGTGGACTCTTTTTTGGAACATCTTGCCCAGTTTTATGAAATTGTTATATACTCTGATCAATCAAATATGGTAATCTGTTGTCTTTTCATCTTAACATTGATTGCCGCATTTAATCACTACAAAGTTCTAATTTATTTTGTTTTGCTATTGCAGTATGTTGATCCTGTTATTGAAAGGTTGGATCCCAAGCATTGCATACGATATAGGTTGTCAAGAGCAGCTACGAAGTATGAGAATGGAAAGCATTACAGGGACTTGTCAAAGCTTAACAGGGATCCGAGGAAGATTATTTATTTAAGTGGCCATGCATTTGACAGTAGTCTACAACCAGAAAATTCTGTTCCAATAAAGCCATGGAAGTGTGAGACAGATGACACAGCTCTTCTTGATTTTATACCATTTCTTGAATTTGTTGCACGAAATAGTCCAGCCGACATCCGACAAGTACTAGAATCGTATAAAGGATGTGATATTCCAACAGAATTTATAAGACGTTCTAAAGAGCATCAAAGGCGGATTCAAGAACAGAAGCAGCAGGGGCGAATATGGAAACGTTGA

Protein sequence

MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSATSSSSSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDKVQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDIPTEFIRRSKEHQRRIQEQKQQGRIWKR
Homology
BLAST of PI0003187 vs. ExPASy Swiss-Prot
Match: Q8VYE2 (Mitochondrial import inner membrane translocase subunit TIM50 OS=Arabidopsis thaliana OX=3702 GN=TIM50 PE=1 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.2e-108
Identity = 214/404 (52.97%), Postives = 273/404 (67.57%), Query Frame = 0

Query: 1   MSPLIFRSRF---ISLLKNRHGRLMSSDLASTPSK---DAINASQKLVSD---------Q 60
           M+ ++ RSR    ++ L++R+ R  S++ +ST S      + ++Q + SD          
Sbjct: 1   MASIVLRSRLLPRLAKLRSRNLRCFSAEASSTNSTSRYSGVTSTQSMFSDFPPPNQPPPP 60

Query: 61  IPPDAQTTSATSSSSSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRS 120
            PP  +  +A ++    K   +  Y+L+ ALTGATA  GYAS+AY+ DE+ EK KA R S
Sbjct: 61  PPPQVEAAAAAATGKERKGLKYLGYALLWALTGATAATGYASFAYTIDEVNEKTKAFRES 120

Query: 121 ASHTATDDVSG---VDKVQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLL 180
           A+ T     SG   +DK Q  LYS  MT  A+ +D YL+LR+ +EEQVKG+TEP +E LL
Sbjct: 121 ATKTPVIKSSGIDVIDKYQTKLYSAAMTGSARAIDKYLELREIVEEQVKGFTEPLSEKLL 180

Query: 181 PDLHPMERHVFTLVLDLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQS 240
           PDLHP E+HVFTLVLDLNETL+Y+DW RERGW+TFKRPGVD+FLEHL +FYEIV+YSDQ 
Sbjct: 181 PDLHPAEQHVFTLVLDLNETLLYTDWKRERGWRTFKRPGVDAFLEHLGKFYEIVVYSDQM 240

Query: 241 NMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGK 300
            M                            YV PV E+LDP   IRY+L+R ATKYENGK
Sbjct: 241 EM----------------------------YVLPVCEKLDPNGYIRYKLARGATKYENGK 300

Query: 301 HYRDLSKLNRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARN 360
           HYRDLSKLNRDP+KI+++S +AF+S+LQPENSVPIKP+K E DDTAL+D IPFLE+VARN
Sbjct: 301 HYRDLSKLNRDPKKILFVSANAFESTLQPENSVPIKPYKLEADDTALVDLIPFLEYVARN 360

Query: 361 SPADIRQVLESYKGCDIPTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           SPADIR VL S++  DI  EFI RS E+Q+R Q Q  QGR W+R
Sbjct: 361 SPADIRPVLASFERKDIAKEFIDRSIEYQKRKQGQLGQGRFWRR 376

BLAST of PI0003187 vs. ExPASy Swiss-Prot
Match: Q4I099 (Mitochondrial import inner membrane translocase subunit TIM50 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=TIM50 PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.1e-38
Identity = 90/231 (38.96%), Postives = 131/231 (56.71%), Query Frame = 0

Query: 148 EQVKGYTEPNAEMLLPDLHPMERHVFTLVLDLNETLVYSDWTRERGWQTFKRPGVDSFLE 207
           E V  Y EP  E LLPD  P     +TL L L++ L++S+WTRE GW+  KRPGVD F+ 
Sbjct: 213 ESVTYYQEPAFEKLLPDPDPTFERPYTLCLSLDDLLIHSEWTREHGWRIAKRPGVDYFIR 272

Query: 208 HLAQFYEIVIYSDQSNMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDPKHCI 267
           +L+Q+YE+V            LF  T  A                  +PV+ +LDP   I
Sbjct: 273 YLSQYYELV------------LFTTTPYAT----------------GEPVMRKLDPFRLI 332

Query: 268 RYRLSRAATKYENGKHYRDLSKLNRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDT 327
            + L R ATK+E+G+  +DLS LNRD  K+I +   A     QP+N++ + PWK + DD 
Sbjct: 333 LWPLYREATKFEDGEIVKDLSYLNRDLSKVIIIDTKAKHVRNQPDNAIILDPWKGDKDDK 392

Query: 328 ALLDFIPFLEFVARNSPADIRQVLESYKGCDIPTEFIRRSKEHQRRIQEQK 379
            L++ IPFLE++     +D+R+V++S+ G DIPTEF RR    ++  Q ++
Sbjct: 393 NLVNLIPFLEYIHTMQYSDVRKVIKSFDGKDIPTEFARREAIARKEFQAKQ 415

BLAST of PI0003187 vs. ExPASy Swiss-Prot
Match: Q5S7T7 (Mitochondrial import inner membrane translocase subunit TIM50 OS=Phytophthora infestans OX=4787 GN=TIM50 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.6e-38
Identity = 94/260 (36.15%), Postives = 143/260 (55.00%), Query Frame = 0

Query: 135 LVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMER------HVFTLVLDLNETLVYSDW 194
           L D+Y  + K +EE VK +T+P+ + LLPD  P+ +       V  LVLDL +TLV+S+W
Sbjct: 126 LGDIYAIIAKKVEETVKPFTDPSRQKLLPD-WPIPQVPADTPTVPVLVLDLEDTLVHSEW 185

Query: 195 TRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFNHYKVLIYFV 254
           +R+ GW+  KRPGVD FLE L Q+YEIVI+S                             
Sbjct: 186 SRKHGWRHAKRPGVDEFLETLCQYYEIVIFSQNYG------------------------- 245

Query: 255 LLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIYLSGHAFDSS 314
                 + ++++LDPK C  + LSR AT+Y NG H +DLS LNRD R+++ L        
Sbjct: 246 -----AEEIVQKLDPKQCALHILSRDATRYLNGAHVKDLSNLNRDLRQVVILDDDPAAYQ 305

Query: 315 LQPENSVPIKPWK--CETDDTALLDFIPFLEFVARNSPADIRQVLESYKG-----CDIPT 374
           LQPEN++P+ P+    + DD  L D IPFL+ +A     D RQV+  ++       D+ T
Sbjct: 306 LQPENAIPVTPFTNGRDRDDHELKDLIPFLKALASERVPDFRQVIGEFRDEDGVVRDLAT 354

Query: 375 EFIRRSKEHQRRIQEQKQQG 382
           ++  R  + + + +++K++G
Sbjct: 366 KYGARVHQLEMQKEQKKKKG 354

BLAST of PI0003187 vs. ExPASy Swiss-Prot
Match: Q4WI16 (Mitochondrial import inner membrane translocase subunit tim50 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=tim50 PE=3 SV=2)

HSP 1 Score: 159.8 bits (403), Expect = 6.0e-38
Identity = 84/244 (34.43%), Postives = 135/244 (55.33%), Query Frame = 0

Query: 139 YLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDLNETLVYSDWTRERGWQTFK 198
           Y  ++  + +    Y +P    LLPD  P  R  +TLVL L + LV+S+W+RE GW+  K
Sbjct: 189 YNRMKARLSDITSYYKDPAFPKLLPDEDPNLRQPYTLVLSLEDLLVHSEWSREHGWRIAK 248

Query: 199 RPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVI 258
           RPGVD FL +L Q+YE+V+++   +M+                             D V+
Sbjct: 249 RPGVDYFLRYLNQYYELVLFTSVPSMM----------------------------ADQVL 308

Query: 259 ERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIYLSGHAFDSSLQPENSVPIK 318
            +LDP   IR+ L R AT+Y++G++ +DLS LNRD  K+I +      + LQPEN++ + 
Sbjct: 309 RKLDPYRIIRWPLFREATRYKDGEYIKDLSYLNRDLSKVILIDTKEEHARLQPENAIILD 368

Query: 319 PWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDIPTEFIRRSKEHQRRIQEQK 378
            W  +  D  L+  IPFLE++A     D+R VL+S++G  IP EF +R +  + + +++ 
Sbjct: 369 KWLGDPKDKNLVALIPFLEYIAGMGVEDVRPVLKSFEGTSIPVEFAKRERIMREKFEKEL 404

Query: 379 QQGR 383
           ++ R
Sbjct: 429 EEER 404

BLAST of PI0003187 vs. ExPASy Swiss-Prot
Match: Q5B4P0 (Mitochondrial import inner membrane translocase subunit tim50 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=tim50 PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 7.8e-38
Identity = 88/235 (37.45%), Postives = 130/235 (55.32%), Query Frame = 0

Query: 153 YTEPNAEMLLPDLHPMERHVFTLVLDLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQF 212
           Y +P    LLPD  P  R  +TLVL L + LV+S+W+RE GW+  KRPGVD FL +L Q+
Sbjct: 232 YKDPAFPKLLPDEDPNLRQPYTLVLSLEDLLVHSEWSREHGWRVAKRPGVDYFLRYLNQY 291

Query: 213 YEIVIYSDQSNMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLS 272
           YE+V+++   +M+                             D V+ +LDP   IR+ L 
Sbjct: 292 YELVLFTSVPSMM----------------------------ADQVLRKLDPYRIIRWPLF 351

Query: 273 RAATKYENGKHYRDLSKLNRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDF 332
           R AT+Y++G++ +DLS LNRD  K+I +      + LQPEN++ +  W     D  L+  
Sbjct: 352 REATRYKDGEYIKDLSYLNRDLSKVILIDTKEEHARLQPENAIILDKWNGNPKDKTLVAL 411

Query: 333 IPFLEFVARNSPADIRQVLESYKGCDIPTEFIRRSKEHQRRI-----QEQKQQGR 383
           IPFLE++A     D+R VL+S++G  IP EF +R K  + R      +EQK++ R
Sbjct: 412 IPFLEYLAGMGVDDVRTVLKSFEGQSIPIEFAKREKAMRERFEKELAEEQKKRPR 438

BLAST of PI0003187 vs. ExPASy TrEMBL
Match: A0A1S3CIN1 (mitochondrial import inner membrane translocase subunit TIM50 OS=Cucumis melo OX=3656 GN=LOC103501237 PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 1.8e-194
Identity = 355/387 (91.73%), Postives = 357/387 (92.25%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSAT-SSS 60
           MSPL+FRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSAT SSS
Sbjct: 1   MSPLVFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSATSSSS 60

Query: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDK 120
           SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTA DDVSGVDK
Sbjct: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTAMDDVSGVDK 120

Query: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180
           VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL
Sbjct: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180

Query: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFN 240
           NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM               
Sbjct: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM--------------- 240

Query: 241 HYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300
                        YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY
Sbjct: 241 -------------YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300

Query: 301 LSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 360
           LSGHAF+SSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI
Sbjct: 301 LSGHAFESSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 359

Query: 361 PTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           PTEFIRRSKEHQRRIQEQKQQGRIWKR
Sbjct: 361 PTEFIRRSKEHQRRIQEQKQQGRIWKR 359

BLAST of PI0003187 vs. ExPASy TrEMBL
Match: A0A5D3C7P7 (Mitochondrial import inner membrane translocase subunit TIM50 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00490 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 8.1e-187
Identity = 341/373 (91.42%), Postives = 343/373 (91.96%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSAT-SSS 60
           MSPL+FRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSAT SSS
Sbjct: 1   MSPLVFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSATSSSS 60

Query: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDK 120
           SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTA DDVSGVDK
Sbjct: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTAMDDVSGVDK 120

Query: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180
           VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL
Sbjct: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180

Query: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFN 240
           NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM               
Sbjct: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM--------------- 240

Query: 241 HYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300
                        YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY
Sbjct: 241 -------------YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300

Query: 301 LSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 360
           LSGHAF+SSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI
Sbjct: 301 LSGHAFESSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 345

Query: 361 PTEFIRRSKEHQR 373
           PTEFIRRSKEHQR
Sbjct: 361 PTEFIRRSKEHQR 345

BLAST of PI0003187 vs. ExPASy TrEMBL
Match: A0A6J1IJ22 (mitochondrial import inner membrane translocase subunit TIM50 OS=Cucurbita maxima OX=3661 GN=LOC111476733 PE=4 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.6e-185
Identity = 335/389 (86.12%), Postives = 347/389 (89.20%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSATSSSS 60
           MSPLIFRSRF+SLLKNRHGRLMSSDLASTPSKDAINASQKL+SDQIPPD Q  + +SSSS
Sbjct: 1   MSPLIFRSRFVSLLKNRHGRLMSSDLASTPSKDAINASQKLISDQIPPDVQGPTGSSSSS 60

Query: 61  S---SKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGV 120
           S   SKPWNF KYSLIGALTGATAVAGYASYAY+YDE+EEKAKALRRSA+HTA DD SGV
Sbjct: 61  STTTSKPWNFLKYSLIGALTGATAVAGYASYAYTYDEVEEKAKALRRSANHTAADDASGV 120

Query: 121 DKVQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVL 180
           +KVQGMLYSTIM+ PAKL+DVYLDLRK IEEQVKGYTEPNAEMLLP+LHPMERHVFTLVL
Sbjct: 121 EKVQGMLYSTIMSAPAKLIDVYLDLRKAIEEQVKGYTEPNAEMLLPELHPMERHVFTLVL 180

Query: 181 DLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAA 240
           DLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM             
Sbjct: 181 DLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM------------- 240

Query: 241 FNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKI 300
                          YVDPVI+RLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKI
Sbjct: 241 ---------------YVDPVIDRLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKI 300

Query: 301 IYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGC 360
           IYLSGHA DSSLQPENSVPIKPWKCE DDTALLDFIPFLEFVARNSPADIRQVLESYKGC
Sbjct: 301 IYLSGHALDSSLQPENSVPIKPWKCEADDTALLDFIPFLEFVARNSPADIRQVLESYKGC 360

Query: 361 DIPTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           DIPTEFIRRSKEHQRRIQEQKQQGRIWKR
Sbjct: 361 DIPTEFIRRSKEHQRRIQEQKQQGRIWKR 361

BLAST of PI0003187 vs. ExPASy TrEMBL
Match: A0A6J1FGI0 (mitochondrial import inner membrane translocase subunit TIM50 OS=Cucurbita moschata OX=3662 GN=LOC111443698 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 7.1e-183
Identity = 332/388 (85.57%), Postives = 345/388 (88.92%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQ--TTSATSS 60
           MSPLI R RF+SLLKNRHGRLMSSDLASTPSKDAINASQKL+SDQIPPD Q  T S++SS
Sbjct: 1   MSPLILRPRFVSLLKNRHGRLMSSDLASTPSKDAINASQKLISDQIPPDVQGPTGSSSSS 60

Query: 61  SSSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVD 120
           S++SKPWNF KYSLIGALTGATAVAGYASYAY+ DEIEEKAKALRRSASHTA DD S V+
Sbjct: 61  STTSKPWNFLKYSLIGALTGATAVAGYASYAYTCDEIEEKAKALRRSASHTAADDASSVE 120

Query: 121 KVQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLD 180
           KVQGMLYSTIM+ PAKL+DVYLDLRK IE+QVKGYTEPNAEMLLP+LHPMERHVFTLVLD
Sbjct: 121 KVQGMLYSTIMSAPAKLIDVYLDLRKAIEKQVKGYTEPNAEMLLPELHPMERHVFTLVLD 180

Query: 181 LNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAF 240
           LNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM              
Sbjct: 181 LNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM-------------- 240

Query: 241 NHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKII 300
                         YVDPVI+RLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKII
Sbjct: 241 --------------YVDPVIDRLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKII 300

Query: 301 YLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCD 360
           YLSGHA DSSLQPENSVPIKPWKCE DDTALLDFIPFLEFVARNSPADIRQVLESYKGCD
Sbjct: 301 YLSGHALDSSLQPENSVPIKPWKCEADDTALLDFIPFLEFVARNSPADIRQVLESYKGCD 360

Query: 361 IPTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           IPTEFIRRSKEHQRRIQEQKQQGRIWKR
Sbjct: 361 IPTEFIRRSKEHQRRIQEQKQQGRIWKR 360

BLAST of PI0003187 vs. ExPASy TrEMBL
Match: A0A6J1FKX9 (mitochondrial import inner membrane translocase subunit TIM50-like OS=Cucurbita moschata OX=3662 GN=LOC111446192 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.9e-180
Identity = 333/390 (85.38%), Postives = 343/390 (87.95%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTT----SAT 60
           MSP IFRSRFISLLKNRHGRLMSSDLASTPSKD+I ASQKLVSDQIPPDAQ T    S++
Sbjct: 1   MSPPIFRSRFISLLKNRHGRLMSSDLASTPSKDSIIASQKLVSDQIPPDAQATSSSSSSS 60

Query: 61  SSSSSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSG 120
           SSSSSSKP NF KYSLI ALT  TAVAGYASYAYSYDEI EKAKALRRSAS+TA DDVSG
Sbjct: 61  SSSSSSKPLNFLKYSLISALTATTAVAGYASYAYSYDEIAEKAKALRRSASYTAMDDVSG 120

Query: 121 VDKVQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLV 180
           VDKVQG LYSTIMTVP KL DVYLDLRKTIEEQVKGYTEPNAEMLLPDLHP+E+HVFTLV
Sbjct: 121 VDKVQGRLYSTIMTVPVKLADVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPLEQHVFTLV 180

Query: 181 LDLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIA 240
           LDLNETLV+SDWTRERGWQTFKRPGVDSFLEHLAQFYEIVI+SDQSNM            
Sbjct: 181 LDLNETLVHSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIFSDQSNM------------ 240

Query: 241 AFNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRK 300
                           YVDPVI+RLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRK
Sbjct: 241 ----------------YVDPVIDRLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRK 300

Query: 301 IIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKG 360
           IIYLSGHAFDSSLQPENSVPIKPWKCE DDTALLDFIPFLEFVARNSPADIRQVLESY G
Sbjct: 301 IIYLSGHAFDSSLQPENSVPIKPWKCEADDTALLDFIPFLEFVARNSPADIRQVLESYNG 360

Query: 361 CDIPTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           CDIPTEFIRRSKEHQRRIQEQKQQGR+WKR
Sbjct: 361 CDIPTEFIRRSKEHQRRIQEQKQQGRLWKR 362

BLAST of PI0003187 vs. NCBI nr
Match: XP_008462988.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM50 [Cucumis melo])

HSP 1 Score: 688.3 bits (1775), Expect = 3.7e-194
Identity = 355/387 (91.73%), Postives = 357/387 (92.25%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSAT-SSS 60
           MSPL+FRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSAT SSS
Sbjct: 1   MSPLVFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSATSSSS 60

Query: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDK 120
           SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTA DDVSGVDK
Sbjct: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTAMDDVSGVDK 120

Query: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180
           VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL
Sbjct: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180

Query: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFN 240
           NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM               
Sbjct: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM--------------- 240

Query: 241 HYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300
                        YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY
Sbjct: 241 -------------YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300

Query: 301 LSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 360
           LSGHAF+SSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI
Sbjct: 301 LSGHAFESSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 359

Query: 361 PTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           PTEFIRRSKEHQRRIQEQKQQGRIWKR
Sbjct: 361 PTEFIRRSKEHQRRIQEQKQQGRIWKR 359

BLAST of PI0003187 vs. NCBI nr
Match: XP_031745212.1 (mitochondrial import inner membrane translocase subunit TIM50 [Cucumis sativus] >KAE8645967.1 hypothetical protein Csa_015462 [Cucumis sativus])

HSP 1 Score: 685.3 bits (1767), Expect = 3.1e-193
Identity = 353/387 (91.21%), Postives = 356/387 (91.99%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSA-TSSS 60
           MSPLIFRSRF SLLKNRHGRLMSSDLASTPSKDA+NASQKLVSDQIPPDAQTTSA +SSS
Sbjct: 1   MSPLIFRSRFFSLLKNRHGRLMSSDLASTPSKDALNASQKLVSDQIPPDAQTTSAISSSS 60

Query: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDK 120
           SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSG+DK
Sbjct: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGIDK 120

Query: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180
           VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL
Sbjct: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180

Query: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFN 240
           NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM               
Sbjct: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM--------------- 240

Query: 241 HYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300
                        YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY
Sbjct: 241 -------------YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300

Query: 301 LSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 360
           LSGHA DSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI
Sbjct: 301 LSGHASDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 359

Query: 361 PTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           PTEFIRRSKEHQRRIQEQKQQGRIWKR
Sbjct: 361 PTEFIRRSKEHQRRIQEQKQQGRIWKR 359

BLAST of PI0003187 vs. NCBI nr
Match: XP_038897595.1 (mitochondrial import inner membrane translocase subunit TIM50 [Benincasa hispida])

HSP 1 Score: 664.8 bits (1714), Expect = 4.4e-187
Identity = 345/387 (89.15%), Postives = 352/387 (90.96%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQ-TTSATSSS 60
           MSPL FRSRFISLLKNRHGRLMSS+LASTPSKDA+NASQKLVSDQIPPDAQ TT  T+SS
Sbjct: 1   MSPL-FRSRFISLLKNRHGRLMSSELASTPSKDALNASQKLVSDQIPPDAQPTTPTTTSS 60

Query: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDK 120
           SSSKP NF KYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDK
Sbjct: 61  SSSKPLNFLKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDK 120

Query: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180
           VQ MLYSTIMTVPAKLVDVYL+LRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL
Sbjct: 121 VQAMLYSTIMTVPAKLVDVYLNLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180

Query: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFN 240
           NETLVYSDWTRERGWQTFKRPGVD+FLEHLAQFYEIVIYSDQSNM               
Sbjct: 181 NETLVYSDWTRERGWQTFKRPGVDAFLEHLAQFYEIVIYSDQSNM--------------- 240

Query: 241 HYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300
                        YVDPVI+RLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKI+Y
Sbjct: 241 -------------YVDPVIDRLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKILY 300

Query: 301 LSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 360
           LSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI
Sbjct: 301 LSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 358

Query: 361 PTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           PTEFIRRSKEHQRRIQEQKQQGRIWKR
Sbjct: 361 PTEFIRRSKEHQRRIQEQKQQGRIWKR 358

BLAST of PI0003187 vs. NCBI nr
Match: TYK07933.1 (mitochondrial import inner membrane translocase subunit TIM50 [Cucumis melo var. makuwa])

HSP 1 Score: 662.9 bits (1709), Expect = 1.7e-186
Identity = 341/373 (91.42%), Postives = 343/373 (91.96%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSAT-SSS 60
           MSPL+FRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSAT SSS
Sbjct: 1   MSPLVFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQTTSATSSSS 60

Query: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGVDK 120
           SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTA DDVSGVDK
Sbjct: 61  SSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTAMDDVSGVDK 120

Query: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180
           VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL
Sbjct: 121 VQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVLDL 180

Query: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAAFN 240
           NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM               
Sbjct: 181 NETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM--------------- 240

Query: 241 HYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300
                        YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY
Sbjct: 241 -------------YVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKIIY 300

Query: 301 LSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 360
           LSGHAF+SSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI
Sbjct: 301 LSGHAFESSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGCDI 345

Query: 361 PTEFIRRSKEHQR 373
           PTEFIRRSKEHQR
Sbjct: 361 PTEFIRRSKEHQR 345

BLAST of PI0003187 vs. NCBI nr
Match: XP_023534832.1 (mitochondrial import inner membrane translocase subunit TIM50 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 660.6 bits (1703), Expect = 8.3e-186
Identity = 337/389 (86.63%), Postives = 349/389 (89.72%), Query Frame = 0

Query: 1   MSPLIFRSRFISLLKNRHGRLMSSDLASTPSKDAINASQKLVSDQIPPDAQ---TTSATS 60
           MSPLIFRSRF+SLLKNRHGRLMSSDLASTPSKDAINASQKL+SDQIPPD Q    +S++S
Sbjct: 1   MSPLIFRSRFVSLLKNRHGRLMSSDLASTPSKDAINASQKLISDQIPPDVQGPTGSSSSS 60

Query: 61  SSSSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTATDDVSGV 120
           SS++SKPWNF KYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTA DD SGV
Sbjct: 61  SSTTSKPWNFLKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRSASHTAADDASGV 120

Query: 121 DKVQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLLPDLHPMERHVFTLVL 180
           +KVQGMLYSTIM+ PAKL+DVYLDLRK IEEQVKGYTEPNAEMLLP+LHPMERHVFTLVL
Sbjct: 121 EKVQGMLYSTIMSAPAKLIDVYLDLRKAIEEQVKGYTEPNAEMLLPELHPMERHVFTLVL 180

Query: 181 DLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNMVICCLFILTLIAA 240
           DLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM             
Sbjct: 181 DLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQSNM------------- 240

Query: 241 FNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKI 300
                          YVDPVI+RLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKI
Sbjct: 241 ---------------YVDPVIDRLDPKHCIRYRLSRAATKYENGKHYRDLSKLNRDPRKI 300

Query: 301 IYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARNSPADIRQVLESYKGC 360
           IYLSGHA DSSLQPENSVPIKPWKCE DDTALLDFIPFLEFVARNSPADIRQVLESYKGC
Sbjct: 301 IYLSGHALDSSLQPENSVPIKPWKCEADDTALLDFIPFLEFVARNSPADIRQVLESYKGC 360

Query: 361 DIPTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           DIPTEFIRRSKEHQRRIQEQKQQGRIWKR
Sbjct: 361 DIPTEFIRRSKEHQRRIQEQKQQGRIWKR 361

BLAST of PI0003187 vs. TAIR 10
Match: AT1G55900.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 392.9 bits (1008), Expect = 3.0e-109
Identity = 214/404 (52.97%), Postives = 273/404 (67.57%), Query Frame = 0

Query: 1   MSPLIFRSRF---ISLLKNRHGRLMSSDLASTPSK---DAINASQKLVSD---------Q 60
           M+ ++ RSR    ++ L++R+ R  S++ +ST S      + ++Q + SD          
Sbjct: 1   MASIVLRSRLLPRLAKLRSRNLRCFSAEASSTNSTSRYSGVTSTQSMFSDFPPPNQPPPP 60

Query: 61  IPPDAQTTSATSSSSSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRS 120
            PP  +  +A ++    K   +  Y+L+ ALTGATA  GYAS+AY+ DE+ EK KA R S
Sbjct: 61  PPPQVEAAAAAATGKERKGLKYLGYALLWALTGATAATGYASFAYTIDEVNEKTKAFRES 120

Query: 121 ASHTATDDVSG---VDKVQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLL 180
           A+ T     SG   +DK Q  LYS  MT  A+ +D YL+LR+ +EEQVKG+TEP +E LL
Sbjct: 121 ATKTPVIKSSGIDVIDKYQTKLYSAAMTGSARAIDKYLELREIVEEQVKGFTEPLSEKLL 180

Query: 181 PDLHPMERHVFTLVLDLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQS 240
           PDLHP E+HVFTLVLDLNETL+Y+DW RERGW+TFKRPGVD+FLEHL +FYEIV+YSDQ 
Sbjct: 181 PDLHPAEQHVFTLVLDLNETLLYTDWKRERGWRTFKRPGVDAFLEHLGKFYEIVVYSDQM 240

Query: 241 NMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGK 300
            M                            YV PV E+LDP   IRY+L+R ATKYENGK
Sbjct: 241 EM----------------------------YVLPVCEKLDPNGYIRYKLARGATKYENGK 300

Query: 301 HYRDLSKLNRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARN 360
           HYRDLSKLNRDP+KI+++S +AF+S+LQPENSVPIKP+K E DDTAL+D IPFLE+VARN
Sbjct: 301 HYRDLSKLNRDPKKILFVSANAFESTLQPENSVPIKPYKLEADDTALVDLIPFLEYVARN 360

Query: 361 SPADIRQVLESYKGCDIPTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           SPADIR VL S++  DI  EFI RS E+Q+R Q Q  QGR W+R
Sbjct: 361 SPADIRPVLASFERKDIAKEFIDRSIEYQKRKQGQLGQGRFWRR 376

BLAST of PI0003187 vs. TAIR 10
Match: AT1G55900.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 4.0e-106
Identity = 211/404 (52.23%), Postives = 268/404 (66.34%), Query Frame = 0

Query: 1   MSPLIFRSRF---ISLLKNRHGRLMSSDLASTPSK---DAINASQKLVSD---------Q 60
           M+ ++ RSR    ++ L++R+ R  S++ +ST S      + ++Q + SD          
Sbjct: 1   MASIVLRSRLLPRLAKLRSRNLRCFSAEASSTNSTSRYSGVTSTQSMFSDFPPPNQPPPP 60

Query: 61  IPPDAQTTSATSSSSSSKPWNFFKYSLIGALTGATAVAGYASYAYSYDEIEEKAKALRRS 120
            PP  +  +A ++    K   +  Y+L+ ALTGATA  GYAS+AY+ DE+ EK KA R S
Sbjct: 61  PPPQVEAAAAAATGKERKGLKYLGYALLWALTGATAATGYASFAYTIDEVNEKTKAFRES 120

Query: 121 ASHTATDDVSG---VDKVQGMLYSTIMTVPAKLVDVYLDLRKTIEEQVKGYTEPNAEMLL 180
           A+ T     SG   +DK Q  LYS  MT  A+ +D YL+LR+ +EEQVKG+TEP +E LL
Sbjct: 121 ATKTPVIKSSGIDVIDKYQTKLYSAAMTGSARAIDKYLELREIVEEQVKGFTEPLSEKLL 180

Query: 181 PDLHPMERHVFTLVLDLNETLVYSDWTRERGWQTFKRPGVDSFLEHLAQFYEIVIYSDQS 240
           PDLHP E+HVFTLVLDLNETL+Y+DW RERGW+TFKRPGVD+FLEHL +FYEIV+YSDQ 
Sbjct: 181 PDLHPAEQHVFTLVLDLNETLLYTDWKRERGWRTFKRPGVDAFLEHLGKFYEIVVYSDQM 240

Query: 241 NMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAATKYENGK 300
            M                            YV PV E+LDP   IRY+L+R ATKYENGK
Sbjct: 241 EM----------------------------YVLPVCEKLDPNGYIRYKLARGATKYENGK 300

Query: 301 HYRDLSKLNRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFLEFVARN 360
           HYRDLSKLNRDP+KI+++S +AF+S+LQPENSVPIKP+K E DDTAL+D IPFLE+VARN
Sbjct: 301 HYRDLSKLNRDPKKILFVSANAFESTLQPENSVPIKPYKLEADDTALVDLIPFLEYVARN 360

Query: 361 SPADIRQVLESYKGCDIPTEFIRRSKEHQRRIQEQKQQGRIWKR 387
           SPADIR VL S++  DI  EFI       RR Q Q  QGR W+R
Sbjct: 361 SPADIRPVLASFERKDIAKEFI------DRRKQGQLGQGRFWRR 370

BLAST of PI0003187 vs. TAIR 10
Match: AT1G29780.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 4.4e-12
Identity = 55/182 (30.22%), Postives = 82/182 (45.05%), Query Frame = 0

Query: 174 TLVLDLNETLVYSDWTRERGWQ-----------------TFKRPGVDSFLEHLAQFYEIV 233
           T++LDL+ETLV++  T   G +                   KRPGV  FLE L +     
Sbjct: 51  TIILDLDETLVHAT-THLPGVKHDFMVMVKMEREIMPIFVVKRPGVTEFLERLGE----- 110

Query: 234 IYSDQSNMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRAAT 293
                                  +YKV+++   L +Y   V+++LD    I  RL R + 
Sbjct: 111 -----------------------NYKVVVFTAGLEEYASQVLDKLDKNGVISQRLYRDSC 170

Query: 294 KYENGKHYRDLS-KLNRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPF 338
              NGK+ +DLS  + +D R  + +  +    SLQPEN VPIK +  +  D  LL+ + F
Sbjct: 171 TEVNGKYVKDLSLVVGKDLRSALIVDDNPSSYSLQPENGVPIKAFVDDLKDQELLNLVEF 203

BLAST of PI0003187 vs. TAIR 10
Match: AT5G45700.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 4.9e-11
Identity = 50/180 (27.78%), Postives = 80/180 (44.44%), Query Frame = 0

Query: 174 TLVLDLNETLVYSDWTRERGWQTF----------------KRPGVDSFLEHLAQFYEIVI 233
           T+VLDL+ETLV+S   +      F                KRPGVD FL+ + + Y+IV+
Sbjct: 99  TIVLDLDETLVHSSMEKPEVPYDFVVNPKIDGQILTFFVIKRPGVDEFLKKIGEKYQIVV 158

Query: 234 YSDQSNMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDP-KHCIRYRLSRAAT 293
           ++                              L +Y   V+++LDP +  I     R A 
Sbjct: 159 FT----------------------------AGLREYASLVLDKLDPERRVISRSFYRDAC 218

Query: 294 KYENGKHYRDLSKLNRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFIPFL 337
              +G+  +DL  + RD R+++ +  +    +LQPEN+ PIKP+  + +D  L     FL
Sbjct: 219 SEIDGRLVKDLGFVMRDLRRVVIVDDNPNSYALQPENAFPIKPFSDDLEDVELKKLGEFL 250

BLAST of PI0003187 vs. TAIR 10
Match: AT1G29770.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 2.4e-10
Identity = 51/184 (27.72%), Postives = 80/184 (43.48%), Query Frame = 0

Query: 174 TLVLDLNETLVYSDWTRERGWQ-------------------TFKRPGVDSFLEHLAQFYE 233
           T+ LDL+ETLV+S  T E   +                     KRPGV  FLE +++   
Sbjct: 104 TIFLDLDETLVHS--TMEPPIRVNVDFMVRIKIEGAVIPMFVVKRPGVTEFLERISK--- 163

Query: 234 IVIYSDQSNMVICCLFILTLIAAFNHYKVLIYFVLLLQYVDPVIERLDPKHCIRYRLSRA 293
                                    +Y+V I+   L +Y   V+++LD    I  RL R 
Sbjct: 164 -------------------------NYRVAIFTAGLPEYASQVLDKLDKNRVISQRLYRD 223

Query: 294 ATKYENGKHYRDLSKL-NRDPRKIIYLSGHAFDSSLQPENSVPIKPWKCETDDTALLDFI 338
           +    NG++ +DLS +   D   ++ +  + F  SLQP+N VPIKP+  + +D  L+   
Sbjct: 224 SCTEVNGRYAKDLSLVAKNDLGSVLLVDDNPFSYSLQPDNGVPIKPFMDDMEDQELMKLA 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYE24.2e-10852.97Mitochondrial import inner membrane translocase subunit TIM50 OS=Arabidopsis tha... [more]
Q4I0992.1e-3838.96Mitochondrial import inner membrane translocase subunit TIM50 OS=Gibberella zeae... [more]
Q5S7T74.6e-3836.15Mitochondrial import inner membrane translocase subunit TIM50 OS=Phytophthora in... [more]
Q4WI166.0e-3834.43Mitochondrial import inner membrane translocase subunit tim50 OS=Neosartorya fum... [more]
Q5B4P07.8e-3837.45Mitochondrial import inner membrane translocase subunit tim50 OS=Emericella nidu... [more]
Match NameE-valueIdentityDescription
A0A1S3CIN11.8e-19491.73mitochondrial import inner membrane translocase subunit TIM50 OS=Cucumis melo OX... [more]
A0A5D3C7P78.1e-18791.42Mitochondrial import inner membrane translocase subunit TIM50 OS=Cucumis melo va... [more]
A0A6J1IJ222.6e-18586.12mitochondrial import inner membrane translocase subunit TIM50 OS=Cucurbita maxim... [more]
A0A6J1FGI07.1e-18385.57mitochondrial import inner membrane translocase subunit TIM50 OS=Cucurbita mosch... [more]
A0A6J1FKX91.9e-18085.38mitochondrial import inner membrane translocase subunit TIM50-like OS=Cucurbita ... [more]
Match NameE-valueIdentityDescription
XP_008462988.13.7e-19491.73PREDICTED: mitochondrial import inner membrane translocase subunit TIM50 [Cucumi... [more]
XP_031745212.13.1e-19391.21mitochondrial import inner membrane translocase subunit TIM50 [Cucumis sativus] ... [more]
XP_038897595.14.4e-18789.15mitochondrial import inner membrane translocase subunit TIM50 [Benincasa hispida... [more]
TYK07933.11.7e-18691.42mitochondrial import inner membrane translocase subunit TIM50 [Cucumis melo var.... [more]
XP_023534832.18.3e-18686.63mitochondrial import inner membrane translocase subunit TIM50 [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
AT1G55900.13.0e-10952.97Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT1G55900.24.0e-10652.23Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT1G29780.14.4e-1230.22Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G45700.14.9e-1127.78Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT1G29770.12.4e-1027.72Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004274FCP1 homology domainSMARTSM00577forpap2coord: 171..327
e-value: 2.9E-36
score: 136.5
IPR004274FCP1 homology domainPFAMPF03031NIFcoord: 174..224
e-value: 4.3E-13
score: 49.3
coord: 237..348
e-value: 1.6E-19
score: 70.2
IPR004274FCP1 homology domainPROSITEPS50969FCP1coord: 168..339
score: 26.737339
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 158..369
e-value: 8.0E-45
score: 154.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..62
NoneNo IPR availablePANTHERPTHR12210:SF153MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM50-LIKEcoord: 5..225
NoneNo IPR availablePANTHERPTHR12210:SF153MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM50-LIKEcoord: 240..341
NoneNo IPR availablePANTHERPTHR12210NUCLEAR LIM INTERACTOR-INTERACTING FACTOR-RELATEDcoord: 5..225
coord: 240..341
NoneNo IPR availableCDDcd07521HAD_FCP1-likecoord: 173..317
e-value: 2.98254E-37
score: 129.638
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 234..351
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 160..224

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003187.2PI0003187.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0005744 TIM23 mitochondrial import inner membrane translocase complex