Homology
BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match:
Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)
HSP 1 Score: 623.6 bits (1607), Expect = 2.2e-177
Identity = 327/551 (59.35%), Postives = 402/551 (72.96%), Query Frame = 0
Query: 20 PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPR 79
P+ + DP L+++++ +I F +L+ VV I R S P+
Sbjct: 8 PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPK 67
Query: 80 --NKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTS 139
KP+QAISR CS +LYP LC+ +LL FPG+ +A+ ++L+HIS N TLQ +KALY++S
Sbjct: 68 LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127
Query: 140 QIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFST-----EASNHDIMTWLSAALTY 199
I Q+ P SAY+ C+ELL+D++DA + +L S + S+ D+MTWLS+A+T
Sbjct: 128 TITYTQM--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187
Query: 200 HDTCTAGFEDVADLG--VKDEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRL 259
HDTCT GF+++ G VKD+V + DLSEM+SN LAIF+G +PV N R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKL 247
Query: 260 MESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIE 319
+ GT + E P W+ +DR LL P SAIQADI V+KDGSG FKT+AEAI+
Sbjct: 248 L--GTE--------ETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307
Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
AP S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367
Query: 380 ATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
ATFA +G G I+RDMTFEN AGP+KHQAVALR+ DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427
Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487
Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
+AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEY 543
BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match:
Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)
HSP 1 Score: 612.5 bits (1578), Expect = 5.0e-174
Identity = 313/556 (56.29%), Postives = 405/556 (72.84%), Query Frame = 0
Query: 13 SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSRIA 72
S + +TT A P +++ R + ++++ S +++ LI A+ + A + R ++
Sbjct: 14 SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73
Query: 73 SSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSA-NVHDLVHISLNLTLQHLT 132
S P R KPSQAIS+AC T +P LC+ SL+ FPG+ +A + DL+H+++N+TL H +
Sbjct: 74 QSVPGLAR-KPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133
Query: 133 KALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSTEASNHDIMTWLS 192
ALYS++ + + P A SAY+ C+ELL+D++DA S S+ S+ A D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193
Query: 193 AALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
AALT HDTCT GF+ V D GVKD + A L +LSE++SN LAIFS GD +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253
Query: 253 KRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTV 312
+ + E E FP W+ K+R +L P+S IQADI+V+KDG+G KT+
Sbjct: 254 RLLGVEER------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313
Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
+EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373
Query: 373 TTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
TTF TA+FA +G G I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433
Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493
Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
HA +++A DL+A+ GS T+LGRPWKL+SR VYM+S +G H+H RGWLEW +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553
Query: 553 YYGEYMNSGPGPPWGR 560
YYGEY+NSGPG G+
Sbjct: 554 YYGEYLNSGPGSGLGQ 554
BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match:
O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)
HSP 1 Score: 409.1 bits (1050), Expect = 8.4e-113
Identity = 231/551 (41.92%), Postives = 339/551 (61.52%), Query Frame = 0
Query: 37 SLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISR-ACSHTLY 96
+ ++ +++++S LL A+V + + + + + S A+ R +CS T Y
Sbjct: 13 NFKKNKKLVLLSAAVALLFVAAVAG----ISAGASKANEKRTLSPSSHAVLRSSCSSTRY 72
Query: 97 PTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 156
P LC+S++++ G + + D++ S+NLT+ + ++ ++ + P +A
Sbjct: 73 PELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALH 132
Query: 157 DCMELLNDAIDAF-----SLSLFSTEAS--NH--DIMTWLSAALTYHDTCTAGF-EDVAD 216
DC+E +++ +D L L+ T+ + H D+ T +S+A+T +TC GF D AD
Sbjct: 133 DCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDAD 192
Query: 217 LGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRKRRRLMESGTPSWAA 276
V+ + + M SN+LA+ D+ + + R+ E+ + A
Sbjct: 193 KQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAV 252
Query: 277 ENGG----DHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSS 336
+ G D EG+P W+S DRRLL S ++AD VA DGSG FKTVA A+ AAP +S
Sbjct: 253 DIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENS 312
Query: 337 GRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGS 396
+R +I+IKAG Y EN++V +KK N+MF+GDG+ T+I+G ++V D TTF +AT A
Sbjct: 313 NKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 372
Query: 397 GTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDI 456
G + RD+TF+NTAGPSKHQAVALR+ +D + Y+C+++ YQDTLYVHSNRQFF +C I
Sbjct: 373 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 432
Query: 457 YGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDL 516
GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I C+I AT DL
Sbjct: 433 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 492
Query: 517 EASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 566
++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 493 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGA- 552
BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match:
O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)
HSP 1 Score: 407.9 bits (1047), Expect = 1.9e-112
Identity = 239/554 (43.14%), Postives = 336/554 (60.65%), Query Frame = 0
Query: 25 RLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQ 84
+LS + S++ ++ + L ++ + LL+ A+V+ V SR S N+P
Sbjct: 10 KLSESSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHH 69
Query: 85 AISR-ACSHTLYPTLCLSSLLSFPGAQS--ANVHDLVHISLNLTLQHLTKALYSTSQIPI 144
AI + +CS T YP LC S++ + P A + D++ +SLN+T + + ++ +
Sbjct: 70 AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-L 129
Query: 145 LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTE-------ASNH--DIMTWLSAALTY 204
+ + A DC+E +++ +D ++ E S H D+ T +SAA+T
Sbjct: 130 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 189
Query: 205 HDTCTAGF-EDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMES 264
TC GF D A+ V+D + + +M SN+LA+ D+ + R +
Sbjct: 190 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKL-- 249
Query: 265 GTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAP 324
E +G+P W+S DRRLL + S++ + VVA DGSG FKTVA A+ AAP
Sbjct: 250 ------TEETSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAP 309
Query: 325 SSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATF 384
+R II IKAG Y EN++V +K N+MF+GDG+ T+I+G ++V D TTF++AT
Sbjct: 310 QGGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATA 369
Query: 385 AGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRE 444
A G G + RD+TF+NTAGPSKHQAVALR+ AD + Y+C+++ YQDTLYVHSNRQFF
Sbjct: 370 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 429
Query: 445 CDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVAT 504
C I GT+DFIFGNA V Q+C+I+ARKP +GQKN +TAQ R DPNQNTGI I +I AT
Sbjct: 430 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGAT 489
Query: 505 GDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNS 564
DL+ +GSFPT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ NS
Sbjct: 490 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNS 544
Query: 565 GPGPPWGRGSSGRV 566
G G G+SGRV
Sbjct: 550 GA----GAGTSGRV 544
BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match:
P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)
HSP 1 Score: 407.1 bits (1045), Expect = 3.2e-112
Identity = 237/554 (42.78%), Postives = 337/554 (60.83%), Query Frame = 0
Query: 25 RLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQ 84
+LS + S++ ++ + L ++ + LL+ A+V+ V SR S N+P
Sbjct: 10 KLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHH 69
Query: 85 AISR-ACSHTLYPTLCLSSLLSFPGAQS--ANVHDLVHISLNLTLQHLTKALYSTSQIPI 144
AI + +CS T YP LC S++ + P A + D++ +SLN+T + + ++ +
Sbjct: 70 AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-L 129
Query: 145 LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTE-------ASNH--DIMTWLSAALTY 204
+ + A DC+E +++ +D ++ E S H D+ T +SAA+T
Sbjct: 130 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 189
Query: 205 HDTCTAGF-EDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMES 264
TC GF D A+ V+D + + +M SN+LA+ D+ + R +
Sbjct: 190 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKL-- 249
Query: 265 GTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAP 324
E +G+P W+S DRRLL + S++ ++VVA DGSG FKTVA ++ AAP
Sbjct: 250 ------IEETSTVDGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAP 309
Query: 325 SSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATF 384
+R II IKAG Y EN++V +K N+MF+GDG+ T+I+G ++V D TTF++AT
Sbjct: 310 QGGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 369
Query: 385 AGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRE 444
A G G + RD+TF+NTAGPSKHQAVALR+ AD + Y+C+++ YQDTLYVHSNRQFF
Sbjct: 370 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 429
Query: 445 CDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVAT 504
C I GT+DFIFGNA V Q+C+I+ARKP +GQKN +TAQ R DPNQNTGI I +I AT
Sbjct: 430 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGAT 489
Query: 505 GDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNS 564
DL+ +GSFPT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N+
Sbjct: 490 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNA 544
Query: 565 GPGPPWGRGSSGRV 566
G G G+SGRV
Sbjct: 550 GA----GAGTSGRV 544
BLAST of PI0003165 vs. ExPASy TrEMBL
Match:
A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)
HSP 1 Score: 1067.4 bits (2759), Expect = 2.1e-308
Identity = 537/559 (96.06%), Postives = 547/559 (97.85%), Query Frame = 0
Query: 1 MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
MNNNN+YNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1 MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
Query: 61 SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 120
SAAILVRS + SSQPHFP NKPSQAISRACSHTLYP+LCLSSLLSFPGAQSA+VHDLVHI
Sbjct: 61 SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 180
SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS +ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
Query: 181 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
DIMTWLSAALTYHDTCTAGF+DVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
Query: 241 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 300
ENRKRRRLMES T SWAAENGGDHEGFP W+SGKDRRLLAAP+S IQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
Query: 361 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
DKVTTFRTATFAGSGT IILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
Query: 541 DTLYYGEYMNSGPGPPWGR 560
DTLYYGEYMNSGPG G+
Sbjct: 541 DTLYYGEYMNSGPGAAVGQ 559
BLAST of PI0003165 vs. ExPASy TrEMBL
Match:
A0A1S3AUZ6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1)
HSP 1 Score: 1060.8 bits (2742), Expect = 2.0e-306
Identity = 539/560 (96.25%), Postives = 547/560 (97.68%), Query Frame = 0
Query: 1 MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
MNNNNNY +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1 MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
Query: 61 SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 120
SAAILVRSRIASSQPHFP NKPSQAISRACSHTLYP+LCLSSLLSFPGAQSA+VHDLVHI
Sbjct: 61 SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 180
SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFST ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180
Query: 181 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
DIMTWLSAALTYHDTCT GF+DV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
Query: 241 ENRKRRRLMESGTP-SWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGK 300
ENRKRRRLMESGTP SWAAENGGDHEGFPEW+SGKDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300
Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360
Query: 361 YDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
YDKVTTFRTATFAGSGTGIILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420
Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480
Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
Query: 541 LDTLYYGEYMNSGPGPPWGR 560
LDTLYYGEYMNSGPG G+
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQ 560
BLAST of PI0003165 vs. ExPASy TrEMBL
Match:
A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)
HSP 1 Score: 926.0 bits (2392), Expect = 7.5e-266
Identity = 475/559 (84.97%), Postives = 509/559 (91.06%), Query Frame = 0
Query: 4 NNNYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
NNNYN LH+SHS + TPA+SFR+S +DP PP S R RIL+ISF S+LLI AS VS
Sbjct: 2 NNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61
Query: 64 AAILVRSRI-ASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 123
A +LV SRI AS++ HFP NKP+QAISRACS TLYP+LCLSSLLS PG+ SA+VHDLVHI
Sbjct: 62 AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121
Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 183
SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181
Query: 184 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
D+MTWLSAALTYHDTCT GF DVAD +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241
Query: 244 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 303
ENR+RRRLMES A EN GD + FP WVSGKDRRLLAAPIS+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301
Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361
Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADHAVV+ N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421
Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481
Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
ISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541
Query: 544 DTLYYGEYMNSGPGPPWGR 560
DTLYYGEYMNSGPG G+
Sbjct: 542 DTLYYGEYMNSGPGAAVGQ 557
BLAST of PI0003165 vs. ExPASy TrEMBL
Match:
A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)
HSP 1 Score: 914.4 bits (2362), Expect = 2.3e-262
Identity = 471/562 (83.81%), Postives = 505/562 (89.86%), Query Frame = 0
Query: 4 NNNYNKLHTSHSANTTPAASFRLSGN-----DPPPPSSSLRRRSRILIISFMSVLLIAAS 63
NNNYNKLH+S S + TP SFR+S + PPPPSSS R RIL+ISF S+LLI AS
Sbjct: 2 NNNYNKLHSSRSGD-TPVTSFRVSNDHSNPPPPPPPSSSHR---RILLISFFSILLITAS 61
Query: 64 VVSAAILVRSRI-ASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDL 123
VSA +LV SRI S++ HFP NKP+QAISRACS TLYP+LC+SSLLS PG+ SA+VHDL
Sbjct: 62 TVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDL 121
Query: 124 VHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEA 183
VHISLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A
Sbjct: 122 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 181
Query: 184 SNHDIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD 243
+NHD+MTWLSAALTYHDTCT GF DVAD VKD++ AKLSDLSEMISNSLAIFSGFGGGD
Sbjct: 182 TNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGGD 241
Query: 244 LPVENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGS 303
LPVENR+RRRLMES A EN GD + FP WVSGKDRRLLAAPIS+IQADIVVAKDGS
Sbjct: 242 LPVENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGS 301
Query: 304 GKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 363
GKFKTVAEAI AAPSSS RRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 302 GKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 361
Query: 364 SVYDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 423
SVYDKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADH VV+ N+IGYQ
Sbjct: 362 SVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQ 421
Query: 424 DTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQ 483
DTLYVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQ
Sbjct: 422 DTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQ 481
Query: 484 NTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 543
NTGISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 482 NTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGS 541
Query: 544 FALDTLYYGEYMNSGPGPPWGR 560
FALDTLYYGEYMNSGP G+
Sbjct: 542 FALDTLYYGEYMNSGPSAAVGQ 557
BLAST of PI0003165 vs. ExPASy TrEMBL
Match:
A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)
HSP 1 Score: 888.3 bits (2294), Expect = 1.7e-254
Identity = 463/557 (83.12%), Postives = 500/557 (89.77%), Query Frame = 0
Query: 5 NNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAI 64
NNYNKLH+S S +T A+F++S PP SS+ RRR+RIL +S +S+LLI AS VSAA+
Sbjct: 2 NNYNKLHSSASGDT--PATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAV 61
Query: 65 LVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHISLNL 124
L+ S+ HFP NKP+QAIS ACSHTLYP+LC SSLLSFPG+ SA+VH LVHISLNL
Sbjct: 62 LLHSQ----SSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNL 121
Query: 125 TLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNHDIMT 184
TLQHLTKALY++SQIP+ QIS +PLAHSAY+DC+ELLNDA+DAFSLSLFST ASNHDIMT
Sbjct: 122 TLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMT 181
Query: 185 WLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRK 244
WLSAALTYHDTC+ GF DV D VKD++ A+LSDLSEMISNSLAIFSG+G G LPV+N
Sbjct: 182 WLSAALTYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGYGEG-LPVQN-- 241
Query: 245 RRRLMESGTPSWAAENG-GDHEG-FPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 304
RRRLMES WAAENG EG FP WVSGKDRRLLAAPISAIQADIVVAKDGSGKFKT
Sbjct: 242 RRRLMESDR-EWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 301
Query: 305 VAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDK 364
VAEAIEAAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+DK
Sbjct: 302 VAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDK 361
Query: 365 VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYV 424
VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALR+SADHAVVY CNIIGYQDTLYV
Sbjct: 362 VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYV 421
Query: 425 HSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGIS 484
HSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM QKNTITAQ+RKDPNQNTGIS
Sbjct: 422 HSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGIS 481
Query: 485 IHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT 544
IHACKI+AT DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT
Sbjct: 482 IHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT 541
Query: 545 LYYGEYMNSGPGPPWGR 560
LYYGEYMNSGPG G+
Sbjct: 542 LYYGEYMNSGPGAATGQ 547
BLAST of PI0003165 vs. NCBI nr
Match:
XP_011654619.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.1 hypothetical protein Csa_000423 [Cucumis sativus])
HSP 1 Score: 1067.4 bits (2759), Expect = 4.3e-308
Identity = 537/559 (96.06%), Postives = 547/559 (97.85%), Query Frame = 0
Query: 1 MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
MNNNN+YNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1 MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
Query: 61 SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 120
SAAILVRS + SSQPHFP NKPSQAISRACSHTLYP+LCLSSLLSFPGAQSA+VHDLVHI
Sbjct: 61 SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 180
SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS +ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
Query: 181 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
DIMTWLSAALTYHDTCTAGF+DVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
Query: 241 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 300
ENRKRRRLMES T SWAAENGGDHEGFP W+SGKDRRLLAAP+S IQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
Query: 361 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
DKVTTFRTATFAGSGT IILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
Query: 541 DTLYYGEYMNSGPGPPWGR 560
DTLYYGEYMNSGPG G+
Sbjct: 541 DTLYYGEYMNSGPGAAVGQ 559
BLAST of PI0003165 vs. NCBI nr
Match:
XP_008437596.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])
HSP 1 Score: 1060.8 bits (2742), Expect = 4.0e-306
Identity = 539/560 (96.25%), Postives = 547/560 (97.68%), Query Frame = 0
Query: 1 MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
MNNNNNY +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1 MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
Query: 61 SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 120
SAAILVRSRIASSQPHFP NKPSQAISRACSHTLYP+LCLSSLLSFPGAQSA+VHDLVHI
Sbjct: 61 SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 180
SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFST ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180
Query: 181 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
DIMTWLSAALTYHDTCT GF+DV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
Query: 241 ENRKRRRLMESGTP-SWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGK 300
ENRKRRRLMESGTP SWAAENGGDHEGFPEW+SGKDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300
Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360
Query: 361 YDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
YDKVTTFRTATFAGSGTGIILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420
Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480
Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
Query: 541 LDTLYYGEYMNSGPGPPWGR 560
LDTLYYGEYMNSGPG G+
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQ 560
BLAST of PI0003165 vs. NCBI nr
Match:
XP_038874841.1 (probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida])
HSP 1 Score: 988.4 bits (2554), Expect = 2.5e-284
Identity = 503/559 (89.98%), Postives = 528/559 (94.45%), Query Frame = 0
Query: 4 NNNYNKLHTSHSANTTPAASFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63
NNNYNKLH+S SA +TPA+SF LSGND PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 3 NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62
Query: 64 SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 123
SAA+LVRSRIAS++PHFP NKPSQAISRACSHTLYP+LCLSSLLS P A SA+VHDLVHI
Sbjct: 63 SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122
Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 183
SLNLTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFS+SLFSTEASNH
Sbjct: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182
Query: 184 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
D+MTWLSAALTYHDTCT GF DVADL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242
Query: 244 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 303
+NR RRRLMESGT SWAAENGGD GFP+W+S KDRRLLAAPISAIQADIVVAKDGSGKF
Sbjct: 243 QNR-RRRLMESGTRSWAAENGGD-GGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 302
Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
KTVAEAI+AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+
Sbjct: 303 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 362
Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
DKVTTFRTATFAGSGTGIILRDM FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDTL
Sbjct: 363 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 422
Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
YVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNTG
Sbjct: 423 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 482
Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
ISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFAL
Sbjct: 483 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 542
Query: 544 DTLYYGEYMNSGPGPPWGR 560
DTLYYGEY NSGPG G+
Sbjct: 543 DTLYYGEYSNSGPGAAVGQ 559
BLAST of PI0003165 vs. NCBI nr
Match:
XP_022922302.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])
HSP 1 Score: 926.0 bits (2392), Expect = 1.6e-265
Identity = 475/559 (84.97%), Postives = 509/559 (91.06%), Query Frame = 0
Query: 4 NNNYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
NNNYN LH+SHS + TPA+SFR+S +DP PP S R RIL+ISF S+LLI AS VS
Sbjct: 2 NNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61
Query: 64 AAILVRSRI-ASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 123
A +LV SRI AS++ HFP NKP+QAISRACS TLYP+LCLSSLLS PG+ SA+VHDLVHI
Sbjct: 62 AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121
Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 183
SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181
Query: 184 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
D+MTWLSAALTYHDTCT GF DVAD +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241
Query: 244 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 303
ENR+RRRLMES A EN GD + FP WVSGKDRRLLAAPIS+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301
Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361
Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADHAVV+ N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421
Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481
Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
ISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541
Query: 544 DTLYYGEYMNSGPGPPWGR 560
DTLYYGEYMNSGPG G+
Sbjct: 542 DTLYYGEYMNSGPGAAVGQ 557
BLAST of PI0003165 vs. NCBI nr
Match:
KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 924.5 bits (2388), Expect = 4.5e-265
Identity = 474/559 (84.79%), Postives = 509/559 (91.06%), Query Frame = 0
Query: 4 NNNYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
NNNYNKLH+S S + TPA+SFR+S +DP PP S R RIL+ISF+S+ LI AS VS
Sbjct: 2 NNNYNKLHSSRSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFLSIFLITASAVS 61
Query: 64 AAILVRSRI-ASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 123
A +LV SRI AS++ HFP NKP+QAISRACS TLYP+LCLSSLLS PG+ SA+VHDLVHI
Sbjct: 62 AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121
Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 183
SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181
Query: 184 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
D+MTWLSAALTYHDTCT GF DVAD +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241
Query: 244 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 303
ENR+RRRLMES A EN GD + FP+WVSGKDRRLLAAPIS+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEHWLAEENNGDGD-FPQWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301
Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361
Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADHAVV+ N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421
Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
YVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481
Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
ISIHACKI+ATGDLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541
Query: 544 DTLYYGEYMNSGPGPPWGR 560
DTLYYGEYMNSGPG G+
Sbjct: 542 DTLYYGEYMNSGPGAAVGQ 557
BLAST of PI0003165 vs. TAIR 10
Match:
AT5G53370.1 (pectin methylesterase PCR fragment F )
HSP 1 Score: 623.6 bits (1607), Expect = 1.5e-178
Identity = 327/551 (59.35%), Postives = 402/551 (72.96%), Query Frame = 0
Query: 20 PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPR 79
P+ + DP L+++++ +I F +L+ VV I R S P+
Sbjct: 8 PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPK 67
Query: 80 --NKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTS 139
KP+QAISR CS +LYP LC+ +LL FPG+ +A+ ++L+HIS N TLQ +KALY++S
Sbjct: 68 LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127
Query: 140 QIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFST-----EASNHDIMTWLSAALTY 199
I Q+ P SAY+ C+ELL+D++DA + +L S + S+ D+MTWLS+A+T
Sbjct: 128 TITYTQM--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187
Query: 200 HDTCTAGFEDVADLG--VKDEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRL 259
HDTCT GF+++ G VKD+V + DLSEM+SN LAIF+G +PV N R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKL 247
Query: 260 MESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIE 319
+ GT + E P W+ +DR LL P SAIQADI V+KDGSG FKT+AEAI+
Sbjct: 248 L--GTE--------ETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307
Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
AP S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367
Query: 380 ATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
ATFA +G G I+RDMTFEN AGP+KHQAVALR+ DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427
Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487
Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
+AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEY 543
BLAST of PI0003165 vs. TAIR 10
Match:
AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 612.5 bits (1578), Expect = 3.5e-175
Identity = 313/556 (56.29%), Postives = 405/556 (72.84%), Query Frame = 0
Query: 13 SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSRIA 72
S + +TT A P +++ R + ++++ S +++ LI A+ + A + R ++
Sbjct: 14 SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73
Query: 73 SSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSA-NVHDLVHISLNLTLQHLT 132
S P R KPSQAIS+AC T +P LC+ SL+ FPG+ +A + DL+H+++N+TL H +
Sbjct: 74 QSVPGLAR-KPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133
Query: 133 KALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSTEASNHDIMTWLS 192
ALYS++ + + P A SAY+ C+ELL+D++DA S S+ S+ A D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193
Query: 193 AALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
AALT HDTCT GF+ V D GVKD + A L +LSE++SN LAIFS GD +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253
Query: 253 KRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTV 312
+ + E E FP W+ K+R +L P+S IQADI+V+KDG+G KT+
Sbjct: 254 RLLGVEER------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313
Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
+EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373
Query: 373 TTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
TTF TA+FA +G G I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433
Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493
Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
HA +++A DL+A+ GS T+LGRPWKL+SR VYM+S +G H+H RGWLEW +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553
Query: 553 YYGEYMNSGPGPPWGR 560
YYGEY+NSGPG G+
Sbjct: 554 YYGEYLNSGPGSGLGQ 554
BLAST of PI0003165 vs. TAIR 10
Match:
AT3G14310.1 (pectin methylesterase 3 )
HSP 1 Score: 409.1 bits (1050), Expect = 5.9e-114
Identity = 231/551 (41.92%), Postives = 339/551 (61.52%), Query Frame = 0
Query: 37 SLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISR-ACSHTLY 96
+ ++ +++++S LL A+V + + + + + S A+ R +CS T Y
Sbjct: 13 NFKKNKKLVLLSAAVALLFVAAVAG----ISAGASKANEKRTLSPSSHAVLRSSCSSTRY 72
Query: 97 PTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 156
P LC+S++++ G + + D++ S+NLT+ + ++ ++ + P +A
Sbjct: 73 PELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALH 132
Query: 157 DCMELLNDAIDAF-----SLSLFSTEAS--NH--DIMTWLSAALTYHDTCTAGF-EDVAD 216
DC+E +++ +D L L+ T+ + H D+ T +S+A+T +TC GF D AD
Sbjct: 133 DCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDAD 192
Query: 217 LGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRKRRRLMESGTPSWAA 276
V+ + + M SN+LA+ D+ + + R+ E+ + A
Sbjct: 193 KQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAV 252
Query: 277 ENGG----DHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSS 336
+ G D EG+P W+S DRRLL S ++AD VA DGSG FKTVA A+ AAP +S
Sbjct: 253 DIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENS 312
Query: 337 GRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGS 396
+R +I+IKAG Y EN++V +KK N+MF+GDG+ T+I+G ++V D TTF +AT A
Sbjct: 313 NKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 372
Query: 397 GTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDI 456
G + RD+TF+NTAGPSKHQAVALR+ +D + Y+C+++ YQDTLYVHSNRQFF +C I
Sbjct: 373 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 432
Query: 457 YGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDL 516
GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I C+I AT DL
Sbjct: 433 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 492
Query: 517 EASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 566
++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 493 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGA- 552
BLAST of PI0003165 vs. TAIR 10
Match:
AT1G53830.1 (pectin methylesterase 2 )
HSP 1 Score: 406.0 bits (1042), Expect = 5.0e-113
Identity = 231/543 (42.54%), Postives = 321/543 (59.12%), Query Frame = 0
Query: 36 SSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISRACSHTLY 95
S + ++++ S LL+ AS+V A ++ + + + + CS TLY
Sbjct: 12 SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLY 71
Query: 96 PTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 155
P LC S++ + G + + +++ SLNLT + + ++ ++ + P +A
Sbjct: 72 PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 131
Query: 156 DCMELLNDAIDAFSLSL---------FSTEASNHDIMTWLSAALTYHDTCTAGFE-DVAD 215
DC+E +++ +D +++ S D+ T +S+A+T TC GF D AD
Sbjct: 132 DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDAD 191
Query: 216 LGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESGTPSWAAENGG-- 275
V+ + + M SN+LA+ D+ E R + + E G
Sbjct: 192 RKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDL 251
Query: 276 DHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYI 335
D +G+P+W+S DRRLL S I+AD VA DGSG F TVA A+ AAP S +R +I+I
Sbjct: 252 DSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHI 311
Query: 336 KAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIILRD 395
KAG Y EN++V +KKTN+MF+GDG+G T+I+G ++V D TTF +AT A G + RD
Sbjct: 312 KAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARD 371
Query: 396 MTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIF 455
+TF+NTAGPSKHQAVALR+ +D + Y C++ YQDTLYVHSNRQFF +C I GT+DFIF
Sbjct: 372 ITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIF 431
Query: 456 GNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFP 515
GNA V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I C+I T DL A KG+FP
Sbjct: 432 GNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFP 491
Query: 516 TFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGPPWGRGSS 566
T+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G G G++
Sbjct: 492 TYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRG----GGAGTA 547
BLAST of PI0003165 vs. TAIR 10
Match:
AT1G11580.1 (methylesterase PCR A )
HSP 1 Score: 372.9 bits (956), Expect = 4.7e-103
Identity = 221/529 (41.78%), Postives = 302/529 (57.09%), Query Frame = 0
Query: 37 SLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYP 96
SL+ ++ L++SF+++L A + I V + N S + H +
Sbjct: 14 SLKHKNLCLVLSFVAILGSVAFFTAQLISVNTN---------NNDDSLLTTSQICHGAHD 73
Query: 97 TLCLSSLLS---FPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSA 156
+LLS N DL+H+ L ++ L + S+ I S +
Sbjct: 74 QDSCQALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIR--SNGVRDKAG 133
Query: 157 YEDCMELLNDAIDAFSLSLFSTEASNH------DIMTWLSAALTYHDTCTAGFEDVADLG 216
+ DC E+++ + D S+ N+ ++ TWLS+ LT + TC DV+ +
Sbjct: 134 FADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVS-VN 193
Query: 217 VKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESGTPSWAAENGGDHEGF 276
K V+ +L DL +LAIF LP + + + F
Sbjct: 194 SKQIVKPQLEDLVSRARVALAIFVSV----LPARDDLKMIISNR---------------F 253
Query: 277 PEWVSGKDRRLLAAPISAIQ--ADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYIKAG 336
P W++ DR+LL + ++ A++VVAKDG+GKFKTV EA+ AAP +S R +IY+K G
Sbjct: 254 PSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKG 313
Query: 337 KYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIILRDMTF 396
Y +E + +G+KK NLM VGDGK T+I+G +V D TTFR+AT A +G G + +D+ F
Sbjct: 314 VY-KETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWF 373
Query: 397 ENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIFGNA 456
+NTAGP+KHQAVALR+SAD V+ C I YQDTLY H+ RQF+R+ I GT+DFIFGN+
Sbjct: 374 QNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNS 433
Query: 457 VVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFPTFL 516
VVFQ+C+I AR P AGQKN +TAQ R+D NQNT ISI CKI A+ DL KGS TFL
Sbjct: 434 AVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFL 493
Query: 517 GRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 555
GRPWKLYSR V M S + +HI P GW W G FAL TLYYGEY N+GPG
Sbjct: 494 GRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPG 510
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9FK05 | 2.2e-177 | 59.35 | Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... | [more] |
Q9M3B0 | 5.0e-174 | 56.29 | Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... | [more] |
O49006 | 8.4e-113 | 41.92 | Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... | [more] |
O04886 | 1.9e-112 | 43.14 | Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1 | [more] |
P83948 | 3.2e-112 | 42.78 | Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0KJG1 | 2.1e-308 | 96.06 | Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1 | [more] |
A0A1S3AUZ6 | 2.0e-306 | 96.25 | Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1 | [more] |
A0A6J1E2V1 | 7.5e-266 | 84.97 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1 | [more] |
A0A6J1IA52 | 2.3e-262 | 83.81 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1 | [more] |
A0A6J1H2K1 | 1.7e-254 | 83.12 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_011654619.1 | 4.3e-308 | 96.06 | probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.... | [more] |
XP_008437596.1 | 4.0e-306 | 96.25 | PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... | [more] |
XP_038874841.1 | 2.5e-284 | 89.98 | probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida] | [more] |
XP_022922302.1 | 1.6e-265 | 84.97 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata] | [more] |
KAG6579321.1 | 4.5e-265 | 84.79 | putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... | [more] |