PI0003181 (gene) Melon (PI 482460) v1

Overview
NamePI0003181
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionLeucine aminopeptidase 1-like
Locationchr05: 4373057 .. 4377444 (+)
RNA-Seq ExpressionPI0003181
SyntenyPI0003181
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTTCATGCCCAATATAGTTCCTCAAACTATTGGTCTTAATTATCCTGGTTCGAGCAATGCCCCTAAGGTTTGTAAGACATACCAAATTTAATGTTAATCTATTTATTGTTGGTTTTTCTTCCATTGTCATTTTTCGAAGCCCTTTCGTTTATTTAACTTTTCTTAAATTTGAAAGGTTTAATGTGCATCGTTGGTTTTGATTCACGACTTTTGAATGTTTAGTTTTTAGTGGTGAAGAACTTTAAGTATATCAATAAGATAGATCAATAAAAGTCTCTATTGGTATGAAATGTTTTTGGTAAAAAGGTAAAAAGGTAAAAAGTTAAAAAGTAAATCTAGTGGAGTTGAAAAGGACAATATTTTATACAAGAAAAGTCAATTTATACCTCTAAACTTTAAGAGTTGTATCAATTAAAGTCCTGAATTAATCATTGTATCAATTCAAACCATGAACTTTACTGAGTATATCAATTTATACTCTTATTTATATATATATATATGTAAGGAAAAATTTTGGAAAGTACTAATAGAGTATGTTACCTGTTTATTAAAATGTCTCAAAGGCAACTTTTGCTGCAAAAGACATTGATGCCTTGGAATGGAAGGGAGACTTGCTTGCTGTGGGTGTGACAGAGAAAGATGTGGTGAAGGATGAAAACTCCAATTTCAAGAATCCACTTTTGCACAAGTTGGATGCAATTTTGGTGGATTGTTGGATGAAGCTTTTTCAAAGGAATATTTCTCTGGGAAGTCAGGCCAATCTGTTGTTCTAACAGTTTCTGGTTTGTGTTTTAAGAGGGTTGGTTTGTTTGGGCTTGGCCAGTCAGCTTCTAGTGCAACAGACTTTGATGGTCTAGGTGAGACCATTGCAGCAGCAGGAAAGACATCTGGAGCAGCTTCCGTTGCTGTAGCTCTTGCCTTCTCAGAGGACCTTTCCGATGAATCAAAGCCTGAAATTGCTTCCATCATTGCAGTTGGTACATGTTCTATAGTCTTGAAACTATGTACTTACTCGAAATTACTATTAAAAAAGTAGCTGCAAACACACCTTAGTTCTTGAGTGTATAGTTTGTTGTCCTATTTGACACTAATTTTTTTCTGCATCATAGGGATTGCCAATGGGATATTTGACGACGATATTACTCTACTTCGATCCGTGGATATTCTTGGTCTTGGATCTGGACCTGATATGGACAAAAAGCTGAAATATGCACAAAGCGTCAGTTCAGGGATAATGTATGTAGAAGAACTTGAAAATTCACCTGCAAATGTGCTCACAACAGGTTCGCTTCTTTATGTTTTATTTCAATATGTCATTTACTTTTTAAGTTTTTGCAGTTTTGCATATCAGTGTATGTACCCTTGAACTAATATTGAATTTCAACGTAATGTAGCATGCCATAGTATTTATATGTATCCTTGAAGTAATACTCAGTTCAACTTGATGTAGCATGAAATAGCTTGTAACCATACAGGGTACTAAGTTTGCTAAATTATATCCACTTGATTAGAGACATTGACAGCAGAGGTTTTAAAGTTGACCCAAATGTACAACGATGTTCTTTCTGCAAGAATTTTTGATGGAGAAGAAATCATAGAAGTGAAGATGGGATCCTTTCTTGGTGACACTGCAAATCCACCCAAGTTTATCCACTTATGTTACAAACCTCCCAGTGGACCTGTGTCAACCAAATTGGGTTTAGCTGGTAAAGGAATTACCTTTGACAGGTGATCATACATCTAACAGCATTTTCACTCAAGATTATCATGTTTGAGAGTGACATAAAAGTATCTTTGACCCAAAATCTATTGAACTAATTTCTTAGAAGCAATCTAGAATTTTGACCCAAAAGCTTGACAGAGAAGCACTTCAAAAGTATCTTTAAGAGTGTAATGCCAAACATCAAAGTGGTTTGTTCCTCAACTAAGTTTTAAGAAAATACTTAAAGCACTATTGCTAAAAATGGCCTTAACCACACCTTAAACCAACATAAGATTTTTGTTGTGATGATATTTATGCAATAGCATATTAACGTTCCATGTCATTTCATGTTAATGATATTATATATCTTGTAAGATGTGGTTTATTCTACTTTGAACACAAATGGCAGAGGGTGTGTTTGGGAGTGATAGTTGAGGTTTGGATTTTAGGAAAAGAGATTTAGGTACAATTGATTTTTAAACAACCAAATTTATGTTTGGTACCAAACTCTTACAAGTAATTTTAATATATTTAGAATTAATTTTAAATCATTATGTTTGGTTCTAAAAGTTAATTTAGAGTTACCCAATTAGTCATTGTATTTTCGATTTGAGAAATAATTATGGTAAAGATCTATTTCTATTTATTGTTAAATCTTATATTATAAATATTGAAAGAAAACTCAAAACATATATAATTGTTTGTTTTATTAACAATTTAATGACATGCAAGTGTTTGTTTTATTAACAATTTAATGACATATTTTGGCAAGAGAAAAAATGATTTTGACCATCTCAAAATCACTCCCAAACACGCCCTAACTAATTCTCTTATTTCTCCTTTTCAATAGTGGTGGCTTCAACCTTAAAACAGGAGGCTACAGTAAAATTGAACTACTGAAAAATGACATGGGAGGGGCATCAACTATTTTAGGTGCAGCCAAATCCATTGCTGAAATTAAACCTCTTGGTGTAGAGGTAAAACATTAGATATATCACTCCTTTCCATGTGATTATGTTCTTGATTGATCAATAGGTCTAACTGGGGCTTGATTTTCTTGATCCCAAACAGGTTCATTTTGTGGTTCCTGCCTCTAAGAACATGATAAGTGCAACTGGCTCGACACTGAATGATATTGCTACAACTTCAAATGAAAATAAAAATGAGGTATAGCGTATATTCTTAGGTTCATTGACCCAGAAAAAGGATCATTGGGCTATCATCATTCTGATAATTTTTGTTTGTGTTAAATTTGGCTCTACAATATTTGTTATTTTTATCTCAGGGGACAACCATCTTGACCCTACAATATTTGGGTGTAAAAATAAAAATCGTCATTCCTAGGTAGGTGGTTACCATGTATTGAACACGTGACTTCTTAGTTAGTTATTGAAACTATGTCTCCTTTTTTACCACTAGGTCAACCCACGATAGTCAATAAAATAGTGATACCAGTGAGTAATTTTGGGAGTTGTAAACTACGTGGAAGTATATTGTTCTCTCTCCTTTCCTCTTTCGTTACAATCATATTAAGGTACCTATTTAAATTATTAAGTATTAATTATCTTTCATTTTGGATAATTTTCAGGTTAATAATAAGGGCAGATTTAGCCTTGCTTTGATACACGCTTCTAAGCAGGACGTTGATAAGGTAAATATATAATAAGATAGTATTAAAAAATTTATTGACCCAGATACATAAATGCTAATAGTTTTGGGAGTTGGATATAATGCAACACCAAATTGCTTTGCAGAATATTGAACTGCCTACTCTAACTGATGCTTGGACAACTGCTCGTGGACCGGCAGTTGGAGGTATGATAATCTTCATAGCATCTAATGCATCATAGTCAATTTGAAGATTATAACATAAAATTAGATTTTATGCGATTTGTAAAGAGAAAGGACATTAAATAAACTTGTTTTCCATAAAAAAAAAAAAAAAAACCAATTGAAAATAGAGGAGTAACCAACATATCTTATAAAGATTATTAGATCTTTTTATTTTTGCAACGTGGGATCTTCAACAAATAACAAAAAAAGGAGTTCCAAATGCAATATGGAGCGATCAAAATGATTAACATTAGAGACACTTCGATTGGATTGTGATAAAAGGAGAGTTTTTTTTTCTTCTCCCTTTCTTGGAATGGTGGGTGGGTTACTATTTAAAGTTAAGCCTTCTATGGATCAACAATGAAAGAATGTGATTGTTACAAGACTTCTTTTTGTTTTTCAGGTGTCTTTAAGCCAACTGAACACCTAGGAAAGAAGGTAACTTCTGCTGCAGAGAGGAGTGGTGAAAAACTATGGAACGCTGGTTCCGCTCAAGGAGGTTCTATAACAGCTGATATGTTTCTAAAACAGGTGGATCTTATCTTCTTATTTCTTGAACTGCTTGAAAGGGAGAAGTTTCATCTCCATCTCTTTTGTTCCTTCATTCCTCATTTATGTTTATACCAACCAAAGGATAACTTAAAGAGATTGTCCTTTGAAAGTTTTATGCCTTGAATTTCTAAAACTTGAAAATCTATTTACGTAGATTGTTGATGAGAATGTCCAATCGATGCACCATGACGTTGCTGGCCCTTTCTGGAATACCGAGAAGACTACTGTTGTAACTGGACTTGGTGTTTCCACATTAGTGGATTGGGTTTTGAAGAATGCTTCTTCTTAAAAGTCCA

mRNA sequence

ATGAGCTTCATGCCCAATATAGTTCCTCAAACTATTGGTCTTAATTATCCTGGTTCGAGCAATGCCCCTAAGGCAACTTTTGCTGCAAAAGACATTGATGCCTTGGAATGGAAGGGAGACTTGCTTGCTGTGGGTGTGACAGAGAAAGATGTGGTGAAGGATGAAAACTCCAATTTCAAGAATCCACTTTTGCACAAGTTGGATGCAATTTTGAGGGTTGGTTTGTTTGGGCTTGGCCAGTCAGCTTCTAGTGCAACAGACTTTGATGGTCTAGGTGAGACCATTGCAGCAGCAGGAAAGACATCTGGAGCAGCTTCCGTTGCTGTAGCTCTTGCCTTCTCAGAGGACCTTTCCGATGAATCAAAGCCTGAAATTGCTTCCATCATTGCAGTTGGGATTGCCAATGGGATATTTGACGACGATATTACTCTACTTCGATCCGTGGATATTCTTGGTCTTGGATCTGGACCTGATATGGACAAAAAGCTGAAATATGCACAAAGCGTCAGTTCAGGGATAATGTATGTAGAAGAACTTGAAAATTCACCTGCAAATGTGCTCACAACAGAGACATTGACAGCAGAGGTTTTAAAGTTGACCCAAATGTACAACGATGTTCTTTCTGCAAGAATTTTTGATGGAGAAGAAATCATAGAAGTGAAGATGGGATCCTTTCTTGGTGACACTGCAAATCCACCCAAGTTTATCCACTTATGTTACAAACCTCCCAGTGGACCTGTGTCAACCAAATTGGGTTTAGCTGGTAAAGGAATTACCTTTGACAGTGGTGGCTTCAACCTTAAAACAGGAGGCTACAGTAAAATTGAACTACTGAAAAATGACATGGGAGGGGCATCAACTATTTTAGGTGCAGCCAAATCCATTGCTGAAATTAAACCTCTTGGTGTAGAGGTTCATTTTGTGGTTCCTGCCTCTAAGAACATGATAAGTGCAACTGGCTCGACACTGAATGATATTGCTACAACTTCAAATGAAAATAAAAATGAGGTTAATAATAAGGGCAGATTTAGCCTTGCTTTGATACACGCTTCTAAGCAGGACGTTGATAAGAATATTGAACTGCCTACTCTAACTGATGCTTGGACAACTGCTCGTGGACCGGCAGTTGGAGGTGTCTTTAAGCCAACTGAACACCTAGGAAAGAAGGTAACTTCTGCTGCAGAGAGGAGTGGTGAAAAACTATGGAACGCTGGTTCCGCTCAAGGAGGTTCTATAACAGCTGATATGTTTCTAAAACAGATTGTTGATGAGAATGTCCAATCGATGCACCATGACGTTGCTGGCCCTTTCTGGAATACCGAGAAGACTACTGTTGTAACTGGACTTGGTGTTTCCACATTAGTGGATTGGGTTTTGAAGAATGCTTCTTCTTAAAAGTCCA

Coding sequence (CDS)

ATGAGCTTCATGCCCAATATAGTTCCTCAAACTATTGGTCTTAATTATCCTGGTTCGAGCAATGCCCCTAAGGCAACTTTTGCTGCAAAAGACATTGATGCCTTGGAATGGAAGGGAGACTTGCTTGCTGTGGGTGTGACAGAGAAAGATGTGGTGAAGGATGAAAACTCCAATTTCAAGAATCCACTTTTGCACAAGTTGGATGCAATTTTGAGGGTTGGTTTGTTTGGGCTTGGCCAGTCAGCTTCTAGTGCAACAGACTTTGATGGTCTAGGTGAGACCATTGCAGCAGCAGGAAAGACATCTGGAGCAGCTTCCGTTGCTGTAGCTCTTGCCTTCTCAGAGGACCTTTCCGATGAATCAAAGCCTGAAATTGCTTCCATCATTGCAGTTGGGATTGCCAATGGGATATTTGACGACGATATTACTCTACTTCGATCCGTGGATATTCTTGGTCTTGGATCTGGACCTGATATGGACAAAAAGCTGAAATATGCACAAAGCGTCAGTTCAGGGATAATGTATGTAGAAGAACTTGAAAATTCACCTGCAAATGTGCTCACAACAGAGACATTGACAGCAGAGGTTTTAAAGTTGACCCAAATGTACAACGATGTTCTTTCTGCAAGAATTTTTGATGGAGAAGAAATCATAGAAGTGAAGATGGGATCCTTTCTTGGTGACACTGCAAATCCACCCAAGTTTATCCACTTATGTTACAAACCTCCCAGTGGACCTGTGTCAACCAAATTGGGTTTAGCTGGTAAAGGAATTACCTTTGACAGTGGTGGCTTCAACCTTAAAACAGGAGGCTACAGTAAAATTGAACTACTGAAAAATGACATGGGAGGGGCATCAACTATTTTAGGTGCAGCCAAATCCATTGCTGAAATTAAACCTCTTGGTGTAGAGGTTCATTTTGTGGTTCCTGCCTCTAAGAACATGATAAGTGCAACTGGCTCGACACTGAATGATATTGCTACAACTTCAAATGAAAATAAAAATGAGGTTAATAATAAGGGCAGATTTAGCCTTGCTTTGATACACGCTTCTAAGCAGGACGTTGATAAGAATATTGAACTGCCTACTCTAACTGATGCTTGGACAACTGCTCGTGGACCGGCAGTTGGAGGTGTCTTTAAGCCAACTGAACACCTAGGAAAGAAGGTAACTTCTGCTGCAGAGAGGAGTGGTGAAAAACTATGGAACGCTGGTTCCGCTCAAGGAGGTTCTATAACAGCTGATATGTTTCTAAAACAGATTGTTGATGAGAATGTCCAATCGATGCACCATGACGTTGCTGGCCCTTTCTGGAATACCGAGAAGACTACTGTTGTAACTGGACTTGGTGTTTCCACATTAGTGGATTGGGTTTTGAAGAATGCTTCTTCTTAA

Protein sequence

MSFMPNIVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKLDAILRVGLFGLGQSASSATDFDGLGETIAAAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDDITLLRSVDILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARIFDGEEIIEVKMGSFLGDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIATTSNENKNEVNNKGRFSLALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKPTEHLGKKVTSAAERSGEKLWNAGSAQGGSITADMFLKQIVDENVQSMHHDVAGPFWNTEKTTVVTGLGVSTLVDWVLKNASS
Homology
BLAST of PI0003181 vs. ExPASy Swiss-Prot
Match: Q944P7 (Leucine aminopeptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LAP2 PE=2 SV=2)

HSP 1 Score: 447.6 bits (1150), Expect = 1.7e-124
Identity = 261/520 (50.19%), Postives = 332/520 (63.85%), Query Frame = 0

Query: 11  TIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKLDAI 70
           T+GL    S + PK +F+ K+ID  EWKGD+LAVGVTEKD+ KD NS F+NP+L KLDA 
Sbjct: 65  TLGLTQANSVDHPKISFSGKEIDVTEWKGDILAVGVTEKDMAKDVNSKFENPILKKLDAH 124

Query: 71  L-------------------------------RVGLFGLGQSASSATDFDGLGETIAAAG 130
           L                               RVGL GLG+SAS+ + F  LGE +AAA 
Sbjct: 125 LGGLLADVSSEEDFSGKPGQSTVLRLPGLGSKRVGLIGLGKSASTPSAFQSLGEAVAAAA 184

Query: 131 KTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVDILG 190
           K S A+SVAV LA SE +S+ESK   AS IA G   G+F+D           L+SVDI+G
Sbjct: 185 KASQASSVAVVLASSESVSNESKLCSASAIASGTVLGLFEDSRYKSESKKPSLKSVDIIG 244

Query: 191 LGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARIF 250
            GSGP+++KKLKYA+ VS G+++ +EL NSPANVLT   L  E L L  MY+DV++A I 
Sbjct: 245 FGSGPELEKKLKYAEHVSYGVIFGKELVNSPANVLTPAVLAEEALNLASMYSDVMTANIL 304

Query: 251 DGEEIIEVKMGSFL---GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNLKT 310
           + E+  E+KMGS+L     +ANPP FIHL YKP SGPV TKL L GKG+TFDSGG+N+KT
Sbjct: 305 NEEQCKELKMGSYLAVAAASANPPHFIHLIYKPSSGPVKTKLALVGKGLTFDSGGYNIKT 364

Query: 311 GGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIATT 370
           G    IEL+K DMGG++ +LGAAK+I +IKP GVEVHF+V A +NMIS TG    D+ T 
Sbjct: 365 GPGCLIELMKFDMGGSAAVLGAAKAIGQIKPPGVEVHFIVAACENMISGTGMRPGDVLTA 424

Query: 371 SNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKPTE 430
           SN    EVNN   +GR +L  AL++A  Q VDK ++L TLT A   A G ++ G++ P++
Sbjct: 425 SNGKTIEVNNTDAEGRLTLADALVYACNQGVDKVVDLATLTGACIIALGTSMAGIYTPSD 484

Query: 431 HLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQIVDE 465
            L K+V +A+ERSGEKLW                    N G   GGSITA +FLKQ V E
Sbjct: 485 KLAKEVIAASERSGEKLWRMPMEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSE 544

BLAST of PI0003181 vs. ExPASy Swiss-Prot
Match: P31427 (Leucine aminopeptidase, chloroplastic OS=Solanum tuberosum OX=4113 GN=LAP PE=2 SV=2)

HSP 1 Score: 436.8 bits (1122), Expect = 3.1e-121
Identity = 250/520 (48.08%), Postives = 324/520 (62.31%), Query Frame = 0

Query: 7   IVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHK 66
           I   T+GL  P  S+APK +  AKD D ++W+GDLLA+G TE D+ +D+NS FKNPLL +
Sbjct: 54  IAGDTLGLTRPNESDAPKISIGAKDTDVVQWQGDLLAIGATENDLARDDNSKFKNPLLQR 113

Query: 67  LDAIL----------------------------RVGLFGLGQSASSATDFDGLGETIAAA 126
           LD+ L                            R+ L GLG SASS T +  LGE  AAA
Sbjct: 114 LDSKLNGLLSAASSEEDFSGKSGQSINLRLPGGRITLVGLGSSASSPTSYHSLGEAAAAA 173

Query: 127 GKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVDIL 186
            K++ A ++AV+LA ++ LS ESK   AS IA G+  GIF+D+          L S+DIL
Sbjct: 174 AKSAQARNIAVSLASTDGLSAESKINSASAIATGVMLGIFEDNRFRSESKTPALESLDIL 233

Query: 187 GLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARI 246
           GLG+GP+++ K+KYA+ V +G++   EL N+PAN++T   L  E  K+   Y+DV++  I
Sbjct: 234 GLGTGPEIESKIKYAEHVCAGVILGRELVNAPANIVTPGALAEEAKKIASTYSDVITVNI 293

Query: 247 FDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNL 306
            D E+  E+KMG++LG     T NPP FIHLC+K  S    TK+ L GKG+TFDSGG+NL
Sbjct: 294 LDAEQCKELKMGAYLGVAAAATENPPYFIHLCFKTNSRERKTKIALVGKGLTFDSGGYNL 353

Query: 307 KTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIA 366
           KTG  SKIEL+KNDMGGA+ +LGAAK++ EIKP GVEVHF+V A +NMIS  G    DI 
Sbjct: 354 KTGAGSKIELMKNDMGGAAAVLGAAKALGEIKPRGVEVHFIVAACENMISGAGMRPGDIV 413

Query: 367 TTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKP 426
           T SN    EVNN   +GR +L  ALI+A  Q V+K I+L TLT A  TA GP+V G F P
Sbjct: 414 TASNGKTIEVNNTDAEGRLTLADALIYACNQGVEKIIDLATLTGAIVTALGPSVAGAFTP 473

Query: 427 TEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQIV 463
           ++ L ++V  AAE SGEKLW                    N G   GG+IT  +FLKQ V
Sbjct: 474 SDGLAREVVVAAEASGEKLWRMPMEESYWESMKSGVADMINTGPRDGGAITGALFLKQFV 533

BLAST of PI0003181 vs. ExPASy Swiss-Prot
Match: Q10712 (Leucine aminopeptidase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=LAPA1 PE=1 SV=2)

HSP 1 Score: 429.5 bits (1103), Expect = 4.9e-119
Identity = 248/519 (47.78%), Postives = 320/519 (61.66%), Query Frame = 0

Query: 7   IVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHK 66
           I   T+GL  P  S+APK +  AKD   ++W+GDLLA+G TE D+ +DENS FKNPLL +
Sbjct: 54  IAGDTLGLTRPNESDAPKISIGAKDTAVVQWQGDLLAIGATENDMARDENSKFKNPLLQQ 113

Query: 67  LDAIL----------------------------RVGLFGLGQSASSATDFDGLGETIAAA 126
           LD+ L                            R+ L GLG SASS T +  LG+  AAA
Sbjct: 114 LDSELNGLLSAASSEEDFSGKSGQSVNLRFPGGRITLVGLGSSASSPTSYHSLGQAAAAA 173

Query: 127 GKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVDIL 186
            K+S A ++AVALA ++ LS ESK   AS IA G+  G F+D+        + L S+DIL
Sbjct: 174 AKSSQARNIAVALASTDGLSAESKINSASAIATGVVLGSFEDNRFRSESKKSTLESLDIL 233

Query: 187 GLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARI 246
           GLG+GP++++K+KYA+ V +G++   EL N+PAN++T   L  E  K+   Y+DV+S  I
Sbjct: 234 GLGTGPEIERKIKYAEHVCAGVILGRELVNAPANIVTPAVLAEEAKKIASTYSDVISVNI 293

Query: 247 FDGEEIIEVKMGSFL----GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNL 306
            D E+  E+KMG++L      T NPP FIHLC+K P+    TKL L GKG+TFDSGG+NL
Sbjct: 294 LDAEQCKELKMGAYLAVAAAATENPPYFIHLCFKTPTKERKTKLALVGKGLTFDSGGYNL 353

Query: 307 KTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIA 366
           K G  S+IEL+KNDMGGA+ +LGAAK++ EI+P  VEVHF+V A +NMISA G    DI 
Sbjct: 354 KVGAGSRIELMKNDMGGAAAVLGAAKALGEIRPSRVEVHFIVAACENMISAEGMRPGDIV 413

Query: 367 TTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKP 426
           T SN    EVNN   +GR +L  ALI+A  Q V+K I+L TLT A   A GP+V G F P
Sbjct: 414 TASNGKTIEVNNTDAEGRLTLADALIYACNQGVEKIIDLATLTGAIMVALGPSVAGAFTP 473

Query: 427 TEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQIV 462
            + L ++V  AAE SGEKLW                    N G   GG+IT  +FLKQ V
Sbjct: 474 NDDLAREVVEAAEASGEKLWRMPMEESYWESMKSGSGDMINTGPGNGGAITGALFLKQFV 533

BLAST of PI0003181 vs. ExPASy Swiss-Prot
Match: P30184 (Leucine aminopeptidase 1 OS=Arabidopsis thaliana OX=3702 GN=LAP1 PE=1 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 8.3e-119
Identity = 251/520 (48.27%), Postives = 320/520 (61.54%), Query Frame = 0

Query: 11  TIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKLDAI 70
           T+GL  P S+   K +F AK+ID +EWKGD+L VGVTEKD+ KD NS F+NP+L K+DA 
Sbjct: 4   TLGLTQPNSTEPHKISFTAKEIDVIEWKGDILVVGVTEKDLAKDGNSKFENPILSKVDAH 63

Query: 71  L-------------------------------RVGLFGLGQSASSATDFDGLGETIAAAG 130
           L                               R+ L GLGQS SS   F  LGE +A   
Sbjct: 64  LSGLLAQVSSEEDFTGKPGQSTVLRLPGLGSKRIALIGLGQSVSSPVAFHSLGEAVATVS 123

Query: 131 KTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDD-------DITLLRSVDILG 190
           K S + S A+ LA S  +SDESK    S +A GI  G+F+D           L++VDI+G
Sbjct: 124 KASQSTSAAIVLASS--VSDESKLSSVSALASGIVLGLFEDGRYKSESKKPSLKAVDIIG 183

Query: 191 LGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARIF 250
            G+G +++KKLKYA+ VS G+++  EL NSPANVLT   L  E  K+   Y+DV +A I 
Sbjct: 184 FGTGAEVEKKLKYAEDVSYGVIFGRELINSPANVLTPAVLAEEAAKVASTYSDVFTANIL 243

Query: 251 DGEEIIEVKMGSFL---GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNLKT 310
           + E+  E+KMGS+L     +ANPP FIHL YKPP+G V TKL L GKG+TFDSGG+N+KT
Sbjct: 244 NEEQCKELKMGSYLAVAAASANPPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKT 303

Query: 311 GGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIATT 370
           G    IEL+K DMGG++ +LGAAK+I EIKP GVEVHF+V A +NMIS TG    D+ T 
Sbjct: 304 GPGCSIELMKFDMGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITA 363

Query: 371 SNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKPTE 430
           SN    EVNN   +GR +L  AL++A  Q VDK ++L TLT A   A G ++ G++ P++
Sbjct: 364 SNGKTIEVNNTDAEGRLTLADALVYACNQGVDKIVDLATLTGACVIALGTSMAGIYTPSD 423

Query: 431 HLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQIVDE 465
            L K+V +A+ERSGEKLW                    N G   GGSITA +FLKQ V E
Sbjct: 424 ELAKEVIAASERSGEKLWRMPLEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSE 483

BLAST of PI0003181 vs. ExPASy Swiss-Prot
Match: Q42876 (Leucine aminopeptidase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=LAPA2 PE=2 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.2e-117
Identity = 243/523 (46.46%), Postives = 323/523 (61.76%), Query Frame = 0

Query: 6   NIVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLH 65
           +I   T+GL +   S+APK +FAAK+ID +EWKGD+L VG TEKD+ +D NS F+NPLL 
Sbjct: 48  SIARDTLGLTHTNQSDAPKISFAAKEIDLVEWKGDILTVGATEKDLARDGNSKFQNPLLQ 107

Query: 66  KLDAIL-------------------------------RVGLFGLGQSASSATDFDGLGET 125
           KLD+ L                               R+ L GLG   SS   +  LGE 
Sbjct: 108 KLDSKLSGLLSEASSEEDFSGKAGQSTILRLPGLGSKRIALVGLGSPTSSTAAYRCLGEA 167

Query: 126 IAAAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRS 185
            AAA K++ A+++A+ALA ++ LS E K   AS I  G   G F+D+          L+S
Sbjct: 168 AAAAAKSAQASNIAIALASTDGLSAELKLSSASAITTGAVLGTFEDNRFKSESKKPTLKS 227

Query: 186 VDILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVL 245
           +DILGLG+GP+++KK+KYA  V +G++   EL N+PANVLT   L  E  K+   Y+DV 
Sbjct: 228 LDILGLGTGPEIEKKIKYAADVCAGVILGRELVNAPANVLTPAVLAEEAKKIASTYSDVF 287

Query: 246 SARIFDGEEIIEVKMGSFL---GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGG 305
           SA I D E+  E+KMGS+L     +ANP  FIHLCYKP SG +  K+ L GKG+TFDSGG
Sbjct: 288 SANILDVEQCKELKMGSYLRVAAASANPAHFIHLCYKPSSGEIKKKIALVGKGLTFDSGG 347

Query: 306 FNLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLN 365
           +N+KTG    IEL+K DMGGA+ +LGAAK++ +IKP GVEVHF+V A +NMIS TG    
Sbjct: 348 YNIKTGPGCSIELMKFDMGGAAAVLGAAKALGQIKPAGVEVHFIVAACENMISGTGMRPG 407

Query: 366 DIATTSNENKNEVNN---KGRFSLALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFK 425
           DI T SN    EVNN   +GR +L++  +  Q V+K ++L TLT A   A GP++ G+F 
Sbjct: 408 DIITASNGKTIEVNNTDAEGRLTLSVGISCNQGVEKIVDLATLTGACVVALGPSIAGIFT 467

Query: 426 PTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQI 465
           P++ L K+V +A+E SGEKLW                    N G   GG+ITA +FLKQ 
Sbjct: 468 PSDDLAKEVVAASEVSGEKLWRLPMEDSYWDSMKSGVADMVNTGGRPGGAITAALFLKQF 527

BLAST of PI0003181 vs. ExPASy TrEMBL
Match: A0A5D3BD80 (Leucine aminopeptidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00420 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 4.0e-156
Identity = 324/523 (61.95%), Postives = 364/523 (69.60%), Query Frame = 0

Query: 8   VPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKL 67
           +P T+GL  PG SNAPK TFAAKD D LEW GDLLAVGVTEKDVVKD+N+NF NPLL KL
Sbjct: 5   IPPTLGLTRPGPSNAPKITFAAKDTDVLEWNGDLLAVGVTEKDVVKDDNNNFTNPLLQKL 64

Query: 68  DAIL-------------------------------RVGLFGLGQSASSATDFDGLGETIA 127
           DA+L                               RVGLF LGQSAS A  F GLGE IA
Sbjct: 65  DALLGGLLAEASSEEDFSGKSGQAIVLRVSGLSFKRVGLFALGQSASRAAAFVGLGEAIA 124

Query: 128 AAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVD 187
           AA + S A SVA ALAFS DLSDE KP IAS IA+GI NGIFDD+        T+L+SVD
Sbjct: 125 AAAQASRAVSVAFALAFSADLSDEWKPAIASSIAIGIVNGIFDDNRYRSNPKATVLQSVD 184

Query: 188 ILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSA 247
           +LGLGSG  M K+LKYAQ VSSGI++V EL NSPAN+LT   L AEVL LT+ Y+DVLSA
Sbjct: 185 VLGLGSGAAMIKRLKYAQYVSSGIVFVRELVNSPANILTPGQLAAEVLNLTRNYSDVLSA 244

Query: 248 RIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGF 307
           RIFD EEIIE+KMGS+LG     TANPPKFIHLCYKPPSG VS KLGL GKGITFDSGG+
Sbjct: 245 RIFDEEEIIEMKMGSYLGVTAAATANPPKFIHLCYKPPSGSVSAKLGLVGKGITFDSGGY 304

Query: 308 NLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLND 367
           NLK G  S IE +KNDMGGA  I GAAK+IAEIKPLGVE+HFVV A +NMISATG   +D
Sbjct: 305 NLKAGSGSNIETMKNDMGGAGAIFGAAKAIAEIKPLGVEIHFVVAACENMISATGMRPSD 364

Query: 368 IATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVF 427
           I T +N    EVNN   +GR  L  ALI+  KQ VDK I+L TLT A  TA GP+V   F
Sbjct: 365 IVTAANGKTIEVNNTDAEGRLCLADALIYTCKQGVDKIIDLATLTGACITALGPSVAAAF 424

Query: 428 KPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQ 464
            P+E L  +V SAAERSGEKLW                    N G +QGG+ITA +FLKQ
Sbjct: 425 TPSEELANEVLSAAERSGEKLWRLPIEESYWESMKSGVADMVNTGPSQGGAITAALFLKQ 484

BLAST of PI0003181 vs. ExPASy TrEMBL
Match: A0A0A0LK31 (CYTOSOL_AP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G035470 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 1.2e-155
Identity = 322/523 (61.57%), Postives = 365/523 (69.79%), Query Frame = 0

Query: 8   VPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKL 67
           +P T+GL  PG SNAPK TFAAKD D LEW GDLLAVGVTEKDVVKD+N+NF N LL KL
Sbjct: 5   IPPTLGLTRPGPSNAPKITFAAKDTDVLEWNGDLLAVGVTEKDVVKDDNNNFTNSLLKKL 64

Query: 68  DAIL-------------------------------RVGLFGLGQSASSATDFDGLGETIA 127
           DA+L                               RVGLFGLGQSA SA  F GLGE IA
Sbjct: 65  DAVLGGLLAEASSEEDFSGKSGQAIVLRVSGLSFKRVGLFGLGQSAYSAAAFVGLGEAIA 124

Query: 128 AAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVD 187
           AA + S A S+AVALAFS DLSDESKP+IAS IA+GI NGIFDD+        T+L+SVD
Sbjct: 125 AAAQASRAVSLAVALAFSADLSDESKPDIASSIAIGIVNGIFDDNRYRSNPKATVLQSVD 184

Query: 188 ILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSA 247
           +LGLGSG  M KKLKYAQ VSSGI++V EL NSPAN+LT   L AEVL + + Y+DVLSA
Sbjct: 185 VLGLGSGAAMIKKLKYAQYVSSGIVFVRELVNSPANILTPGQLAAEVLNIARNYSDVLSA 244

Query: 248 RIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGF 307
           RIF+ +EIIE+KMGS+LG     TANPPKFIHLCYKPPSG VS KLGL GKGITFDSGG+
Sbjct: 245 RIFNEDEIIEMKMGSYLGVTAAATANPPKFIHLCYKPPSGCVSAKLGLVGKGITFDSGGY 304

Query: 308 NLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLND 367
           NLKTG  S IE +KNDMGGA  I GAAK+IAEIKPLGVE+HFVV A +NMISATG   +D
Sbjct: 305 NLKTGANSNIETMKNDMGGAGAIFGAAKAIAEIKPLGVEIHFVVAACENMISATGMRPSD 364

Query: 368 IATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVF 427
           I T +N    EVNN   +GR  L  ALI+  K  VDK I+L TLT A   A GP+V G F
Sbjct: 365 IVTAANGKTIEVNNTDAEGRLCLADALIYTCKLGVDKIIDLATLTGACIIALGPSVAGAF 424

Query: 428 KPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQ 464
            P E L  +V SAAERSGEKLW                    N G +QGG+ITA +FLKQ
Sbjct: 425 TPNEELASEVLSAAERSGEKLWRLPIEESYWDSMKSGVADMLNTGPSQGGAITAALFLKQ 484

BLAST of PI0003181 vs. ExPASy TrEMBL
Match: A0A6J1G4K2 (leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111450682 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 6.6e-151
Identity = 313/526 (59.51%), Postives = 364/526 (69.20%), Query Frame = 0

Query: 4   MPNIVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPL 63
           M +I   T+GL  PG SNAPK TF AKD+D LEW GDL+AVGV EKDV KDE  +FKNPL
Sbjct: 1   MADIARVTLGLTRPGPSNAPKITFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPL 60

Query: 64  LHKLDAIL-------------------------------RVGLFGLGQSASSATDFDGLG 123
           L++LD  L                               RVGLFGLGQSASSA  F GLG
Sbjct: 61  LYQLDEALGGLLGEASSEEDFSGKSAQSLVLRVSGLKFKRVGLFGLGQSASSAAAFVGLG 120

Query: 124 ETIAAAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLL 183
           E IAAA +TS AAS+A+ALAFSEDLS ESKP IAS IA+GI NGIFDD+       +T+L
Sbjct: 121 EAIAAAAQTSRAASLALALAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTVL 180

Query: 184 RSVDILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYND 243
           + VD+LGLG GP+M+KKLKYA+ VSSG+++ +EL NSPAN+LT   L  EVLK+T  YND
Sbjct: 181 QFVDVLGLGFGPNMEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYND 240

Query: 244 VLSARIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFD 303
           VLSA+IF+ EEIIE+KMGS+LG     TANPP FIHLCYKP  G VSTKLGL GKGITFD
Sbjct: 241 VLSAKIFNEEEIIEMKMGSYLGVTAAATANPPHFIHLCYKPLGGSVSTKLGLVGKGITFD 300

Query: 304 SGGFNLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGS 363
           SGG+NLKTG  S IE +KNDMGGA+ I GAAK+IAE+KP GVE+HFVV A +NMISATG 
Sbjct: 301 SGGYNLKTGANSNIETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGM 360

Query: 364 TLNDIATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAV 423
             +DI T SN    EVNN   +GR  L  ALI+     V+K I+L TLT A  TA GP+V
Sbjct: 361 RPSDIVTASNGKTIEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSV 420

Query: 424 GGVFKPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADM 463
            G F P E L K+V +AAERSGEK+W                    N G  QGG+ITA +
Sbjct: 421 AGAFTPNEELAKEVLAAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAAL 480

BLAST of PI0003181 vs. ExPASy TrEMBL
Match: A0A6J1KKF4 (leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111493959 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.9e-150
Identity = 311/527 (59.01%), Postives = 364/527 (69.07%), Query Frame = 0

Query: 4   MPNIVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPL 63
           M +I   T+GL  PG SNAPK +F AKD+D LEW GDL+AVGV EKDV KDE  +FKNPL
Sbjct: 1   MADIARVTLGLTRPGPSNAPKISFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPL 60

Query: 64  LHKLDAIL-------------------------------RVGLFGLGQSASSATDFDGLG 123
           L++L+  L                               RVGLFGLGQSASSA  F GLG
Sbjct: 61  LYQLNEALGGLLAEASSEEDFSGKSAQSLVLRVSGLRFKRVGLFGLGQSASSAAAFVGLG 120

Query: 124 ETIAAAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLL 183
           E IAAA + S AAS+A++LAFSEDLS ESKP IAS IA+GI NGIFDD+       +TLL
Sbjct: 121 EAIAAAAQASRAASLALSLAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTLL 180

Query: 184 RSVDILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYND 243
           +SVD+LGLG GP+M+KKLKYA+ VSSG+++ +EL NSPAN+LT   L  EVLK+T  Y+D
Sbjct: 181 QSVDVLGLGFGPNMEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYHD 240

Query: 244 VLSARIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFD 303
           VLSA+IF+ EEIIE+KMGS+LG     TANPP FIHLCYKPP G VSTKLGL GKGITFD
Sbjct: 241 VLSAKIFNEEEIIEMKMGSYLGVTAAATANPPHFIHLCYKPPGGSVSTKLGLVGKGITFD 300

Query: 304 SGGFNLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGS 363
           SGG+NLKTG  S IE +KNDMGGA+ I GAAK+IAE+KP GVE+HFVV A +NMISATG 
Sbjct: 301 SGGYNLKTGANSNIETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGM 360

Query: 364 TLNDIATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAV 423
             +DI T SN    EVNN   +GR  L  ALI+     V+K I+L TLT A  TA GP+V
Sbjct: 361 RPSDIVTASNGKTIEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSV 420

Query: 424 GGVFKPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADM 464
            G F P E L K+V  AAERSGEK+W                    N G  QGG+ITA +
Sbjct: 421 AGAFTPNEELAKEVIDAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAAL 480

BLAST of PI0003181 vs. ExPASy TrEMBL
Match: A0A6J1G2Q1 (leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111450256 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 4.9e-146
Identity = 307/530 (57.92%), Postives = 355/530 (66.98%), Query Frame = 0

Query: 1   MSFMPNIVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFK 60
           M+  PN    T+GL  PG SNAPK  FAAKDID LEW GDLLAVGV EKDV +DE  +FK
Sbjct: 1   MADTPN---DTLGLTRPGPSNAPKINFAAKDIDVLEWSGDLLAVGVIEKDVERDEEGHFK 60

Query: 61  NPLLHKLDAIL-------------------------------RVGLFGLGQSASSATDFD 120
           NPLLH+L+  L                               RVGLFGLGQSAS A  F 
Sbjct: 61  NPLLHRLNEALGGLLGEASSEEEFSGKSAQSIVLRVSGLRFKRVGLFGLGQSASRAVAFV 120

Query: 121 GLGETIAAAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------I 180
           GLGE IAAA + S AAS+AV LAFSEDLSDESK +IAS IAVGI NGIFDD+       +
Sbjct: 121 GLGEAIAAAAQASRAASLAVVLAFSEDLSDESKLDIASAIAVGIVNGIFDDNRYRSDPKM 180

Query: 181 TLLRSVDILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQM 240
           TLL SV++LGLGSGPDM+KKL+YA+ VSSGI++V+EL NSPANVLT   L  EV K+ + 
Sbjct: 181 TLLESVNVLGLGSGPDMEKKLEYAEYVSSGIVFVKELVNSPANVLTPGELAEEVSKIAEK 240

Query: 241 YNDVLSARIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGI 300
           YNDVLSA+IF  + +IE+KMGS+LG     TANP  FIHLCYKPP GPV TKLGL GKGI
Sbjct: 241 YNDVLSAKIFKEDRMIELKMGSYLGVTAAATANPAHFIHLCYKPPCGPVLTKLGLVGKGI 300

Query: 301 TFDSGGFNLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISA 360
           TFDSGG+NLKTG  S IE +KNDMGGA+ I GAAK+IA++KP GVE+HFVVPA +NMISA
Sbjct: 301 TFDSGGYNLKTGPNSSIETMKNDMGGAAAIFGAAKAIAQLKPPGVEIHFVVPACENMISA 360

Query: 361 TGSTLNDIATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARG 420
           TG   +DI T SN    EVNN   +GR  L  AL +     V+K I+L TLT A   A G
Sbjct: 361 TGMRPSDIVTASNGKTIEVNNTDAEGRLCLADALFYTCNLGVEKIIDLATLTGACIIALG 420

Query: 421 PAVGGVFKPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSIT 464
           P+V G F   E L K+V  AAERSGEK+W                    N G  QGG+IT
Sbjct: 421 PSVAGAFTQNEDLAKEVIDAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAIT 480

BLAST of PI0003181 vs. NCBI nr
Match: KAA0056102.1 (leucine aminopeptidase 1-like [Cucumis melo var. makuwa] >TYJ96415.1 leucine aminopeptidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 561.2 bits (1445), Expect = 8.2e-156
Identity = 324/523 (61.95%), Postives = 364/523 (69.60%), Query Frame = 0

Query: 8   VPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKL 67
           +P T+GL  PG SNAPK TFAAKD D LEW GDLLAVGVTEKDVVKD+N+NF NPLL KL
Sbjct: 5   IPPTLGLTRPGPSNAPKITFAAKDTDVLEWNGDLLAVGVTEKDVVKDDNNNFTNPLLQKL 64

Query: 68  DAIL-------------------------------RVGLFGLGQSASSATDFDGLGETIA 127
           DA+L                               RVGLF LGQSAS A  F GLGE IA
Sbjct: 65  DALLGGLLAEASSEEDFSGKSGQAIVLRVSGLSFKRVGLFALGQSASRAAAFVGLGEAIA 124

Query: 128 AAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVD 187
           AA + S A SVA ALAFS DLSDE KP IAS IA+GI NGIFDD+        T+L+SVD
Sbjct: 125 AAAQASRAVSVAFALAFSADLSDEWKPAIASSIAIGIVNGIFDDNRYRSNPKATVLQSVD 184

Query: 188 ILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSA 247
           +LGLGSG  M K+LKYAQ VSSGI++V EL NSPAN+LT   L AEVL LT+ Y+DVLSA
Sbjct: 185 VLGLGSGAAMIKRLKYAQYVSSGIVFVRELVNSPANILTPGQLAAEVLNLTRNYSDVLSA 244

Query: 248 RIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGF 307
           RIFD EEIIE+KMGS+LG     TANPPKFIHLCYKPPSG VS KLGL GKGITFDSGG+
Sbjct: 245 RIFDEEEIIEMKMGSYLGVTAAATANPPKFIHLCYKPPSGSVSAKLGLVGKGITFDSGGY 304

Query: 308 NLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLND 367
           NLK G  S IE +KNDMGGA  I GAAK+IAEIKPLGVE+HFVV A +NMISATG   +D
Sbjct: 305 NLKAGSGSNIETMKNDMGGAGAIFGAAKAIAEIKPLGVEIHFVVAACENMISATGMRPSD 364

Query: 368 IATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVF 427
           I T +N    EVNN   +GR  L  ALI+  KQ VDK I+L TLT A  TA GP+V   F
Sbjct: 365 IVTAANGKTIEVNNTDAEGRLCLADALIYTCKQGVDKIIDLATLTGACITALGPSVAAAF 424

Query: 428 KPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQ 464
            P+E L  +V SAAERSGEKLW                    N G +QGG+ITA +FLKQ
Sbjct: 425 TPSEELANEVLSAAERSGEKLWRLPIEESYWESMKSGVADMVNTGPSQGGAITAALFLKQ 484

BLAST of PI0003181 vs. NCBI nr
Match: XP_004144796.3 (leucine aminopeptidase 2, chloroplastic [Cucumis sativus] >KGN61072.2 hypothetical protein Csa_021227 [Cucumis sativus])

HSP 1 Score: 560.1 bits (1442), Expect = 1.8e-155
Identity = 322/523 (61.57%), Postives = 365/523 (69.79%), Query Frame = 0

Query: 8   VPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKL 67
           +P T+GL  PG SNAPK TFAAKD D LEW GDLLAVGVTEKDVVKD+N+NF N LL KL
Sbjct: 5   IPPTLGLTRPGPSNAPKITFAAKDTDVLEWNGDLLAVGVTEKDVVKDDNNNFTNSLLKKL 64

Query: 68  DAIL-------------------------------RVGLFGLGQSASSATDFDGLGETIA 127
           DA+L                               RVGLFGLGQSA SA  F GLGE IA
Sbjct: 65  DAVLGGLLAEASSEEDFSGKSGQAIVLRVSGLSFKRVGLFGLGQSAYSAAAFVGLGEAIA 124

Query: 128 AAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVD 187
           AA + S A S+AVALAFS DLSDESKP+IAS IA+GI NGIFDD+        T+L+SVD
Sbjct: 125 AAAQASRAVSLAVALAFSADLSDESKPDIASSIAIGIVNGIFDDNRYRSNSKATVLQSVD 184

Query: 188 ILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSA 247
           +LGLGSG    KKLKYAQ VSSGI++V EL NSPAN+LT   L AEVL +T+ Y+DVLSA
Sbjct: 185 VLGLGSGASTIKKLKYAQYVSSGIVFVRELVNSPANILTPGQLAAEVLNITRNYSDVLSA 244

Query: 248 RIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGF 307
           RIF+ +EIIE+KMGS+LG     TANPPKFIHLCYKPPSG VS KLGL GKGITFDSGG+
Sbjct: 245 RIFNEDEIIEMKMGSYLGVTAAATANPPKFIHLCYKPPSGCVSAKLGLVGKGITFDSGGY 304

Query: 308 NLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLND 367
           NLKTG  S IE +KNDMGGA  I GAAK+IAEIKPLGVE+HFVV A +NMISATG   +D
Sbjct: 305 NLKTGANSNIETMKNDMGGAGAIFGAAKAIAEIKPLGVEIHFVVAACENMISATGMRPSD 364

Query: 368 IATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVF 427
           I T +N    EVNN   +GR  L  ALI+  K  VDK I+L TLT A   A GP+V G F
Sbjct: 365 IVTAANGKTIEVNNTDAEGRLCLADALIYTCKLGVDKIIDLATLTGACIIALGPSVAGAF 424

Query: 428 KPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQ 464
            P E L  +V SAAERSGEKLW                    N G +QGG+ITA +FLKQ
Sbjct: 425 TPNEELASEVLSAAERSGEKLWRLPMEESYWDSMKSGVADMLNTGPSQGGAITAALFLKQ 484

BLAST of PI0003181 vs. NCBI nr
Match: XP_031736427.1 (leucine aminopeptidase 1 [Cucumis sativus] >KAE8651637.1 hypothetical protein Csa_021322 [Cucumis sativus])

HSP 1 Score: 557.4 bits (1435), Expect = 1.2e-154
Identity = 321/523 (61.38%), Postives = 365/523 (69.79%), Query Frame = 0

Query: 8   VPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKL 67
           +P T+GL  PG SNAPK TFAAKD D LEW GDLLAVGVTEKDVVKD+N+NF N LL KL
Sbjct: 5   IPPTLGLTNPGPSNAPKITFAAKDTDVLEWNGDLLAVGVTEKDVVKDDNNNFTNLLLKKL 64

Query: 68  DAIL-------------------------------RVGLFGLGQSASSATDFDGLGETIA 127
           DA+L                               RVGLFG+GQSA  A  F GLGE IA
Sbjct: 65  DAVLGGLLAEASSEEDFSGKSGQAIVLRVSGLCFKRVGLFGVGQSAYKAALFVGLGEAIA 124

Query: 128 AAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVD 187
           AA + S A S+AVALAFS DLSDESKP+IAS IA+GI NGIFDD+        T+L+SVD
Sbjct: 125 AAAQASRAVSLAVALAFSADLSDESKPDIASSIAIGIVNGIFDDNRYRSNPKATVLQSVD 184

Query: 188 ILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSA 247
           +LGLGSG  M KKLKYAQ VSSGI++V EL NSPAN+LT   L AEVL + + Y+DVLSA
Sbjct: 185 VLGLGSGAAMIKKLKYAQYVSSGIVFVRELVNSPANILTPGQLAAEVLNIARNYSDVLSA 244

Query: 248 RIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGF 307
           RIF+ +EIIE+KMGS+LG     TANPPKFIHLCYKPPSG VS KLGL GKGITFDSGG+
Sbjct: 245 RIFNEDEIIEMKMGSYLGVTAAATANPPKFIHLCYKPPSGSVSAKLGLVGKGITFDSGGY 304

Query: 308 NLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLND 367
           NLK G  S IE +KNDMGGA TI GAAK+IAEIKPLGVE+HFVV A +NMISATG   +D
Sbjct: 305 NLKAGSGSNIETMKNDMGGAGTIFGAAKAIAEIKPLGVEIHFVVAACENMISATGMRPSD 364

Query: 368 IATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVF 427
           I T +N    EVNN   +GR  L  AL++  KQ V K I+L TLT A  TA GP+V G F
Sbjct: 365 IVTAANGKTIEVNNTDAEGRLCLADALLYTCKQGVAKIIDLATLTGACITALGPSVAGAF 424

Query: 428 KPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQ 464
            P E L  +V SAAERSGEKLW                    N G +QGG+ITA +FLKQ
Sbjct: 425 TPNEELASEVLSAAERSGEKLWRLPIEESYWDSMKSGVADMLNTGPSQGGAITAALFLKQ 484

BLAST of PI0003181 vs. NCBI nr
Match: XP_022946689.1 (leucine aminopeptidase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 543.9 bits (1400), Expect = 1.4e-150
Identity = 313/526 (59.51%), Postives = 364/526 (69.20%), Query Frame = 0

Query: 4   MPNIVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPL 63
           M +I   T+GL  PG SNAPK TF AKD+D LEW GDL+AVGV EKDV KDE  +FKNPL
Sbjct: 1   MADIARVTLGLTRPGPSNAPKITFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPL 60

Query: 64  LHKLDAIL-------------------------------RVGLFGLGQSASSATDFDGLG 123
           L++LD  L                               RVGLFGLGQSASSA  F GLG
Sbjct: 61  LYQLDEALGGLLGEASSEEDFSGKSAQSLVLRVSGLKFKRVGLFGLGQSASSAAAFVGLG 120

Query: 124 ETIAAAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLL 183
           E IAAA +TS AAS+A+ALAFSEDLS ESKP IAS IA+GI NGIFDD+       +T+L
Sbjct: 121 EAIAAAAQTSRAASLALALAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTVL 180

Query: 184 RSVDILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYND 243
           + VD+LGLG GP+M+KKLKYA+ VSSG+++ +EL NSPAN+LT   L  EVLK+T  YND
Sbjct: 181 QFVDVLGLGFGPNMEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYND 240

Query: 244 VLSARIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFD 303
           VLSA+IF+ EEIIE+KMGS+LG     TANPP FIHLCYKP  G VSTKLGL GKGITFD
Sbjct: 241 VLSAKIFNEEEIIEMKMGSYLGVTAAATANPPHFIHLCYKPLGGSVSTKLGLVGKGITFD 300

Query: 304 SGGFNLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGS 363
           SGG+NLKTG  S IE +KNDMGGA+ I GAAK+IAE+KP GVE+HFVV A +NMISATG 
Sbjct: 301 SGGYNLKTGANSNIETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGM 360

Query: 364 TLNDIATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAV 423
             +DI T SN    EVNN   +GR  L  ALI+     V+K I+L TLT A  TA GP+V
Sbjct: 361 RPSDIVTASNGKTIEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSV 420

Query: 424 GGVFKPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADM 463
            G F P E L K+V +AAERSGEK+W                    N G  QGG+ITA +
Sbjct: 421 AGAFTPNEELAKEVLAAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAAL 480

BLAST of PI0003181 vs. NCBI nr
Match: XP_022999678.1 (leucine aminopeptidase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 542.3 bits (1396), Expect = 3.9e-150
Identity = 311/527 (59.01%), Postives = 364/527 (69.07%), Query Frame = 0

Query: 4   MPNIVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPL 63
           M +I   T+GL  PG SNAPK +F AKD+D LEW GDL+AVGV EKDV KDE  +FKNPL
Sbjct: 1   MADIARVTLGLTRPGPSNAPKISFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPL 60

Query: 64  LHKLDAIL-------------------------------RVGLFGLGQSASSATDFDGLG 123
           L++L+  L                               RVGLFGLGQSASSA  F GLG
Sbjct: 61  LYQLNEALGGLLAEASSEEDFSGKSAQSLVLRVSGLRFKRVGLFGLGQSASSAAAFVGLG 120

Query: 124 ETIAAAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLL 183
           E IAAA + S AAS+A++LAFSEDLS ESKP IAS IA+GI NGIFDD+       +TLL
Sbjct: 121 EAIAAAAQASRAASLALSLAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTLL 180

Query: 184 RSVDILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYND 243
           +SVD+LGLG GP+M+KKLKYA+ VSSG+++ +EL NSPAN+LT   L  EVLK+T  Y+D
Sbjct: 181 QSVDVLGLGFGPNMEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYHD 240

Query: 244 VLSARIFDGEEIIEVKMGSFLG----DTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFD 303
           VLSA+IF+ EEIIE+KMGS+LG     TANPP FIHLCYKPP G VSTKLGL GKGITFD
Sbjct: 241 VLSAKIFNEEEIIEMKMGSYLGVTAAATANPPHFIHLCYKPPGGSVSTKLGLVGKGITFD 300

Query: 304 SGGFNLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGS 363
           SGG+NLKTG  S IE +KNDMGGA+ I GAAK+IAE+KP GVE+HFVV A +NMISATG 
Sbjct: 301 SGGYNLKTGANSNIETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGM 360

Query: 364 TLNDIATTSNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAV 423
             +DI T SN    EVNN   +GR  L  ALI+     V+K I+L TLT A  TA GP+V
Sbjct: 361 RPSDIVTASNGKTIEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSV 420

Query: 424 GGVFKPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADM 464
            G F P E L K+V  AAERSGEK+W                    N G  QGG+ITA +
Sbjct: 421 AGAFTPNEELAKEVIDAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAAL 480

BLAST of PI0003181 vs. TAIR 10
Match: AT4G30920.1 (Cytosol aminopeptidase family protein )

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-125
Identity = 261/520 (50.19%), Postives = 332/520 (63.85%), Query Frame = 0

Query: 11  TIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKLDAI 70
           T+GL    S + PK +F+ K+ID  EWKGD+LAVGVTEKD+ KD NS F+NP+L KLDA 
Sbjct: 65  TLGLTQANSVDHPKISFSGKEIDVTEWKGDILAVGVTEKDMAKDVNSKFENPILKKLDAH 124

Query: 71  L-------------------------------RVGLFGLGQSASSATDFDGLGETIAAAG 130
           L                               RVGL GLG+SAS+ + F  LGE +AAA 
Sbjct: 125 LGGLLADVSSEEDFSGKPGQSTVLRLPGLGSKRVGLIGLGKSASTPSAFQSLGEAVAAAA 184

Query: 131 KTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSVDILG 190
           K S A+SVAV LA SE +S+ESK   AS IA G   G+F+D           L+SVDI+G
Sbjct: 185 KASQASSVAVVLASSESVSNESKLCSASAIASGTVLGLFEDSRYKSESKKPSLKSVDIIG 244

Query: 191 LGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARIF 250
            GSGP+++KKLKYA+ VS G+++ +EL NSPANVLT   L  E L L  MY+DV++A I 
Sbjct: 245 FGSGPELEKKLKYAEHVSYGVIFGKELVNSPANVLTPAVLAEEALNLASMYSDVMTANIL 304

Query: 251 DGEEIIEVKMGSFL---GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNLKT 310
           + E+  E+KMGS+L     +ANPP FIHL YKP SGPV TKL L GKG+TFDSGG+N+KT
Sbjct: 305 NEEQCKELKMGSYLAVAAASANPPHFIHLIYKPSSGPVKTKLALVGKGLTFDSGGYNIKT 364

Query: 311 GGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIATT 370
           G    IEL+K DMGG++ +LGAAK+I +IKP GVEVHF+V A +NMIS TG    D+ T 
Sbjct: 365 GPGCLIELMKFDMGGSAAVLGAAKAIGQIKPPGVEVHFIVAACENMISGTGMRPGDVLTA 424

Query: 371 SNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKPTE 430
           SN    EVNN   +GR +L  AL++A  Q VDK ++L TLT A   A G ++ G++ P++
Sbjct: 425 SNGKTIEVNNTDAEGRLTLADALVYACNQGVDKVVDLATLTGACIIALGTSMAGIYTPSD 484

Query: 431 HLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQIVDE 465
            L K+V +A+ERSGEKLW                    N G   GGSITA +FLKQ V E
Sbjct: 485 KLAKEVIAASERSGEKLWRMPMEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSE 544

BLAST of PI0003181 vs. TAIR 10
Match: AT2G24200.1 (Cytosol aminopeptidase family protein )

HSP 1 Score: 428.7 bits (1101), Expect = 5.9e-120
Identity = 251/520 (48.27%), Postives = 320/520 (61.54%), Query Frame = 0

Query: 11  TIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKLDAI 70
           T+GL  P S+   K +F AK+ID +EWKGD+L VGVTEKD+ KD NS F+NP+L K+DA 
Sbjct: 4   TLGLTQPNSTEPHKISFTAKEIDVIEWKGDILVVGVTEKDLAKDGNSKFENPILSKVDAH 63

Query: 71  L-------------------------------RVGLFGLGQSASSATDFDGLGETIAAAG 130
           L                               R+ L GLGQS SS   F  LGE +A   
Sbjct: 64  LSGLLAQVSSEEDFTGKPGQSTVLRLPGLGSKRIALIGLGQSVSSPVAFHSLGEAVATVS 123

Query: 131 KTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDD-------DITLLRSVDILG 190
           K S + S A+ LA S  +SDESK    S +A GI  G+F+D           L++VDI+G
Sbjct: 124 KASQSTSAAIVLASS--VSDESKLSSVSALASGIVLGLFEDGRYKSESKKPSLKAVDIIG 183

Query: 191 LGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARIF 250
            G+G +++KKLKYA+ VS G+++  EL NSPANVLT   L  E  K+   Y+DV +A I 
Sbjct: 184 FGTGAEVEKKLKYAEDVSYGVIFGRELINSPANVLTPAVLAEEAAKVASTYSDVFTANIL 243

Query: 251 DGEEIIEVKMGSFL---GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNLKT 310
           + E+  E+KMGS+L     +ANPP FIHL YKPP+G V TKL L GKG+TFDSGG+N+KT
Sbjct: 244 NEEQCKELKMGSYLAVAAASANPPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKT 303

Query: 311 GGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIATT 370
           G    IEL+K DMGG++ +LGAAK+I EIKP GVEVHF+V A +NMIS TG    D+ T 
Sbjct: 304 GPGCSIELMKFDMGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITA 363

Query: 371 SNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKPTE 430
           SN    EVNN   +GR +L  AL++A  Q VDK ++L TLT A   A G ++ G++ P++
Sbjct: 364 SNGKTIEVNNTDAEGRLTLADALVYACNQGVDKIVDLATLTGACVIALGTSMAGIYTPSD 423

Query: 431 HLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQIVDE 465
            L K+V +A+ERSGEKLW                    N G   GGSITA +FLKQ V E
Sbjct: 424 ELAKEVIAASERSGEKLWRMPLEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSE 483

BLAST of PI0003181 vs. TAIR 10
Match: AT2G24200.2 (Cytosol aminopeptidase family protein )

HSP 1 Score: 428.7 bits (1101), Expect = 5.9e-120
Identity = 251/520 (48.27%), Postives = 320/520 (61.54%), Query Frame = 0

Query: 11  TIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKLDAI 70
           T+GL  P S+   K +F AK+ID +EWKGD+L VGVTEKD+ KD NS F+NP+L K+DA 
Sbjct: 4   TLGLTQPNSTEPHKISFTAKEIDVIEWKGDILVVGVTEKDLAKDGNSKFENPILSKVDAH 63

Query: 71  L-------------------------------RVGLFGLGQSASSATDFDGLGETIAAAG 130
           L                               R+ L GLGQS SS   F  LGE +A   
Sbjct: 64  LSGLLAQVSSEEDFTGKPGQSTVLRLPGLGSKRIALIGLGQSVSSPVAFHSLGEAVATVS 123

Query: 131 KTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDD-------DITLLRSVDILG 190
           K S + S A+ LA S  +SDESK    S +A GI  G+F+D           L++VDI+G
Sbjct: 124 KASQSTSAAIVLASS--VSDESKLSSVSALASGIVLGLFEDGRYKSESKKPSLKAVDIIG 183

Query: 191 LGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARIF 250
            G+G +++KKLKYA+ VS G+++  EL NSPANVLT   L  E  K+   Y+DV +A I 
Sbjct: 184 FGTGAEVEKKLKYAEDVSYGVIFGRELINSPANVLTPAVLAEEAAKVASTYSDVFTANIL 243

Query: 251 DGEEIIEVKMGSFL---GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNLKT 310
           + E+  E+KMGS+L     +ANPP FIHL YKPP+G V TKL L GKG+TFDSGG+N+KT
Sbjct: 244 NEEQCKELKMGSYLAVAAASANPPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKT 303

Query: 311 GGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIATT 370
           G    IEL+K DMGG++ +LGAAK+I EIKP GVEVHF+V A +NMIS TG    D+ T 
Sbjct: 304 GPGCSIELMKFDMGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITA 363

Query: 371 SNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKPTE 430
           SN    EVNN   +GR +L  AL++A  Q VDK ++L TLT A   A G ++ G++ P++
Sbjct: 364 SNGKTIEVNNTDAEGRLTLADALVYACNQGVDKIVDLATLTGACVIALGTSMAGIYTPSD 423

Query: 431 HLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQIVDE 465
            L K+V +A+ERSGEKLW                    N G   GGSITA +FLKQ V E
Sbjct: 424 ELAKEVIAASERSGEKLWRMPLEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSE 483

BLAST of PI0003181 vs. TAIR 10
Match: AT4G30910.1 (Cytosol aminopeptidase family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-118
Identity = 249/523 (47.61%), Postives = 323/523 (61.76%), Query Frame = 0

Query: 7   IVPQTIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHK 66
           I   T+GL    S + PK +F+ K+ID  EWKGD+LAVGVTEKD+ KD NS F+NP+L K
Sbjct: 60  IAHATLGLTQANSVDHPKISFSGKEIDVTEWKGDILAVGVTEKDMAKDANSKFENPILKK 119

Query: 67  LDAIL-------------------------------RVGLFGLGQSASSATDFDGLGETI 126
           LDA L                               RVGL GLG+SAS+ + F  LGE +
Sbjct: 120 LDAHLGGLLADVSAEEDFSGKPGQSTVLRLPGLGSKRVGLIGLGKSASTPSAFQSLGEAV 179

Query: 127 AAAGKTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDDD-------ITLLRSV 186
           AAA K S A+SVAV LA SE  SDESK   AS IA G   G+F+D           L+SV
Sbjct: 180 AAAAKASQASSVAVVLASSESFSDESKLSSASDIASGTVLGLFEDSRYKSESKKPSLKSV 239

Query: 187 DILGLGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLS 246
             +G G+GP+++ KLKYA+ VS G+++ +EL NSPANVL+   L  E   L  MY++V++
Sbjct: 240 VFIGFGTGPELENKLKYAEHVSYGVIFTKELVNSPANVLSPAVLAEEASNLASMYSNVMT 299

Query: 247 ARIFDGEEIIEVKMGSFL---GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGF 306
           A I   E+  E+KMGS+L     +ANPP FIHL YKP SGPV TKL L GKG+TFDSGG+
Sbjct: 300 ANILKEEQCKELKMGSYLAVAAASANPPHFIHLIYKPSSGPVKTKLALVGKGLTFDSGGY 359

Query: 307 NLKTGGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLND 366
           N+K G    IEL+K D+GG++ +LGAAK+I EIKP GVEVHF+V A +NMIS TG    D
Sbjct: 360 NIKIGPELIIELMKIDVGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGD 419

Query: 367 IATTSNENKNEVN---NKGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVF 426
           + T SN    EVN   ++GR +L  AL++A  Q VDK +++ TLT     A GP++ G++
Sbjct: 420 VITASNGKTIEVNDTDSEGRLTLADALVYACNQGVDKIVDIATLTGEIIVALGPSMAGMY 479

Query: 427 KPTEHLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQ 464
             ++ L K+V +A++RSGEKLW                    N G   GGSITA +FLK+
Sbjct: 480 TASDELAKEVIAASQRSGEKLWRMPMEESYWEMMKSGVADMVNFGGRAGGSITAALFLKR 539

BLAST of PI0003181 vs. TAIR 10
Match: AT2G24200.3 (Cytosol aminopeptidase family protein )

HSP 1 Score: 374.0 bits (959), Expect = 1.7e-103
Identity = 231/520 (44.42%), Postives = 294/520 (56.54%), Query Frame = 0

Query: 11  TIGLNYPGSSNAPKATFAAKDIDALEWKGDLLAVGVTEKDVVKDENSNFKNPLLHKLDAI 70
           T+GL  P S+   K +F AK+ID +EWKGD+L VGVTEKD+ KD NS F+NP+L K+DA 
Sbjct: 4   TLGLTQPNSTEPHKISFTAKEIDVIEWKGDILVVGVTEKDLAKDGNSKFENPILSKVDAH 63

Query: 71  L-------------------------------RVGLFGLGQSASSATDFDGLGETIAAAG 130
           L                               R+ L GLGQS SS   F  LGE +A   
Sbjct: 64  LSGLLAQVSSEEDFTGKPGQSTVLRLPGLGSKRIALIGLGQSVSSPVAFHSLGEAVATVS 123

Query: 131 KTSGAASVAVALAFSEDLSDESKPEIASIIAVGIANGIFDD-------DITLLRSVDILG 190
           K S + S A+ LA S  +SDESK    S +A GI  G+F+D           L++VDI+G
Sbjct: 124 KASQSTSAAIVLASS--VSDESKLSSVSALASGIVLGLFEDGRYKSESKKPSLKAVDIIG 183

Query: 191 LGSGPDMDKKLKYAQSVSSGIMYVEELENSPANVLTTETLTAEVLKLTQMYNDVLSARIF 250
            G+G +                                    E  K+   Y+DV +A I 
Sbjct: 184 FGTGAE------------------------------------EAAKVASTYSDVFTANIL 243

Query: 251 DGEEIIEVKMGSFL---GDTANPPKFIHLCYKPPSGPVSTKLGLAGKGITFDSGGFNLKT 310
           + E+  E+KMGS+L     +ANPP FIHL YKPP+G V TKL L GKG+TFDSGG+N+KT
Sbjct: 244 NEEQCKELKMGSYLAVAAASANPPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKT 303

Query: 311 GGYSKIELLKNDMGGASTILGAAKSIAEIKPLGVEVHFVVPASKNMISATGSTLNDIATT 370
           G    IEL+K DMGG++ +LGAAK+I EIKP GVEVHF+V A +NMIS TG    D+ T 
Sbjct: 304 GPGCSIELMKFDMGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITA 363

Query: 371 SNENKNEVNN---KGRFSL--ALIHASKQDVDKNIELPTLTDAWTTARGPAVGGVFKPTE 430
           SN    EVNN   +GR +L  AL++A  Q VDK ++L TLT A   A G ++ G++ P++
Sbjct: 364 SNGKTIEVNNTDAEGRLTLADALVYACNQGVDKIVDLATLTGACVIALGTSMAGIYTPSD 423

Query: 431 HLGKKVTSAAERSGEKLW--------------------NAGSAQGGSITADMFLKQIVDE 465
            L K+V +A+ERSGEKLW                    N G   GGSITA +FLKQ V E
Sbjct: 424 ELAKEVIAASERSGEKLWRMPLEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSE 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q944P71.7e-12450.19Leucine aminopeptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LAP2 ... [more]
P314273.1e-12148.08Leucine aminopeptidase, chloroplastic OS=Solanum tuberosum OX=4113 GN=LAP PE=2 S... [more]
Q107124.9e-11947.78Leucine aminopeptidase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=LAPA1... [more]
P301848.3e-11948.27Leucine aminopeptidase 1 OS=Arabidopsis thaliana OX=3702 GN=LAP1 PE=1 SV=1[more]
Q428761.2e-11746.46Leucine aminopeptidase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=LAPA2... [more]
Match NameE-valueIdentityDescription
A0A5D3BD804.0e-15661.95Leucine aminopeptidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A0A0LK311.2e-15561.57CYTOSOL_AP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G035470 ... [more]
A0A6J1G4K26.6e-15159.51leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KKF41.9e-15059.01leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1G2Q14.9e-14657.92leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
KAA0056102.18.2e-15661.95leucine aminopeptidase 1-like [Cucumis melo var. makuwa] >TYJ96415.1 leucine ami... [more]
XP_004144796.31.8e-15561.57leucine aminopeptidase 2, chloroplastic [Cucumis sativus] >KGN61072.2 hypothetic... [more]
XP_031736427.11.2e-15461.38leucine aminopeptidase 1 [Cucumis sativus] >KAE8651637.1 hypothetical protein Cs... [more]
XP_022946689.11.4e-15059.51leucine aminopeptidase 2, chloroplastic-like [Cucurbita moschata][more]
XP_022999678.13.9e-15059.01leucine aminopeptidase 2, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G30920.11.2e-12550.19Cytosol aminopeptidase family protein [more]
AT2G24200.15.9e-12048.27Cytosol aminopeptidase family protein [more]
AT2G24200.25.9e-12048.27Cytosol aminopeptidase family protein [more]
AT4G30910.12.5e-11847.61Cytosol aminopeptidase family protein [more]
AT2G24200.31.7e-10344.42Cytosol aminopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011356Peptidase M17, leucine aminopeptidase/peptidase BPRINTSPR00481LAMNOPPTDASEcoord: 275..296
score: 42.76
coord: 251..268
score: 65.23
coord: 313..334
score: 30.98
IPR011356Peptidase M17, leucine aminopeptidase/peptidase BPANTHERPTHR11963LEUCINE AMINOPEPTIDASE-RELATEDcoord: 14..71
IPR011356Peptidase M17, leucine aminopeptidase/peptidase BPANTHERPTHR11963LEUCINE AMINOPEPTIDASE-RELATEDcoord: 72..463
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 161..461
e-value: 1.8E-65
score: 223.2
NoneNo IPR availablePANTHERPTHR11963:SF41LEUCINE AMINOPEPTIDASE 2, CHLOROPLASTIC-RELATEDcoord: 72..463
NoneNo IPR availablePANTHERPTHR11963:SF41LEUCINE AMINOPEPTIDASE 2, CHLOROPLASTIC-RELATEDcoord: 14..71
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 170..460
IPR008283Peptidase M17, leucyl aminopeptidase, N-terminalPFAMPF02789Peptidase_M17_Ncoord: 70..141
e-value: 4.0E-5
score: 23.5
IPR000819Peptidase M17, leucyl aminopeptidase, C-terminalPFAMPF00883Peptidase_M17coord: 177..403
e-value: 1.4E-43
score: 149.4
IPR043472Macro domain-likeGENE3D3.40.220.10Leucine Aminopeptidase, subunit E, domain 1coord: 4..71
e-value: 1.5E-12
score: 49.5
coord: 72..160
e-value: 6.4E-12
score: 47.4
IPR043472Macro domain-likeSUPERFAMILY52949Macro domain-likecoord: 27..172

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003181.1PI0003181.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0019538 protein metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0070006 metalloaminopeptidase activity