PI0003165 (gene) Melon (PI 482460) v1

Overview
NamePI0003165
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectinesterase
Locationchr09: 266362 .. 271453 (-)
RNA-Seq ExpressionPI0003165
SyntenyPI0003165
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACTATATATGAAGAAGCCCTTTTCTTATTTAAACTCACTCCCACCATTATTCCCTCCCCATGCACACAAAATAACCAACCATGAATAACAATAACAACTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCTGCCTCCTTCCGCCTCTCCGGCAATGATCCCCCGCCACCCTCCTCCTCCCTCCGCCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCTGTCCTCCTCATTGCTGCTTCAGTCGTTTCCGCAGCTATCCTTGTTCGATCACGTATCGCATCAAGCCAACCCCATTTCCCTCGCAACAAGCCCTCCCAAGCCATCTCCCGCGCCTGTAGCCACACGCTTTACCCAACTCTTTGCCTCTCCTCTCTTCTATCTTTCCCCGGCGCGCAATCGGCCAACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAACATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCTATCTTACAAATCTCTAAGGACCCTTTGGCTCATTCTGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCTTTTTCTCTCTCTCTTTTCTCTACGGAAGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCATGACACGTGTACGGCTGGATTTGAGGATGTGGCTGACTTGGGTGTTAAGGATGAAGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCGGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTGGTACGCCGTCGTGGGCGGCGGAGAATGGTGGCGATCATGAGGGATTTCCTGAGTGGGTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCGATATCGGCGATTCAGGCGGATATTGTGGTAGCTAAGGATGGCTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCATCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTAAGAACTATACCTTTTCTAAATACTGTTAGGAAATCAACTCTCAGCCCAGTTATGTTTATTTTGGCATAACTATACTTTTCTCTAAACTATTCTCATATATATGTTTTCACTATTTAAATTATCCGTCCAGTCTAAACTATATCATTTCTATAAAAAAGATATATATAATATTTTAAATAATAAAAATTTATCACGTGCCAAATTATGAGTGGACTCTAGTGTTTAGTAGTAAGATACCTAAGTTATTTTTCTTAAAATTATAGTGTCTTACTATTTACAAGACTATATAAATATACTTTGTCCTTTGATTAATACATTTTGAAAGAAATGTAATAGGTTGTTTTCAAAACTTTAGTTTGAAGAAGATAGAAATCTAGGGGTATAGTTAGTTGGATATTTTAGAGAGTAGTGCATCATGATTTTCTTTTTAATTAATAAAGTTAATTAATCTTCTTCTTCTTTTTTACCTTTCAAAATTTCAAAATTTCAATGATTATTTGTTTATTTCTGTTTTTTCTTCTTCTTTTTCTTGGGAAAGTGGAGAAAAAAAATGGTTGTGATTAAGCCATAATTAATCAACATTAGGTCTCATTAAAAAGGAAGGGAAACAAGGAAAAAGAAAAGTTTTATTTTATAAACTTTTATCCTGATGTGTTTAATTTATTGACTTCAGTGTCATAATCATGCAAAGCTGTTACCTTACAAATTCCTAAAGTCCGTAGTTTTTTCATGATAATAATTATACAATCAAAACAATATTTTATATGTCCCCTCACAATAATTAATTTAAGTCCTTTTCAATTAAGTTGCTTTCATAATAATAACTCTTAATAACAACCAAATTAAAATCAATAAAATTCAACATGAATAAATATATTCAAATAAATCCCTAGTATAAGAAATTTTTTTAAAACGATATACGACAAATATGAAAATAGTTTAGAAAATGAAAGAGATAGGTTAGGAAAAATGATCCATCACTAGAATTATTAGTATATTTAAAGGCCAACATTTTTAGGAGAATTTATTTATTTATTTGGGTGTATTTGATAATAATTGAAAAATGGTGTGTGTGGGAAATTGGAATGGGAGTAAGAAAGGAGATAAATGTAATTTTGTAGGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGGTATATACTCTCTCACTCTCTTTCCCCCTTTTTTTTCTTCAATTTTATTCCCAAACCCCCCTTTTTTTTTTCTTTTATCTTTTTACTAAAAATTCTCTTCATATAATAAATAATTAATTTAAATAGTATTTTGGTCCCTTCCCTAATTTAAATGTCTAATTTTAGTCTAAACAAGTTTAAGATTTATTAAAAATGCCAAGTTAATAACTAAAGTTTTAATAGACACAAATTTTAATTTATTTTATTTTTAGTACAAATCAAGACTTTTAATTCTGACACCGGATATCTAGGACTTAGAATTATGGTCGTCCGGTCTTAGTACAAAAGTGGCCATACTAATTTTCTTCTTTTTCTACTTAAACTTTATTTTGTCTATTGAATTTTCCAAATTATATCATCCCAAATTATGTTTTTTATCTCTCCATTTTTAATAATAAATTAGTAAAACATTTGAATTCATAACTAAATTTAAAAAATTAATATAATTTTAATAAAATTAGAAGTTTAGATATAAAAATAATATTAATTTTTTTTAGACAAAAGAAAAAAAACAAAAATTCCTATATTATTATTATTATTATTATTAGTATTATCATTTTTTTTGGTTAAAAAAGGGTTATATTTCAAGATAGTGACAAATTAAGCTACTTTTATAGCATTTAATGGGCTACAAAAATAATTGTTTGATTTATATCCAAATAATCATATGAGAAATAGTAGATTCCAGCTGTGTTTGTCAAGTAGTCTTCATCGTTAATTATTTTTGTAGGAAAAAGCACCTTCCATTCATATTTTATAATTATATGCTATGCTAATGTATACGTTTACTAGTAAAGTCAAGCTTACAAATTGAATATCTGACGTAACATTTTCACATGACATATTATTAAATATATATCATAAAGATTTTCTTACTAGTAAAAGTATTATGAAAATTTGATCATCTTTAATTTTTTTAAAAAAATAAAGTACCATAAGATGGTAGTAATTAAGCAAGTTGTATTAATTGGTTATTATTTGTACTAAATTTTAAATTTTCATATTATAAGAAGATTCTAGTAGGGTTTTAAAAATCTTTTTGTTATCTCTATAAGAAAGATGATGTCTTTAATTATTATATAATATACTGTGCCTATCATAATTAATTGGTAAAGTAGGCCACTCTAATAAATTATTATAATAATCATTTTTTTTTTAAAGAAGATGAGAATTAATTTCTCTTTTTTAAAAAAAGGAATGGAATATCCCAATTATTCATGATGATTTAGAAAAGTCATAGTAGTATTATTCCCATGTTTCTTGCATCATGCTTACTTCCACAAGTTGGTAAAAAAGTTTTGTTTTTTATTTTGTTTCTTGTTTTTTAAAAAATGAGAATCTAATGTGGTCCAAATAAAGATGCCAAAGCTTACTGTCCATGGAGATAAGGATAGAAGTTAGAAAATAAATTATTGAATTTTATTTCAAAAGTTGTCTATTATCCCTCATCACATAATCTATGGTTCTCACCAATAAACTTATACAAGTAATTGATATTATATTTTTGTGGTAAATTATAAATTTAGCTTCTATCTTTTAAATGATGGTGTCCATGAGCACTTAAACTTTAACTGAGTTGAATATGAAAAATATATCTATGTACTATCTAAGTTGTATGTATCACATATGAAAAACTTTTAAATATTACAGTCAAAATAGGATTTAATATATATATATTTTAATTATAAATTGTGTCTAATAGATTATAAACTTTTTGTTACACAGCGGGTTCAGGAACAGGAATAATTCTACGAGATATGACGTTTGAGAACACCGCAGGACCAAGCAAGCACCAAGCAGTAGCATTACGCTTGAGTGCGGACCATGCCGTGGTATACCACTGCAACATCATTGGTTATCAAGATACTCTCTATGTGCACTCCAACCGTCAATTCTTTCGGGAGTGCGACATCTATGGAACAATCGACTTCATCTTCGGCAACGCTGTGGTGGTTTTCCAAAGCTGCAACATCTACGCCCGAAAGCCAATGGCAGGACAAAAAAACACGATCACAGCTCAAGACCGAAAGGACCCGAACCAAAACACCGGAATCTCAATCCACGCATGCAAGATCGTAGCCACCGGCGACCTTGAAGCTTCCAAGGGTTCATTCCCCACGTTTCTGGGTCGACCTTGGAAATTATACTCTAGAGTGGTGTATATGGTATCTTCCATGGGGGACCATATACATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTATATGAATTCTGGGCCGGGGCCGCCGTGGGGCAGAGGGTCAAGTGGCCGGGTTACCGGGTCATAACTTCCACGGTGGAAGCCTCCAAATTCACTGTTGGCCAGTTTATTTACGGTTCTTCTTGGTTGCCTTCTACAGGAGTCTCCTTTTCTGCTGGATTATCCACTAACTAAATATCCATATATATTATATTATATGTATTTAAATAATTTCTTTCACAAAATTTGTTTTTTTGGGTTTTTGTTATTTTTTTTAGGATGTGGTTCATTATGACACCTGTATAGTCTCATTCCTTTGATTATGCTCTTCCAAAATAATATGGGCCATACATCAAACTATGACAAATATTTGGATAGGATGGTCATTTATATATATACATATATACATACATACACATACACATATATATGGTTATAATTGTGATTGTATTTGTTGTATCAACTTTATATATTTTCAGAATAAATTTGTTTTCTCTTTTCCA

mRNA sequence

CAACTATATATGAAGAAGCCCTTTTCTTATTTAAACTCACTCCCACCATTATTCCCTCCCCATGCACACAAAATAACCAACCATGAATAACAATAACAACTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCTGCCTCCTTCCGCCTCTCCGGCAATGATCCCCCGCCACCCTCCTCCTCCCTCCGCCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCTGTCCTCCTCATTGCTGCTTCAGTCGTTTCCGCAGCTATCCTTGTTCGATCACGTATCGCATCAAGCCAACCCCATTTCCCTCGCAACAAGCCCTCCCAAGCCATCTCCCGCGCCTGTAGCCACACGCTTTACCCAACTCTTTGCCTCTCCTCTCTTCTATCTTTCCCCGGCGCGCAATCGGCCAACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAACATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCTATCTTACAAATCTCTAAGGACCCTTTGGCTCATTCTGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCTTTTTCTCTCTCTCTTTTCTCTACGGAAGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCATGACACGTGTACGGCTGGATTTGAGGATGTGGCTGACTTGGGTGTTAAGGATGAAGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCGGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTGGTACGCCGTCGTGGGCGGCGGAGAATGGTGGCGATCATGAGGGATTTCCTGAGTGGGTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCGATATCGGCGATTCAGGCGGATATTGTGGTAGCTAAGGATGGCTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCATCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGTTCAGGAACAGGAATAATTCTACGAGATATGACGTTTGAGAACACCGCAGGACCAAGCAAGCACCAAGCAGTAGCATTACGCTTGAGTGCGGACCATGCCGTGGTATACCACTGCAACATCATTGGTTATCAAGATACTCTCTATGTGCACTCCAACCGTCAATTCTTTCGGGAGTGCGACATCTATGGAACAATCGACTTCATCTTCGGCAACGCTGTGGTGGTTTTCCAAAGCTGCAACATCTACGCCCGAAAGCCAATGGCAGGACAAAAAAACACGATCACAGCTCAAGACCGAAAGGACCCGAACCAAAACACCGGAATCTCAATCCACGCATGCAAGATCGTAGCCACCGGCGACCTTGAAGCTTCCAAGGGTTCATTCCCCACGTTTCTGGGTCGACCTTGGAAATTATACTCTAGAGTGGTGTATATGGTATCTTCCATGGGGGACCATATACATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTATATGAATTCTGGGCCGGGGCCGCCGTGGGGCAGAGGGTCAAGTGGCCGGGTTACCGGGTCATAACTTCCACGGTGGAAGCCTCCAAATTCACTGTTGGCCAGTTTATTTACGGTTCTTCTTGGTTGCCTTCTACAGGAGTCTCCTTTTCTGCTGGATTATCCACTAACTAAATATCCATATATATTATATTATATGTATTTAAATAATTTCTTTCACAAAATTTGTTTTTTTGGGTTTTTGTTATTTTTTTTAGGATGTGGTTCATTATGACACCTGTATAGTCTCATTCCTTTGATTATGCTCTTCCAAAATAATATGGGCCATACATCAAACTATGACAAATATTTGGATAGGATGGTCATTTATATATATACATATATACATACATACACATACACATATATATGGTTATAATTGTGATTGTATTTGTTGTATCAACTTTATATATTTTCAGAATAAATTTGTTTTCTCTTTTCCA

Coding sequence (CDS)

ATGAATAACAATAACAACTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCTGCCTCCTTCCGCCTCTCCGGCAATGATCCCCCGCCACCCTCCTCCTCCCTCCGCCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCTGTCCTCCTCATTGCTGCTTCAGTCGTTTCCGCAGCTATCCTTGTTCGATCACGTATCGCATCAAGCCAACCCCATTTCCCTCGCAACAAGCCCTCCCAAGCCATCTCCCGCGCCTGTAGCCACACGCTTTACCCAACTCTTTGCCTCTCCTCTCTTCTATCTTTCCCCGGCGCGCAATCGGCCAACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAACATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCTATCTTACAAATCTCTAAGGACCCTTTGGCTCATTCTGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCTTTTTCTCTCTCTCTTTTCTCTACGGAAGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCATGACACGTGTACGGCTGGATTTGAGGATGTGGCTGACTTGGGTGTTAAGGATGAAGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCGGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTGGTACGCCGTCGTGGGCGGCGGAGAATGGTGGCGATCATGAGGGATTTCCTGAGTGGGTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCGATATCGGCGATTCAGGCGGATATTGTGGTAGCTAAGGATGGCTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCATCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGTTCAGGAACAGGAATAATTCTACGAGATATGACGTTTGAGAACACCGCAGGACCAAGCAAGCACCAAGCAGTAGCATTACGCTTGAGTGCGGACCATGCCGTGGTATACCACTGCAACATCATTGGTTATCAAGATACTCTCTATGTGCACTCCAACCGTCAATTCTTTCGGGAGTGCGACATCTATGGAACAATCGACTTCATCTTCGGCAACGCTGTGGTGGTTTTCCAAAGCTGCAACATCTACGCCCGAAAGCCAATGGCAGGACAAAAAAACACGATCACAGCTCAAGACCGAAAGGACCCGAACCAAAACACCGGAATCTCAATCCACGCATGCAAGATCGTAGCCACCGGCGACCTTGAAGCTTCCAAGGGTTCATTCCCCACGTTTCTGGGTCGACCTTGGAAATTATACTCTAGAGTGGTGTATATGGTATCTTCCATGGGGGACCATATACATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTATATGAATTCTGGGCCGGGGCCGCCGTGGGGCAGAGGGTCAAGTGGCCGGGTTACCGGGTCATAA

Protein sequence

MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNHDIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGPPWGRGSSGRVTGS
Homology
BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 2.2e-177
Identity = 327/551 (59.35%), Postives = 402/551 (72.96%), Query Frame = 0

Query: 20  PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPR 79
           P+     +  DP      L+++++  +I F   +L+   VV   I    R   S    P+
Sbjct: 8   PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPK 67

Query: 80  --NKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTS 139
              KP+QAISR CS +LYP LC+ +LL FPG+ +A+ ++L+HIS N TLQ  +KALY++S
Sbjct: 68  LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127

Query: 140 QIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFST-----EASNHDIMTWLSAALTY 199
            I   Q+   P   SAY+ C+ELL+D++DA + +L S      + S+ D+MTWLS+A+T 
Sbjct: 128 TITYTQM--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187

Query: 200 HDTCTAGFEDVADLG--VKDEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRL 259
           HDTCT GF+++   G  VKD+V   + DLSEM+SN LAIF+G       +PV N   R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKL 247

Query: 260 MESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIE 319
           +  GT         + E  P W+  +DR LL  P SAIQADI V+KDGSG FKT+AEAI+
Sbjct: 248 L--GTE--------ETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307

Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
            AP  S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367

Query: 380 ATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
           ATFA +G G I+RDMTFEN AGP+KHQAVALR+  DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427

Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
           FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487

Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
           +AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEY 543

BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 5.0e-174
Identity = 313/556 (56.29%), Postives = 405/556 (72.84%), Query Frame = 0

Query: 13  SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSRIA 72
           S + +TT A           P +++ R + ++++ S  +++ LI A+ + A +  R ++ 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 73  SSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSA-NVHDLVHISLNLTLQHLT 132
            S P   R KPSQAIS+AC  T +P LC+ SL+ FPG+ +A +  DL+H+++N+TL H +
Sbjct: 74  QSVPGLAR-KPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 133 KALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSTEASNHDIMTWLS 192
            ALYS++ +    +   P A SAY+ C+ELL+D++DA S    S+ S+ A   D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 193 AALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
           AALT HDTCT GF+ V D GVKD + A L +LSE++SN LAIFS    GD    +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 253 KRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTV 312
           +   + E              E FP W+  K+R +L  P+S IQADI+V+KDG+G  KT+
Sbjct: 254 RLLGVEER------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313

Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
           +EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373

Query: 373 TTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
           TTF TA+FA +G G I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433

Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
           SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493

Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
           HA +++A  DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553

Query: 553 YYGEYMNSGPGPPWGR 560
           YYGEY+NSGPG   G+
Sbjct: 554 YYGEYLNSGPGSGLGQ 554

BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 409.1 bits (1050), Expect = 8.4e-113
Identity = 231/551 (41.92%), Postives = 339/551 (61.52%), Query Frame = 0

Query: 37  SLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISR-ACSHTLY 96
           + ++  +++++S    LL  A+V      + +  + +      +  S A+ R +CS T Y
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAG----ISAGASKANEKRTLSPSSHAVLRSSCSSTRY 72

Query: 97  PTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 156
           P LC+S++++  G +  +  D++  S+NLT+  +    ++  ++   +    P   +A  
Sbjct: 73  PELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALH 132

Query: 157 DCMELLNDAIDAF-----SLSLFSTEAS--NH--DIMTWLSAALTYHDTCTAGF-EDVAD 216
           DC+E +++ +D        L L+ T+ +   H  D+ T +S+A+T  +TC  GF  D AD
Sbjct: 133 DCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDAD 192

Query: 217 LGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRKRRRLMESGTPSWAA 276
             V+  +      +  M SN+LA+       D+        + +  R+   E+   + A 
Sbjct: 193 KQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAV 252

Query: 277 ENGG----DHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSS 336
           +  G    D EG+P W+S  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +S
Sbjct: 253 DIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENS 312

Query: 337 GRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGS 396
            +R +I+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A  
Sbjct: 313 NKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 372

Query: 397 GTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDI 456
           G   + RD+TF+NTAGPSKHQAVALR+ +D +  Y+C+++ YQDTLYVHSNRQFF +C I
Sbjct: 373 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 432

Query: 457 YGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDL 516
            GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I AT DL
Sbjct: 433 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 492

Query: 517 EASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 566
           ++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G  
Sbjct: 493 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGA- 552

BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.9e-112
Identity = 239/554 (43.14%), Postives = 336/554 (60.65%), Query Frame = 0

Query: 25  RLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQ 84
           +LS +      S++ ++ + L ++  + LL+ A+V+     V SR  S       N+P  
Sbjct: 10  KLSESSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHH 69

Query: 85  AISR-ACSHTLYPTLCLSSLLSFPGAQS--ANVHDLVHISLNLTLQHLTKALYSTSQIPI 144
           AI + +CS T YP LC S++ + P A     +  D++ +SLN+T   +    +   ++ +
Sbjct: 70  AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-L 129

Query: 145 LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTE-------ASNH--DIMTWLSAALTY 204
            + +       A  DC+E +++ +D    ++   E        S H  D+ T +SAA+T 
Sbjct: 130 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 189

Query: 205 HDTCTAGF-EDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMES 264
             TC  GF  D A+  V+D +      + +M SN+LA+       D+ +      R +  
Sbjct: 190 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKL-- 249

Query: 265 GTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAP 324
                  E     +G+P W+S  DRRLL +  S++  + VVA DGSG FKTVA A+ AAP
Sbjct: 250 ------TEETSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAP 309

Query: 325 SSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATF 384
               +R II IKAG Y  EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT 
Sbjct: 310 QGGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATA 369

Query: 385 AGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRE 444
           A  G G + RD+TF+NTAGPSKHQAVALR+ AD +  Y+C+++ YQDTLYVHSNRQFF  
Sbjct: 370 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 429

Query: 445 CDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVAT 504
           C I GT+DFIFGNA  V Q+C+I+ARKP +GQKN +TAQ R DPNQNTGI I   +I AT
Sbjct: 430 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGAT 489

Query: 505 GDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNS 564
            DL+  +GSFPT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ NS
Sbjct: 490 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNS 544

Query: 565 GPGPPWGRGSSGRV 566
           G     G G+SGRV
Sbjct: 550 GA----GAGTSGRV 544

BLAST of PI0003165 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 3.2e-112
Identity = 237/554 (42.78%), Postives = 337/554 (60.83%), Query Frame = 0

Query: 25  RLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQ 84
           +LS +      S++ ++ + L ++  + LL+ A+V+     V SR  S       N+P  
Sbjct: 10  KLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHH 69

Query: 85  AISR-ACSHTLYPTLCLSSLLSFPGAQS--ANVHDLVHISLNLTLQHLTKALYSTSQIPI 144
           AI + +CS T YP LC S++ + P A     +  D++ +SLN+T   +    +   ++ +
Sbjct: 70  AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-L 129

Query: 145 LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTE-------ASNH--DIMTWLSAALTY 204
            + +       A  DC+E +++ +D    ++   E        S H  D+ T +SAA+T 
Sbjct: 130 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 189

Query: 205 HDTCTAGF-EDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMES 264
             TC  GF  D A+  V+D +      + +M SN+LA+       D+ +      R +  
Sbjct: 190 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKL-- 249

Query: 265 GTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAP 324
                  E     +G+P W+S  DRRLL +  S++  ++VVA DGSG FKTVA ++ AAP
Sbjct: 250 ------IEETSTVDGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAP 309

Query: 325 SSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATF 384
               +R II IKAG Y  EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT 
Sbjct: 310 QGGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 369

Query: 385 AGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRE 444
           A  G G + RD+TF+NTAGPSKHQAVALR+ AD +  Y+C+++ YQDTLYVHSNRQFF  
Sbjct: 370 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 429

Query: 445 CDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVAT 504
           C I GT+DFIFGNA  V Q+C+I+ARKP +GQKN +TAQ R DPNQNTGI I   +I AT
Sbjct: 430 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGAT 489

Query: 505 GDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNS 564
            DL+  +GSFPT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N+
Sbjct: 490 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNA 544

Query: 565 GPGPPWGRGSSGRV 566
           G     G G+SGRV
Sbjct: 550 GA----GAGTSGRV 544

BLAST of PI0003165 vs. ExPASy TrEMBL
Match: A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 2.1e-308
Identity = 537/559 (96.06%), Postives = 547/559 (97.85%), Query Frame = 0

Query: 1   MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNN+YNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 120
           SAAILVRS + SSQPHFP NKPSQAISRACSHTLYP+LCLSSLLSFPGAQSA+VHDLVHI
Sbjct: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 180
           SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS +ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCTAGF+DVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 300
           ENRKRRRLMES T SWAAENGGDHEGFP W+SGKDRRLLAAP+S IQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300

Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
           KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360

Query: 361 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
           DKVTTFRTATFAGSGT IILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420

Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480

Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
           ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540

Query: 541 DTLYYGEYMNSGPGPPWGR 560
           DTLYYGEYMNSGPG   G+
Sbjct: 541 DTLYYGEYMNSGPGAAVGQ 559

BLAST of PI0003165 vs. ExPASy TrEMBL
Match: A0A1S3AUZ6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 2.0e-306
Identity = 539/560 (96.25%), Postives = 547/560 (97.68%), Query Frame = 0

Query: 1   MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNNNY +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 120
           SAAILVRSRIASSQPHFP NKPSQAISRACSHTLYP+LCLSSLLSFPGAQSA+VHDLVHI
Sbjct: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 180
           SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFST ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCT GF+DV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESGTP-SWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGK 300
           ENRKRRRLMESGTP SWAAENGGDHEGFPEW+SGKDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
           YDKVTTFRTATFAGSGTGIILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGPPWGR 560
           LDTLYYGEYMNSGPG   G+
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQ 560

BLAST of PI0003165 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 7.5e-266
Identity = 475/559 (84.97%), Postives = 509/559 (91.06%), Query Frame = 0

Query: 4   NNNYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NNNYN LH+SHS + TPA+SFR+S   +DP PP S    R RIL+ISF S+LLI AS VS
Sbjct: 2   NNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61

Query: 64  AAILVRSRI-ASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 123
           A +LV SRI AS++ HFP NKP+QAISRACS TLYP+LCLSSLLS PG+ SA+VHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 183
           SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP WVSGKDRRLLAAPIS+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYMNSGPGPPWGR 560
           DTLYYGEYMNSGPG   G+
Sbjct: 542 DTLYYGEYMNSGPGAAVGQ 557

BLAST of PI0003165 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.3e-262
Identity = 471/562 (83.81%), Postives = 505/562 (89.86%), Query Frame = 0

Query: 4   NNNYNKLHTSHSANTTPAASFRLSGN-----DPPPPSSSLRRRSRILIISFMSVLLIAAS 63
           NNNYNKLH+S S + TP  SFR+S +      PPPPSSS R   RIL+ISF S+LLI AS
Sbjct: 2   NNNYNKLHSSRSGD-TPVTSFRVSNDHSNPPPPPPPSSSHR---RILLISFFSILLITAS 61

Query: 64  VVSAAILVRSRI-ASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDL 123
            VSA +LV SRI  S++ HFP NKP+QAISRACS TLYP+LC+SSLLS PG+ SA+VHDL
Sbjct: 62  TVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDL 121

Query: 124 VHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEA 183
           VHISLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A
Sbjct: 122 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 181

Query: 184 SNHDIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD 243
           +NHD+MTWLSAALTYHDTCT GF DVAD  VKD++ AKLSDLSEMISNSLAIFSGFGGGD
Sbjct: 182 TNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGGD 241

Query: 244 LPVENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGS 303
           LPVENR+RRRLMES     A EN GD + FP WVSGKDRRLLAAPIS+IQADIVVAKDGS
Sbjct: 242 LPVENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGS 301

Query: 304 GKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 363
           GKFKTVAEAI AAPSSS RRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 302 GKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 361

Query: 364 SVYDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 423
           SVYDKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADH VV+  N+IGYQ
Sbjct: 362 SVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQ 421

Query: 424 DTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQ 483
           DTLYVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQ
Sbjct: 422 DTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQ 481

Query: 484 NTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 543
           NTGISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 482 NTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGS 541

Query: 544 FALDTLYYGEYMNSGPGPPWGR 560
           FALDTLYYGEYMNSGP    G+
Sbjct: 542 FALDTLYYGEYMNSGPSAAVGQ 557

BLAST of PI0003165 vs. ExPASy TrEMBL
Match: A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.7e-254
Identity = 463/557 (83.12%), Postives = 500/557 (89.77%), Query Frame = 0

Query: 5   NNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAI 64
           NNYNKLH+S S +T   A+F++S    PP SS+ RRR+RIL +S +S+LLI AS VSAA+
Sbjct: 2   NNYNKLHSSASGDT--PATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAV 61

Query: 65  LVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHISLNL 124
           L+ S+      HFP NKP+QAIS ACSHTLYP+LC SSLLSFPG+ SA+VH LVHISLNL
Sbjct: 62  LLHSQ----SSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNL 121

Query: 125 TLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNHDIMT 184
           TLQHLTKALY++SQIP+ QIS +PLAHSAY+DC+ELLNDA+DAFSLSLFST ASNHDIMT
Sbjct: 122 TLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMT 181

Query: 185 WLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRK 244
           WLSAALTYHDTC+ GF DV D  VKD++ A+LSDLSEMISNSLAIFSG+G G LPV+N  
Sbjct: 182 WLSAALTYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGYGEG-LPVQN-- 241

Query: 245 RRRLMESGTPSWAAENG-GDHEG-FPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 304
           RRRLMES    WAAENG    EG FP WVSGKDRRLLAAPISAIQADIVVAKDGSGKFKT
Sbjct: 242 RRRLMESDR-EWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 301

Query: 305 VAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDK 364
           VAEAIEAAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+DK
Sbjct: 302 VAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDK 361

Query: 365 VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYV 424
           VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALR+SADHAVVY CNIIGYQDTLYV
Sbjct: 362 VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYV 421

Query: 425 HSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGIS 484
           HSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM  QKNTITAQ+RKDPNQNTGIS
Sbjct: 422 HSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGIS 481

Query: 485 IHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT 544
           IHACKI+AT DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT
Sbjct: 482 IHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT 541

Query: 545 LYYGEYMNSGPGPPWGR 560
           LYYGEYMNSGPG   G+
Sbjct: 542 LYYGEYMNSGPGAATGQ 547

BLAST of PI0003165 vs. NCBI nr
Match: XP_011654619.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.1 hypothetical protein Csa_000423 [Cucumis sativus])

HSP 1 Score: 1067.4 bits (2759), Expect = 4.3e-308
Identity = 537/559 (96.06%), Postives = 547/559 (97.85%), Query Frame = 0

Query: 1   MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNN+YNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 120
           SAAILVRS + SSQPHFP NKPSQAISRACSHTLYP+LCLSSLLSFPGAQSA+VHDLVHI
Sbjct: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 180
           SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS +ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCTAGF+DVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 300
           ENRKRRRLMES T SWAAENGGDHEGFP W+SGKDRRLLAAP+S IQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300

Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
           KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360

Query: 361 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
           DKVTTFRTATFAGSGT IILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420

Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480

Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
           ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540

Query: 541 DTLYYGEYMNSGPGPPWGR 560
           DTLYYGEYMNSGPG   G+
Sbjct: 541 DTLYYGEYMNSGPGAAVGQ 559

BLAST of PI0003165 vs. NCBI nr
Match: XP_008437596.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])

HSP 1 Score: 1060.8 bits (2742), Expect = 4.0e-306
Identity = 539/560 (96.25%), Postives = 547/560 (97.68%), Query Frame = 0

Query: 1   MNNNNNYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNNNY +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 120
           SAAILVRSRIASSQPHFP NKPSQAISRACSHTLYP+LCLSSLLSFPGAQSA+VHDLVHI
Sbjct: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 180
           SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFST ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCT GF+DV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESGTP-SWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGK 300
           ENRKRRRLMESGTP SWAAENGGDHEGFPEW+SGKDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
           YDKVTTFRTATFAGSGTGIILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGPPWGR 560
           LDTLYYGEYMNSGPG   G+
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQ 560

BLAST of PI0003165 vs. NCBI nr
Match: XP_038874841.1 (probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida])

HSP 1 Score: 988.4 bits (2554), Expect = 2.5e-284
Identity = 503/559 (89.98%), Postives = 528/559 (94.45%), Query Frame = 0

Query: 4   NNNYNKLHTSHSANTTPAASFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63
           NNNYNKLH+S SA +TPA+SF LSGND    PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 3   NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62

Query: 64  SAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 123
           SAA+LVRSRIAS++PHFP NKPSQAISRACSHTLYP+LCLSSLLS P A SA+VHDLVHI
Sbjct: 63  SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122

Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 183
           SLNLTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFS+SLFSTEASNH
Sbjct: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182

Query: 184 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVADL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242

Query: 244 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 303
           +NR RRRLMESGT SWAAENGGD  GFP+W+S KDRRLLAAPISAIQADIVVAKDGSGKF
Sbjct: 243 QNR-RRRLMESGTRSWAAENGGD-GGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 302

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI+AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+
Sbjct: 303 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 362

Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGTGIILRDM FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDTL
Sbjct: 363 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 422

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNTG
Sbjct: 423 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 482

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFAL
Sbjct: 483 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 542

Query: 544 DTLYYGEYMNSGPGPPWGR 560
           DTLYYGEY NSGPG   G+
Sbjct: 543 DTLYYGEYSNSGPGAAVGQ 559

BLAST of PI0003165 vs. NCBI nr
Match: XP_022922302.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])

HSP 1 Score: 926.0 bits (2392), Expect = 1.6e-265
Identity = 475/559 (84.97%), Postives = 509/559 (91.06%), Query Frame = 0

Query: 4   NNNYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NNNYN LH+SHS + TPA+SFR+S   +DP PP S    R RIL+ISF S+LLI AS VS
Sbjct: 2   NNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61

Query: 64  AAILVRSRI-ASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 123
           A +LV SRI AS++ HFP NKP+QAISRACS TLYP+LCLSSLLS PG+ SA+VHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 183
           SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP WVSGKDRRLLAAPIS+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYMNSGPGPPWGR 560
           DTLYYGEYMNSGPG   G+
Sbjct: 542 DTLYYGEYMNSGPGAAVGQ 557

BLAST of PI0003165 vs. NCBI nr
Match: KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 924.5 bits (2388), Expect = 4.5e-265
Identity = 474/559 (84.79%), Postives = 509/559 (91.06%), Query Frame = 0

Query: 4   NNNYNKLHTSHSANTTPAASFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NNNYNKLH+S S + TPA+SFR+S   +DP PP S    R RIL+ISF+S+ LI AS VS
Sbjct: 2   NNNYNKLHSSRSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFLSIFLITASAVS 61

Query: 64  AAILVRSRI-ASSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHI 123
           A +LV SRI AS++ HFP NKP+QAISRACS TLYP+LCLSSLLS PG+ SA+VHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTEASNH 183
           SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP+WVSGKDRRLLAAPIS+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEHWLAEENNGDGD-FPQWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYMNSGPGPPWGR 560
           DTLYYGEYMNSGPG   G+
Sbjct: 542 DTLYYGEYMNSGPGAAVGQ 557

BLAST of PI0003165 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 623.6 bits (1607), Expect = 1.5e-178
Identity = 327/551 (59.35%), Postives = 402/551 (72.96%), Query Frame = 0

Query: 20  PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPR 79
           P+     +  DP      L+++++  +I F   +L+   VV   I    R   S    P+
Sbjct: 8   PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPK 67

Query: 80  --NKPSQAISRACSHTLYPTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTS 139
              KP+QAISR CS +LYP LC+ +LL FPG+ +A+ ++L+HIS N TLQ  +KALY++S
Sbjct: 68  LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127

Query: 140 QIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFST-----EASNHDIMTWLSAALTY 199
            I   Q+   P   SAY+ C+ELL+D++DA + +L S      + S+ D+MTWLS+A+T 
Sbjct: 128 TITYTQM--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187

Query: 200 HDTCTAGFEDVADLG--VKDEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRL 259
           HDTCT GF+++   G  VKD+V   + DLSEM+SN LAIF+G       +PV N   R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKL 247

Query: 260 MESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIE 319
           +  GT         + E  P W+  +DR LL  P SAIQADI V+KDGSG FKT+AEAI+
Sbjct: 248 L--GTE--------ETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307

Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
            AP  S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367

Query: 380 ATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
           ATFA +G G I+RDMTFEN AGP+KHQAVALR+  DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427

Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
           FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487

Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
           +AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEY 543

BLAST of PI0003165 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 612.5 bits (1578), Expect = 3.5e-175
Identity = 313/556 (56.29%), Postives = 405/556 (72.84%), Query Frame = 0

Query: 13  SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSRIA 72
           S + +TT A           P +++ R + ++++ S  +++ LI A+ + A +  R ++ 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 73  SSQPHFPRNKPSQAISRACSHTLYPTLCLSSLLSFPGAQSA-NVHDLVHISLNLTLQHLT 132
            S P   R KPSQAIS+AC  T +P LC+ SL+ FPG+ +A +  DL+H+++N+TL H +
Sbjct: 74  QSVPGLAR-KPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 133 KALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSTEASNHDIMTWLS 192
            ALYS++ +    +   P A SAY+ C+ELL+D++DA S    S+ S+ A   D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 193 AALTYHDTCTAGFEDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
           AALT HDTCT GF+ V D GVKD + A L +LSE++SN LAIFS    GD    +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 253 KRRRLMESGTPSWAAENGGDHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTV 312
           +   + E              E FP W+  K+R +L  P+S IQADI+V+KDG+G  KT+
Sbjct: 254 RLLGVEER------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313

Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
           +EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373

Query: 373 TTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
           TTF TA+FA +G G I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433

Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
           SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493

Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
           HA +++A  DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553

Query: 553 YYGEYMNSGPGPPWGR 560
           YYGEY+NSGPG   G+
Sbjct: 554 YYGEYLNSGPGSGLGQ 554

BLAST of PI0003165 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 409.1 bits (1050), Expect = 5.9e-114
Identity = 231/551 (41.92%), Postives = 339/551 (61.52%), Query Frame = 0

Query: 37  SLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISR-ACSHTLY 96
           + ++  +++++S    LL  A+V      + +  + +      +  S A+ R +CS T Y
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAG----ISAGASKANEKRTLSPSSHAVLRSSCSSTRY 72

Query: 97  PTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 156
           P LC+S++++  G +  +  D++  S+NLT+  +    ++  ++   +    P   +A  
Sbjct: 73  PELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALH 132

Query: 157 DCMELLNDAIDAF-----SLSLFSTEAS--NH--DIMTWLSAALTYHDTCTAGF-EDVAD 216
           DC+E +++ +D        L L+ T+ +   H  D+ T +S+A+T  +TC  GF  D AD
Sbjct: 133 DCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDAD 192

Query: 217 LGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRKRRRLMESGTPSWAA 276
             V+  +      +  M SN+LA+       D+        + +  R+   E+   + A 
Sbjct: 193 KQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAV 252

Query: 277 ENGG----DHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSS 336
           +  G    D EG+P W+S  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +S
Sbjct: 253 DIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENS 312

Query: 337 GRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGS 396
            +R +I+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A  
Sbjct: 313 NKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 372

Query: 397 GTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDI 456
           G   + RD+TF+NTAGPSKHQAVALR+ +D +  Y+C+++ YQDTLYVHSNRQFF +C I
Sbjct: 373 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 432

Query: 457 YGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDL 516
            GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I AT DL
Sbjct: 433 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 492

Query: 517 EASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 566
           ++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G  
Sbjct: 493 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGA- 552

BLAST of PI0003165 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 406.0 bits (1042), Expect = 5.0e-113
Identity = 231/543 (42.54%), Postives = 321/543 (59.12%), Query Frame = 0

Query: 36  SSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISRACSHTLY 95
           S  +   ++++ S    LL+ AS+V  A    ++  + +     +     +   CS TLY
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLY 71

Query: 96  PTLCLSSLLSFPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 155
           P LC S++ +  G +  +  +++  SLNLT + +    ++  ++   +    P   +A  
Sbjct: 72  PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 131

Query: 156 DCMELLNDAIDAFSLSL---------FSTEASNHDIMTWLSAALTYHDTCTAGFE-DVAD 215
           DC+E +++ +D   +++          S      D+ T +S+A+T   TC  GF  D AD
Sbjct: 132 DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDAD 191

Query: 216 LGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESGTPSWAAENGG-- 275
             V+  +      +  M SN+LA+       D+   E R +     +       E  G  
Sbjct: 192 RKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDL 251

Query: 276 DHEGFPEWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYI 335
           D +G+P+W+S  DRRLL    S I+AD  VA DGSG F TVA A+ AAP  S +R +I+I
Sbjct: 252 DSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHI 311

Query: 336 KAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIILRD 395
           KAG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + RD
Sbjct: 312 KAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARD 371

Query: 396 MTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIF 455
           +TF+NTAGPSKHQAVALR+ +D +  Y C++  YQDTLYVHSNRQFF +C I GT+DFIF
Sbjct: 372 ITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIF 431

Query: 456 GNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFP 515
           GNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  T DL A KG+FP
Sbjct: 432 GNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFP 491

Query: 516 TFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGPPWGRGSS 566
           T+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G     G G++
Sbjct: 492 TYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRG----GGAGTA 547

BLAST of PI0003165 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 372.9 bits (956), Expect = 4.7e-103
Identity = 221/529 (41.78%), Postives = 302/529 (57.09%), Query Frame = 0

Query: 37  SLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPRNKPSQAISRACSHTLYP 96
           SL+ ++  L++SF+++L   A   +  I V +           N  S   +    H  + 
Sbjct: 14  SLKHKNLCLVLSFVAILGSVAFFTAQLISVNTN---------NNDDSLLTTSQICHGAHD 73

Query: 97  TLCLSSLLS---FPGAQSANVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSA 156
                +LLS          N  DL+H+ L  ++  L   +   S+  I   S      + 
Sbjct: 74  QDSCQALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIR--SNGVRDKAG 133

Query: 157 YEDCMELLNDAIDAFSLSLFSTEASNH------DIMTWLSAALTYHDTCTAGFEDVADLG 216
           + DC E+++ + D    S+      N+      ++ TWLS+ LT + TC     DV+ + 
Sbjct: 134 FADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVS-VN 193

Query: 217 VKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESGTPSWAAENGGDHEGF 276
            K  V+ +L DL      +LAIF       LP  +  +  +                  F
Sbjct: 194 SKQIVKPQLEDLVSRARVALAIFVSV----LPARDDLKMIISNR---------------F 253

Query: 277 PEWVSGKDRRLLAAPISAIQ--ADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYIKAG 336
           P W++  DR+LL +    ++  A++VVAKDG+GKFKTV EA+ AAP +S  R +IY+K G
Sbjct: 254 PSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKG 313

Query: 337 KYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIILRDMTF 396
            Y +E + +G+KK NLM VGDGK  T+I+G  +V D  TTFR+AT A +G G + +D+ F
Sbjct: 314 VY-KETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWF 373

Query: 397 ENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIFGNA 456
           +NTAGP+KHQAVALR+SAD  V+  C I  YQDTLY H+ RQF+R+  I GT+DFIFGN+
Sbjct: 374 QNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNS 433

Query: 457 VVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFPTFL 516
            VVFQ+C+I AR P AGQKN +TAQ R+D NQNT ISI  CKI A+ DL   KGS  TFL
Sbjct: 434 AVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFL 493

Query: 517 GRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 555
           GRPWKLYSR V M S + +HI P GW  W G FAL TLYYGEY N+GPG
Sbjct: 494 GRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPG 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FK052.2e-17759.35Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B05.0e-17456.29Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... [more]
O490068.4e-11341.92Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
O048861.9e-11243.14Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
P839483.2e-11242.78Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KJG12.1e-30896.06Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1[more]
A0A1S3AUZ62.0e-30696.25Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1[more]
A0A6J1E2V17.5e-26684.97Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1[more]
A0A6J1IA522.3e-26283.81Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1[more]
A0A6J1H2K11.7e-25483.12Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011654619.14.3e-30896.06probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.... [more]
XP_008437596.14.0e-30696.25PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
XP_038874841.12.5e-28489.98probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida][more]
XP_022922302.11.6e-26584.97probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata][more]
KAG6579321.14.5e-26584.79putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
AT5G53370.11.5e-17859.35pectin methylesterase PCR fragment F [more]
AT3G49220.13.5e-17556.29Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G14310.15.9e-11441.92pectin methylesterase 3 [more]
AT1G53830.15.0e-11342.54pectin methylesterase 2 [more]
AT1G11580.14.7e-10341.78methylesterase PCR A [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 81..229
e-value: 1.7E-41
score: 153.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 54..231
e-value: 1.2E-32
score: 110.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 85..229
e-value: 6.5E-31
score: 107.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 83..233
e-value: 3.2E-36
score: 126.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 84..231
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 283..568
e-value: 6.8E-107
score: 359.5
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 289..555
e-value: 5.4E-131
score: 435.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availablePANTHERPTHR31707:SF184PECTINESTERASEcoord: 37..559
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 37..559
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 89..235
e-value: 1.30004E-43
score: 150.666
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 434..443
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 284..559

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003165.1PI0003165.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity