PI0003121 (gene) Melon (PI 482460) v1

Overview
NamePI0003121
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionpolygalacturonase-like
Locationchr09: 12654857 .. 12658415 (-)
RNA-Seq ExpressionPI0003121
SyntenyPI0003121
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCTTGTTGAAAACTTGCACCTTCTTTCCCTTCTGCTTCTATTATTTCTTAACCACGGTTTGGCTGCTGGATCGACATTCAACATCGTTAGTCTTGGAGCCAAACCCGATGGCGAGACCGACGCCTCGCCTGCATTTCAAGAGGCATGGGCCAATGCCTGTGGCTCGTCAGAACCGGCCACCATTTACGTGCCGGACGGTACATTCTACGTTCAAAGTGGGAACTTTATTGGACCTTGTAAGAACAATGCTATTACTATTCTCATCAATGGCACTCTTATGGCTTCTTCAAATATTCAAGTTTTAGCTCAATCTCAAGCTTGGATAGCATTTAGAAACATAAATGGGCTTTCCATTTATGGAGGTGTTATCGATGGTCAAGGAATTGGGTTATGGAATTGTAAGCATTCAGGAAAGAGTTGCCCTGATGGAGCCACGGTATGCAAAAAAGTTTGAAACAAAAATGCAAGGTTGAATTTTGATTTGTACTGATTATTACTGTTGTTCATTGTTTTGAATGTGCGTATGTAGAATCTTGAAATCTTCAATGCACAAAATGTAAGTGTGAATGGATTGTCTTCGGTTAATAGCCAAATGTTCAATATCGTGGTGCATGGTTGTCAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCCGGCGATAGTCCAAACACCGATGGCATTCATGTCCAACAATCATTGAACGTGAACATATTGAACTCAAGCATTGGCACCGGCGACGACTGCATTTCAATTGGTCCAGGAACTACCAACTTGTGGATGGAAGATATTAAATGTGGGCCTGGACATGGAATCAGGTAAAGTAGGGATAGATGACAATAATTTTTATGTTTGGTAGTGTTTTTGAAAGTGCTACAATCATTTTTATACCTTTAAAATCGCTCTATAAGCATACCATCAATTACTTAAAATCAATATTGATTAAATAAAAATTGAGTTTGATGTGTATAGAAATAACAAGACAATAAGACATTCTTTGCTAACTAAAATATTTTTGGTTTTCTATTTTAAAAAAAAAAAATTGTGCTTTTTCCATACAATATTTTAGGATTGTTTTTAATTATGTAAAATATCATTGATAGATGTTGATAGACTATTGTCATCTATCACTAATAAATGTTCATAGATACTAATGAAACAACGTCTATCACTCTCTATTACTTTAGAAGAAAAAAAGTGGTCGCAGTCATACTAAAAGGATTAATTGGTCTTGAATAGTTATAGCTATGGATCGTTTTAATTTCGAAACAACAATGATTATTATTATTATGTACATCTAACTGAACAAACAAAACTATGTGCACCAAACTTCCACATGTAGTAAAATAAGATAAAATAATCATTTTTAATAAAAAAAATGCAAATCTATCACATGTCAAATTCTTACTATTTGTAGTGATCTGGGCGACACAAAAATGAGTACAACTCATATGATTAAAACTCATATAATAATAACAATTTCGAAATCATCCTCAAACATATTCTAAACACACAACTCAAAGTGATATTAACCTTTAGAAAAGTAGTCGGAAAGAAGTTAAAAGTAGTTACACAACATATTTTTGTTATAAACTTGAGAGAATTTTTCATACCACCCTCAATTTATTACTTGTCCCCATCTACTAGCATTTAAGAAAAATCTAGGGTTCATAAATTAAATGAATATACATCAATGGGTAACATGTGAAATTATGAACGAGAGTAAACTACATGTTGTGTAATTGTAGCATTGGGAGCTTGGGGAAGGATGCAGAAGAGGCTGGAGTGGAAAACGTGACGGTAACAACTGCAACTTTCAGAGGGACTCAAAATGGAGTGAGGATTAAGTCTTGGGAAAGGGCAAGCCATGGGTTCGCTACAAAAATTCTTTTTCAACGCATTCTTTTTTATAATGTCAGCAATCCTATCTTCATTGATCAAAATTATTGTCCAAATCACCAACCCTGTCCGGGTCAGGTACGTTATCCAAACACGAGTAATCATCTTCTGCTTTTGAAAAAAATTTACATTTGTTAAAATATCTTCTCTTTAATATACATATCATTTTGTTGTTTAAATTTGTCCTTTTAAACGTTATGATATGAATAATTAATTTACTTTAATAGAAAATTAATTGTATTCGACATAACCTTTTATGTATCTCTATAATTGATTGATAATTGTCTATTTTAGTCATCATCAGCATTAATTTAATTTCATTTTAGAGATTTCATATAATTGGCTCGTCCTCACTTATATTATAATATTCTCCTTGCATAGGAAAACATGCCACTTTGAATATTGCATTTGATTTATATATCATTGCTGCATTTTATGGTTAGTTTAAATGTTGCATTTTCTTGCTTCAAATTGTTGCTAAATTTGACTTTTAGTGGATTTTGACATTCTTTTTTCTTGAGTTTAAATTAGTTTTGTTTCCTGAATTCTGTTGCATTAATAGGCTAATTTATATTTTGTTTTATTATATGAACATTTTAAATTTCACATCTCTTTTATTCACAAAATAAAAAATATAACGTTGAAAATGACTCCCTAATTATTTATTTGGAACTTCTTAGATTTTTAAAAATTTCAAATGGATTTCATTGTTGTCATTATGGTCTAGAGAGTGATTTGAATCTTGTTTATACTTAAAATCTTTTCCGACTAAGATGCTCGGATTTTGGAGGGTTTCATAGAATTTAAAAATAAATTACTTAGGGCATGTTTGGTAACCCATTTGTATTTTATTTTTCTATTTTGAAAATTAATTTTTAAAAATCAAGTGTGTTTGGTAGCTAATTCACTTTTTAATTTAATTTTTTAAATAAGAAACTTGTTTTATTAAAAATTATGAAGAAAAATTGAAAACGAAAAAATTCTTTCAAAAACAAAAACAAAAATCAATTTACCAAACATATATGAATTTTGTTTTTAATAAACAAAATTAAATTAAGGAACAAAAATTGAAATGGTTACCGGACGAGGTTTTTAATTAACCTTATCATATTTTAATTTTTAATTAACACATCATTATTAAAATGTTATATTAGTAAATTCTTTAACTTTCTTTTATTTTTCACTTATCATCAAAATACAAAATTTGTAATAAATCCATTTCAAATATTTATGGTATGATCCAAGCAAAGTAATTAGTTTTAAATTATTCCTCAAATTTCAAATAGCATCATTTAAAATCGTTTCTTAAACTTTCTTGAAATCTAAAACAAACGACTAATAAAATAAAGTAAACATGGTTAAAAAAAATTGAAGGCTAATGTATCCTTGTTTATTCAATTTCATAACATTATGATTTTGTTTGTGTGACTAGGCTTCCGGAGTTAAAGTTAGCAATGTGACGTATCAAGACATTAACGGAACATCAGCTACTGAAGTAGCAATCAATTTTGACTGTAGTCCAACAAATCCATGCACTGGAATAAGGTTGGAAGACATAAATCTCACTTACAACAATCAAATTCCACAAGCTTCATGTAAAAATGCTCAAGGAACTGCCTATGGTCCTCTTCAACCTTCTAGTTGCTTGGCTTAG

mRNA sequence

ATGCCTCTTGTTGAAAACTTGCACCTTCTTTCCCTTCTGCTTCTATTATTTCTTAACCACGGTTTGGCTGCTGGATCGACATTCAACATCGTTAGTCTTGGAGCCAAACCCGATGGCGAGACCGACGCCTCGCCTGCATTTCAAGAGGCATGGGCCAATGCCTGTGGCTCGTCAGAACCGGCCACCATTTACGTGCCGGACGGTACATTCTACGTTCAAAGTGGGAACTTTATTGGACCTTGTAAGAACAATGCTATTACTATTCTCATCAATGGCACTCTTATGGCTTCTTCAAATATTCAAGTTTTAGCTCAATCTCAAGCTTGGATAGCATTTAGAAACATAAATGGGCTTTCCATTTATGGAGGTGTTATCGATGGTCAAGGAATTGGGTTATGGAATTGTAAGCATTCAGGAAAGAGTTGCCCTGATGGAGCCACGAATCTTGAAATCTTCAATGCACAAAATGTAAGTGTGAATGGATTGTCTTCGGTTAATAGCCAAATGTTCAATATCGTGGTGCATGGTTGTCAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCCGGCGATAGTCCAAACACCGATGGCATTCATGTCCAACAATCATTGAACGTGAACATATTGAACTCAAGCATTGGCACCGGCGACGACTGCATTTCAATTGGTCCAGGAACTACCAACTTGTGGATGGAAGATATTAAATGTGGGCCTGGACATGGAATCAGCATTGGGAGCTTGGGGAAGGATGCAGAAGAGGCTGGAGTGGAAAACGTGACGGTAACAACTGCAACTTTCAGAGGGACTCAAAATGGAGTGAGGATTAAGTCTTGGGAAAGGGCAAGCCATGGGTTCGCTACAAAAATTCTTTTTCAACGCATTCTTTTTTATAATGTCAGCAATCCTATCTTCATTGATCAAAATTATTGTCCAAATCACCAACCCTGTCCGGGTCAGGCTTCCGGAGTTAAAGTTAGCAATGTGACGTATCAAGACATTAACGGAACATCAGCTACTGAAGTAGCAATCAATTTTGACTGTAGTCCAACAAATCCATGCACTGGAATAAGGTTGGAAGACATAAATCTCACTTACAACAATCAAATTCCACAAGCTTCATGTAAAAATGCTCAAGGAACTGCCTATGGTCCTCTTCAACCTTCTAGTTGCTTGGCTTAG

Coding sequence (CDS)

ATGCCTCTTGTTGAAAACTTGCACCTTCTTTCCCTTCTGCTTCTATTATTTCTTAACCACGGTTTGGCTGCTGGATCGACATTCAACATCGTTAGTCTTGGAGCCAAACCCGATGGCGAGACCGACGCCTCGCCTGCATTTCAAGAGGCATGGGCCAATGCCTGTGGCTCGTCAGAACCGGCCACCATTTACGTGCCGGACGGTACATTCTACGTTCAAAGTGGGAACTTTATTGGACCTTGTAAGAACAATGCTATTACTATTCTCATCAATGGCACTCTTATGGCTTCTTCAAATATTCAAGTTTTAGCTCAATCTCAAGCTTGGATAGCATTTAGAAACATAAATGGGCTTTCCATTTATGGAGGTGTTATCGATGGTCAAGGAATTGGGTTATGGAATTGTAAGCATTCAGGAAAGAGTTGCCCTGATGGAGCCACGAATCTTGAAATCTTCAATGCACAAAATGTAAGTGTGAATGGATTGTCTTCGGTTAATAGCCAAATGTTCAATATCGTGGTGCATGGTTGTCAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCCGGCGATAGTCCAAACACCGATGGCATTCATGTCCAACAATCATTGAACGTGAACATATTGAACTCAAGCATTGGCACCGGCGACGACTGCATTTCAATTGGTCCAGGAACTACCAACTTGTGGATGGAAGATATTAAATGTGGGCCTGGACATGGAATCAGCATTGGGAGCTTGGGGAAGGATGCAGAAGAGGCTGGAGTGGAAAACGTGACGGTAACAACTGCAACTTTCAGAGGGACTCAAAATGGAGTGAGGATTAAGTCTTGGGAAAGGGCAAGCCATGGGTTCGCTACAAAAATTCTTTTTCAACGCATTCTTTTTTATAATGTCAGCAATCCTATCTTCATTGATCAAAATTATTGTCCAAATCACCAACCCTGTCCGGGTCAGGCTTCCGGAGTTAAAGTTAGCAATGTGACGTATCAAGACATTAACGGAACATCAGCTACTGAAGTAGCAATCAATTTTGACTGTAGTCCAACAAATCCATGCACTGGAATAAGGTTGGAAGACATAAATCTCACTTACAACAATCAAATTCCACAAGCTTCATGTAAAAATGCTCAAGGAACTGCCTATGGTCCTCTTCAACCTTCTAGTTGCTTGGCTTAG

Protein sequence

MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA
Homology
BLAST of PI0003121 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 1.8e-135
Identity = 232/388 (59.79%), Postives = 295/388 (76.03%), Query Frame = 0

Query: 6   NLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYV 65
           +L  LSL+ +  +N  +A+  T+N+ SLGAK DG+TD++ AF  AWA AC S  P  IYV
Sbjct: 6   SLFSLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYV 65

Query: 66  PDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYGGVI 125
           P GTF+++   F GPCKNNAIT  I GTL+A S+ +V+  +  WI F ++NG++I GG++
Sbjct: 66  PAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGIL 125

Query: 126 DGQGIGLWNCKH-SGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQG 185
           DGQG  LW CK   G+SCP GAT L   ++ N+ V+GL+S+NSQMF+IV++  QNVQ+QG
Sbjct: 126 DGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQG 185

Query: 186 VNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGIS 245
           V VS +G+SPNTDGIHVQ S  V ILNS I TGDDC+SIGPGT+NLW+E + CGPGHGIS
Sbjct: 186 VRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGIS 245

Query: 246 IGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPI 305
           IGSLGK+ EEAGV+NVTV T TF GTQNG+RIKSW R S GFA  ILFQ     NV NPI
Sbjct: 246 IGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPI 305

Query: 306 FIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINL 365
            IDQ+YCP+++ CPGQ SGV++S+VTY+DI+GTSATEVA+ FDCSP +PC  I+LED+ L
Sbjct: 306 VIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKL 365

Query: 366 TYNNQIPQASCKNAQGTAYGPLQPSSCL 393
           TY NQ  ++SC +A GT  G +QP+SCL
Sbjct: 366 TYKNQAAESSCSHADGTTEGVVQPTSCL 393

BLAST of PI0003121 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.4e-106
Identity = 201/388 (51.80%), Postives = 267/388 (68.81%), Query Frame = 0

Query: 7   LHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYVP 66
           L +L   L+  L H +   ST N++S GAKPDG  D++ AF  AW  AC S+ P TI VP
Sbjct: 14  LIILDFTLISSLAHPIP--STLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVP 73

Query: 67  DGTFYVQSGNFIG-PCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYGGVI 126
            G F V +  F G  CK   I+I I G+++A  + +++A S+ WI F ++  +SIYGG++
Sbjct: 74  KGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGIL 133

Query: 127 DGQGIGLWNCKHS-GKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQG 186
           D QG  LW CK++ G +CP GA +L    + N+ ++GL+S+NSQ F+IV+    NV I G
Sbjct: 134 DAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDG 193

Query: 187 VNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGIS 246
           V VS+  +SPNTDGIHV+ S +V+I NS IGTGDDCISIGPG+TN++++ I+CGPGHGIS
Sbjct: 194 VKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGIS 253

Query: 247 IGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPI 306
           IGSLG+  EE GV+NVTV+   F GT NGVRIK+W + S+ FA  I+FQ I    V NPI
Sbjct: 254 IGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPI 313

Query: 307 FIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINL 366
            IDQ+YC  H+PCP Q SGVKVSNV Y+DI+GTS TEVA+  DCS   PCTGI ++D+NL
Sbjct: 314 IIDQHYC-LHKPCPKQESGVKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNL 373

Query: 367 TYNNQIPQASCKNAQGTAYGPLQPSSCL 393
              ++  QASC NA G+A   +  + CL
Sbjct: 374 VSVHRPAQASCDNANGSANDVVPFTPCL 398

BLAST of PI0003121 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.9e-76
Identity = 167/410 (40.73%), Postives = 245/410 (59.76%), Query Frame = 0

Query: 1   MPLVEN-LHLLSLLLLLFLNHGLAA------------GSTFNIVSLGAKPDGETDASPAF 60
           M  ++N +  L LL L  + HG  A            G +F+I  LGA  +G+TD++ A 
Sbjct: 1   MACIDNAMRALFLLALFCVVHGEKAKSKDNDAKASGPGGSFDITKLGASGNGKTDSTKAV 60

Query: 61  QEAWANACGSSEPATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQ 120
           QEAWA+ACG +   TI +P G F V   NF GPCK + +TI +NG L+A++++       
Sbjct: 61  QEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGD-VTIQVNGNLLATTDLSQYKDHG 120

Query: 121 AWIAFRNINGLSIYG-GVIDGQGIGLWNCKHSGK--SCPDGATNLEIFNAQNVSVNGLSS 180
            WI    ++ L I G G +DGQG  +W+     K   C     +L +    N  V+G++ 
Sbjct: 121 NWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSGITL 180

Query: 181 VNSQMFNIVVHGCQNVQIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIG 240
           +NS+ F++ ++ C+++ I+ VNV++ GDSPNTDGIH+  S  V I N+ IG GDDCISIG
Sbjct: 181 LNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIG 240

Query: 241 PGTTNLWMEDIKCGPGHGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASH 300
           PGT+ + +  + CGPGHGISIGSLG+  +E  V ++ V   T + T NGVRIK++E A+ 
Sbjct: 241 PGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAAS 300

Query: 301 GF-ATKILFQRILFYNVSNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVA 360
              A+KI ++ I   +   PI ID  YCPN       AS V V +VT+++I GTS+T  A
Sbjct: 301 VLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEA 360

Query: 361 INFDCSPTNPCTGIRLEDINLTYN--NQIPQASCKNAQGTAYGPLQPSSC 392
           +N  C+   PCTG+ ++D+N+ Y+  N    A CKNA+G+A G L+  +C
Sbjct: 361 VNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKGSAKGCLKELAC 409

BLAST of PI0003121 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 287.3 bits (734), Expect = 2.5e-76
Identity = 165/379 (43.54%), Postives = 221/379 (58.31%), Query Frame = 0

Query: 17  FLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYVPDG-TFYVQSG 76
           F+   L   +T ++   GAK DG+TD + AF  AW  AC S+    + VP G T+ ++S 
Sbjct: 57  FVRASLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSI 116

Query: 77  NFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYG---GVIDGQGIGL 136
              GPC N+ +T+ I GTL AS           WI F  +N LS+ G   GV+DG G   
Sbjct: 117 QLTGPC-NSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETW 176

Query: 137 W--NCK-HSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQGVNVSS 196
           W  +CK +  K C    T L  +N++++ V  L   N+Q   I +  C NVQ+  V V++
Sbjct: 177 WQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTA 236

Query: 197 AGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGISIGSLG 256
             DSPNTDGIH+  + N+ +  S IGTGDDCISI  G+ N+ + DI CGPGHGISIGSLG
Sbjct: 237 PADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLG 296

Query: 257 KDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPIFIDQN 316
            D  +A V  VTV  A   GT NGVRIK+++  S G A+ I+FQ I   NV NPI IDQ+
Sbjct: 297 DDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGS-GTASNIIFQNIQMDNVKNPIIIDQD 356

Query: 317 YCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINLTYNNQ 376
           YC +   C  + S V+V NV Y+DI+GTSA+E AI F+CS   PC GI L+ +N+     
Sbjct: 357 YC-DKSKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIKGG-- 416

Query: 377 IPQASCKNAQGTAYGPLQP 389
             +A+C NA     G + P
Sbjct: 417 --KATCTNANVVDKGAVLP 428

BLAST of PI0003121 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.8e-75
Identity = 164/384 (42.71%), Postives = 223/384 (58.07%), Query Frame = 0

Query: 12  LLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYVPDG-TF 71
           +L L+  +      ST ++ + GAK DG+TD + AF++AW  AC ++   T  VP G T+
Sbjct: 52  VLSLISSDETTLEASTVSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTY 111

Query: 72  YVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYG---GVIDG 131
            ++S  F GPCK +     I GTL AS+          W+   ++N LSI G   G+I+G
Sbjct: 112 LLKSTRFRGPCK-SLRNFQILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIING 171

Query: 132 QGIGLW--NCK-HSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQG 191
            G   W  +CK    K C    T L ++N +N++V  L   N+Q   I +  C  V++  
Sbjct: 172 NGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSN 231

Query: 192 VNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGIS 251
           V +++ GDSPNTDGIH+  + N+ + NS IGTGDDCISI  GT NL + D+ CGPGHGIS
Sbjct: 232 VEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGIS 291

Query: 252 IGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPI 311
           IGSLG D  +A V  + V  A F  + NGVRIK+++  S G A  I FQ I   NV NPI
Sbjct: 292 IGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGS-GTAKNIKFQNIRMENVKNPI 351

Query: 312 FIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINL 371
            IDQ+YC +   C  Q S V+V NV Y++I+GTSAT+VAI  +CS   PC GI LE++ +
Sbjct: 352 IIDQDYC-DKDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKI 411

Query: 372 TYNNQIPQASCKNAQGTAYGPLQP 389
                   ASCKNA     G + P
Sbjct: 412 KGGT----ASCKNANVKNQGTVSP 428

BLAST of PI0003121 vs. ExPASy TrEMBL
Match: A0A5D3DS41 (Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001940 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.0e-201
Identity = 351/393 (89.31%), Postives = 370/393 (94.15%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           MPLV NLHLL  LL LFLNHGLAAGSTFNIVSLGAK DG TDASPAFQEAWANACGSSEP
Sbjct: 1   MPLVGNLHLL-FLLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
           ATIYVP+G FYV SGNF GPCKNNAIT+LINGTLMASSNIQVLAQSQAWIAFR+INGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITVLINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
           YGGVIDGQG+GLWNCKHSG+SCPDGAT+LEIF++QNV+VNGLSSVNSQMFNIVV+GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
           QIQGVNVSSA DSPNTDGIHVQQSLNVNI NS+IGTGDDCISIGPGTTNLWME+I CGPG
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLGK AEEAGV+NVTVTTATFRGTQNGVRIKSW R S GFATKILFQ ILFYNV
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
           SNPIFIDQNYCP+ Q CPG+ASGVK+S+VTYQDINGTSATEVAINFDCSP+NPCTGI LE
Sbjct: 301 SNPIFIDQNYCPHQQSCPGRASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLE 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DINL YNNQIPQASC+NAQGTA GPLQP SCLA
Sbjct: 361 DINLNYNNQIPQASCENAQGTASGPLQPRSCLA 392

BLAST of PI0003121 vs. ExPASy TrEMBL
Match: A0A1S3CCA2 (polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103499335 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.0e-201
Identity = 352/393 (89.57%), Postives = 370/393 (94.15%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           MPLV NLHLL  LL LFLNHGLAAGSTFNIVSLGAK DG TDASPAFQEAWANACGSSEP
Sbjct: 1   MPLVGNLHLL-FLLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
           ATIYVP+G FYV SGNF GPCKNNAITILINGTLMASSNIQVLAQSQAWIAFR+INGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
           YGGVIDGQG+GLWNCKHSG+SCPDGAT+LEIF++QNV+VNGLSSVNSQMFNIVV+GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
           QIQGVNVSSA DSPNTDGIHVQQSLNVNI NS+IGTGDDCISIGPGTTNLWME+I CGPG
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLGK AEEAGV+NVTVTTATFRGTQNGVRIKSW R S GFATKILFQ ILFYNV
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
           SNPIFIDQNYCP+ Q CPGQASGVK+S+VTYQDINGTSATEVAINFDCSP+NPCTGI LE
Sbjct: 301 SNPIFIDQNYCPHQQSCPGQASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLE 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DINL YN+QIPQASC+NAQGTA GPLQP SCLA
Sbjct: 361 DINLNYNDQIPQASCENAQGTASGPLQPRSCLA 392

BLAST of PI0003121 vs. ExPASy TrEMBL
Match: A0A0A0KN91 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G409610 PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.5e-196
Identity = 344/393 (87.53%), Postives = 366/393 (93.13%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           MPLV NL LLS LL LFLNHGLAAGSTFNIVSLGA  DG+TDAS AFQEAWANACGSSEP
Sbjct: 1   MPLVGNLPLLS-LLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
           ATIYVP+G FY+QSGNF GPCKNNAITI+INGTL+ASSNIQVLAQSQAWIAFR INGLSI
Sbjct: 61  ATIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
           YGGVIDGQGIGLWNCKHSGKSCPDGATNLEI +AQNV+VNGLSS+NSQMFNIVV+GC+NV
Sbjct: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
           QIQGVNVSSAGDSPNTDGIHVQQSLNVNI ++SIGTGDDCISIGPGTTNLWME+IKCGPG
Sbjct: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLG+  EEAGVENVTVTTATF GTQNGVRIKSW R S+ FA KILFQ I FYNV
Sbjct: 241 HGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKILFQHIRFYNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
           +NPI IDQNYCPN+Q CPGQASGVKVS+VTYQD+NGTSA+EVAINFDCSPTNPCTGI LE
Sbjct: 301 NNPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLE 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DI LTYNNQIP+ASCKNA+GTA GPLQP SCLA
Sbjct: 361 DIQLTYNNQIPKASCKNARGTASGPLQPRSCLA 392

BLAST of PI0003121 vs. ExPASy TrEMBL
Match: A0A6J1F386 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439563 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.8e-163
Identity = 284/393 (72.26%), Postives = 330/393 (83.97%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           M L+  LH+   LLLLF NH  A G TFNIV+LGAK DG+ D SP  Q AW  AC SS+P
Sbjct: 1   MALLGTLHVFLSLLLLFFNHAWAVGLTFNIVNLGAKSDGKIDVSPVLQTAWTKACDSSKP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
            TIYVP G FYV+S +F GPCKNNAIT+LI+GTL+AS NIQVLA++++W+ F +INGLSI
Sbjct: 61  TTIYVPKGIFYVRSVSFNGPCKNNAITLLIDGTLVASPNIQVLARTKSWLEFSHINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
            GGVIDGQG  LW+CK SGKSCP+GA +L+I + +NV++  LSS+NSQMFNIVVHGCQNV
Sbjct: 121 VGGVIDGQGTALWDCKRSGKSCPNGARSLQINDTKNVNIWSLSSINSQMFNIVVHGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
            + GV+VSSAGDSPNTDGIHVQQS NV ILNS+IGTGDDCISIGPGT+NLWME+I CGPG
Sbjct: 181 HMTGVSVSSAGDSPNTDGIHVQQSSNVTILNSNIGTGDDCISIGPGTSNLWMENITCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLGK AEE GV NVTV ++TF GTQNGVRIKSW R S+GFATK+ FQRI+  NV
Sbjct: 241 HGISIGSLGKGAEEDGVHNVTVISSTFTGTQNGVRIKSWGRPSNGFATKVYFQRIIMDNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
            NPI ID+NYCP+H+ CPGQASGVKVSNVTY+DI GTSATE+AINFDCSP N CTG+ L+
Sbjct: 301 KNPIIIDENYCPHHKNCPGQASGVKVSNVTYEDITGTSATEIAINFDCSPINHCTGLNLK 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DI LTY NQI QASCKNA+GTA+G ++PSSCLA
Sbjct: 361 DIKLTYENQIAQASCKNAEGTAFGVVEPSSCLA 393

BLAST of PI0003121 vs. ExPASy TrEMBL
Match: A0A6J1IDZ8 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111474610 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.9e-160
Identity = 283/393 (72.01%), Postives = 329/393 (83.72%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           M L+ +LH+   LLLLF NH LA G TFNIVSLGAK DG+ D S   Q AWA AC SS+P
Sbjct: 1   MALLRSLHVFLSLLLLFFNHALAVGLTFNIVSLGAKSDGKIDVSSVLQIAWAKACDSSKP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
            TIYVP G FYV+S +F GPCKNNAIT+LI+GTL+ASSNIQVLA+++ W+ F +INGLSI
Sbjct: 61  TTIYVPKGIFYVRSVSFNGPCKNNAITLLIDGTLVASSNIQVLARTKFWLEFNHINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
            GGVIDGQG  LW+CK SGKSCP+GA +L+I + +NV++ GLSS+NSQMFNIVV GCQNV
Sbjct: 121 VGGVIDGQGTALWDCKRSGKSCPNGARSLQINDTKNVNIWGLSSINSQMFNIVVDGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
            + GV+VSSAGDSPNTDGIHVQQS NV ILNS+IGTGDDCISIGPGT+NLW+E+I CGPG
Sbjct: 181 HMTGVSVSSAGDSPNTDGIHVQQSSNVTILNSNIGTGDDCISIGPGTSNLWIENITCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLGK AEE GV NVTV ++TF GTQNGVRIKSW R S+GFATK+ FQRI+  NV
Sbjct: 241 HGISIGSLGKRAEEDGVHNVTVISSTFTGTQNGVRIKSWGRPSNGFATKVHFQRIIMDNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
            NPI ID+NYCP+H+ CPGQASGVKVS+VTY+DI GTSATE+AINFDCSP N CT + L+
Sbjct: 301 KNPIIIDENYCPHHKNCPGQASGVKVSDVTYEDIKGTSATEIAINFDCSPINHCTRLSLK 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DI LTY NQI QASCKNA+G A G ++PSSCLA
Sbjct: 361 DIKLTYENQIAQASCKNAEGIASGVVEPSSCLA 393

BLAST of PI0003121 vs. NCBI nr
Match: XP_008460550.1 (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 711.4 bits (1835), Expect = 4.2e-201
Identity = 352/393 (89.57%), Postives = 370/393 (94.15%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           MPLV NLHLL  LL LFLNHGLAAGSTFNIVSLGAK DG TDASPAFQEAWANACGSSEP
Sbjct: 1   MPLVGNLHLL-FLLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
           ATIYVP+G FYV SGNF GPCKNNAITILINGTLMASSNIQVLAQSQAWIAFR+INGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
           YGGVIDGQG+GLWNCKHSG+SCPDGAT+LEIF++QNV+VNGLSSVNSQMFNIVV+GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
           QIQGVNVSSA DSPNTDGIHVQQSLNVNI NS+IGTGDDCISIGPGTTNLWME+I CGPG
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLGK AEEAGV+NVTVTTATFRGTQNGVRIKSW R S GFATKILFQ ILFYNV
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
           SNPIFIDQNYCP+ Q CPGQASGVK+S+VTYQDINGTSATEVAINFDCSP+NPCTGI LE
Sbjct: 301 SNPIFIDQNYCPHQQSCPGQASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLE 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DINL YN+QIPQASC+NAQGTA GPLQP SCLA
Sbjct: 361 DINLNYNDQIPQASCENAQGTASGPLQPRSCLA 392

BLAST of PI0003121 vs. NCBI nr
Match: KAA0062404.1 (polygalacturonase-like [Cucumis melo var. makuwa] >TYK26577.1 polygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 711.4 bits (1835), Expect = 4.2e-201
Identity = 351/393 (89.31%), Postives = 370/393 (94.15%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           MPLV NLHLL  LL LFLNHGLAAGSTFNIVSLGAK DG TDASPAFQEAWANACGSSEP
Sbjct: 1   MPLVGNLHLL-FLLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
           ATIYVP+G FYV SGNF GPCKNNAIT+LINGTLMASSNIQVLAQSQAWIAFR+INGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITVLINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
           YGGVIDGQG+GLWNCKHSG+SCPDGAT+LEIF++QNV+VNGLSSVNSQMFNIVV+GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
           QIQGVNVSSA DSPNTDGIHVQQSLNVNI NS+IGTGDDCISIGPGTTNLWME+I CGPG
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLGK AEEAGV+NVTVTTATFRGTQNGVRIKSW R S GFATKILFQ ILFYNV
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
           SNPIFIDQNYCP+ Q CPG+ASGVK+S+VTYQDINGTSATEVAINFDCSP+NPCTGI LE
Sbjct: 301 SNPIFIDQNYCPHQQSCPGRASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLE 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DINL YNNQIPQASC+NAQGTA GPLQP SCLA
Sbjct: 361 DINLNYNNQIPQASCENAQGTASGPLQPRSCLA 392

BLAST of PI0003121 vs. NCBI nr
Match: XP_004140371.1 (polygalacturonase [Cucumis sativus])

HSP 1 Score: 694.5 bits (1791), Expect = 5.3e-196
Identity = 344/393 (87.53%), Postives = 366/393 (93.13%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           MPLV NL LLS LL LFLNHGLAAGSTFNIVSLGA  DG+TDAS AFQEAWANACGSSEP
Sbjct: 1   MPLVGNLPLLS-LLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
           ATIYVP+G FY+QSGNF GPCKNNAITI+INGTL+ASSNIQVLAQSQAWIAFR INGLSI
Sbjct: 61  ATIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
           YGGVIDGQGIGLWNCKHSGKSCPDGATNLEI +AQNV+VNGLSS+NSQMFNIVV+GC+NV
Sbjct: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
           QIQGVNVSSAGDSPNTDGIHVQQSLNVNI ++SIGTGDDCISIGPGTTNLWME+IKCGPG
Sbjct: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLG+  EEAGVENVTVTTATF GTQNGVRIKSW R S+ FA KILFQ I FYNV
Sbjct: 241 HGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKILFQHIRFYNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
           +NPI IDQNYCPN+Q CPGQASGVKVS+VTYQD+NGTSA+EVAINFDCSPTNPCTGI LE
Sbjct: 301 NNPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLE 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DI LTYNNQIP+ASCKNA+GTA GPLQP SCLA
Sbjct: 361 DIQLTYNNQIPKASCKNARGTASGPLQPRSCLA 392

BLAST of PI0003121 vs. NCBI nr
Match: XP_038887074.1 (polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 658.7 bits (1698), Expect = 3.2e-185
Identity = 317/393 (80.66%), Postives = 357/393 (90.84%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           MP++  L LL  LLLLF  H LA G TF+IVSLGAKP+G+TD+SPAFQ AW NAC SS P
Sbjct: 1   MPVLGTLQLLLSLLLLFFTHDLATGLTFDIVSLGAKPNGKTDSSPAFQTAWTNACSSSVP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
           ATIYVP+G FYVQSGNF GPCKNNAITILI+GTLMASS+IQVLAQ+++WI+F++INGLSI
Sbjct: 61  ATIYVPNGVFYVQSGNFNGPCKNNAITILIDGTLMASSDIQVLAQTESWISFKDINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
            GGV+DGQGIGLWNCKHSG+SCPDGATNL I NAQNV V+GLSSVNSQMFNIVVHGCQNV
Sbjct: 121 SGGVLDGQGIGLWNCKHSGQSCPDGATNLLISNAQNVKVSGLSSVNSQMFNIVVHGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
            +QG+NVS AGDSPNTDGIHV+QSLNV ILNSSIGTGDDCISIGPGTTNLWME+I CGPG
Sbjct: 181 LVQGMNVSDAGDSPNTDGIHVEQSLNVTILNSSIGTGDDCISIGPGTTNLWMENITCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HG+SIGSLGK+AEEAGVENVTV+TATFRGT+NGVRIKSW R S GFAT +LFQ I+FY+V
Sbjct: 241 HGVSIGSLGKEAEEAGVENVTVSTATFRGTENGVRIKSWGRPSDGFATNVLFQHIVFYDV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
            NP+ IDQNYCPNH+ CPGQASGVK+S+VTYQD+NGTSATEVAINF+CSP+NPCTG+ L+
Sbjct: 301 KNPLIIDQNYCPNHESCPGQASGVKISDVTYQDMNGTSATEVAINFNCSPSNPCTGLALD 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DI LTYNN IPQASC+NAQGTA GP+QPSSCLA
Sbjct: 361 DIKLTYNNGIPQASCENAQGTASGPVQPSSCLA 393

BLAST of PI0003121 vs. NCBI nr
Match: XP_022932948.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 584.7 bits (1506), Expect = 5.9e-163
Identity = 284/393 (72.26%), Postives = 330/393 (83.97%), Query Frame = 0

Query: 1   MPLVENLHLLSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEP 60
           M L+  LH+   LLLLF NH  A G TFNIV+LGAK DG+ D SP  Q AW  AC SS+P
Sbjct: 1   MALLGTLHVFLSLLLLFFNHAWAVGLTFNIVNLGAKSDGKIDVSPVLQTAWTKACDSSKP 60

Query: 61  ATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSI 120
            TIYVP G FYV+S +F GPCKNNAIT+LI+GTL+AS NIQVLA++++W+ F +INGLSI
Sbjct: 61  TTIYVPKGIFYVRSVSFNGPCKNNAITLLIDGTLVASPNIQVLARTKSWLEFSHINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNV 180
            GGVIDGQG  LW+CK SGKSCP+GA +L+I + +NV++  LSS+NSQMFNIVVHGCQNV
Sbjct: 121 VGGVIDGQGTALWDCKRSGKSCPNGARSLQINDTKNVNIWSLSSINSQMFNIVVHGCQNV 180

Query: 181 QIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPG 240
            + GV+VSSAGDSPNTDGIHVQQS NV ILNS+IGTGDDCISIGPGT+NLWME+I CGPG
Sbjct: 181 HMTGVSVSSAGDSPNTDGIHVQQSSNVTILNSNIGTGDDCISIGPGTSNLWMENITCGPG 240

Query: 241 HGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNV 300
           HGISIGSLGK AEE GV NVTV ++TF GTQNGVRIKSW R S+GFATK+ FQRI+  NV
Sbjct: 241 HGISIGSLGKGAEEDGVHNVTVISSTFTGTQNGVRIKSWGRPSNGFATKVYFQRIIMDNV 300

Query: 301 SNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLE 360
            NPI ID+NYCP+H+ CPGQASGVKVSNVTY+DI GTSATE+AINFDCSP N CTG+ L+
Sbjct: 301 KNPIIIDENYCPHHKNCPGQASGVKVSNVTYEDITGTSATEIAINFDCSPINHCTGLNLK 360

Query: 361 DINLTYNNQIPQASCKNAQGTAYGPLQPSSCLA 394
           DI LTY NQI QASCKNA+GTA+G ++PSSCLA
Sbjct: 361 DIKLTYENQIAQASCKNAEGTAFGVVEPSSCLA 393

BLAST of PI0003121 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 448.0 bits (1151), Expect = 8.0e-126
Identity = 210/384 (54.69%), Postives = 284/384 (73.96%), Query Frame = 0

Query: 10  LSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYVPDGT 69
           L ++LL  L+   ++  T+NI+S GAKPDG+TD++ AF   WA AC S +P TI VP G 
Sbjct: 5   LLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKGR 64

Query: 70  FYVQSGNFIG-PCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYGGVIDGQ 129
           F ++S  F G  CK  ++T  I GTL+A S+ +V+ +   WI F++++G+S+YGGV+D Q
Sbjct: 65  FLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDAQ 124

Query: 130 GIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQGVNVS 189
           G  LW+CK SGK+CP GAT++   +++NV ++GL+S+NSQMF++ ++GC NV++ GV VS
Sbjct: 125 GASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKVS 184

Query: 190 SAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGISIGSL 249
           + G+SPNTDGIHVQ S  V+ILNS I TGDDC+SIGPGT  LW+E++ CGPGHGISIGSL
Sbjct: 185 ADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 244

Query: 250 GKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPIFIDQ 309
           GK++ E GV+N+TV TATF GT+NGVRIKSW R S+GFA  I FQ  +  NV NPI IDQ
Sbjct: 245 GKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVIDQ 304

Query: 310 NYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINLTYNN 369
           NYCP ++ CP Q SG+K+S+V + DI+GTSATEV +  DCS   PCTGIR++D+ LTY N
Sbjct: 305 NYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLTYRN 364

Query: 370 QIPQASCKNAQGTAYGPLQPSSCL 393
           +     C +A G+  G  +P+SCL
Sbjct: 365 KPATTDCSHAGGSEAGFQRPNSCL 388

BLAST of PI0003121 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 439.1 bits (1128), Expect = 3.7e-123
Identity = 210/382 (54.97%), Postives = 277/382 (72.51%), Query Frame = 0

Query: 12  LLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYVPDGTFY 71
           L+L +F      +  ++N++S GAKPDG+TDA+ AF   W  AC SS P TI VP G F 
Sbjct: 5   LVLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRFL 64

Query: 72  VQSGNFIG-PCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYGGVIDGQGI 131
           ++S  F G  CK   +T  I+GTL+A ++ +V+     WI F++++G+++YGGV+D +G 
Sbjct: 65  LRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARGA 124

Query: 132 GLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQGVNVSSA 191
            LW+CK SGK+CP GAT +   ++ NV V+GL+S+NSQMF++V++GC NV++QGV V +A
Sbjct: 125 SLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVLAA 184

Query: 192 GDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGISIGSLGK 251
           G+SPNTDGIHVQ S +V+I N+ I TGDDC+SIGPGT  LW+E++ CGPGHGISIGSLGK
Sbjct: 185 GNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSLGK 244

Query: 252 DAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPIFIDQNY 311
           D+ E+GV+NVTV T TF GT NGVRIKSW R S GFA  I FQ  +  NV NPI IDQNY
Sbjct: 245 DSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQNY 304

Query: 312 CPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINLTYNNQI 371
           CP+H  CP Q SG+K+S+V + DI+GTSATEV +  DCS   PCTGIRLED+ LTY N+ 
Sbjct: 305 CPDHD-CPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQNKP 364

Query: 372 PQASCKNAQGTAYGPLQPSSCL 393
             ++C +A G   G  QP +CL
Sbjct: 365 AASACTHAGGIEAGFFQP-NCL 384

BLAST of PI0003121 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.0e-109
Identity = 193/393 (49.11%), Postives = 263/393 (66.92%), Query Frame = 0

Query: 4   VENLHLLSLLLLLF----LNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSE 63
           ++N  L+++LL+ F    L     A S +N+VS GAKPDG TD++ AF  AW  AC S+ 
Sbjct: 1   MDNNKLVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAA 60

Query: 64  PATIYVPDGTFYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLS 123
             T+ VP G+F ++   F GPC++  IT  I GT++A S+ + L  S  WI F  +N +S
Sbjct: 61  AVTVTVPRGSFLLKPVEFRGPCRSR-ITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRIS 120

Query: 124 IYGGVIDGQGIGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQN 183
           I GG +D +G   W C+ SGKSCP GA ++    A +V V+GL+S+NSQ  ++V++ C N
Sbjct: 121 IIGGTLDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNN 180

Query: 184 VQIQGVNVSSAGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGP 243
           V ++ V + +   SPNTDG+HVQ S  V + + +  TGDDCISIGPGT NL+M  + CGP
Sbjct: 181 VIVRKVKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGP 240

Query: 244 GHGISIGSLGKDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYN 303
           GHGISIGSLG+DA EAGVEN+T+  + F G+ NGVRIK+W R S GF   +LFQ ++  N
Sbjct: 241 GHGISIGSLGRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKN 300

Query: 304 VSNPIFIDQNYCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRL 363
           V NPI +DQNYCP++Q CP Q SGVK+S V Y++I GTS T+ A+ FDCS +NPC  IRL
Sbjct: 301 VQNPIIVDQNYCPSNQGCPKQGSGVKISQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRL 360

Query: 364 EDINLTYNNQIPQASCKNAQGTAYGPLQPSSCL 393
            DI LT+N +   ++CKN +G   G + P  CL
Sbjct: 361 HDIKLTFNGRSATSTCKNIKGVKAGVVMPQGCL 392

BLAST of PI0003121 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 393.7 bits (1010), Expect = 1.8e-109
Identity = 188/384 (48.96%), Postives = 263/384 (68.49%), Query Frame = 0

Query: 10  LSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYVPDGT 69
           L   LL F++  ++A + FN+VS GAKPDG TD++ AF +AW  AC S+  AT+ VP GT
Sbjct: 10  LLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKGT 69

Query: 70  FYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYGGVIDGQG 129
           F ++   F GPCK+  IT  + GT++A  + +    S  WI F  +N  S+ GG  D + 
Sbjct: 70  FLLKVITFGGPCKSK-ITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDARA 129

Query: 130 IGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQGVNVSS 189
            G W+C+ SG++CP G  ++   +A++V ++G+ S+NSQ+ ++ ++GC NV ++ V + +
Sbjct: 130 NGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVA 189

Query: 190 AGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGISIGSLG 249
            G+SPNTDG HVQ S  V    S++ TGDDC++IGPGT NL +  + CGPGHG+SIGSL 
Sbjct: 190 PGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGSLA 249

Query: 250 KDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPIFIDQN 309
           K+ +E GVENVTV+++ F G+QNGVRIKSW R S+GF   + FQ ++  NV NPI IDQN
Sbjct: 250 KELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIIDQN 309

Query: 310 YCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINLTYNNQ 369
           YCP H+ CP + SGVK+S VTY++I GTSAT+ A+   CS ++PCTGI L+DI LTYN  
Sbjct: 310 YCPTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTYNKG 369

Query: 370 IPQAS-CKNAQGTAYGPLQPSSCL 393
            P  S C NA G + G +QP+SCL
Sbjct: 370 TPATSFCFNAVGKSLGVIQPTSCL 392

BLAST of PI0003121 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 389.4 bits (999), Expect = 3.4e-108
Identity = 187/384 (48.70%), Postives = 259/384 (67.45%), Query Frame = 0

Query: 10  LSLLLLLFLNHGLAAGSTFNIVSLGAKPDGETDASPAFQEAWANACGSSEPATIYVPDGT 69
           L   LL F++   +A   FN+VS GAKPDG TD++ AF +AW  ACGS+  AT+ VP GT
Sbjct: 10  LIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVVPTGT 69

Query: 70  FYVQSGNFIGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRNINGLSIYGGVIDGQG 129
           F ++   F GPCK+  IT  + GT++A  + +    S +WI F  +N  S+ GG  D +G
Sbjct: 70  FLLKVITFGGPCKSK-ITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGTFDARG 129

Query: 130 IGLWNCKHSGKSCPDGATNLEIFNAQNVSVNGLSSVNSQMFNIVVHGCQNVQIQGVNVSS 189
            G W+C+ SG++CP G  ++   +A++V ++G+ S+NSQ+ ++ ++GC NV ++ + + +
Sbjct: 130 SGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRNIRLVA 189

Query: 190 AGDSPNTDGIHVQQSLNVNILNSSIGTGDDCISIGPGTTNLWMEDIKCGPGHGISIGSLG 249
            GDSPNTDG  VQ S  V +  S++ TGDDC++IG GT N  +  + CGPGHG+SIGSL 
Sbjct: 190 PGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVSIGSLA 249

Query: 250 KDAEEAGVENVTVTTATFRGTQNGVRIKSWERASHGFATKILFQRILFYNVSNPIFIDQN 309
           K   E GVENVTV+++ F G+QNGVRIKSW R S GF   + FQ ++  NV NPI IDQN
Sbjct: 250 KQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPIIIDQN 309

Query: 310 YCPNHQPCPGQASGVKVSNVTYQDINGTSATEVAINFDCSPTNPCTGIRLEDINLTYNNQ 369
           YCP++Q CP + SGVK++ VTY++I GTSAT+ A+   CS +NPCTGI L+DI LTYN  
Sbjct: 310 YCPSNQGCPTEHSGVKITQVTYKNIQGTSATQEAMKLVCSKSNPCTGITLQDIKLTYNKG 369

Query: 370 IPQAS-CKNAQGTAYGPLQPSSCL 393
            P  S C NA G   G +QP+SCL
Sbjct: 370 TPATSLCFNAVGKNLGVIQPTSCL 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P489791.8e-13559.79Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228182.4e-10651.80Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
P353391.9e-7640.73Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1[more]
Q8RY292.5e-7643.54Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
O231474.8e-7542.71Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DS412.0e-20189.31Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
A0A1S3CCA22.0e-20189.57polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103499335 PE=3 SV=1[more]
A0A0A0KN912.5e-19687.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G409610 PE=3 SV=1[more]
A0A6J1F3862.8e-16372.26polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439563 PE=3 SV=1[more]
A0A6J1IDZ82.9e-16072.01polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111474610 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008460550.14.2e-20189.57PREDICTED: polygalacturonase-like [Cucumis melo][more]
KAA0062404.14.2e-20189.31polygalacturonase-like [Cucumis melo var. makuwa] >TYK26577.1 polygalacturonase-... [more]
XP_004140371.15.3e-19687.53polygalacturonase [Cucumis sativus][more]
XP_038887074.13.2e-18580.66polygalacturonase-like [Benincasa hispida][more]
XP_022932948.15.9e-16372.26polygalacturonase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G59850.18.0e-12654.69Pectin lyase-like superfamily protein [more]
AT2G43870.13.7e-12354.97Pectin lyase-like superfamily protein [more]
AT2G43890.11.0e-10949.11Pectin lyase-like superfamily protein [more]
AT1G05660.11.8e-10948.96Pectin lyase-like superfamily protein [more]
AT1G05650.13.4e-10848.70Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 204..225
e-value: 2200.0
score: 4.8
coord: 257..278
e-value: 53.0
score: 12.7
coord: 321..361
e-value: 1800.0
score: 5.4
coord: 287..308
e-value: 6000.0
score: 1.7
coord: 177..203
e-value: 9.4
score: 15.2
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 55..379
e-value: 8.3E-86
score: 288.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 234..247
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 20..390
e-value: 6.6E-114
score: 383.1
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 8..391
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 8..391
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 12..392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003121.1PI0003121.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity